IVF0004190 (gene) Melon (IVF77) v1

Overview
NameIVF0004190
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionaspartic proteinase Asp1-like
Locationchr11: 79032 .. 82093 (+)
RNA-Seq ExpressionIVF0004190
SyntenyIVF0004190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAAATGGGTATTGGTGGTACTGGCTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTCTTTAAGGATAAGCCATGGGAGAGAAAGAGGCCAATTCTGTCGGTTCCGACAGCATCTTCTTCGTTTGCTTCATCCTCCATTGTGTTGCCTCTTCAAGGGAACGTGTATCCAAATGGGTAAGCTATAACTCTGCTCAAAGATACGACGGTGTCCAGCTTTTGTTTTTCCCATTTCGATTTTACTCTACTGTTATACAGTACGAAGTCCAATGTGAATATGATGACTCCTAATATACTTTTCACATTGATGTATGATAATCCCAGTTGTCCTTCCTCATTCTTGATCTTATTGTATCTTCGCTCGCGTGTTAAAATTGAAGAAAAGATGATTGCGAGACTGTGCTCTCTCATGTTTGCTTCTCTCCTGTGCTATCCATTCCTGTGCAATTGCTTTTGAGGAGTGTATTTATGAGTGATTTTTTCTCAACTTTGTAGCAAAATACAATATCCCCAAATCAAATACTTCTAAAAATTTCAAACCCACTTTACTGAATGTTTTTATTTACGAACGTCGCAGGCTAACTTCTTTACTGTCAGATCCTAAATACTGATTTTCATTAATATGTTTGGAGGTCACTATAATCCGTGTCTTTTTTCAGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTTTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGGTAAACTTTTCTTTGTTGCAAATTTGGAGATTCTTGTAAATGCGATGAACTAAACGATGTAAGATAGTTATTAGAATACAGCTTTAGCTTCAGTTAAATTAATTTTGATCACAAGTAACCCAAAATTAGTTAATTTTGATCATAAGTAACCCAAAATTTCATTCTTGAATGGTCTATTTTGTTCTATGTTCCCGTTATATTTCTTCCTGATGTGCTTAGTGCTTTTTTTCCTTCTTTCGCTCAGTCTCTCACGACCTTTAAAAATCATTGCATTTCTTTTGAGGTTGAGAATTAAGGTTGACTGCTTCAAACTCATGGCCTTGTTTCCTTCACAGACACTTCATCCGCTCTATCAACCAAGCAACGATCTAGTCCCTTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGGTAAACTGCTCAACTGCCTCCAGTAATTCATCTCAATTCAGTGTACTGATGAATTCCTAGCTCTCTATATCAGCCTTGCTATTTCCAATTACAATGTCACATGAGAGTCTTGAAGAAGAAACATCATATAATGCTGTCATATCTTATGTTTTGCACTTCCCTTATATTTCAAGGTAATGATAGCGTGAATGTGAAACTAGAGTATATTTTGAATAACAAACGTGTGCATTCAAGTGATGGGTTACTTCAAGAAAACACTTATTTAAGATAGCAACGGGCCTAGAAATTTTGATGGACATAATGATTCTGATTTGTAATTTTTATAGTCATCAATAAAGATTATCTTGGACAAGAGATTGATATTCACTTTGCTAATTTCTCCTCCTTGCAAACTGCAGTTGTCAGCTCATGTGGGTAAATAGCATGCATGTAAAAGCGGTATGTATTTTTAGCTATTTGAATCTGGAGTACTGAGTTAGTTTTCATTTGATTACTCAGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAATCAAGGCATTGTTCGTAATGTCGTTGGTCACTGTTTCAATAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATTTATGATCCCTATCGCTTAGTTTGGACGCCCATGTCACGGGACTACCGTAAACAACTTGCCTTGATAATATATATATATATATATTATTGTAATGATTCATGTTAGCTTCTGTCTTTATAAAACCTGCCCCTTGTGTGATTGTATCAGGAAGCACTACTCCCCTGGGTTTGGAGAACTAATGTTCAATGGAAGAAGTACTGGACTCAGGAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGGTAAATCATCTGTAAAAGAAACTAGACTGCGTGGGAAGATTAAATCTTTCTATCTCATGAAAACTTTGGGTTAATACGCAGTGATTCTTCAATTCATGTTGGCACTGTTTTTGTAGTTGAATAGAGAATTAGCTGGAAAACCGCTAAGAGAAGCCATGGACGATGATACGCTTCCGCTCTGCTGGAGAGAGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATAGAAGGTTACATGATAATATCGGTAAAAGCTCCACGCCTCAACTCCAACTGCTATTCACTTGACATTTCTTTTATTTGGATTTTCAAGTTGAACGAATTCAATTATATAAGATAACTTTGGAATGCAGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGGTACGTTATTTTAATTTATTGCATGCACATGTTACATTATTTTCTCGAAGCCTTCGAAACCATGCAAGAATATTCTTTTATTAGAGAGAGAGAAGAGAAAAAGAATCCACATCTTCTGTATGCTTTTGTTGACAGATATATCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

mRNA sequence

ATGGGCAAATGGGTATTGGTGGTACTGGCTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTCTTTAAGGATAAGCCATGGGAGAGAAAGAGGCCAATTCTGTCGGTTCCGACAGCATCTTCTTCGTTTGCTTCATCCTCCATTGTGTTGCCTCTTCAAGGGAACGTGTATCCAAATGGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTTTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGACACTTCATCCGCTCTATCAACCAAGCAACGATCTAGTCCCTTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAATCAAGGCATTGTTCGTAATGTCGTTGGTCACTGTTTCAATAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATTTATGATCCCTATCGCTTAGTTTGGACGCCCATGAAGCACTACTCCCCTGGGTTTGGAGAACTAATGTTCAATGGAAGAAGTACTGGACTCAGGAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGTTGAATAGAGAATTAGCTGGAAAACCGCTAAGAGAAGCCATGGACGATGATACGCTTCCGCTCTGCTGGAGAGAGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATAGAAGGTTACATGATAATATCGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGATATATCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

Coding sequence (CDS)

ATGGGCAAATGGGTATTGGTGGTACTGGCTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTCTTTAAGGATAAGCCATGGGAGAGAAAGAGGCCAATTCTGTCGGTTCCGACAGCATCTTCTTCGTTTGCTTCATCCTCCATTGTGTTGCCTCTTCAAGGGAACGTGTATCCAAATGGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTTTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGACACTTCATCCGCTCTATCAACCAAGCAACGATCTAGTCCCTTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAATCAAGGCATTGTTCGTAATGTCGTTGGTCACTGTTTCAATAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATTTATGATCCCTATCGCTTAGTTTGGACGCCCATGAAGCACTACTCCCCTGGGTTTGGAGAACTAATGTTCAATGGAAGAAGTACTGGACTCAGGAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGTTGAATAGAGAATTAGCTGGAAAACCGCTAAGAGAAGCCATGGACGATGATACGCTTCCGCTCTGCTGGAGAGAGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATAGAAGGTTACATGATAATATCGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGATATATCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

Protein sequence

MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGIYDPYRLVWTPMKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDRVPKSQVSSW
Homology
BLAST of IVF0004190 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.3e-90
Identity = 181/388 (46.65%), Postives = 251/388 (64.69%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++VL L GNVYP G + +T+ +G P K YFLD DTGS LTWLQCDAPC  C    H L
Sbjct: 21  SSAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVL 80

Query: 111 YQPS-NDLVPCKDPLCMSLHSSM--DHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNL 170
           Y+P+   LV C D LC  L++ +    RC +  QCDY ++Y D  SS+GVLV D F L+ 
Sbjct: 81  YKPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSA 140

Query: 171 TNGDPIRPRLALGCGYDQDPGSSSYH-PMDGILGLGRGAVSIVSQLHNQGIV-RNVVGHC 230
           +NG      +A GCGYDQ   + +   P+D ILGL RG V+++SQL +QG++ ++V+GHC
Sbjct: 141 SNGTN-PTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 231 FNSKGGGYLFFGDGIYDPYRLVWTPM----KHYSPGFGELMF--NGRSTGLRNLFVVFDS 290
            +SKGGG+LFFGD       + WTPM    K+YSPG G L F  N ++     + V+FDS
Sbjct: 201 ISSKGGGFLFFGDAQVPTSGVTWTPMNREHKYYSPGHGTLHFDSNSKAISAAPMAVIFDS 260

Query: 291 GSSYTYFNAQAYQ----VLTSLLNRELAGKPLREAMDDD-TLPLCWRERKPIKSLRDVRK 350
           G++YTYF AQ YQ    V+ S LN E   K L E  + D  L +CW+ +  I ++ +V+K
Sbjct: 261 GATYTYFAAQPYQATLSVVKSTLNSEC--KFLTEVTEKDRALTVCWKGKDKIVTIDEVKK 320

Query: 351 YFKPLALSFSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGT--DVGLENSNIIGDISM 410
            F+ L+L F+ G + KA  EIP E Y+IIS  G+VCLGIL+G+   + L  +N+IG I+M
Sbjct: 321 CFRSLSLEFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITM 380

Query: 411 QDKMVVYNNEKQAIGWATANCDRVPKSQ 421
            D+MV+Y++E+  +GW    CDR+P+S+
Sbjct: 381 LDQMVIYDSERSLLGWVNYQCDRIPRSE 403

BLAST of IVF0004190 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-85
Identity = 171/385 (44.42%), Postives = 244/385 (63.38%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++VL L GNVYP G + VT+ +G P KPYFLD DTGS LTWLQCD PC  C +  H L
Sbjct: 21  SSAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGL 80

Query: 111 YQPS-NDLVPCKDPLCMSLHSSM--DHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNL 170
           Y+P     V C +  C  L++ +    +C   +QC Y ++Y  GGSS+GVL+ D F L  
Sbjct: 81  YKPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYV-GGSSIGVLIVDSFSLPA 140

Query: 171 TNGDPIRPRLALGCGYDQDPGSSSY-HPMDGILGLGRGAVSIVSQLHNQGIV-RNVVGHC 230
           +NG      +A GCGY+Q   + +   P++GILGLGRG V+++SQL +QG++ ++V+GHC
Sbjct: 141 SNGTN-PTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 231 FNSKGGGYLFFGDGIYDPYRLVWTPM----KHYSPGFGELMFNGRSTGL--RNLFVVFDS 290
            +SKG G+LFFGD       + W+PM    KHYSP  G L FN  S  +    + V+FDS
Sbjct: 201 ISSKGKGFLFFGDAKVPTSGVTWSPMNREHKHYSPRQGTLQFNSNSKPISAAPMEVIFDS 260

Query: 291 GSSYTYFNAQAYQVLTSLLNRELAG--KPLREAMDDD-TLPLCWRERKPIKSLRDVRKYF 350
           G++YTYF  Q Y    S++   L+   K L E  + D  L +CW+ +  I+++ +V+K F
Sbjct: 261 GATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKCF 320

Query: 351 KPLALSFSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGT--DVGLENSNIIGDISMQD 410
           + L+L F+ G + KA  EIP E Y+IIS  G+VCLGIL+G+     L  +N+IG I+M D
Sbjct: 321 RSLSLKFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLD 380

Query: 411 KMVVYNNEKQAIGWATANCDRVPKS 420
           +MV+Y++E+  +GW    CDR+P+S
Sbjct: 381 QMVIYDSERSLLGWVNYQCDRIPRS 402

BLAST of IVF0004190 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.0e-80
Identity = 170/397 (42.82%), Postives = 237/397 (59.70%), Query Frame = 0

Query: 44  TASSSFASSSIVLPLQGNVYPNGFYNVTLYVGQPP--KPYFLDPDTGSDLTWLQCDAPCQ 103
           T++ S  SS+ + P+ GNVYP+G Y   + VG+P   + Y LD DTGS+LTW+QCDAPC 
Sbjct: 179 TSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCT 238

Query: 104 QCTETLHPLYQPSND-LVPCKDPLCMSL-HSSMDHRCENPDQCDYEVEYADGGSSLGVLV 163
            C +  + LY+P  D LV   +  C+ +  + +   CEN  QCDYE+EYAD   S+GVL 
Sbjct: 239 SCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCHQCDYEIEYADHSYSMGVLT 298

Query: 164 RDVFPLNLTNGDPIRPRLALGCGYDQDP-GSSSYHPMDGILGLGRGAVSIVSQLHNQGIV 223
           +D F L L NG      +  GCGYDQ     ++    DGILGL R  +S+ SQL ++GI+
Sbjct: 299 KDKFHLKLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGII 358

Query: 224 RNVVGHCFNS--KGGGYLFFGDGIYDPYRLVWTPMKH-------------YSPGFGELMF 283
            NVVGHC  S   G GY+F G  +   + + W PM H              S G G L  
Sbjct: 359 SNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSL 418

Query: 284 NGRSTGLRNLFVVFDSGSSYTYFNAQAY-QVLTSLLNRELAGKPLREAMDDDTLPLCWRE 343
           +G +  +    V+FD+GSSYTYF  QAY Q++TSL  +E++G  L     D+TLP+CWR 
Sbjct: 419 DGENGRVGK--VLFDTGSSYTYFPNQAYSQLVTSL--QEVSGLELTRDDSDETLPICWRA 478

Query: 344 RK--PIKSLRDVRKYFKPLALSFSSGGR--SKAVFEIPIEGYMIISSMGNVCLGILNGTD 403
           +   P  SL DV+K+F+P+ L   S     S+ +   P E Y+IIS+ GNVCLGIL+G+ 
Sbjct: 479 KTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLLIQP-EDYLIISNKGNVCLGILDGSS 538

Query: 404 VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 416
           V   ++ I+GDISM+  ++VY+N K+ IGW  ++C R
Sbjct: 539 VHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVR 570

BLAST of IVF0004190 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 2.2e-33
Identity = 114/390 (29.23%), Postives = 174/390 (44.62%), Query Frame = 0

Query: 52  SSIVLPLQGN--VYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP 111
           +SI LPL G+  V   G Y   + +G PPK Y +  DTGSD+ W+ C  PC +C    + 
Sbjct: 56  ASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPTKTNL 115

Query: 112 LYQPS---------NDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVR 171
            ++ S         +  V C D  C  +  S    C+    C Y + YAD  +S G  +R
Sbjct: 116 NFRLSLFDMNASSTSKKVGCDDDFCSFI--SQSDSCQPALGCSYHIVYADESTSDGKFIR 175

Query: 172 DVFPLNLTNGD----PIRPRLALGCGYDQDPG-SSSYHPMDGILGLGRGAVSIVSQLHNQ 231
           D+  L    GD    P+   +  GCG DQ     +    +DG++G G+   S++SQL   
Sbjct: 176 DMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAAT 235

Query: 232 GIVRNVVGHCF-NSKGGGYLFFGDGIYDPYRLVWTPM----KHYSPGFGELMFNGRSTGL 291
           G  + V  HC  N KGGG   F  G+ D  ++  TPM     HY+     +  +G S  L
Sbjct: 236 GDAKRVFSHCLDNVKGGG--IFAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDL 295

Query: 292 -----RNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKP 351
                RN   + DSG++  YF    Y    SL+   LA +P++  + ++T        + 
Sbjct: 296 PRSIVRNGGTIVDSGTTLAYFPKVLYD---SLIETILARQPVKLHIVEETF-------QC 355

Query: 352 IKSLRDVRKYFKPLALSFSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSN 411
                +V + F P++  F    +      +    Y+        C G   G     E S 
Sbjct: 356 FSFSTNVDEAFPPVSFEFEDSVK----LTVYPHDYLFTLEEELYCFGWQAGGLTTDERSE 415

Query: 412 II--GDISMQDKMVVYNNEKQAIGWATANC 414
           +I  GD+ + +K+VVY+ + + IGWA  NC
Sbjct: 416 VILLGDLVLSNKLVVYDLDNEVIGWADHNC 426

BLAST of IVF0004190 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-27
Identity = 112/373 (30.03%), Postives = 165/373 (44.24%), Query Frame = 0

Query: 67  FYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP---------LYQP---- 126
           + NVT  VG P   + +  DTGSDL WL CD  C  C   L           +Y P    
Sbjct: 105 YANVT--VGTPSDWFMVALDTGSDLFWLPCD--CTNCVRELKAPGGSSLDLNIYSPNASS 164

Query: 127 SNDLVPCKDPLCMSLHSSMDHRCENPD-QCDYEVEY-ADGGSSLGVLVRDVFPL--NLTN 186
           ++  VPC   LC     +   RC +P+  C Y++ Y ++G SS GVLV DV  L  N  +
Sbjct: 165 TSTKVPCNSTLC-----TRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKS 224

Query: 187 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 246
              I  R+  GCG  Q          +G+ GLG   +S+ S L  +GI  N    CF + 
Sbjct: 225 SKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFGND 284

Query: 247 GGGYLFFGD-GIYDPYRL---VWTPMKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTY 306
           G G + FGD G  D       +  P   Y+    ++   G +TG      VFDSG+S+TY
Sbjct: 285 GAGRISFGDKGSVDQRETPLNIRQPHPTYNITVTKISVGG-NTGDLEFDAVFDSGTSFTY 344

Query: 307 FNAQAYQVLTSLLNRELAGKPLREAMDDDTLPL--CWRERKPIKSLRDVRKYFKPLALSF 366
               AY +++   N     K  R    D  LP   C+       +L   +  F+  A++ 
Sbjct: 345 LTDAAYTLISESFNSLALDK--RYQTTDSELPFEYCY-------ALSPNKDSFQYPAVNL 404

Query: 367 SSGGRSKAVFEIPIEGYMIISSMGNV---CLGILNGTDVGLENSNIIGDISMQDKMVVYN 414
           +  G S      P+   +++  M +    CL I+      +E+ +IIG   M    VV++
Sbjct: 405 TMKGGS----SYPVYHPLVVIPMKDTDVYCLAIMK-----IEDISIIGQNFMTGYRVVFD 449

BLAST of IVF0004190 vs. ExPASy TrEMBL
Match: A0A5D3BS69 (Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00070 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.2e-252
Identity = 424/429 (98.83%), Postives = 424/429 (98.83%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 42  MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 101

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 102 NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 161

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 162 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 221

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 222 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 281

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 282 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 341

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 342 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 401

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 402 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 461

Query: 421 VPKSQVSSW 425
           VPKSQVSSW
Sbjct: 462 VPKSQVSSW 470

BLAST of IVF0004190 vs. ExPASy TrEMBL
Match: A0A1S3CDB2 (aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.2e-252
Identity = 424/429 (98.83%), Postives = 424/429 (98.83%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 425
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of IVF0004190 vs. ExPASy TrEMBL
Match: A0A1S3CDB4 (aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 6.7e-251
Identity = 424/433 (97.92%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 ----CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240
               CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF
Sbjct: 181 CQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240

Query: 241 GDGIYDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDGIYDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300

Query: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360
           QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA
Sbjct: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360

Query: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420

Query: 421 NCDRVPKSQVSSW 425
           NCDRVPKSQVSSW
Sbjct: 421 NCDRVPKSQVSSW 433

BLAST of IVF0004190 vs. ExPASy TrEMBL
Match: A0A6J1DZ15 (aspartic proteinase Asp1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024375 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 377/428 (88.08%), Postives = 404/428 (94.39%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MG  +L +L LMVASM+CLAP SASSFFKDKPWER++PILSV   SSSFASSSIVLPLQG
Sbjct: 1   MGTGLLKILVLMVASMNCLAPSSASSFFKDKPWERRKPILSVSATSSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLY+PS+DLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYRPSDDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSVDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQ PGSS YHPMDGILGLG+GAVSIVSQLHNQGI+RNV+GHCF+S+GGGYLFFGD I
Sbjct: 181 CGYDQIPGSSYYHPMDGILGLGKGAVSIVSQLHNQGIIRNVIGHCFSSRGGGYLFFGDDI 240

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YD +R+VWTPM     KHYSPG GEL+FNGRSTGLRNLF VFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDSHRVVWTPMSRDYPKHYSPGLGELIFNGRSTGLRNLFAVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKP KSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P+EGY+I+SSMGNVCLGILNGT+VGL+NSNIIGDISM DK+V+YNNEKQAIGWATANCDR
Sbjct: 361 PMEGYLILSSMGNVCLGILNGTEVGLQNSNIIGDISMHDKIVIYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSS 424
           VPKS+ ++
Sbjct: 421 VPKSRAAA 428

BLAST of IVF0004190 vs. ExPASy TrEMBL
Match: A0A6J1I590 (aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.9e-227
Identity = 379/430 (88.14%), Postives = 401/430 (93.26%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVP--TASSSFASSSIVLPL 60
           MGK VL++L LMV+S+SCLAPCSASSFFKDK WER+RP LSVP  +ASSS AS SIVLPL
Sbjct: 1   MGKGVLMILVLMVSSISCLAPCSASSFFKDKLWERRRPTLSVPIASASSSIASPSIVLPL 60

Query: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120
           QGNV+PNGFYNVTL++GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVFPNGFYNVTLFIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 240
           LGCGYDQ+PGSSSYHPMDG+LGLG+GAVSIVSQLHNQGIVRNVVGHCF+SKGGGYLFFGD
Sbjct: 181 LGCGYDQNPGSSSYHPMDGVLGLGKGAVSIVSQLHNQGIVRNVVGHCFSSKGGGYLFFGD 240

Query: 241 GIYDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYDPYRL WTPM     KHYSPGFG+L FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQ+
Sbjct: 241 DIYDPYRLAWTPMSRDYPKHYSPGFGDLFFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQI 300

Query: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 360
           +TSLLNREL GKPLREA DDDTLPLCWR R P KSLRDVRKYFKPLALSFSSG RSKAVF
Sbjct: 301 ITSLLNRELTGKPLREAKDDDTLPLCWRGRNPFKSLRDVRKYFKPLALSFSSGRRSKAVF 360

Query: 361 EIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           E+P E Y+IISS GNVCLGILNG++VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EMPTESYLIISSKGNVCLGILNGSEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSQVSS 424
           DRVPKS V S
Sbjct: 421 DRVPKSSVGS 430

BLAST of IVF0004190 vs. NCBI nr
Match: XP_008460823.1 (PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo])

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 424/429 (98.83%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 424
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of IVF0004190 vs. NCBI nr
Match: TYK02025.1 (aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 424/429 (98.83%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 42  MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 101

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 102 NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 161

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 162 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 221

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 222 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 281

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 282 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 341

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 342 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 401

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 402 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 461

Query: 421 VPKSQVSSW 424
           VPKSQVSSW
Sbjct: 462 VPKSQVSSW 470

BLAST of IVF0004190 vs. NCBI nr
Match: XP_008460822.1 (PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 424/433 (97.92%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 C----GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240
           C    GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF
Sbjct: 181 CQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240

Query: 241 GDGIYDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDGIYDPYRLVWTPM     KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300

Query: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360
           QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA
Sbjct: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360

Query: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420

Query: 421 NCDRVPKSQVSSW 424
           NCDRVPKSQVSSW
Sbjct: 421 NCDRVPKSQVSSW 433

BLAST of IVF0004190 vs. NCBI nr
Match: XP_004147327.2 (aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical protein Csa_016941 [Cucumis sativus])

HSP 1 Score: 864 bits (2232), Expect = 0.0
Identity = 419/429 (97.67%), Postives = 420/429 (97.90%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPM     KHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 424
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of IVF0004190 vs. NCBI nr
Match: XP_038900559.1 (aspartic proteinase Asp1 isoform X2 [Benincasa hispida])

HSP 1 Score: 840 bits (2170), Expect = 4.52e-307
Identity = 403/428 (94.16%), Postives = 415/428 (96.96%), Query Frame = 0

Query: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           M K VL++L LMVASMSCLAPCSASSFFKDKPWER+RPILSVP ASSSFASSSIV+PLQG
Sbjct: 1   MEKRVLMILVLMVASMSCLAPCSASSFFKDKPWERRRPILSVPIASSSFASSSIVMPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDG+LGLGRGAVS+VSQLHNQGIVRNVVGHCF+SKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGVLGLGRGAVSMVSQLHNQGIVRNVVGHCFSSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPM-----KHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYR+VWTPM     KHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRIVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKP KSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P+EGY+IISSMGN CLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PMEGYLIISSMGNACLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSS 423
           VPKS+V S
Sbjct: 421 VPKSRVGS 428

BLAST of IVF0004190 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 562.4 bits (1448), Expect = 3.1e-160
Identity = 255/373 (68.36%), Postives = 309/373 (82.84%), Query Frame = 0

Query: 50  ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP 109
           A SS+V P+ GNVYP G+YNVT+ +GQPP+PY+LD DTGSDLTWLQCDAPC +C E  HP
Sbjct: 42  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 101

Query: 110 LYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTN 169
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRDVF +N T 
Sbjct: 102 LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 161

Query: 170 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 229
           G  + PRLALGCGYDQ PG+SS+HP+DG+LGLGRG VSI+SQLH+QG V+NV+GHC +S 
Sbjct: 162 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 221

Query: 230 GGGYLFFGDGIYDPYRLVWTPM-----KHYSPGF-GELMFNGRSTGLRNLFVVFDSGSSY 289
           GGG LFFGD +YD  R+ WTPM     KHYSP   GEL+F GR+TGL+NL  VFDSGSSY
Sbjct: 222 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 281

Query: 290 TYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSF 349
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+ R+P  S+ +V+KYFKPLALSF
Sbjct: 282 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 341

Query: 350 SSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEK 409
            +G RSK +FEIP E Y+IIS  GNVCLGILNGT++GL+N N+IGDISMQD+M++Y+NEK
Sbjct: 342 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEK 401

Query: 410 QAIGWATANCDRV 417
           Q+IGW   +CD +
Sbjct: 402 QSIGWMPVDCDEL 414

BLAST of IVF0004190 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.4e-147
Identity = 238/345 (68.99%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 50  ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP 109
           A SS+V P+ GNVYP G+YNVT+ +GQPP+PY+LD DTGSDLTWLQCDAPC +C E  HP
Sbjct: 39  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 98

Query: 110 LYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTN 169
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRDVF +N T 
Sbjct: 99  LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 158

Query: 170 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 229
           G  + PRLALGCGYDQ PG+SS+HP+DG+LGLGRG VSI+SQLH+QG V+NV+GHC +S 
Sbjct: 159 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 218

Query: 230 GGGYLFFGDGIYDPYRLVWTPM-----KHYSPGF-GELMFNGRSTGLRNLFVVFDSGSSY 289
           GGG LFFGD +YD  R+ WTPM     KHYSP   GEL+F GR+TGL+NL  VFDSGSSY
Sbjct: 219 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 278

Query: 290 TYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSF 349
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+ R+P  S+ +V+KYFKPLALSF
Sbjct: 279 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 338

Query: 350 SSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIG 389
            +G RSK +FEIP E Y+IIS  GNVCLGILNGT++GL+N N+IG
Sbjct: 339 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIG 383

BLAST of IVF0004190 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.0e-118
Identity = 193/394 (48.98%), Postives = 280/394 (71.07%), Query Frame = 0

Query: 39  ILSVPTASSSF-------ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDL 98
           ++ VP + SS        + SS+V PL GNV+P G+Y+V + +G PPK +  D DTGSDL
Sbjct: 13  LVIVPLSKSSIFKTFIKSSPSSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDL 72

Query: 99  TWLQCDAPCQQCTETLHPLYQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYAD 158
           TW+QCDAPC  CT   +  Y+P  +++PC +P+C +LH      C NP +QCDYEV+YAD
Sbjct: 73  TWVQCDAPCSGCTLPPNLQYKPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYAD 132

Query: 159 GGSSLGVLVRDVFPLNLTNGDPIRPRLALGCGYDQDPGSSSYHPMD-GILGLGRGAVSIV 218
            GSS+G LV D FPL L NG  ++P +A GCGYDQ   S+   P   G+LGLGRG + ++
Sbjct: 133 QGSSMGALVTDQFPLKLVNGSFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLL 192

Query: 219 SQLHNQGIVRNVVGHCFNSKGGGYLFFGDGIYDPYRLVWTPM----KHYSPGFGELMFNG 278
           +QL + G+ RNVVGHC +SKGGG+LFFGD +     + WTP+     HY+ G  +L+FNG
Sbjct: 193 TQLVSAGLTRNVVGHCLSSKGGGFLFFGDNLVPSIGVAWTPLLSQDNHYTTGPADLLFNG 252

Query: 279 RSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKP 338
           + TGL+ L ++FD+GSSYTYFN++AYQ + +L+  +L   PL+ A +D TLP+CW+  KP
Sbjct: 253 KPTGLKGLKLIFDTGSSYTYFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKP 312

Query: 339 IKSLRDVRKYFKPLALSFSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSN 398
            KS+ +V+ +FK + ++F++G R+  ++  P E Y+I+S  GNVCLG+LNG++VGL+NSN
Sbjct: 313 FKSVLEVKNFFKTITINFTNGRRNTQLYLAP-ELYLIVSKTGNVCLGLLNGSEVGLQNSN 372

Query: 399 IIGDISMQDKMVVYNNEKQAIGWATANCDRVPKS 420
           +IGDISMQ  M++Y+NEKQ +GW +++C+++PK+
Sbjct: 373 VIGDISMQGLMMIYDNEKQQLGWVSSDCNKLPKT 405

BLAST of IVF0004190 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.4e-112
Identity = 192/378 (50.79%), Postives = 259/378 (68.52%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++V P+ GNVYP G+Y V L +G PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 111 YQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYADGGSSLGVLVRDVFPLNLTN 170
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 171 GDPIRPRLALGCGYD-QDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNS 230
           G  +  RL  GCGYD Q+PG     P  GILGLGRG V + +QL + GI +NV+ HC + 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 231 KGGGYLFFGDGIYDPYRLVWT------PMKHYSPGFGELMFNGRSTGLRNLFVVFDSGSS 290
            G G+L  GD +     + WT      P K+Y  G  EL+FN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 291 YTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALS 350
           YTYFNA+AYQ +  L+ ++L GKPL +  DD +LP+CW+ +KP+KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 351 FSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNE 410
           F +  ++  +F++P E Y+II+  G VCLGILNGT++GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 FGN-QKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 411 KQAIGWATANCDRVPKSQ 421
           KQ IGW +++CD++PKS+
Sbjct: 410 KQRIGWISSDCDKLPKSE 426

BLAST of IVF0004190 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 399.8 bits (1026), Expect = 2.7e-111
Identity = 190/375 (50.67%), Postives = 256/375 (68.27%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++V P+ GNVYP G+Y V L +G PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 111 YQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYADGGSSLGVLVRDVFPLNLTN 170
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 171 GDPIRPRLALGCGYD-QDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNS 230
           G  +  RL  GCGYD Q+PG     P  GILGLGRG V + +QL + GI +NV+ HC + 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 231 KGGGYLFFGDGIYDPYRLVWT------PMKHYSPGFGELMFNGRSTGLRNLFVVFDSGSS 290
            G G+L  GD +     + WT      P K+Y  G  EL+FN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 291 YTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALS 350
           YTYFNA+AYQ +  L+ ++L GKPL +  DD +LP+CW+ +KP+KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 351 FSSGGRSKAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNE 410
           F +  ++  +F++P E Y+II+  G VCLGILNGT++GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 FGN-QKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 411 KQAIGWATANCDRVP 418
           KQ IGW +++CD++P
Sbjct: 410 KQRIGWISSDCDKLP 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0IU524.3e-9046.65Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC672.5e-8544.42Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9M9A87.0e-8042.82Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q9S9K42.2e-3329.23Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q8VYV91.1e-2730.03Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BS691.2e-25298.83Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3CDB21.2e-25298.83aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A1S3CDB46.7e-25197.92aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A6J1DZ151.3e-22788.08aspartic proteinase Asp1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110243... [more]
A0A6J1I5903.9e-22788.14aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_008460823.10.098.83PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo][more]
TYK02025.10.098.83aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa][more]
XP_008460822.10.097.92PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo][more]
XP_004147327.20.097.67aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical... [more]
XP_038900559.14.52e-30794.16aspartic proteinase Asp1 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G33490.23.1e-16068.36Eukaryotic aspartyl protease family protein [more]
AT4G33490.11.4e-14768.99Eukaryotic aspartyl protease family protein [more]
AT1G44130.13.0e-11848.98Eukaryotic aspartyl protease family protein [more]
AT1G77480.21.4e-11250.79Eukaryotic aspartyl protease family protein [more]
AT1G77480.12.7e-11150.67Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 47..237
e-value: 2.5E-45
score: 156.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 245..417
e-value: 2.2E-26
score: 94.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 60..416
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 274..409
e-value: 5.5E-15
score: 55.5
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 68..238
e-value: 3.6E-48
score: 164.1
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 8..418
NoneNo IPR availablePANTHERPTHR13683:SF800EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 8..418
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..18
score: 5.0
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 68..409
score: 33.204533

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0004190.2IVF0004190.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity