IVF0020273 (gene) Melon (IVF77) v1

BLAST of IVF0020273 vs. ExPASy Swiss-Prot
Match: P81898 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Prunus dulcis OX=3755 PE=1 SV=2)

HSP 1 Score: 753.4 bits (1944), Expect = 2.0e-216
Identity = 375/560 (66.96%), Postives = 436/560 (77.86%), Query Frame = 0

Query: 47  HSTPPTVFFEVTKPIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIV 106
           H TPPTVFFEVTKPI  PK KPCS L+L HDFAYTYG+ PV A+YTPPS C    FS IV
Sbjct: 6   HDTPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIV 65

Query: 107 LEWKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLA 166
           LEWKATCR RQFDRIFGVWLGGVEILRSCTAEPR  GIVWTV+KDITRY SLL  NQTLA
Sbjct: 66  LEWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLA 125

Query: 167 VYLGNLVDKTYTGIYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNL 226
           VYLGNL+DKTYTGIY+V I +HFYP +E         + L S Y   S ADLILPISRNL
Sbjct: 126 VYLGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGY--HSWADLILPISRNL 185

Query: 227 PLNDGLWFEVQNSTDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLT 286
           PLNDGLWFEVQNS D +LKEF+IPQN YRA+LEVYVSFHENDEFWYSNLPN+YI ANNL+
Sbjct: 186 PLNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLS 245

Query: 287 DTPGNGPFREVLVDLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPF 346
            TPGNGPFREV+V LDG++V AVWPFTVI+TGG+NPLLWRPI+ IGSF+LP+YDIE+TPF
Sbjct: 246 GTPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPF 305

Query: 347 LGNLLDGKGHSFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNF 406
           LG +LDGK H FGF VT+ALNVW+++ANLHLWLD  S KTE KL  H      +S   +F
Sbjct: 306 LGKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDF 365

Query: 407 TGADGTFLTKVTRSVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFN 466
            G +GTFLT+ +RSVSS GWVKSS+G ITT S QD  YSN M++G +G+ Q VNQ+I FN
Sbjct: 366 KGLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKIIFN 425

Query: 467 SSVYAKQESSHVYSSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFIS 526
            SVY    SS+V+S  + KTFPLY+Y++ + QGNG+Y  I N+ LGF EKK+ GLG   S
Sbjct: 426 DSVYINLPSSYVHSLTSHKTFPLYLYTDFLGQGNGTYLLITNVDLGFIEKKS-GLGFSNS 485

Query: 527 SLKNVQNGQGLMVVKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCG 586
           SL+N+++ +G MVVK NLV SGL STQQ YRY     CY R+ISSSNYTIL+D V + C 
Sbjct: 486 SLRNLRSAEGNMVVKNNLVVSGLESTQQIYRYDGGKFCYFRNISSSNYTILYDKVGSKCN 545

Query: 587 EKQNSS-ESLRFRRWPIPAR 606
           +K  S+ + +  R WP  AR
Sbjct: 546 KKSLSNLDFVLSRLWPFGAR 562

BLAST of IVF0020273 vs. ExPASy TrEMBL
Match: A0A5D3DX64 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001000 PE=4 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60
           MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP
Sbjct: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60

Query: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120
           IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120

Query: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180

Query: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240
           YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240

Query: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300
           DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD
Sbjct: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300

Query: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360
           LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF
Sbjct: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360

Query: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS
Sbjct: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420

Query: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480
           VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS
Sbjct: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480

Query: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540
           SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV
Sbjct: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540

Query: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600
           KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW
Sbjct: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600

Query: 601 PIPARRASLDSNLFVNNGHV 621
           PIPARRASLDSNLFVNNGHV
Sbjct: 601 PIPARRASLDSNLFVNNGHV 620

BLAST of IVF0020273 vs. ExPASy TrEMBL
Match: A0A1S3CJY5 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Cucumis melo OX=3656 GN=LOC103501812 PE=4 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60
           MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP
Sbjct: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60

Query: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120
           IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120

Query: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180

Query: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240
           YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240

Query: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300
           DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD
Sbjct: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300

Query: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360
           LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF
Sbjct: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360

Query: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS
Sbjct: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420

Query: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480
           VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS
Sbjct: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480

Query: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540
           SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV
Sbjct: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540

Query: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600
           KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW
Sbjct: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600

Query: 601 PIPARRASLDSNLFVNNGHV 621
           PIPARRASLDSNLFVNNGHV
Sbjct: 601 PIPARRASLDSNLFVNNGHV 620

BLAST of IVF0020273 vs. ExPASy TrEMBL
Match: A0A0A0L222 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G627190 PE=4 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 3   SSSFLLLIS---LLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 62
           +SSFLLLIS   LLLLLLPFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+K
Sbjct: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61

Query: 63  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 122
           PIVTPK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD
Sbjct: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121

Query: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 182
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181

Query: 183 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 242
           IYNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS
Sbjct: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 241

Query: 243 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 302
           TDV+ KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV
Sbjct: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 303 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 362
           DLDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FG
Sbjct: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361

Query: 363 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 422
           FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTR
Sbjct: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421

Query: 423 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 482
           SVSS GWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVY
Sbjct: 422 SVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481

Query: 483 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 542
           S K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+V
Sbjct: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541

Query: 543 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 602
           VKG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRR
Sbjct: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601

Query: 603 WPIPARRASLDSNLFVNNGHV 621
           WPIPARRA LDSNLFVNNG V
Sbjct: 602 WPIPARRAFLDSNLFVNNGRV 622

BLAST of IVF0020273 vs. ExPASy TrEMBL
Match: A0A6J1CHN7 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Momordica charantia OX=3673 GN=LOC111011511 PE=4 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 6.0e-293
Identity = 506/622 (81.35%), Postives = 550/622 (88.42%), Query Frame = 0

Query: 1   MASS-SFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 60
           MASS S  L ISLLLLLLPF+SDA+LHKTR R+ DL SE   +    H+ PPT+FFEVTK
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI TPKAKPCS+L L HDF YTYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYNVKIHIHFYPEEEGFGGN-GVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           IY+V+IHIHFYPEE+ FG N G + EK  S YDS   ADLI+PISR+LPLNDGLWFEVQ+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQS 240

Query: 241 STDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVL 300
           STDVQ+KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVL
Sbjct: 241 STDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSF 360
           V LDG IV AVWPFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK H+F
Sbjct: 301 VSLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVT 420
           GFRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVS    S++ NFTG DGTF TKVT
Sbjct: 361 GFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVT 420

Query: 421 RSVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHV 480
           RSVSS GWVKSS G ITT SNQD SYSN M +G+NGSSQ VNQ+I FNSSVYAKQ+SSHV
Sbjct: 421 RSVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHV 480

Query: 481 YSSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLM 540
           YS K+LKTFPLY+YS+T D+GNG+Y+S+ANLTLGFNEKKTDG GSFISSLKN+QNGQGLM
Sbjct: 481 YSFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLM 540

Query: 541 VVKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFR 600
           VVK +LVT GLGSTQQDYRY+DD  CY R+ISSSNYTIL DNVKN CG+K  S  SLRF 
Sbjct: 541 VVKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFG 600

Query: 601 RWPIPARRASLDSNLFVNNGHV 621
           RWPIPARRA LDS+L VN+ HV
Sbjct: 601 RWPIPARRAFLDSSLQVNSVHV 620

BLAST of IVF0020273 vs. ExPASy TrEMBL
Match: A0A6J1FLZ6 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita moschata OX=3662 GN=LOC111446428 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.1e-289
Identity = 492/610 (80.66%), Postives = 548/610 (89.84%), Query Frame = 0

Query: 1   MASS-SFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 60
           MASS S  LLISLL +LLPFHS+ADLH+T LR+ DLFSES ++ T +H+TPPT+FFEVTK
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI TP+A+PCSLL+L HDF YTYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 240
           IY+VKIHIHFYPEE+ F     YSEKL + Y  DSRADLILPISR+LPLNDGLWFEVQNS
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLAAGY--DSRADLILPISRSLPLNDGLWFEVQNS 240

Query: 241 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 300
           TD+Q+KEFEIPQN YRA+LEVY+SFHENDEFWYSNLPNDYI+ANNL+ TPGNGPFREVLV
Sbjct: 241 TDIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLV 300

Query: 301 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 360
           +LD +IV A+WPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H FG
Sbjct: 301 NLDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFG 360

Query: 361 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 420
           F VTHALNVW+INANLHLWLDD S KTE KLLNHI+S  S S+DLNFTG DGTFLTK +R
Sbjct: 361 FSVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASR 420

Query: 421 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 480
           SVSS+GWVKSS G ITT SNQD SYSN M+MGN+G+SQ VNQEI FNSSVYAK+ES HVY
Sbjct: 421 SVSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVY 480

Query: 481 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 540
           S K+LKTFPLYMYSNTID+GNGS++SIANLTLGFNEKK DGLGSFISSLKNVQNGQG+M 
Sbjct: 481 SFKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMT 540

Query: 541 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 600
           VKG+LVT GLGSTQQDYRY+DD  CYSR+ISSSNYTIL+D+V ++C +K  S ESLRFR+
Sbjct: 541 VKGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRK 600

Query: 601 WPIPARRASL 610
           WP  +RR  L
Sbjct: 601 WPFLSRRVFL 604

BLAST of IVF0020273 vs. NCBI nr
Match: XP_008463736.1 (PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo] >KAA0066768.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa] >TYK27915.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa])

HSP 1 Score: 1253 bits (3242), Expect = 0.0
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60
           MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP
Sbjct: 1   MASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 60

Query: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120
           IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 61  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 120

Query: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 121 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 180

Query: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240
           YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 181 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 240

Query: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300
           DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD
Sbjct: 241 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 300

Query: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360
           LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF
Sbjct: 301 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 360

Query: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS
Sbjct: 361 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 420

Query: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480
           VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS
Sbjct: 421 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 480

Query: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540
           SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV
Sbjct: 481 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 540

Query: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600
           KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW
Sbjct: 541 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 600

Query: 601 PIPARRASLDSNLFVNNGHV 620
           PIPARRASLDSNLFVNNGHV
Sbjct: 601 PIPARRASLDSNLFVNNGHV 620

BLAST of IVF0020273 vs. NCBI nr
Match: XP_004146092.2 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis sativus] >KGN55069.1 hypothetical protein Csa_011983 [Cucumis sativus])

HSP 1 Score: 1159 bits (2998), Expect = 0.0
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 3   SSSFLLLISLLLLLL---PFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 62
           +SSFLLLISLLLLLL   PFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+K
Sbjct: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61

Query: 63  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 122
           PIVTPK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD
Sbjct: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121

Query: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 182
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181

Query: 183 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 242
           IYNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS
Sbjct: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 241

Query: 243 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 302
           TDV+ KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV
Sbjct: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 303 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 362
           DLDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FG
Sbjct: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361

Query: 363 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 422
           FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTR
Sbjct: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421

Query: 423 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 482
           SVSS GWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVY
Sbjct: 422 SVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481

Query: 483 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 542
           S K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+V
Sbjct: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541

Query: 543 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 602
           VKG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRR
Sbjct: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601

Query: 603 WPIPARRASLDSNLFVNNGHV 620
           WPIPARRA LDSNLFVNNG V
Sbjct: 602 WPIPARRAFLDSNLFVNNGRV 622

BLAST of IVF0020273 vs. NCBI nr
Match: XP_038899804.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Benincasa hispida])

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 527/618 (85.28%), Postives = 562/618 (90.94%), Query Frame = 0

Query: 3   SSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPIV 62
           SS  +LLISL+ LLLPFHS+A+LHK RLR SDL S+S ++TTAD++TPPTVFFEVTKPI 
Sbjct: 12  SSLLVLLISLIFLLLPFHSNANLHKIRLRHSDLLSDSGLTTTADYTTPPTVFFEVTKPIE 71

Query: 63  TPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIF 122
           TPKAKPCSLLVL HDFA+TYGK PVLADYTPPSHCSFTKFSKIVLEW ATC+GRQFDRIF
Sbjct: 72  TPKAKPCSLLVLQHDFAFTYGKSPVLADYTPPSHCSFTKFSKIVLEWTATCQGRQFDRIF 131

Query: 123 GVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGIYN 182
           GVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTGIY+
Sbjct: 132 GVWLGGVEILRSCTAEPRATGIVWTVQKDITRYDSLLSKNQTLAVYLGNLVDKTYTGIYH 191

Query: 183 VKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV 242
           VKIH+HFYPE +     GV SEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV
Sbjct: 192 VKIHVHFYPEAD----TGVSSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV 251

Query: 243 QLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDLD 302
           Q KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV LD
Sbjct: 252 QSKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVSLD 311

Query: 303 GDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFRV 362
           G+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLG+LLDGK H+ GF V
Sbjct: 312 GEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGHLLDGKVHNCGFSV 371

Query: 363 THALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSVS 422
           THALNVW+INANLHLWLDDNS KTE KLL+HIVS P +S DLNFTG DGTFLTKVTRSVS
Sbjct: 372 THALNVWYINANLHLWLDDNSAKTEGKLLDHIVSPPFISVDLNFTGPDGTFLTKVTRSVS 431

Query: 423 SAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSSK 482
           S GWVKSSFG I T SNQDLSYSN M+MGNNGSSQTV QEI FN SVYAKQESSHVYS K
Sbjct: 432 STGWVKSSFGIINTQSNQDLSYSNSMVMGNNGSSQTVTQEIQFNGSVYAKQESSHVYSFK 491

Query: 483 TLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVKG 542
           +LKTFPLYMYSNTID+GNG+Y+SIANLTLGFNEKKTDG GSF SSLKN+QNGQGLMVVKG
Sbjct: 492 SLKTFPLYMYSNTIDEGNGTYQSIANLTLGFNEKKTDGFGSFTSSLKNLQNGQGLMVVKG 551

Query: 543 NLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRWPI 602
           +LVTSGLGSTQQDYRY+DD+QCYSR+ISSSNYTIL+DNVK+ CG K      L FR   I
Sbjct: 552 HLVTSGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKDFCGRKTRILRKLGFRGGSI 611

Query: 603 PARRASLDSNLFVNNGHV 620
           PARRA LDSNL VNN HV
Sbjct: 612 PARRAFLDSNLHVNNSHV 625

BLAST of IVF0020273 vs. NCBI nr
Match: XP_022140996.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Momordica charantia])

HSP 1 Score: 1013 bits (2619), Expect = 0.0
Identity = 506/622 (81.35%), Postives = 550/622 (88.42%), Query Frame = 0

Query: 1   MASS-SFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 60
           MASS S  L ISLLLLLLPF+SDA+LHKTR R+ DL SE   +    H+ PPT+FFEVTK
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI TPKAKPCS+L L HDF YTYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYNVKIHIHFYPEEEGFGGNGV-YSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           IY+V+IHIHFYPEE+ FG NG  + EK  S YDS   ADLI+PISR+LPLNDGLWFEVQ+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQS 240

Query: 241 STDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVL 300
           STDVQ+KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVL
Sbjct: 241 STDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSF 360
           V LDG IV AVWPFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK H+F
Sbjct: 301 VSLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVT 420
           GFRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVS    S++ NFTG DGTF TKVT
Sbjct: 361 GFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVT 420

Query: 421 RSVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHV 480
           RSVSS GWVKSS G ITT SNQD SYSN M +G+NGSSQ VNQ+I FNSSVYAKQ+SSHV
Sbjct: 421 RSVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHV 480

Query: 481 YSSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLM 540
           YS K+LKTFPLY+YS+T D+GNG+Y+S+ANLTLGFNEKKTDG GSFISSLKN+QNGQGLM
Sbjct: 481 YSFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLM 540

Query: 541 VVKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFR 600
           VVK +LVT GLGSTQQDYRY+DD  CY R+ISSSNYTIL DNVKN CG+K  S  SLRF 
Sbjct: 541 VVKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFG 600

Query: 601 RWPIPARRASLDSNLFVNNGHV 620
           RWPIPARRA LDS+L VN+ HV
Sbjct: 601 RWPIPARRAFLDSSLQVNSVHV 620

BLAST of IVF0020273 vs. NCBI nr
Match: XP_023525341.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 494/606 (81.52%), Postives = 546/606 (90.10%), Query Frame = 0

Query: 4   SSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPIVT 63
           SS LLLISLL +LLPF SDADLH+ RLR+ DLFSES ++ T DH+TPPT+FFEVTKPI T
Sbjct: 6   SSPLLLISLLFILLPFLSDADLHRARLRRPDLFSESELAATGDHTTPPTLFFEVTKPIET 65

Query: 64  PKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFG 123
           P+A+PCSLL+L HDF YTYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFDRIFG
Sbjct: 66  PEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDRIFG 125

Query: 124 VWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGIYNV 183
           VWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTGIY+V
Sbjct: 126 VWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGIYHV 185

Query: 184 KIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDVQ 243
           KIHIHFYPEE+ F     YSEKL + YDS  RADLILPISR+LPLNDGLWFEVQNSTD+Q
Sbjct: 186 KIHIHFYPEEKRF----QYSEKLAAGYDS--RADLILPISRSLPLNDGLWFEVQNSTDIQ 245

Query: 244 LKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDLDG 303
           +KEFEIPQN YRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLT TPGNGPFREVLV+LD 
Sbjct: 246 VKEFEIPQNSYRAVLEVYVSFHENDEFWYSNLPNDYILANNLTQTPGNGPFREVLVNLDD 305

Query: 304 DIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFRVT 363
           +IV A+WPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H FGF VT
Sbjct: 306 EIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKPHHFGFSVT 365

Query: 364 HALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSVSS 423
           HALNVW+INANLHLWLDD S KTE KLLNHI+S  S S+DLNFTG DGTFLTK +RSVSS
Sbjct: 366 HALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRSVSS 425

Query: 424 AGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSSKT 483
           +GWVKSS G ITT SNQD SYSN M+MGN+G+SQ VNQEI FNSSVYAK+ES HVYS K+
Sbjct: 426 SGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESDHVYSFKS 485

Query: 484 LKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVKGN 543
           LKTFPLYMYSNTID+GNGS++SIANLTLGFNEKK DGLGSFISSLKNVQNGQG+MVVKG+
Sbjct: 486 LKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKVDGLGSFISSLKNVQNGQGMMVVKGH 545

Query: 544 LVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRWPIP 603
           LVT GLGSTQQDYRY+DD  CYSR+ISSSNYTIL+D+V ++C +K  S ESLRFR+WP  
Sbjct: 546 LVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESHESLRFRKWPFL 605

Query: 604 ARRASL 609
           +RR  L
Sbjct: 606 SRRVFL 605

BLAST of IVF0020273 vs. TAIR 10
Match: AT3G14920.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 666.4 bits (1718), Expect = 2.3e-191
Identity = 336/577 (58.23%), Postives = 429/577 (74.35%), Query Frame = 0

Query: 23  ADLHKTRLR-QSDLFSESHVSTTADHST-PPTVFFEVTKPIV--TPKA-KPCSLLVLHHD 82
           +DLH+TR R +   FS    S+   + T  PT +FEV KP V   P A +PCS  +LHHD
Sbjct: 24  SDLHETRSRFKPPQFSPLFFSSLPQNVTKSPTRYFEVQKPPVPNLPTAQQPCSYQILHHD 83

Query: 83  FAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEILRSCTA 142
           F YTY KPPVL++YT PSHCS  +FSKIVLE+K+T +GRQFDRIFGVWL GVEILRSCTA
Sbjct: 84  FGYTYAKPPVLSNYTLPSHCSSREFSKIVLEFKSTSQGRQFDRIFGVWLDGVEILRSCTA 143

Query: 143 EPRATGIVWTVQKDITRYNSLLSKNQT--LAVYLGNLVDKTYTGIYNVKIHIHFYPEEEG 202
           EPR  GIVW+V+KD+T+Y+SLL KN+T  L+VYLGNL+DKTYTG+Y+V +  HFY  E  
Sbjct: 144 EPRPNGIVWSVEKDVTKYHSLLVKNETQILSVYLGNLIDKTYTGVYHVDVIFHFYQSESN 203

Query: 203 FGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDVQLKEFEIPQNVYR 262
                 Y         S S+AD+ILPISRNLPLNDGLWFE+ NS D + KEFEIP+NVYR
Sbjct: 204 LQDVSGY---------SSSKADMILPISRNLPLNDGLWFEIVNSNDTKYKEFEIPRNVYR 263

Query: 263 ALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDLDGDIVAAVWPFTVI 322
           A+LEVYVSFHENDEFWY NLPNDY+ ANNL+   GNGPFREV+V LDGDI  AVWPF V+
Sbjct: 264 AVLEVYVSFHENDEFWYGNLPNDYVTANNLS-VAGNGPFREVVVSLDGDIAGAVWPFPVV 323

Query: 323 YTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFRVTHALNVWFINANL 382
           +TGG+NPLLWRPI+ IGSF+LPSYDIE+TPFLG+LLDGK H  GF VT+ALNVW+I+ANL
Sbjct: 324 FTGGINPLLWRPITAIGSFDLPSYDIEITPFLGSLLDGKTHKVGFSVTNALNVWYIDANL 383

Query: 383 HLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSVSSAGWVKSSFGTIT 442
           HLWLD      E K+L+   SS  +S   +F G +G F TK  RS++S G VKSS G I 
Sbjct: 384 HLWLDQEKEIVEGKVLDFSRSSLEISSVSDFKGLNGNFTTKAKRSITSVGLVKSSHGDII 443

Query: 443 TLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSSKTLKTFPLYMYSNT 502
           T +NQ+ SY N M++G +G+ Q ++Q I  +  ++AK+ S  +Y++K++K+FP Y+ S+T
Sbjct: 444 TNANQEFSYENKMVLGKDGNLQIIDQLIQADDRIHAKRASREIYAAKSIKSFPFYLDSDT 503

Query: 503 IDQGNGSYRSIANLTLGFNEKKTDG----LGSFISSLKNVQNGQGLMVVKGNLVTSGLGS 562
           ++Q N +Y ++AN+++ FNE++++     + +F S L+N Q GQG+MVVK NLV SG GS
Sbjct: 504 LEQQNNTYLAVANVSMAFNEERSESDKGLMRTFKSKLENKQEGQGVMVVKNNLVVSGYGS 563

Query: 563 TQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEK 589
           TQQ Y Y    QCY R+ISS NYTIL+D V+++C +K
Sbjct: 564 TQQVYNYVGSDQCYFRNISSYNYTILYDKVESVCKKK 590

BLAST of IVF0020273 vs. TAIR 10
Match: AT5G05480.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.6e-107
Identity = 224/570 (39.30%), Postives = 319/570 (55.96%), Query Frame = 0

Query: 28  TRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPIVTPKAKP-CSLLVLHHDFAYTYGKPP 87
           +R    D F  S  ST       P  + E+ +P+ + +  P CS ++  H FA T  +PP
Sbjct: 24  SRPSSPDRFLRS-ASTCLRSPVEPQEYEELRRPLPSDQLTPSCSHVLFRHSFANTINRPP 83

Query: 88  VLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVW 147
               YTPPS C    +S +VL+ +A   G Q+DRI G+WLGGVE+LR+ TAEP  +GI W
Sbjct: 84  FTTPYTPPSSCISPPWSYVVLDLRAASSGDQYDRISGLWLGGVELLRTSTAEPSPSGIFW 143

Query: 148 TVQKDITRYNSLLSKNQ-TLAVYLGNLVDKTYTGIYNVKIHIHFYPEEEGFGGNGVYSEK 207
            V+KD++RY+SL  ++   + + L N+V+  YTGIY++ + + FY E      N   S +
Sbjct: 144 NVRKDVSRYSSLFMRSDLNVTMMLENIVNDVYTGIYHINVTLIFY-EFNPIASNLKISNR 203

Query: 208 LGSAYDSDSR----ADLILPISRNLPLNDGLWFEVQNSTDVQLKEFEIPQNVYRALLEVY 267
           LG   DS  R    ADLI+P+      N G WF ++N  +   K  +IP N  + +LE+Y
Sbjct: 204 LGFV-DSQKRDQTPADLIIPVCDG--GNRGFWFMIENPRETYSKGIQIPSNTRQIVLELY 263

Query: 268 VSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDLDGDIVAAVWPFTVIYTGGVN 327
           VSFH NDEFWYSN PN YI  NNLT   GNG +REV V +DG  V +  PF VI+TGG+N
Sbjct: 264 VSFHGNDEFWYSNPPNSYIRTNNLTTGRGNGAYREVFVKIDGRYVGSEVPFPVIFTGGIN 323

Query: 328 PLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFRVTHALNVWFINANLHLWLDD 387
           PL W P+  IG+FNLPSYD++LTPFLG LLDGK H F F V   ++ W ++ANLHLWLD 
Sbjct: 324 PLFWEPVVAIGAFNLPSYDMDLTPFLGLLLDGKSHEFAFGVNDGISYWLVDANLHLWLDH 383

Query: 388 NSVKTEAKLLNHIVSSPS--MSQDLNFTGADGTFLTKVTRSVSSAGWVKSSFGTITTLSN 447
            S   EA   + +  SPS  M +       DG+F  +   S    GWV+SS G +TT+  
Sbjct: 384 GSSNVEAG--SRLYDSPSRHMMRQEQLEQLDGSFKVEAEVSSEYDGWVRSSKGNLTTMVK 443

Query: 448 QDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSH-VYSSKTLKTFPLYMYSNT--- 507
                 +L+    NG+ + V Q +     V    ES   V      +T+PL + ++T   
Sbjct: 444 SMFKVDSLVRFEKNGTYKRVEQRVKTERIVEVTSESGKPVNRVVHQRTYPLTVITSTLRG 503

Query: 508 IDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVKGNLVTSGLGSTQQD 567
           +     +Y  + N++   NE  +D  G  +  + + Q+ +G M V+ + V +G  ST+Q 
Sbjct: 504 LTNDKDTYMLVTNVSHALNETHSD--GEALIQVYSRQDSEGWMEVEDHNVLAGEASTKQS 563

Query: 568 YRYYDDVQCYSRSISSSNYTILHDNVKNMC 586
             Y D+  CYSR+I ++N  I  D+  + C
Sbjct: 564 LSYIDEFSCYSRTIVAANGEIAQDSSSDSC 584