IVF0004201 (gene) Melon (IVF77) v1

Overview
NameIVF0004201
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionMethylenetetrahydrofolate reductase
Locationchr12: 1534035 .. 1535534 (+)
RNA-Seq ExpressionIVF0004201
SyntenyIVF0004201
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGGTACTGTTCCTAACCAAGAGCGTAGAGAAATTTTGTTACAACTGCTAGCGATCAACTAGTAATGTTTAGACTTTTTTGTATCAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGCTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGTAAATACAAGTTAATGCATTTGTACAATTAACTTTTTTGACAGCCCAACTGACCAAGTGTTTGAAAAAGTAGGAAAAGTACTTCAAAACAAAAACTGAACTAGATATTAATAAAAATGAAAGACAATATATTATTTGCCAAGTTACATATTGGAGGGAGTAAATTTAATATCCCCAAGGGAATGAAGCCCACTTCAGTTTCTATTCATATTCTTCTTGAAATCAGAAACCTGTATAGGGCCCATCTGTCAAAAGACAGTTAATGGTCATTTGAAGGGATGAAACACGTGTAGATGGTATGTAAACTTTAACCACGAATATAACATCTTAACAATACAATCATCATGCATGAGTCGCCCTTGTGTAGTGTAGGGGAATTATTATTTTCTTGTTGGAAAATTCAACACTTGGTCTCTACTCTTTTTGTAGTGATCTCATTAGGTTCTAACTTCTGAAATCTGTGCTAAATGGTGGCAAAATGTTTAGTAATCTGACCAATGTTGGGACGATTGATAATGATTTACAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAATTGCTTGATGAGGTTGGTACACTTGCTATATCTTTTCAGTGCTCAATTAATTTTGGTTTAATATTTCCTAAAAAATAACATCCAATGTTATGTGTGATTGAAAGGCTTAATTAGTAAAAAGATTAAATGTTGTTTTATCTACAAGGTTATTAATATATATAATGATAGAAGATGAGAAGGTATGTGGAGGATTATTATTGATGACGAAGCTATGTTATTTGTTTCAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGA

mRNA sequence

ATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGCTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAATTGCTTGATGAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGA

Coding sequence (CDS)

ATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGCTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAATTGCTTGATGAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGA

Protein sequence

MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Homology
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BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match: O80585 (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 366.3 bits (939), Expect = 2.4e-100
Identity = 168/222 (75.68%), Postives = 195/222 (87.84%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSEL+GLQ ETR+I+EQL K+
Sbjct: 372 RPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKV 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N KGFL+INSQP+VN ERSDSP+VGWGGP GYVYQKAY+EFFCS+EKLDA+V+KC+ALPS
Sbjct: 432 NSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           +TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF VWKDEAFE WSR W
Sbjct: 492 ITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 224
           A LYPE D SR LL+E+++SYYLVSLV+NDYIN D+FA+  D
Sbjct: 552 ANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

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BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match: Q9SE60 (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 5.6e-97
Identity = 159/219 (72.60%), Postives = 192/219 (87.67%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSEL+GLQ ET++I+EQLGKI
Sbjct: 372 RPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPETKIINEQLGKI 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N  GFL+INSQP+VN  +SDSP++GWGGPGGYVYQKAY+EFFCS++KLD +V+K +A PS
Sbjct: 432 NSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTLVEKSKAFPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           +TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF VWKDEAFEIWSR W
Sbjct: 492 ITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAI 221
           A LYPE D SRKLL+E+++SYYLVSLVDN+YIN D+F++
Sbjct: 552 ANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSV 590

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BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match: Q75HE6 (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-96
Identity = 161/222 (72.52%), Postives = 192/222 (86.49%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RPR R KKL EEWA+P+KS+ED+ E+FM +C GKL SSPWSEL+GLQ ET++I +QL KI
Sbjct: 372 RPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQPETKIIDDQLVKI 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N KGFL+INSQPAVNGERSDS SVGWGGPGGYVYQKAY+EFFCS+EKLD +++K +A PS
Sbjct: 432 NQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEKLDQLIEKSKAFPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           LTY+AVNK+G   SN+    VNAVTWGVFP KEI+QPTVVD ASF+VWKDEAFEIWS+GW
Sbjct: 492 LTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWKDEAFEIWSKGW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 224
           A L+PEGD SR++LD++Q SY+LVSLVDNDYIN DLFA  ++
Sbjct: 552 ACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKE 593

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BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match: Q9SE94 (Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 5.8e-94
Identity = 155/218 (71.10%), Postives = 188/218 (86.24%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RPR R KKL EEWA+PLKS+ED+ E+F  +C GKL SSPWSEL+GLQ ET++I +QL  I
Sbjct: 372 RPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQPETKIIDDQLVNI 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N KGFL+INSQPAVNGE+SDSP+VGWGGPGGYVYQKAY+EFFC++EKLD +++K +A PS
Sbjct: 432 NQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEKLDQLIEKIKAFPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           LTY+AVNK+G   SN++   VNAVTWGVFP KEIIQPTVVD ASF+VWKDEAFEIW+RGW
Sbjct: 492 LTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWKDEAFEIWTRGW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFA 220
             ++PEGD SR+LL+++Q +YYLVSLVDNDY+  DLFA
Sbjct: 552 GCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFA 589

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BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match: P42898 (Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3)

HSP 1 Score: 203.0 bits (515), Expect = 3.6e-51
Identity = 102/232 (43.97%), Postives = 150/232 (64.66%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLG-------KLRSSPWSELEGLQAETRM 60
           ++ ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ E L AET +
Sbjct: 408 LKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPLAAETSL 467

Query: 61  ISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIV 120
           + E+L ++N +G L+INSQP +NG+ S  P VGWG  GGYV+QKAY+EFF SRE  +A++
Sbjct: 468 LKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALL 527

Query: 121 DKCRALP-SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDE 180
              +     + Y  VN +G  ++N  +   NAVTWG+FP +EIIQPTVVDP SF+ WKDE
Sbjct: 528 QVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDE 587

Query: 181 AFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDY-INSDLFAILED 224
           AF +W   W  LY E   SR ++  I  +Y+LV+LVDND+ +++ L+ ++ED
Sbjct: 588 AFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVED 638

[Close]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match: A0A5A7V2U8 (Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00900 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.4e-127
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 373 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 432

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 433 INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 492

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 493 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 552

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 225
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 553 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

[Close]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match: A0A5D3BFH5 (Methylenetetrahydrofolate reductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001820 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.4e-127
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 108 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 167

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 168 INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 227

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 228 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 287

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 225
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 288 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 331

[Close]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match: A0A1S3CP87 (methylenetetrahydrofolate reductase 2-like OS=Cucumis melo OX=3656 GN=LOC103503268 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.4e-127
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 13  MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 72

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 73  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 132

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 133 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 192

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 225
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 193 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 236

[Close]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match: A0A0A0LIC6 (Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.6e-126
Identity = 221/224 (98.66%), Postives = 222/224 (99.11%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 415 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 474

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSP VGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 475 INMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 534

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF+VWKDEAFEIWSRG
Sbjct: 535 SLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIVWKDEAFEIWSRG 594

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 225
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 595 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

[Close]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match: A0A5A7T632 (Methylenetetrahydrofolate reductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G002820 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.6e-121
Identity = 218/224 (97.32%), Postives = 218/224 (97.32%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 129 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 188

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSV     GGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 189 INMKGFLSINSQPAVNGERSDSPSV-----GGYVYQKAYIEFFCSREKLDAIVDKCRALP 248

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTW VFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 249 SLTYMAVNKEGIWVSNVTQKDVNAVTWSVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 308

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 225
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 309 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 347

[Close]
BLAST of IVF0004201 vs. NCBI nr
Match: XP_008465630.1 (PREDICTED: methylenetetrahydrofolate reductase 2-like, partial [Cucumis melo])

HSP 1 Score: 458 bits (1179), Expect = 1.19e-162
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 13  MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 72

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 73  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 132

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 133 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 192

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 224
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 193 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 236

[Close]
BLAST of IVF0004201 vs. NCBI nr
Match: TYJ97371.1 (methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 458 bits (1179), Expect = 3.67e-161
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 108 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 167

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 168 INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 227

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 228 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 287

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 224
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 288 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 331

[Close]
BLAST of IVF0004201 vs. NCBI nr
Match: KAA0060205.1 (methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 458 bits (1179), Expect = 2.87e-157
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 373 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 432

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 433 INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 492

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 493 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 552

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 224
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 553 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

[Close]
BLAST of IVF0004201 vs. NCBI nr
Match: XP_004138970.2 (methylenetetrahydrofolate reductase 2 [Cucumis sativus] >KGN61503.1 hypothetical protein Csa_006097 [Cucumis sativus])

HSP 1 Score: 454 bits (1168), Expect = 4.93e-155
Identity = 221/224 (98.66%), Postives = 222/224 (99.11%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 415 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 474

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSP VGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 475 INMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 534

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF+VWKDEAFEIWSRG
Sbjct: 535 SLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIVWKDEAFEIWSRG 594

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 224
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 595 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

[Close]
BLAST of IVF0004201 vs. NCBI nr
Match: KAA0038393.1 (methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 437 bits (1124), Expect = 1.53e-152
Identity = 218/224 (97.32%), Postives = 218/224 (97.32%), Query Frame = 0 

Query: 1   MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 60
           MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK
Sbjct: 129 MRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGK 188

Query: 61  INMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALP 120
           INMKGFLSINSQPAVNGERSDSPSVG     GYVYQKAYIEFFCSREKLDAIVDKCRALP
Sbjct: 189 INMKGFLSINSQPAVNGERSDSPSVG-----GYVYQKAYIEFFCSREKLDAIVDKCRALP 248

Query: 121 SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 180
           SLTYMAVNKEGIWVSNVTQKDVNAVTW VFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG
Sbjct: 249 SLTYMAVNKEGIWVSNVTQKDVNAVTWSVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRG 308

Query: 181 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 224
           WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 309 WAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 347

[Close]
BLAST of IVF0004201 vs. TAIR 10
Match: AT2G44160.1 (methylenetetrahydrofolate reductase 2 )

HSP 1 Score: 366.3 bits (939), Expect = 1.7e-101
Identity = 168/222 (75.68%), Postives = 195/222 (87.84%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSEL+GLQ ETR+I+EQL K+
Sbjct: 372 RPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKV 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N KGFL+INSQP+VN ERSDSP+VGWGGP GYVYQKAY+EFFCS+EKLDA+V+KC+ALPS
Sbjct: 432 NSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           +TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF VWKDEAFE WSR W
Sbjct: 492 ITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 224
           A LYPE D SR LL+E+++SYYLVSLV+NDYIN D+FA+  D
Sbjct: 552 ANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

[Close]
BLAST of IVF0004201 vs. TAIR 10
Match: AT3G59970.3 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 355.1 bits (910), Expect = 4.0e-98
Identity = 159/219 (72.60%), Postives = 192/219 (87.67%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKI 61
           RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSEL+GLQ ET++I+EQLGKI
Sbjct: 372 RPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPETKIINEQLGKI 431

Query: 62  NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPS 121
           N  GFL+INSQP+VN  +SDSP++GWGGPGGYVYQKAY+EFFCS++KLD +V+K +A PS
Sbjct: 432 NSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTLVEKSKAFPS 491

Query: 122 LTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGW 181
           +TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF VWKDEAFEIWSR W
Sbjct: 492 ITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSW 551

Query: 182 AALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAI 221
           A LYPE D SRKLL+E+++SYYLVSLVDN+YIN D+F++
Sbjct: 552 ANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSV 590

[Close]
BLAST of IVF0004201 vs. TAIR 10
Match: AT3G59970.2 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 3.4e-04
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYEK 28
           RP+ RDKKL +EW +PLKSIEDV E+
Sbjct: 372 RPKGRDKKLQQEWVVPLKSIEDVQER 397

[Close]
BLAST of IVF0004201 vs. TAIR 10
Match: AT3G59970.1 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 42.4 bits (98), Expect = 5.8e-04
Identity = 18/25 (72.00%), Postives = 21/25 (84.00%), Query Frame = 0 

Query: 2   RPRARDKKLLEEWALPLKSIEDVYE 27
           RP+ RDKKL +EW +PLKSIEDV E
Sbjct: 372 RPKGRDKKLQQEWVVPLKSIEDVQE 396

The following BLAST results are available for this feature:
BLAST of IVF0004201 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O805852.4e-10075.68Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 ... [more]
Q9SE605.6e-9772.60Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 ... [more]
Q75HE62.1e-9672.52Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=... [more]
Q9SE945.8e-9471.10Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1[more]
P428983.6e-5143.97Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3[more]
BLAST of IVF0004201 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7V2U82.4e-127100.00Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A5D3BFH52.4e-127100.00Methylenetetrahydrofolate reductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CP872.4e-127100.00methylenetetrahydrofolate reductase 2-like OS=Cucumis melo OX=3656 GN=LOC1035032... [more]
A0A0A0LIC64.6e-12698.66Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 P... [more]
A0A5A7T6327.6e-12197.32Methylenetetrahydrofolate reductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
BLAST of IVF0004201 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008465630.11.19e-162100.00PREDICTED: methylenetetrahydrofolate reductase 2-like, partial [Cucumis melo][more]
TYJ97371.13.67e-161100.00methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa][more]
KAA0060205.12.87e-157100.00methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 met... [more]
XP_004138970.24.93e-15598.66methylenetetrahydrofolate reductase 2 [Cucumis sativus] >KGN61503.1 hypothetical... [more]
KAA0038393.11.53e-15297.32methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa][more]
BLAST of IVF0004201 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44160.11.7e-10175.68methylenetetrahydrofolate reductase 2 [more]
AT3G59970.34.0e-9872.60methylenetetrahydrofolate reductase 1 [more]
AT3G59970.23.4e-0469.23methylenetetrahydrofolate reductase 1 [more]
AT3G59970.15.8e-0472.00methylenetetrahydrofolate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR45754:SF5METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 1..223
NoneNo IPR availablePANTHERPTHR45754METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 1..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0004201.1IVF0004201.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005829 cytosol
molecular_function GO:0071949 FAD binding
molecular_function GO:0106312 methylenetetrahydrofolate reductase NADH activity
molecular_function GO:0106313 methylenetetrahydrofolate reductase NADPH activity