Homology
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match:
Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)
HSP 1 Score: 694.9 bits (1792), Expect = 7.2e-199
Identity = 352/554 (63.54%), Postives = 428/554 (77.26%), Query Frame = 0
Query: 5 EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
E+WCVVTGGRGFAARHLVEML++Y MF VRI DL +I L+ E G L E LRSGR QY
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68
Query: 65 VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
+SADLRDK+QV+KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+ VKRL
Sbjct: 69 ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128
Query: 125 IYTSSASVVFDGINALINTDESMPY------------------ASKANGTNGLLTCSLRP 184
IYTSS SVVFDG++ ++N ESM Y KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188
Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248
Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308
Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI+RTI+++SHL A + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368
Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVA 424
REGPSKAS L GG AD LLWKD ++TLIA+ +L++IYYNF+A T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428
Query: 425 LIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGND 484
+FLF+HG LPEKI+GY VEK+ AS F LS++ S +++++ S WN+ V+ +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488
Query: 485 WVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-R 540
W KV LLALS G +SL +++ + AF F +YEKKE+EIDS+ F+ + +
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACK 548
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match:
Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)
HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 279/445 (62.70%), Postives = 345/445 (77.53%), Query Frame = 0
Query: 5 EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
E+WCVVTGGRGFAARHLVEML++Y MF VRI DL +I L EE G LGEA+RSGR QY
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68
Query: 65 VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G NVID+C+E VKRL
Sbjct: 69 VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128
Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
IYTSS SVVFDG++ +N DES+PY KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188
Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
S IFGPGD+L+VPSLV ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248
Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
+AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308
Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
YGM VP TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368
Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIA---PECTMITALSKLL 424
E + + W K++TLIA+++L+ +Y+NF+A +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428
Query: 425 MVALIFLFIHGSLPEKIYGYRVEKL 429
+V+ IF+FI+G LPEK+ + +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match:
A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)
HSP 1 Score: 374.4 bits (960), Expect = 2.2e-102
Identity = 221/537 (41.15%), Postives = 314/537 (58.47%), Query Frame = 0
Query: 3 SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
S K CVV GGRGF R LV L++ ++VR+ D G ++ L+ E L +AL SGRA
Sbjct: 10 SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69
Query: 63 QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
Y D+RDK Q++K EG VVF+M A + ++Y Y V V+G +NVI +C E V+
Sbjct: 70 SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129
Query: 123 RLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTCSL 182
+LIY S+A VVFDG + + DES+ K AN +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189
Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
R S +FGPGD VP LV A++G +KFI+G G N DFTY ENV HAH+CA +AL +
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249
Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
E AG+ +FITN++P++FW+FVS I++GLGY RP IK+P +V+ + ++ WT++
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309
Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEH 362
G+ T + LL S RTFN + AK L YTP+V+L++GI T++ +S E
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369
Query: 363 EPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLL 422
S A L G ADILLW++++KT ++ +VL YY F T ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429
Query: 423 MVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSR 482
+ + L+ +P KI+G++V K+ F +SE R ++ + WN VR+FKSLS
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489
Query: 483 GNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 521
G DW+ K+A SL L I SL TV+ F+FTGFFIYE+ E E+ L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match:
Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)
HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 131/359 (36.49%), Postives = 193/359 (53.76%), Query Frame = 0
Query: 3 SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
S+ K C V GG GF +H+VE L++ ++V + D+ + + R
Sbjct: 24 SKAKKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD---------------NPRV 83
Query: 63 QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
Q+ DL ++ + A +GV VFH A+P NN +L Y VN G K VI++C E V+
Sbjct: 84 QFFIGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQ 143
Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKA-------------------NGTNGLLTCS 182
+LI TSSASVVF+G++ + N E +PYA K + LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203
Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
+RP GIFGP D LVP L+ AR GK KF+IG+G N DFT+VENV H H+ A L+
Sbjct: 204 IRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLSQD 263
Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
+ G+A+ ITN EPI FW F+S IL GL YE P+ IP + +A +++ +
Sbjct: 264 AAL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMV 323
Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 343
+ P P FTP R+ L ++ KAK Y P+V++ E ++RT++++ HL+ +
Sbjct: 324 VSPLIQIQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match:
Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)
HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 131/359 (36.49%), Postives = 194/359 (54.04%), Query Frame = 0
Query: 3 SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
S+ K C V GG GF +H+VE L+ ++V + D+ + + R
Sbjct: 24 SKAKKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD---------------NPRV 83
Query: 63 QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
Q+ DL ++ + A +GV VFH A+P S+ NN +L Y VN G K VI++C E V+
Sbjct: 84 QFFIGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQ 143
Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKA-------------------NGTNGLLTCS 182
+LI TSSASVVF+G++ + N E +PYA K + LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203
Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
+RP GIFGP D LVP L+ AR GK KF+IG+G N DFT+VENV H H+ A L+
Sbjct: 204 IRPHGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSRD 263
Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
+ G+A+ ITN EPI FW F+S IL GL YE P+ IP + +A +++ +
Sbjct: 264 AGL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMV 323
Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 343
L P FTP R+ L ++ KAK + Y P+V++ + ++RT++++ HL+ +
Sbjct: 324 LSPLIQIQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361
BLAST of IVF0004486 vs. ExPASy TrEMBL
Match:
A0A1S3BDP7 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)
HSP 1 Score: 1050.0 bits (2714), Expect = 3.3e-303
Identity = 539/557 (96.77%), Postives = 539/557 (96.77%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
VKRLIYTSSASVVFDGINALINTDESMPYAS KANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
BLAST of IVF0004486 vs. ExPASy TrEMBL
Match:
A0A0A0KDI8 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G361340 PE=4 SV=1)
HSP 1 Score: 1027.7 bits (2656), Expect = 1.7e-296
Identity = 526/557 (94.43%), Postives = 533/557 (95.69%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
VKRLIYTSSASVVFDGINALINTDESMPYAS KANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HE KREGPSKAS+YLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
RGNDW+LLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
BLAST of IVF0004486 vs. ExPASy TrEMBL
Match:
A0A6J1KG57 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1)
HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 487/556 (87.59%), Postives = 516/556 (92.81%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLI MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3 MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
VKRLIYTSSASVVFDGIN L+N DE++PYAS KANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
L+VA IFLFIHGSLPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
RGNDWV LLKV+VSLLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542
BLAST of IVF0004486 vs. ExPASy TrEMBL
Match:
A0A6J1G542 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1)
HSP 1 Score: 962.2 bits (2486), Expect = 9.0e-277
Identity = 486/557 (87.25%), Postives = 517/557 (92.82%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLI MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 3 MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGA+NVID+CVE+N
Sbjct: 63 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGARNVIDACVEKN 122
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
VKRLIYTSSASVVFDGIN L+N DE++PYAS KANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y
Sbjct: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
L+VA IFLFIHG LPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGILPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
RGNDWV LLKV+V+LLALSFIG+VSLQTLYKTVVLFAFT FFIYEKKE+EIDSLCQ+FQ
Sbjct: 483 RGNDWVRLLKVSVALLALSFIGLVSLQTLYKTVVLFAFTVFFIYEKKEQEIDSLCQKFQF 542
BLAST of IVF0004486 vs. ExPASy TrEMBL
Match:
A0A1S3BDV0 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)
HSP 1 Score: 962.2 bits (2486), Expect = 9.0e-277
Identity = 494/512 (96.48%), Postives = 494/512 (96.48%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
VKRLIYTSSASVVFDGINALINTDESMPYAS KANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 495
RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 512
BLAST of IVF0004486 vs. NCBI nr
Match:
XP_008445550.1 (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo])
HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 539/557 (96.77%), Postives = 539/557 (96.77%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
VKRLIYTSSASVVFDGINALINTDESMPYASK ANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
BLAST of IVF0004486 vs. NCBI nr
Match:
XP_011657360.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis sativus] >KGN47573.1 hypothetical protein Csa_018962 [Cucumis sativus])
HSP 1 Score: 1029 bits (2661), Expect = 0.0
Identity = 526/557 (94.43%), Postives = 533/557 (95.69%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
VKRLIYTSSASVVFDGINALINTDESMPYASK ANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HE KREGPSKAS+YLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
RGNDW+LLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
BLAST of IVF0004486 vs. NCBI nr
Match:
XP_038884192.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884193.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884195.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida])
HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 505/557 (90.66%), Postives = 526/557 (94.43%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGILGEALRSG 60
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQVLKAFEG EVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVEQN
Sbjct: 61 RAQYVSADLRDKAQVLKAFEGAEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEQN 120
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
VKRLIYTSSASVVFDGIN L N DESMPYASK ANG++GLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINGLTNADESMPYASKHNDFYSATKAEGEALVLKANGSSGLLTC 180
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNAYDFTYVENVAHAHVCAERALAS 240
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GG+ISE+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGIISEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTF+SSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFDSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
HE K+EGPSKAS YLRGGTAADILLWKDKRKTL+AL+VL+AIYYNFIAPE TMITALSKL
Sbjct: 361 HEHKKEGPSKASAYLRGGTAADILLWKDKRKTLVALLVLVAIYYNFIAPESTMITALSKL 420
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
L+VALIFLFIHGSLPEKIYGYRVEK+S+SCFSLSEERSRSIAIT+TSFWNSF+R+FKSLS
Sbjct: 421 LLVALIFLFIHGSLPEKIYGYRVEKISSSCFSLSEERSRSIAITVTSFWNSFIRHFKSLS 480
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
RGNDWVLLLKVAVSLLALSFIGV+SLQTLYKTVVLFAFTGFF+YEKKE+EIDSLCQRFQ
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVISLQTLYKTVVLFAFTGFFLYEKKEQEIDSLCQRFQA 540
BLAST of IVF0004486 vs. NCBI nr
Match:
XP_022999664.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999665.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999667.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999668.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima])
HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 487/556 (87.59%), Postives = 516/556 (92.81%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLI MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3 MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
VKRLIYTSSASVVFDGIN L+N DE++PYASK ANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
L+VA IFLFIHGSLPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 538
RGNDWV LLKV+VSLLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542
BLAST of IVF0004486 vs. NCBI nr
Match:
XP_023546590.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546591.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546592.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546593.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 486/556 (87.41%), Postives = 516/556 (92.81%), Query Frame = 0
Query: 1 MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
MGSEEKWCVVTGGRGFAARHLVEMLI MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3 MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62
Query: 61 RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63 RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122
Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
VKRLIYTSSASVVFDGIN L+N DE++PYASK ANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182
Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242
Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302
Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362
Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422
Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
L+VA IFLFIHG LPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGILPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482
Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 538
RGNDWV LLKV+V+LLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ
Sbjct: 483 RGNDWVRLLKVSVALLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542
BLAST of IVF0004486 vs. TAIR 10
Match:
AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )
HSP 1 Score: 694.9 bits (1792), Expect = 5.1e-200
Identity = 352/554 (63.54%), Postives = 428/554 (77.26%), Query Frame = 0
Query: 5 EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
E+WCVVTGGRGFAARHLVEML++Y MF VRI DL +I L+ E G L E LRSGR QY
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68
Query: 65 VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
+SADLRDK+QV+KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+ VKRL
Sbjct: 69 ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128
Query: 125 IYTSSASVVFDGINALINTDESMPY------------------ASKANGTNGLLTCSLRP 184
IYTSS SVVFDG++ ++N ESM Y KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188
Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248
Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308
Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI+RTI+++SHL A + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368
Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVA 424
REGPSKAS L GG AD LLWKD ++TLIA+ +L++IYYNF+A T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428
Query: 425 LIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGND 484
+FLF+HG LPEKI+GY VEK+ AS F LS++ S +++++ S WN+ V+ +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488
Query: 485 WVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-R 540
W KV LLALS G +SL +++ + AF F +YEKKE+EIDS+ F+ + +
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACK 548
BLAST of IVF0004486 vs. TAIR 10
Match:
AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )
HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-163
Identity = 293/471 (62.21%), Postives = 360/471 (76.43%), Query Frame = 0
Query: 88 MAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRLIYTSSASVVFDGINALINTDESM 147
MAAP+SSINN++L YSVNV+G +NVID+CV+ VKRLIYTSS SVVFDG++ ++N ESM
Sbjct: 1 MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESM 60
Query: 148 PY------------------ASKANGTNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGK 207
Y KANG NGLLTC +RPS IFGPGDRLLVPSLVA ARAGK
Sbjct: 61 AYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGK 120
Query: 208 SKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVISERAAGQAYFITNMEPIKFWEFV 267
SKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +S +AAGQAYFITNMEPIKFWEF+
Sbjct: 121 SKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFM 180
Query: 268 SLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGPYGMPVPQFTPSRIRLLSCCRTFN 327
S +LDGLGYERP IKIPA I+MPIAH+V TYK+LGPYGM VPQ TPSR+RLLSC RTF+
Sbjct: 181 SQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFD 240
Query: 328 SSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPKREGPSKASIYLRGGTAADILLWK 387
S+KAKDRL Y P+V LQEGI+RTI+++SHL A + KREGPSKAS L GG AD LLWK
Sbjct: 241 STKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWK 300
Query: 388 DKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVALIFLFIHGSLPEKIYGYRVEKLS 447
D ++TLIA+ +L++IYYNF+A T++TALSK L+VA +FLF+HG LPEKI+GY VEK+
Sbjct: 301 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 360
Query: 448 ASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGNDWVLLLKVAVSLLALSFIGVVSLQ 507
AS F LS++ S +++++ S WN+ V+ +SL +GNDW KV LLALS G +SL
Sbjct: 361 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 420
Query: 508 TLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-RSKCSVVGKVSTSKE 540
+++ + AF F +YEKKE+EIDS+ F+ + + K V K+ SK+
Sbjct: 421 SIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSDVYEKLFGSKK 471
BLAST of IVF0004486 vs. TAIR 10
Match:
AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )
HSP 1 Score: 553.5 bits (1425), Expect = 1.8e-157
Identity = 279/445 (62.70%), Postives = 345/445 (77.53%), Query Frame = 0
Query: 5 EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
E+WCVVTGGRGFAARHLVEML++Y MF VRI DL +I L EE G LGEA+RSGR QY
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68
Query: 65 VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G NVID+C+E VKRL
Sbjct: 69 VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128
Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
IYTSS SVVFDG++ +N DES+PY KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188
Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
S IFGPGD+L+VPSLV ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248
Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
+AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308
Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
YGM VP TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368
Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIA---PECTMITALSKLL 424
E + + W K++TLIA+++L+ +Y+NF+A +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428
Query: 425 MVALIFLFIHGSLPEKIYGYRVEKL 429
+V+ IF+FI+G LPEK+ + +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438
BLAST of IVF0004486 vs. TAIR 10
Match:
AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )
HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 252/366 (68.85%), Postives = 296/366 (80.87%), Query Frame = 0
Query: 5 EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
E+WCVVTGGRGFAARHLVEML++Y MF VRI DL +I L EE G LGEA+RSGR QY
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68
Query: 65 VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G NVID+C+E VKRL
Sbjct: 69 VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128
Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
IYTSS SVVFDG++ +N DES+PY KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188
Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
S IFGPGD+L+VPSLV ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248
Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
+AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308
Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 353
YGM VP TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368
BLAST of IVF0004486 vs. TAIR 10
Match:
AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )
HSP 1 Score: 374.4 bits (960), Expect = 1.5e-103
Identity = 221/537 (41.15%), Postives = 314/537 (58.47%), Query Frame = 0
Query: 3 SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
S K CVV GGRGF R LV L++ ++VR+ D G ++ L+ E L +AL SGRA
Sbjct: 10 SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69
Query: 63 QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
Y D+RDK Q++K EG VVF+M A + ++Y Y V V+G +NVI +C E V+
Sbjct: 70 SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129
Query: 123 RLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTCSL 182
+LIY S+A VVFDG + + DES+ K AN +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189
Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
R S +FGPGD VP LV A++G +KFI+G G N DFTY ENV HAH+CA +AL +
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249
Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
E AG+ +FITN++P++FW+FVS I++GLGY RP IK+P +V+ + ++ WT++
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309
Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEH 362
G+ T + LL S RTFN + AK L YTP+V+L++GI T++ +S E
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369
Query: 363 EPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLL 422
S A L G ADILLW++++KT ++ +VL YY F T ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429
Query: 423 MVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSR 482
+ + L+ +P KI+G++V K+ F +SE R ++ + WN VR+FKSLS
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489
Query: 483 GNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 521
G DW+ K+A SL L I SL TV+ F+FTGFFIYE+ E E+ L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q67ZE1 | 7.2e-199 | 63.54 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... | [more] |
Q9FX01 | 2.6e-156 | 62.70 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... | [more] |
A9X4U2 | 2.2e-102 | 41.15 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... | [more] |
Q9R1J0 | 3.0e-59 | 36.49 | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=1... | [more] |
Q5PPL3 | 3.0e-59 | 36.49 | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BDP7 | 3.3e-303 | 96.77 | Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1 | [more] |
A0A0A0KDI8 | 1.7e-296 | 94.43 | Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G361340 PE=4 SV=1 | [more] |
A0A6J1KG57 | 3.1e-277 | 87.59 | Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1 | [more] |
A0A6J1G542 | 9.0e-277 | 87.25 | Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1 | [more] |
A0A1S3BDV0 | 9.0e-277 | 96.48 | Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_008445550.1 | 0.0 | 96.77 | PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis ... | [more] |
XP_011657360.1 | 0.0 | 94.43 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis sativus] >K... | [more] |
XP_038884192.1 | 0.0 | 90.66 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_03... | [more] |
XP_022999664.1 | 0.0 | 87.59 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022... | [more] |
XP_023546590.1 | 0.0 | 87.41 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pep... | [more] |
Match Name | E-value | Identity | Description | |
AT2G26260.1 | 5.1e-200 | 63.54 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 | [more] |
AT2G26260.2 | 1.7e-163 | 62.21 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 | [more] |
AT1G47290.2 | 1.8e-157 | 62.70 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 | [more] |
AT1G47290.1 | 1.2e-145 | 68.85 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 | [more] |
AT2G43420.1 | 1.5e-103 | 41.15 | 3-beta hydroxysteroid dehydrogenase/isomerase family protein | [more] |