IVF0012057 (gene) Melon (IVF77) v1

Overview


Name	IVF0012057
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Cytochrome P450 81D1-like
Location	chr11: 8727113 .. 8729531 (-)
RNA-Seq Expression	IVF0012057
Synteny	IVF0012057

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACGTAAATTATGGGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGGTGGATCAACAAATCCAGGGGATTTCATACCTTTATTGAATTGGATTGATCCAAGTGGTTATACGAAAAGGATATAATGAAGATTGGAAGAAAAACAGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGTATGTTTTTCTTTCTAAATTAACATCTAAGTTTGATTTTCCAATATATAAACAATTTTTGTCGATCGTCAAAGAGTAACAATATGATCTTTTCATTTTTTATTTTACACCTTTAGTAATTTCTAAAGAGATTTAAGCTTCTGACTACTTGGGTTTTTGTTTCAGAAGTTTTCCTTGTTTCCCATCATTTATTGCATAGAGTTATAGACTTCTATTAACAGATTTTGCTATATTTACAATTTTTTGAAAATATTACTATATACTTAATTATTTTGAATCTAATTGCTTTATTTCCAACTATCGGTAAAATAAACATTAAAATCTATTTTGAACATTGTTTTCTAAAAACTCCAATTTTCTCTGAAATTAATTCTTTTTTAATTAAAAACTTGAGAATATATTTGAAGAATTTTCCAAACTAACATGCTCATGTATTTGTCATATAAAACTAGCACCTTTTTTAAAAAAATAGTTCAAATAAATTTTTCAATCCATCTCGAACGAGATTGACCACAAAAGTTTTGGTAAAAATTAAAATTTTCTAACTTATTGATTCTTTTTAACCTTCTCAGTACATCTTGGTGGCAATTACATCGAACTTTTAGTAATTTCTCAAATCTTATCAAATCGTATACCAAATCTTATATGTTTTTATGATTTTTCTAAATTTGGCTATTTTGTCGTATATTATGTGCAATTCTTGCATTCTATAGAAATCAATTTAGAGATCTAAACAATTACTAATATATGATATTTTGTAAATATAACTGAATTTAAAAAAGTTGAAAATTATAATGAGGTTTGATATTAGATTTGGGAATATTTTAGAATCTCTGTCTAATCTCTATCGAGATGTAGCCTAAAACAATCAATAACAAACCAAATCTATCCCTCAAATTACTCCATCACTTCTTCCAACGAGTTTTCAACTTTTGCAGGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGA

mRNA sequence

ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGGTGGATCAACAAATCCAGGGGATTTCATACCTTTATTGAATTGGATTGATCCAAGTGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGA

Coding sequence (CDS)

ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGGTGGATCAACAAATCCAGGGGATTTCATACCTTTATTGAATTGGATTGATCCAAGTGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGA

Protein sequence

MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLHEFRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK

Homology

[Close]

BLAST of IVF0012057 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 6.2e-138
Identity = 253/490 (51.63%), Postives = 339/490 (69.18%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYS-FLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTL 60
           M+   LLYT L+++ L +    F     R NLPP+P   LP IGHLHL+   +H++   L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SQKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 120
           S+KYG VFSL+ G+RLV++VSSP+A +ECFTKNDI+ ANRP    GKY+ YN TTMV S 
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDL 180
           YG+HWRNLRR++ +EIFS   LN F   RE+EVK+LL         +F  VE++  L +L
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 TFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID- 240
           +FN+ MRMV GK++      +V  DE   K F+ L+ ++  H G++NPGDF+P L WID 
Sbjct: 181 SFNVTMRMVAGKRYF---GQDVDSDE--AKLFRALIGEVFEHAGASNPGDFVPFLRWIDF 240

Query: 241 ------------PSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 300
                         D  LQ L+ E R  ++   TMI HLL LQ++ PEYY+DQIIKG++ 
Sbjct: 241 KNYEKKVSKISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIM 300

Query: 301 DIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGII 360
            ++LAG DTS +++EWA+S LLN+PE L+KA+ EI+  +G  RL+ E DLP L+YL  II
Sbjct: 301 VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNII 360

Query: 361 SETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPER 420
           SET RL P AP+LVPH    ++CK++ Y++P+ TI+L+NAWAIHRD   W++ T FKPER
Sbjct: 361 SETFRLCPAAPMLVPH-ESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPER 420

Query: 421 HSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGL 469
           H  +E E  KL+PFG+GRR+CPG+G+AQRVVGL L +LIQ FEW+R+ ++ IDM+EG GL
Sbjct: 421 HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGL 480

[Close]

BLAST of IVF0012057 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.1e-134
Identity = 256/490 (52.24%), Postives = 332/490 (67.76%), Query Frame = 0


Query: 8   YTLLSLISLLLGYSFLFK--PHRL-NLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYG 67
           Y+LLSL S ++    L K    RL NLPP P   +P IG+LH LKHP+H+TF TLSQ YG
Sbjct: 5   YSLLSL-SFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYG 64

Query: 68  HVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHW 127
            +FSL FGSRLVV+VSSPS   ECFTKNDIILANRP   +GKY+ YN TT+  +SYG HW
Sbjct: 65  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 124

Query: 128 RNLRRISTLEIFSTTRLNSFSRTREEEVKRLLHE-----------FRVVELEPMLLDLTF 187
           RNLRRI+T+++ S  RLNSF   R +E  RL+ +           F  VEL P L ++TF
Sbjct: 125 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 184

Query: 188 NIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID--- 247
           N +MRM+ GK++  +  +  + D    K+F+E+++++M+  G+ N GDF+PLL  +D   
Sbjct: 185 NAMMRMISGKRYYGDDGD--VSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 244

Query: 248 ----------PSDEVLQGLVDEIR--NEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 307
                      S+  L+GL++E R  N   +G TMI HLL+L ++ PEYYSD +IKGL+Q
Sbjct: 245 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 304

Query: 308 DIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGII 367
            ++LAG DTS +++EW +S+LLN+PEVLKKAKEE+D  IG  +LV E DL  L YLQ II
Sbjct: 305 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 364

Query: 368 SETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPER 427
           SETLRLHPPAPLL+PH +  E+C I  +N+P+DTI+L N W IHRD   W DA  FKPER
Sbjct: 365 SETLRLHPPAPLLLPHYS-SEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER 424

Query: 428 HSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGL 469
               E E+ K++ FG+GRRACPG  +AQR VG  +  LIQ FEWER  +  +DM EGKG+
Sbjct: 425 FEK-EEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGI 484

[Close]

BLAST of IVF0012057 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.2e-134
Identity = 252/488 (51.64%), Postives = 335/488 (68.65%), Query Frame = 0


Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+T ++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ + 
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLHEF--------RVVELEPMLLDL 185
           YG HWRNLRR+ T+EIFST RLN F   R +EV+RL+            VVEL+PML+DL
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 188

Query: 186 TFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI-- 245
           TFN +MRM+ GK++  E+      DE   KR ++LV  + A+  S N  D++P+L     
Sbjct: 189 TFNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFSS 248

Query: 246 ---------DPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDI 305
                    + +D+ LQGL+D+ R +++ G TMI HLL LQK+D EYY+DQIIKG++  +
Sbjct: 249 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 308

Query: 306 ILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISE 365
           ++AG +TS ++LEWALS LLN+P+V+ KA++EID  +G  RL+ E DL  L YL+ I+ E
Sbjct: 309 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 368

Query: 366 TLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHS 425
           TLRLHP  PLLVPH A  E+CKI  Y++PR T +L+NAWAIHRD   W+D   FKPER  
Sbjct: 369 TLRLHPATPLLVPHMA-SEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE 428

Query: 426 VIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTM 469
             E E  KL+ FG+GRRACPG+G+AQR+VGLAL SLIQ FEWERV +  +DM EG G T+
Sbjct: 429 K-EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTV 488

[Close]

BLAST of IVF0012057 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 3.1e-129
Identity = 244/488 (50.00%), Postives = 330/488 (67.62%), Query Frame = 0


Query: 10  LLSLISLLLGYSFLFKPHRL--------NLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQ 69
           +LSL+S  + Y  LF    +        NLPP P   LP IG+LH LK P+H+TF+ LS+
Sbjct: 3   ILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSE 62

Query: 70  KYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYG 129
           KYGHVFSL FGSRLVV+VSS S  Q+CFTKND++LANRP   SGKY+ YN TT+  +SYG
Sbjct: 63  KYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122

Query: 130 KHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDLTF 189
           +HWRNLRRI+ L++ S  R+NSFS  R +E +RL+          F  +EL   L D+TF
Sbjct: 123 EHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTF 182

Query: 190 NIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID--- 249
           N +MRM+ GK++  E  +    D     +F+++V++++   G+ N  DF+PLL ++D   
Sbjct: 183 NNIMRMISGKRYYGEDCDT--SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 242

Query: 250 ----------PSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDI 309
                      +D  L+GL++E R +++  NTMI HLL LQ + PEYY+DQIIKGL   +
Sbjct: 243 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 302

Query: 310 ILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISE 369
           +LAG D+S ++LEW++S LLN+PEVLKK K+E+D  +G  RLV E DLP L+YL+ +I+E
Sbjct: 303 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 362

Query: 370 TLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHS 429
           TLRL+ PAPLL+PH +  +EC I  Y +P+DTIVLINAWAIHRD  LW +AT FKPER  
Sbjct: 363 TLRLYTPAPLLLPH-STSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 422

Query: 430 VIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTM 469
             + EL KLI FG+GRRACPG G+A R + + LA LIQ F+W+ ++   ID++E  G T+
Sbjct: 423 K-KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTL 482

[Close]

BLAST of IVF0012057 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.9e-129
Identity = 247/488 (50.61%), Postives = 334/488 (68.44%), Query Frame = 0


Query: 7   LYTLLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQK 66
           +Y +LSL    + L    LF  +P +LNLPP+P    P IGHLHLLK P+H+ F +LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 67  YGH--VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 126
             +  +FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TTMV ++Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 127 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDLT 186
           G  WRNLRRI T+EIFS+ RLNSF   R++E++RL+        H F  VE++P+ + LT
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLT 190

Query: 187 FNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI--- 246
            N ++RMV GK+F  +      E++   K  ++L+ +++  GG+ N  D+ P+L ++   
Sbjct: 191 INNIIRMVAGKRFYGDGT----ENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTNY 250

Query: 247 --------DPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDII 306
                      DE LQ LV+E R E+ +GNTMI HLL LQ+T P+YY+D IIKG++  +I
Sbjct: 251 EKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMI 310

Query: 307 LAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISET 366
           LAG DTS  +LEWA+S LLN+PEVL+KAK EID  IG  RLV E D+  L YLQ I+SET
Sbjct: 311 LAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSET 370

Query: 367 LRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSV 426
           LRL+P AP+L+PH A  E+C +  Y++PR TI+L+NAWAIHRD  LWE+  +FKPER   
Sbjct: 371 LRLYPVAPMLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 430

Query: 427 IESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG-KGLTM 469
            + E  KL+PFG+GRR+CPG+G+AQR+V LAL SL+Q FEWERV++  +DM E  KG TM
Sbjct: 431 -KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTM 490

[Close]

BLAST of IVF0012057 vs. ExPASy TrEMBL
Match: A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.0e-265
Identity = 468/492 (95.12%), Postives = 468/492 (95.12%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 PS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           PS             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420

Query: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 470
           RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG
Sbjct: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 480

[Close]

BLAST of IVF0012057 vs. ExPASy TrEMBL
Match: A0A5D3BGH3 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00710 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.5e-248
Identity = 446/479 (93.11%), Postives = 446/479 (93.11%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL   IM  G  T             
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL--DIMKIGRKT------------- 240

Query: 241 PSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI 300
             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI
Sbjct: 241 --DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI 300

Query: 301 SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL 360
           SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL
Sbjct: 301 SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL 360

Query: 361 LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI 420
           LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI
Sbjct: 361 LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI 420

Query: 421 PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 470
           PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK
Sbjct: 421 PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 462

[Close]

BLAST of IVF0012057 vs. ExPASy TrEMBL
Match: A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 8.5e-231
Identity = 409/494 (82.79%), Postives = 435/494 (88.06%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           G+HWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 PS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           PS             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDI+LAGIDTS ++L+WALS LLNNP VL+KAK EID  IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRL P  PLLVPHC+  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FKPE
Sbjct: 361 ISETLRLSPAGPLLVPHCS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 470
           RH  +      YKL+PFG+GRRACPG  MAQRV+GL LASL+Q FEW+R+ D L+DM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

[Close]

BLAST of IVF0012057 vs. ExPASy TrEMBL
Match: A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.0e-216
Identity = 390/496 (78.63%), Postives = 421/496 (84.88%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL----------HEFRVVELEPMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL           EFRVVELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
           LT NIVMRMVGGKKFC+E  KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 IDPS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
           IDPS             DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
           GIISETLRL PP PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  +WEDA  FK
Sbjct: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 470
           PERH  +V     YKL+PFG+GRRACPG  MAQRVVGL LASLIQ FEW+++ + L+DM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

[Close]

BLAST of IVF0012057 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.7e-187
Identity = 337/486 (69.34%), Postives = 389/486 (80.04%), Query Frame = 0


Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           LL   L L+SLL  ++FL  PHRLNLPPTPLF LP IGH HLLKHP+H++   LSQK+GH
Sbjct: 5   LLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQKHGH 64

Query: 66  VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWR 125
           V SL+FGSRLVV++SSPSA+QECFTKNDIILANRP L++GKYLAYN+TT+ VS YG+HWR
Sbjct: 65  VISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWR 124

Query: 126 NLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDLTFNIVM 185
           NLRRI +LEIFST RLN F   RE EVKRLL         EFR VELEPML DLTFN+VM
Sbjct: 125 NLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTFNVVM 184

Query: 186 RMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPS----- 245
            MVGG++F          DEGY K+F++LVTQIMAHGGS+NPGDFIPL NW+DP+     
Sbjct: 185 GMVGGRRF---------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 244

Query: 246 --------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAG 305
                   DE+LQ L+DE R+ +D GNTMI HLL LQK +PEYYSD IIKGL+Q I LAG
Sbjct: 245 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAG 304

Query: 306 IDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRL 365
           IDTS ++LEWALS LLNNP+VLKKA+ EID LIG +RLV+E DLP+L YLQGIISETLRL
Sbjct: 305 IDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRL 364

Query: 366 HPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERH-SVIE 425
           +P APLLVPHCA F++CKI+ Y++PRDTIVLIN WAIHRD  LWED T FKPERH     
Sbjct: 365 NPAAPLLVPHCA-FQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSG 424

Query: 426 SELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKA 470
           ++ YKL+PFG+GRRACPG GMAQRVVGLALASLIQ FEWERV  SL+DMSEG+GLTMPKA
Sbjct: 425 ADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKA 480

[Close]

BLAST of IVF0012057 vs. NCBI nr
Match: XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 468/492 (95.12%), Postives = 468/492 (95.12%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 PS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           PS             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420

Query: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 469
           RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG
Sbjct: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 480

[Close]

BLAST of IVF0012057 vs. NCBI nr
Match: TYJ98343.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 863 bits (2231), Expect = 4.99e-315
Identity = 446/479 (93.11%), Postives = 446/479 (93.11%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL   IM  G  T             
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL--DIMKIGRKT------------- 240

Query: 241 PSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI 300
             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI
Sbjct: 241 --DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTI 300

Query: 301 SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL 360
           SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL
Sbjct: 301 SLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPL 360

Query: 361 LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI 420
           LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI
Sbjct: 361 LVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLI 420

Query: 421 PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 469
           PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK
Sbjct: 421 PFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 462

[Close]

BLAST of IVF0012057 vs. NCBI nr
Match: KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 805 bits (2079), Expect = 4.73e-291
Identity = 409/494 (82.79%), Postives = 435/494 (88.06%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           G+HWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL           EFRVVELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 PS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           PS             DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDI+LAGIDTS ++L+WALS LLNNP VL+KAK EID  IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRL P  PLLVPHC+  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FKPE
Sbjct: 361 ISETLRLSPAGPLLVPHCSS-EDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 469
           RH  +      YKL+PFG+GRRACPG  MAQRV+GL LASL+Q FEW+R+ D L+DM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

[Close]

BLAST of IVF0012057 vs. NCBI nr
Match: XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])

HSP 1 Score: 757 bits (1955), Expect = 3.56e-272
Identity = 390/496 (78.63%), Postives = 421/496 (84.88%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL----------HEFRVVELEPMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL           EFRVVELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
           LT NIVMRMVGGKKFC+E  KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 IDPS-------------DEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
           IDPS             DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
           GIISETLRL PP PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  +WEDA  FK
Sbjct: 361 GIISETLRLSPPGPLLVPHCAS-EDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 469
           PERH  +V     YKL+PFG+GRRACPG  MAQRVVGL LASLIQ FEW+++ + L+DM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

[Close]

BLAST of IVF0012057 vs. NCBI nr
Match: XP_038902241.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 729 bits (1882), Expect = 4.25e-261
Identity = 375/494 (75.91%), Postives = 410/494 (83.00%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFD LL  LLSL+ LLLGYSFL KPH LNLPPTPLF LPFIGHLHLLKHPVHK FQ LS
Sbjct: 1   MDFDLLLCILLSLVYLLLGYSFLLKPHGLNLPPTPLFCLPFIGHLHLLKHPVHKIFQNLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGS LVVLVSSPSA+QECFTKNDIILANRPSLN GKYLAYNNT+M +S Y
Sbjct: 61  QKYGHVFSLKFGSHLVVLVSSPSAVQECFTKNDIILANRPSLNPGKYLAYNNTSMALSPY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLH----------EFRVVELEPMLLD 180
           G+HWRNLRRISTLEIFS TR N F ++RE+EVKRLL+          EFRVVELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSATRFNLFLKSREDEVKRLLYKLCGNDTLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
           LTFNIVMRMVGGKKFCEE N NV EDEGY KRF+ELVTQIMAHGGSTNPGDFIPL NWID
Sbjct: 181 LTFNIVMRMVGGKKFCEENNKNVSEDEGYSKRFRELVTQIMAHGGSTNPGDFIPLWNWID 240

Query: 241 P-------------SDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           P             SDEVLQGL+DEIRN+EDEG  MIQHLLRLQ TDPEYYSDQ+IKGLV
Sbjct: 241 PTGYNKRLMKLGRRSDEVLQGLIDEIRNQEDEGINMIQHLLRLQNTDPEYYSDQVIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDI+L GIDTS ++LEWALS LLNNP VL++AK EIDC IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDILLGGIDTSAVTLEWALSHLLNNPIVLERAKAEIDCFIGQERMVNEADLSSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRL PP PLL PH A  ++C I  Y++PR+T+VL N WAIHRD  LWEDAT FKPE
Sbjct: 361 ISETLRLSPPGPLL-PHYAS-KDCTIGGYDVPRNTMVLFNIWAIHRDPNLWEDATSFKPE 420

Query: 421 RHS-VIESEL-YKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 469
           RH+ V E E  YKL+PFG+GRRACPG  +AQRV+GL LASLIQ FEW+R   SL+DMSEG
Sbjct: 421 RHANVAEVEYSYKLLPFGLGRRACPGMAIAQRVIGLTLASLIQCFEWKRTSKSLVDMSEG 480

[Close]

BLAST of IVF0012057 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.0e-135
Identity = 252/488 (51.64%), Postives = 335/488 (68.65%), Query Frame = 0


Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+T ++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ + 
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLHEF--------RVVELEPMLLDL 185
           YG HWRNLRR+ T+EIFST RLN F   R +EV+RL+            VVEL+PML+DL
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 188

Query: 186 TFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI-- 245
           TFN +MRM+ GK++  E+      DE   KR ++LV  + A+  S N  D++P+L     
Sbjct: 189 TFNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFSS 248

Query: 246 ---------DPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDI 305
                    + +D+ LQGL+D+ R +++ G TMI HLL LQK+D EYY+DQIIKG++  +
Sbjct: 249 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 308

Query: 306 ILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISE 365
           ++AG +TS ++LEWALS LLN+P+V+ KA++EID  +G  RL+ E DL  L YL+ I+ E
Sbjct: 309 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 368

Query: 366 TLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHS 425
           TLRLHP  PLLVPH A  E+CKI  Y++PR T +L+NAWAIHRD   W+D   FKPER  
Sbjct: 369 TLRLHPATPLLVPHMA-SEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE 428

Query: 426 VIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTM 469
             E E  KL+ FG+GRRACPG+G+AQR+VGLAL SLIQ FEWERV +  +DM EG G T+
Sbjct: 429 K-EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTV 488

[Close]

BLAST of IVF0012057 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 477.6 bits (1228), Expect = 1.1e-134
Identity = 247/491 (50.31%), Postives = 338/491 (68.84%), Query Frame = 0


Query: 1   MDFDHLLYTLLSLISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQT 60
           MD   +L  LLS + L +    L      + NLPP+P + LP IGHLHLLK PVH+TF +
Sbjct: 1   MDLTQIL--LLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHS 60

Query: 61  LSQKYGH--VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMV 120
           +S+  G+  +F L+ G+RLV ++SS S  +ECFTKND++LANRP +   K++ YN T M+
Sbjct: 61  ISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMI 120

Query: 121 VSSYGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRL--------LHEFRVVELEPML 180
            +SYG HWRNLRRI+ +EIFS+ R+++FS  R++E++RL        LH F  VEL+ +L
Sbjct: 121 AASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLL 180

Query: 181 LDLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
            +L FN ++ MV GK++         ED    K  +EL+ +IMA  GS N  D++P +NW
Sbjct: 181 TNLAFNNIIMMVAGKRY----YGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINW 240

Query: 241 I-----------DPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
           +           +  D VLQ LVDE R E+++G T+I HLL  Q+T+PEYY+D IIKG++
Sbjct: 241 VTNFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGII 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
             ++LAG DTS+++LEWA+S LLN+PE+L+KA+ EID  IG  RLV E D+ +L YLQ I
Sbjct: 301 LALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           +SETLRL+P  PLL+PH +  +ECK+  Y++PR T++L N WA+HRD GLWE+   FKPE
Sbjct: 361 VSETLRLYPAVPLLLPHFS-SDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPE 420

Query: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 469
           R    E E  KL+PFG+GRRACPGA + +R+V LAL  LIQSFEWERV   L+DM+EG+G
Sbjct: 421 RFEK-EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEG 480

[Close]

BLAST of IVF0012057 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 474.9 bits (1221), Expect = 7.3e-134
Identity = 246/463 (53.13%), Postives = 326/463 (70.41%), Query Frame = 0


Query: 28  RLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH--VFSLKFGSRLVVLVSSPSAI 87
           +LNLPP+P + LP IGHL LLK P+H+ F ++SQ  G   + SL+ G+RL+ +VSS S  
Sbjct: 27  KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIA 86

Query: 88  QECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNLRRISTLEIFSTTRLNSFS 147
           +ECFTKND+ILANR +  S K+++Y N+T+V +SY +HWRNLRRI  LEIFS  RLNSFS
Sbjct: 87  EECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFS 146

Query: 148 RTREEEVKRLL--------HEFRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNNNVLEDE 207
             R +E++RL+        + F  VE++ M  DLTFN ++RM+ GK +  +      ED+
Sbjct: 147 SIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGK----EDD 206

Query: 208 GYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPS-----------DEVLQGLVDEIR-N 267
              KR + L+ + M+  G  N  D+IP+L WI  S           DE LQGLVDE R  
Sbjct: 207 PEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYSETRIKKLAGRLDEFLQGLVDEKREG 266

Query: 268 EEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNNPEV 327
           +E + NTM+ HLL LQ+T PEYY D+IIKG +  +I  G DT+ ++LEWALS LLNNPEV
Sbjct: 267 KEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEV 326

Query: 328 LKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECKIKR 387
           L KA++EID +IG  RL+ E D+P+L YLQ I+SETLRL+P AP+L+PH A  ++CK+  
Sbjct: 327 LNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVA-SKDCKVGG 386

Query: 388 YNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGAGMA 447
           Y++PR T++L NAWAIHRD  LW+D T FKPER    E E  KL+PFG+GRRACPG+G+A
Sbjct: 387 YDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK-EGEAKKLMPFGLGRRACPGSGLA 446

Query: 448 QRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIA 469
           QR+V L+L SLIQ FEWER+ +  +DM+EG GLTMPKA+PL A
Sbjct: 447 QRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEA 483

[Close]

BLAST of IVF0012057 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.5e-131
Identity = 246/484 (50.83%), Postives = 339/484 (70.04%), Query Frame = 0


Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +L+ +LSLI L+       KP   NLPP+P + LP IGHL LLK P+H+TF +LSQ   +
Sbjct: 10  ILFVVLSLIYLI--GKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 69

Query: 66  --VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKH 125
             +FSL+ G+RLV + SS S  +ECFTKND++LANRP+    K++AY+ TTM+ +SYG H
Sbjct: 70  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 129

Query: 126 WRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDLTFNI 185
           WRNLRRI ++EIFS  RLNSF   R++E++RL+         EF  V+++ ML DLTFN 
Sbjct: 130 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNN 189

Query: 186 VMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDP---- 245
           ++RMV GK++      + +ED+   KR ++L+  ++A  G+ N  D++P+L  +      
Sbjct: 190 ILRMVAGKRY----YGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDYETR 249

Query: 246 -------SDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAG 305
                   DE LQGLVDE R  +++GNTMI HLL LQ++ P+Y++D+IIKG +  +ILAG
Sbjct: 250 VKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 309

Query: 306 IDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRL 365
            DTS ++LEWALS +LN+P+VL KA++EID  IG  RL+ E D+ +L YLQ I+SETLRL
Sbjct: 310 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 369

Query: 366 HPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIES 425
           +P AP+L+PH A  E+CK+  Y++PR TI+L N WAIHRD  LW+D   FKPER    E 
Sbjct: 370 YPAAPMLLPHVA-SEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK-EG 429

Query: 426 ELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQ 469
           E  KL+PFG+GRRACPG+G+A R++ L L SLIQ  EWE++ +  +DMSEGKG+TMPKA+
Sbjct: 430 EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAK 481

[Close]

BLAST of IVF0012057 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 462.2 bits (1188), Expect = 4.9e-130
Identity = 247/488 (50.61%), Postives = 334/488 (68.44%), Query Frame = 0


Query: 7   LYTLLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQK 66
           +Y +LSL    + L    LF  +P +LNLPP+P    P IGHLHLLK P+H+ F +LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 67  YGH--VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 126
             +  +FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TTMV ++Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 127 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLL--------HEFRVVELEPMLLDLT 186
           G  WRNLRRI T+EIFS+ RLNSF   R++E++RL+        H F  VE++P+ + LT
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLT 190

Query: 187 FNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI--- 246
            N ++RMV GK+F  +      E++   K  ++L+ +++  GG+ N  D+ P+L ++   
Sbjct: 191 INNIIRMVAGKRFYGDGT----ENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTNY 250

Query: 247 --------DPSDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDII 306
                      DE LQ LV+E R E+ +GNTMI HLL LQ+T P+YY+D IIKG++  +I
Sbjct: 251 EKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMI 310

Query: 307 LAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISET 366
           LAG DTS  +LEWA+S LLN+PEVL+KAK EID  IG  RLV E D+  L YLQ I+SET
Sbjct: 311 LAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSET 370

Query: 367 LRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSV 426
           LRL+P AP+L+PH A  E+C +  Y++PR TI+L+NAWAIHRD  LWE+  +FKPER   
Sbjct: 371 LRLYPVAPMLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 430

Query: 427 IESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG-KGLTM 469
            + E  KL+PFG+GRR+CPG+G+AQR+V LAL SL+Q FEWERV++  +DM E  KG TM
Sbjct: 431 -KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTM 490

The following BLAST results are available for this feature:

BLAST of IVF0012057 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	6.2e-138	51.63	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	1.1e-134	52.24	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9FG65	4.2e-134	51.64	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
P93147	3.1e-129	50.00	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q9LHA1	6.9e-129	50.61	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]

BLAST of IVF0012057 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C1S2	4.0e-265	95.12	LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L...	[more]
A0A5D3BGH3	4.5e-248	93.11	Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7SPU0	8.5e-231	82.79	Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A0A0LQI5	2.0e-216	78.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
A0A6J1G5P7	5.7e-187	69.34	cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1	[more]

BLAST of IVF0012057 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455822.2	0.0	95.12	PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo]	[more]
TYJ98343.1	4.99e-315	93.11	isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa]	[more]
KAA0032538.1	4.73e-291	82.79	isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa]	[more]
XP_004151924.2	3.56e-272	78.63	cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022...	[more]
XP_038902241.1	4.25e-261	75.91	cytochrome P450 81Q32-like [Benincasa hispida]	[more]

BLAST of IVF0012057 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36220.1	3.0e-135	51.64	cytochrome p450 81d1	[more]
AT4G37320.1	1.1e-134	50.31	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37360.1	7.3e-134	53.13	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37370.1	1.5e-131	50.83	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT3G28740.1	4.9e-130	50.61	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 410..419 score: 58.53 coord: 283..300 score: 36.77 coord: 419..430 score: 29.12 coord: 336..347 score: 40.74
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 32..463 e-value: 1.5E-81 score: 274.5
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 409..419 score: 55.07 coord: 272..289 score: 30.99 coord: 419..442 score: 33.88 coord: 84..105 score: 21.19 coord: 171..189 score: 20.77 coord: 292..318 score: 41.63 coord: 60..79 score: 39.83 coord: 377..401 score: 29.85 coord: 335..353 score: 35.67
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..467 e-value: 6.5E-112 score: 376.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..467
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 7..448 e-value: 6.8E-15 score: 52.1
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 7..468
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 7..468
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 412..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0012057.1	IVF0012057.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen