IVF0016565 (gene) Melon (IVF77) v1

Overview


Name	IVF0016565
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Target of rapamycin complex subunit LST8
Location	chr04: 28438859 .. 28441839 (+)
RNA-Seq Expression	IVF0016565
Synteny	IVF0016565

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCAACCATCAGTGATACTTGCAACTGCTAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACCATACAATATCCTGATTCTGTTAGTTCATAAATCCTTTCTGTTCTCTGTGTTTCTTGGTTATTCTGAAAGGAGCTTTGGTAGTATAACAAATTGAATAAATAAGAAAGTTATCACATTCTCGGAATATTATGTCATAACAAGTTACTATAAATCATGTTCCTTACCCATTGGTAGTTTACCTTAAATCTTGACTAACTAGGGTTGATGATGAATGTCTTTTACTCCTAATTATCATGAATTATGTGACAGCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAGTATGTAACCTGCTCTAACACCTTACTATTGTTATTTTAACTTTTCTTCATCTATTTTTCTTTTCTTCCAGAATCTTTTTAGATATAAGCTGAGTGAAAATAAATACTCCTTTCATTTTCTAATGCGTCTGTTCTTTCTACTTTTACTCCCAAACTACATGGCAGATAATGAGTTATGATTCACATACTAACAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACAGTAAAAATATGGGATTTAAGGTACATAAAGTGTTCAAAATATGGGACTTGATGTTGTTTAGACTTACATTCAAGGTTACCTGGACTTATTTCGAGAAATTCAGGGCTCCTGGTTGTCAGAGAGAGTATGAAAGTCGTGCTGCAGTTAACACCGTTGTTTTACATCCTAATCAGGTTATATATCACATTACCCCCTTTTTGCAGTTTCATATTATTTTACTTCAAAGCTTATTAGCCCTACTTGGTGGTGGAAATTTGTTCCTTGTCTCAATAATAATAGTACTGCGCAAGAAAATTCACTAGAGCCTGTCCTAAATGCAATATCCGTTTACTTTTTGGGCATGAAAAGGAATATTAATTTTACAATTTTCATTTAACCAATTCTGTCTTGCTTTCCTAGGAAAGATTCATCCTAGGGAAAATTAATAACCTTATCATCTTGCTTTTGCATTGTTAACCAAATTTCTCTCATTTGTAGACTGAACTGATATCTGGAGATCAAAATGGTAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTATGGCTTTGTGCATTTCATTTGTGTAATCGTAATTGAATGTGCCTCTGCCTTTTCATACTTTGTTTGAAAATAACTGTAGGTTGTCGTATTACTCTTTGTGGTGTCAGGCTAACACACTTTAAAAGAGGAAAAATTACTTGAATGAAATTAAGATCTTTTGGTGAAAAATATTAGTAACTATTCTCAACATTATTGCTTAAAGTTTGAGATAAAAAGAAAAAGAAGTGAAAGGGACAAGCTGTCCTCGAGACAGTTAAACTGTGGAGGAAAGAAGGAACATTTTTAAGAGGAAGGGGATTGAAGTTTAGAGATAGAGGAGGTTAAAGAAATCAAAGAGTTCAGAACAGTAGTTCTGTTATAATGCTTCTTCTAAATTTTATGTTGAAGGACCTGAACCTTTAATTTAACCTATTTGCTATCTCCTAAATGCGAGTTTGTACGTTATTTACTGCCAAAAAATGAACAGAAGTTGCCTAGTGGCATGGATTATCATGGTTACATCCCAATAATTTAGTGCGTTTCCAGGTGCCAGAAGTGGATACAGCTGTAAGATCTTTGACGGTTATGTGGGATGGGAGCTTGGTAGTTGCAGCAAATAATCATGGCACATGTTACGTCTGGCGCTTGTTACGTGGCACCCAGGTTGTACCTAATGCCAAATGCTGATTATCGTCTGCTGTACAATTCAAATAGGCTAACTCATTTTTCTCTTTCAGACGATGACAAACTTTGAGCCACTTCATAAACTTCAAGCACACAAGGGATACATTCTCAAGTGTCTTCTTTCACCTGAATTCTGTGAACCCCATCGGTCTTATTACTCAAAACTTCCTAAATTTTATTTTCTTTTATTTCTTCTGGTTGAACAATATATGTTGGAATAAATGATTGTTGTGACTTGGATATTTCTTCAAATTATAACATTCACGTGGCTTCACATCTTTTAGATACCTTGCCACTGCATCTTCTGACAGCACTGTCAAAATCTGGAACGTCGATGGATTCACATTAGAAAAGACCCTTATAGGTATCTGGCTCTCATTTATCTAGTGTTATTTCTGTCAAATTATCACTATACTGGATCTTCCTGGCAGTTACTGAGAAGTAAGTATAGTGCTTTACTTTGCAATTCCATGTGCTTTTCAGGACACCAACGCTGGGTGTGGGACTGTGTCTTCTCAGTTGATGGTGCATATCTTATAACAGGTGACTAAGTTATTGACAAAGCCTCAGTAGGCAATATTTTCTACCTAATTTTCTTCGAAAGCATTGATCTCTGTTGACCATTATGCAAACCATTATGCATTTTGGTGCAGTGGGTTAAGGGAGTGAGAAATATGTCTACATATCACGGATCTAGACCAAAGTAGCCATTTTTGTTAGATATTAAATAGCCTACAAGCTTGTAAAAAAGAAAAAGGTGTGGATATTCATAGTTATAAAGATAATAAACTTTAACTACAAGAATGACAATTAGAATGGATAGACTTCTGCTATTTTTTTAGTTGATTATTTCTATTTTGGCATTCTCATTGATGCATCCGTAAAACCTTTACTGTAAGTTTGCGGAAATAACTCAGGGTTTTGTGATTGTAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTTCACGATGGAGCCGAGCCATCGTCGGGTTGA

mRNA sequence

ATGACTCAACCATCAGTGATACTTGCAACTGCTAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACCATACAATATCCTGATTCTCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAATAATGAGTTATGATTCACATACTAACAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACAGTAAAAATATGGGATTTAAGGGCTCCTGGTTGTCAGAGAGAGTATGAAAGTCGTGCTGCAGTTAACACCGTTGTTTTACATCCTAATCAGACTGAACTGATATCTGGAGATCAAAATGGTAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTGCCAGAAGTGGATACAGCTGTAAGATCTTTGACGGTTATGTGGGATGGGAGCTTGGTAGTTGCAGCAAATAATCATGGCACATGTTACGTCTGGCGCTTGTTACGTGGCACCCAGACGATGACAAACTTTGAGCCACTTCATAAACTTCAAGCACACAAGGGATACATTCTCAAGTGTCTTCTTTCACCTGAATTCTGTGAACCCCATCGATACCTTGCCACTGCATCTTCTGACAGCACTGTCAAAATCTGGAACGTCGATGGATTCACATTAGAAAAGACCCTTATAGGACACCAACGCTGGGTGTGGGACTGTGTCTTCTCAGTTGATGGTGCATATCTTATAACAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTTCACGATGGAGCCGAGCCATCGTCGGGTTGA

Coding sequence (CDS)

ATGACTCAACCATCAGTGATACTTGCAACTGCTAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACCATACAATATCCTGATTCTCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAATAATGAGTTATGATTCACATACTAACAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACAGTAAAAATATGGGATTTAAGGGCTCCTGGTTGTCAGAGAGAGTATGAAAGTCGTGCTGCAGTTAACACCGTTGTTTTACATCCTAATCAGACTGAACTGATATCTGGAGATCAAAATGGTAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTGCCAGAAGTGGATACAGCTGTAAGATCTTTGACGGTTATGTGGGATGGGAGCTTGGTAGTTGCAGCAAATAATCATGGCACATGTTACGTCTGGCGCTTGTTACGTGGCACCCAGACGATGACAAACTTTGAGCCACTTCATAAACTTCAAGCACACAAGGGATACATTCTCAAGTGTCTTCTTTCACCTGAATTCTGTGAACCCCATCGATACCTTGCCACTGCATCTTCTGACAGCACTGTCAAAATCTGGAACGTCGATGGATTCACATTAGAAAAGACCCTTATAGGACACCAACGCTGGGTGTGGGACTGTGTCTTCTCAGTTGATGGTGCATATCTTATAACAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTTCACGATGGAGCCGAGCCATCGTCGGGTTGA

Protein sequence

MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATICCALHDGAEPSSG

Homology

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BLAST of IVF0016565 vs. ExPASy Swiss-Prot
Match: Q9AYE4 (Target of rapamycin complex subunit LST8 OS=Oryza sativa subsp. japonica OX=39947 GN=LST8 PE=1 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 3.3e-167
Identity = 268/312 (85.90%), Postives = 291/312 (93.27%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M QPSVILATASYDH+I+FWEAKSGRCYRT+Q+ +S +NRLEITPDKR+LAAAGNPHIRL
Sbjct: 1   MAQPSVILATASYDHSIKFWEAKSGRCYRTLQHTESHINRLEITPDKRFLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNS  P++SYDSHT+NVM+VGF CDGNWMYSGSEDGTV+IWDLR   CQREYESRA
Sbjct: 61  FDINSNSNHPVISYDSHTSNVMAVGFHCDGNWMYSGSEDGTVRIWDLRTATCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQ ELISGDQNGNIRVWDL ANSCSCELVPEVDTAVRSLTVMWDGS+VVAA
Sbjct: 121 AVNTVVLHPNQKELISGDQNGNIRVWDLAANSCSCELVPEVDTAVRSLTVMWDGSMVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NN GTCYVWRLL+GTQT+T FEPLHKLQAH GYILKCLLSPEFC+P+RYLATASSD TVK
Sbjct: 181 NNRGTCYVWRLLKGTQTITCFEPLHKLQAHDGYILKCLLSPEFCDPNRYLATASSDHTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGF LEKTL+GHQRWVWDCVFSVDGAYLITASSDTTARLW+MSTG+ ++VY  HHK
Sbjct: 241 IWNVDGFKLEKTLVGHQRWVWDCVFSVDGAYLITASSDTTARLWTMSTGEAIRVYTSHHK 300

Query: 301 ATICCALHDGAE 313
             +CCALHDGAE
Sbjct: 301 PVVCCALHDGAE 312

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BLAST of IVF0016565 vs. ExPASy Swiss-Prot
Match: Q9LV27 (Target of rapamycin complex subunit LST8-1 OS=Arabidopsis thaliana OX=3702 GN=LST8-1 PE=1 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.2e-164
Identity = 268/309 (86.73%), Postives = 285/309 (92.23%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDLRAPGCQ+EYES A
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDLRAPGCQKEYESVA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELVPEVDTAVRSLTVMWDG++VVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVPEVDTAVRSLTVMWDGTMVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NN GTCYVWRLLRG QTMT FEPLHKLQAH G+ILKCLLSP     ++YLATASSD TVK
Sbjct: 181 NNRGTCYVWRLLRGKQTMTEFEPLHKLQAHNGHILKCLLSP----ANKYLATASSDKTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGF LEK L GHQRWVWDCVFSVDG +L+TASSD TARLWSM  GKE+KVYQGHHK
Sbjct: 241 IWNVDGFKLEKVLTGHQRWVWDCVFSVDGEFLVTASSDMTARLWSMPAGKEVKVYQGHHK 300

Query: 301 ATICCALHD 310
           AT+CCALHD
Sbjct: 301 ATVCCALHD 305

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BLAST of IVF0016565 vs. ExPASy Swiss-Prot
Match: F4IIK6 (Non-functional target of rapamycin complex subunit LST8-2 OS=Arabidopsis thaliana OX=3702 GN=LST8-2 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.5e-127
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 6   VILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINS 65
           V LATAS+D TIR W+A++GRCY + +YPD  VNRLE+TP+K  L AA NPHIRLFD+ S
Sbjct: 11  VYLATASHDQTIRLWQARTGRCYFSFRYPDLHVNRLELTPEKGKLVAACNPHIRLFDLRS 70

Query: 66  NSPQ-PIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNT 125
            +P  P+ ++ SHT NVM+VGFQ  G+ MYSGSEDG+VKIWDLR   CQRE+ S + VNT
Sbjct: 71  YNPHIPVRNFVSHTKNVMAVGFQYTGHMMYSGSEDGSVKIWDLRVRECQREFRSVSPVNT 130

Query: 126 VVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNHG 185
           VVLHPNQTELISGDQNGNIRVWDL A+ CSCELVPEV T +RSLTVMWDG++VVAAN+ G
Sbjct: 131 VVLHPNQTELISGDQNGNIRVWDLRADLCSCELVPEVGTPIRSLTVMWDGTMVVAANDRG 190

Query: 186 TCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIWNV 245
           TCYVWR L   QTMT FEPLHKLQAH  +ILKCLLSP     +RYLATASSD TVKIWN+
Sbjct: 191 TCYVWRSLCERQTMTEFEPLHKLQAHNSHILKCLLSP---GNNRYLATASSDKTVKIWNL 250

Query: 246 DGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATIC 305
           DGF LEK L GH+RWVWDC FS+DG YL+TASSDTTARLWSM  GKE  VYQ H KAT+C
Sbjct: 251 DGFKLEKVLTGHERWVWDCDFSMDGEYLVTASSDTTARLWSMRAGKEEMVYQAHRKATVC 310

Query: 306 CAL 308
           C L
Sbjct: 311 CTL 310

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BLAST of IVF0016565 vs. ExPASy Swiss-Prot
Match: Q54D08 (Protein LST8 homolog OS=Dictyostelium discoideum OX=44689 GN=lst8 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.5e-108
Identity = 184/306 (60.13%), Postives = 222/306 (72.55%), Query Frame = 0


Query: 4   PSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDI 63
           P +ILATASYDHTI+FW+  SG CYR+I   +  +NRLEIT DK Y+AAAGNP  RLF++
Sbjct: 2   PGIILATASYDHTIKFWDPPSGGCYRSIDCGEFHINRLEITHDKLYIAAAGNPQTRLFEV 61

Query: 64  NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVN 123
           N+N+  P MS+D H  NV  VGFQ +G WMY+GSEDGTVKIWDL+APGCQR+YE  A VN
Sbjct: 62  NTNNNSPAMSFDGHKGNVTGVGFQKEGKWMYTGSEDGTVKIWDLKAPGCQRDYECSAPVN 121

Query: 124 TVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNH 183
           TVVLHPNQ ELISGDQNG+IRVWDL +N+CS ELVP+ +  + SLT+  DG LVVA+N  
Sbjct: 122 TVVLHPNQAELISGDQNGSIRVWDLISNTCSRELVPDGEVGITSLTISSDGGLVVASNTK 181

Query: 184 GTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIWN 243
           G C+VWRL  G    + FEPL K++AH   ILK L SP+     + LAT S+D TVKIWN
Sbjct: 182 GKCFVWRL--GEDDTSRFEPLQKIEAHNAPILKTLFSPD----TKLLATCSADHTVKIWN 241

Query: 244 VDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATI 303
              F + +TL GHQRWVWDC FS D AYL+T SSD  +RLW +  G  +K Y GH KA  
Sbjct: 242 TKKFNVVQTLNGHQRWVWDCAFSNDSAYLVTGSSDHLSRLWDLHQGDAVKTYSGHIKAVN 301

Query: 304 CCALHD 310
             AL+D
Sbjct: 302 AVALND 301

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BLAST of IVF0016565 vs. ExPASy Swiss-Prot
Match: O74184 (Target of rapamycin complex subunit wat1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pop3 PE=1 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.8e-107
Identity = 173/309 (55.99%), Postives = 233/309 (75.40%), Query Frame = 0


Query: 3   QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFD 62
           Q SV+L ++ YDHTIRFWEA SG C RTIQ+ DSQVNRL I+PDK++LAAAGNPH+RL+D
Sbjct: 8   QHSVLLVSSGYDHTIRFWEALSGICSRTIQHADSQVNRLCISPDKKFLAAAGNPHVRLYD 67

Query: 63  INSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAV 122
           IN++S  P+M+++ HTNNV ++ F CDG W+ + SEDGTVK+WD+RAP  QR Y+ ++ V
Sbjct: 68  INTSSQMPLMTFEGHTNNVTAIAFHCDGKWLATSSEDGTVKVWDMRAPSVQRNYDHKSPV 127

Query: 123 NTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANN 182
           N +++HPNQ EL+S DQ+G +R WDL  NSC+ EL+PE D  + S+TV  DGS+++A NN
Sbjct: 128 NDLLIHPNQGELLSCDQSGRVRAWDLGENSCTHELIPEEDVPMSSITVGSDGSMLIAGNN 187

Query: 183 HGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIW 242
            G CYVWR+L   Q  +  +P+ K QAH+ YI +C+LSP+     ++LAT S+D+TV IW
Sbjct: 188 KGNCYVWRML-NHQGASLLQPVVKFQAHQRYITRCVLSPDV----KHLATCSADATVNIW 247

Query: 243 NVD--GFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 302
           + +   F LE+ L GHQRWVWDC FS D  YL+TASSD  ARLW +S+G+ ++ Y GHHK
Sbjct: 248 STEDMSFMLERRLQGHQRWVWDCAFSADSTYLVTASSDHVARLWELSSGETIRQYSGHHK 307

Query: 303 ATICCALHD 310
           A +C AL+D
Sbjct: 308 AAVCVALND 311

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BLAST of IVF0016565 vs. ExPASy TrEMBL
Match: A0A5D3DPG8 (Target of rapamycin complex subunit LST8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005750 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 9.8e-191
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 317
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

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BLAST of IVF0016565 vs. ExPASy TrEMBL
Match: A0A0A0LH11 (Target of rapamycin complex subunit LST8 OS=Cucumis sativus OX=3659 GN=Csa_3G811590 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 9.8e-191
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 317
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

[Close]

BLAST of IVF0016565 vs. ExPASy TrEMBL
Match: A0A1S3B820 (Target of rapamycin complex subunit LST8 OS=Cucumis melo OX=3656 GN=LOC103486798 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 9.8e-191
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 317
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

[Close]

BLAST of IVF0016565 vs. ExPASy TrEMBL
Match: A0A6J1DLL8 (Target of rapamycin complex subunit LST8 OS=Momordica charantia OX=3673 GN=LOC111021575 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.2e-190
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVN+LEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNKLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 317
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

[Close]

BLAST of IVF0016565 vs. ExPASy TrEMBL
Match: A0A6J1FAK4 (Target of rapamycin complex subunit LST8 OS=Cucurbita moschata OX=3662 GN=LOC111442300 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.4e-189
Identity = 313/315 (99.37%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FD+NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDLNSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSS 316
           ATICCALHDGAEPSS
Sbjct: 301 ATICCALHDGAEPSS 315

[Close]

BLAST of IVF0016565 vs. NCBI nr
Match: XP_004136587.1 (target of rapamycin complex subunit LST8-1 [Cucumis sativus] >XP_008443103.1 PREDICTED: protein LST8 homolog [Cucumis melo] >XP_011652119.1 target of rapamycin complex subunit LST8-1 [Cucumis sativus] >KAA0043702.1 protein LST8-like protein [Cucumis melo var. makuwa] >TYK25432.1 protein LST8-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 671 bits (1730), Expect = 9.46e-244
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 316
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

[Close]

BLAST of IVF0016565 vs. NCBI nr
Match: XP_022154284.1 (protein LST8 homolog [Momordica charantia])

HSP 1 Score: 669 bits (1727), Expect = 2.71e-243
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVN+LEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNKLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSSG 316
           ATICCALHDGAEPSSG
Sbjct: 301 ATICCALHDGAEPSSG 316

[Close]

BLAST of IVF0016565 vs. NCBI nr
Match: XP_023528357.1 (protein LST8 homolog [Cucurbita pepo subsp. pepo] >XP_023528358.1 protein LST8 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669 bits (1725), Expect = 5.47e-243
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSS 315
           ATICCALHDGAEPSS
Sbjct: 301 ATICCALHDGAEPSS 315

[Close]

BLAST of IVF0016565 vs. NCBI nr
Match: XP_038906292.1 (target of rapamycin complex subunit LST8-1 [Benincasa hispida] >XP_038906293.1 target of rapamycin complex subunit LST8-1 [Benincasa hispida])

HSP 1 Score: 669 bits (1725), Expect = 5.47e-243
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSS 315
           ATICCALHDGAEPSS
Sbjct: 301 ATICCALHDGAEPSS 315

[Close]

BLAST of IVF0016565 vs. NCBI nr
Match: XP_022935415.1 (protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935416.1 protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935417.1 protein LST8 homolog isoform X1 [Cucurbita moschata] >KAG7017580.1 Protein LST8-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666 bits (1719), Expect = 4.50e-242
Identity = 313/315 (99.37%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FD+NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA
Sbjct: 61  FDLNSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK
Sbjct: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK
Sbjct: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300

Query: 301 ATICCALHDGAEPSS 315
           ATICCALHDGAEPSS
Sbjct: 301 ATICCALHDGAEPSS 315

[Close]

BLAST of IVF0016565 vs. TAIR 10
Match: AT3G18140.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 8.3e-166
Identity = 268/309 (86.73%), Postives = 285/309 (92.23%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDLRAPGCQ+EYES A
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDLRAPGCQKEYESVA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELVPEVDTAVRSLTVMWDG++VVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVPEVDTAVRSLTVMWDGTMVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVK 240
           NN GTCYVWRLLRG QTMT FEPLHKLQAH G+ILKCLLSP     ++YLATASSD TVK
Sbjct: 181 NNRGTCYVWRLLRGKQTMTEFEPLHKLQAHNGHILKCLLSP----ANKYLATASSDKTVK 240

Query: 241 IWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHK 300
           IWNVDGF LEK L GHQRWVWDCVFSVDG +L+TASSD TARLWSM  GKE+KVYQGHHK
Sbjct: 241 IWNVDGFKLEKVLTGHQRWVWDCVFSVDGEFLVTASSDMTARLWSMPAGKEVKVYQGHHK 300

Query: 301 ATICCALHD 310
           AT+CCALHD
Sbjct: 301 ATVCCALHD 305

[Close]

BLAST of IVF0016565 vs. TAIR 10
Match: AT2G22040.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.1e-128
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 6   VILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINS 65
           V LATAS+D TIR W+A++GRCY + +YPD  VNRLE+TP+K  L AA NPHIRLFD+ S
Sbjct: 11  VYLATASHDQTIRLWQARTGRCYFSFRYPDLHVNRLELTPEKGKLVAACNPHIRLFDLRS 70

Query: 66  NSPQ-PIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNT 125
            +P  P+ ++ SHT NVM+VGFQ  G+ MYSGSEDG+VKIWDLR   CQRE+ S + VNT
Sbjct: 71  YNPHIPVRNFVSHTKNVMAVGFQYTGHMMYSGSEDGSVKIWDLRVRECQREFRSVSPVNT 130

Query: 126 VVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNHG 185
           VVLHPNQTELISGDQNGNIRVWDL A+ CSCELVPEV T +RSLTVMWDG++VVAAN+ G
Sbjct: 131 VVLHPNQTELISGDQNGNIRVWDLRADLCSCELVPEVGTPIRSLTVMWDGTMVVAANDRG 190

Query: 186 TCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIWNV 245
           TCYVWR L   QTMT FEPLHKLQAH  +ILKCLLSP     +RYLATASSD TVKIWN+
Sbjct: 191 TCYVWRSLCERQTMTEFEPLHKLQAHNSHILKCLLSP---GNNRYLATASSDKTVKIWNL 250

Query: 246 DGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATIC 305
           DGF LEK L GH+RWVWDC FS+DG YL+TASSDTTARLWSM  GKE  VYQ H KAT+C
Sbjct: 251 DGFKLEKVLTGHERWVWDCDFSMDGEYLVTASSDTTARLWSMRAGKEEMVYQAHRKATVC 310

Query: 306 CAL 308
           C L
Sbjct: 311 CTL 310

[Close]

BLAST of IVF0016565 vs. TAIR 10
Match: AT3G18140.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.8e-121
Identity = 199/221 (90.05%), Postives = 210/221 (95.02%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRA 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDLRAPGCQ+EYES A
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDLRAPGCQKEYESVA 120

Query: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAA 180
           AVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELVPEVDTAVRSLTVMWDG++VVAA
Sbjct: 121 AVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVPEVDTAVRSLTVMWDGTMVVAA 180

Query: 181 NNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSP 222
           NN GTCYVWRLLRG QTMT FEPLHKLQAH G+ILKCLLSP
Sbjct: 181 NNRGTCYVWRLLRGKQTMTEFEPLHKLQAHNGHILKCLLSP 221

[Close]

BLAST of IVF0016565 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-25
Identity = 72/305 (23.61%), Postives = 142/305 (46.56%), Query Frame = 0


Query: 29  RTIQYPDSQVNRLEITPDKRYLA-AAGNPHIRLFDINS-NSP--QPIMSYDSHTNNVMSV 88
           +T+   +  V+ ++ + D R LA A+ +  IR + IN+ N P  +P+  +  H N +  V
Sbjct: 18  QTLTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPIAEPVQEFTGHENGISDV 77

Query: 89  GFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNTVVLH----------PNQTEL 148
            F  D  ++ S S+D T+K+WD+         E+ + + T++ H          P    +
Sbjct: 78  AFSSDARFIVSASDDKTLKLWDV---------ETGSLIKTLIGHTNYAFCVNFNPQSNMI 137

Query: 149 ISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNHGTCYVWRLLRG 208
           +SG  +  +R+WD+T   C  +++P     V ++    DGSL+V+++  G C +W     
Sbjct: 138 VSGSFDETVRIWDVTTGKC-LKVLPAHSDPVTAVDFNRDGSLIVSSSYDGLCRIW----- 197

Query: 209 TQTMTNFEPLHKLQAHKGYILKCLLSPE--------FCEPHRYLATASSDSTVKIWNVDG 268
                         +  G+ +K L+  E        F    +++   + D+T+++WN+  
Sbjct: 198 -------------DSGTGHCVKTLIDDENPPVSFVRFSPNGKFILVGTLDNTLRLWNISS 257

Query: 269 FTLEKTLIGH--QRWVWDCVFSV-DGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATI 309
               KT  GH   ++     FSV +G  +++ S D    +W +++ K ++  +GH +  +
Sbjct: 258 AKFLKTYTGHVNAQYCISSAFSVTNGKRIVSGSEDNCVHMWELNSKKLLQKLEGHTETVM 294

[Close]

BLAST of IVF0016565 vs. TAIR 10
Match: AT4G02730.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 7.3e-21
Identity = 69/292 (23.63%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 23  KSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINSNSPQPIMSYDSHTNNVM 82
           K  R  +T++   + ++ ++ + D   LA+A      +   ++ +   I  Y+ H++ + 
Sbjct: 31  KPYRHLKTLEGHTAAISCVKFSNDGNLLASASVDKTMIL-WSATNYSLIHRYEGHSSGIS 90

Query: 83  SVGFQCDGNWMYSGSEDGTVKIWDLRAP-GCQREYESRA-AVNTVVLHPNQTELISGDQN 142
            + +  D ++  S S+D T++IWD R+P  C +        V  V  +P    ++SG  +
Sbjct: 91  DLAWSSDSHYTCSASDDCTLRIWDARSPYECLKVLRGHTNFVFCVNFNPPSNLIVSGSFD 150

Query: 143 GNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTN 202
             IR+W++    C   ++      + S+    DGSL+V+A++ G+C +W    GT   T 
Sbjct: 151 ETIRIWEVKTGKC-VRMIKAHSMPISSVHFNRDGSLIVSASHDGSCKIWDAKEGTCLKTL 210

Query: 203 FEPLHKLQAHKGYILKCLLSPEFCEPHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWV 262
            +               +   +F    +++  A+ DST+K+ N       K   GH   V
Sbjct: 211 IDDKS----------PAVSFAKFSPNGKFILVATLDSTLKLSNYATGKFLKVYTGHTNKV 270

Query: 263 WDCV---FSV-DGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATICCALH 309
           + C+   FSV +G Y+++ S D    LW +     ++  +GH  A I  + H
Sbjct: 271 F-CITSAFSVTNGKYIVSGSEDNCVYLWDLQARNILQRLEGHTDAVISVSCH 309

The following BLAST results are available for this feature:

BLAST of IVF0016565 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AYE4	3.3e-167	85.90	Target of rapamycin complex subunit LST8 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9LV27	1.2e-164	86.73	Target of rapamycin complex subunit LST8-1 OS=Arabidopsis thaliana OX=3702 GN=LS...	[more]
F4IIK6	1.5e-127	71.29	Non-functional target of rapamycin complex subunit LST8-2 OS=Arabidopsis thalian...	[more]
Q54D08	4.5e-108	60.13	Protein LST8 homolog OS=Dictyostelium discoideum OX=44689 GN=lst8 PE=1 SV=1	[more]
O74184	3.8e-107	55.99	Target of rapamycin complex subunit wat1 OS=Schizosaccharomyces pombe (strain 97...	[more]

BLAST of IVF0016565 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DPG8	9.8e-191	100.00	Target of rapamycin complex subunit LST8 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0LH11	9.8e-191	100.00	Target of rapamycin complex subunit LST8 OS=Cucumis sativus OX=3659 GN=Csa_3G811...	[more]
A0A1S3B820	9.8e-191	100.00	Target of rapamycin complex subunit LST8 OS=Cucumis melo OX=3656 GN=LOC103486798...	[more]
A0A6J1DLL8	2.2e-190	99.68	Target of rapamycin complex subunit LST8 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FAK4	2.4e-189	99.37	Target of rapamycin complex subunit LST8 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of IVF0016565 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136587.1	9.46e-244	100.00	target of rapamycin complex subunit LST8-1 [Cucumis sativus] >XP_008443103.1 PRE...	[more]
XP_022154284.1	2.71e-243	99.68	protein LST8 homolog [Momordica charantia]	[more]
XP_023528357.1	5.47e-243	100.00	protein LST8 homolog [Cucurbita pepo subsp. pepo] >XP_023528358.1 protein LST8 h...	[more]
XP_038906292.1	5.47e-243	100.00	target of rapamycin complex subunit LST8-1 [Benincasa hispida] >XP_038906293.1 t...	[more]
XP_022935415.1	4.50e-242	99.37	protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935416.1 protein LST...	[more]

BLAST of IVF0016565 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18140.1	8.3e-166	86.73	Transducin/WD40 repeat-like superfamily protein	[more]
AT2G22040.1	1.1e-128	71.29	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G18140.2	4.8e-121	90.05	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G49660.1	1.7e-25	23.61	Transducin/WD40 repeat-like superfamily protein	[more]
AT4G02730.1	7.3e-21	23.63	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020472	G-protein beta WD-40 repeat	PRINTS	PR00320	GPROTEINBRPT	coord: 230..244 score: 39.97 coord: 93..107 score: 41.78 coord: 272..286 score: 31.09
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 246..285 e-value: 1.4E-7 score: 41.2 coord: 109..147 e-value: 3.3E-4 score: 30.0 coord: 67..106 e-value: 1.1E-9 score: 48.2 coord: 150..190 e-value: 13.0 score: 10.0 coord: 200..243 e-value: 1.0E-5 score: 35.0 coord: 24..62 e-value: 0.76 score: 17.7
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 36..62 e-value: 0.19 score: 12.7 coord: 202..243 e-value: 2.7E-4 score: 21.6 coord: 69..106 e-value: 1.6E-8 score: 35.0 coord: 112..147 e-value: 0.011 score: 16.6 coord: 250..285 e-value: 7.6E-6 score: 26.6
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 115..156 score: 10.608706
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 1..30 score: 10.074014
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 253..294 score: 13.516088
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 207..252 score: 10.575287
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 74..108 score: 15.220417
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 5..310 e-value: 2.0E-101 score: 340.9
IPR037588	Target of rapamycin complex subunit LST8	PANTHER	PTHR19842	G BETA-LIKE PROTEIN GBL	coord: 3..310
None	No IPR available	PANTHER	PTHR19842:SF0	TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8	coord: 3..310
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 74..108 score: 12.551949
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 115..148 score: 8.834696
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 253..290 score: 10.179234
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 207..245 score: 8.808332
None	No IPR available	CDD	cd00200	WD40	coord: 7..285 e-value: 1.626E-52 score: 172.519
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 93..107
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 8..22
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 134..148
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 230..244
IPR011047	Quinoprotein alcohol dehydrogenase-like superfamily	SUPERFAMILY	50998	Quinoprotein alcohol dehydrogenase-like	coord: 7..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0016565.2	IVF0016565.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032956	regulation of actin cytoskeleton organization
biological_process	GO:0031929	TOR signaling
cellular_component	GO:0031931	TORC1 complex
cellular_component	GO:0031932	TORC2 complex
molecular_function	GO:0005515	protein binding