IVF0007865 (gene) Melon (IVF77) v1

Overview
NameIVF0007865
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr03: 14241097 .. 14242987 (-)
RNA-Seq ExpressionIVF0007865
SyntenyIVF0007865
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGAACTATGCAAGTCATTCTCCTTCTAGCCCTAGTCTTTCTGTTTTCATCTCTCTCGACTTATTCCTTACCCTTGTCAACACGAGGAAGATGGATTATTGATTCGAAAACAGGACAACGAGTGAAACTCGTGTGTGTAAATTGGCCTTCTCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTGAAAGAACTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACGCACATGTTCACACGCTACGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGGTCAAGCAAAAGCTGGATTGACTCAATACAATCCTTTTGTCTTGAACAAGACTATTGCAGAAGCTTATGAAGCTGTTGTTGATGTATTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTATGTAACATCAAAAGATCTTCTTATCAATTTATTCAAACTAAATTACTAAAGGATTTTAAAATTTCTATTATCTTTTCATTAAACAATGAAAATCTTTTTATGATTGTTTAGGTTGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACCACTATGTAACTCAAGGAGTAACCACGATCCATAACATAAATCCAGAAGTTTTAGTGATTGTTGGAGGCTTAAATTATGACAATGATCTTCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTCTTCGAGGTACACTTGTATTCTTTTAGTGGAGCTTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATCATAAATGAATTTATAGATCATGCTGAGTTTGTCATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTCCAGAAACTTTTGGAGTGCTCGAGTCCAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAAAAGTTTCAACTATTGCAAACCATGTTGCAAGGTAACTATATATATTAATGATTTTCAATTTAAGCAAGGATCTATAACAAGTAAATTAAACTACGCTATGATTTGGTAAAGTTGATGTTAAATGGCTCAATTAGGGTGTTTTGTAAAAAAGTAACAATTTGGATAACGTTTTTTTTTTTTTTTATGGTACGCAGATCCAAATTCCAATGCATCGTTCTCTTATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGACATTTTCCTAACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATAAAGATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGCCGCTTCTCTTTCAAATGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCAATTTCAAACTCTAAACTTCATTTGGCAACTGTCACAGAGAATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACCACTGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGAAACCAACACATTGTGA

mRNA sequence

ATGGAAAGAACTATGCAAGTCATTCTCCTTCTAGCCCTAGTCTTTCTGTTTTCATCTCTCTCGACTTATTCCTTACCCTTGTCAACACGAGGAAGATGGATTATTGATTCGAAAACAGGACAACGAGTGAAACTCGTGTGTGTAAATTGGCCTTCTCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTGAAAGAACTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACGCACATGTTCACACGCTACGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGCTGTTGTTGATGTATTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTTGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACCACTATGTAACTCAAGGAGTAACCACGATCCATAACATAAATCCAGAAGTTTTAGTGATTGTTGGAGGCTTAAATTATGACAATGATCTTCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTCTTCGAGGTACACTTGTATTCTTTTAGTGGAGCTTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATCATAAATGAATTTATAGATCATGCTGAGTTTGTCATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTCCAGAAACTTTTGGAGTGCTCGAGTCCAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAAAAGTTTCAACTATTGCAAACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCTTATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGACATTTTCCTAACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATAAAGATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGCCGCTTCTCTTTCAAATGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCAATTTCAAACTCTAAACTTCATTTGGCAACTGTCACAGAGAATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACCACTGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGAAACCAACACATTGTGA

Coding sequence (CDS)

ATGGAAAGAACTATGCAAGTCATTCTCCTTCTAGCCCTAGTCTTTCTGTTTTCATCTCTCTCGACTTATTCCTTACCCTTGTCAACACGAGGAAGATGGATTATTGATTCGAAAACAGGACAACGAGTGAAACTCGTGTGTGTAAATTGGCCTTCTCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTGAAAGAACTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACGCACATGTTCACACGCTACGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGCTGTTGTTGATGTATTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAGCCAAGATGGTGTTGCTCTCTTGATGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTTGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACCACTATGTAACTCAAGGAGTAACCACGATCCATAACATAAATCCAGAAGTTTTAGTGATTGTTGGAGGCTTAAATTATGACAATGATCTTCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTCTTCGAGGTACACTTGTATTCTTTTAGTGGAGCTTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATCATAAATGAATTTATAGATCATGCTGAGTTTGTCATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTCCAGAAACTTTTGGAGTGCTCGAGTCCAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAAAAGTTTCAACTATTGCAAACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCTTATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGACATTTTCCTAACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATAAAGATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGCCGCTTCTCTTTCAAATGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCAATTTCAAACTCTAAACTTCATTTGGCAACTGTCACAGAGAATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACCACTGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGAAACCAACACATTGTGA

Protein sequence

MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL
Homology
BLAST of IVF0007865 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 7.1e-111
Identity = 221/541 (40.85%), Postives = 317/541 (58.60%), Query Frame = 0

Query: 9   LLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGLNHRPLK 68
           LL AL+ L  +  ++SLPL TRGRWI+D  TG RVKL CVNW  H +  L EGLN  P+ 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 69  ELADEAIKLRFNCVRLTYATHMFTR--YANRTVEENF-----------------DLLDLA 128
            +A     L FNCVRLTY+ HM TR  Y N TV + F                 +LLDL 
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 129 -------VVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQ 188
                  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+RYF+P  W++GL L+A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 189 RFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDNDLRC 248
            FNN   VV MSLRNE+RG       W+ ++  G  T+H  NP+VLVI+ GL +D DL  
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 249 LKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEGSN 308
           L   P+ +     K+V+E H YSF     +       N++C      F  +  FV   +N
Sbjct: 253 LPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSAN 312

Query: 309 --PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ---AE 368
               PLF+SE+G DQR V+D +NR+++C  A+LA++DLDWALWT  GSYYYR  +    +
Sbjct: 313 ATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKD 372

Query: 369 LPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPN-SNASFSYVIYHPQSGQCIEVSNDN 428
             ET+G    +W++I+NP+F+ + + +Q  +QDP  +   +  +IYHP SG C+E S   
Sbjct: 373 FEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGIG 432

Query: 429 KDIFLTNC-STSSRWSHD-NDSTPIKMSNTGLCLKASGEGLAASLSNDCLG-KQSVWSAI 488
             + L +C S  SRW++D +   PI +  +  C+   G GL A ++ +C     ++WS +
Sbjct: 433 NTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTV 492

Query: 489 SNSKLHLAT--VTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQ-DTQSQWFELVET 512
           S+++L L T  + ++GK   + ++ S S  I TN CIC TD     + + + QWF+++ T
Sbjct: 493 SSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDSHCYPKLNPEKQWFKVITT 547

BLAST of IVF0007865 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 5.6e-15
Identity = 80/360 (22.22%), Postives = 148/360 (41.11%), Query Frame = 0

Query: 29  TRGRWIIDSKTGQRVKLVCVNW-PSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYA 88
           T+G  I+D ++G+      +NW    T +  + GL  R + ++ D+  K  +N +RL Y+
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 89  THMF---TRYANRTVEENFDLLDLAVVDVL-------GASGLMVIADNHMSQPRWCCSLD 148
             +F   +R  +    +N DL+ L  + ++       G  G+ +I D H           
Sbjct: 101 NQLFDSSSRPDSIDYHKNPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHR---------- 160

Query: 149 DGNGFFGNRYFDPQ----EWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMME----NAN-D 208
            G+G     ++  Q     W+    ++A R+ N  TV+G  L NE  G       NA+ D
Sbjct: 161 PGSGGQSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTD 220

Query: 209 WNHYVTQGVTTIHNINPEVLVIVGGLNYD-----------NDLRCLKEKPLNVSTLDNKL 268
           W     +    I ++NP  L++V G++++            +L  +   P+ V  + N++
Sbjct: 221 WRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGVANYPV-VLDVPNRV 280

Query: 269 VFEVHLYSFSGASESKFVQQPL--NNICAKIINEFIDHAEFVIEGSNPFPLFVSEYGYDQ 328
           V+  H Y   G S   +   P   +N+ A       D     I   N  P+ V E+G   
Sbjct: 281 VYSPHDYG-PGVSSQPWFNDPAFPSNLPA-----IWDQTWGYISKQNIAPVLVGEFGGRN 340

Query: 329 REVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELPETFGVLESNWTQIKNP 356
            ++   E ++ +    ++   +L +  W+               +T G+L  +WT    P
Sbjct: 341 VDLSCPEGKWQNALVHYIGANNLYFTYWSL---------NPNSGDTGGLLLDDWTTWNRP 373

BLAST of IVF0007865 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 1.0e-13
Identity = 93/388 (23.97%), Postives = 153/388 (39.43%), Query Frame = 0

Query: 9   LLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVN-WPSHTQSMLIEGLNHRPL 68
           L  AL        +YS+      R I+D  +G+ V+L  VN +   T + ++ GL  R  
Sbjct: 12  LATALALAAGPAFSYSI---NNSRQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNW 71

Query: 69  KELADEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLAVVDVL-------GAS 128
           K++  +   L FN VRL +        T  A+     N DL  L  + +L        A 
Sbjct: 72  KDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQILDKVIAEFNAR 131

Query: 129 GLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRN 188
           G+ V+ D+H      C  + +   ++   Y + Q WL  L  VA R+ N   V+G+ L+N
Sbjct: 132 GMYVLLDHHTPD---CAGISE--LWYTGSYTEAQ-WLADLRFVANRYKNVPYVLGLDLKN 191

Query: 189 EIRGMM-----ENANDWNHYVTQGVTTIHNINPEVLVIV------------GGLNYDNDL 248
           E  G         A DWN    +G   +  + P+ L+ V            GG+ +  +L
Sbjct: 192 EPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDNPVCSTNGGIFWGGNL 251

Query: 249 RCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEG 308
           + L   PLN+    N+L+   H+Y      +S F      N    I            + 
Sbjct: 252 QPLACTPLNIPA--NRLLLAPHVYGPDVFVQSYFNDSNFPNNMPAIWERHFG------QF 311

Query: 309 SNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWAL-WTWQGSYYYREGQAELP 368
           +    L + E+G    E D  +  +      +L  K ++    W+W              
Sbjct: 312 AGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSW---------NPNSG 368

BLAST of IVF0007865 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.5e-278
Identity = 478/538 (88.85%), Postives = 495/538 (92.01%), Query Frame = 0

Query: 1   MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIE 60
           MERT+QVILLLALV +FSS S YSLPLST GRWIIDS++G+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL------------ 120
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL            
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 ------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180
                       AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNY 240
           LSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWN+YVTQGVTTIH INP VLVIV GLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEF 300
           DNDLRCLK+KPLNVSTLDNKL FEVHLYSFSG SESKFVQQPLNNICAKI++EFIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 ELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDN 420
           EL ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 KDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNS 480
           K+IFLTNCSTSSRWSHDNDSTPIKMS+TGLCLKASGEGL ASLS DC+GKQS+WSAISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLATVTENGKSLCLQ-IESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 514
            LHL TVTE+GKSLCLQ IESSNSSKIVTNSCICTT+DPTCLQDTQSQWFELV TNTL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of IVF0007865 vs. ExPASy TrEMBL
Match: A0A5D3D4P4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5066G00050 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.6e-267
Identity = 457/481 (95.01%), Postives = 457/481 (95.01%), Query Frame = 0

Query: 57  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL-------- 116
           MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL        
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLGQAKAGLT 60

Query: 117 ----------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 176
                           AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 120

Query: 177 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG 236
           WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG
Sbjct: 121 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG 180

Query: 237 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID 296
           GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID 240

Query: 297 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 356
           HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300

Query: 357 EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 416
           EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV
Sbjct: 301 EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 360

Query: 417 SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA 476
           SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA
Sbjct: 361 SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA 420

Query: 477 ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT 514
           ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT
Sbjct: 421 ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT 480

BLAST of IVF0007865 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.9e-261
Identity = 444/535 (82.99%), Postives = 477/535 (89.16%), Query Frame = 0

Query: 3   RTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGL 62
           RT   ILL+ALVF+FS LST SLPLSTRGRWIIDS+TG+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL-------------- 122
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL              
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ----------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 182
                     AVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDN 242
           LVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVTTIHNIN EVLVIV GLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVI 302
           DLRCLKEKPLNV TLDNKLVFEVHLYSFSG SESKFV+QPLN+ICA I+N FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 362
           +GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 PETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNKD 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QCI+VSNDNK+
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNSKL 482
           IFLT+CST +RWSH ND+TPIKMS+TGLCLKASGEGL ASLS DCL +QSVWSAISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 514
           HLATVT+ GKSLCLQI+SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of IVF0007865 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.3e-260
Identity = 446/537 (83.05%), Postives = 472/537 (87.90%), Query Frame = 0

Query: 1   MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIE 60
           M R  QV+  LAL F+F S   YSLPLST+ +WIIDS TG+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRIFQVVFFLAL-FVFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL------------ 120
           GLNHRPLKELADEAIKLRFNCVRLTYAT MFTRYANRTVEENFDLLDL            
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 121 ------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180
                       AVVDVLG SGLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNY 240
           LSLVAQRF+ KSTVVGMSLRNEIRG  ENANDWN+YVTQGVTTIHNINP VLVIV GLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEF 300
           DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSG  ESKF+ QPLNNICA IIN F+DHAEF
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360
           V EGSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 ELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDN 420
           E  ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI+VSNDN
Sbjct: 361 EPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 KDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNS 480
           KDIF+ NCS SSRW+HDNDSTPI+MS+ GLCLK  GEGL  SLS DCLG QS WSAISN+
Sbjct: 421 KDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNT 480

Query: 481 KLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 514
           KLHLAT++++GKSLCLQ+ESSNSSKIVTNSCICT   P CLQDT+SQWFELV+TNTL
Sbjct: 481 KLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

BLAST of IVF0007865 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.3e-260
Identity = 442/535 (82.62%), Postives = 475/535 (88.79%), Query Frame = 0

Query: 3   RTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGL 62
           RT   ILLLALVF+FS LST SLPLSTRGRWIID +TG+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL-------------- 122
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL              
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ----------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 182
                     AVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDN 242
           LVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVTTIHNIN EVLVIV GLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVI 302
           DLRCLKEKPLNV TLDNKLVFEVHLYSFSG SESKFV+QPLNNICA I+N FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 362
           +GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 PETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNKD 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSNASFSYVIYHPQ+ QCI+VSNDNK+
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 423 IFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNSKL 482
           IFLT+CST +RWSH ND+TPIKMS+TGLCLKASGEGL ASLS DCL +QSVWSAISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 483 HLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 514
           HLATV + G SLCLQI+SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 482 HLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of IVF0007865 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 478/538 (88.85%), Postives = 495/538 (92.01%), Query Frame = 0

Query: 1   MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIE 60
           MERT+QVILLLALV +FSS S YSLPLST GRWIIDS++G+RVKLVCVNWPSHTQSMLIE
Sbjct: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL------------ 120
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL            
Sbjct: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148

Query: 121 ------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180
                       AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG
Sbjct: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208

Query: 181 LSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNY 240
           LSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWN+YVTQGVTTIH INP VLVIV GLNY
Sbjct: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268

Query: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEF 300
           DNDLRCLK+KPLNVSTLDNKL FEVHLYSFSG SESKFVQQPLNNICAKI++EFIDHAEF
Sbjct: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328

Query: 301 VIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388

Query: 361 ELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDN 420
           EL ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSNDN
Sbjct: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448

Query: 421 KDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNS 480
           K+IFLTNCSTSSRWSHDNDSTPIKMS+TGLCLKASGEGL ASLS DC+GKQS+WSAISNS
Sbjct: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508

Query: 481 KLHLATVTENGKSLCLQI-ESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 513
            LHL TVTE+GKSLCLQI ESSNSSKIVTNSCICTT+DPTCLQDTQSQWFELV TNTL
Sbjct: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 566

BLAST of IVF0007865 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 478/538 (88.85%), Postives = 495/538 (92.01%), Query Frame = 0

Query: 1   MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIE 60
           MERT+QVILLLALV +FSS S YSLPLST GRWIIDS++G+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL------------ 120
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL            
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 ------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180
                       AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNY 240
           LSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWN+YVTQGVTTIH INP VLVIV GLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEF 300
           DNDLRCLK+KPLNVSTLDNKL FEVHLYSFSG SESKFVQQPLNNICAKI++EFIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360
           VIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 ELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDN 420
           EL ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 KDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNS 480
           K+IFLTNCSTSSRWSHDNDSTPIKMS+TGLCLKASGEGL ASLS DC+GKQS+WSAISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLATVTENGKSLCLQI-ESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 513
            LHL TVTE+GKSLCLQI ESSNSSKIVTNSCICTT+DPTCLQDTQSQWFELV TNTL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of IVF0007865 vs. NCBI nr
Match: KAA0045387.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK18506.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 457/481 (95.01%), Postives = 457/481 (95.01%), Query Frame = 0

Query: 57  MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL-------- 116
           MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL        
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLGQAKAGLT 60

Query: 117 ----------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 176
                           AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 120

Query: 177 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG 236
           WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG
Sbjct: 121 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG 180

Query: 237 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID 296
           GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID 240

Query: 297 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 356
           HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300

Query: 357 EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 416
           EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV
Sbjct: 301 EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 360

Query: 417 SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA 476
           SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA
Sbjct: 361 SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA 420

Query: 477 ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT 513
           ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT
Sbjct: 421 ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT 480

BLAST of IVF0007865 vs. NCBI nr
Match: XP_023533776.1 (uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 452/537 (84.17%), Postives = 475/537 (88.45%), Query Frame = 0

Query: 1   MERTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIE 60
           M RT QV+L LAL F+F S S YSLPLST+GRWIIDS TG+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRTFQVVLFLAL-FVFLSPSVYSLPLSTKGRWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL------------ 120
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL            
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEPAKAGLAQNNP 120

Query: 121 ------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180
                       AVVDVLG SGLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNY 240
           LSLVAQRF+ KSTVVGMSLRNEIRG  ENANDWN+YVTQGVTTIHNINP VLVIV GLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEF 300
           DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSG SESKF+ QPLNNICA IIN F+DHAEF
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360
           V EGSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 ELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDN 420
           E  E FGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI+VSNDN
Sbjct: 361 EPGEAFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 KDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNS 480
           KDIFL NCS SSRW+HDNDSTPI+MS+TGLCLK SGEGL  SLS DCLG Q+ WSAISN+
Sbjct: 421 KDIFLGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCLGPQNSWSAISNT 480

Query: 481 KLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 513
           KLHLAT+  + KSLCLQIESSNSSKIVTNS ICT   P CL+DT+SQWFELV+TNTL
Sbjct: 481 KLHLATIAPDEKSLCLQIESSNSSKIVTNSFICTNGAPNCLEDTRSQWFELVKTNTL 536

BLAST of IVF0007865 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 444/535 (82.99%), Postives = 477/535 (89.16%), Query Frame = 0

Query: 3   RTMQVILLLALVFLFSSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGL 62
           RT   ILL+ALVF+FS LST SLPLSTRGRWIIDS+TG+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL-------------- 122
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL              
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ----------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 182
                     AVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDN 242
           LVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVTTIHNIN EVLVIV GLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVI 302
           DLRCLKEKPLNV TLDNKLVFEVHLYSFSG SESKFV+QPLN+ICA I+N FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 362
           +GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 PETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNKD 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QCI+VSNDNK+
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSAISNSKL 482
           IFLT+CST +RWSH ND+TPIKMS+TGLCLKASGEGL ASLS DCL +QSVWSAISNSKL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNTL 513
           HLATVT+ GKSLCLQI+SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of IVF0007865 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 392.1 bits (1006), Expect = 6.8e-109
Identity = 203/521 (38.96%), Postives = 313/521 (60.08%), Query Frame = 0

Query: 18  SSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKL 77
           +++   S PLST  RWI+D + G RVKLVC NWPSH Q ++ EGL+ +P+  +A + +++
Sbjct: 26  NTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEM 85

Query: 78  RFNCVRLTYATHMFTRYA---NRTVEENFDLLDL------------------------AV 137
            FNCVRLT+   + T      N TV ++F  L L                         V
Sbjct: 86  GFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTV 145

Query: 138 VDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTV 197
           V  LG + +MVI DNH+++P WCC+ DDGNGFFG+++FDP  W+  L  +A  FN  S V
Sbjct: 146 VTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNV 205

Query: 198 VGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDNDLRCLKEKPLNV 257
           VGMSLRNE+RG  +N NDW  Y+ QG   +H+ N +VLVI+ GL++D DL  ++ +P+ +
Sbjct: 206 VGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKL 265

Query: 258 STLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEGSNPFPLFVSE 317
           S    KLVFE+H YSFS    + +     N+IC +++N   +   +++  +  FPLF+SE
Sbjct: 266 S-FTGKLVFELHWYSFSDG--NSWAANNPNDICGRVLNRIGNGGGYLL--NQGFPLFLSE 325

Query: 318 YGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELPETFGVLESNWT 377
           +G D+R V+  +NR+  C T   A+ D+DW+LW   GSYY R+G+  + E +GVL+S+W 
Sbjct: 326 FGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWI 385

Query: 378 QIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVS-NDNKDIFLTNCSTSSR 437
            ++N +F+QK   LQ+ LQ P        +++HP +G CI  S +D K + L  C++S  
Sbjct: 386 SVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEP 445

Query: 438 WSHDNDSTPIKMSNTGLCLKASGEGLAASLS-NDCLGKQSVWSAISNSKLHLATVTENGK 497
           WS+   +  IK  +  LCL+++G     +++   C    S W  IS S++HLA+ T N  
Sbjct: 446 WSYTKKALRIK--DQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKT 505

Query: 498 SLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVE 510
           SLCL ++++N+  +V N+C C + D +C  +  SQWF++++
Sbjct: 506 SLCLDVDTANN--VVANACKCLSKDKSC--EPMSQWFKIIK 534

BLAST of IVF0007865 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.8e-100
Identity = 214/543 (39.41%), Postives = 306/543 (56.35%), Query Frame = 0

Query: 5   MQVILLLALVFLFSSLSTYSLPLSTRGRWII-DSKTGQRVKLVCVNWPSHTQSMLIEGLN 64
           M+    +++  L   ++T++ P ST  RWI+ D   G+RVKL CVNWPSH ++ + EGL+
Sbjct: 1   MEKFFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLS 60

Query: 65  HRPLKELADEAIKLRFNCVRLTYATHMFTR---YANRTVEE-----------------NF 124
            +PL  +A++ + + FNCVRLT+  ++ T     A  TV +                 N 
Sbjct: 61  KQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNP 120

Query: 125 DLLDL-------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 184
            +LDL        VV  L    +MVI DNH+SQP WCCS +DGNGFFG+++ +PQ W++G
Sbjct: 121 TILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKG 180

Query: 185 LSLVAQRFNN-KSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLN 244
           L  +A  F N  S VVGMSLRNE+RG  +N  DW  Y+ +G   +H++NP VLVIV GLN
Sbjct: 181 LKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLN 240

Query: 245 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAE 304
           Y  DL  L+E+P  VS    K+VFE+H Y F    E       LN IC K   + +  + 
Sbjct: 241 YATDLSFLRERPFEVS-FRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSG 300

Query: 305 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 364
           F++E     PLFVSE+G DQR  +  +N+F+SCF A  A +DLDW+LWT  GSYY RE  
Sbjct: 301 FLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKS 360

Query: 365 AELPETFGVLESNWTQIKNPNFVQKFQLLQT-----MLQDPNSNASFSYVIYHPQSGQCI 424
               E++GVL+ NW+ I+N   +Q    +QT     M   P        +++HP +G CI
Sbjct: 361 IGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPSTGLCI 420

Query: 425 EVSNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASL-----SNDCLG 484
            V      + L +C+ S  W   +           LCLKA  +G +  L      + C  
Sbjct: 421 -VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYC-- 480

Query: 485 KQSVWSAISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWF 509
             S W   S+SK+ L+++T+NG S+CL +++ N++ IVTNSC C   + +C  D +SQWF
Sbjct: 481 --SKWKLFSDSKMQLSSITKNGFSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQWF 522

BLAST of IVF0007865 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.4e-98
Identity = 193/517 (37.33%), Postives = 298/517 (57.64%), Query Frame = 0

Query: 26  PLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLT 85
           PLST  RWIID K GQRVKL CVNWPSH Q ++ EGL+ + + +LA + + + FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRYA---NRTVEENFDLLDL------------------------AVVDVLGASG 145
           +   + T      N TV ++F  L L                         VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 205
           +MVI DNH+++P WCC  +DGNGFFG+ +FDP  W+ GL+ +A  F   + VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 IRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDNDLRCLKEKPLNVSTLDNKLV 265
           +RG  +N +DW  Y+ QG   +H  NP VLVI+ GL+YD DL  ++ + +N+ T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 266 FEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEGSNPFPLFVSEYGYDQREV 325
           FE+H YSF+  + + +  +  N  C +I+    +   F +     FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGK 303

Query: 326 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELPETFGVLESNWTQIKNPNFV 385
           +  +NR++ C     A+ D+DW++WT QGSYY REG   + E +G+L+S+W ++++ +F+
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 386 QKFQLLQTMLQDPNSNASFSYVIYHPQSGQC-IEVSNDNKDIFLTNCSTSSRWSHDNDST 445
           Q+  L+ + LQ P S +    +++HP +G C ++   D   + L  C+ S  WS+   +T
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 446 PIKMSNTGLCLKASGEGLAASLSNDCLGKQ--SVWSAISNSKLHLATVTENGKSLCLQIE 505
            + + +  LCL+++G      LS         S W  IS S + LA  + N  SLCL ++
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTN-NSLCLDVD 483

Query: 506 SSNSSKIVTNSCICTT-DDPTCLQDTQSQWFELVETN 512
            +N+  ++ ++C C   +D +C  D  SQWF++V+ +
Sbjct: 484 ETNN--LMASNCKCVKGEDSSC--DPISQWFKIVKVS 507

BLAST of IVF0007865 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-91
Identity = 193/536 (36.01%), Postives = 295/536 (55.04%), Query Frame = 0

Query: 6   QVILLLALVFLFSSLSTYSL----PLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEG 65
           + I L   +FLF SL + +L    PL T+ RWI+++K G RVKL C NWPSH + ++ EG
Sbjct: 3   KAIALTVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEG 62

Query: 66  LNHRPLKELADEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDL---------- 125
           L+ +P+  ++ +   + FNCVRLT+   +    T   N TV+++F+   L          
Sbjct: 63  LSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTH 122

Query: 126 --------------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWL 185
                         AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+
Sbjct: 123 NPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWM 182

Query: 186 QGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGL 245
            GL  +A  F N   VVGMSLRNE+RG    + DW  Y+ +G   +H  NP VLVI+ GL
Sbjct: 183 LGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGL 242

Query: 246 NYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHA 305
           N+D DL  LK++P+N+S    KLV E+H YSF+  +  ++    +N+ C+++ ++     
Sbjct: 243 NFDADLSFLKDRPVNLS-FKKKLVLELHWYSFTDGT-GQWKSHNVNDFCSQMFSKERRTG 302

Query: 306 EFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREG 365
            FV++    FPLF+SE+G DQR  D   NR+M+C  A  A+KDLDWA+W   G YY+REG
Sbjct: 303 GFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREG 362

Query: 366 QAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSN 425
           +  + E +G+L++NW  + N  ++++  ++Q     P    +    I+HP +G C+   +
Sbjct: 363 KRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKS 422

Query: 426 --DNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKA-SGEGLAASLSNDCLGKQSVWS 485
                ++ L  C+    WS+ +            CL+  +  G +  L   C   +    
Sbjct: 423 HCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ--- 482

Query: 486 AISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFEL 508
            IS +K+HL+  T +G  +CL ++S N+  +V NSC C T D TC  +  SQWF++
Sbjct: 483 -ISATKMHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of IVF0007865 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.9e-80
Identity = 190/531 (35.78%), Postives = 278/531 (52.35%), Query Frame = 0

Query: 13  LVFLF---SSLSTYSLPLSTRGRWIIDSKTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKE 72
           L FLF   +S  T S PLST+ RWI+D K GQRVKL CVNWP+H Q  + EGL+ +PL  
Sbjct: 9   LFFLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAEGLSKQPLDS 68

Query: 73  LADEAIKLRFNCVRLTYATHMFTR---YANRTVEENFDLLDL------------------ 132
           ++ + + + FNCVRLT+   + T        TV+++F+ L L                  
Sbjct: 69  ISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLP 128

Query: 133 ------AVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQ 192
                  VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GL  +A 
Sbjct: 129 LFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMAT 188

Query: 193 RFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVGGLNYDNDLRC 252
            F N + V+GMSLRNE RG  +  + W  ++ QG   +H  NP++LVI+ G+++D +L  
Sbjct: 189 LFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSF 248

Query: 253 LKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFIDHAEFVIEGSN 312
           L+++ +NVS  D KLVFE+H YSFS   +S + +   N+ C KII +   +  F++    
Sbjct: 249 LRDRSVNVSFTD-KLVFELHWYSFSDGRDS-WRKHNSNDFCVKIIEKVTHNGGFLL--GR 308

Query: 313 PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELPETF 372
            FPL +SE+G DQR  D + NR+M+C  A  A+ DLDWA+W   G YY R G        
Sbjct: 309 GFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPG------ 368

Query: 373 GVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI--EVSNDNKDIF 432
                                       PN N     +++HP +G C+    S++   + 
Sbjct: 369 --------------------------LRPNKN-----LLFHPSTGLCVTNNPSDNIPTLR 428

Query: 433 LTNCSTSSRWSHDNDSTPIKMSNTGLCLKAS---GEGLAASLSNDCLGKQSVWSAISNSK 492
           L  C  S  W+  N S  I   N  +C++A    G+ +   +   C    S    IS +K
Sbjct: 429 LGPCPKSDPWTF-NPSEGILWINK-MCVEAPNVVGQKVKLGVGTKC----SKLGQISATK 488

Query: 493 LHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELV 509
           +HL+  T NG  LCL ++  ++S +V N C   T D +C  D  SQWF+++
Sbjct: 489 MHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA07.1e-11140.85Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P235485.6e-1522.22Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
P194871.0e-1323.97Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
A0A0A0K8534.5e-27888.85Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
A0A5D3D4P43.6e-26795.01Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7TMC93.9e-26182.99Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1JZU84.3e-26083.05Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=... [more]
A0A5A7THG17.3e-26082.62Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
KAE8646606.10.088.85hypothetical protein Csa_005432 [Cucumis sativus][more]
XP_011658389.10.088.85glycosyl hydrolase 5 family protein [Cucumis sativus][more]
KAA0045387.10.095.01major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK18506.1 ma... [more]
XP_023533776.10.084.17uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo][more]
KAA0042711.10.082.99major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G13130.16.8e-10938.96Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.15.8e-10039.41Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.12.4e-9837.33Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.12.9e-9136.01Glycosyl hydrolase superfamily protein [more]
AT5G16700.13.9e-8035.78Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 63..326
e-value: 1.5E-26
score: 93.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..375
e-value: 7.2E-65
score: 221.5
NoneNo IPR availableGENE3D2.80.10.50coord: 380..509
e-value: 2.8E-5
score: 26.3
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 7..108
coord: 109..511
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 376..507
score: 8.76
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..362
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 376..488

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0007865.1IVF0007865.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds