IVF0021483 (gene) Melon (IVF77) v1

BLAST of IVF0021483 vs. ExPASy Swiss-Prot
Match: Q9C5V6 (DNA topoisomerase 6 subunit B OS=Arabidopsis thaliana OX=3702 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 547/662 (82.63%), Postives = 605/662 (91.39%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT A+Y VLK+M + H +K+KR+  ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDAT-DNFTDFQSPLFVFR 671
            ETL++KLAEHVEQVD+EMA+EYATQSG S EP+E +Y+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LI 673
           L+
Sbjct: 668 LM 669

BLAST of IVF0021483 vs. ExPASy Swiss-Prot
Match: B8BDQ4 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. indica OX=39946 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 1.6e-304
Identity = 524/677 (77.40%), Postives = 597/677 (88.18%), Query Frame = 0

Query: 3   AGGGSESPDEPKKGKSKTPRK-------PKESILKQKSPAEFFAENKNIAGFDNPGKCLY 62
           A   S +      GK K   K        + S+LKQKSPAEFFAENKNIAGFDNPGK LY
Sbjct: 20  AASSSAAAKGKAAGKGKAASKASALATAKESSLLKQKSPAEFFAENKNIAGFDNPGKSLY 79

Query: 63  TTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKA 122
           TT+RELVEN+LDSAESISELP +EI IEEI K+KFN+MIGLVDR+R+DEELYDD+E+AKA
Sbjct: 80  TTMRELVENALDSAESISELPDIEIIIEEITKSKFNTMIGLVDRQRIDEELYDDFESAKA 139

Query: 123 REKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIP 182
           REKRLAKEAR QE QAKNAALGKK +E  A++G KGRGEA+F+RVTCKDNG+GMPHDDIP
Sbjct: 140 REKRLAKEARFQETQAKNAALGKKVKEAPAARG-KGRGEAAFFRVTCKDNGRGMPHDDIP 199

Query: 183 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDID 242
           NM GRVLSGTKYGL+QTRGKFGLGAKMALIWSKMSTGLPIEI SSMKGQN+ SFCRLDID
Sbjct: 200 NMLGRVLSGTKYGLRQTRGKFGLGAKMALIWSKMSTGLPIEIKSSMKGQNFISFCRLDID 259

Query: 243 IHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKF 302
           IH+N+PH+H+ EKR+NKD WHGAE+QV+IEGNWTT+RSKILHYMRQMAVITPYAQFLF+F
Sbjct: 260 IHKNVPHVHLHEKRENKDRWHGAELQVIIEGNWTTHRSKILHYMRQMAVITPYAQFLFRF 319

Query: 303 VSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFV 362
           +SD+PDKN+TI+F+RRTDVMPP+PL+TKHHPSAVDLLLIKRLI+ET KQNLLQFLQHEFV
Sbjct: 320 LSDSPDKNLTIQFARRTDVMPPIPLQTKHHPSAVDLLLIKRLISETTKQNLLQFLQHEFV 379

Query: 363 NIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNL 422
           NI KS AERLIGEMGP+FS K +VKSLTSQQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNL
Sbjct: 380 NISKSHAERLIGEMGPDFSAKTTVKSLTSQQLVRIHQLFRQAKFDDPSGNCLSPAGEYNL 439

Query: 423 RLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQ 482
           RLGIIKELHPD+VAT++ S QVFEGHPFIVEAG+SIGGKDVK GLNIFR+ANRIPLLFEQ
Sbjct: 440 RLGIIKELHPDLVATHASSPQVFEGHPFIVEAGISIGGKDVKHGLNIFRYANRIPLLFEQ 499

Query: 483 GADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVK 542
           GADV+TRTALKRINW+SYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIGDDI+EIASAV+
Sbjct: 500 GADVITRTALKRINWSSYKINQQQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIASAVQ 559

Query: 543 SAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHD 602
           SA++QCC+QLKSKIVKK+QARE+Q+RKRNL+RYIPD   AI   L E+A     K+ R+D
Sbjct: 560 SALKQCCLQLKSKIVKKLQARERQDRKRNLNRYIPDVARAIMETLGEIADESPPKRPRYD 619

Query: 603 CEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDA 662
            ED E+L KV++  +T  T RD L +HVEQVD+EMA+EYA QSG S EP+E +Y+ SL+ 
Sbjct: 620 KEDEELLEKVNSEEVTEMTFRDCLTQHVEQVDYEMALEYAMQSGVSEEPREALYLNSLEG 679

Query: 663 TDNFTDFQSPLFVFRLI 673
           +  F DFQSP+FVFR I
Sbjct: 680 SYKFIDFQSPVFVFRFI 695

BLAST of IVF0021483 vs. ExPASy Swiss-Prot
Match: Q6H442 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 1.0e-303
Identity = 523/677 (77.25%), Postives = 596/677 (88.04%), Query Frame = 0

Query: 3   AGGGSESPDEPKKGKSKTPRK-------PKESILKQKSPAEFFAENKNIAGFDNPGKCLY 62
           A   S +      GK K   K        + S+LKQKSPAEFFAENKNIAGFDNPGK LY
Sbjct: 20  AASSSAAAKGKAAGKGKAASKASALATAKESSLLKQKSPAEFFAENKNIAGFDNPGKSLY 79

Query: 63  TTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKA 122
           TT+RELVEN+LDSAESISELP +EI IEEI K+KFN+MIGLVDR+R+DEELYDD+E+AKA
Sbjct: 80  TTMRELVENALDSAESISELPDIEIIIEEITKSKFNTMIGLVDRQRIDEELYDDFESAKA 139

Query: 123 REKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIP 182
           REKRLAKEAR QE QAKNAALGKK +E  A++G KGRGEA+F+RVTCKDNG+GMPHDDIP
Sbjct: 140 REKRLAKEARFQETQAKNAALGKKVKEAPAARG-KGRGEAAFFRVTCKDNGRGMPHDDIP 199

Query: 183 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDID 242
           NM GRVLSGTKYGL+QTRGKFGLGAKMALIWSKMSTGLPIEI SSMKGQN+ SFC LDID
Sbjct: 200 NMLGRVLSGTKYGLRQTRGKFGLGAKMALIWSKMSTGLPIEIKSSMKGQNFISFCLLDID 259

Query: 243 IHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKF 302
           IH+N+PH+H+ EKR+NKD WHGAE+QV+IEGNWTT+RSKILHYMRQMAVITPYAQFLF+F
Sbjct: 260 IHKNVPHVHLHEKRENKDRWHGAELQVIIEGNWTTHRSKILHYMRQMAVITPYAQFLFRF 319

Query: 303 VSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFV 362
           +SD+PDKN+TI+F+RRTDVMPP+PL+TKHHPSAVDLLLIKRLI+ET KQNLLQFLQHEFV
Sbjct: 320 LSDSPDKNLTIQFARRTDVMPPIPLQTKHHPSAVDLLLIKRLISETTKQNLLQFLQHEFV 379

Query: 363 NIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNL 422
           NI KS AERLIGEMGP+FS K +VKSLTSQQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNL
Sbjct: 380 NISKSHAERLIGEMGPDFSAKTTVKSLTSQQLVRIHQLFRQAKFDDPSGNCLSPAGEYNL 439

Query: 423 RLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQ 482
           RLGIIKELHPD+VAT++ S QVFEGHPFIVEAG+SIGGKDVK GLNIFR+ANRIPLLFEQ
Sbjct: 440 RLGIIKELHPDLVATHASSPQVFEGHPFIVEAGISIGGKDVKHGLNIFRYANRIPLLFEQ 499

Query: 483 GADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVK 542
           GADV+TRTALKRINW+SYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIGDDI+EIASAV+
Sbjct: 500 GADVITRTALKRINWSSYKINQQQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIASAVQ 559

Query: 543 SAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHD 602
           SA++QCC+QLKSKIVKK+QARE+Q+RKRNL+RYIPD   AI   L E+A     K+ R+D
Sbjct: 560 SALKQCCLQLKSKIVKKLQARERQDRKRNLNRYIPDVARAIMETLGEIADESPPKRPRYD 619

Query: 603 CEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDA 662
            ED E+L KV++  +T  T RD L +HVEQVD+EMA+EYA QSG S EP+E +Y+ SL+ 
Sbjct: 620 KEDEELLEKVNSEEVTEMTFRDCLTQHVEQVDYEMALEYAMQSGVSEEPREALYLNSLEG 679

Query: 663 TDNFTDFQSPLFVFRLI 673
           +  F DFQSP+FVFR I
Sbjct: 680 SYKFIDFQSPVFVFRFI 695

BLAST of IVF0021483 vs. ExPASy Swiss-Prot
Match: Q9YE64 (Type 2 DNA topoisomerase 6 subunit B OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=top6B PE=3 SV=2)

HSP 1 Score: 308.9 bits (790), Expect = 1.4e-82
Identity = 188/584 (32.19%), Postives = 302/584 (51.71%), Query Frame = 0

Query: 30  KQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTK 89
           ++ S AEFFA+NK +AGF NP + LY T+RELVENSLD+ ++   LP + I+I +I    
Sbjct: 29  REMSVAEFFAKNKELAGFANPTRALYQTIRELVENSLDATDAKGILPWIHISIRQI---- 88

Query: 90  FNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGM 149
                                                                    +G 
Sbjct: 89  ---------------------------------------------------------EGS 148

Query: 150 KGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKM 209
           +GRG    Y VT +DNG G+P   +   FG+VL  +KY ++QTRG +GLG K A+++ +M
Sbjct: 149 EGRGGRPRYTVTVEDNGIGVPVTSMAMAFGKVLFSSKYVIRQTRGMYGLGVKAAILYGQM 208

Query: 210 STGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWT 269
           + G P+E+ S+ KG  Y    +L ID+ RN P I  + + + +  WHG  + + +EG+W+
Sbjct: 209 TAGTPVEVYSATKGSQYVYMMKLVIDVQRNEPRILERGEWNKRGSWHGTRVTLSLEGDWS 268

Query: 270 TYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAV 329
             +SKIL Y+++ AVI PYA+ +     +TPD  I   F R T  +P  P E+K HP  V
Sbjct: 269 RAKSKILEYIKRTAVIAPYAEIIL----ETPDGEI-YYFPRSTRKLPKPPRESKPHPHGV 328

Query: 330 DLLLIKRLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMG--PEFSPKMSVKSLTSQQI 389
           D+  +K ++  T    L +FL  EF +IG   A+  + + G  P+  P+  +K    + +
Sbjct: 329 DIEQLKSMLRSTRASTLREFLVKEFQSIGDKTAQDFLEKYGLDPDLKPRELLKKGRDKLL 388

Query: 390 VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEA 449
            R+     +  F  P  D LSP G   + +G+ +  +P+ V   S S + + GHPFIVE 
Sbjct: 389 RRLADALMEYPFRAPKSDYLSPIGSDIIEIGLRRMFNPEWVGAVSRSPKAYRGHPFIVEV 448

Query: 450 GVSIGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFV 509
           G++ GG  + +    + R+AN+IPLL+E+  D V+   +  INW  Y ++     + V V
Sbjct: 449 GIAYGGGIEARSEPLLLRYANKIPLLYEERED-VSYKVVSSINWRQYNVDFPAPLV-VLV 508

Query: 510 SIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLS 569
            I STK+P+KG GKE +  D+ EI + +++A+Q+   +L+  + +K +  E   R   L+
Sbjct: 509 HIASTKVPYKGVGKESV-SDVPEIEAEIRNAVQEVARRLRLYLSRKAREEEAIRRSVTLA 542

Query: 570 RYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLI 611
           +YIP+   ++   L+  ++    K +    +  E L K+ AR I
Sbjct: 569 KYIPEVAVSLAYFLRPPSKWQPPKPEEVK-KIQEALIKIVARHI 542

BLAST of IVF0021483 vs. ExPASy Swiss-Prot
Match: C3NHT6 (Type 2 DNA topoisomerase 6 subunit B OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) OX=419942 GN=top6B PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.5e-79
Identity = 199/603 (33.00%), Postives = 310/603 (51.41%), Query Frame = 0

Query: 33  SPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNS 92
           SPAEFF  N  +AGF NP + LY TVREL+ENSLD+ +    LP ++ITI+         
Sbjct: 11  SPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITID--------- 70

Query: 93  MIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGR 152
              L+D  R                                                   
Sbjct: 71  ---LIDEAR--------------------------------------------------- 130

Query: 153 GEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTG 212
                Y+V   DNG G+P  ++PN FGRVL  +KY  +QTRG +GLG K A+++S+M   
Sbjct: 131 ---QIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYANRQTRGMYGLGVKAAVLYSQMHQD 190

Query: 213 LPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272
            PIEI +S          +L IDI++N P I  +   +N   +HG  + + I G+W   +
Sbjct: 191 KPIEIETSPANSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAK 250

Query: 273 SKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLL 332
           S+I  Y+++  +ITPYA+F+FK     P+ N+T  + R T+ +P  P E K HP  VD  
Sbjct: 251 SRIYEYIKRTYIITPYAEFIFK----DPEGNVTY-YPRLTNKIPKPPQEVKPHPYGVDRE 310

Query: 333 LIKRLIAETAKQ-NLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIH 392
            IK LI    +   + +FL +EF +IG + A++++   G    P   VK+LT ++I R+ 
Sbjct: 311 EIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAG--LKPNKKVKNLTEEEITRLV 370

Query: 393 QLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVS 452
           + F++ + F  PS D LS  GE  + LG+ K  +PD  A+ +   + ++GHPFIVEAGV+
Sbjct: 371 ETFKKYEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVA 430

Query: 453 IGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIV 512
            GG   V +   + R+AN+IPL++++ +DV+ +  ++ ++W  Y I   Q ++ V V + 
Sbjct: 431 FGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWK-VVEELDWKRYGIESDQYQMVVMVHLC 490

Query: 513 STKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNL---S 572
           STKIP+K  GKE I  ++ +I   +K+A+ +   +LK  + +K   R++QE K+ L    
Sbjct: 491 STKIPYKSAGKESIA-EVEDIEKEIKNALMEVARKLKQYLSEK---RKEQEAKKKLLAYL 530

Query: 573 RYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQV 630
           +YIP+ + ++   L    +   SK +    E    L      LI  E  R      V +V
Sbjct: 551 KYIPEVSRSLATFLASGNKELVSKYQNEISEGLFKLISKKLDLINIEEYR-----KVYRV 530

BLAST of IVF0021483 vs. ExPASy TrEMBL
Match: A0A5A7TIV7 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 674
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of IVF0021483 vs. ExPASy TrEMBL
Match: A0A1S3BM58 (DNA topoisomerase 6 subunit B OS=Cucumis melo OX=3656 GN=LOC103491545 PE=3 SV=1)

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 674
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of IVF0021483 vs. ExPASy TrEMBL
Match: A0A5D3DDA2 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 666/691 (96.38%), Postives = 666/691 (96.38%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN----------- 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660
           IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 667
           TQSGASAEPKEEMYIQSLDATDNFTDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of IVF0021483 vs. ExPASy TrEMBL
Match: A0A6J1DJZ7 (DNA topoisomerase 6 subunit B OS=Momordica charantia OX=3673 GN=LOC111021237 PE=3 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 646/672 (96.13%), Postives = 663/672 (98.66%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTP+KPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPKKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRER+D  LYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERIDAALYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKK REPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKVREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEK DNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQF+FKFVSDTP
Sbjct: 241 PHIHIQEKCDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFIFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAET KQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETPKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGI+
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIV 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KEL P+MVATYSGSAQVFEGHPFIVEAGVS+GGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELQPEMVATYSGSAQVFEGHPFIVEAGVSVGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYK+NQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKMNQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKRH+ EDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHESEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAEP+EE+YIQSL+ATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPREEIYIQSLEATDNFT 660

Query: 661 DFQSPLFVFRLI 673
           DFQSPLFVFRL+
Sbjct: 661 DFQSPLFVFRLV 672

BLAST of IVF0021483 vs. ExPASy TrEMBL
Match: A0A6J1H1C2 (DNA topoisomerase 6 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111459466 PE=3 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 647/672 (96.28%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTC+DNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PH+H+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSAR+ITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEE+YIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLI 673
           DF+SPLFVFRL+
Sbjct: 661 DFKSPLFVFRLV 672

BLAST of IVF0021483 vs. NCBI nr
Match: XP_008449755.1 (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo] >KAA0041365.1 DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1308 bits (3384), Expect = 0.0
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 673
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of IVF0021483 vs. NCBI nr
Match: XP_011653575.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis sativus] >KAE8649491.1 hypothetical protein Csa_018197 [Cucumis sativus])

HSP 1 Score: 1293 bits (3347), Expect = 0.0
Identity = 665/673 (98.81%), Postives = 669/673 (99.41%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQAQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEEMYIQSLDAT +FT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHSFT 660

Query: 661 DFQSPLFVFRLIC 673
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of IVF0021483 vs. NCBI nr
Match: XP_038902757.1 (DNA topoisomerase 6 subunit B isoform X1 [Benincasa hispida])

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 661/672 (98.36%), Postives = 669/672 (99.55%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           M+AGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MDAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKD WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDQWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQ HASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQAHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAEPKEE+YIQSLDATD+FT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATDSFT 660

Query: 661 DFQSPLFVFRLI 672
           DF+SPLFVFRLI
Sbjct: 661 DFRSPLFVFRLI 672

BLAST of IVF0021483 vs. NCBI nr
Match: TYK21651.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1280 bits (3311), Expect = 0.0
Identity = 666/691 (96.38%), Postives = 666/691 (96.38%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN----------- 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660
           IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 666
           TQSGASAEPKEEMYIQSLDATDNFTDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of IVF0021483 vs. NCBI nr
Match: XP_023532908.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1266 bits (3275), Expect = 0.0
Identity = 650/672 (96.73%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIH+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660
           ILSKVSAR+ITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEE+YIQSLDATDNFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLI 672
           DF+SPLFVFRL+
Sbjct: 661 DFKSPLFVFRLV 672

BLAST of IVF0021483 vs. TAIR 10
Match: AT3G20780.1 (topoisomerase 6 subunit B )

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 547/662 (82.63%), Postives = 605/662 (91.39%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT A+Y VLK+M + H +K+KR+  ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDAT-DNFTDFQSPLFVFR 671
            ETL++KLAEHVEQVD+EMA+EYATQSG S EP+E +Y+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LI 673
           L+
Sbjct: 668 LM 669