IVF0013545 (gene) Melon (IVF77) v1

Overview
NameIVF0013545
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6
Locationchr06: 20156925 .. 20159213 (+)
RNA-Seq ExpressionIVF0013545
SyntenyIVF0013545
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAACCCTCATCCTTTCTGTCTCCTCTCTTTCTCCTAACAAACTCCATCTCTCATTCCTCATTCATTCGTTCATTTGAAAAATGCCTGAAGCACCAAAACCAAGTACTCCAAATTACAACCAGCTCGTTCAAAAAAACAAAGCCATTCTTCAATTCATTGAAGATGTCACCACCAACGCTGCTCTTGTCCAACGCCAAGTCCTCTCTCAAATCCTCTCCCAAAATTCCAACTCCGAGTACTTAACGCTCTATGGTCGCCCCTCTCCTGATACCTTCAAAACCTCCATCCCTCTCGTCTCTTACGACCAAATCCAACCCTTCGTCTCTCGTATTGCCAATGGCGATTTCTCCCCTATTCTATGCTCCTCTCCCATCTCTGAATTCTTAACCAGGTCTAACCTCGGACGTGACATGATGACACTAATTTCTTTTTTGTTTCATTTCTTTGGTTTGTGGATTATTAATTATGATCTCATGTTTTGGATATTTGGTTTTGCAGCTCTGGCACCTCGGGTGGCGAGAGAAAGTTGATGCCGACGATCGAGGAGGAGTTGGAGAGACGATCATTGTTGTATAGCTTGTTGATGCCTGTGATGTCTCAATTCGTTCCTGGCTTAGAGAAGGGTAAAGGAATGTATTTTTTGTTTATTAAAGCTGAGGCCAAAACCCCTGGTGGCTTGCTCGCTCGCCCCGTTTTAACTAGCTACTACAAGAGCTCTCATTTTAAGGAACGCCCTTACGATCCTTATACCAATTACACCAGCCCCAATGAAGCTATTCTTTGCCCCGACTCCTATCAAAGCATGTACGCTCAATTGCTCTGTGGTCTTTGTCATCGTCTCGACGTTCTTCGCGTCGGTGCTGTTTTTGCCTCTGGTTTCATTCGTGCTATTCGCTTTCTTGAAAAGCATTGGCAACTTCTCTGCCACGATATCCGCACTGGGACGCTGAATTCCCAGATCACAGACCAGGCGGTTCGTGATGCAGTTATGTTATCGGTGCTACGTAGGCCGGATCCTGAGTTGGCAGATTATATTCATGGAGAGTGTTGTAAGGGATCTTGGCAGGGAATTATTACGAGGTTGTGGCCGAATACGAAATACGTGGATGTGATTGTTACCGGGACGATGTCACAGTATATTGCAACGCTTGATTATTATAGCAACGGATTGCCTTTGGTTTGTACTATGTATGCTTCTTCAGAGTGTTATTTTGGTGTGAATCTTAACCCTCTTTGTAAACCAAGTGAAGTTGCTTATACTCTCATTCCTTCCATGGCATATTTTGAGTTTCTTCCTATTGAAAGAAGCCACAACAACGATGACAACTCCCTCGATGAGCAACAACTTGTCGACCTAACCGATGTTGAACTCGGTAAAGAATATGAGCTCGTCGTCACGACTTACGCCGGTAATTAATTATTATGCTTCTCTCTTTTTTTCTCTTTTTCTGTTGTGGGGTTTCTGAATAAGTTATGTTTGGAATAGGACTTTATCGATATCGAGTGGGCGATATCCTAAGAGTAGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATATGTCGAAAGAACGTGGTTCTAAGCATCGATTCCGATAAAACCGATGAAGTAGAGCTTCAAAACGCAGTGAAAAACTCAATGAACCATTTAGTGCCATTCGAGGCGACATTGGCGGAGTACACAAGCTATGCCAACACTTCAACAATTCCAGGTCACTACGTGTTATATTGGGAGGTAAACCAAAAGGAAGGGGCAGCCACACCGGTGCCGCCGTCCGTGTTGGAAGATTGTTGTCTTACGATTGAGGAGTCATTGAACAGCGTATACCGGCAGGGGAGAGTATCGGACAAGTCAATCGGGCCACTGGAAATCAAGGTTGTGGAAAATGGGACATTTGATAAGCTGATGGATTATGCTATAAGCATGGGAGCTTCAATAAATCAGTACAAAACCCCAAGGTGTGTGAAGTTTCAACCCATTGTTGAGCTTTTGAACTCAAGAGTTGTGGGTTCTTATTTCAGCCCTAAGTGCCCAAAGTGGGTTCCTGGTCATAAGCAATGGTCCCAACAAGATTAGTTCCTTAATTTTCATAATATTCTTCAATGGTCTATTGTACCCTCTTGGTTTTCCCTCTCAAATAATTTGTATGGCTCTTCTCATCATGATCATCTTAATTAGTACTAATGTACTAGGCAATTCTCATCTTTTTTCATAGCTTCTAATTTTTCTTGTTTGGATATATTAGACCCTTTTCTATCCCATATGTAAAAATTGAATTCGAAAAA

mRNA sequence

AGAACCCTCATCCTTTCTGTCTCCTCTCTTTCTCCTAACAAACTCCATCTCTCATTCCTCATTCATTCGTTCATTTGAAAAATGCCTGAAGCACCAAAACCAAGTACTCCAAATTACAACCAGCTCGTTCAAAAAAACAAAGCCATTCTTCAATTCATTGAAGATGTCACCACCAACGCTGCTCTTGTCCAACGCCAAGTCCTCTCTCAAATCCTCTCCCAAAATTCCAACTCCGAGTACTTAACGCTCTATGGTCGCCCCTCTCCTGATACCTTCAAAACCTCCATCCCTCTCGTCTCTTACGACCAAATCCAACCCTTCGTCTCTCGTATTGCCAATGGCGATTTCTCCCCTATTCTATGCTCCTCTCCCATCTCTGAATTCTTAACCAGCTCTGGCACCTCGGGTGGCGAGAGAAAGTTGATGCCGACGATCGAGGAGGAGTTGGAGAGACGATCATTGTTGTATAGCTTGTTGATGCCTGTGATGTCTCAATTCGTTCCTGGCTTAGAGAAGGGTAAAGGAATGTATTTTTTGTTTATTAAAGCTGAGGCCAAAACCCCTGGTGGCTTGCTCGCTCGCCCCGTTTTAACTAGCTACTACAAGAGCTCTCATTTTAAGGAACGCCCTTACGATCCTTATACCAATTACACCAGCCCCAATGAAGCTATTCTTTGCCCCGACTCCTATCAAAGCATGTACGCTCAATTGCTCTGTGGTCTTTGTCATCGTCTCGACGTTCTTCGCGTCGGTGCTGTTTTTGCCTCTGGTTTCATTCGTGCTATTCGCTTTCTTGAAAAGCATTGGCAACTTCTCTGCCACGATATCCGCACTGGGACGCTGAATTCCCAGATCACAGACCAGGCGGTTCGTGATGCAGTTATGTTATCGGTGCTACGTAGGCCGGATCCTGAGTTGGCAGATTATATTCATGGAGAGTGTTGTAAGGGATCTTGGCAGGGAATTATTACGAGGTTGTGGCCGAATACGAAATACGTGGATGTGATTGTTACCGGGACGATGTCACAGTATATTGCAACGCTTGATTATTATAGCAACGGATTGCCTTTGGTTTGTACTATGTATGCTTCTTCAGAGTGTTATTTTGGTGTGAATCTTAACCCTCTTTGTAAACCAAGTGAAGTTGCTTATACTCTCATTCCTTCCATGGCATATTTTGAGTTTCTTCCTATTGAAAGAAGCCACAACAACGATGACAACTCCCTCGATGAGCAACAACTTGTCGACCTAACCGATGTTGAACTCGGTAAAGAATATGAGCTCGTCGTCACGACTTACGCCGGACTTTATCGATATCGAGTGGGCGATATCCTAAGAGTAGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATATGTCGAAAGAACGTGGTTCTAAGCATCGATTCCGATAAAACCGATGAAGTAGAGCTTCAAAACGCAGTGAAAAACTCAATGAACCATTTAGTGCCATTCGAGGCGACATTGGCGGAGTACACAAGCTATGCCAACACTTCAACAATTCCAGGTCACTACGTGTTATATTGGGAGGTAAACCAAAAGGAAGGGGCAGCCACACCGGTGCCGCCGTCCGTGTTGGAAGATTGTTGTCTTACGATTGAGGAGTCATTGAACAGCGTATACCGGCAGGGGAGAGTATCGGACAAGTCAATCGGGCCACTGGAAATCAAGGTTGTGGAAAATGGGACATTTGATAAGCTGATGGATTATGCTATAAGCATGGGAGCTTCAATAAATCAGTACAAAACCCCAAGGTGTGTGAAGTTTCAACCCATTGTTGAGCTTTTGAACTCAAGAGTTGTGGGTTCTTATTTCAGCCCTAAGTGCCCAAAGTGGGTTCCTGGTCATAAGCAATGGTCCCAACAAGATTAGTTCCTTAATTTTCATAATATTCTTCAATGGTCTATTGTACCCTCTTGGTTTTCCCTCTCAAATAATTTGTATGGCTCTTCTCATCATGATCATCTTAATTAGTACTAATGTACTAGGCAATTCTCATCTTTTTTCATAGCTTCTAATTTTTCTTGTTTGGATATATTAGACCCTTTTCTATCCCATATGTAAAAATTGAATTCGAAAAA

Coding sequence (CDS)

ATGCCTGAAGCACCAAAACCAAGTACTCCAAATTACAACCAGCTCGTTCAAAAAAACAAAGCCATTCTTCAATTCATTGAAGATGTCACCACCAACGCTGCTCTTGTCCAACGCCAAGTCCTCTCTCAAATCCTCTCCCAAAATTCCAACTCCGAGTACTTAACGCTCTATGGTCGCCCCTCTCCTGATACCTTCAAAACCTCCATCCCTCTCGTCTCTTACGACCAAATCCAACCCTTCGTCTCTCGTATTGCCAATGGCGATTTCTCCCCTATTCTATGCTCCTCTCCCATCTCTGAATTCTTAACCAGCTCTGGCACCTCGGGTGGCGAGAGAAAGTTGATGCCGACGATCGAGGAGGAGTTGGAGAGACGATCATTGTTGTATAGCTTGTTGATGCCTGTGATGTCTCAATTCGTTCCTGGCTTAGAGAAGGGTAAAGGAATGTATTTTTTGTTTATTAAAGCTGAGGCCAAAACCCCTGGTGGCTTGCTCGCTCGCCCCGTTTTAACTAGCTACTACAAGAGCTCTCATTTTAAGGAACGCCCTTACGATCCTTATACCAATTACACCAGCCCCAATGAAGCTATTCTTTGCCCCGACTCCTATCAAAGCATGTACGCTCAATTGCTCTGTGGTCTTTGTCATCGTCTCGACGTTCTTCGCGTCGGTGCTGTTTTTGCCTCTGGTTTCATTCGTGCTATTCGCTTTCTTGAAAAGCATTGGCAACTTCTCTGCCACGATATCCGCACTGGGACGCTGAATTCCCAGATCACAGACCAGGCGGTTCGTGATGCAGTTATGTTATCGGTGCTACGTAGGCCGGATCCTGAGTTGGCAGATTATATTCATGGAGAGTGTTGTAAGGGATCTTGGCAGGGAATTATTACGAGGTTGTGGCCGAATACGAAATACGTGGATGTGATTGTTACCGGGACGATGTCACAGTATATTGCAACGCTTGATTATTATAGCAACGGATTGCCTTTGGTTTGTACTATGTATGCTTCTTCAGAGTGTTATTTTGGTGTGAATCTTAACCCTCTTTGTAAACCAAGTGAAGTTGCTTATACTCTCATTCCTTCCATGGCATATTTTGAGTTTCTTCCTATTGAAAGAAGCCACAACAACGATGACAACTCCCTCGATGAGCAACAACTTGTCGACCTAACCGATGTTGAACTCGGTAAAGAATATGAGCTCGTCGTCACGACTTACGCCGGACTTTATCGATATCGAGTGGGCGATATCCTAAGAGTAGCGGGATTCAAAAACAAAGCTCCACAATTCAACTTCATATGTCGAAAGAACGTGGTTCTAAGCATCGATTCCGATAAAACCGATGAAGTAGAGCTTCAAAACGCAGTGAAAAACTCAATGAACCATTTAGTGCCATTCGAGGCGACATTGGCGGAGTACACAAGCTATGCCAACACTTCAACAATTCCAGGTCACTACGTGTTATATTGGGAGGTAAACCAAAAGGAAGGGGCAGCCACACCGGTGCCGCCGTCCGTGTTGGAAGATTGTTGTCTTACGATTGAGGAGTCATTGAACAGCGTATACCGGCAGGGGAGAGTATCGGACAAGTCAATCGGGCCACTGGAAATCAAGGTTGTGGAAAATGGGACATTTGATAAGCTGATGGATTATGCTATAAGCATGGGAGCTTCAATAAATCAGTACAAAACCCCAAGGTGTGTGAAGTTTCAACCCATTGTTGAGCTTTTGAACTCAAGAGTTGTGGGTTCTTATTTCAGCCCTAAGTGCCCAAAGTGGGTTCCTGGTCATAAGCAATGGTCCCAACAAGATTAG

Protein sequence

MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD
Homology
BLAST of IVF0013545 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 5.3e-275
Identity = 468/613 (76.35%), Postives = 529/613 (86.30%), Query Frame = 0

Query: 1   MPEAPKPSTPNYN--QLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLT--- 60
           MPEAPK +    +   L +KNK  LQFIEDVTTNA  VQR+VL +ILS+N++ EYL    
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  LYGRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLM 120
           L GR   +TFK  +P+V+Y+ IQP ++RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVM QFVPGL+KGKGMYFLFIK+E+KTPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK RPYDPYTNYTSPN+ ILC DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGI 300
           +FLEKHW  L  DIRTGTL+S+ITD +VR+AV    + +PDP+LAD++  EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPIERSHNNDDN--------SLDEQQLVDLTDVELGKEYELVVTTYA 420
           +YTLIP+MAYFEFLP+ R+     +          ++Q+LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFE 480
           GLYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN++ HLVPF+
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLAEYTSYANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRV 540
           A+L+EYTSYA+TS+IPGHYVL+WE+       TP+PPSV EDCCLTIEESLNSVYRQGRV
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCL--NGNTPIPPSVFEDCCLTIEESLNSVYRQGRV 540

Query: 541 SDKSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFS 600
           SDKSIGPLEIK+VE+GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV SYFS
Sbjct: 541 SDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFS 600

BLAST of IVF0013545 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 2.5e-272
Identity = 467/614 (76.06%), Postives = 527/614 (85.83%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLV--QKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYL---T 60
           MPEAPK  +     L   QKNK  LQ IE++T+NA  VQRQVL +IL++N++ EYL    
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  LYGRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLM 120
           L GR   +TFK  +P+++Y+ I+P ++RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVMSQFVPGLE GKGMYFLFIK+E+KTPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFKERPYDPYTNYTSPNE ILC DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGI 300
           +FLEKHW  L  DIRTGTL+S ITD +VR+AV  + + +P P+LAD++  EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPIERSHNNDDNSL---------DEQQLVDLTDVELGKEYELVVTTY 420
           +YTLIPSMAYFEFLP+ R +N   NS+         ++Q+LVDL DV+LG+EYELVVTTY
Sbjct: 361 SYTLIPSMAYFEFLPVHR-NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 420

Query: 421 AGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPF 480
           AGL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN++ HLVPF
Sbjct: 421 AGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 480

Query: 481 EATLAEYTSYANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGR 540
           +A+L+EYTSYA+TS+IPGHYVL+WE+       TP+PPSV EDCCL +EES N+VYRQGR
Sbjct: 481 DASLSEYTSYADTSSIPGHYVLFWELCL--DGNTPIPPSVFEDCCLAVEESFNTVYRQGR 540

Query: 541 VSDKSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYF 600
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV SYF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

BLAST of IVF0013545 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 5.4e-243
Identity = 415/607 (68.37%), Postives = 484/607 (79.74%), Query Frame = 0

Query: 1   MPEAPKPST-PNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGR 60
           MPEAP   T P Y      +   L+FIE VT NA  VQR+VL +IL+QN+ +EYL  YG 
Sbjct: 1   MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGI 60

Query: 61  P-SP---DTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLM 120
           P SP   D F+  +PLV+Y+ +QP + RIANGD SPI    PISEFLTSSGTSGGERKLM
Sbjct: 61  PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYK 180
           PTI +E+ RRSLLYSLLMPVMSQ V GL+KGK MY LF+KAE++TPGGL ARPVLTSYY+
Sbjct: 121 PTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYR 180

Query: 181 SSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAI 240
           S  F +RP DPYT+YTSP+EAILC DSYQSMYAQLLCGL HR DVLRVGAVFASGF+RAI
Sbjct: 181 SRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAI 240

Query: 241 RFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGI 300
            FLEKHW  LCHDIRTG L+ +ITD+ VRDAV    + R DP LAD I  EC + SW+GI
Sbjct: 241 HFLEKHWARLCHDIRTGELDPEITDRVVRDAV--GRVLRADPALADAIEDECARASWEGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEV 360
           I RLWP TKY+DVIVTGTMSQYI TL++Y  GLPL CTMYASSECYFG+NLNP+CKPS+V
Sbjct: 301 IRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDV 360

Query: 361 AYTLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVG 420
           AYTLIP+M Y+EFLP+  + NN       + LVDL DV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 AYTLIPTMCYYEFLPV--NCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVG 420

Query: 421 DILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTS 480
           D+LRVAGFKNKAP F+F+ R+NV LS+DSDKTDE EL  AV  ++ HL PF A+L EYTS
Sbjct: 421 DVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTS 480

Query: 481 YANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPL 540
           YA+ +TIPGHYVL+WE+      +T VP SV E+CCL++EE+LNSVYRQGR  D+SIGPL
Sbjct: 481 YADAATIPGHYVLFWEL---RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPL 540

Query: 541 EIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVP 600
           EI+VV  GTFDKLMDYAIS GASINQYK PRCV+  P+VELL++RV G YFSPKCPKW P
Sbjct: 541 EIRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 600

Query: 601 GHKQWSQ 603
           G+KQW++
Sbjct: 601 GNKQWNK 600

BLAST of IVF0013545 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.8e-231
Identity = 405/612 (66.18%), Postives = 474/612 (77.45%), Query Frame = 0

Query: 1   MPE----APKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTL 60
           MPE    A  P+      +  +++  L+ IE VT NA   QR+VL +IL+QN+ +EYL  
Sbjct: 1   MPEAVAAAVSPAAAAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRR 60

Query: 61  YGRP-----SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGE 120
            G P     + + F+   PLV+Y+ I P V RIANGD SPIL   P+SEFLTSSGTSGGE
Sbjct: 61  LGVPGDAPGADEAFRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLT 180
           RKLMPTIEEE+ERRS LYSLLMPVMS+ VPGL+KGK MY  F+K+E +TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEEMERRSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLT 180

Query: 181 SYYKSSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGF 240
           S+Y+S +F ERP+DPYT YTSP+EA+LC D+YQSMYAQL+CGL HR DVLRVGAVFASGF
Sbjct: 181 SFYRSRYFLERPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGS 300
           +RAIRFLEKHW  LC DIR G L+  +TD AVR AV   VLR  DP LAD I  EC + S
Sbjct: 241 LRAIRFLEKHWPSLCRDIRAGELDGGVTDPAVRGAVG-RVLRGADPALADAIEAECARPS 300

Query: 301 WQGIITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCK 360
           WQGII R+WP+TKY+DVIVTG M+QYI TL++Y  GLPL CTMYASSECYFG+NLNP+CK
Sbjct: 301 WQGIIRRVWPSTKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCK 360

Query: 361 PSEVAYTLIPSMAYFEFLPIERSHNN-DDNSLDEQQLVDLTDVELGKEYELVVTTYAGLY 420
           PSEVAYTLIP+M YFEFLP+    N+      D + LVDL DV+LG EYELVVTTY+GLY
Sbjct: 361 PSEVAYTLIPTMCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLY 420

Query: 421 RYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATL 480
           RYRVGD+LRVAGFKN AP F F+ RKNV LSIDSDKTDE EL  AV  ++ HL PF A+L
Sbjct: 421 RYRVGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASL 480

Query: 481 AEYTSYANT-STIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSD 540
            EYTSYA+T +TIPGHYVL+WE+    G  TPVP SV EDCCL +EE LNSVYRQ R +D
Sbjct: 481 VEYTSYADTATTIPGHYVLFWELRSPAG-GTPVPASVFEDCCLAVEEGLNSVYRQCRAAD 540

Query: 541 KSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPK 600
           +SIGPLEI+VV +GTFDKLMDYA+S GASINQYK PRCV+  P+VELL+ RV  +YFSPK
Sbjct: 541 RSIGPLEIRVVADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPK 600

BLAST of IVF0013545 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.2e-223
Identity = 384/596 (64.43%), Postives = 467/596 (78.36%), Query Frame = 0

Query: 8   STPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYL---TLYGRPSPDT 67
           S+P      +K+   L+FIE++T NA  VQ  +L++IL++N+++EYL    L G    DT
Sbjct: 8   SSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDT 67

Query: 68  FKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELER 127
           FKT IP+++Y+ +QP + RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+R
Sbjct: 68  FKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDR 127

Query: 128 RSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPY 187
           R LLYSLLMPVM+ +VPGL+KGKGMYFLF+K+E KTPGGL ARPVLTSYYKS HF+ RPY
Sbjct: 128 RQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPY 187

Query: 188 DPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 247
           DPY  YTSPNEAILCPDS+QSMY Q+LCGL  RL VLRVGAVFASG +RAIRFL+ HW  
Sbjct: 188 DPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSR 247

Query: 248 LCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTK 307
             HDI  G L+S+ITD ++R    +S + +PDP LA++I  EC   +W+ IITR+WPNTK
Sbjct: 248 FAHDIELGCLDSEITDPSIRQC--MSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTK 307

Query: 308 YVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMA 367
           Y+DVIVTG M+QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEV+YT++P+MA
Sbjct: 308 YLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMA 367

Query: 368 YFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFK 427
           YFEF+P           L   + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF 
Sbjct: 368 YFEFIP-----------LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 427

Query: 428 NKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHL-VPFEATLAEYTSYANTSTIP 487
           N APQF+F+ RKNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TSTIP
Sbjct: 428 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIP 487

Query: 488 GHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENG 547
           GHYVLYWE+  ++GA  P     L  CCL +EESLNSVYRQ RV+D S+GPLEI+VV NG
Sbjct: 488 GHYVLYWELLVRDGARQP-SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNG 547

Query: 548 TFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600
           TF++LMDYAIS GASINQYK PRCV F PIVELL+SRVV ++FSP  P W P  ++
Sbjct: 548 TFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of IVF0013545 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK
Sbjct: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW
Sbjct: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 605
           SQQD
Sbjct: 601 SQQD 604

BLAST of IVF0013545 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK
Sbjct: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW
Sbjct: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 605
           SQQD
Sbjct: 601 SQQD 604

BLAST of IVF0013545 vs. ExPASy TrEMBL
Match: A0A0A0L7R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G088930 PE=3 SV=1)

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 587/604 (97.19%), Postives = 597/604 (98.84%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPKPST NYNQL+QKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP
Sbjct: 1   MPEAPKPSTQNYNQLLQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           S DTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SSDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           EL+RRSLLYSLLMPVM+QFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELDRRSLLYSLLMPVMTQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK
Sbjct: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQAVRDAVM SVLR PDPELADY+HGECCKG WQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMSSVLRGPDPELADYVHGECCKGWWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYITTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLP+ERSH NDDNSL+EQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPVERSHINDDNSLNEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+N+L+PFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNNLMPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWE+NQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWELNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW
Sbjct: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 605
           SQQD
Sbjct: 601 SQQD 604

BLAST of IVF0013545 vs. ExPASy TrEMBL
Match: A0A6J1JAP2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483268 PE=3 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 557/607 (91.76%), Postives = 586/607 (96.54%), Query Frame = 0

Query: 1   MPEAPKPST---PNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLY 60
           MPEAPK +T   PNYN LVQKN AILQFIEDVTTNA LVQRQVLSQILS+N+ SEYL+L+
Sbjct: 1   MPEAPKTATSVSPNYN-LVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLH 60

Query: 61  GRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPT 120
           GRPSPDTFK+SIPLVSYD IQPF+SRIANGD SPILCSSPISEFLTSSGTSGGERKLMPT
Sbjct: 61  GRPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSS 180
           IEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIIT 300
           LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML VLRRPDPELAD+IH +CCKG W+GIIT
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML-VLRRPDPELADFIHEQCCKGFWKGIIT 300

Query: 301 RLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360
           RLWPNTKY+DVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY
Sbjct: 301 RLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360

Query: 361 TLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           TLIPSMAYFEFLP++R+ NND+ SLD+QQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI
Sbjct: 361 TLIPSMAYFEFLPVQRTSNNDE-SLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420

Query: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYA 480
           LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK S+NHLVPFEATLAEYTS+A
Sbjct: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAEYTSHA 480

Query: 481 NTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEI 540
           NTSTIPGHYVLYWE+NQK G+ATPVPPSVLEDCCLTIEESLNSVYRQGRV+DKSIGPLEI
Sbjct: 481 NTSTIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEI 540

Query: 541 KVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGH 600
           KVVE+GTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVG YFSPKCPKWV GH
Sbjct: 541 KVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGH 600

Query: 601 KQWSQQD 605
           KQWSQQD
Sbjct: 601 KQWSQQD 604

BLAST of IVF0013545 vs. ExPASy TrEMBL
Match: A0A6J1EW65 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111438458 PE=3 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 551/607 (90.77%), Postives = 580/607 (95.55%), Query Frame = 0

Query: 1   MPEAPKPS---TPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLY 60
           MPEAPK     TPNYN LVQKN AILQFIEDVTTNA LVQRQVLSQILS+N+ SEYL+L+
Sbjct: 1   MPEAPKTPTSVTPNYN-LVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLH 60

Query: 61  GRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPT 120
            RPSPDTFK+SIPLVSYD IQPF+SRIANGD SPILCSSPISEFLTSSGTSGGERKLMPT
Sbjct: 61  ARPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSS 180
           IEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIIT 300
           LEKHWQLLCHDIRTGTLNSQITDQAV DAVML VLRRPDPELAD+IH +C KG W+GIIT
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVGDAVML-VLRRPDPELADFIHEQCSKGYWKGIIT 300

Query: 301 RLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360
           RLWPNTKY+DVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY
Sbjct: 301 RLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360

Query: 361 TLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           TLIPSMAYFEFLP++R+ NN + SL++QQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI
Sbjct: 361 TLIPSMAYFEFLPVQRTSNNGE-SLNDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420

Query: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYA 480
           LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFEATLAEYTS+A
Sbjct: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSHA 480

Query: 481 NTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEI 540
           NTSTIPGHYVLYWE+NQK  + TPVPPSVLEDCCLTIEESLNSVYRQGRV+DKSIGPLEI
Sbjct: 481 NTSTIPGHYVLYWELNQKGSSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEI 540

Query: 541 KVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGH 600
           KVVE+GTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVG YFSPKCPKWV GH
Sbjct: 541 KVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGH 600

Query: 601 KQWSQQD 605
           KQWSQQD
Sbjct: 601 KQWSQQD 604

BLAST of IVF0013545 vs. NCBI nr
Match: XP_008452341.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA0047007.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa] >TYK04773.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1228 bits (3178), Expect = 0.0
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK
Sbjct: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW
Sbjct: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 604
           SQQD
Sbjct: 601 SQQD 604

BLAST of IVF0013545 vs. NCBI nr
Match: XP_004149936.1 (indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN56171.1 hypothetical protein Csa_010792 [Cucumis sativus])

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 587/604 (97.19%), Postives = 597/604 (98.84%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPKPST NYNQL+QKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP
Sbjct: 1   MPEAPKPSTQNYNQLLQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           S DTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SSDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           EL+RRSLLYSLLMPVM+QFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELDRRSLLYSLLMPVMTQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK
Sbjct: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQAVRDAVM SVLR PDPELADY+HGECCKG WQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMSSVLRGPDPELADYVHGECCKGWWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYITTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLP+ERSH NDDNSL+EQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPVERSHINDDNSLNEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+N+L+PFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNNLMPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWE+NQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWELNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW
Sbjct: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 604
           SQQD
Sbjct: 601 SQQD 604

BLAST of IVF0013545 vs. NCBI nr
Match: XP_038905526.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1173 bits (3034), Expect = 0.0
Identity = 576/604 (95.36%), Postives = 591/604 (97.85%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60
           MPEAPK +TPNYN LVQKNKAILQFIEDVTTNAALVQRQVLSQILS+NSNSEYLTLYGRP
Sbjct: 1   MPEAPKTATPNYN-LVQKNKAILQFIEDVTTNAALVQRQVLSQILSRNSNSEYLTLYGRP 60

Query: 61  SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120
           SPDTFKTSIPLVSY+QIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE
Sbjct: 61  SPDTFKTSIPLVSYEQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 ELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFK 180
           ELERRSLLYSLLMPVM+QFVPGLEKGKGMYFLF+KAEAKTPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELERRSLLYSLLMPVMTQFVPGLEKGKGMYFLFVKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 ERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEK 240
           +RPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRL+VLRVGAVFASGFIRAIRFLEK
Sbjct: 181 DRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLNVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLW 300
           HWQLLCHDIRTGTLNSQITDQ VRDAVML VLRRPDPELADY+HGECCKGSWQGIITRLW
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQEVRDAVML-VLRRPDPELADYVHGECCKGSWQGIITRLW 300

Query: 301 PNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360
           PNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLI 360

Query: 361 PSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420
           PSMAYFEFLP+ER+H  D+ SLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV
Sbjct: 361 PSMAYFEFLPVERNHKGDNKSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 420

Query: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTS 480
           AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS NHLVPFEATLAEYTSYANTS
Sbjct: 421 AGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSANHLVPFEATLAEYTSYANTS 480

Query: 481 TIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540
           TIPGHYVLYWE+NQK G+ TPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV
Sbjct: 481 TIPGHYVLYWELNQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVV 540

Query: 541 ENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQW 600
           E GTFDKLMDYAISMGASINQYKTPRCVKFQPIVELL+SRV+ SYFSPKCPKWVPGHKQW
Sbjct: 541 ETGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLDSRVLASYFSPKCPKWVPGHKQW 600

Query: 601 SQQD 604
           SQQD
Sbjct: 601 SQQD 602

BLAST of IVF0013545 vs. NCBI nr
Match: XP_022985190.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1129 bits (2921), Expect = 0.0
Identity = 557/607 (91.76%), Postives = 586/607 (96.54%), Query Frame = 0

Query: 1   MPEAPKPST---PNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLY 60
           MPEAPK +T   PNYN LVQKN AILQFIEDVTTNA LVQRQVLSQILS+N+ SEYL+L+
Sbjct: 1   MPEAPKTATSVSPNYN-LVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLH 60

Query: 61  GRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPT 120
           GRPSPDTFK+SIPLVSYD IQPF+SRIANGD SPILCSSPISEFLTSSGTSGGERKLMPT
Sbjct: 61  GRPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSS 180
           IEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIIT 300
           LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML VLRRPDPELAD+IH +CCKG W+GIIT
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML-VLRRPDPELADFIHEQCCKGFWKGIIT 300

Query: 301 RLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360
           RLWPNTKY+DVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY
Sbjct: 301 RLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360

Query: 361 TLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           TLIPSMAYFEFLP++R+ NND+ SLD+QQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI
Sbjct: 361 TLIPSMAYFEFLPVQRTSNNDE-SLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420

Query: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYA 480
           LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK S+NHLVPFEATLAEYTS+A
Sbjct: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAEYTSHA 480

Query: 481 NTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEI 540
           NTSTIPGHYVLYWE+NQK G+ATPVPPSVLEDCCLTIEESLNSVYRQGRV+DKSIGPLEI
Sbjct: 481 NTSTIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEI 540

Query: 541 KVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGH 600
           KVVE+GTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVG YFSPKCPKWV GH
Sbjct: 541 KVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGH 600

Query: 601 KQWSQQD 604
           KQWSQQD
Sbjct: 601 KQWSQQD 604

BLAST of IVF0013545 vs. NCBI nr
Match: XP_023520418.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1129 bits (2921), Expect = 0.0
Identity = 557/607 (91.76%), Postives = 585/607 (96.38%), Query Frame = 0

Query: 1   MPEAPKPST---PNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLY 60
           MPEAPK  T   PNYN LVQKN AILQFIEDVTTNA LVQRQVLSQILS+N+ SEYL+L+
Sbjct: 1   MPEAPKTPTSVSPNYN-LVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLH 60

Query: 61  GRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPT 120
           GRPSPDTFK+SIPLVSYD IQPF+SRIANGD SPILCSSPISEFLTSSGTSGGERKLMPT
Sbjct: 61  GRPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 IEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSS 180
           IEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRF 240
           HFK RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGLC+RLDVLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIIT 300
           LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML VLRRPDPELAD+IH +CCKG W+GIIT
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVML-VLRRPDPELADFIHEQCCKGYWKGIIT 300

Query: 301 RLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360
           RLWPNTKY+DVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY
Sbjct: 301 RLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAY 360

Query: 361 TLIPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           TLIPSMAYFEFLP++R+ NND+ SLD+QQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI
Sbjct: 361 TLIPSMAYFEFLPVQRTSNNDE-SLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420

Query: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYA 480
           LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFEATLAEYTS+A
Sbjct: 421 LRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSHA 480

Query: 481 NTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEI 540
           NTSTIPGHYVLYWE+NQK G+ TPVPPSVLEDCCLTIEESLNSVYRQGRV+DKSIGPLEI
Sbjct: 481 NTSTIPGHYVLYWELNQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEI 540

Query: 541 KVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGH 600
           KVVE+GTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVG YFSPKCPKWV GH
Sbjct: 541 KVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGH 600

Query: 601 KQWSQQD 604
           KQWSQQD
Sbjct: 601 KQWSQQD 604

BLAST of IVF0013545 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 948.0 bits (2449), Expect = 3.8e-276
Identity = 468/613 (76.35%), Postives = 529/613 (86.30%), Query Frame = 0

Query: 1   MPEAPKPSTPNYN--QLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLT--- 60
           MPEAPK +    +   L +KNK  LQFIEDVTTNA  VQR+VL +ILS+N++ EYL    
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  LYGRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLM 120
           L GR   +TFK  +P+V+Y+ IQP ++RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVM QFVPGL+KGKGMYFLFIK+E+KTPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK RPYDPYTNYTSPN+ ILC DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGI 300
           +FLEKHW  L  DIRTGTL+S+ITD +VR+AV    + +PDP+LAD++  EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPIERSHNNDDN--------SLDEQQLVDLTDVELGKEYELVVTTYA 420
           +YTLIP+MAYFEFLP+ R+     +          ++Q+LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFE 480
           GLYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN++ HLVPF+
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLAEYTSYANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRV 540
           A+L+EYTSYA+TS+IPGHYVL+WE+       TP+PPSV EDCCLTIEESLNSVYRQGRV
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCL--NGNTPIPPSVFEDCCLTIEESLNSVYRQGRV 540

Query: 541 SDKSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFS 600
           SDKSIGPLEIK+VE+GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV SYFS
Sbjct: 541 SDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFS 600

BLAST of IVF0013545 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 939.1 bits (2426), Expect = 1.8e-273
Identity = 467/614 (76.06%), Postives = 527/614 (85.83%), Query Frame = 0

Query: 1   MPEAPKPSTPNYNQLV--QKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYL---T 60
           MPEAPK  +     L   QKNK  LQ IE++T+NA  VQRQVL +IL++N++ EYL    
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  LYGRPSPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLM 120
           L GR   +TFK  +P+++Y+ I+P ++RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVMSQFVPGLE GKGMYFLFIK+E+KTPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFKERPYDPYTNYTSPNE ILC DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGI 300
           +FLEKHW  L  DIRTGTL+S ITD +VR+AV  + + +P P+LAD++  EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPIERSHNNDDNSL---------DEQQLVDLTDVELGKEYELVVTTY 420
           +YTLIPSMAYFEFLP+ R +N   NS+         ++Q+LVDL DV+LG+EYELVVTTY
Sbjct: 361 SYTLIPSMAYFEFLPVHR-NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 420

Query: 421 AGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPF 480
           AGL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN++ HLVPF
Sbjct: 421 AGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 480

Query: 481 EATLAEYTSYANTSTIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGR 540
           +A+L+EYTSYA+TS+IPGHYVL+WE+       TP+PPSV EDCCL +EES N+VYRQGR
Sbjct: 481 DASLSEYTSYADTSSIPGHYVLFWELCL--DGNTPIPPSVFEDCCLAVEESFNTVYRQGR 540

Query: 541 VSDKSIGPLEIKVVENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYF 600
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV SYF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

BLAST of IVF0013545 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 777.3 bits (2006), Expect = 8.8e-225
Identity = 384/596 (64.43%), Postives = 467/596 (78.36%), Query Frame = 0

Query: 8   STPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYL---TLYGRPSPDT 67
           S+P      +K+   L+FIE++T NA  VQ  +L++IL++N+++EYL    L G    DT
Sbjct: 8   SSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDT 67

Query: 68  FKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELER 127
           FKT IP+++Y+ +QP + RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+R
Sbjct: 68  FKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDR 127

Query: 128 RSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPY 187
           R LLYSLLMPVM+ +VPGL+KGKGMYFLF+K+E KTPGGL ARPVLTSYYKS HF+ RPY
Sbjct: 128 RQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPY 187

Query: 188 DPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 247
           DPY  YTSPNEAILCPDS+QSMY Q+LCGL  RL VLRVGAVFASG +RAIRFL+ HW  
Sbjct: 188 DPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSR 247

Query: 248 LCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTK 307
             HDI  G L+S+ITD ++R    +S + +PDP LA++I  EC   +W+ IITR+WPNTK
Sbjct: 248 FAHDIELGCLDSEITDPSIRQC--MSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTK 307

Query: 308 YVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMA 367
           Y+DVIVTG M+QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEV+YT++P+MA
Sbjct: 308 YLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMA 367

Query: 368 YFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFK 427
           YFEF+P           L   + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF 
Sbjct: 368 YFEFIP-----------LGGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 427

Query: 428 NKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHL-VPFEATLAEYTSYANTSTIP 487
           N APQF+F+ RKNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TSTIP
Sbjct: 428 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIP 487

Query: 488 GHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENG 547
           GHYVLYWE+  ++GA  P     L  CCL +EESLNSVYRQ RV+D S+GPLEI+VV NG
Sbjct: 488 GHYVLYWELLVRDGARQP-SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNG 547

Query: 548 TFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600
           TF++LMDYAIS GASINQYK PRCV F PIVELL+SRVV ++FSP  P W P  ++
Sbjct: 548 TFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of IVF0013545 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 772.3 bits (1993), Expect = 2.8e-223
Identity = 377/591 (63.79%), Postives = 468/591 (79.19%), Query Frame = 0

Query: 17  QKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYL---TLYGRPSPDTFKTSIPLVS 76
           +K+   L+FIE++T N   VQ +VL +IL++NSN+EYL    L G     TFK+ +P+V+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 77  YDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 136
           Y+ ++P + RI+NGD SPIL S PI+EFLTSSGTS GERKLMPTIEE+L+RR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 137 PVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDPYTNYTSP 196
           PVM+ +VPGL+KGKG+YFLF+K+E+KT GGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 197 NEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGT 256
           NEAILC DS QSMYAQ+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L  DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 257 LNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYVDVIVTGT 316
           L+S+I D A+++  M  +L +PD ELA+++ G C + +W+GIIT++WPNTKY+DVIVTG 
Sbjct: 258 LSSRIFDPAIKNR-MSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGA 317

Query: 317 MSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPIER 376
           M+QYI TL+YYS GLP+ CTMYASSE YFG+NL P+CKPSEV+YT++P+MAYFEFLP   
Sbjct: 318 MAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--- 377

Query: 377 SHNND-----DNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAP 436
            HN+D     + SLDE  LV+L +VE+GKEYELV+TTYAGLYRYRVGDILRV GF N AP
Sbjct: 378 -HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAP 437

Query: 437 QFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHYVL 496
           QF FI RKNV+LS++SDKTDE ELQ AV+N+          + EYTSYA T TIPGHYV+
Sbjct: 438 QFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVI 497

Query: 497 YWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFDKL 556
           YWE+  ++ +   +   V+  CCL +EESLNSVYRQ RV+DKSIGPLEI+VV NGTF++L
Sbjct: 498 YWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEEL 557

Query: 557 MDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600
           MDYAIS GASINQYK PRCV F PI+ELL+SRVV ++FSP  P W P  ++
Sbjct: 558 MDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERRR 603

BLAST of IVF0013545 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 746.9 bits (1927), Expect = 1.3e-215
Identity = 370/586 (63.14%), Postives = 460/586 (78.50%), Query Frame = 0

Query: 18  KNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLT---LYGRPSPDTFKTSIPLVSY 77
           K+   L+FIE++T N   VQ++V+ +ILS+NS++EYL    L G     TFKT +P+V Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 78  DQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMP 137
           D ++P + RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR LLYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 138 VMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDPYTNYTSPN 197
           VM+ +VPGL+KGK +YFLF+K E+KTPGGL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 198 EAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGTL 257
           EAILCPDS QSMY Q+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L  DI TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 258 NSQITDQAVRDAVMLSVLRRPDPELADYIHGECCK-GSWQGIITRLWPNTKYVDVIVTGT 317
           +S+I+D A++++ M  +L +PD ELAD+I   C +  SW+GIIT++WPNTKY+DVIVTG 
Sbjct: 258 SSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGA 317

Query: 318 MSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPIER 377
           M+QYI  L+YYS GLP+ CTMYASSE YFG+NL P+CKPSEV+YT++P+MAYFEFLP   
Sbjct: 318 MAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--- 377

Query: 378 SHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFI 437
              + +   ++ +LV+L DVE+GKEYELV+TTYAGL RYRVGDIL+V GF N APQF F+
Sbjct: 378 ---HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFV 437

Query: 438 CRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHYVLYWEVN 497
            RKNV+LSI+SDKTDE ELQ+AV+N+   L      + EYTSYA T TIPGHYV+YWE+ 
Sbjct: 438 RRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 498 QKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFDKLMDYAI 557
            K+    P    V+  CCL +EESLNSVYRQ RV+DKSIGPLEI+VV+NGTF++LMDYAI
Sbjct: 498 VKD-QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAI 557

Query: 558 SMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600
           S GASINQYK PRCV F PI+ELL+SRVV ++FSP  P W P  ++
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LSQ45.3e-27576.35Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818292.5e-27276.06Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM55.4e-24368.37Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ62.8e-23166.18Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
O823331.2e-22364.43Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A5D3BYD30.0e+00100.00Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BTN30.0e+00100.00indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493... [more]
A0A0A0L7R70.0e+0097.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G088930 PE=3 SV=1[more]
A0A6J1JAP20.0e+0091.76indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1EW650.0e+0090.77indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
XP_008452341.10.0100.00PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA004700... [more]
XP_004149936.10.097.19indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN56171.1 hypoth... [more]
XP_038905526.10.095.36indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida][more]
XP_022985190.10.091.76indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_023520418.10.091.76indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G54510.13.8e-27676.35Auxin-responsive GH3 family protein [more]
AT4G27260.11.8e-27376.06Auxin-responsive GH3 family protein [more]
AT2G14960.18.8e-22564.43Auxin-responsive GH3 family protein [more]
AT4G37390.12.8e-22363.79Auxin-responsive GH3 family protein [more]
AT2G23170.11.3e-21563.14Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 26..570
e-value: 9.6E-200
score: 664.9
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 10..601
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 10..601

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0013545.1IVF0013545.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity