IVF0004486 (gene) Melon (IVF77) v1

Overview
NameIVF0004486
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionReticulon-like protein
Locationchr03: 21446667 .. 21456518 (+)
RNA-Seq ExpressionIVF0004486
SyntenyIVF0004486
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAGCGAGGAGAAATGGTGCGTGGTCACGGGAGGGAGAGGCTTCGCCGCACGACATCTCGTTGAAATGCTGATCAAATACAACATGTTTTCCGTTCGAATCGTTGACTTGGGCTCTTCCATTGAATTGGAAGCAAGCGAGGAGCAAGGGACTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAGTACGTTTCAGCGGATCTTCGGGACAAAGCTCAAGTGCTCAAAGGTTTGTGATTCTTTTAAAATCTTCGTGGCAGCGCTAATTATCTCTGTTCTATTTCTGAAAAGTAATTGCAATGTCAACTTGAATGTTGCAGCGTTCGAAGGAGTGGAGGTTGTTTTCCACATGGCTGCTCCGAATTCATCTATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGTACGAATCATTTGGTTTAGCAACTCTGTTTTTGATGTGTTGTTTATTATTATTGTTTTCTTTGCCTTCCAGGAGCTAAGAATGTAATTGATTCTTGTGTCGAGCAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTATTTGATGGGATAAATGCACTTATCAATACGGATGAGTCAATGCCCTACGCATCCAAGGTACTAACTCCACCGACCATCCAGGGGATTTATGTTCAGCTCATCAATTATTGGACTTGGTTTATTTTCATCGCTTGTTTGAAATTTGCATTTACTGTCTTGTCTCTTGGATATTGTTTGTCATCTGTTTCAGCATAATGATTTCTACTCTGCTACTAAAGCTGAGGGGAAGCTTTGGTTCTCAAGGCAAATGGCACAAATGGACTTCTAACGTGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTCGCCAATGCAAGGGCAGGGAAATCCAAGGTAAGCATGTTCCTTATTTTTGTTAGTTTTTTCTGGTTTTTTTTAAAAGTAAGAAACCACACTTTGGGACAATCTGTCCTCCATGCCATCAAGTGAATGAAGCTTGGAGAGAAAGGAATCCTCTCAAGCTGCAATATTACCAGAAATGACACAAGGATTAATTAAAATTCTTGTAACTTTTCATTTTCCTTTTAAGACAAATTTATTATAGCATTTGAGCTATAAAACAAAATAGCATTTGATTTGATGCTCTCTTCAAGATCCTGTAAATATTTTGTTTTTTTTTTTTGTTAACAAGAAACTACATTTTATTGATAAATTAAAAGGAAGATGAGTGTTCAAGGATCTGAGTTCTAAAGGGAATGAAGAAAAAAATTAAAAAAACAGGCTTACGTACATGCATGGAAAGTCTATACAGTTTTCTTTCTATTGCCCTAAGCAAATAAATTTCATTAAAAAAAAAAACAAAAATATGGCCTTCATTGTAATTGTCATATGTTTTAAATTTTACAGTTCATTATTGGTGATGGCAATAACACCTATGATTTTACTTATGTGGAAAACGTGGGGCATGCCCATGTATGTGCTGAACGAGCTCTAGCTGCAGGAGGTGTAATTTCAGAAAGAGCTGCAGGGCAGGTACCTTTTAAGATTTTTAAAAATATTGGGACAATGTGTCAGGGTGTGGAAGGATTTATTTATGTTGAGTTATTTTTGTCGTCTCTGTCTAATGAATAATCAAAACATATTATTCATATTGTAAGCTAAGCATGACATATCTAACTTTTCCTATCGTACAAAGGAATTTGTAGAAGTTGCGTATATTATGTAATGGATGATCAAACCAAAATCATAGTTTCCTCACATTTAGTTCTTGGGTATAAAACCATTGGAATGTGAATGTTATGCTTGCCTCTTGTCTTTGAAACTCTCATTATCATAGTTTTCACTGTGCACGTGGAACCAGTCTAGCAGTAAATTAATATCTTGAGCATATGTGTTCAGTATTTGCCTGTGAGCATGGAAATTGTGATGTTAATGTGTTCACTGCAGATCTTTTAATCGTGATTTAATGCATTAAACTCAGGAATCTCATGAATGTTAACGCATAGTTTAGGATTGTTGTAGCTTTTAAAGTAATCACTGTCAAATTTATTTCTAAAAAAGTAAGCTGTTCAGTAAATTTCTAAGTGATTTGTTAAAAAGTAAAAATGAAACAGGTACTTCTAAAAGTTGGCTAGAATGTCTAGGGATTCAATCTCAAAAGGAACTGTTAACCTGGTTTTGCAGTCAATGTAATTGGAAAAAAGGATTTTCTTTAAATTAATTTATAATCCGTTTTTTCAAACACTAGAAACAACTAAACAATTTCATTAAAGTATGAAATTCGCAAAAAGGATGTGAGTATGAAGCATGGTATTCAAAAAGGATTTCACAATAGGCATTTCCAATTTCTTAGAACGTAAGTCAAAGCAAGCTTAATTCAATGGTAGTTTACATGACCTTCTATCCTAGAGGTGAGAGGTCTGATCCCCTATCCCCATAATTGTTAGTGGAAAAGCGTATCAGTACGAACCAAAACGATGAGAACTTTTTCAGTAGAAAATTGCATACTCCTACTCACTTTTAGACTAGCAAAATCAATTCTAAAATGGAGTTCTAGGACATGTTTAGGAGTGATTCTGATATGGTTAAAATGACTTTTGTCATTTTCAAAATCACTCTAAAATATGCTTCAAATAGTTCAAACAAATTTTGATGTGAAAATGGCATTCAAAGGTGTAAAATTAAGTATTAAATTAATTTTAATTGATAAAACGTTGGTTTTAGAGTGATTTTGAATGTGAAAAAAGTGCTTTTAGCTTCTAAAAAATAACTCTTAGACATGCATAATATAAAATTGAATGGGTTTTTGCAGGCTTATTTCATAACCAACATGGAACCCATCAAGTTCTGGGAGTTTGTCTCTCTTATTCTGGATGGTCTTGGCTATGAAAGGTAATCTTTCTTCTAATTTAAGCTCTAGTCACTCTGATCACATGGCATTCCAGTCATCCTTTTCCATGTTTTGGTAAAGCAAAGATCTGTAATATCGAGAACTTTCATTCTGAGTTGCAGGCCAAGAATAAAGATCCCTGCCTGCATAGTGATGCCAATTGCACATATGGTGAACTGGACTTACAAGCTTTTAGGTCCTTATGGGATGCCTGTCCCACAGTTTACACCTTCCCGTATAAGACTTCTCTCTTGTTGTAGAACTTTTAATTCCTCAAAAGCCAAGGATCGACTTTTCTACACCCCCATTGTTTCACTTCAGGTTTTTTATGTTTCATTAATTCCATATACTTTTTGGCTTGTGCATGTTACTCCATTTCTTAACTCTTCAAGATACTCTTGGAATGCCTTCCATTAATGATTTTTCTGTAGAAACTAAAGAAAACTGTTTGTTTATTTTATTTTATTTTTATTTTTAAAAGGTCTTTTTAAGTACAACTTGGGGTGGGAGGATTTGAACCCGGGACCTCACGTCCTTGGGAACATACAAATGCTAGTTAAGCTAAGCTCACGTTGACAAACCATGCATATTTTGCTCTATTGAAAATCAAGATATGGACATTTCATAATCTGAAGATTTAAGTCTATATTTTATATAGGCCTTTTTGTACTTCCATATTTCTTTTATGGAAACTTATTGATTATTAAGAAAAAGGGGAGAGTGATGGCTTGTCCAAGTTGAACTTAAAGTGGAAGTGTGATTATATTTCCTCCCATTGGATCAGTTTCACGGGCCATATGATGTTTCTGGACATTTTATTATGTTAAATTTAATTTTCTTGTCCACATACAAATGTTCTTGCTACAGTCCATTGTCCAATTCTTGTAATGAAGGCCCTCTCGAACTAACATAAGGTGGGGTGAGTTTTTGTGGACTTCTGAAGGGAGGATTAATATTCCTTGTAGCTTTACTTTCTCCATAATGAAAAGTTTTTGTGTGTTCATGTCTTTTTTTTTTCCTTCTTTTTTAATGAATAAAAAAAAACTTATGTTAGGATTATATTTACTGTGCATATGTAACCTCTGCTCTCTCTATTTCTGTTGCTGTTTGTCTAATTATAGCAAGACATGTAGTTGTATCTTTCTCCTTGAATATCTATAAAATGATCAATGTGCTCTGGGTGGACCATTAACATTATTCCTCTGCCTCTTTCTCTCTTATACCAGTCGTCCATCTTGAACTAGTATCTTATATGCTCTCGATAATATTGACTACCATCACTATTATAAAATTTGAATATGGGTGTAGTAGACTTTGCCTCTAGCTGGTTATTTAGAAATTCACTACAGTCAAGCATGCTTACATAGCCATGCGATGCTGTATGTTCATGATTTGAGCTTATTGCTTAAAATGCCACGGTTATTCTTGACAGGAAGGGTCTACAGACGACATGTTTTATGAATGCCCATGGCCATGATGGCACCTGCACCATCAATTAATTTGAATATGAAGTGTTACTGCATTAGTCTTAGCTTTAGCTGCTCACTTCACAATTCACTATATTTAAACATGTTTACATAGTTATGTGATGTGTGAACGTCCATATCAGTGCTTGCAATGCAATAGTTATACTTCAAACAAGCCATAACACAGTATAGGAGCGTCCACCGTTGCCAATTAATATGAGTATGGAGTATTACTGTACTTGTCTTAGCTTGCTATCCGATAAATAAGCTATTCACCATATTTAAGCATGCTTGCATAGCTATTTGATGCTTTGTACGTCCATGATTGATGCTTAAAATGCAGTAGTTCTTCTTCACAGGAGGGTATACAAAGGACAATTGAAGCCTACTCCCATTTAAAGGCTGAGCATGAACCTAAAAGAGAAGGGCCATCCAAAGCCTCAATATACCTCAGGGGAGGAACAGGTGTGTGCTTCTTCTTTTTTAATATATAAATAAATTAATTTACTTTTTATATAGAAGAATTTTGAGGGGTTATTTGATTTCCTCTATACTAGTTTCCCAGCAATGGTTTGCCTCCATTCATTGTAAAAATCACAATGAATAATTAAGCAATTCATCGTCTTTTTATCCTCTTTATATAAATTACAGGTTTAAGAAGAAATATGTAGTTTACAAATTTTTTTTTTGTTTATTTTCAAATATAGTAAAATGAACAAAGATATAGCAAAATATCACAGCTTATCACTGATAGAATGTGATATTTTGCTATATTTGTATATATTTTCAGAAGTTTTGTCATTTAAAATAATTCTCCTTTTTTAATTCCATCCTGCATTTAAGCTCTTATAAGCCTTGCCTTGGACAAGTGACTTTAAAAAGGATTTATCCTTCTTTGGTCTGTTACTACTTACTATATTGTAGGCACATTATTTATTATTTATTATTTATTTTATTTTATTTTTTCCGTATATGATCATCATTTTTGTGTGCCCGTATATTCTCTCGTTTTTATCCCAATGAAAGTTGTTTTTAAAAAATTAAAAAAACAAAAATAAAAGATAGAAAGGGAAAATTTTATGGACATCAATCAATGAGAATGAATTTCCACTTTTCTACTTGGAAGTTGGAATTTATAAATCATTTTACTGGTGGCGTAGGGGAAGGCTAAACAATTTTATTTGTAGGGTCAAATAAAATGAAATAAATAAATTTAGAATGTGTTAAATATAAATTTTTTATCGATCCAACTTTAATGCTTTGAATTTAAGTTTACCGCTTCAATTTTAATAGACTAAGTTACAGACATTTTATTCCATGTCAATAATTTTATTAAAAGAAATTAATATATGATGGATTAAGTTGCAAGTTTAGTTTTTGAACTCTCCAGTGTTTGTCTAATAGGTTTATGAACTTAAAGAAGTATCTGATAAGACTCGAAACATTCATTTTGTATCTTATTGGTGCATGCTATTAATCTCATCAAACTCTGTTTGTCATTGATAATTGCCACTGGTCAAATGGTCTATGGATGAGAATTGCAATACCTCTTTTATTTGCAGGATGGGGAGTGGGTGGAGAAATAAAAATACAATAGGTCCCTTGGTCGGATTGAGAATGAAATAGTGGAAGAGGTTCTCTTGTTCTTCTCTGACCTCTACGACCCAGAAGTGTCTCCTAAACTGTGTGTTCAAGAAAGCGGTTGGAGTTCTTTTATCCAAAACGGAAGCGATTAGACTTTCTTGAACCCCTTTTAATTTGGAGAAGAGAAAAAAAGTAGTTAGTCTCATTCCTCGTGTTATAACATCCTTGCAAAAGTTCTAGCCAATCGTTTGAAAAAAATTCTCCCACTGTCGGTGTTGACTCGTAACTAGCGTTTTATAGTGGGGAGACAGATCTTTGATGAAGCTCTTGTAGCCAATGGAGCTATTAAGGATTACAGATTCAAAAAGAAATAATGAGTGGCTTTAAAATTTTATTTTGAGAAGCCCTATGATCATGTCTATTGGAATTTTTTGGACAGGGTGATGGAGAAGAAAGGTTTTGGGTATAAATAGGATTGGAATGTGGAGTTGTACTAGAACTGTGGGCTACTTTATTTAATGGGAGTCCGAGAGGCAAGATAAAGGATTCTAGGTGCCTTAACATGGTGACCCTTAATCACATTTTCTCTTATAGTTTGTGATGGATGTGCTGAATAGGATCACTTTTTGGGGTGTTGAAGGTTATTGATCCTTTTGAGGTTGAGGCTGGTAAAGAAAAAGTGACTTTATCTCACCTTTGGTTTGCCAATGCCGAGAACTTCATTTGGTCGTTGGAGGATGATAGGATCACAGGGGAATAGAGTTAAGGAGATTGGGACTTTTTTTGTCAGTTAGGCTCGGCTGAGGAAATGCAACTAATTGTTGGAGAAGTTTCTTTCCCATCCTCTCTTTTGTGAAAAACAAAAAGCTTTACATATTTTTCAAGATAAATTAAAACTTGAATACTAGTTATAGATAAATTTAATAAAAACGTCAGGAATCTAATTCTGATTCAGAAGAATCTGATTTTTTTCTCTTGTTCGTATTTGCACACTAATCCAATTCCTAATTGTGGACTTAACTTGTACGTGCGGAAAAATTGATAACCAATAATCATTACTGCTGTGTTCCTTTTGGCAGCTGCTGACATTTTACTCTGGAAGGATAAAAGGAAGACATTGATAGCATTGATAGTTTTGATGGCTATTTATTATAACTTCATTGCTCCCGAGTGTACCATGATCACTGCACTGTCAAAGCTTCTTATGGTGGCATTGATATTTCTGTTTATTCATGGGAGTTTACCAGAGAAAATGTATGTCTGCGTACTCTCCTAGACATGCTGTTAACCGCAGCTACAGATTCAGCAATAGGAATAAAATAGGACCTATTGTCATGGCGCAGATGGTTGGCCTGTTTAATCAGATTGGAAATTCTAGCATAAAATTACGATTTCTAACTGTAGTGATCTTCTTGCAGATATGGATATCGAGTTGAGAAACTTTCCGCATCATGTTTTTCCTTGTCAGAAGAGAGGTCACGAAGCATTGCCATCACTTTAACATCATTTTGGAATTCTTTTGTTAGAAATTTTAAATCTCTTTCCAGAGGGAATGATTGGGTGCTCTTGCTTAAGGTAACAGTCTGTTTCTCTTTTATTGTTTTATTTATTTAATAAACAAACGTTTGGCCTACTATCATACAATGCCCTTGTTATGAAAAGATAGAGATATGTCAGTACAAGGTTGCCCAAAATATTACATGGGTGCCCAAAGTTGAGTTTGCATTCCTGTGCCAGCATGCAACTTTGTTCTTTTTTTTCTTTTTAAATGATTCCATGGTTCTTTCTAAGAAAAGAGAGAAGGGGTGTATAAGAATGGGAGATGAGGTACCCCGGAACGAAATCTAAGCCGATGAAGATAGTAGGAAACTTAATAAGCTTTCAACTTAAAGCACTCCACAAGCTTTAAAAAGTTAGAAAGAAACCTGCATAATTCTTCAAAAATTATATATATATGTCGATGGAATCAACATCTTTCAGTGAGAAAAAAGTGAGACTATAAGGGCATACCAAAAAAAAACCCATAAAACTCCTAATTGCAGAAAAGTTCCTAACTAAGATTTACCTAGAAAATAATTACAAAAAAGTCTTAAATTACCCAAAAGCTTAAGTTTATGGGTGAAAGTAGATTTAATGGTATGCTATCTTAACACTCTTCACTTGTGGACTCGAAATCTGTAGAAGACTCAATAAGTGGAAATCAATACTAAATTGGGGGAGGAAATAACATTGTTGGAGTTTGAACATAGGATATTCTAGACACTTGCTTTTATACTAACTTAAATCACCGATTGAACCAAAAGCTCTAGCTTACTACTTTACAGATGAAGGTAAATTTTATATTATATCATCTAAGAAAAAGTTCTGATATAGAAGCTCAAAGTGAAACATGAAATCTTACTTAGAACCAAATACTATATTGTCCTCTCTCTCTCTCTAGAGAACCCATAACACAACACGCACCCCAGCAAACGACAATAAATGACCCTTCTCCCTGATAGGTAGGTGGAGGAGGAACTAATCAATCATCGCACAAATACTCCTTTGACCAACTAACTCAACATCAAACTCCTATAGGAAATAGATCCACATATGGGAACCACAAAGAAGGTGATCGAGGTTTTCCTCCACCTTTCGACAAAGAATACATCACAAAGGACCCATTACCGTAGTCATCTTCTGAACAACCCTATCCAAGGTATTCAGCCAAACTAACAAAACTTGTAAGATAAACAACTTGATTTTTTTGGAAATCTTAATCCTTCAAACCACATCAAAGACCAACTCATTAATTGGAAGAGATCCAACAATAGCCTGAAGAAAGACTTACAAGAAAAGCCCTTGATGGGTTTAGGACTCAAGACAAGAACATCTCGTCTTTCAGCCCTAACATTGCACTTTTCTAACAAAGAAAGAATAGGGGCAACCTTTGTTGTTTTCTTATTGGATAAATGACAATGGAAACCAAAAGAAAAGGGCACATAGCCCCCCAACCAAACCAAAAAATCAGAAATCATACGATTTTTAAAGAAAGACAAATGATATAGATGAGCAAATGTGAACAATGAGGACTATCTCCTACTCATTGATCTACCCAAAAATACGCATCCTTACCCCCACAATGCAACAGACCCAGGTCGAATTTAATGTTAGTGTACTAAGTAAAGAGCAATCACACCGTTTATTTTTTCTTTGGATTGCTTACAATCTGAGGCATGGATGTAATGCCTGATTTAACACCCTAAATTAGTGTAGAATCCAATAGTAATTGGTATTTGATCAAAGTACGAGATATTCCTATACTTCGACATAAGCTTTGTGTTTGATTAACTTAGATCTTGTTTTGATCAATTAACAGGTTGCTGTATCACTGTTGGCTCTCAGCTTTATTGGAGTGGTTTCTCTTCAAACTTTATATAAGACAGGTTGCTCCCTCTTCCTCCCTACTCCCTCTCCATCGTGTCATGAACTCGGACGATCTCAATAACTGAAATTGTAGGTTGATACCAGAGATAAAGTCTCGAATTGTAAATATTGCCTCTCAAGATTCACTATGTTCTTATCACTGCTACTAATGTATTTCTTGAATTTATTATTAATTTCCTGAAAGAATTATCTGAGTCCCAGAAGTTCATAGGTGTGTTTGAGGAACATCATTTTTTATTGTACGTTGGAGTCCTACGGATGAAAGTTTTTTGATATGACACACCAAGTAGAAAGCTAAACTGTATTTGTTATATATTGCAGTGGTTCTATTTGCCTTCACGGGTTTCTTCATATACGAGAAAAAGGAGCGAGAGATTGATTCTTTGTGTCAAAGATTTCAAGTTTGGACGCGAAGCAAATGCAGTGTGGTCGGAAAAGTTTCAACATCAAAAGAATAA

mRNA sequence

ATGGGGAGCGAGGAGAAATGGTGCGTGGTCACGGGAGGGAGAGGCTTCGCCGCACGACATCTCGTTGAAATGCTGATCAAATACAACATGTTTTCCGTTCGAATCGTTGACTTGGGCTCTTCCATTGAATTGGAAGCAAGCGAGGAGCAAGGGACTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAGTACGTTTCAGCGGATCTTCGGGACAAAGCTCAAGTGCTCAAAGCGTTCGAAGGAGTGGAGGTTGTTTTCCACATGGCTGCTCCGAATTCATCTATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGAGCTAAGAATGTAATTGATTCTTGTGTCGAGCAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTATTTGATGGGATAAATGCACTTATCAATACGGATGAGTCAATGCCCTACGCATCCAAGGCAAATGGCACAAATGGACTTCTAACGTGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTCGCCAATGCAAGGGCAGGGAAATCCAAGTTCATTATTGGTGATGGCAATAACACCTATGATTTTACTTATGTGGAAAACGTGGGGCATGCCCATGTATGTGCTGAACGAGCTCTAGCTGCAGGAGGTGTAATTTCAGAAAGAGCTGCAGGGCAGGCTTATTTCATAACCAACATGGAACCCATCAAGTTCTGGGAGTTTGTCTCTCTTATTCTGGATGGTCTTGGCTATGAAAGGCCAAGAATAAAGATCCCTGCCTGCATAGTGATGCCAATTGCACATATGGTGAACTGGACTTACAAGCTTTTAGGTCCTTATGGGATGCCTGTCCCACAGTTTACACCTTCCCGTATAAGACTTCTCTCTTGTTGTAGAACTTTTAATTCCTCAAAAGCCAAGGATCGACTTTTCTACACCCCCATTGTTTCACTTCAGGAGGGTATACAAAGGACAATTGAAGCCTACTCCCATTTAAAGGCTGAGCATGAACCTAAAAGAGAAGGGCCATCCAAAGCCTCAATATACCTCAGGGGAGGAACAGCTGCTGACATTTTACTCTGGAAGGATAAAAGGAAGACATTGATAGCATTGATAGTTTTGATGGCTATTTATTATAACTTCATTGCTCCCGAGTGTACCATGATCACTGCACTGTCAAAGCTTCTTATGGTGGCATTGATATTTCTGTTTATTCATGGGAGTTTACCAGAGAAAATATATGGATATCGAGTTGAGAAACTTTCCGCATCATGTTTTTCCTTGTCAGAAGAGAGGTCACGAAGCATTGCCATCACTTTAACATCATTTTGGAATTCTTTTGTTAGAAATTTTAAATCTCTTTCCAGAGGGAATGATTGGGTGCTCTTGCTTAAGGTTGCTGTATCACTGTTGGCTCTCAGCTTTATTGGAGTGGTTTCTCTTCAAACTTTATATAAGACAGTGGTTCTATTTGCCTTCACGGGTTTCTTCATATACGAGAAAAAGGAGCGAGAGATTGATTCTTTGTGTCAAAGATTTCAAGTTTGGACGCGAAGCAAATGCAGTGTGGTCGGAAAAGTTTCAACATCAAAAGAATAA

Coding sequence (CDS)

ATGGGGAGCGAGGAGAAATGGTGCGTGGTCACGGGAGGGAGAGGCTTCGCCGCACGACATCTCGTTGAAATGCTGATCAAATACAACATGTTTTCCGTTCGAATCGTTGACTTGGGCTCTTCCATTGAATTGGAAGCAAGCGAGGAGCAAGGGACTCTCGGAGAGGCATTGCGTTCTGGTCGTGCTCAGTACGTTTCAGCGGATCTTCGGGACAAAGCTCAAGTGCTCAAAGCGTTCGAAGGAGTGGAGGTTGTTTTCCACATGGCTGCTCCGAATTCATCTATTAACAATTACAAACTTCATTATTCGGTCAACGTTGAAGGAGCTAAGAATGTAATTGATTCTTGTGTCGAGCAAAATGTAAAGCGGCTAATATATACTAGTTCGGCCAGTGTAGTATTTGATGGGATAAATGCACTTATCAATACGGATGAGTCAATGCCCTACGCATCCAAGGCAAATGGCACAAATGGACTTCTAACGTGCAGCCTAAGGCCCAGTGGCATCTTTGGCCCTGGTGATAGGTTACTGGTTCCATCCCTAGTCGCCAATGCAAGGGCAGGGAAATCCAAGTTCATTATTGGTGATGGCAATAACACCTATGATTTTACTTATGTGGAAAACGTGGGGCATGCCCATGTATGTGCTGAACGAGCTCTAGCTGCAGGAGGTGTAATTTCAGAAAGAGCTGCAGGGCAGGCTTATTTCATAACCAACATGGAACCCATCAAGTTCTGGGAGTTTGTCTCTCTTATTCTGGATGGTCTTGGCTATGAAAGGCCAAGAATAAAGATCCCTGCCTGCATAGTGATGCCAATTGCACATATGGTGAACTGGACTTACAAGCTTTTAGGTCCTTATGGGATGCCTGTCCCACAGTTTACACCTTCCCGTATAAGACTTCTCTCTTGTTGTAGAACTTTTAATTCCTCAAAAGCCAAGGATCGACTTTTCTACACCCCCATTGTTTCACTTCAGGAGGGTATACAAAGGACAATTGAAGCCTACTCCCATTTAAAGGCTGAGCATGAACCTAAAAGAGAAGGGCCATCCAAAGCCTCAATATACCTCAGGGGAGGAACAGCTGCTGACATTTTACTCTGGAAGGATAAAAGGAAGACATTGATAGCATTGATAGTTTTGATGGCTATTTATTATAACTTCATTGCTCCCGAGTGTACCATGATCACTGCACTGTCAAAGCTTCTTATGGTGGCATTGATATTTCTGTTTATTCATGGGAGTTTACCAGAGAAAATATATGGATATCGAGTTGAGAAACTTTCCGCATCATGTTTTTCCTTGTCAGAAGAGAGGTCACGAAGCATTGCCATCACTTTAACATCATTTTGGAATTCTTTTGTTAGAAATTTTAAATCTCTTTCCAGAGGGAATGATTGGGTGCTCTTGCTTAAGGTTGCTGTATCACTGTTGGCTCTCAGCTTTATTGGAGTGGTTTCTCTTCAAACTTTATATAAGACAGTGGTTCTATTTGCCTTCACGGGTTTCTTCATATACGAGAAAAAGGAGCGAGAGATTGATTCTTTGTGTCAAAGATTTCAAGTTTGGACGCGAAGCAAATGCAGTGTGGTCGGAAAAGTTTCAACATCAAAAGAATAA

Protein sequence

MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRLIYTSSASVVFDGINALINTDESMPYASKANGTNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWTRSKCSVVGKVSTSKE
Homology
BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match: Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)

HSP 1 Score: 694.9 bits (1792), Expect = 7.2e-199
Identity = 352/554 (63.54%), Postives = 428/554 (77.26%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L+  E  G L E LRSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 65  VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           +SADLRDK+QV+KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPY------------------ASKANGTNGLLTCSLRP 184
           IYTSS SVVFDG++ ++N  ESM Y                    KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
           S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI+RTI+++SHL A  + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368

Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVA 424
           REGPSKAS  L GG  AD LLWKD ++TLIA+ +L++IYYNF+A   T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428

Query: 425 LIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGND 484
            +FLF+HG LPEKI+GY VEK+ AS F LS++ S  +++++ S WN+ V+  +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488

Query: 485 WVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-R 540
           W    KV   LLALS  G +SL +++   +  AF  F +YEKKE+EIDS+   F+ +  +
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACK 548

BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match: Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 279/445 (62.70%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY                    KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIA---PECTMITALSKLL 424
            E               + + W  K++TLIA+++L+ +Y+NF+A       +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428

Query: 425 MVALIFLFIHGSLPEKIYGYRVEKL 429
           +V+ IF+FI+G LPEK+  +  +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match: A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)

HSP 1 Score: 374.4 bits (960), Expect = 2.2e-102
Identity = 221/537 (41.15%), Postives = 314/537 (58.47%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S  K CVV GGRGF  R LV  L++   ++VR+ D G ++ L+  E    L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69

Query: 63  QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI +C E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTCSL 182
           +LIY S+A VVFDG   + + DES+    K                  AN  +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL +  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  +V+ +  ++ WT++  
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEH 362
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++GI  T++ +S    E 
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369

Query: 363 EPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLL 422
                  S A   L  G  ADILLW++++KT ++ +VL   YY F     T  ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429

Query: 423 MVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSR 482
            +  + L+    +P KI+G++V K+    F +SE   R ++  +   WN  VR+FKSLS 
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489

Query: 483 GNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 521
           G DW+   K+A SL  L  I   SL     TV+ F+FTGFFIYE+ E E+  L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537

BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match: Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 131/359 (36.49%), Postives = 193/359 (53.76%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S+ K C V GG GF  +H+VE L++   ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD---------------NPRV 83

Query: 63  QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
           Q+   DL ++  +  A +GV  VFH A+P    NN +L Y VN  G K VI++C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQ 143

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKA-------------------NGTNGLLTCS 182
           +LI TSSASVVF+G++ + N  E +PYA K                    +     LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
           +RP GIFGP D  LVP L+  AR GK KF+IG+G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLSQD 263

Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP  +   +A +++    +
Sbjct: 264 AAL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMV 323

Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 343
           + P     P FTP R+ L      ++  KAK    Y P+V++ E ++RT++++ HL+ +
Sbjct: 324 VSPLIQIQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361

BLAST of IVF0004486 vs. ExPASy Swiss-Prot
Match: Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 131/359 (36.49%), Postives = 194/359 (54.04%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S+ K C V GG GF  +H+VE L+    ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD---------------NPRV 83

Query: 63  QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
           Q+   DL ++  +  A +GV  VFH A+P S+ NN +L Y VN  G K VI++C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQ 143

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKA-------------------NGTNGLLTCS 182
           +LI TSSASVVF+G++ + N  E +PYA K                    +     LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
           +RP GIFGP D  LVP L+  AR GK KF+IG+G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSRD 263

Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP  +   +A +++    +
Sbjct: 264 AGL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMV 323

Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 343
           L P       FTP R+ L      ++  KAK  + Y P+V++ + ++RT++++ HL+ +
Sbjct: 324 LSPLIQIQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361

BLAST of IVF0004486 vs. ExPASy TrEMBL
Match: A0A1S3BDP7 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.3e-303
Identity = 539/557 (96.77%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYAS                  KANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
           RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of IVF0004486 vs. ExPASy TrEMBL
Match: A0A0A0KDI8 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G361340 PE=4 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.7e-296
Identity = 526/557 (94.43%), Postives = 533/557 (95.69%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYAS                  KANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+YLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
           RGNDW+LLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of IVF0004486 vs. ExPASy TrEMBL
Match: A0A6J1KG57 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 487/556 (87.59%), Postives = 516/556 (92.81%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYAS                  KANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHGSLPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
           RGNDWV LLKV+VSLLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542

BLAST of IVF0004486 vs. ExPASy TrEMBL
Match: A0A6J1G542 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.0e-277
Identity = 486/557 (87.25%), Postives = 517/557 (92.82%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGA+NVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGARNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYAS                  KANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHG LPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGILPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540
           RGNDWV LLKV+V+LLALSFIG+VSLQTLYKTVVLFAFT FFIYEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVALLALSFIGLVSLQTLYKTVVLFAFTVFFIYEKKEQEIDSLCQKFQF 542

BLAST of IVF0004486 vs. ExPASy TrEMBL
Match: A0A1S3BDV0 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.0e-277
Identity = 494/512 (96.48%), Postives = 494/512 (96.48%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYAS                  KANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 495
           RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 512

BLAST of IVF0004486 vs. NCBI nr
Match: XP_008445550.1 (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 539/557 (96.77%), Postives = 539/557 (96.77%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASK                  ANGTNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
           RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of IVF0004486 vs. NCBI nr
Match: XP_011657360.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis sativus] >KGN47573.1 hypothetical protein Csa_018962 [Cucumis sativus])

HSP 1 Score: 1029 bits (2661), Expect = 0.0
Identity = 526/557 (94.43%), Postives = 533/557 (95.69%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASK                  ANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+YLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
           RGNDW+LLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of IVF0004486 vs. NCBI nr
Match: XP_038884192.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884193.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884195.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida])

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 505/557 (90.66%), Postives = 526/557 (94.43%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGILGEALRSG 60

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQVLKAFEG EVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGAEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
           VKRLIYTSSASVVFDGIN L N DESMPYASK                  ANG++GLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINGLTNADESMPYASKHNDFYSATKAEGEALVLKANGSSGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNAYDFTYVENVAHAHVCAERALAS 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+ISE+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGIISEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTF+SSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFDSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           HE K+EGPSKAS YLRGGTAADILLWKDKRKTL+AL+VL+AIYYNFIAPE TMITALSKL
Sbjct: 361 HEHKKEGPSKASAYLRGGTAADILLWKDKRKTLVALLVLVAIYYNFIAPESTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           L+VALIFLFIHGSLPEKIYGYRVEK+S+SCFSLSEERSRSIAIT+TSFWNSF+R+FKSLS
Sbjct: 421 LLVALIFLFIHGSLPEKIYGYRVEKISSSCFSLSEERSRSIAITVTSFWNSFIRHFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 539
           RGNDWVLLLKVAVSLLALSFIGV+SLQTLYKTVVLFAFTGFF+YEKKE+EIDSLCQRFQ 
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVISLQTLYKTVVLFAFTGFFLYEKKEQEIDSLCQRFQA 540

BLAST of IVF0004486 vs. NCBI nr
Match: XP_022999664.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999665.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999667.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999668.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 487/556 (87.59%), Postives = 516/556 (92.81%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYASK                  ANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHGSLPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 538
           RGNDWV LLKV+VSLLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542

BLAST of IVF0004486 vs. NCBI nr
Match: XP_023546590.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546591.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546592.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo] >XP_023546593.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 486/556 (87.41%), Postives = 516/556 (92.81%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62

Query: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RAQYVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYASK                  ANG NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI+RTIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKASIYLRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHG LPEK++GY+VEK+S+SCFSLSEERSRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGILPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 538
           RGNDWV LLKV+V+LLALSFIG+VSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVALLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542

BLAST of IVF0004486 vs. TAIR 10
Match: AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 694.9 bits (1792), Expect = 5.1e-200
Identity = 352/554 (63.54%), Postives = 428/554 (77.26%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L+  E  G L E LRSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 65  VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           +SADLRDK+QV+KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPY------------------ASKANGTNGLLTCSLRP 184
           IYTSS SVVFDG++ ++N  ESM Y                    KANG NGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
           S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI+RTI+++SHL A  + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368

Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVA 424
           REGPSKAS  L GG  AD LLWKD ++TLIA+ +L++IYYNF+A   T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428

Query: 425 LIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGND 484
            +FLF+HG LPEKI+GY VEK+ AS F LS++ S  +++++ S WN+ V+  +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488

Query: 485 WVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-R 540
           W    KV   LLALS  G +SL +++   +  AF  F +YEKKE+EIDS+   F+ +  +
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACK 548

BLAST of IVF0004486 vs. TAIR 10
Match: AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-163
Identity = 293/471 (62.21%), Postives = 360/471 (76.43%), Query Frame = 0

Query: 88  MAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRLIYTSSASVVFDGINALINTDESM 147
           MAAP+SSINN++L YSVNV+G +NVID+CV+  VKRLIYTSS SVVFDG++ ++N  ESM
Sbjct: 1   MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESM 60

Query: 148 PY------------------ASKANGTNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGK 207
            Y                    KANG NGLLTC +RPS IFGPGDRLLVPSLVA ARAGK
Sbjct: 61  AYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGK 120

Query: 208 SKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVISERAAGQAYFITNMEPIKFWEFV 267
           SKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +S +AAGQAYFITNMEPIKFWEF+
Sbjct: 121 SKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFM 180

Query: 268 SLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGPYGMPVPQFTPSRIRLLSCCRTFN 327
           S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGPYGM VPQ TPSR+RLLSC RTF+
Sbjct: 181 SQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFD 240

Query: 328 SSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPKREGPSKASIYLRGGTAADILLWK 387
           S+KAKDRL Y P+V LQEGI+RTI+++SHL A  + KREGPSKAS  L GG  AD LLWK
Sbjct: 241 STKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWK 300

Query: 388 DKRKTLIALIVLMAIYYNFIAPECTMITALSKLLMVALIFLFIHGSLPEKIYGYRVEKLS 447
           D ++TLIA+ +L++IYYNF+A   T++TALSK L+VA +FLF+HG LPEKI+GY VEK+ 
Sbjct: 301 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 360

Query: 448 ASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSRGNDWVLLLKVAVSLLALSFIGVVSLQ 507
           AS F LS++ S  +++++ S WN+ V+  +SL +GNDW    KV   LLALS  G +SL 
Sbjct: 361 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 420

Query: 508 TLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQVWT-RSKCSVVGKVSTSKE 540
           +++   +  AF  F +YEKKE+EIDS+   F+ +  + K  V  K+  SK+
Sbjct: 421 SIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSDVYEKLFGSKK 471

BLAST of IVF0004486 vs. TAIR 10
Match: AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 553.5 bits (1425), Expect = 1.8e-157
Identity = 279/445 (62.70%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY                    KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 364
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

Query: 365 REGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIA---PECTMITALSKLL 424
            E               + + W  K++TLIA+++L+ +Y+NF+A       +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428

Query: 425 MVALIFLFIHGSLPEKIYGYRVEKL 429
           +V+ IF+FI+G LPEK+  +  +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of IVF0004486 vs. TAIR 10
Match: AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 252/366 (68.85%), Postives = 296/366 (80.87%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAQY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR QY
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K QV+K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYAS------------------KANGTNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY                    KANG +GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEHEPK 353
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI+RTI+++SHLKA+++PK
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

BLAST of IVF0004486 vs. TAIR 10
Match: AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )

HSP 1 Score: 374.4 bits (960), Expect = 1.5e-103
Identity = 221/537 (41.15%), Postives = 314/537 (58.47%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S  K CVV GGRGF  R LV  L++   ++VR+ D G ++ L+  E    L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69

Query: 63  QYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI +C E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASK------------------ANGTNGLLTCSL 182
           +LIY S+A VVFDG   + + DES+    K                  AN  +GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL +  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  +V+ +  ++ WT++  
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAEH 362
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++GI  T++ +S    E 
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369

Query: 363 EPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKLL 422
                  S A   L  G  ADILLW++++KT ++ +VL   YY F     T  ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429

Query: 423 MVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLSR 482
            +  + L+    +P KI+G++V K+    F +SE   R ++  +   WN  VR+FKSLS 
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489

Query: 483 GNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 521
           G DW+   K+A SL  L  I   SL     TV+ F+FTGFFIYE+ E E+  L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67ZE17.2e-19963.543beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... [more]
Q9FX012.6e-15662.703beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... [more]
A9X4U22.2e-10241.153beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... [more]
Q9R1J03.0e-5936.49Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=1... [more]
Q5PPL33.0e-5936.49Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... [more]
Match NameE-valueIdentityDescription
A0A1S3BDP73.3e-30396.77Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1[more]
A0A0A0KDI81.7e-29694.43Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G361340 PE=4 SV=1[more]
A0A6J1KG573.1e-27787.59Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1[more]
A0A6J1G5429.0e-27787.25Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1[more]
A0A1S3BDV09.0e-27796.48Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008445550.10.096.77PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis ... [more]
XP_011657360.10.094.433beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis sativus] >K... [more]
XP_038884192.10.090.663beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_03... [more]
XP_022999664.10.087.593beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022... [more]
XP_023546590.10.087.413beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT2G26260.15.1e-20063.543beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT2G26260.21.7e-16362.213beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT1G47290.21.8e-15762.703beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
AT1G47290.11.2e-14568.853beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
AT2G43420.11.5e-10341.153-beta hydroxysteroid dehydrogenase/isomerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 152..265
e-value: 4.2E-31
score: 108.0
NoneNo IPR availableGENE3D3.40.50.720coord: 2..341
e-value: 7.0E-67
score: 227.9
NoneNo IPR availablePANTHERPTHR10366:SF7253BETA-HYDROXYSTEROID-DEHYDROGENASE/DECARBOXYLASE ISOFORM 1coord: 4..348
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 4..348
IPR003388ReticulonPFAMPF02453Reticuloncoord: 363..518
e-value: 4.9E-34
score: 117.6
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 362..539
score: 15.52329
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 12..132
e-value: 8.9E-10
score: 38.7
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0004486.1IVF0004486.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor