IVF0011578 (gene) Melon (IVF77) v1

Overview


Name	IVF0011578
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Calcium-binding EF-hand family protein
Location	chr02: 20248362 .. 20249497 (+)
RNA-Seq Expression	IVF0011578
Synteny	IVF0011578

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAGTTTACCCCTTCGTTTTCCTCTCCATCGATTTCTCTCTAATTCTGACCTCCAAAAATGGCCACTGACCCTAACCAGATTCCCACCTCACAATCCGACGCCGACCAAATCGCCAAGCAGAAGCCATCCGTTTTACTCGAGGACGACGACGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCTATCACCGAGCTCGATGCCGTCCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGTTATATCAACATCGAAGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAGGCTGGAGCAGCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCTGAGCTCCATCTCGTTCTTAATCGCCTCGGAATCAGTTGTTCCAAGGAAGACTGTCAAAAAATGATCAACTCCGTCGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACAGATAATTCCAAATCGAAGGCGGCTAAACAGAACGGAACTGCTGCGGCGGCACCCTAGCCCTAGCTCTAGGTTTTGGTAAGTTGTTTAGTAATTAGCCGCGTGAGAGAGGTAACGAAGTCGATTTCTCTATCCTTCGTTCCTTTTTTTTTCTTTAATTTCTCTATGTTCTATCCCTTAATCTGCATCCAATCCAACTATTCCTGTTTTCTTATTTTGCCGAAATGTAACAAAAAATTCCGGATCTGCTTCTGTTAATCATCCGCCATTAATGCTGATCTTTAATTTCAACTCTTCTTCATTTCGTTTTCCGATTGAAGATAGAAAAGAAACTTTCCCGGAAAATCGATTGATCTTGCCGGATTCATAATGTGCATTGCTGTTGAGTGATGAGTACATTTCATCCCTATTTCTCCCCCTAAAATTAGAACAATAACAATAAATGAAATAAAACAAAATAAAAAAGAAAGGGGATTTTTTTTTCCCCGAATATTTAAAGGGGAGAAATTGAACATTCTCTACTTAAGAGCTAAACTTATCTGTAGGCCTGAGATCCTCCACTTGTCACC

mRNA sequence

CAAAAGTTTACCCCTTCGTTTTCCTCTCCATCGATTTCTCTCTAATTCTGACCTCCAAAAATGGCCACTGACCCTAACCAGATTCCCACCTCACAATCCGACGCCGACCAAATCGCCAAGCAGAAGCCATCCGTTTTACTCGAGGACGACGACGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCTATCACCGAGCTCGATGCCGTCCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGTTATATCAACATCGAAGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAGGCTGGAGCAGCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCTGAGCTCCATCTCGTTCTTAATCGCCTCGGAATCAGTTGTTCCAAGGAAGACTGTCAAAAAATGATCAACTCCGTCGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACAGATAATTCCAAATCGAAGGCGGCTAAACAGAACGGAACTGCTGCGGCGGCACCCTAGCCCTAGCTCTAGGTTTTGGTAAGTTGTTTAGTAATTAGCCGCGTGAGAGAGGTAACGAAGTCGATTTCTCTATCCTTCGTTCCTTTTTTTTTCTTTAATTTCTCTATGTTCTATCCCTTAATCTGCATCCAATCCAACTATTCCTGTTTTCTTATTTTGCCGAAATGTAACAAAAAATTCCGGATCTGCTTCTGTTAATCATCCGCCATTAATGCTGATCTTTAATTTCAACTCTTCTTCATTTCGTTTTCCGATTGAAGATAGAAAAGAAACTTTCCCGGAAAATCGATTGATCTTGCCGGATTCATAATGTGCATTGCTGTTGAGTGATGAGTACATTTCATCCCTATTTCTCCCCCTAAAATTAGAACAATAACAATAAATGAAATAAAACAAAATAAAAAAGAAAGGGGATTTTTTTTTCCCCGAATATTTAAAGGGGAGAAATTGAACATTCTCTACTTAAGAGCTAAACTTATCTGTAGGCCTGAGATCCTCCACTTGTCACC

Coding sequence (CDS)

ATGGCCACTGACCCTAACCAGATTCCCACCTCACAATCCGACGCCGACCAAATCGCCAAGCAGAAGCCATCCGTTTTACTCGAGGACGACGACGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCTATCACCGAGCTCGATGCCGTCCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGTTATATCAACATCGAAGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAGGCTGGAGCAGCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCTGAGCTCCATCTCGTTCTTAATCGCCTCGGAATCAGTTGTTCCAAGGAAGACTGTCAAAAAATGATCAACTCCGTCGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACAGATAATTCCAAATCGAAGGCGGCTAAACAGAACGGAACTGCTGCGGCGGCACCCTAG

Protein sequence

MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQNGTAAAAP

Homology

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BLAST of IVF0011578 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.3e-42
Identity = 91/170 (53.53%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQKPSVL-LEDDDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + +EL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN++EF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of IVF0011578 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 4.5e-39
Identity = 85/152 (55.92%), Postives = 110/152 (72.37%), Query Frame = 0


Query: 30  DDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIE 89
           D EL+KVF++FDANGDGKIS++EL  V  S  + ++   EEL  V+D++D D DG+IN E
Sbjct: 18  DMELKKVFDKFDANGDGKISVSELGNVFKS--MGTSYTEEELNRVLDEIDIDCDGFINQE 77

Query: 90  EFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 149
           EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS EDC +MI
Sbjct: 78  EFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMI 137

Query: 150 NSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQN 182
             VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 138 GHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

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BLAST of IVF0011578 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-37
Identity = 87/155 (56.13%), Postives = 112/155 (72.26%), Query Frame = 0


Query: 22  KPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSD 81
           KPSV L++ +E++ VF RFDANGDGKIS  EL  VL +  L S    EE+  +M+++D+D
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKA--LGSNTSKEEIGRIMEEIDTD 69

Query: 82  KDGYINIEEFAAFCK---KPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGI 141
           KDG+IN++EFAAF K    P  S   G  EL++AF+LYDQD NGLIS  ELH +L RLG 
Sbjct: 70  KDGFINVQEFAAFVKAETDPYPS-SGGENELKEAFELYDQDHNGLISSVELHKILTRLGE 129

Query: 142 SCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNS 174
             ++ DC +MI SVDSDGDG V+FEEF+KMMT+ S
Sbjct: 130 RYAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKS 161

[Close]

BLAST of IVF0011578 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.1e-32
Identity = 76/149 (51.01%), Postives = 100/149 (67.11%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIEEF 91
           EL +VF R+DANGDGKIS  EL +VL +  L + +   E+R +MD++DSD+DG++++ EF
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRA--LGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 64

Query: 92  AAFCKKPMAS-----------DEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISC 151
           AAF   P  +           + A  AELR+AF +YD D NG IS  ELH VL +LG  C
Sbjct: 65  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 124

Query: 152 SKEDCQKMINSVDSDGDGNVNFEEFRKMM 170
           S  DC +MI SVD+DGDG VNF+EF+KMM
Sbjct: 125 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of IVF0011578 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.0e-32
Identity = 72/146 (49.32%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 31  DELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIEE 90
           ++++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I+++E
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of IVF0011578 vs. ExPASy TrEMBL
Match: A0A5D3DXJ8 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001210 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-96
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of IVF0011578 vs. ExPASy TrEMBL
Match: A0A1S3BX51 (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-96
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of IVF0011578 vs. ExPASy TrEMBL
Match: A0A0A0LPP8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.1e-92
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of IVF0011578 vs. ExPASy TrEMBL
Match: A0A6J1F164 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111441185 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.4e-72
Identity = 142/188 (75.53%), Postives = 168/188 (89.36%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATD NQI  S +D +Q+ K KPS LL+D+ ELRKVF+RFDANGDGKIS++ELD+VL S 
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L S++P +ELR+VM+DLD+DKDG+INI+EFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  -LGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 189
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of IVF0011578 vs. ExPASy TrEMBL
Match: A0A6J1KUB4 (probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC111497259 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.6e-71
Identity = 140/188 (74.47%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATD NQI  S SD +Q+ K KPS LL+D+ ELRKVF+RFDANGDGKIS++ELD+VL S 
Sbjct: 1   MATDLNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S++P +ELR+VM+DLD+DKDG+INI+EFA FCK+P+AS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  -SGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPIASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+   Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEVPNQ 180

Query: 181 NGTAAAAP 189
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of IVF0011578 vs. NCBI nr
Match: XP_008453475.1 (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0058132.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK28486.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 362 bits (928), Expect = 9.16e-126
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 188
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of IVF0011578 vs. NCBI nr
Match: XP_004137340.1 (probable calcium-binding protein CML27 [Cucumis sativus] >KGN63870.1 hypothetical protein Csa_013908 [Cucumis sativus])

HSP 1 Score: 347 bits (891), Expect = 4.01e-120
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 188
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of IVF0011578 vs. NCBI nr
Match: XP_038878467.1 (probable calcium-binding protein CML27 [Benincasa hispida])

HSP 1 Score: 309 bits (792), Expect = 4.60e-105
Identity = 160/188 (85.11%), Postives = 174/188 (92.55%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATDPNQIP S SDADQ++K KPSV L+D++ELRKVFERFDANGDGKISI+ELD+VL SL
Sbjct: 1   MATDPNQIPASNSDADQVSKPKPSVFLQDNEELRKVFERFDANGDGKISISELDSVLKSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S+IP EELRSVM+DLDSDKDGYINI+EFAAFCK+PMASDEAGA+ELRDAFDLYDQD 
Sbjct: 61  G--SSIPYEELRSVMEDLDSDKDGYINIDEFAAFCKEPMASDEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLIS SELHLVLNRLGISCSKEDCQ+MI+SVDSDGDGNVNFEEFRKMMT NS SKAA Q
Sbjct: 121 NGLISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTANSNSKAANQ 180

Query: 181 NGTAAAAP 188
           NGTAAAAP
Sbjct: 181 NGTAAAAP 186

[Close]

BLAST of IVF0011578 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282 bits (722), Expect = 2.12e-94
Identity = 143/188 (76.06%), Postives = 168/188 (89.36%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATD NQI  S SD +Q+ K KPS LL+D+ ELRKVF+RFDANGDGKIS++ELD+VL SL
Sbjct: 1   MATDSNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S++P +ELR+VM+DLD+DKDG+INI+EFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  G--SSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 188
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of IVF0011578 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281 bits (719), Expect = 6.07e-94
Identity = 142/188 (75.53%), Postives = 168/188 (89.36%), Query Frame = 0


Query: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60
           MATD NQI  S +D +Q+ K KPS LL+D+ ELRKVF+RFDANGDGKIS++ELD+VL SL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S++P +ELR+VM+DLD+DKDG+INI+EFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  G--SSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 188
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of IVF0011578 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-43
Identity = 91/170 (53.53%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQKPSVL-LEDDDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + +EL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN++EF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of IVF0011578 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-43
Identity = 91/170 (53.53%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQKPSVL-LEDDDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + +EL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN++EF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of IVF0011578 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 162.5 bits (410), Expect = 3.2e-40
Identity = 85/152 (55.92%), Postives = 110/152 (72.37%), Query Frame = 0


Query: 30  DDELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIE 89
           D EL+KVF++FDANGDGKIS++EL  V  S  + ++   EEL  V+D++D D DG+IN E
Sbjct: 18  DMELKKVFDKFDANGDGKISVSELGNVFKS--MGTSYTEEELNRVLDEIDIDCDGFINQE 77

Query: 90  EFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 149
           EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS EDC +MI
Sbjct: 78  EFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMI 137

Query: 150 NSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQN 182
             VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 138 GHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]

BLAST of IVF0011578 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 138.7 bits (348), Expect = 5.0e-33
Identity = 72/146 (49.32%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 31  DELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIEE 90
           ++++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I+++E
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of IVF0011578 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 72/143 (50.35%), Postives = 90/143 (62.94%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISITELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIEEF 91
           EL  VF++FD NGDGKIS  EL A++TS  L   +P EEL   + ++D   DGYIN EEF
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTS--LGHEVPEEELEKAITEIDRKGDGYINFEEF 96

Query: 92  AAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINS 151
                K M  ++     L+DAF +YD D NG IS  ELH VL  LG  CS  +C+KMI  
Sbjct: 97  VELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 156

Query: 152 VDSDGDGNVNFEEFRKMMTDNSK 175
           VD DGDG ++FEEF+ MMT  S+
Sbjct: 157 VDKDGDGTIDFEEFKIMMTMGSR 176

The following BLAST results are available for this feature:

BLAST of IVF0011578 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE22	3.3e-42	53.53	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9C9U8	4.5e-39	55.92	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]
Q9M7R0	1.1e-37	56.13	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q0DJV6	4.1e-32	51.01	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	7.0e-32	49.32	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of IVF0011578 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DXJ8	1.1e-96	100.00	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BX51	1.1e-96	100.00	probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 P...	[more]
A0A0A0LPP8	2.1e-92	95.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1	[more]
A0A6J1F164	1.4e-72	75.53	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KUB4	4.6e-71	74.47	probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC1114972...	[more]

BLAST of IVF0011578 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453475.1	9.16e-126	100.00	PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0058132.1 p...	[more]
XP_004137340.1	4.01e-120	95.21	probable calcium-binding protein CML27 [Cucumis sativus] >KGN63870.1 hypothetica...	[more]
XP_038878467.1	4.60e-105	85.11	probable calcium-binding protein CML27 [Benincasa hispida]	[more]
XP_023529417.1	2.12e-94	76.06	probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo]	[more]
XP_022933917.1	6.07e-94	75.53	probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putati...	[more]

BLAST of IVF0011578 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18210.1	2.4e-43	53.53	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	2.4e-43	53.53	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	3.2e-40	55.92	EF hand calcium-binding protein family	[more]
AT1G66400.1	5.0e-33	49.32	calmodulin like 23	[more]
AT1G24620.1	1.0e-30	50.35	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 70..98 e-value: 8.6E-5 score: 31.9 coord: 32..60 e-value: 2.1E-5 score: 33.9 coord: 108..136 e-value: 2.1E-4 score: 30.7 coord: 144..172 e-value: 4.5E-5 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 108..169 e-value: 5.5E-14 score: 52.4 coord: 31..95 e-value: 1.1E-12 score: 48.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 28..63 score: 15.378162
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 12.504936
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 104..139 score: 13.09074
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 140..175 score: 14.06708
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 108..170 e-value: 2.19363E-20 score: 78.3585
None	No IPR available	GENE3D	1.10.238.10		coord: 104..183 e-value: 1.1E-23 score: 85.3
None	No IPR available	GENE3D	1.10.238.10		coord: 3..103 e-value: 1.4E-20 score: 75.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	PANTHER	PTHR10891:SF814	CALCIUM-BINDING PROTEIN CML26-RELATED	coord: 15..173
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 117..129
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 41..53
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 153..165
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 29..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0011578.2	IVF0011578.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding