IVF0005006 (gene) Melon (IVF77) v1

BLAST of IVF0005006 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 6.2e-20
Identity = 67/208 (32.21%), Postives = 98/208 (47.12%), Query Frame = 0

Query: 36  TKP-RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSD---------- 95
           +KP + LCLHG+  S  +  K++G     +    +LHF   P  A+ ++D          
Sbjct: 2   SKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRL 61

Query: 96  --VEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAAL 155
             + G  +   F WF+       Y ++DE L  I  YM E GPFDG +GFSQGA + A L
Sbjct: 62  AALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAML 121

Query: 156 PGFQAKG---IALTKVPKIKFVIIVSGAKFRSESVAEKAYWTP-IGCPSLHFLGEEDFLM 215
                 G       + P  KFV+ V G  FR+E      ++ P +  PSLH  G  D L+
Sbjct: 122 AQMLQPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTLV 181

Query: 216 P--HGKKLLESYIEPTIITHPKGHTIPR 225
           P    K+L+E      ++ HP  H +P+
Sbjct: 182 PLARSKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of IVF0005006 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 90.1 bits (222), Expect = 3.8e-17
Identity = 62/199 (31.16%), Postives = 95/199 (47.74%), Query Frame = 0

Query: 37  KPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEW 96
           K + L LHG+R +A   K ++G +   +   ++  F+ AP PA     V G  DP    W
Sbjct: 11  KLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPDPNQRSW 70

Query: 97  FQFSPEFTEYR---------NFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 156
           + F+ +   ++          FDE L  +E      G   G LGFSQGA     L    A
Sbjct: 71  W-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEG-CHGLLGFSQGACFVGLLCDLSA 130

Query: 157 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW-TPIGCPSLHFLGEEDFLMPH--GKKL 216
           +G+   K    +F ++ SG  FRS S+    Y+   +  PSLH  GE D ++     + L
Sbjct: 131 RGMTTMKP---QFAVVASG--FRSGSLVHLNYYENKVQIPSLHIFGETDEIITKDMSEAL 190

Query: 217 LESYIEPTIITHPKGHTIP 224
            E++++P ++THP GH  P
Sbjct: 191 AETFLDPEVVTHPGGHYFP 202

BLAST of IVF0005006 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.1e-15
Identity = 69/217 (31.80%), Postives = 97/217 (44.70%), Query Frame = 0

Query: 37  KPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPF----------PAEGKSD-- 96
           K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  +  
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 97  ----VEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIENYMIEHGPFDGFLGFS 156
               V   FD P    +     QF P    T+   FD+ L++++    E GPFDG LGFS
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFS 472

Query: 157 QGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFL-- 216
           QGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH    
Sbjct: 473 QGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIFGS 532

Query: 217 --GEEDFLMPHGKKLLESYIE---PTIITHPKGHTIP 224
             G++  ++      L    E    TI+ H  GH IP
Sbjct: 533 QPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

BLAST of IVF0005006 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-15
Identity = 62/204 (30.39%), Postives = 88/204 (43.14%), Query Frame = 0

Query: 39  RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEGI---------F 98
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 99  DPPYFEWFQFS--------PEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAA 158
           + P   WF            E T  R  +E L  +   + + GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 159 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMP-- 218
           +      G    + P  +FVI+VSG   R   + E     P+  PSLH  G+ D ++P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 219 HGKKLLESYIEPTIITHPKGHTIP 224
              +L   +     +TH  GH IP
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

BLAST of IVF0005006 vs. ExPASy Swiss-Prot
Match: Q9D7E3 (Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-15
Identity = 65/204 (31.86%), Postives = 90/204 (44.12%), Query Frame = 0

Query: 39  RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPA------EGKSDVEGIFDP- 98
           R LCL GFR S    +++ G    ++  + +L  L  P P       EG     G   P 
Sbjct: 8   RVLCLAGFRQSERGFREKTGALRKTLRGRAELVCLSGPHPVPEAAAPEGSCPDSGPCSPE 67

Query: 99  --PYFEWFQ------FS--PEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAA 158
             P   WF       FS   E T  R   E L  +   +   GPFDG LGFSQGA L+A 
Sbjct: 68  EQPRGWWFSEEEADVFSALEESTVCRGLQEALETVARALDTLGPFDGLLGFSQGAALAAY 127

Query: 159 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMP-- 218
           +      G    + P  +F+I+VSG  F    + E    +P+  PSLH  G+ D ++P  
Sbjct: 128 VCALGQAGD--PRFPLPRFIILVSG--FCPRGLKEPILQSPMSLPSLHVFGDTDRVIPSQ 187

Query: 219 HGKKLLESYIEPTIITHPKGHTIP 224
              +L   ++    +TH  GH IP
Sbjct: 188 ESMQLASRFLGAVTLTHSGGHFIP 207

BLAST of IVF0005006 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.9e-141
Identity = 248/249 (99.60%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAY 
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

Query: 241 SKMLNETEE 250
           SKMLNETEE
Sbjct: 241 SKMLNETEE 249

BLAST of IVF0005006 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.0e-134
Identity = 236/249 (94.78%), Postives = 240/249 (96.39%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           M  FFPSLSSHQ L+TIPQF FPT+PIMGSDQK FTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           I+HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAY 
Sbjct: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFI RI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240

Query: 241 SKMLNETEE 250
           SK+LNE EE
Sbjct: 241 SKILNENEE 249

BLAST of IVF0005006 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.9e-125
Identity = 221/222 (99.55%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 28  MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAY TPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 250
           YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

BLAST of IVF0005006 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.4e-115
Identity = 206/249 (82.73%), Postives = 222/249 (89.16%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MSS  P L S    S  P+FH   +PIMG+D +I  KPRFLCLHGFRTS AILKKQVGKW
Sbjct: 1   MSSLSPLLCSPISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM
Sbjct: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           ++HGPFDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAY 
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
             IGCPSLHFLG+EDFLMPHG+KLLESYIEP II HPKGHTIPRLDD+ LEVMESFI++I
Sbjct: 181 PVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKI 240

Query: 241 SKMLNETEE 250
           SKML+E EE
Sbjct: 241 SKMLDEAEE 249

BLAST of IVF0005006 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.3e-115
Identity = 203/249 (81.53%), Postives = 223/249 (89.56%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MSS  P L S    +  P+FH   +PIMG+D +I  KPRFLCLHGFRTS AILKKQVGKW
Sbjct: 1   MSSLSPLLCSPISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM
Sbjct: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           ++HGPFDGFLGFSQGAILSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRS+SVAEKAY 
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           + IGCPSLHFLG+EDFLMPHG+KLLESYIEP +I HPKGHTIPRLDD+ LEVMESFI++I
Sbjct: 181 SAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKI 240

Query: 241 SKMLNETEE 250
           SKML+E EE
Sbjct: 241 SKMLDEAEE 249

BLAST of IVF0005006 vs. NCBI nr
Match: XP_008444882.1 (PREDICTED: dihydrofolate reductase-like [Cucumis melo])

HSP 1 Score: 509 bits (1310), Expect = 5.63e-182
Identity = 248/249 (99.60%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAY 
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

Query: 241 SKMLNETEE 249
           SKMLNETEE
Sbjct: 241 SKMLNETEE 249

BLAST of IVF0005006 vs. NCBI nr
Match: XP_004152614.1 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 485 bits (1248), Expect = 1.60e-172
Identity = 236/249 (94.78%), Postives = 240/249 (96.39%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           M  FFPSLSSHQ L+TIPQF FPT+PIMGSDQK FTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           I+HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAY 
Sbjct: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFI RI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240

Query: 241 SKMLNETEE 249
           SK+LNE EE
Sbjct: 241 SKILNENEE 249

BLAST of IVF0005006 vs. NCBI nr
Match: KAA0065116.1 (dihydrofolate reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 456 bits (1172), Expect = 2.31e-161
Identity = 221/222 (99.55%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 28  MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAY TPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 249
           YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

BLAST of IVF0005006 vs. NCBI nr
Match: KAE8652134.1 (hypothetical protein Csa_022239 [Cucumis sativus])

HSP 1 Score: 443 bits (1140), Expect = 1.74e-156
Identity = 215/222 (96.85%), Postives = 217/222 (97.75%), Query Frame = 0

Query: 28  MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQK FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAY TPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 249
           YIEPTIITHPKGHTIPRLDDKALEVMESFI RISK+LNE EE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 222

BLAST of IVF0005006 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 429 bits (1102), Expect = 1.92e-150
Identity = 209/249 (83.94%), Postives = 224/249 (89.96%), Query Frame = 0

Query: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60
           M+S FPSL ++             +PIMG+D KI  KPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MTSPFPSLHTNS-----------RIPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVLDQ DLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFS EFTEYRNFDECLSFIE+YM
Sbjct: 61  PASVLDQFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYM 120

Query: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYW 180
           ++HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVA+KAY 
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240
           TPIGCPSLHFLGEEDFLMPHG+KLLESY+EPT+I HPKGHT+PRLDDK LEVMESFI+RI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGQKLLESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRI 238

Query: 241 SKMLNETEE 249
           SK L+ETEE
Sbjct: 241 SKTLDETEE 238

BLAST of IVF0005006 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 302.0 bits (772), Expect = 4.5e-82
Identity = 140/223 (62.78%), Postives = 176/223 (78.92%), Query Frame = 0

Query: 28  MGSD-QKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVE 87
           MGS+ + I  KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 88  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQ 147
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MI+ GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 148 AKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLGEEDFLMPHGKKLLE 207
           AKGIA  KVPKIKFVII+ GAK +S  +AE AY + +   SLHFLGE DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 208 SYIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 250
           SY  P ++ HPKGHT+PRLD+K+LE + +FI  +  ++ E ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

BLAST of IVF0005006 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 6.9e-75
Identity = 129/214 (60.28%), Postives = 162/214 (75.70%), Query Frame = 0

Query: 38  PRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWF 97
           PR LCLHGFRTS  IL+  +GKWP ++L  LDL FLDAPFPA GKSDVE  FDPPY+EW+
Sbjct: 30  PRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYEWY 89

Query: 98  QFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPK 157
           Q +  F EYRNF+ECL++IE+YMI++GPFDG LGFSQGA L+AA+PG Q +G ALTKVPK
Sbjct: 90  QANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKVPK 149

Query: 158 IKFVIIVSGAK-----FRSESVAEKAYWTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPT 217
           +KF++I+SGAK     F     A  A+ +P+ CPSLHF+GE DFL   G+ L+ES++EP 
Sbjct: 150 VKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVEPV 209

Query: 218 IITHPKGHTIPRLDDKALEVMESFIQRISKMLNE 247
           +I H  GH IP+LD KA E M SF Q I +ML++
Sbjct: 210 VIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

BLAST of IVF0005006 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 239.2 bits (609), Expect = 3.6e-63
Identity = 113/166 (68.07%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 28  MGSD-QKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVE 87
           MGS+ + I  KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 88  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQ 147
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MI+ GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 148 AKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFLG 193
           AKGIA  KVPKIKFVII+ GAK +S  +AE AY + +   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of IVF0005006 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-16
Identity = 69/217 (31.80%), Postives = 97/217 (44.70%), Query Frame = 0

Query: 37  KPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPF----------PAEGKSD-- 96
           K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  +  
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 97  ----VEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIENYMIEHGPFDGFLGFS 156
               V   FD P    +     QF P    T+   FD+ L++++    E GPFDG LGFS
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFS 472

Query: 157 QGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYWTPIGCPSLHFL-- 216
           QGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH    
Sbjct: 473 QGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIFGS 532

Query: 217 --GEEDFLMPHGKKLLESYIE---PTIITHPKGHTIP 224
             G++  ++      L    E    TI+ H  GH IP
Sbjct: 533 QPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564