IVF0006446 (gene) Melon (IVF77) v1

Overview
NameIVF0006446
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPectinesterase
Locationchr05: 7882207 .. 7885511 (+)
RNA-Seq ExpressionIVF0006446
SyntenyIVF0006446
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGACTATTATTCGTGGGATTTTTGTTTTGATGATGATAATATTTGTAAGAGGAAATGAAGGTGAATCTTATAGAAGGATAGGAAGGAAGAAATTTGCATGGAAGACTTTAATTGTTGACAAGAATGGCCATGGAAACTTCTCTACTATTCAAGCTGCCATTGATTCTGTTCCTTCTGACAATAGATTTTGGGTCTCCATCCATATTCGACCTGGTCTTTACAGGTATTTATTATTATATAATTTTGAATTCTTCTTTCCCTAAAGAACCCCTTAATTCGATAGTTGTTAGAAATAGCTACGCATTGTGACAAAAAATATATTAAATAGGGTCTGAGTTTAGTTATTGGGGTTTAGGTTTTTGTTAGGGCAACACTAGTTTGATATTTTGAGGGGTTTGTTATTATTATTATTATTTTGAGTTGATGGGTTGGTTCATCTTCAATTAAGTTGGTAGAGCAAGTGATTTGATTTTCAGTGTAATTATTTTTTAATAACTAGATTATAATATGTCAATTAGGTTTCAATTTTTAAGTAAAAAAGATTGAGGCTATTGAATAATTTTTTAAAACAGAGATTATAAATACTGATACGCTCCTTTCTTTGTTTTGTTATATGATCATCTTACTTTTGGAACTATTTTCAAAATCTAAGCTACAGTATTGAAAACTGAGGGTGTTTTGGCTTTGTAATTTAGTTTATTTCAAATTCAAATGTTTGAAAATCAAATCTGTTTTAAGAAATCCAGTGCAATTTCCAAATATATAAATTATGATAATTAACTATTTGGTTTCTAAAATTTGTTTTTCTCTTGCAAGTTTTTGGAAATTTTTTAAAATAAAAAGTAAAAAATCTAATGCTTACTAAATAGTCAATTGAACAGTGATTATATTTGCATCTCATTTCGATTGTTTCCTAAAATAATAACTTTTAGTTTCCGAAATTAACGTACATTAAATTTTATATTATATATACTATAAGATGTAGAGATTATTACTGAATTTAAAAAGTTTGTAGACAAAAAGTGATCGGTTGAGAATTTAAAAAGTGATTATATTATTGAACAATAGTTAGAAAATGGGACGTTGATTTTGCTATATTTCCCTTATTTGTTTTTGTATACAGAGAGAAGGTGAAGATTCCATATGATAAACCATATATAATTTTGAAAGGACATAGAAAAAGAAACACAAAGGTTGTTTGGGATGATCATCTCACTGTTGCTCAAAGCCCTACTTTCACCTCTTCAGCTGATAACATTGTCGTCAAATCCATCTCTTTTGTGGTGAGTAAAACTTTTAATTATATATATATAAAATTCAAAAGATAAAGAAAACTTGATTATACAAATCGTATGTGTGATACCAAATTGTTCGTTGTTAGAATTCTTATAATTATCCATGGAAAAATGGAAATCCAAGAGTTCCAGCAGTGGCAGCAATGATCACAGGGGACAAGTCGTCTTTCTATAGATGTGGTTTCTATGGAGTTCAAGACACATTGTGGGATAATCAAGGACGACATTACTATCATCGTTGCACCATTCAAGGAGCTGTCGACTTTATTTTTGGAGCTGCCCAGTCCATTTTTCAGGTTTTTTTTCTTCTCATCCTTACAGATTTGAGTCCTACTTCTTAGTTATTTAGTATTTGACAAAATACTAAAATGACGTTAGTAAAAATTCAAGGCTAAATTTGTAAAATATTGAAACATAGATACTAAATTGAAAATAATTGTAACCTTTTGAAATTTATGGAGATTTAAAGGTTATTTTTTCTTTATTCAGTAATAAATACAAAGTCAAATGATTTGATAGACAAGGTCATAAGTAGCTATTCACTTGATTTTATCAAGTCTTGCTTTCATTTTAATTAACCATTGAAGTTGTTAAGTTAGGTTGGACTCCATTATCGAAAAATAGTTTTGATGTTGATCATTTTTGTTATAACCTTTCTATATCTAGTCTAATTTTACACCTTTCAATATAATAGACCTCTTAAATGATTTAACTTCACATATTTTGTTTAAAATAGAGTCGTTAAATTGTTCCATATTTCAATATGCATAATAACGAAATGAAAAGAGAAGACTTACAAAACAAAACAAAGTATTTAAGCTAAAGGACTATGCCAATGAATACTCAATAATTTAATTAGTAAGATTCACAGCTAATGTTTTATTATAAATGTAAAAATGTTGGGGGTGATATATACTATCAGGGATGTTCAATATCAGTGGTTGGAGAAGCACTGCTTCCATTAGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGATCCAAATGATGCAAATGGTTTTGTATTCAAAGAATGCAATGTGTTTGGGAGTGGGTCAGCATACTTAGGAAGGCCATGGAGGGCTTATTCAAGAGTTATCTTTCACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCATTTTGTTGGTTATGAGTAAGATTCATACATCCTTTATTTTTATTATTCATTCGTTTATAAATCTCCTTTCCACTTTCTTCTTCTATCAAACAAAAAATATTGTCCTTTAAATGCATTTTGTTGTAATAGACTTCAAATAAGATGTGACATGATGGGGGGGGTGTATTTATGTTTTAATTTATACTTGAATACTATTATTTTTGAATACTTTAGGCTATTTTATGATATGTTGTTTCATTATTTTAAGGTGGGTACGTATACGAAAAGGATTGATTGGGATTTTGGGGTTGGAAATGTGGTTTTAAGAGGTTTATTAAGAAACGGGCCTCTTTGGTTTCTTTCTTGGCTTTTGGAGTTAGGATTCCAAATTTCAATCATGGTTTTTTTTCCTTTTTTCTTCTTCTTCTTTTGGCTAATCCTTACTATAACAATGAATATAATTATATTGTTGACTCATACAATAATAAATTTTGGCTTATCCTAATATCTATCATCTATCTTTTAAAAGTTTAGGGTTTAGTCAATTTATAAATTCTAAATTTCAAATAAAATAAATAAATATTTTACATGTACTAAAAAAAATTTTAGAAATATTTATTAGAAAGAGAAGGAATAGATGTCGTTGAATCTAAGTTGCTTAGGTTTTTTTCTTTTTATACTAAATATTTTTATTTTCAAAATTCAGGAACCAATTGACTTACGCAGAAAATGATTGTTACGGACCTGGTTCTGACATATCACGTCGTGTCTCGTGGGAAAAGAAGCTAAGTCCGAAAGACATACGACAATTAACTAGCATGAGTTTTATCGACGTTGAAGGTTGGATCCAAGACCAACCTTGGTTATAA

mRNA sequence

ATGTGGACTATTATTCGTGGGATTTTTGTTTTGATGATGATAATATTTGTAAGAGGAAATGAAGGTGAATCTTATAGAAGGATAGGAAGGAAGAAATTTGCATGGAAGACTTTAATTGTTGACAAGAATGGCCATGGAAACTTCTCTACTATTCAAGCTGCCATTGATTCTGTTCCTTCTGACAATAGATTTTGGGTCTCCATCCATATTCGACCTGGTCTTTACAGAGAGAAGGTGAAGATTCCATATGATAAACCATATATAATTTTGAAAGGACATAGAAAAAGAAACACAAAGGTTGTTTGGGATGATCATCTCACTGTTGCTCAAAGCCCTACTTTCACCTCTTCAGCTGATAACATTGTCGTCAAATCCATCTCTTTTGTGAATTCTTATAATTATCCATGGAAAAATGGAAATCCAAGAGTTCCAGCAGTGGCAGCAATGATCACAGGGGACAAGTCGTCTTTCTATAGATGTGGTTTCTATGGAGTTCAAGACACATTGTGGGATAATCAAGGACGACATTACTATCATCGTTGCACCATTCAAGGAGCTGTCGACTTTATTTTTGGAGCTGCCCAGTCCATTTTTCAGGGATGTTCAATATCAGTGGTTGGAGAAGCACTGCTTCCATTAGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGATCCAAATGATGCAAATGGTTTTGTATTCAAAGAATGCAATGTGTTTGGGAGTGGGTCAGCATACTTAGGAAGGCCATGGAGGGCTTATTCAAGAGTTATCTTTCACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCATTTTGTTGGTTATGAGAACCAATTGACTTACGCAGAAAATGATTGTTACGGACCTGGTTCTGACATATCACGTCGTGTCTCGTGGGAAAAGAAGCTAAGTCCGAAAGACATACGACAATTAACTAGCATGAGTTTTATCGACGTTGAAGGTTGGATCCAAGACCAACCTTGGTTATAA

Coding sequence (CDS)

ATGTGGACTATTATTCGTGGGATTTTTGTTTTGATGATGATAATATTTGTAAGAGGAAATGAAGGTGAATCTTATAGAAGGATAGGAAGGAAGAAATTTGCATGGAAGACTTTAATTGTTGACAAGAATGGCCATGGAAACTTCTCTACTATTCAAGCTGCCATTGATTCTGTTCCTTCTGACAATAGATTTTGGGTCTCCATCCATATTCGACCTGGTCTTTACAGAGAGAAGGTGAAGATTCCATATGATAAACCATATATAATTTTGAAAGGACATAGAAAAAGAAACACAAAGGTTGTTTGGGATGATCATCTCACTGTTGCTCAAAGCCCTACTTTCACCTCTTCAGCTGATAACATTGTCGTCAAATCCATCTCTTTTGTGAATTCTTATAATTATCCATGGAAAAATGGAAATCCAAGAGTTCCAGCAGTGGCAGCAATGATCACAGGGGACAAGTCGTCTTTCTATAGATGTGGTTTCTATGGAGTTCAAGACACATTGTGGGATAATCAAGGACGACATTACTATCATCGTTGCACCATTCAAGGAGCTGTCGACTTTATTTTTGGAGCTGCCCAGTCCATTTTTCAGGGATGTTCAATATCAGTGGTTGGAGAAGCACTGCTTCCATTAGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACAGATCCAAATGATGCAAATGGTTTTGTATTCAAAGAATGCAATGTGTTTGGGAGTGGGTCAGCATACTTAGGAAGGCCATGGAGGGCTTATTCAAGAGTTATCTTTCACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCATTTTGTTGGTTATGAGAACCAATTGACTTACGCAGAAAATGATTGTTACGGACCTGGTTCTGACATATCACGTCGTGTCTCGTGGGAAAAGAAGCTAAGTCCGAAAGACATACGACAATTAACTAGCATGAGTTTTATCGACGTTGAAGGTTGGATCCAAGACCAACCTTGGTTATAA

Protein sequence

MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL
Homology
BLAST of IVF0006446 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 7.6e-114
Identity = 188/300 (62.67%), Postives = 235/300 (78.33%), Query Frame = 0

Query: 38  LIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 97
           + VD++GHGNF+TIQ AIDSVP +NR W  I+++ GLYREK+KIPY+KP+I+L G  KR 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 98  TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWK---NGNPRVPAVAAMITGDK 157
           T+V WDDH +VAQSPTF++ ADN VVKSI+F NSYN+P K   N NPR PAVAA+I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 158 SSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLG 217
           S+FY  GF G+QDTLWD  GRHY+HRCTIQGAVDFIFG  QSI+Q C I V+G  L P G
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEP-G 213

Query: 218 STSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGW 277
              +ITAQGRT+P DANGF+F  C V+G+G A+LGRPWR YSRVIF+NSN ++++ P GW
Sbjct: 214 LAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 278 DPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
           D W+FVG+ENQL +AE+ C+G G++I RRV W KKLS   I+ L  +SFI+  GW++D P
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 332

BLAST of IVF0006446 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-108
Identity = 182/300 (60.67%), Postives = 227/300 (75.67%), Query Frame = 0

Query: 38  LIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 97
           +IVD++GHGNF+TIQ AIDSVP +N  W  I+++ GLYREK+ IP  KP+I++ G  KR+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWK---NGNPRVPAVAAMITGDK 157
           T+V WDDH ++AQSPTF + ADN VVK I+F NSYN+P     N NPRVPAVAA I GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 158 SSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLG 217
           S+FY  GF G+QDTLWD+ GRHY+HRCTIQGAVDFI G+ QSI+Q C I V+G  L P G
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP-G 208

Query: 218 STSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGW 277
            T +ITAQGRT+ NDANGFVF  C V G G AYLGR WR YSRVIF+NSN +++++P GW
Sbjct: 209 VTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGW 268

Query: 278 DPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
             W++ GYE QLTYAE+ C+G GS+ SRR  W KKLS   ++ L  +SFI+  GW++D P
Sbjct: 269 WEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVEDLP 327

BLAST of IVF0006446 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 145/299 (48.49%), Postives = 185/299 (61.87%), Query Frame = 0

Query: 36  KTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRK 95
           KT+IV+ N    F T+Q+AIDS+P  N+ W+ I I  G+Y EKV IP  K YI ++G   
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 96  RNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKS 155
             T + + DH     S TFTS   NI++  I+F N YN    + +P  PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 156 SFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGS 215
           +     F G QDTL+D+ GRHYY RC I G +DFIFG AQSIF+GC++ +      P   
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 216 TSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWD 275
              ITAQGR  P D  GFVFK+C V GSG A LGR W++YSRVIF+ S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 276 PWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
            W   G E  +T+ E  C G G+D S+RV W  K S KD+ Q T+++FID EGW+   P
Sbjct: 279 AWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWLSRLP 336

BLAST of IVF0006446 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 278.9 bits (712), Expect = 7.7e-74
Identity = 140/327 (42.81%), Postives = 194/327 (59.33%), Query Frame = 0

Query: 8   IFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVS 67
           + +++M +FV G    +      ++ A+ T+ VD NG GNF+T+Q+AIDS+   N  W+ 
Sbjct: 12  VCMVVMTLFVYGYSAAA------EQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIR 71

Query: 68  IHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSIS 127
           +  + G+YREKV IP +K +I L+G     T + +DDH     S TFT+ AD+IV+  I+
Sbjct: 72  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 131

Query: 128 FVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAV 187
           F N+YN    N    VPAVAA + GD+       F G+QDTL+D +GRHYY RC I G +
Sbjct: 132 FKNTYNIVPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGI 191

Query: 188 DFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAY 247
           DFIFG  QS+F+ C++++      P      ITA  R  P+D  GFVF +C V G G   
Sbjct: 192 DFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTL 251

Query: 248 LGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDISRRVSWE 307
           LGR W + +RVIF  S  S+++ P GWD W   G E  LT+ E  C G G+D S+RV W 
Sbjct: 252 LGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWL 311

Query: 308 KKLSPKDIRQLTSMSFIDVEGWIQDQP 335
           KKLS  ++    S+SFID +GWI   P
Sbjct: 312 KKLSLSEVDGFASVSFIDQDGWISRFP 331

BLAST of IVF0006446 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.1e-63
Identity = 123/297 (41.41%), Postives = 174/297 (58.59%), Query Frame = 0

Query: 40  VDKNGHGNFSTIQAAIDSVPS--DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 99
           VD++G G+FS IQ AI+S+P   +N     I ++PG+YREKV IP +KPYI L G +  N
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 100 TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSF 159
           T ++W D   + +SPT T  A + V + ++  N +    +       AVA  +  DK++F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGR-------AVALRVAADKAAF 172

Query: 160 YRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTS 219
           Y C     QDTL D+ G HY+  C I+GA DFI G+A S+++ C +     +L P  +  
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSP--NNG 232

Query: 220 FITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPW 279
            ITAQ RT   + +GF F  C + GSGS +LGRPW AYSRV+F  S FSN++ P GW+ W
Sbjct: 233 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 292

Query: 280 HFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
                EN + Y E  CYGPG+D  +RV W K+LS ++     S  FI  + W++  P
Sbjct: 293 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAP 336

BLAST of IVF0006446 vs. ExPASy TrEMBL
Match: A0A1S3C544 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 5.3e-203
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60
           MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS
Sbjct: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60

Query: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120
           DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN
Sbjct: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120

Query: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180
           IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR
Sbjct: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180

Query: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240
           CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV
Sbjct: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240

Query: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI 300
           FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI
Sbjct: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI 300

Query: 301 SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL 337
           SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL
Sbjct: 301 SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL 336

BLAST of IVF0006446 vs. ExPASy TrEMBL
Match: A0A5D3CTI5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.1e-168
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60
           MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS
Sbjct: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60

Query: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120
           DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN
Sbjct: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120

Query: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180
           IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR
Sbjct: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180

Query: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240
           CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV
Sbjct: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240

Query: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 284
           FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE
Sbjct: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 283

BLAST of IVF0006446 vs. ExPASy TrEMBL
Match: A0A6J1KUQ1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.6e-167
Identity = 269/324 (83.02%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 12  MMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIR 71
           MMIIFVRGNE E YR +G KK AW+T++VD++GHGNFSTIQAAIDSVPS+N FWVSIHI 
Sbjct: 3   MMIIFVRGNENERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWVSIHIN 62

Query: 72  PGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNS 131
           PG+YREKVKIPYDKPYIILKG R++ TKVVWDDH T+AQSPTFTSSADN+VVKSI+FVNS
Sbjct: 63  PGIYREKVKIPYDKPYIILKGQRRKTTKVVWDDHFTLAQSPTFTSSADNVVVKSITFVNS 122

Query: 132 YNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIF 191
           YN+PW NGN R PAVAAMITGDK+SFY+CGFYGVQDTLWD+QGRHYYHRC IQGAVDFIF
Sbjct: 123 YNFPWTNGNRRAPAVAAMITGDKASFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIF 182

Query: 192 GAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRP 251
           G+AQSIFQGC ISVVGEA LPLGSTSFITAQGRTDPNDANGFVFK+C VFGSGSA+LGRP
Sbjct: 183 GSAQSIFQGCLISVVGEAFLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 242

Query: 252 WRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLS 311
           WR YSRVIF+NS+FSNIINPNGWDPWHFVG+E+QLTYAE  CYGPGSD+S RV WEKKL 
Sbjct: 243 WRGYSRVIFYNSSFSNIINPNGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKKLD 302

Query: 312 PKDIRQLTSMSFIDVEGWIQDQPW 336
             +I+QLT MSFID EGW+QDQPW
Sbjct: 303 LNEIQQLTDMSFIDTEGWMQDQPW 326

BLAST of IVF0006446 vs. ExPASy TrEMBL
Match: A0A6J1H4P1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.0e-166
Identity = 266/324 (82.10%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 12  MMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIR 71
           MMIIFVRGNE E YR +G KK AW+T++VD++GHGNFSTIQAAIDSVPS+N FW+SIHI 
Sbjct: 3   MMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHIN 62

Query: 72  PGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNS 131
           PG+YREKVKIPYDKPYIILKG R++ TKVVW+DH T+AQSPTFTSSADN+VVKSI+FVNS
Sbjct: 63  PGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNS 122

Query: 132 YNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIF 191
           YN+PW NGN R PAVAAMITGDK+ FY+CGFYGVQDTLWD+QGRHYYHRC IQGAVDFIF
Sbjct: 123 YNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIF 182

Query: 192 GAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRP 251
           G+AQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFK+C VFGSGSA+LGRP
Sbjct: 183 GSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 242

Query: 252 WRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLS 311
           WR YSRVIF+NS+FSN+INP+GWDPWHFVG+E+QLTYAE  CYGPGSD+S RV WEKKL 
Sbjct: 243 WRGYSRVIFYNSSFSNVINPSGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKKLD 302

Query: 312 PKDIRQLTSMSFIDVEGWIQDQPW 336
             +I+QLT MSFID EGW+QDQPW
Sbjct: 303 LNEIQQLTDMSFIDTEGWMQDQPW 326

BLAST of IVF0006446 vs. ExPASy TrEMBL
Match: A0A6J1CDJ5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.4e-155
Identity = 257/337 (76.26%), Postives = 288/337 (85.46%), Query Frame = 0

Query: 1   MWTIIRGIFVL---MMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDS 60
           MW  +RGI  L   + IIF RG E +SYR +G KK AW+T++VD++GHGNFSTIQAAID 
Sbjct: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60

Query: 61  VPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSS 120
           VPS+NRFWVSI ++ G+YREKV IPYDKPYIILKG  KR T+VVWDDHL +AQSPTFTSS
Sbjct: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120

Query: 121 ADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHY 180
           ADNIVVK ISF NSYN PW NGNPRVPAVAAM+TGDKSSFY+CGFYG+QDTLWD  GRHY
Sbjct: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180

Query: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKE 240
           +HRCTIQGAVDFIFG AQSI++GC+ISVVGEA LP   T FITAQGRTDPND NGFVFK+
Sbjct: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEA-LPPRITGFITAQGRTDPNDGNGFVFKD 240

Query: 241 CNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPG 300
           C V GSGS +LGRPWR YSRVIF+NSNFSNIINPNGWDPW F G E QLTYAE  CYGPG
Sbjct: 241 CKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPG 300

Query: 301 SDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
           SD+S RV WEKKLS ++IRQLTSMSF+D EGW+Q+QP
Sbjct: 301 SDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336

BLAST of IVF0006446 vs. NCBI nr
Match: XP_008457276.1 (PREDICTED: probable pectinesterase 29 [Cucumis melo])

HSP 1 Score: 716 bits (1849), Expect = 3.25e-261
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60
           MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS
Sbjct: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60

Query: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120
           DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN
Sbjct: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120

Query: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180
           IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR
Sbjct: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180

Query: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240
           CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV
Sbjct: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240

Query: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI 300
           FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI
Sbjct: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDI 300

Query: 301 SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL 336
           SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL
Sbjct: 301 SRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL 336

BLAST of IVF0006446 vs. NCBI nr
Match: XP_011650140.1 (probable pectinesterase 29 [Cucumis sativus])

HSP 1 Score: 667 bits (1721), Expect = 1.11e-241
Identity = 314/337 (93.18%), Postives = 322/337 (95.55%), Query Frame = 0

Query: 1   MWTIIRGIF-VLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVP 60
           MW IIRGI  VLMM+IFVRGNEGE YRR+G KKFAWKTLIVDK GHGNFSTIQAAIDSVP
Sbjct: 1   MWIIIRGIILVLMMMIFVRGNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVP 60

Query: 61  SDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSAD 120
           S+NRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKR TKVVWDDHLTVAQSPTFTSSAD
Sbjct: 61  SNNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSAD 120

Query: 121 NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH 180
           NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH
Sbjct: 121 NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH 180

Query: 181 RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECN 240
           RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLP GSTSFITAQGRT+PNDANGFVFKECN
Sbjct: 181 RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPYGSTSFITAQGRTNPNDANGFVFKECN 240

Query: 241 VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSD 300
           VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPW FVGYEN LTY ENDCYGPGSD
Sbjct: 241 VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWQFVGYENHLTYVENDCYGPGSD 300

Query: 301 ISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPWL 336
           IS RVSWEKKLS K+I++L SM+FID EGWIQDQPWL
Sbjct: 301 ISGRVSWEKKLSWKEIKKLISMNFIDDEGWIQDQPWL 337

BLAST of IVF0006446 vs. NCBI nr
Match: XP_038875009.1 (probable pectinesterase 29 [Benincasa hispida])

HSP 1 Score: 633 bits (1633), Expect = 2.86e-228
Identity = 296/338 (87.57%), Postives = 317/338 (93.79%), Query Frame = 0

Query: 1   MWTIIRGIFV---LMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDS 60
           MW IIRGIFV   LMMIIFVRGNEG+SYR +G KK  WKTLIVD+NGHGNFSTIQAAIDS
Sbjct: 1   MW-IIRGIFVGLILMMIIFVRGNEGQSYRSVGMKKLTWKTLIVDQNGHGNFSTIQAAIDS 60

Query: 61  VPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSS 120
           +PS+NRFWVSIH++PG+YREKVKIPYDKPYIILKG  KR TKVVWDDHLTVAQSPTFTSS
Sbjct: 61  IPSNNRFWVSIHVKPGVYREKVKIPYDKPYIILKGQSKRTTKVVWDDHLTVAQSPTFTSS 120

Query: 121 ADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHY 180
           ADNIVVKSI+FVNSYNYPW NGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHY
Sbjct: 121 ADNIVVKSITFVNSYNYPWNNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHY 180

Query: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKE 240
           YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRT+PNDANGFVFKE
Sbjct: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTNPNDANGFVFKE 240

Query: 241 CNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPG 300
           CNVFGSGSA+LGRPWR YSRVIF+NSNFSNIINPNGWDPWHFVG ENQLTYAEN CYGPG
Sbjct: 241 CNVFGSGSAFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWHFVGQENQLTYAENGCYGPG 300

Query: 301 SDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQPW 335
           +D+S RV WEKKLS  +IRQLTS++FI+ E WIQDQP+
Sbjct: 301 ADMSHRVEWEKKLSLNEIRQLTSLNFINGESWIQDQPY 337

BLAST of IVF0006446 vs. NCBI nr
Match: KAE8651766.1 (hypothetical protein Csa_006597 [Cucumis sativus])

HSP 1 Score: 625 bits (1613), Expect = 1.17e-224
Identity = 301/341 (88.27%), Postives = 310/341 (90.91%), Query Frame = 0

Query: 1   MWTIIRGIF-VLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVP 60
           MW IIRGI  VLMM+IFVRGNEGE YRR+G KKFAWKTLIVDK GHGNFSTIQAAIDSVP
Sbjct: 1   MWIIIRGIILVLMMMIFVRGNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVP 60

Query: 61  SDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSAD 120
           S+NRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKR TKVVWDDHLTVAQSPTFTSSAD
Sbjct: 61  SNNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSAD 120

Query: 121 NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH 180
           NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH
Sbjct: 121 NIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYH 180

Query: 181 RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECN 240
           RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLP GSTSFITAQGRT+PNDANGFVFKECN
Sbjct: 181 RCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPYGSTSFITAQGRTNPNDANGFVFKECN 240

Query: 241 VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVG-------------YENQL 300
           VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPW F               + N L
Sbjct: 241 VFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWQFKKKFKNKGIEVVESKFRNHL 300

Query: 301 TYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVE 327
           TY ENDCYGPGSDIS RVSWEKKLS K+I++L SM+FID E
Sbjct: 301 TYVENDCYGPGSDISGRVSWEKKLSWKEIKKLISMNFIDDE 341

BLAST of IVF0006446 vs. NCBI nr
Match: KAA0033293.1 (putative pectinesterase 29 [Cucumis melo var. makuwa] >TYK14815.1 putative pectinesterase 29 [Cucumis melo var. makuwa])

HSP 1 Score: 602 bits (1553), Expect = 5.97e-217
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60
           MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS
Sbjct: 1   MWTIIRGIFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPS 60

Query: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120
           DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN
Sbjct: 61  DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADN 120

Query: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180
           IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR
Sbjct: 121 IVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHR 180

Query: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240
           CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV
Sbjct: 181 CTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNV 240

Query: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 283
           FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE
Sbjct: 241 FGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 283

BLAST of IVF0006446 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 5.4e-115
Identity = 188/300 (62.67%), Postives = 235/300 (78.33%), Query Frame = 0

Query: 38  LIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 97
           + VD++GHGNF+TIQ AIDSVP +NR W  I+++ GLYREK+KIPY+KP+I+L G  KR 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 98  TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWK---NGNPRVPAVAAMITGDK 157
           T+V WDDH +VAQSPTF++ ADN VVKSI+F NSYN+P K   N NPR PAVAA+I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 158 SSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLG 217
           S+FY  GF G+QDTLWD  GRHY+HRCTIQGAVDFIFG  QSI+Q C I V+G  L P G
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEP-G 213

Query: 218 STSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGW 277
              +ITAQGRT+P DANGF+F  C V+G+G A+LGRPWR YSRVIF+NSN ++++ P GW
Sbjct: 214 LAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 278 DPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
           D W+FVG+ENQL +AE+ C+G G++I RRV W KKLS   I+ L  +SFI+  GW++D P
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 332

BLAST of IVF0006446 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 2.0e-109
Identity = 182/300 (60.67%), Postives = 227/300 (75.67%), Query Frame = 0

Query: 38  LIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 97
           +IVD++GHGNF+TIQ AIDSVP +N  W  I+++ GLYREK+ IP  KP+I++ G  KR+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWK---NGNPRVPAVAAMITGDK 157
           T+V WDDH ++AQSPTF + ADN VVK I+F NSYN+P     N NPRVPAVAA I GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 158 SSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLG 217
           S+FY  GF G+QDTLWD+ GRHY+HRCTIQGAVDFI G+ QSI+Q C I V+G  L P G
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP-G 208

Query: 218 STSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGW 277
            T +ITAQGRT+ NDANGFVF  C V G G AYLGR WR YSRVIF+NSN +++++P GW
Sbjct: 209 VTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGW 268

Query: 278 DPWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
             W++ GYE QLTYAE+ C+G GS+ SRR  W KKLS   ++ L  +SFI+  GW++D P
Sbjct: 269 WEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVEDLP 327

BLAST of IVF0006446 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 9.7e-80
Identity = 145/299 (48.49%), Postives = 185/299 (61.87%), Query Frame = 0

Query: 36  KTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRK 95
           KT+IV+ N    F T+Q+AIDS+P  N+ W+ I I  G+Y EKV IP  K YI ++G   
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 96  RNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKS 155
             T + + DH     S TFTS   NI++  I+F N YN    + +P  PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 156 SFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGS 215
           +     F G QDTL+D+ GRHYY RC I G +DFIFG AQSIF+GC++ +      P   
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 216 TSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWD 275
              ITAQGR  P D  GFVFK+C V GSG A LGR W++YSRVIF+ S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 276 PWHFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
            W   G E  +T+ E  C G G+D S+RV W  K S KD+ Q T+++FID EGW+   P
Sbjct: 279 AWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWLSRLP 336

BLAST of IVF0006446 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.5e-75
Identity = 140/327 (42.81%), Postives = 194/327 (59.33%), Query Frame = 0

Query: 8   IFVLMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVS 67
           + +++M +FV G    +      ++ A+ T+ VD NG GNF+T+Q+AIDS+   N  W+ 
Sbjct: 12  VCMVVMTLFVYGYSAAA------EQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIR 71

Query: 68  IHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSIS 127
           +  + G+YREKV IP +K +I L+G     T + +DDH     S TFT+ AD+IV+  I+
Sbjct: 72  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 131

Query: 128 FVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAV 187
           F N+YN    N    VPAVAA + GD+       F G+QDTL+D +GRHYY RC I G +
Sbjct: 132 FKNTYNIVPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGI 191

Query: 188 DFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAY 247
           DFIFG  QS+F+ C++++      P      ITA  R  P+D  GFVF +C V G G   
Sbjct: 192 DFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTL 251

Query: 248 LGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPGSDISRRVSWE 307
           LGR W + +RVIF  S  S+++ P GWD W   G E  LT+ E  C G G+D S+RV W 
Sbjct: 252 LGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWL 311

Query: 308 KKLSPKDIRQLTSMSFIDVEGWIQDQP 335
           KKLS  ++    S+SFID +GWI   P
Sbjct: 312 KKLSLSEVDGFASVSFIDQDGWISRFP 331

BLAST of IVF0006446 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-64
Identity = 123/297 (41.41%), Postives = 174/297 (58.59%), Query Frame = 0

Query: 40  VDKNGHGNFSTIQAAIDSVPS--DNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRN 99
           VD++G G+FS IQ AI+S+P   +N     I ++PG+YREKV IP +KPYI L G +  N
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 100 TKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSF 159
           T ++W D   + +SPT T  A + V + ++  N +    +       AVA  +  DK++F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGR-------AVALRVAADKAAF 172

Query: 160 YRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTS 219
           Y C     QDTL D+ G HY+  C I+GA DFI G+A S+++ C +     +L P  +  
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSP--NNG 232

Query: 220 FITAQGRTDPNDANGFVFKECNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPW 279
            ITAQ RT   + +GF F  C + GSGS +LGRPW AYSRV+F  S FSN++ P GW+ W
Sbjct: 233 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 292

Query: 280 HFVGYENQLTYAENDCYGPGSDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 335
                EN + Y E  CYGPG+D  +RV W K+LS ++     S  FI  + W++  P
Sbjct: 293 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAP 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PSN07.6e-11462.67Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
Q3E9D32.8e-10860.67Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
O644791.4e-7848.49Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
Q4PSQ57.7e-7442.81Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q9SIJ92.1e-6341.41Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C5445.3e-203100.00Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1[more]
A0A5D3CTI51.1e-168100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040... [more]
A0A6J1KUQ13.6e-16783.02Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1[more]
A0A6J1H4P14.0e-16682.10Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1[more]
A0A6J1CDJ55.4e-15576.26Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008457276.13.25e-261100.00PREDICTED: probable pectinesterase 29 [Cucumis melo][more]
XP_011650140.11.11e-24193.18probable pectinesterase 29 [Cucumis sativus][more]
XP_038875009.12.86e-22887.57probable pectinesterase 29 [Benincasa hispida][more]
KAE8651766.11.17e-22488.27hypothetical protein Csa_006597 [Cucumis sativus][more]
KAA0033293.15.97e-217100.00putative pectinesterase 29 [Cucumis melo var. makuwa] >TYK14815.1 putative pecti... [more]
Match NameE-valueIdentityDescription
AT3G24130.15.4e-11562.67Pectin lyase-like superfamily protein [more]
AT5G18990.12.0e-10960.67Pectin lyase-like superfamily protein [more]
AT2G19150.19.7e-8048.49Pectin lyase-like superfamily protein [more]
AT2G47280.15.5e-7542.81Pectin lyase-like superfamily protein [more]
AT2G21610.11.5e-6441.41pectinesterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 38..325
e-value: 3.8E-68
score: 229.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 34..335
e-value: 1.1E-100
score: 339.1
NoneNo IPR availablePANTHERPTHR31321:SF76PECTINESTERASE 10-RELATEDcoord: 12..334
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 12..334
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 183..192
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 36..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0006446.2IVF0006446.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity