IVF0003750 (gene) Melon (IVF77) v1

Overview
NameIVF0003750
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionmediator of RNA polymerase II transcription subunit 32
Locationchr11: 4170536 .. 4170974 (+)
RNA-Seq ExpressionIVF0003750
SyntenyIVF0003750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTTCTTGAAGCAAATGAAGCCTGGGGCTCAGAAGACGGCAGCAACAGATGCCGCTCTTGAGAATTTTAAACAAAAATGGGAGCTGTTCAGGGTTGCTTGTGATCAAGCAGAGGAGTTTGTAGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCCTTGTTGATGAGGCCACTGGTTCACTGGCTGGCAAGTCTGGGCAGTCTGGTACCACTGGCCTTCCTCCGATCAGTGCTGTTCGATTGGAACAGATGAGCAAAGCTGTAAGATGGCTCGTGATTGAGTTGCAGCACGGCTCAGGAGCTGCTGCTTCTGCTTCAGCACACTCTCATCAGTCTGCTCCTTTTGATGCTAGATTTACTGAGGATACAACCCAATAG

mRNA sequence

ATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTTCTTGAAGCAAATGAAAATTTTAAACAAAAATGGGAGCTGTTCAGGGTTGCTTGTGATCAAGCAGAGGAGTTTGTAGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCCTTGTTGATGAGGCCACTGGTTCACTGGCTGGCAAGTCTGGGCAGTCTGGTACCACTGGCCTTCCTCCGATCAGTGCTGTTCGATTGGAACAGATGAGCAAAGCTGTAAGATGGCTCGTGATTGAGTTGCAGCACGGCTCAGGAGCTGCTGCTTCTGCTTCAGCACACTCTCATCAGTCTGCTCCTTTTGATGCTAGATTTACTGAGGATACAACCCAATAG

Coding sequence (CDS)

ATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTTCTTGAAGCAAATGAAAATTTTAAACAAAAATGGGAGCTGTTCAGGGTTGCTTGTGATCAAGCAGAGGAGTTTGTAGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCCTTGTTGATGAGGCCACTGGTTCACTGGCTGGCAAGTCTGGGCAGTCTGGTACCACTGGCCTTCCTCCGATCAGTGCTGTTCGATTGGAACAGATGAGCAAAGCTGTAAGATGGCTCGTGATTGAGTTGCAGCACGGCTCAGGAGCTGCTGCTTCTGCTTCAGCACACTCTCATCAGTCTGCTCCTTTTGATGCTAGATTTACTGAGGATACAACCCAATAG

Protein sequence

MDSLVDSLNNAYQEFVAGAANVLEANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
Homology
BLAST of IVF0003750 vs. ExPASy Swiss-Prot
Match: Q84VW5 (Mediator of RNA polymerase II transcription subunit 32 OS=Arabidopsis thaliana OX=3702 GN=MED32 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-38
Identity = 96/153 (62.75%), Postives = 109/153 (71.24%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEE 60
           MD++VDSLN AY++FV  +A VLE               A ENFK+KWELFRVACDQAEE
Sbjct: 1   MDNIVDSLNKAYEKFVLASAGVLESKESAGGQKALLTDTALENFKEKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----LPPISAVRLEQMSKAVRWLV 120
           FVESVKQRIGSECLVDEATG   + AG    +  TG    LPPISAVRLEQMS+AVRWLV
Sbjct: 61  FVESVKQRIGSECLVDEATGLTTTAAGGQAPAAVTGAATSLPPISAVRLEQMSRAVRWLV 120

Query: 121 IELQHGSGAAASASAHSHQSAPFDARFTEDTTQ 132
           +ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Sbjct: 121 LELQRGSG-VAPGSVHS-SSTGFDSRFSEDSTQ 151

BLAST of IVF0003750 vs. ExPASy TrEMBL
Match: A0A5D3CQ46 (Mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001830 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.0e-61
Identity = 131/146 (89.73%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEE
Sbjct: 5   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 64

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 65  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 124

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 132
           GAAASASAHSHQSAPFDARFTEDTTQ
Sbjct: 125 GAAASASAHSHQSAPFDARFTEDTTQ 150

BLAST of IVF0003750 vs. ExPASy TrEMBL
Match: A0A1S3C6T6 (mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo OX=3656 GN=LOC103497499 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.0e-61
Identity = 131/146 (89.73%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 132
           GAAASASAHSHQSAPFDARFTEDTTQ
Sbjct: 121 GAAASASAHSHQSAPFDARFTEDTTQ 146

BLAST of IVF0003750 vs. ExPASy TrEMBL
Match: A0A5A7TWZ7 (Mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G004640 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.2e-60
Identity = 130/146 (89.04%), Postives = 130/146 (89.04%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEE
Sbjct: 5   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 64

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 65  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 124

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 132
           GAAASASAHSHQSAPFDARFTED TQ
Sbjct: 125 GAAASASAHSHQSAPFDARFTEDATQ 150

BLAST of IVF0003750 vs. ExPASy TrEMBL
Match: A0A0A0LIN7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G245440 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.8e-59
Identity = 128/146 (87.67%), Postives = 128/146 (87.67%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAVRLEQMSKAVRWLVIELQHGS 120

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 132
           GAAASASAHSHQSAPFDARFTED TQ
Sbjct: 121 GAAASASAHSHQSAPFDARFTEDATQ 146

BLAST of IVF0003750 vs. ExPASy TrEMBL
Match: A0A6J1JH28 (mediator of RNA polymerase II transcription subunit 32-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484308 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.5e-55
Identity = 125/149 (83.89%), Postives = 128/149 (85.91%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFV+GAANVLEAN               ENFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVSGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSG--TTGLPPISAVRLEQMSKAVRWLVIELQH 120
           FVESVKQRIGSECLVDEATGSLAGKSGQ+   TTGLPPISA+RLEQMSKAVRWLVIELQH
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQAAATTTGLPPISAIRLEQMSKAVRWLVIELQH 120

Query: 121 GSG-AAASASAHSHQSAPFDARFTEDTTQ 132
           GSG AAASASAHSH SAPFDARFTED TQ
Sbjct: 121 GSGAAAASASAHSHPSAPFDARFTEDATQ 149

BLAST of IVF0003750 vs. NCBI nr
Match: XP_008457936.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 32 [Cucumis melo])

HSP 1 Score: 244 bits (622), Expect = 9.52e-81
Identity = 131/146 (89.73%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 131
           GAAASASAHSHQSAPFDARFTEDTTQ
Sbjct: 121 GAAASASAHSHQSAPFDARFTEDTTQ 146

BLAST of IVF0003750 vs. NCBI nr
Match: TYK13665.1 (mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa])

HSP 1 Score: 244 bits (622), Expect = 1.09e-80
Identity = 131/146 (89.73%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEE
Sbjct: 5   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 64

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 65  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 124

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 131
           GAAASASAHSHQSAPFDARFTEDTTQ
Sbjct: 125 GAAASASAHSHQSAPFDARFTEDTTQ 150

BLAST of IVF0003750 vs. NCBI nr
Match: KAA0045925.1 (mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa])

HSP 1 Score: 242 bits (617), Expect = 6.30e-80
Identity = 130/146 (89.04%), Postives = 130/146 (89.04%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEE
Sbjct: 5   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 64

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 65  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 124

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 131
           GAAASASAHSHQSAPFDARFTED TQ
Sbjct: 125 GAAASASAHSHQSAPFDARFTEDATQ 150

BLAST of IVF0003750 vs. NCBI nr
Match: XP_038902813.1 (mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida] >XP_038902814.1 mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida])

HSP 1 Score: 238 bits (606), Expect = 2.62e-78
Identity = 127/146 (86.99%), Postives = 129/146 (88.36%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQ+ TTG+PPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQAATTGIPPISAVRLEQMSKAVRWLVIELQHGS 120

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 131
           GAAASASAHSHQSAPFDARFTED TQ
Sbjct: 121 GAAASASAHSHQSAPFDARFTEDATQ 146

BLAST of IVF0003750 vs. NCBI nr
Match: XP_004148855.1 (mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus] >XP_031736355.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus] >KGN61780.1 hypothetical protein Csa_006151 [Cucumis sativus])

HSP 1 Score: 237 bits (605), Expect = 3.72e-78
Identity = 128/146 (87.67%), Postives = 128/146 (87.67%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEE 60
           MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEE
Sbjct: 1   MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGS 120
           FVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAVRLEQMSKAVRWLVIELQHGS
Sbjct: 61  FVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAVRLEQMSKAVRWLVIELQHGS 120

Query: 121 GAAASASAHSHQSAPFDARFTEDTTQ 131
           GAAASASAHSHQSAPFDARFTED TQ
Sbjct: 121 GAAASASAHSHQSAPFDARFTEDATQ 146

BLAST of IVF0003750 vs. TAIR 10
Match: AT1G11760.1 (MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 160.2 bits (404), Expect = 1.1e-39
Identity = 96/153 (62.75%), Postives = 109/153 (71.24%), Query Frame = 0

Query: 1   MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEE 60
           MD++VDSLN AY++FV  +A VLE               A ENFK+KWELFRVACDQAEE
Sbjct: 1   MDNIVDSLNKAYEKFVLASAGVLESKESAGGQKALLTDTALENFKEKWELFRVACDQAEE 60

Query: 61  FVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----LPPISAVRLEQMSKAVRWLV 120
           FVESVKQRIGSECLVDEATG   + AG    +  TG    LPPISAVRLEQMS+AVRWLV
Sbjct: 61  FVESVKQRIGSECLVDEATGLTTTAAGGQAPAAVTGAATSLPPISAVRLEQMSRAVRWLV 120

Query: 121 IELQHGSGAAASASAHSHQSAPFDARFTEDTTQ 132
           +ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Sbjct: 121 LELQRGSG-VAPGSVHS-SSTGFDSRFSEDSTQ 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84VW51.6e-3862.75Mediator of RNA polymerase II transcription subunit 32 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A5D3CQ463.0e-6189.73Mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo var. maku... [more]
A0A1S3C6T63.0e-6189.73mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo OX=3656 G... [more]
A0A5A7TWZ71.2e-6089.04Mediator of RNA polymerase II transcription subunit 32 OS=Cucumis melo var. maku... [more]
A0A0A0LIN72.8e-5987.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G245440 PE=4 SV=1[more]
A0A6J1JH281.5e-5583.89mediator of RNA polymerase II transcription subunit 32-like isoform X2 OS=Cucurb... [more]
Match NameE-valueIdentityDescription
XP_008457936.19.52e-8189.73PREDICTED: mediator of RNA polymerase II transcription subunit 32 [Cucumis melo][more]
TYK13665.11.09e-8089.73mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa... [more]
KAA0045925.16.30e-8089.04mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa... [more]
XP_038902813.12.62e-7886.99mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida] >XP_0... [more]
XP_004148855.13.72e-7887.67mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus] >XP_031... [more]
Match NameE-valueIdentityDescription
AT1G11760.11.1e-3962.75MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0;... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..131
NoneNo IPR availablePANTHERPTHR35989:SF1MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 32coord: 1..131
IPR033244Mediator of RNA polymerase II transcription subunit 32PANTHERPTHR35989MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 32coord: 1..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0003750.1IVF0003750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009631 cold acclimation
biological_process GO:0010150 leaf senescence
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0048364 root development
cellular_component GO:0016592 mediator complex