IVF0002971 (gene) Melon (IVF77) v1

BLAST of IVF0002971 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.1e-183
Identity = 336/529 (63.52%), Postives = 404/529 (76.37%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSE 133
           TCTFTESI+RN GFRTGLFTSPHLID+RERFRLDG+DISEEKFL YFW CYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHG 253
           +LG+TLG+IA EKAGIFK   PAFTV QPDEAMRVL++KAS+ +V L VV  L  +LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NAGLAV+L+S WL++ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSS 373
           +VPD+YT+  +S DLVFYLDGAHSPESMEAC +WFS AV        K  ++   SG   
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAV--------KGDNQSGSSGHLV 425

Query: 374 NKLQGYDNDE-ARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVF 433
           N   G  +D+ + +   Q+LLFNCM+VRDP LLLP L   CA  GVNFKKALFVPN SV+
Sbjct: 426 NGSAGSSHDKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVY 485

Query: 434 NKVGTHG-LRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKN 493
           +KVGT   L E D QVDLSWQF ++++WE+                L +     E  G++
Sbjct: 486 HKVGTAADLPENDPQVDLSWQFTLQKVWES----------------LVQSERDGEKDGES 545

Query: 494 CENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
             NS VF+SLP+AIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 DGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of IVF0002971 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 2.4e-162
Identity = 305/526 (57.98%), Postives = 377/526 (71.67%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSED 134
           CTF ESILR  G RTGLFTSPHLID+RERFRL+GI+IS+EKF+ YFWCC+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHGR 254
           LG TL EIA+EKAGIFK   PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLAVAL ST+L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSSN 374
           VPD     E  +++V+YLDGAHSPESMEAC  WFS  ++ NQ  N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 KLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVFNK 434
                     +K+S Q+LLFNCM+VRDP LLLP L   C + GV+FK+A+FVPN SV+N+
Sbjct: 373 ----------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQ 432

Query: 435 VGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKNCEN 494
           VG+     T  +  +SWQF ++RIWE+    +  S+SK  S             GK  E 
Sbjct: 433 VGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 491

Query: 495 STVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           S VFSSLP+A+ WLRD+  +Q++ VR QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of IVF0002971 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 294/555 (52.97%), Postives = 385/555 (69.37%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKG 73
           +Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTKG
Sbjct: 82  SYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTKG 141

Query: 74  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 133
           KGSTC F+E+ILRNCGFRTG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKEK
Sbjct: 142 KGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEK 201

Query: 134 TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 193
             + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P+VCGIASLG D
Sbjct: 202 AVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGMD 261

Query: 194 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 253
           HM++LGNTL +IA  KAGIFK + PAFTV Q  EAM VL+  A+ L+V L VV  L+PK 
Sbjct: 262 HMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKK 321

Query: 254 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSR--CLPEQFIEGLTTASF 313
           L G  LGL G+HQ +NAGLAV+LS  WL+RTG+ +  +  +S+   +P  F  GL TA  
Sbjct: 322 LDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARL 381

Query: 314 EGRAQIVPDKYTDVESSD--------DLVFYLDGAHSPESMEACGRWFSAAVRHNQ-LGN 373
            GRAQ+V D  +D + S         DL+FYLDGAHSPESMEACGRWFS+AVR ++ L  
Sbjct: 382 HGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDKSLST 441

Query: 374 FKNQSEDNGS-GTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGV 433
             N    +G  GT  N++           S Q+LLFNCM VRDPQ+LLP L+ TCA++G 
Sbjct: 442 AVNGYMRHGEYGTDLNRV-----------SKQILLFNCMEVRDPQVLLPKLVTTCASSGT 501

Query: 434 NFKKALFVPNTSVFNKV--GTHGLRETDGQVDLSWQFHVRRIWENT-KPCDKGSD----S 493
           +F +ALFVP+ S +NKV  G   +     + DL+WQF ++R+WE + +  D G D     
Sbjct: 502 HFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKP 561

Query: 494 KGTSAVLEEE---SEKAEVG---GKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLV 539
            G +A+   +    +  + G   G    +S V  SLPL I WLRD V ++N S++L+VLV
Sbjct: 562 DGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLV 621

BLAST of IVF0002971 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 8.5e-83
Identity = 212/589 (35.99%), Postives = 302/589 (51.27%), Query Frame = 0

Query: 8   LKDSQMAYEEALDALSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-ESMSQMK 67
           L++  M Y++A+  L++L  N    +    K GD   + E M  Y+    L+ E + Q+ 
Sbjct: 35  LQEPDMEYQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLN 94

Query: 68  VIHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 127
           +IH+ GTKGKGSTC FTE ILR+ G +TG F+SPHL+ +RER R++G  IS E F  +FW
Sbjct: 95  IIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFW 154

Query: 128 CCYNRLKEKTSED--IPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAP 187
             Y+RL+E   +   + MP  FRFL L+AF +F  E+VD+A+LEVG+GG +D TN+++ P
Sbjct: 155 RLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKP 214

Query: 188 IVCGIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQ 247
           +VCGI SLG DH  LLG+T+ +IA +K GIFK   PAFTV QPDE + VL+D+A Q+   
Sbjct: 215 VVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCP 274

Query: 248 LTVVPSLDPKLLHG--RKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSR-CLP 307
           L + P L      G    LGLEGEHQ  NA LA+ ++  WL++ G+     ++ SR  LP
Sbjct: 275 LYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLP 334

Query: 308 EQ------------FIEGLTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEAC 367
            Q              +GL    + GR Q++            L +YLDGAH+  SM+AC
Sbjct: 335 GQLPLAPVFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQAC 394

Query: 368 GRWFSAAVRHNQLGNFKNQSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQL 427
            RWF  A+        + +  D+GS                   V+VLLFN    RD   
Sbjct: 395 VRWFRQALH-------RCERPDSGS------------------EVRVLLFNSTGDRDSAA 454

Query: 428 LLPPLIKTCANNGVNFKKALFVPNTSVFNKVGTHGLRETDGQVD------LSWQFHVRRI 487
           LL  L++ C      F  A+F PN +          +     +D      L+ Q H  R+
Sbjct: 455 LL-KLLQPC-----QFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRL 514

Query: 488 WENTKPCD----KGSDSKGTSAVLEEESEKAEVGGKNCENSTVFSSLPLAIKWL---RDS 539
            E     D     G +  G +++L            +  +S VFS +  A++W+   RD 
Sbjct: 515 HEEQVSPDPWSTPGQEQDGPASLLLAPHPP----HTHSASSLVFSCISHALQWITQGRDP 574

BLAST of IVF0002971 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 302.4 bits (773), Expect = 1.0e-80
Identity = 204/575 (35.48%), Postives = 291/575 (50.61%), Query Frame = 0

Query: 13  MAYEEALDALSSLIVN--------RRRCDKSGDRFELMFEYVKMLDLE-ESMSQMKVIHV 72
           M Y++A+  L++L  N        R+R D    + E M  Y+    L+ E ++++ +IHV
Sbjct: 43  MEYQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHV 102

Query: 73  AGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYN 132
            GTKGKGSTC FTE ILRN G +TG F+SPH++ +RER R++G  IS E F  +FWC YN
Sbjct: 103 TGTKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYN 162

Query: 133 RLKE-KTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 192
           +L+E K    + MP  FRFL L+AF +F  E+VD+A++EVG+GG FD TN+++ P+VCG+
Sbjct: 163 QLEEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGV 222

Query: 193 ASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVP 252
           +SLG DH  LLG+T+ +IA +K GIFK   PAFTV QP+  + VL+D+A Q+   L + P
Sbjct: 223 SSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCP 282

Query: 253 SLDPKLLHG--RKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSR-----CLPE 312
            L+     G    LGLEG HQ  NA LA+ L+  WL R  H  +  ++ SR      LP 
Sbjct: 283 PLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPL 342

Query: 313 QFIEGLTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQL 372
             +   T     G    V    T +     L +YLDGAH+  S++AC  W+  ++     
Sbjct: 343 APVFRPTPHMRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWYRQSLE---- 402

Query: 373 GNFKNQSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNG 432
              +++  D GS                   V +LLFN    RD   LL  L++ C    
Sbjct: 403 ---RSKRTDGGS------------------EVHILLFNSTGDRDSAALL-KLLQPC---- 462

Query: 433 VNFKKALFVPNTSVFNKVGTHGLRETDGQVD------LSWQFHVRRIWENTKPCDK---- 492
             F  A+F PN +  + +G    +     +D      L  Q H   + E     +     
Sbjct: 463 -QFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSNLWSSC 522

Query: 493 GSDSKGTSAVLEEESEKAEVGGKNCENSTVFSSLPLAIKWL---RDSVLQQ--------- 538
           G D  G  ++L               +S VFS +  A+ W+   RD + Q          
Sbjct: 523 GPDPAGPGSLLLAPHPPQPTR----TSSLVFSCISHALLWISQGRDPIFQPQSLPRNLLN 581

BLAST of IVF0002971 vs. ExPASy TrEMBL
Match: A0A1S3C839 (Folylpolyglutamate synthase OS=Cucumis melo OX=3656 GN=LOC103498061 PE=3 SV=1)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES
Sbjct: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538

BLAST of IVF0002971 vs. ExPASy TrEMBL
Match: A0A5A7T3F3 (Folylpolyglutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00840 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.7e-302
Identity = 520/522 (99.62%), Postives = 521/522 (99.81%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES
Sbjct: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVL 523
           EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQ +
Sbjct: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQTI 522

BLAST of IVF0002971 vs. ExPASy TrEMBL
Match: A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.1e-298
Identity = 516/538 (95.91%), Postives = 526/538 (97.77%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEESMSQMKV
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKE+TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKR APAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDP+LLHGRKLGLEGEHQYLNAGLAVALSSTWL R+GHS+LSYVEQSR LPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPD+YTDVE  D+LVFYLDGAHSPESMEACGRWFSAAVRHNQLGN  +
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 376 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 435

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGL ETD QVDLSWQFHVRRIWE+TK CDKGSDSKGT AVLEEES
Sbjct: 436 ALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEES 495

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           EK+EVGGKNC NSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 EKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of IVF0002971 vs. ExPASy TrEMBL
Match: A0A1S4E2X5 (folylpolyglutamate synthase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498061 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 3.0e-272
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 69  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 128
           KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK
Sbjct: 17  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 76

Query: 129 TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 188
           TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD
Sbjct: 77  TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 136

Query: 189 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 248
           HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL
Sbjct: 137 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 196

Query: 249 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG 308
           LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG
Sbjct: 197 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG 256

Query: 309 RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG 368
           RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG
Sbjct: 257 RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG 316

Query: 369 TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS 428
           TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS
Sbjct: 317 TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS 376

Query: 429 VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK 488
           VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK
Sbjct: 377 VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK 436

Query: 489 NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 437 NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 486

BLAST of IVF0002971 vs. ExPASy TrEMBL
Match: A0A1S3C8P3 (Folylpolyglutamate synthase OS=Cucumis melo OX=3656 GN=LOC103498061 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDK 466
           ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDK
Sbjct: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDK 465

BLAST of IVF0002971 vs. NCBI nr
Match: XP_008458746.1 (PREDICTED: folylpolyglutamate synthase isoform X1 [Cucumis melo])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES
Sbjct: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538
           EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538

BLAST of IVF0002971 vs. NCBI nr
Match: KAA0037503.1 (folylpolyglutamate synthase isoform X1 [Cucumis melo var. makuwa] >TYK01978.1 folylpolyglutamate synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES
Sbjct: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQ 520
           EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQ
Sbjct: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQ 520

BLAST of IVF0002971 vs. NCBI nr
Match: XP_004142354.1 (folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein Csa_008061 [Cucumis sativus])

HSP 1 Score: 1031 bits (2667), Expect = 0.0
Identity = 516/538 (95.91%), Postives = 526/538 (97.77%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEESMSQMKV
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKE+TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFKR APAFTVFQPDEAMRVLKDKASQLDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           VPSLDP+LLHGRKLGLEGEHQYLNAGLAVALSSTWL R+GHS+LSYVEQSR LPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPD+YTDVE  D+LVFYLDGAHSPESMEACGRWFSAAVRHNQLGN  +
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK
Sbjct: 376 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 435

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEES 480
           ALFVPNTSVFNKVGTHGL ETD QVDLSWQFHVRRIWE+TK CDKGSDSKGT AVLEEES
Sbjct: 436 ALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEES 495

Query: 481 EKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538
           EK+EVGGKNC NSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 EKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of IVF0002971 vs. NCBI nr
Match: XP_038889970.1 (folylpolyglutamate synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 491/540 (90.93%), Postives = 509/540 (94.26%), Query Frame = 0

Query: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEGNETLKDS+ AYEEALDALS LIVNRRRCDKSGDRFELMFEYVKMLDLEESM QMKV
Sbjct: 1   MVEGNETLKDSRTAYEEALDALSLLIVNRRRCDKSGDRFELMFEYVKMLDLEESMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120
           IHVAGTKGKGSTCTFTESILRN GF TGLFTSPHLIDIRERFRLDG+DISEEKFLTYFW 
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNYGFHTGLFTSPHLIDIRERFRLDGVDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSED+PMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAP+VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPVVC 180

Query: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLGEIASEKAGIFK  APAFTVFQPDEAMRVL+DKASQLDVQLT 
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKHGAPAFTVFQPDEAMRVLRDKASQLDVQLTT 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300
           V  LDP+LLHGRKLGLEGEHQYLNAGLA+ALSSTWL R GHS+LSYVEQ+R LPEQFIEG
Sbjct: 241 VSPLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSELSYVEQNRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360
           LTTASFEGRAQIVPDKY DVES DDLVFYLDGAHSPESMEACGRWFSAA+RHNQ GN  N
Sbjct: 301 LTTASFEGRAQIVPDKYIDVESPDDLVFYLDGAHSPESMEACGRWFSAAIRHNQPGNL-N 360

Query: 361 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 420
           QSEDN   TSSNKLQGYDNDEARKKSVQVLLFNCM+VRDPQLLLPPLIK CANNGVNFKK
Sbjct: 361 QSEDNA--TSSNKLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLPPLIKMCANNGVNFKK 420

Query: 421 ALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWE--NTKPCDKGSDSKGTSAVLEE 480
           ALFVPNTSVFNKVGTHGL ET+GQVDLSWQFH+RR+WE  NT  C KG+DSKGT  VL+E
Sbjct: 421 ALFVPNTSVFNKVGTHGLPETNGQVDLSWQFHLRRVWEKENTMQCVKGNDSKGTGVVLQE 480

Query: 481 ESEKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538
           ESEK+EVGGKNCENSTVFSSLP+AIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 ESEKSEVGGKNCENSTVFSSLPMAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 537

BLAST of IVF0002971 vs. NCBI nr
Match: XP_016902330.1 (PREDICTED: folylpolyglutamate synthase isoform X2 [Cucumis melo])

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 69  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 128
           KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK
Sbjct: 17  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 76

Query: 129 TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 188
           TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD
Sbjct: 77  TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 136

Query: 189 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 248
           HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL
Sbjct: 137 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 196

Query: 249 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG 308
           LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG
Sbjct: 197 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEG 256

Query: 309 RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG 368
           RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG
Sbjct: 257 RAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSG 316

Query: 369 TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS 428
           TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS
Sbjct: 317 TSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTS 376

Query: 429 VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK 488
           VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK
Sbjct: 377 VFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGK 436

Query: 489 NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538
           NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK
Sbjct: 437 NCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 486

BLAST of IVF0002971 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 644.4 bits (1661), Expect = 8.0e-185
Identity = 336/529 (63.52%), Postives = 404/529 (76.37%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSE 133
           TCTFTESI+RN GFRTGLFTSPHLID+RERFRLDG+DISEEKFL YFW CYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHG 253
           +LG+TLG+IA EKAGIFK   PAFTV QPDEAMRVL++KAS+ +V L VV  L  +LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NAGLAV+L+S WL++ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSS 373
           +VPD+YT+  +S DLVFYLDGAHSPESMEAC +WFS AV        K  ++   SG   
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAV--------KGDNQSGSSGHLV 425

Query: 374 NKLQGYDNDE-ARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVF 433
           N   G  +D+ + +   Q+LLFNCM+VRDP LLLP L   CA  GVNFKKALFVPN SV+
Sbjct: 426 NGSAGSSHDKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVY 485

Query: 434 NKVGTHG-LRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKN 493
           +KVGT   L E D QVDLSWQF ++++WE+                L +     E  G++
Sbjct: 486 HKVGTAADLPENDPQVDLSWQFTLQKVWES----------------LVQSERDGEKDGES 545

Query: 494 CENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
             NS VF+SLP+AIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 DGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of IVF0002971 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 644.4 bits (1661), Expect = 8.0e-185
Identity = 336/529 (63.52%), Postives = 404/529 (76.37%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 74  TCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSE 133
           TCTFTESI+RN GFRTGLFTSPHLID+RERFRLDG+DISEEKFL YFW CYNRLKE+T+E
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 134 DIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDHME
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 194 LLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHG 253
           +LG+TLG+IA EKAGIFK   PAFTV QPDEAMRVL++KAS+ +V L VV  L  +LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 254 RKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NAGLAV+L+S WL++ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 314 IVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSS 373
           +VPD+YT+  +S DLVFYLDGAHSPESMEAC +WFS AV        K  ++   SG   
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAV--------KGDNQSGSSGHLV 367

Query: 374 NKLQGYDNDE-ARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVF 433
           N   G  +D+ + +   Q+LLFNCM+VRDP LLLP L   CA  GVNFKKALFVPN SV+
Sbjct: 368 NGSAGSSHDKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVY 427

Query: 434 NKVGTHG-LRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKN 493
           +KVGT   L E D QVDLSWQF ++++WE+                L +     E  G++
Sbjct: 428 HKVGTAADLPENDPQVDLSWQFTLQKVWES----------------LVQSERDGEKDGES 487

Query: 494 CENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
             NS VF+SLP+AIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 DGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

BLAST of IVF0002971 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-163
Identity = 305/526 (57.98%), Postives = 377/526 (71.67%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSED 134
           CTF ESILR  G RTGLFTSPHLID+RERFRL+GI+IS+EKF+ YFWCC+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHGR 254
           LG TL EIA+EKAGIFK   PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLAVAL ST+L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSSN 374
           VPD     E  +++V+YLDGAHSPESMEAC  WFS  ++ NQ  N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 KLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVFNK 434
                     +K+S Q+LLFNCM+VRDP LLLP L   C + GV+FK+A+FVPN SV+N+
Sbjct: 373 ----------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQ 432

Query: 435 VGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKNCEN 494
           VG+     T  +  +SWQF ++RIWE+    +  S+SK  S             GK  E 
Sbjct: 433 VGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 491

Query: 495 STVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           S VFSSLP+A+ WLRD+  +Q++ VR QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of IVF0002971 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 567.4 bits (1461), Expect = 1.2e-161
Identity = 304/526 (57.79%), Postives = 376/526 (71.48%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEKTSED 134
           CTF ESILR  G RTGLFTSPHLID+RERFRL+GI+IS+EKF+ YFWCC+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATN +Q P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKLLHGR 254
           LG TL EIA+EKAGIFK   PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLAVAL ST+L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKNQSEDNGSGTSSN 374
           VPD     E  +++V+YLDGAHSPESMEAC  WFS  ++ NQ  N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 KLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKKALFVPNTSVFNK 434
                     +K+S Q+LLFNCM+VRDP LLLP L   C + GV+FK+A+FVPN SV+N+
Sbjct: 373 ----------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQ 432

Query: 435 VGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEESEKAEVGGKNCEN 494
           VG+     T  +  +SWQF ++RIWE+    +  S+SK  S             GK  E 
Sbjct: 433 VGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 490

Query: 495 STVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 539
           S VFSSLP+A+ WLRD+  +Q++ VR QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 490

BLAST of IVF0002971 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 534.6 bits (1376), Expect = 8.9e-152
Identity = 294/555 (52.97%), Postives = 385/555 (69.37%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKG 73
           +Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTKG
Sbjct: 82  SYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTKG 141

Query: 74  KGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCYNRLKEK 133
           KGSTC F+E+ILRNCGFRTG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKEK
Sbjct: 142 KGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEK 201

Query: 134 TSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYD 193
             + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P+VCGIASLG D
Sbjct: 202 AVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGMD 261

Query: 194 HMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTVVPSLDPKL 253
           HM++LGNTL +IA  KAGIFK + PAFTV Q  EAM VL+  A+ L+V L VV  L+PK 
Sbjct: 262 HMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKK 321

Query: 254 LHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSR--CLPEQFIEGLTTASF 313
           L G  LGL G+HQ +NAGLAV+LS  WL+RTG+ +  +  +S+   +P  F  GL TA  
Sbjct: 322 LDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARL 381

Query: 314 EGRAQIVPDKYTDVESSD--------DLVFYLDGAHSPESMEACGRWFSAAVRHNQ-LGN 373
            GRAQ+V D  +D + S         DL+FYLDGAHSPESMEACGRWFS+AVR ++ L  
Sbjct: 382 HGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDKSLST 441

Query: 374 FKNQSEDNGS-GTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGV 433
             N    +G  GT  N++           S Q+LLFNCM VRDPQ+LLP L+ TCA++G 
Sbjct: 442 AVNGYMRHGEYGTDLNRV-----------SKQILLFNCMEVRDPQVLLPKLVTTCASSGT 501

Query: 434 NFKKALFVPNTSVFNKV--GTHGLRETDGQVDLSWQFHVRRIWENT-KPCDKGSD----S 493
           +F +ALFVP+ S +NKV  G   +     + DL+WQF ++R+WE + +  D G D     
Sbjct: 502 HFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKP 561

Query: 494 KGTSAVLEEE---SEKAEVG---GKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLV 539
            G +A+   +    +  + G   G    +S V  SLPL I WLRD V ++N S++L+VLV
Sbjct: 562 DGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLV 621