IVF0001661 (gene) Melon (IVF77) v1

BLAST of IVF0001661 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.4e-140
Identity = 253/513 (49.32%), Postives = 339/513 (66.08%), Query Frame = 0

Query: 33  KDFLRCLSIH-----STDIPIYTPINSSYTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHV 92
           ++FL+C S H     +    +YT  +  Y S L S  QN RF +   T KPL I+ PS+ 
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD-TTPKPLVIVTPSNN 91

Query: 93  SHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFIVVDLINLKSIEVDTTNNTVWVQ 152
           SHIQ+T++CSK  GLQIR RSGGHD EG+SYIS +PF+VVDL N+ SI++D  + T WV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 153 SGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLV 212
           +GAT+GE+YY I EK+  L+FP G CPTVGVGG FSGGGYG LMR YGLAADN+IDA+LV
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTK 272
           + +G V D ++MGE LFWAIRGGGG +FGI+  WK+KLV VP   T   + K+++     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271

Query: 273 KIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLIKIMNT 332
           K+  +WQ +A K +++L++  +    N T    +N T     F S++ G  D L+ +MN 
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331

Query: 333 TFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLTKPTLSNNISYKIKSDYVK 392
           +FP LG+ K +CKE SWI +T+  +G  N        EILL + +     ++ IK DYVK
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR-SAGKKTAFSIKLDYVK 391

Query: 393 QPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSKTPFSHRAEFLYKISYTVGW 452
           +PI E A   I ++L   +V      L+PYGG M  IS S  PF HRA  +Y++ YT  W
Sbjct: 392 KPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW 451

Query: 453 AEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLDIGTNNKCGKTSYEEASIWG 512
            +Q    NE+H+NW+R +Y+F TP+VS +PR AY+NYRDLD+G  N     +Y +A IWG
Sbjct: 452 EKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWG 511

Query: 513 MKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPL 533
            KYFG NF RLV+VKT VDP+NFFR++QSIPPL
Sbjct: 512 EKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of IVF0001661 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 256/520 (49.23%), Postives = 348/520 (66.92%), Query Frame = 0

Query: 26  TASSYKNKDFLRCLSIH----STDIP-IYTPINSSYTSTLRSYAQNPRFSTQYKTLKPLA 85
           T+ +   ++FL+C S +    +T++  +YT  N  Y S L S   N RF T   T KPL 
Sbjct: 25  TSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRF-TSDTTPKPLV 84

Query: 86  IIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFIVVDLINLKSIEVDTT 145
           I+ PSHVSHIQ T++CSK  GLQIR RSGGHD+EG+SYIS +PF++VDL N++SI++D  
Sbjct: 85  IVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVH 144

Query: 146 NNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSGGGYGWLMRKYGLAADN 205
           + T WV++GAT+GE+YY + EK+  L+  AG CPTV  GG F GGGYG LMR YGLAADN
Sbjct: 145 SQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADN 204

Query: 206 VIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKS 265
           +IDA+LV+ +G V D ++MGE LFWA+RGGG  SFGI+V WK++LV VP   T   + K 
Sbjct: 205 IIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK-STMFSVKKI 264

Query: 266 LD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTA---SFFSLYLGKTDK 325
           ++     K+V +WQ +A K +++LL+  +    N T    +N TA    F S++LG  D 
Sbjct: 265 MEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDS 324

Query: 326 LIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQP----LEILLTKPTLSNNISYK 385
           L+ +MN +FP LG+ K +C++ SWI + +  +G  N        EILL + +   N ++K
Sbjct: 325 LVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDR-SAGQNGAFK 384

Query: 386 IKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSKTPFSHRAEFLYK 445
           IK DYVK+PI E  F  I ++L   ++      L+PYGG M+ IS S  PF HRA  LY+
Sbjct: 385 IKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYE 444

Query: 446 ISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLDIGTNNKCGKTSY 505
           + Y   W +Q    NE+HLNWIR +Y+FMTP+VS +PR AY+NYRDLDIG N+     +Y
Sbjct: 445 LWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNY 504

Query: 506 EEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPL 533
            +A IWG KYFG NF RLV+VKT+VDP+NFFR++QSIPPL
Sbjct: 505 TQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of IVF0001661 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.4e-138
Identity = 257/536 (47.95%), Postives = 348/536 (64.93%), Query Frame = 0

Query: 11  FTSFTV-VSLSSTQWSTASSYKNKDFLRCLSIHSTDIP-----IYTPINSSYTSTLRSYA 70
           F SF + +S+++ Q         ++FL+C S +  + P     IYT  +  Y S L S  
Sbjct: 18  FLSFNIQISIANPQ---------ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTI 77

Query: 71  QNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPF 130
           QN RF T   T KPL I+ PS+VSHIQ++++CSK  GLQIR RSGGHD EGLSYIS +PF
Sbjct: 78  QNLRF-TSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPF 137

Query: 131 IVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSG 190
            +VDL N+ +++VD  + T WV++GAT+GE+YY I E +   +FP G CPTVGVGG FSG
Sbjct: 138 AIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSG 197

Query: 191 GGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVK 250
           GGYG LMR YGLAADN+IDA+LV+ +G V D ++MGE LFWAIRGGGG +FGI+  WK+K
Sbjct: 198 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIK 257

Query: 251 LVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 310
           LV VP   T   + K+++     K+  +WQ +A K +++L++  +    N T    +N T
Sbjct: 258 LVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKT 317

Query: 311 ---ASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL---- 370
                F S++LG  D L+ +MN +FP LG+ K +CKE SWI +T+  +G  N        
Sbjct: 318 TVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKK 377

Query: 371 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 430
           EILL + +     ++ IK DYVK+ I E A   I ++L   EV      L+PYGG M+ I
Sbjct: 378 EILLDR-SAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEI 437

Query: 431 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 490
           S S  PF HRA  +Y++ YT  W +Q    NE+H+NW+R +Y+F TP+VS +PR AY+NY
Sbjct: 438 SESAIPFPHRAGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNY 497

Query: 491 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPL 533
           RDLD+G  N     +Y +A IWG KYFG NF RLV+VKT  DP+NFFR++QSIPPL
Sbjct: 498 RDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of IVF0001661 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.6e-137
Identity = 257/538 (47.77%), Postives = 348/538 (64.68%), Query Frame = 0

Query: 11  FTSFTV-VSLSSTQWSTASSYKNKDFLRCLSIH-STDIP----IYTPINSSYTSTLRSYA 70
           F SF + +S+++ Q         ++FL+C S +  T++     +YT  +  Y S L S  
Sbjct: 18  FLSFNIQISIANPQ---------ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTI 77

Query: 71  QNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPF 130
           QN RF T   T KPL II P +VSHIQ T++CSK  GLQIR RSGGHD EG+SYIS +PF
Sbjct: 78  QNLRF-TSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPF 137

Query: 131 IVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSG 190
           ++VDL N+ S+++D  + T WV++GAT+GE+YY I E +  L+FPAG CPTVG GG FSG
Sbjct: 138 VIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSG 197

Query: 191 GGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVK 250
           GGYG LMR YGLAADN+IDA+LV+ +G V D ++MGE LFWAIRGGGG +FGI+  WK++
Sbjct: 198 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIR 257

Query: 251 LVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 310
           LV VP + T   + K+++     K+V +WQ +A   E+ LL+  +    N T    +N T
Sbjct: 258 LVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKT 317

Query: 311 ---ASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL---- 370
              + F S++ G  D L+ +MN +FP LG+ K +CK+ SWI + +  +G  N        
Sbjct: 318 TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKK 377

Query: 371 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 430
           EILL + +     ++ IK DYVK+PI E A   I ++L   +V       +PYGG M+ I
Sbjct: 378 EILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEI 437

Query: 431 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 490
           S S  PF HRA  +Y+I Y   W +Q    NE+H+NWIR +Y+F TP+VS +PR AY+NY
Sbjct: 438 SESAIPFPHRAGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNY 497

Query: 491 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTL 535
           RDLD+G  N     +Y +A IWG KYFG NF RLV+VKT VDP NFFR++QSIPPL L
Sbjct: 498 RDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of IVF0001661 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.6e-137
Identity = 258/538 (47.96%), Postives = 347/538 (64.50%), Query Frame = 0

Query: 11  FTSFTV-VSLSSTQWSTASSYKNKDFLRCLSIH-STDIP----IYTPINSSYTSTLRSYA 70
           F SF + +S+++ Q         ++FL+CLS +  T++     +YT  +  Y S L S  
Sbjct: 18  FLSFNIQISIANPQ---------ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTV 77

Query: 71  QNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPF 130
           QN RF T   T KPL I  P +VSHIQ T++CSK  GLQIR RSGGHD EG+SYIS +PF
Sbjct: 78  QNLRF-TSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPF 137

Query: 131 IVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSG 190
           ++VDL N+ S+++D  + T WV+SGAT+GE+YY I E +  L+FPAG CPTVG GG FSG
Sbjct: 138 VIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSG 197

Query: 191 GGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVK 250
           GGYG LMR YGLAADN+IDA+LV+ +G V D ++MGE LFWAIRGGGG +FGI+  WK++
Sbjct: 198 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIR 257

Query: 251 LVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 310
           LV VP + T   + K+++     K+V +WQ +A   E+ LL+  +    N T    +N T
Sbjct: 258 LVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKT 317

Query: 311 ---ASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL---- 370
              + F S++ G  D L+ +MN +FP LG+ K +CK+ SWI + +  +G  N        
Sbjct: 318 TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKK 377

Query: 371 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 430
           EILL + +     ++ IK DYVK+PI E A   I ++L   +V       +PYGG M+ I
Sbjct: 378 EILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEI 437

Query: 431 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 490
           S S  PF HRA   Y+I Y   W +Q    NE+H+NWIR +Y+F TP+VS +PR AY+NY
Sbjct: 438 SESAIPFPHRAGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNY 497

Query: 491 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTL 535
           RDLD+G  N     +Y +A IWG KYFG NF RLV+VKT VDP NFFR++QSIPPL L
Sbjct: 498 RDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of IVF0001661 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 535/535 (100.00%), Postives = 535/535 (100.00%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60
           MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240
           QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420
           TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 536
           IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of IVF0001661 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 535/535 (100.00%), Postives = 535/535 (100.00%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60
           MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240
           QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420
           TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 536
           IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of IVF0001661 vs. ExPASy TrEMBL
Match: A0A6J1EGP1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111434010 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.5e-218
Identity = 380/539 (70.50%), Postives = 441/539 (81.82%), Query Frame = 0

Query: 1   MKSFPLNPL-LFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP--IYTPINSSYT 60
           M +  +N L + +SFTV+      W+ A ++K++ FL+CLSIHS DI   IYTPINSSY+
Sbjct: 1   MSATVMNALFILSSFTVL------WAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYS 60

Query: 61  STLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLS 120
           S L SYA NPR S    T  P  IIKPSH+SHIQ+ V+CSKSHG QIR RSGGHD EGLS
Sbjct: 61  SLLTSYALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLS 120

Query: 121 YIS-DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTV 180
           YIS  LPFI+VDL NL SI V+  NNT WVQSGATIGE+YY IA+KSRTLAFPAGVCPTV
Sbjct: 121 YISHHLPFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTV 180

Query: 181 GVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFG 240
           G GG  SGGGYGWLMRKYGLAADNV+DAYLVDA G V D EAMGE LFWAIRGGGGGSFG
Sbjct: 181 GFGGHLSGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTK 300
           IVVGWKVKLV VP IVTTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+
Sbjct: 241 IVVGWKVKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQ 300

Query: 301 GVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 360
           G K N  A+FFSL+LG +  LI  MNTTFP LGLT  +C++TSWIQSTL+AAGFT+  PL
Sbjct: 301 GAKPNHKATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPL 360

Query: 361 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 420
           E LLTK T  N ++YKIKSDYV +PIS+HAFKGIWDRL+S ++E++QL LFPYGG+M+ I
Sbjct: 361 EFLLTK-TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKI 420

Query: 421 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 480
            + +TPFSHRAEF+YKISY VGWAEQ   A+ER+LNW+RE Y +M+PFVSNSPRAAYVNY
Sbjct: 421 PTWETPFSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNY 480

Query: 481 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 536
           RDLDIG NN+ G TSYEEA +WG  YFG+NFKRLV+VK  VDP NFFRH+QS+PPL LS
Sbjct: 481 RDLDIGMNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of IVF0001661 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 8.5e-195
Identity = 347/545 (63.67%), Postives = 425/545 (77.98%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP-----IYTPINSS 60
           MK  PL  +L  +F ++S SS+ W+ AS+ K++ FL+CLS HS D       IYTPIN S
Sbjct: 1   MKYSPLT-ILSLAFILLS-SSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPS 60

Query: 61  YTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEG 120
           Y+S L    +N RFS+  +T KPL II PS+VSH+Q+ VICSK+HGLQIRIRSGGHD EG
Sbjct: 61  YSSVLNFSVRNLRFSSA-ETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 180
           LSY++ LPFI++DLINLKSI V+  N+T W+QSGAT+GELYY IA+KSRTLAFPAGVCPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSF 240
           VGVGG FSGGGYG ++RKYGLAADNVIDAYL+DANG  +D E+MGE LFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPT 300
           GIVV WKVKLV VP IVTTC  D++LD++T  ++++WQ VA K++ENL  GI L GGN +
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNAS 300

Query: 301 K--GVKR-NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTN 360
              GVK+ NPTA+FFSL+LGK+D+L+ I+NT FP L L K +C ETSWI+STL+AA  T 
Sbjct: 301 TEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAA--TR 360

Query: 361 GQPLEIL-----LTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLF 420
            Q +E L      T  T+ N    K+KSDYVK+PI +   +GIW+RL++ ++E  QL   
Sbjct: 361 NQTIESLESLLNRTPLTIENT---KLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFV 420

Query: 421 PYGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSN 480
           PYGG+M+ IS S+TPFSHRA  LYKI Y VGW EQ   A ERHL+WIRE+Y +MTPFVS 
Sbjct: 421 PYGGRMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSK 480

Query: 481 SPRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQ 533
           SPRA YVNYRDLDIGTNNK GKTSY++AS+WG KYFGNNF RLV +K+ VDPSNFF+++Q
Sbjct: 481 SPRATYVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQ 537

BLAST of IVF0001661 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 8.5e-195
Identity = 336/537 (62.57%), Postives = 423/537 (78.77%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP-----IYTPINSS 60
           MKSFPLNPL F     + LSS+ +  AS++K++ FL+CL+ HS         IYTP N S
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  YTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEG 120
           Y+S L    QN RFST  +T KPL +I PSH+SHIQ+ VICSK+HGLQIR RSGGHD EG
Sbjct: 61  YSSILNFSIQNLRFSTA-ETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120

Query: 121 LSYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 180
           LSY+S LPFIV+DLI+L+SI +D  N+T WVQSGAT+GELYYRIA+KS+TLAFPAG CPT
Sbjct: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180

Query: 181 VGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSF 240
           VGVGG  SGGGYG+L+RKYGLAAD+VIDA+LVDANG + + E+M + LFW IRGGGGGSF
Sbjct: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240

Query: 241 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGN-- 300
           GIVV WK++LV VP  +TTC ++++LD++  K++++WQYVA+K+E+NLL+GI+LTGG+  
Sbjct: 241 GIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300

Query: 301 PTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNG 360
             +G K NP ASF + +LGK D+L+ I+NTTFP LGLTK +C ETSWI+STLI AGF+ G
Sbjct: 301 TQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAG 360

Query: 361 QPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKM 420
           + LE LL +  L  NIS KIKSDYVK+PISE  FKGIW+RL + ++E +Q+   PYGG+M
Sbjct: 361 ESLETLLYRRPLV-NISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRM 420

Query: 421 NNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAY 480
           +  S S+TP+ HRAE +YK+ Y V W EQ   A +RHLNW+RE+Y +M P+V+ SPRAAY
Sbjct: 421 SEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAY 480

Query: 481 VNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIP 531
           VNYRDLDIG NN+  KT+YE A +WG+KYFG NF RLVQVKT VDPS+FFRH+QSIP
Sbjct: 481 VNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535

BLAST of IVF0001661 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 535/535 (100.00%), Postives = 535/535 (100.00%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60
           MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240
           QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420
           TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535
           IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of IVF0001661 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 1019 bits (2635), Expect = 0.0
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60
           MK FPLNPL   SFT+VSL STQWSTASSYKNKDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180

Query: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535
           IGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 535

BLAST of IVF0001661 vs. NCBI nr
Match: XP_038895165.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 451/536 (84.14%), Postives = 490/536 (91.42%), Query Frame = 0

Query: 1   MKSFPLNPLL-FTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTST 60
           MK FPLNPLL FT+F +VS    QW+ ASSYK++DFL+CLSI+S DIPIYTPINSSY+S 
Sbjct: 1   MKVFPLNPLLTFTNFIIVSSFLVQWTAASSYKHEDFLQCLSIYSRDIPIYTPINSSYSSL 60

Query: 61  LRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYI 120
           L SYAQN RFSTQ+KTLKPL IIKPSH+SHIQ+ VICSKSHGLQIRIRSGGHDTEGLSYI
Sbjct: 61  LTSYAQNTRFSTQHKTLKPLVIIKPSHISHIQAIVICSKSHGLQIRIRSGGHDTEGLSYI 120

Query: 121 SDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVG 180
           SD+PFIV+DLINLKSIEVDT NNT WVQSGATIGELYY+IAEKSRTLAFPAGVCPTVG+G
Sbjct: 121 SDVPFIVIDLINLKSIEVDTKNNTAWVQSGATIGELYYKIAEKSRTLAFPAGVCPTVGIG 180

Query: 181 GQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVV 240
           G FSGGGYGWLMRKYGLAADNV+DAYLVD +G VFD E MGE LFWAIRGGGGGSFGIVV
Sbjct: 181 GHFSGGGYGWLMRKYGLAADNVMDAYLVDDDGRVFDRETMGEDLFWAIRGGGGGSFGIVV 240

Query: 241 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVK 300
           GWKVKLVRVPPIVTTCRL+KSLDKNTKKIVYQWQYVANKME+ LLIGINLTGGNPT+GVK
Sbjct: 241 GWKVKLVRVPPIVTTCRLEKSLDKNTKKIVYQWQYVANKMEDTLLIGINLTGGNPTQGVK 300

Query: 301 RNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEIL 360
           RNPTASFFSL+LGKT+KLI IMNTTFP LGLTK+NC+ETSWIQSTLI+AGFTNG+ LE+L
Sbjct: 301 RNPTASFFSLFLGKTNKLITIMNTTFPELGLTKSNCEETSWIQSTLISAGFTNGEALEVL 360

Query: 361 LTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSS 420
           L KP +   ISYKIKSDYVKQPIS+ A KGIW+RL+S   ETSQLFLFPYGGKM+ ISSS
Sbjct: 361 LAKPPIKT-ISYKIKSDYVKQPISQQALKGIWERLKSQVTETSQLFLFPYGGKMSKISSS 420

Query: 421 KTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDL 480
           K PFSHRAEFLYKISYTVGW EQ   ANERHLNWIRELY +MTPFVSNSPRAAY+NYRDL
Sbjct: 421 KIPFSHRAEFLYKISYTVGWTEQSIVANERHLNWIRELYDYMTPFVSNSPRAAYINYRDL 480

Query: 481 DIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535
           DIGTNNK G TSY++ASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPL+LS
Sbjct: 481 DIGTNNKYGNTSYKQASIWGLKYFGNNFKRLVQVKTKVDPSNFFRHEQSIPPLSLS 535

BLAST of IVF0001661 vs. NCBI nr
Match: XP_023520034.1 (tetrahydrocannabinolic acid synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 774 bits (1999), Expect = 1.84e-277
Identity = 380/523 (72.66%), Postives = 435/523 (83.17%), Query Frame = 0

Query: 16  VVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP--IYTPINSSYTSTLRSYAQNPRFSTQY 75
           ++S  S  W  A ++K++ FL+CLSIHS DI   IYTPINSSY+S L SYA NPR ST  
Sbjct: 11  ILSSFSVLWVVALAHKHEHFLQCLSIHSRDISKVIYTPINSSYSSLLSSYALNPRVSTP- 70

Query: 76  KTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYISD-LPFIVVDLINL 135
            T  P  IIKPSH+SHIQ+ V+CSKSHG +IR RSGGHD EGLSYIS  LPFIVVDL NL
Sbjct: 71  ATPNPRVIIKPSHLSHIQAAVVCSKSHGFRIRTRSGGHDAEGLSYISHHLPFIVVDLKNL 130

Query: 136 KSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFSGGGYGWLMR 195
            SI VD  NNT WVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GG  SGGGYGWLMR
Sbjct: 131 NSIAVDIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHLSGGGYGWLMR 190

Query: 196 KYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIV 255
           KYGLAADNV+DAYLVDA G V D EAMGE LFWAIRGGGGGSFGIVVGWKVKLV VP IV
Sbjct: 191 KYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVAVPAIV 250

Query: 256 TTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPTASFFSLYLG 315
           TTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+G K N  A+FFSL+LG
Sbjct: 251 TTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTEGAKPNHKATFFSLFLG 310

Query: 316 KTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKPTLSNNISYK 375
            + +LI  MNTTFP LGLT  +C++TSWIQSTL+AAGFTN  PLE LLTK T  N ++YK
Sbjct: 311 NSHQLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTNRDPLEFLLTK-TSFNTVAYK 370

Query: 376 IKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSKTPFSHRAEFLYK 435
           IKSDYV +PIS+HAFKGIWDRL+S ++E++QL LFPYGG+M+ I + +TPFSHRAEF+YK
Sbjct: 371 IKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSQIPTWETPFSHRAEFMYK 430

Query: 436 ISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLDIGTNNKCGKTSY 495
           ISY VGWAEQ   ANER+LNW+RE Y +M+PFVSNSPRAAYVNYRDLDIG NN+ G TSY
Sbjct: 431 ISYVVGWAEQSMEANERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIGMNNEYGSTSY 490

Query: 496 EEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535
           EEA +WG  YFG+NFKRLV+VK  VDP NFFRH+QS+PPLTLS
Sbjct: 491 EEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLTLS 531

BLAST of IVF0001661 vs. NCBI nr
Match: XP_022927076.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 765 bits (1975), Expect = 8.22e-274
Identity = 380/539 (70.50%), Postives = 441/539 (81.82%), Query Frame = 0

Query: 1   MKSFPLNPL-LFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP--IYTPINSSYT 60
           M +  +N L + +SFTV+      W+ A ++K++ FL+CLSIHS DI   IYTPINSSY+
Sbjct: 1   MSATVMNALFILSSFTVL------WAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYS 60

Query: 61  STLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLS 120
           S L SYA NPR S    T  P  IIKPSH+SHIQ+ V+CSKSHG QIR RSGGHD EGLS
Sbjct: 61  SLLTSYALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLS 120

Query: 121 YISD-LPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTV 180
           YIS  LPFI+VDL NL SI V+  NNT WVQSGATIGE+YY IA+KSRTLAFPAGVCPTV
Sbjct: 121 YISHHLPFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTV 180

Query: 181 GVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFG 240
           G GG  SGGGYGWLMRKYGLAADNV+DAYLVDA G V D EAMGE LFWAIRGGGGGSFG
Sbjct: 181 GFGGHLSGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTK 300
           IVVGWKVKLV VP IVTTC+L+K+LD   KK+VYQWQYVANKM+ENLL+G+NLTGGNPT+
Sbjct: 241 IVVGWKVKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQ 300

Query: 301 GVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 360
           G K N  A+FFSL+LG +  LI  MNTTFP LGLT  +C++TSWIQSTL+AAGFT+  PL
Sbjct: 301 GAKPNHKATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPL 360

Query: 361 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 420
           E LLTK T  N ++YKIKSDYV +PIS+HAFKGIWDRL+S ++E++QL LFPYGG+M+ I
Sbjct: 361 EFLLTK-TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKI 420

Query: 421 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 480
            + +TPFSHRAEF+YKISY VGWAEQ   A+ER+LNW+RE Y +M+PFVSNSPRAAYVNY
Sbjct: 421 PTWETPFSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNY 480

Query: 481 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535
           RDLDIG NN+ G TSYEEA +WG  YFG+NFKRLV+VK  VDP NFFRH+QS+PPL LS
Sbjct: 481 RDLDIGMNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of IVF0001661 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 3.3e-138
Identity = 253/530 (47.74%), Postives = 346/530 (65.28%), Query Frame = 0

Query: 10  LFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIP-----IYTPINSSYTSTLRSYA 69
           LFT   + SL+ +   +A    ++DFL+CLS    D       I+T  +SS+ S L S  
Sbjct: 7   LFT-ILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 66

Query: 70  QNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLP 129
           QNPRFS   +T KP++II P   S +Q+ + C++ HG+ +R RS GH  EGLSYI+ + P
Sbjct: 67  QNPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 126

Query: 130 FIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFS 189
           F V+DL NL+SI +D  N T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGGQFS
Sbjct: 127 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 186

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG L+RKYGLAADN+IDA +VDA+G + D +AMGE  FWAIRGGGG SFG+++ WKV
Sbjct: 187 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 246

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 309
           KLV VP  +T  ++ K+  K   +I+ +WQY A+K+ ++L I   L   N     K    
Sbjct: 247 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-----KNAVH 306

Query: 310 ASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKP 369
           A F  LY+G  + L+ +M   FP LGL K  C+E SWI+S L  A F  G+ L +L  + 
Sbjct: 307 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE 366

Query: 370 TLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSKTPF 429
             S  +S+K K D+V++PI E A + IW RL++ E    ++ L P+GGKM+ ++  +TPF
Sbjct: 367 RTS--LSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 426

Query: 430 SHRAEFLYKISYTVGWAEQ---GTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 489
            HR   LY+I Y   W E+        +++L W+  +Y FMTP+VS SPR AYVN++D+D
Sbjct: 427 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 486

Query: 490 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIP 531
           +G      KT YEE   WG+KYF NNF+RLV+VKT VDP++FF  +QSIP
Sbjct: 487 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of IVF0001661 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 254/531 (47.83%), Postives = 351/531 (66.10%), Query Frame = 0

Query: 10  LFTSFTVVSLSSTQWSTASSYK--NKDFLRCLS--IHSTDIP-IYTPINSSYTSTLRSYA 69
           LF  F +++LS + + + S+ +  +++FL+CLS  I+  D   I+T  + SY S L S  
Sbjct: 10  LFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSI 69

Query: 70  QNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLP 129
           QNPRF    +T KP++II P   + +QST+ C++ HG+ IR RSGGHD EGLSY++   P
Sbjct: 70  QNPRFFV-LETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRP 129

Query: 130 FIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGGQFS 189
           F+V+DL NL+SI +D  N T WVQSGATIGELYY I + S++LAFPAG+ PTVG+GGQF 
Sbjct: 130 FVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFG 189

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG LMRKYGL+ADNVIDA++VDANG   D + MGE  FWAIRGGGG SF +V+ WK+
Sbjct: 190 GGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKI 249

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKRNPT 309
           +L+ VP +VT  ++ K+ +K    I+ +WQY+A+K+  +L I   L         +    
Sbjct: 250 RLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ-------KETEVY 309

Query: 310 ASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLTKP 369
           ASF  LYLG    L+ +M   FP LGL   NC+E SWI+S L    F  G+ +EIL  + 
Sbjct: 310 ASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRK 369

Query: 370 TLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSKTPF 429
             S   S+K K D++++PI + A + +W R ++ E   +++ L P+GGKM+ I+ ++ PF
Sbjct: 370 RTSR--SFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPF 429

Query: 430 SHRAEFLYKISYTVGWAEQ---GTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 489
            HR   LY+I Y   W+E+        E++L W+  +Y FMTP+VS SPR AYVN+RD+D
Sbjct: 430 PHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDID 489

Query: 490 IGTNNKCG-KTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIP 531
           +G       KT YEEA +WG+KYF NNF RLV+VKT VDP +FF  +QSIP
Sbjct: 490 LGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of IVF0001661 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.8e-136
Identity = 259/544 (47.61%), Postives = 359/544 (65.99%), Query Frame = 0

Query: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTD------IPIYTPINS 60
           +++F L+  LF  F V  ++S    ++SS     FL C+S HS          I+ P + 
Sbjct: 4   LEAFALS--LFLVFLVKWVNS---DSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSR 63

Query: 61  SYTSTLRS-YAQNPRFST-QYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHD 120
            YT   +S  +QN RF T  + + KP+ I+ P   + IQ +++CS+  G+++R +SGGHD
Sbjct: 64  VYTDFSQSLISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHD 123

Query: 121 TEGLSYIS-DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAG 180
            EGLSY+S   PFI++DL+N++SIE++  + T WV +GATIGELYY+IA+ S+   FPAG
Sbjct: 124 YEGLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAG 183

Query: 181 VCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDS-EAMGEGLFWAIRGG 240
            CP+VGVGG FSGGG+G +MRK+GLAADNV+DA  VDANG +++S   MGE LFWAIRGG
Sbjct: 184 TCPSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGG 243

Query: 241 GGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIG--IN 300
           G  SFG+V+ WKVKLVRVP  VT  R +  L +N  KIV++WQ +A ++++NL I   ++
Sbjct: 244 GAASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVS 303

Query: 301 LTGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAA 360
           ++GG        +   +F + YLG  DKLI +MN  FP LGLT  +C E +WI S ++  
Sbjct: 304 ISGG--------SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDS-IMYF 363

Query: 361 GFTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFP 420
            +  GQPLE LL +    N++ +K KSD+VK PI E   +GIW R   HEVE+  + + P
Sbjct: 364 NWKKGQPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEP 423

Query: 421 YGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNS 480
            GGKM  I  ++TPF HR   LY I Y V W  +  G  E+H+ W+R LY +M  +VS S
Sbjct: 424 LGGKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSAS 483

Query: 481 PRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQS 533
           PR AY+NYRDLD+G N     TS+E+A +WG +YFG+NFKRL  VK  +DP+NFFR++QS
Sbjct: 484 PRGAYLNYRDLDLGMNRGV-NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQS 530

BLAST of IVF0001661 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 481.5 bits (1238), Expect = 8.9e-136
Identity = 251/537 (46.74%), Postives = 350/537 (65.18%), Query Frame = 0

Query: 2   KSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDI-----PIYTPINSSY 61
           +SF  + L+F + + + LS     +A+      FL+CLS+   D       I+TP ++S+
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  TSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGL 121
           +S L S  QN RFS      KP+ I+ P   S +QS V C++  G+ IR RSGGHD EGL
Sbjct: 64  SSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123

Query: 122 SYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTV 181
           SY++  PF+++DL NL+SI VD  N +VWVQ+GATIGELYY I +K+RTLAFPAGVCPTV
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 183

Query: 182 GVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFG 241
           GVGG FSGGGYG L+RK+GLAAD+VIDA +VDA G + +   MGE  FWAIRGGGG SF 
Sbjct: 184 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 243

Query: 242 IVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTK 301
           +V+ WK+ L+ VP  VT   + K  +++  KI+++WQ+VA+K+ ++L I + L      +
Sbjct: 244 VVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML------Q 303

Query: 302 GVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 361
             K    ASF  LYLG    L+K++N  FP LGL + +C E SWI+S +  A     +P+
Sbjct: 304 RYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPI 363

Query: 362 EILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNI 421
            + LTK T + ++++K KSD+V++P+ + A   +W RLQ  E E +QL   P+GGKM+ I
Sbjct: 364 NV-LTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 423

Query: 422 SSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNY 481
           +  +TPF HR   +Y+I Y   W        E+++ W+  +Y  M+ FV+ SPR AY+N 
Sbjct: 424 ADYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINL 483

Query: 482 RDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLT 534
           RDLD+G      ++ YEE   WG+KYF NNF+RLV+VKT VDPS+FF  +QSIPP T
Sbjct: 484 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of IVF0001661 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 475.7 bits (1223), Expect = 4.9e-134
Identity = 248/548 (45.26%), Postives = 347/548 (63.32%), Query Frame = 0

Query: 2   KSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPIN-------- 61
           K  PL  +L   F++ +++ T  S+ +S +++ F+ C+     +  +Y P+         
Sbjct: 5   KPLPLFSILVLYFSLYTITPT--SSLASLQDQ-FINCV---QRNTHVYFPLEKTFFAPTK 64

Query: 62  --SSYTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGH 121
             S ++  L S AQN RF  +    KP  I  P H SH+Q+++ICSK   + +R+RSGGH
Sbjct: 65  NVSMFSQVLESTAQNLRF-LKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGH 124

Query: 122 DTEGLSYIS--DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFP 181
           D EGLSY+S  D PFI++DL  ++ + ++  +N+ WVQSGAT+GELYYRIAEKS+   FP
Sbjct: 125 DYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFP 184

Query: 182 AGVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRG 241
           AG+C ++G+GG  +GG YG +MRKYGL ADNV+DA +VDANG + D  AMGE  FWAIRG
Sbjct: 185 AGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRG 244

Query: 242 GGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNT-KKIVYQWQYVANKMEENLLIGIN 301
           G GGSFGI++ WK+KLV VP  VT   + K+L ++   KI+ +WQ VA+K+ E L I + 
Sbjct: 245 GAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVL 304

Query: 302 L----TGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQST 361
                TGGN T       T S+ +L+LG    L+ +M  +FP LGLT  +C E SW++S 
Sbjct: 305 FNVAGTGGNKT------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESI 364

Query: 362 LIAAGFTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQL 421
              +GF    P  +LL   +    +S+K KSD+VK PI E   +GI+ +L   ++    +
Sbjct: 365 AYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--M 424

Query: 422 FLFPYGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPF 481
              PYGG M  I  S+ PF HR   L+K+ Y   W +     + RH+NWIR+LYS+MTP+
Sbjct: 425 IWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPS-RHINWIRDLYSYMTPY 484

Query: 482 VSNSPRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFR 533
           VS++PR AYVNYRDLD+G N K  KT  ++A +WG  YF NNF RL+ +K  VDP NFFR
Sbjct: 485 VSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFR 536