IVF0001357 (gene) Melon (IVF77) v1

Overview
NameIVF0001357
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description5'-nucleotidase
Locationchr10: 942936 .. 945186 (+)
RNA-Seq ExpressionIVF0001357
SyntenyIVF0001357
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCCATACCAAAAAAGGAGGAGTGTTCATTAGATTTCAGGCGAACGCCCAAATGACTGTTCGAGTCCAATTCATTTCAACCACTCCTCTTCGTTTCGCCTCCATCTTCAATCTCCGTTCTTCTCCATTTTCCACTTCCAGGTTTCTTCTCTCTTCAACTCGATTCCATTGTTTTTGCCCTTCAATACATCGCGGATTCGTTTGCACGATTATGCCGCATTCTTCCTTCCCTTATCGTTTCTCGTGTTCCTGATCTTTACTAATGCGTTTTTCTTTTGCAGGCTCTTGTTTTGCGGACGCCGAAACGTGATTGGAATGGACAAAGAAGGATTCTTACAGCCTGCGGAGGCAGTTGTCGCCGATTCCGATTTGCTGAAGAAGAAACTTGATGCTTTGCGTTTTTCCGGTCCGCAGAAGCTCCAGGTGTGTTGCGCTTTTGTTTTTGAGTCTGACGTTTCTACGAGTCGTGATTTTTTCTCTTTACCATTAGGAATAATAGTAGGAGGTCTTGTTTAGGTTATTGCCGATTTTGATGCTACGCTAACGAGGTACTGGATTGATGGCTGCCGTGGTCAAAGTAAGATTTGATGATTTTCAACTTGTCAACTTTGTAGTAAGCTTTGTTTTTAAGTCTGATGAAGTTGATTAATATGTGTAGCTAGTCATGGTCTTCTAAAGCAGGGGAATCCTGAATATGATACTAAGAGGGATGAACTTTATAAATATTACCATCCTTTGGAATATTCTCCTACAATTTCGATAGAAGAGAAAACCAAGCTCATGGAAGAATGGTATGTCTCGTTCGTATCATCTCTTAGATTTAGCTAAAAAATATGTATGTAGTACCAGTATAAGTTTAAGCTGAGTTATTTTCTGATGCTTCAATCGTACATGATGGATTTATGTCCACTTGTTGGTTCTGGTTGGTGGGTCTACAAAAATTTATAATTTCATATCATGCATTGCAGGTGGGGCAAAAGCCATTCACTTCTGATTGAAGGAGGTCTTACGTTTGATGCAATAAAGCAATCAGTTGCTGCTGCCACTATTGCTTTTAGGGAGGGAGTGGTTGAGCTGTTTGAACTTCTAGAGGTAAAAAAATTTTCCCCCTGAAATGATAACTTTACTTGCTACTCTCCTTTGTGGGGGTAAATATGAATGTTCTATTTTCCATTTTTGTGATTTTGTATTGAAGCTAATTTTTCATATCAAACACAATGATGATCAGGAAAAGGGTGTTCCTGTTCTTATATTTTCAGCAGGCCTTGCTGACATTATAGAGGAGGTAGGCAAGGTCAAATGTTCCTTGATCTTTTATGCATTTATACAGATACAATTCAATATGTAAAGCTCACTTTCATTCCTTCTTGATATTTCCTTTTTCTTCTTACTGGACTAGGTCCTGAGGCAGAAACTTCACAGATCTTTCAAAAATATTAGGATCGTTTCAAATAGAATGGTGTTTGATGATAATGGTTGCCTTGTATCATTTAAGGGTATTTTCCTCTTTTTAGCTGGCTATTACTTGCCACGGTCATTTGATTAGCGGTTATTATGATTGTTACATCTGATAGTCTGAATGAGTTTGTTCTGTGGAAATATCTCTTTGCAGGCAAGACGATTCATAGTCTAAATAAAAATGAGCATGCTCTTGATATGGCTGCTCCTCTGCATGACCAATTGGGTGATGTAGATGGCGCAATTAATGACAGCGCTTCTGTAAGAAAACGGACAAATGTACTTCTTCTTGGTGACCACATTGGGGATTTGGGGATGTCAGACGGTTTAAACTATGACACTCGGATATCCGTGGGATTTTTGTATGTGAACTTCTTGATAACTTTCATATGTTGTCATCCGTTTACTTGTTCTGCATGGAAAATATTCGTAGAAAAATAGTTTTGTTGTAAGACCGCTTTAAGGTACTGTTTGTGTCACGATGAATATTTATTGTCATACTGAAGAATAAGCTCCTTTTGCAGGAATGACAACATCGAGAACTCTCTTGATAGCTATCGTAATGCCTTTGATATTGTTTACCTGGTGAGTCGAACACCCTTAAATAGGAAGAAAAAGATAGGAAAATGTTTACTTTATTTTCTCATGACCAATATTGCTAATACCATACTTCTCCCCGTATAATGAGAACCACTGTACCAGAAAGCTTTAACCAATTTGAATGTTGTTTGCAGAATGATGCATCCATGCTGGGAGTGGTTAAACTTGTACGTCAGCTCTTTCCCGTTTGA

mRNA sequence

ATGCCCCATACCAAAAAAGGAGGAGTGTTCATTAGATTTCAGGCGAACGCCCAAATGACTGTTCGAGTCCAATTCATTTCAACCACTCCTCTTCGTTTCGCCTCCATCTTCAATCTCCGTTCTTCTCCATTTTCCACTTCCAGGCTCTTGTTTTGCGGACGCCGAAACGTGATTGGAATGGACAAAGAAGGATTCTTACAGCCTGCGGAGGCAGTTGTCGCCGATTCCGATTTGCTGAAGAAGAAACTTGATGCTTTGCGTTTTTCCGGTCCGCAGAAGCTCCAGGTTATTGCCGATTTTGATGCTACGCTAACGAGGTACTGGATTGATGGCTGCCGTGGTCAAACTAGTCATGGTCTTCTAAAGCAGGGGAATCCTGAATATGATACTAAGAGGGATGAACTTTATAAATATTACCATCCTTTGGAATATTCTCCTACAATTTCGATAGAAGAGAAAACCAAGCTCATGGAAGAATGGTGGGGCAAAAGCCATTCACTTCTGATTGAAGGAGGTCTTACGTTTGATGCAATAAAGCAATCAGTTGCTGCTGCCACTATTGCTTTTAGGGAGGGAGTGGTTGAGCTGTTTGAACTTCTAGAGGAAAAGGGTGTTCCTGTTCTTATATTTTCAGCAGGCCTTGCTGACATTATAGAGGAGGTCCTGAGGCAGAAACTTCACAGATCTTTCAAAAATATTAGGATCGTTTCAAATAGAATGGTGTTTGATGATAATGGTTGCCTTGTATCATTTAAGGGCAAGACGATTCATAGTCTAAATAAAAATGAGCATGCTCTTGATATGGCTGCTCCTCTGCATGACCAATTGGGTGATGTAGATGGCGCAATTAATGACAGCGCTTCTGTAAGAAAACGGACAAATGTACTTCTTCTTGGTGACCACATTGGGGATTTGGGGATGTCAGACGGTTTAAACTATGACACTCGGATATCCGTGGGATTTTTGAATGACAACATCGAGAACTCTCTTGATAGCTATCGTAATGCCTTTGATATTGTTTACCTGAATGATGCATCCATGCTGGGAGTGGTTAAACTTGTACGTCAGCTCTTTCCCGTTTGA

Coding sequence (CDS)

ATGCCCCATACCAAAAAAGGAGGAGTGTTCATTAGATTTCAGGCGAACGCCCAAATGACTGTTCGAGTCCAATTCATTTCAACCACTCCTCTTCGTTTCGCCTCCATCTTCAATCTCCGTTCTTCTCCATTTTCCACTTCCAGGCTCTTGTTTTGCGGACGCCGAAACGTGATTGGAATGGACAAAGAAGGATTCTTACAGCCTGCGGAGGCAGTTGTCGCCGATTCCGATTTGCTGAAGAAGAAACTTGATGCTTTGCGTTTTTCCGGTCCGCAGAAGCTCCAGGTTATTGCCGATTTTGATGCTACGCTAACGAGGTACTGGATTGATGGCTGCCGTGGTCAAACTAGTCATGGTCTTCTAAAGCAGGGGAATCCTGAATATGATACTAAGAGGGATGAACTTTATAAATATTACCATCCTTTGGAATATTCTCCTACAATTTCGATAGAAGAGAAAACCAAGCTCATGGAAGAATGGTGGGGCAAAAGCCATTCACTTCTGATTGAAGGAGGTCTTACGTTTGATGCAATAAAGCAATCAGTTGCTGCTGCCACTATTGCTTTTAGGGAGGGAGTGGTTGAGCTGTTTGAACTTCTAGAGGAAAAGGGTGTTCCTGTTCTTATATTTTCAGCAGGCCTTGCTGACATTATAGAGGAGGTCCTGAGGCAGAAACTTCACAGATCTTTCAAAAATATTAGGATCGTTTCAAATAGAATGGTGTTTGATGATAATGGTTGCCTTGTATCATTTAAGGGCAAGACGATTCATAGTCTAAATAAAAATGAGCATGCTCTTGATATGGCTGCTCCTCTGCATGACCAATTGGGTGATGTAGATGGCGCAATTAATGACAGCGCTTCTGTAAGAAAACGGACAAATGTACTTCTTCTTGGTGACCACATTGGGGATTTGGGGATGTCAGACGGTTTAAACTATGACACTCGGATATCCGTGGGATTTTTGAATGACAACATCGAGAACTCTCTTGATAGCTATCGTAATGCCTTTGATATTGTTTACCTGAATGATGCATCCATGCTGGGAGTGGTTAAACTTGTACGTCAGCTCTTTCCCGTTTGA

Protein sequence

MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV
Homology
BLAST of IVF0001357 vs. ExPASy Swiss-Prot
Match: Q7ZWS2 (7-methylguanosine phosphate-specific 5'-nucleotidase A OS=Xenopus laevis OX=8355 GN=Nt5c3b-a PE=2 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-45
Identity = 105/279 (37.63%), Postives = 155/279 (55.56%), Query Frame = 0

Query: 75  DSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDE 134
           D + L+ K+  ++  G +KLQ+I+DFD TL+R+  +G R  T + ++   N   D  R +
Sbjct: 15  DPEGLQDKITRIQRGGQEKLQIISDFDMTLSRFSRNGERCPTCYNIIDNSNIISDEGRKK 74

Query: 135 ---LYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFRE 194
              L+  Y+PLE  P  SIEEK  LM EWW K+H L  E  +  D + Q V  +    R+
Sbjct: 75  LKCLFDIYYPLEIDPKKSIEEKYPLMVEWWSKAHDLFYEQRIQKDRLAQVVKESQATLRD 134

Query: 195 GVVELFELLEEKGVPVLIFSAGLADIIEEVLRQK--LHRSFKNIRIVSNRMVFDDNGCLV 254
           G    F  L ++ +P+ IFSAG+ D++EE++RQ    H    N ++VSN M FDDNG L 
Sbjct: 135 GYDLFFNSLYQREIPLFIFSAGIGDVLEEIIRQAGVFH---PNTKVVSNYMDFDDNGILT 194

Query: 255 SFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSD 314
            FKG  IH+ NKN   L       +              +  RTN+LLLGD +GDL M+D
Sbjct: 195 GFKGDLIHTYNKNSSVLKDTEYFKE--------------ISHRTNILLLGDTLGDLTMAD 254

Query: 315 GLN-YDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASM 348
           G++  +  I +GFLND +E   + +  ++DIV L D ++
Sbjct: 255 GVSTVENIIKIGFLNDKVEELTEQFLQSYDIVLLRDETL 276

BLAST of IVF0001357 vs. ExPASy Swiss-Prot
Match: Q2TAG6 (7-methylguanosine phosphate-specific 5'-nucleotidase B OS=Xenopus laevis OX=8355 GN=Nt5c3b-b PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.7e-45
Identity = 105/279 (37.63%), Postives = 154/279 (55.20%), Query Frame = 0

Query: 75  DSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDE 134
           D + L+ K+  ++  G +KLQ+I+DFD TL+R+  +G R  T + ++   N   D  R +
Sbjct: 15  DPEGLQDKITRIQRGGQEKLQIISDFDMTLSRFSRNGERCPTCYNIIDNSNIISDEGRKK 74

Query: 135 ---LYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFRE 194
              L+  Y+PLE  P  SIEEK  LM EWW K+H L  E  +  D + Q V       R+
Sbjct: 75  LKCLFDIYYPLEIDPKKSIEEKYPLMVEWWSKAHDLFYEQRIQKDRLAQVVKEPQATLRD 134

Query: 195 GVVELFELLEEKGVPVLIFSAGLADIIEEVLRQK--LHRSFKNIRIVSNRMVFDDNGCLV 254
           G    F  L ++ +P+ IFSAG+ D++EE++RQ    H    N ++VSN M FDDNG L 
Sbjct: 135 GYDLFFNNLYQREIPLFIFSAGIGDVLEEIIRQAGVFH---PNTKVVSNYMDFDDNGILT 194

Query: 255 SFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSD 314
            FKG  IH+ NKN   L       +              +  RTN+LLLGD +GDL M+D
Sbjct: 195 GFKGDLIHTYNKNSSVLKDTEYFKE--------------ISHRTNILLLGDTLGDLTMAD 254

Query: 315 GLN-YDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASM 348
           G++  +  I +GFLND +E   + +  ++DIV L D ++
Sbjct: 255 GVSTVENIIKIGFLNDKVEELTEQFLQSYDIVLLRDETL 276

BLAST of IVF0001357 vs. ExPASy Swiss-Prot
Match: Q5ZKF6 (7-methylguanosine phosphate-specific 5'-nucleotidase OS=Gallus gallus OX=9031 GN=NT5C3B PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-44
Identity = 104/296 (35.14%), Postives = 165/296 (55.74%), Query Frame = 0

Query: 66  LQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQG- 125
           LQ +   +   + +   + A++  G  KLQVI+DFD TL+R+  +G R  TSH +L    
Sbjct: 5   LQKSTVRIQQPERVMGVIRAIKEQGCSKLQVISDFDMTLSRFGCNGRRCPTSHNILDNSH 64

Query: 126 --NPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSV 185
             + +   K  +L  +Y+P+E  P  ++EEK  LM EWW ++H LL +  +    I Q V
Sbjct: 65  VISEDGKKKLKDLLHHYYPIEIDPNRTLEEKRPLMVEWWTRAHELLSQQKIQKGDIAQIV 124

Query: 186 AAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVF 245
             + +  R+G  ELF+ L +  VP+ IFSAG+ D++EE++RQ  +  + N+ +VSN M F
Sbjct: 125 RESDVMLRDGFNELFDQLHKYSVPMFIFSAGVGDVLEEIIRQ-ANVFYPNVNVVSNYMDF 184

Query: 246 DDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHI 305
           DD+G L  FK   IH+ NKN   L   A                  +  RT+++LLGD +
Sbjct: 185 DDSGVLKCFKSPLIHTYNKNNSVLQGTAYFQ--------------QLSTRTSIILLGDSM 244

Query: 306 GDLGMSDGL-NYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQL 358
           GDL M+DG+ + +  + +GFLND +E     Y +A+DIV  +D ++  V  ++R +
Sbjct: 245 GDLTMADGVPSVENILKIGFLNDKVEEQRGRYLDAYDIVLESDETLDVVNGILRYI 285

BLAST of IVF0001357 vs. ExPASy Swiss-Prot
Match: Q7SYN4 (Cytosolic 5'-nucleotidase 3 OS=Danio rerio OX=7955 GN=nt5c3 PE=2 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-44
Identity = 99/282 (35.11%), Postives = 164/282 (58.16%), Query Frame = 0

Query: 73  VADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKR 132
           + D + +++ +  L   G  KLQ+I DFD TL+R+ ++G R  + H ++       D  R
Sbjct: 12  IRDPERVEQIICGLIKGGASKLQIITDFDMTLSRFAVNGKRCPSCHNIIDNSKLVTDDCR 71

Query: 133 DELY---KYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAF 192
            +L    + Y+P+E  P +++EEK   M EW+ KSH+LL+E  L  D + ++V  + ++ 
Sbjct: 72  KKLVHLKETYYPIEIDPHLTMEEKYPFMVEWYFKSHTLLVEQRLEKDKLPEAVRESDVSL 131

Query: 193 REGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLV 252
           +EG  + F+ L +  VPV IFSAGL D++EE++RQ       N+++VSN M FDDNG L 
Sbjct: 132 KEGYEQFFDRLHQHSVPVFIFSAGLGDVLEEIIRQ-AGVYHPNVKVVSNFMDFDDNGVLK 191

Query: 253 SFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDS---ASVRKRTNVLLLGDHIGDLG 312
            FKG+ IH  NK+                 DGA+ ++     ++   N++LLGD +GDL 
Sbjct: 192 GFKGELIHVYNKH-----------------DGALRNTEYFKQLKDNGNIILLGDSLGDLT 251

Query: 313 MSDGL-NYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASM 348
           M+DG+ N +  + +G+LND +E  L+ Y ++++IV   D ++
Sbjct: 252 MADGVPNVENILKIGYLNDKVEELLEKYMDSYNIVLARDETL 275

BLAST of IVF0001357 vs. ExPASy Swiss-Prot
Match: Q3UFY7 (7-methylguanosine phosphate-specific 5'-nucleotidase OS=Mus musculus OX=10090 GN=Nt5c3b PE=1 SV=3)

HSP 1 Score: 180.6 bits (457), Expect = 3.1e-44
Identity = 104/296 (35.14%), Postives = 165/296 (55.74%), Query Frame = 0

Query: 66  LQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQG- 125
           L  A  ++     +++ + ALR  G  +LQVI+DFD TL+R+  +G R  +SH +L    
Sbjct: 8   LMKATVLMRQPGRVQEIVGALRRGGGDRLQVISDFDMTLSRFAYNGQRCPSSHNILDNSK 67

Query: 126 --NPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSV 185
             + +   +  EL+ +Y+P+E  P  +I+EK   M +WW K+HSLL +  +    I Q V
Sbjct: 68  IISEDCRKELTELFHHYYPIEIDPHRTIKEKLPHMVQWWSKAHSLLCQQRIQKVQIAQVV 127

Query: 186 AAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVF 245
             +T   REG    F+ L +  +P+ IFSAG+ DI+EE++RQ +     NI IVSN M F
Sbjct: 128 GESTAMLREGYKTFFDTLYQNNIPLFIFSAGIGDILEEIIRQ-MKVFHPNIHIVSNYMDF 187

Query: 246 DDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHI 305
            ++G L  FKG+ IH+ NKN    + ++                  ++ +TN++LLGD I
Sbjct: 188 SEDGFLKGFKGQLIHTYNKNSSVCENSSYFQ--------------QLQNKTNIILLGDSI 247

Query: 306 GDLGMSDGL-NYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQL 358
           GDL M+DG+      + +GFLND +E   + Y +++DIV   D ++  V  L+R +
Sbjct: 248 GDLTMADGVPGVQNILKIGFLNDKVEERRERYMDSYDIVLEKDETLDVVNGLLRHI 288

BLAST of IVF0001357 vs. ExPASy TrEMBL
Match: A0A1S3BGK8 (5'-nucleotidase OS=Cucumis melo OX=3656 GN=LOC103489365 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 6.8e-204
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60
           MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM
Sbjct: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60

Query: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120
           DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL
Sbjct: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120

Query: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180
           LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ
Sbjct: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180

Query: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240
           SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM
Sbjct: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240

Query: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300
           VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD
Sbjct: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300

Query: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV
Sbjct: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360

BLAST of IVF0001357 vs. ExPASy TrEMBL
Match: A0A5A7SXP5 (5'-nucleotidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001800 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 6.8e-204
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60
           MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM
Sbjct: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60

Query: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120
           DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL
Sbjct: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120

Query: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180
           LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ
Sbjct: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180

Query: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240
           SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM
Sbjct: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240

Query: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300
           VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD
Sbjct: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300

Query: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV
Sbjct: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360

BLAST of IVF0001357 vs. ExPASy TrEMBL
Match: A0A0A0KRD5 (5'-nucleotidase OS=Cucumis sativus OX=3659 GN=Csa_5G613460 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 332/344 (96.51%), Postives = 335/344 (97.38%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIF--NLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADS 78
           MTVRVQFISTTPLRFASIF  NLRSSPFSTSR LFC RRNVIGMD++GFLQP E VVADS
Sbjct: 1   MTVRVQFISTTPLRFASIFNLNLRSSPFSTSR-LFCERRNVIGMDEKGFLQPTETVVADS 60

Query: 79  DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY 138
           DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY
Sbjct: 61  DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY 120

Query: 139 KYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVEL 198
           KYYHPLEYSPTIS EEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSV AATIAFREGVVEL
Sbjct: 121 KYYHPLEYSPTISREEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVTAATIAFREGVVEL 180

Query: 199 FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI 258
           FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI
Sbjct: 181 FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI 240

Query: 259 HSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR 318
           HSLNKNEHALDMAAPLHDQLGDVDG INDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR
Sbjct: 241 HSLNKNEHALDMAAPLHDQLGDVDGTINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR 300

Query: 319 ISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 361
           ISVGFLNDNIENSLDSYRNAFD+VYLNDASMLGVVKLVRQLFPV
Sbjct: 301 ISVGFLNDNIENSLDSYRNAFDVVYLNDASMLGVVKLVRQLFPV 343

BLAST of IVF0001357 vs. ExPASy TrEMBL
Match: A0A6J1J014 (5'-nucleotidase OS=Cucurbita maxima OX=3661 GN=LOC111481459 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.3e-170
Identity = 306/342 (89.47%), Postives = 319/342 (93.27%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDL 78
           M+ RV  IST P RF SI  L S P S SRL FCG  NVIGM+++GFLQ +EAVVAD+DL
Sbjct: 1   MSGRVLCISTIPFRFHSISKLPSYPSSNSRLWFCGNPNVIGMEEQGFLQASEAVVADTDL 60

Query: 79  LKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKY 138
           LKKKL+A+R SGPQKLQVIADFDATLTRYWIDG RGQ+SHGLLKQGNP+YD KR+ELYKY
Sbjct: 61  LKKKLEAMRLSGPQKLQVIADFDATLTRYWIDGRRGQSSHGLLKQGNPDYDDKREELYKY 120

Query: 139 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 198
           YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVA ATIAFREGVVELFE
Sbjct: 121 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVADATIAFREGVVELFE 180

Query: 199 LLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 258
           LLEE+ VPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS
Sbjct: 181 LLEERNVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 240

Query: 259 LNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 318
           LNKNEHALDMAAPLHDQLGDV+GA NDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS
Sbjct: 241 LNKNEHALDMAAPLHDQLGDVNGATNDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 300

Query: 319 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 361
           VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLV QLFPV
Sbjct: 301 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVHQLFPV 342

BLAST of IVF0001357 vs. ExPASy TrEMBL
Match: A0A6J1D8T4 (5'-nucleotidase OS=Momordica charantia OX=3673 GN=LOC111018671 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 302/340 (88.82%), Postives = 321/340 (94.41%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDL 78
           M+ RVQ +ST PLRF SI NLRSSP   SRL FCG RN IGM+++GFL+ +EAVVADSDL
Sbjct: 1   MSGRVQCLSTVPLRFNSISNLRSSPSLNSRLWFCGSRNAIGMEEKGFLKDSEAVVADSDL 60

Query: 79  LKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKY 138
           LKKKLDA+R SGP KLQVIADFDATLTRYWIDG RGQTSHGLLKQGNPEYD KRD+LYKY
Sbjct: 61  LKKKLDAMRGSGPHKLQVIADFDATLTRYWIDGRRGQTSHGLLKQGNPEYDAKRDDLYKY 120

Query: 139 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 198
           YHP+EYSPTIS+EEKTKLME+WWGK+HSLLIEGGLTFDAIKQSV+AATIAFREGVVELFE
Sbjct: 121 YHPIEYSPTISVEEKTKLMEDWWGKTHSLLIEGGLTFDAIKQSVSAATIAFREGVVELFE 180

Query: 199 LLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 258
           LLEEK VPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS
Sbjct: 181 LLEEKDVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 240

Query: 259 LNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 318
           LNKNEHALDMAAPLHDQLGDVDGAI+D+ASVRKR NVLLLGDHIGDLGMSDGLNYDTRIS
Sbjct: 241 LNKNEHALDMAAPLHDQLGDVDGAIDDTASVRKRKNVLLLGDHIGDLGMSDGLNYDTRIS 300

Query: 319 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLF 359
           VGFLNDNIE SL+SYRNAFDIVYLNDASM+GVVKLV+QLF
Sbjct: 301 VGFLNDNIEKSLESYRNAFDIVYLNDASMMGVVKLVQQLF 340

BLAST of IVF0001357 vs. NCBI nr
Match: XP_008446729.1 (PREDICTED: 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis melo] >XP_008446730.1 PREDICTED: 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis melo] >KAA0034636.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis melo var. makuwa] >TYK09188.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis melo var. makuwa])

HSP 1 Score: 717 bits (1851), Expect = 1.06e-260
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60
           MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM
Sbjct: 1   MPHTKKGGVFIRFQANAQMTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGM 60

Query: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120
           DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL
Sbjct: 61  DKEGFLQPAEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGL 120

Query: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180
           LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ
Sbjct: 121 LKQGNPEYDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQ 180

Query: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240
           SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM
Sbjct: 181 SVAAATIAFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRM 240

Query: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300
           VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD
Sbjct: 241 VFDDNGCLVSFKGKTIHSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGD 300

Query: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV
Sbjct: 301 HIGDLGMSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360

BLAST of IVF0001357 vs. NCBI nr
Match: XP_004135048.1 (7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis sativus] >XP_011655825.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis sativus] >XP_031741960.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis sativus] >XP_031741961.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis sativus] >KGN52168.1 hypothetical protein Csa_009007 [Cucumis sativus])

HSP 1 Score: 654 bits (1686), Expect = 7.86e-236
Identity = 332/344 (96.51%), Postives = 335/344 (97.38%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNL--RSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADS 78
           MTVRVQFISTTPLRFASIFNL  RSSPFSTSRL FC RRNVIGMD++GFLQP E VVADS
Sbjct: 1   MTVRVQFISTTPLRFASIFNLNLRSSPFSTSRL-FCERRNVIGMDEKGFLQPTETVVADS 60

Query: 79  DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY 138
           DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY
Sbjct: 61  DLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELY 120

Query: 139 KYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVEL 198
           KYYHPLEYSPTIS EEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSV AATIAFREGVVEL
Sbjct: 121 KYYHPLEYSPTISREEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVTAATIAFREGVVEL 180

Query: 199 FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI 258
           FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI
Sbjct: 181 FELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTI 240

Query: 259 HSLNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR 318
           HSLNKNEHALDMAAPLHDQLGDVDG INDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR
Sbjct: 241 HSLNKNEHALDMAAPLHDQLGDVDGTINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTR 300

Query: 319 ISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           ISVGFLNDNIENSLDSYRNAFD+VYLNDASMLGVVKLVRQLFPV
Sbjct: 301 ISVGFLNDNIENSLDSYRNAFDVVYLNDASMLGVVKLVRQLFPV 343

BLAST of IVF0001357 vs. NCBI nr
Match: XP_038893141.1 (7-methylguanosine phosphate-specific 5'-nucleotidase A [Benincasa hispida] >XP_038893142.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Benincasa hispida] >XP_038893143.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Benincasa hispida] >XP_038893144.1 7-methylguanosine phosphate-specific 5'-nucleotidase A [Benincasa hispida])

HSP 1 Score: 618 bits (1593), Expect = 1.26e-221
Identity = 310/342 (90.64%), Postives = 324/342 (94.74%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDL 78
           M+V VQFIST PLRF SI NLRSSPFSTSRL F G RN+IGM+++GFL  +E V+ADS L
Sbjct: 1   MSVVVQFISTIPLRFTSISNLRSSPFSTSRLWFSGCRNLIGMEEKGFLDASEEVIADSHL 60

Query: 79  LKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKY 138
           LKKKLDA+RF+GPQKLQVIADFDATLTRYWIDG RGQTSHGLLKQGN EYD KRDELYKY
Sbjct: 61  LKKKLDAMRFAGPQKLQVIADFDATLTRYWIDGRRGQTSHGLLKQGNSEYDAKRDELYKY 120

Query: 139 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 198
           YHPLEYSPTI IEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE
Sbjct: 121 YHPLEYSPTILIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 180

Query: 199 LLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 258
           LLEE+ VPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS
Sbjct: 181 LLEERDVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 240

Query: 259 LNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 318
           LNKNEHALDMAAPLHDQLGD+DGAI+DSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS
Sbjct: 241 LNKNEHALDMAAPLHDQLGDIDGAIDDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 300

Query: 319 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           VGFLNDN+ENSLD YRNAFDIVYLNDASM+GVVKLV QLFPV
Sbjct: 301 VGFLNDNVENSLDGYRNAFDIVYLNDASMMGVVKLVHQLFPV 342

BLAST of IVF0001357 vs. NCBI nr
Match: KAG6601237.1 (Cytosolic 5'-nucleotidase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032028.1 Cytosolic 5'-nucleotidase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605 bits (1561), Expect = 9.41e-217
Identity = 306/342 (89.47%), Postives = 320/342 (93.57%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDL 78
           M+ RV  IST P RF SI  L S P S SRL FCG  NVIGM+++GFLQ +EAVVAD+DL
Sbjct: 1   MSGRVLCISTIPFRFHSISKLPSYPSSNSRLWFCGNPNVIGMEEQGFLQASEAVVADTDL 60

Query: 79  LKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKY 138
           LKKKL+A+R SGPQKLQVIADFDATLTRYWIDG RGQ+SHGLLKQGNP+YD KR+ELYKY
Sbjct: 61  LKKKLEAMRLSGPQKLQVIADFDATLTRYWIDGRRGQSSHGLLKQGNPDYDDKREELYKY 120

Query: 139 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 198
           YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVA+ATIAFREGVVELFE
Sbjct: 121 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVASATIAFREGVVELFE 180

Query: 199 LLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 258
           LLEE+ VPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS
Sbjct: 181 LLEERDVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 240

Query: 259 LNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 318
           LNKNEHALDMAAPLHDQLGDV+GA NDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS
Sbjct: 241 LNKNEHALDMAAPLHDQLGDVNGATNDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 300

Query: 319 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLV QLFPV
Sbjct: 301 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVHQLFPV 342

BLAST of IVF0001357 vs. NCBI nr
Match: XP_022982686.1 (7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucurbita maxima])

HSP 1 Score: 605 bits (1559), Expect = 1.90e-216
Identity = 306/342 (89.47%), Postives = 319/342 (93.27%), Query Frame = 0

Query: 19  MTVRVQFISTTPLRFASIFNLRSSPFSTSRLLFCGRRNVIGMDKEGFLQPAEAVVADSDL 78
           M+ RV  IST P RF SI  L S P S SRL FCG  NVIGM+++GFLQ +EAVVAD+DL
Sbjct: 1   MSGRVLCISTIPFRFHSISKLPSYPSSNSRLWFCGNPNVIGMEEQGFLQASEAVVADTDL 60

Query: 79  LKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRGQTSHGLLKQGNPEYDTKRDELYKY 138
           LKKKL+A+R SGPQKLQVIADFDATLTRYWIDG RGQ+SHGLLKQGNP+YD KR+ELYKY
Sbjct: 61  LKKKLEAMRLSGPQKLQVIADFDATLTRYWIDGRRGQSSHGLLKQGNPDYDDKREELYKY 120

Query: 139 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATIAFREGVVELFE 198
           YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVA ATIAFREGVVELFE
Sbjct: 121 YHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVADATIAFREGVVELFE 180

Query: 199 LLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 258
           LLEE+ VPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS
Sbjct: 181 LLEERNVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGCLVSFKGKTIHS 240

Query: 259 LNKNEHALDMAAPLHDQLGDVDGAINDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 318
           LNKNEHALDMAAPLHDQLGDV+GA NDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS
Sbjct: 241 LNKNEHALDMAAPLHDQLGDVNGATNDSASVRKRTNVLLLGDHIGDLGMSDGLNYDTRIS 300

Query: 319 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLFPV 360
           VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLV QLFPV
Sbjct: 301 VGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVHQLFPV 342

BLAST of IVF0001357 vs. TAIR 10
Match: AT2G38680.1 (5'-nucleotidases;magnesium ion binding )

HSP 1 Score: 402.9 bits (1034), Expect = 2.7e-112
Identity = 200/292 (68.49%), Postives = 242/292 (82.88%), Query Frame = 0

Query: 69  AEAVVADSDLLKKKLDALRFSGPQKLQVIADFDATLTRYWIDGCRG-QTSHGLLKQGNPE 128
           A  V+A    L  K+  +R +GP K QVIADFDATLTRY ++G RG QTSHGLL+QG+  
Sbjct: 8   ANTVIAHPRALTDKMTLIRDAGPSKFQVIADFDATLTRYRVNGLRGRQTSHGLLQQGDAY 67

Query: 129 YDTKRDELYKYYHPLEYSPTISIEEKTKLMEEWWGKSHSLLIEGGLTFDAIKQSVAAATI 188
           YD KR  LY +YHPLE SP I I+EKTKLMEEWWGK+H LLIEGGLT+DAIK+SVA ++I
Sbjct: 68  YDAKRQALYDHYHPLEISPVIPIDEKTKLMEEWWGKTHELLIEGGLTYDAIKKSVANSSI 127

Query: 189 AFREGVVELFELLEEKGVPVLIFSAGLADIIEEVLRQKLHRSFKNIRIVSNRMVFDDNGC 248
           AFREGV ELFE LE+K +PVLIFSAGLAD+IEEVLRQ L R+FKN++IVSNRMVF+D+G 
Sbjct: 128 AFREGVSELFEFLEKKEIPVLIFSAGLADVIEEVLRQNLDRTFKNVKIVSNRMVFNDDGQ 187

Query: 249 LVSFKGKTIHSLNKNEHALDMAAPLHDQLG-DVDGAINDSASVRKRTNVLLLGDHIGDLG 308
           LVSFKGK IH LNKNEHALDMAAPLHD+LG D+     ++ ++++R NVLL+GDH+GDL 
Sbjct: 188 LVSFKGKLIHVLNKNEHALDMAAPLHDRLGVDIGEEDEENVNMKERRNVLLMGDHLGDLR 247

Query: 309 MSDGLNYDTRISVGFLNDNIENSLDSYRNAFDIVYLNDASMLGVVKLVRQLF 359
           MSDGL+Y+TRIS+GFLNDNIE SL+SYR +FD+VYLNDA M G ++LV +LF
Sbjct: 248 MSDGLDYETRISIGFLNDNIEKSLESYRESFDLVYLNDAPMWGALELVSRLF 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7ZWS22.1e-4537.637-methylguanosine phosphate-specific 5'-nucleotidase A OS=Xenopus laevis OX=8355... [more]
Q2TAG64.7e-4537.637-methylguanosine phosphate-specific 5'-nucleotidase B OS=Xenopus laevis OX=8355... [more]
Q5ZKF61.4e-4435.147-methylguanosine phosphate-specific 5'-nucleotidase OS=Gallus gallus OX=9031 GN... [more]
Q7SYN41.8e-4435.11Cytosolic 5'-nucleotidase 3 OS=Danio rerio OX=7955 GN=nt5c3 PE=2 SV=2[more]
Q3UFY73.1e-4435.147-methylguanosine phosphate-specific 5'-nucleotidase OS=Mus musculus OX=10090 GN... [more]
Match NameE-valueIdentityDescription
A0A1S3BGK86.8e-204100.005'-nucleotidase OS=Cucumis melo OX=3656 GN=LOC103489365 PE=3 SV=1[more]
A0A5A7SXP56.8e-204100.005'-nucleotidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G0018... [more]
A0A0A0KRD51.6e-18496.515'-nucleotidase OS=Cucumis sativus OX=3659 GN=Csa_5G613460 PE=3 SV=1[more]
A0A6J1J0148.3e-17089.475'-nucleotidase OS=Cucurbita maxima OX=3661 GN=LOC111481459 PE=3 SV=1[more]
A0A6J1D8T41.4e-16988.825'-nucleotidase OS=Momordica charantia OX=3673 GN=LOC111018671 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008446729.11.06e-260100.00PREDICTED: 7-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis melo]... [more]
XP_004135048.17.86e-23696.517-methylguanosine phosphate-specific 5'-nucleotidase A [Cucumis sativus] >XP_011... [more]
XP_038893141.11.26e-22190.647-methylguanosine phosphate-specific 5'-nucleotidase A [Benincasa hispida] >XP_0... [more]
KAG6601237.19.41e-21789.47Cytosolic 5'-nucleotidase 3, partial [Cucurbita argyrosperma subsp. sororia] >KA... [more]
XP_022982686.11.90e-21689.477-methylguanosine phosphate-specific 5'-nucleotidase A [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G38680.12.7e-11268.495'-nucleotidases;magnesium ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.150.340coord: 106..187
e-value: 1.5E-94
score: 318.1
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 69..358
e-value: 0.0
score: 342.5
NoneNo IPR availablePANTHERPTHR130455'-NUCLEOTIDASEcoord: 70..357
NoneNo IPR availablePANTHERPTHR13045:SF0CYTOSOLIC 5'-NUCLEOTIDASE 3Acoord: 70..357
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 89..350
e-value: 1.5E-94
score: 318.1
IPR006434Pyrimidine 5'-nucleotidase, eukaryoticPFAMPF05822UMPH-1coord: 102..357
e-value: 1.2E-94
score: 316.5
IPR006434Pyrimidine 5'-nucleotidase, eukaryoticSFLDSFLDG01128C1.4:_5'-Nucleotidase_Likecoord: 69..358
e-value: 0.0
score: 342.5
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 62..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0001357.1IVF0001357.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0009117 nucleotide metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008253 5'-nucleotidase activity
molecular_function GO:0000287 magnesium ion binding