IVF0000904 (gene) Melon (IVF77) v1

Overview
NameIVF0000904
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionabscisic acid 8'-hydroxylase 4
Locationchr01: 350636 .. 353295 (-)
RNA-Seq ExpressionIVF0000904
SyntenyIVF0000904
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATATTATTCCTTTTTCTTTTCACTTTCTTTTCCATTTCATTTCTTAAAAGATCAAGAAATGAAAACTCTAAATCCCAAAATTCTCTTAAGCTTCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTCTATTCTCAAGATCCCAATATCTTTTTCTCACAGAAACAGAGAAGGTTCCTTTAGAACAAAAATTCAAACTTTTTATAAAAAACTTTATCCCAAAAAGAGAAAAAAAAAAGAAAAAAAGAAGGAAATTTCTAATTGGGTTTCTGTTTTTGGTGACAGATATGGTGAAATTTTTAAGACCAATATTTTGGGATGTCCTTGTGTCATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTGACAAACGCCCATTTATTCCGACCGACTTACCCGAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAATTACCATTCTAACCTCAGGAAGCTTGTTCTTAACTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTCTATAGAAGCCGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAACACCTTCCGTGAAATGAAAAAGGTCCGTTCTCTATTTTTCAACACACAACAAAATGGAATATAAAAAGATATTCTATAGAAACAGAGAGTTTCTTGGAATGATTTTGTTAATATAAAGTATATTCTCCACTGAAGTTATAAGTTTGAATTCTCACTCTCTCTGCACATTACATTGTTGAAAACTACACAATAGACAAAAGCTAAAGAAATATATTTTATGTGTGTATGTGTTTGGTATGAACAGTATTCATTCGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAAATGAGTACAAAGAGAAGCTAAAACAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCCACCAGATTACCAGGAACAGGATATTCAAAAGCATTATCAGTAAGATTTTAAACCATCTCCTATAATTAATCTTCTCCCAAATTTTCCCCATATTGTTTTTCGATTTTATTAATTTTCTTCTTTTTCTTAGGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTCGTTGGTCATCTTTTGAACTATAGAGATGAAAATGGTGAAAGTTTAAGTGAAGATCAAATCGCTGACAATATCATCGGAGTTCTATTCGCCGCCCAAGACACCACCGCCACTGTCCTCACTTGGATCCTTAAATATCTTCACGACAACTTCAAACTTTTCGAGGCTGTTAAGGTATATAACCATTTTTCTCCTTTTTCAATATTCCAATAATTTCAAACTTGTTACCATTAAATCCTTGGTGGAAAATATATTATAGCATGGAACATAAAATTTGGACAAAACCACGTGCATGCTCATTTTAATGATGTTTTTGGTGAATTTTTCAGGCAGAGCAGATGGAAATTTATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGGGTCAGATTAAGGACATGCCCTTCACTCATAGGGTATGTTTCATTTTTGTCATCCATTTTTCTCCTCACACTTTAAAAGAAAACTAAAAGCATGTGATGATGATTATTTTTCAGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTGGTTGATGTAGAATATAAAGGTATATACCATGGTTCTTTTGGTCAATCTCACACTTTCTTTTCTTAATTAATGACAATATCAACATATAAGGGGTTGAATAAAGAAATAATTAATCATGTTTATAGGGTACCTTATCCCAAAGGGTTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCACGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTAAGCTTTGAATTATATTCATATTTTTATTCTTTTATGGTTGTAAGCAATAAGCTAAGTTTTGATAATACTGTTAATTGGTAATGTCTTAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTTCATCACCTCATTACCAAATTTAGGTAACTAACTTCAAGTAATTCAAACCCCAAAAAAGATTACAAATATATTTTCTTTTCCCTATTTGTTGTTGTTTTGTTATTAATAATAAAAAAGAAGAGGAATTAATTAATTAAGAAATTGTGTTACGTTTGTAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCGATGCCTCTCCAAGGCCTACCCTCCAGATTTTTCAAACAAACAACCACCAACCAATAGCCTTTCATTTCATTATTATTCATATAATAAAAATCCATATCATTTTATTTTTCTAAATCTTCCAACCAATTATTATATTATGATTCTAAAATTAATGCCCTAGCTTCTTCACCTAAACCCTCTTTCTTTTTACCCCCTTATCAATGCTTGTATTGATTTTTATTTCGAGTATTGAGAAAGTAAGGGATGTATTTTCATATTTGTTGTCTCTTATCATCGGGAG

mRNA sequence

ATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATATTATTCCTTTTTCTTTTCACTTTCTTTTCCATTTCATTTCTTAAAAGATCAAGAAATGAAAACTCTAAATCCCAAAATTCTCTTAAGCTTCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTCTATTCTCAAGATCCCAATATCTTTTTCTCACAGAAACAGAGAAGATATGGTGAAATTTTTAAGACCAATATTTTGGGATGTCCTTGTGTCATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTGACAAACGCCCATTTATTCCGACCGACTTACCCGAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAATTACCATTCTAACCTCAGGAAGCTTGTTCTTAACTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTCTATAGAAGCCGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAACACCTTCCGTGAAATGAAAAAGTATTCATTCGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAAATGAGTACAAAGAGAAGCTAAAACAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCCACCAGATTACCAGGAACAGGATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTCGTTGGTCATCTTTTGAACTATAGAGATGAAAATGGTGAAAGTTTAAGTGAAGATCAAATCGCTGACAATATCATCGGAGTTCTATTCGCCGCCCAAGACACCACCGCCACTGTCCTCACTTGGATCCTTAAATATCTTCACGACAACTTCAAACTTTTCGAGGCTGTTAAGGCAGAGCAGATGGAAATTTATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGGGTCAGATTAAGGACATGCCCTTCACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTGGTTGATGTAGAATATAAAGGGTACCTTATCCCAAAGGGTTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCACGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTTCATCACCTCATTACCAAATTTAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCGATGCCTCTCCAAGGCCTACCCTCCAGATTTTTCAAACAAACAACCACCAACCAATAGCCTTTCATTTCATTATTATTCATATAATAAAAATCCATATCATTTTATTTTTCTAAATCTTCCAACCAATTATTATATTATGATTCTAAAATTAATGCCCTAGCTTCTTCACCTAAACCCTCTTTCTTTTTACCCCCTTATCAATGCTTGTATTGATTTTTATTTCGAGTATTGAGAAAGTAAGGGATGTATTTTCATATTTGTTGTCTCTTATCATCGGGAG

Coding sequence (CDS)

ATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATATTATTCCTTTTTCTTTTCACTTTCTTTTCCATTTCATTTCTTAAAAGATCAAGAAATGAAAACTCTAAATCCCAAAATTCTCTTAAGCTTCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTCTATTCTCAAGATCCCAATATCTTTTTCTCACAGAAACAGAGAAGATATGGTGAAATTTTTAAGACCAATATTTTGGGATGTCCTTGTGTCATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTGACAAACGCCCATTTATTCCGACCGACTTACCCGAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAATTACCATTCTAACCTCAGGAAGCTTGTTCTTAACTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTCTATAGAAGCCGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAACACCTTCCGTGAAATGAAAAAGTATTCATTCGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAAATGAGTACAAAGAGAAGCTAAAACAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCCACCAGATTACCAGGAACAGGATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTCGTTGGTCATCTTTTGAACTATAGAGATGAAAATGGTGAAAGTTTAAGTGAAGATCAAATCGCTGACAATATCATCGGAGTTCTATTCGCCGCCCAAGACACCACCGCCACTGTCCTCACTTGGATCCTTAAATATCTTCACGACAACTTCAAACTTTTCGAGGCTGTTAAGGCAGAGCAGATGGAAATTTATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGGGTCAGATTAAGGACATGCCCTTCACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTGGTTGATGTAGAATATAAAGGGTACCTTATCCCAAAGGGTTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCACGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTTCATCACCTCATTACCAAATTTAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCGATGCCTCTCCAAGGCCTACCCTCCAGATTTTTCAAACAAACAACCACCAACCAATAG

Protein sequence

MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ
Homology
BLAST of IVF0000904 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 638.3 bits (1645), Expect = 7.2e-182
Identity = 305/469 (65.03%), Postives = 377/469 (80.38%), Query Frame = 0

Query: 4   LSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDP 63
           L VP+ I  L L       +  +   + +NS+     KLPPGSMG P++GETLQLYSQ+P
Sbjct: 7   LVVPILILCLLL-------VRVIVSKKKKNSRG----KLPPGSMGWPYLGETLQLYSQNP 66

Query: 64  NIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 123
           N+FF+ KQ+RYGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+A
Sbjct: 67  NVFFTSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSA 126

Query: 124 LFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYS 183
           LFFHQG+YHS++RKLV +S   E +R L+P IE  A+S+  SWA    +++T++EMKK++
Sbjct: 127 LFFHQGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFA 186

Query: 184 FEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEI 243
           F+VGI+A+FG LE+ YKE LK  Y I+DKGYN FP  LPGT Y KAL ARK+L+ I+ EI
Sbjct: 187 FDVGILAIFGHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEI 246

Query: 244 IMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYL 303
           I ERREKR  + DF+GHLLN+++E G  L+++QIADNIIGVLFAAQDTTA+ LTWILKYL
Sbjct: 247 ICERREKRALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYL 306

Query: 304 HDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREA 363
           HD+ KL EAVKAEQ  IY  N   K PL+W Q ++MP TH+V++ESLRMASIISFTFREA
Sbjct: 307 HDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREA 366

Query: 364 VVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHS 423
           VVDVEYKGYLIPKGWKVMPLFRNIHHN +YF +P +FDPSRFEV P+PNTFMPFG+GVH+
Sbjct: 367 VVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHA 426

Query: 424 CPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRF 473
           CPGNELAKL+ILI LHHL++ FRWEV+G + G++Y PFP+P  GLP+ F
Sbjct: 427 CPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of IVF0000904 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 1.8e-180
Identity = 313/472 (66.31%), Postives = 373/472 (79.03%), Query Frame = 0

Query: 8   LYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFF 67
           LYI+   +F+  + S  F     N +S  +N+ KLPPGSMG P+IGETLQLYSQDPN FF
Sbjct: 9   LYIS---MFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFF 68

Query: 68  SQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFH 127
             +QRR+GEIFKT ILGCPCVMLAS  AARFVLV  A+LF+PTYPKSKE +IG +A+FFH
Sbjct: 69  INRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFH 128

Query: 128 QGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVG 187
           QG+YH++LRKLV   L+ E +R  +P IE  +ISA +SW   GHV+NT+ EMKK+SFEVG
Sbjct: 129 QGDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSW-VGGHVVNTYHEMKKFSFEVG 188

Query: 188 IIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMER 247
           I+A+FG L+   KE+LK+ Y I+DKGYN FP  LPGT Y KAL ARKKL +IL EII E 
Sbjct: 189 ILAIFGHLDGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREM 248

Query: 248 REKRVTERDFVGHLLNYRDENG-ESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDN 307
           +EK+  E+  +   LN ++E G   L+EDQIADNIIGVLFAAQDTTA+VLTWI+KYLHDN
Sbjct: 249 KEKKTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDN 308

Query: 308 FKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVD 367
            KL E VKAEQ  I++ N +    L+W Q + MP T RVVLE+LRMASIISF FREAV D
Sbjct: 309 PKLLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVAD 368

Query: 368 VEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPG 427
           VEYKGYLIPKGWKVMPLFRNIHHN E+FPDP  FDPSRFE AP+PNTFMPFG+GVH+CPG
Sbjct: 369 VEYKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPG 428

Query: 428 NELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTT 479
           NELAKLEILI+ HHL+TKFRWEV GS SG++YGPFP+PL GL +RF+K T+T
Sbjct: 429 NELAKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFWKTTST 476

BLAST of IVF0000904 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.1e-169
Identity = 282/446 (63.23%), Postives = 352/446 (78.92%), Query Frame = 0

Query: 35  KSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAA 94
           + +  L+LPPGSMG P++GETLQLYSQDPN+FF+ KQ+RYGEIFKTN+LGCPCVMLAS  
Sbjct: 44  QKKKKLRLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPE 103

Query: 95  AARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPS 154
           AARFVLV+ A LF+PTYP SKE MIGP+ALFFHQG YH  LR+LV  +L+ + LR LVP 
Sbjct: 104 AARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPD 163

Query: 155 IEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVF-GKLENEYKEKLKQKYCILDKG 214
           ++AA  +   +W + GHV +TF  MKK SF+VG++ +F G+L   ++E+L+  Y ++++G
Sbjct: 164 VDAAVAATLAAW-SGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERG 223

Query: 215 YNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGES-- 274
           YNCFP R PGT Y KA+ ARK+LR IL EI+ ERR +     D +G L+  RD+      
Sbjct: 224 YNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDGTAGAV 283

Query: 275 --LSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKM 334
             L++DQIADN++GVLFAAQDTTA+VLTWILKYLHD+ KL EAVKAEQM IY  N  GK 
Sbjct: 284 ALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKR 343

Query: 335 PLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHH 394
           PL+W Q + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPLFRNIHH
Sbjct: 344 PLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHH 403

Query: 395 NHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEV 454
           N +YF DP  FDPSRF+VAPRP+TF+PFG+GVH+CPGNELAKLE+L+L+H L+T +RWE+
Sbjct: 404 NPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWEI 463

Query: 455 EGSQSGVEYGPFPMPLQGLPSRFFKQ 476
            G+   VEY PFP+P  GL ++ +KQ
Sbjct: 464 VGASDEVEYSPFPVPRGGLNAKLWKQ 488

BLAST of IVF0000904 vs. ExPASy Swiss-Prot
Match: Q6ZDE3 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.2e-167
Identity = 282/450 (62.67%), Postives = 352/450 (78.22%), Query Frame = 0

Query: 35  KSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAA 94
           + +  L+LPPGSMG P++GETLQLYSQDPN+FF+ KQ+RYGEIFKTN+LGCPCVMLAS  
Sbjct: 44  QKKKKLRLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPE 103

Query: 95  AARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPS 154
           AARFVLV+ A LF+PTYP SKE MIGP+ALFFHQG YH  LR+LV  +L+ + LR LVP 
Sbjct: 104 AARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPD 163

Query: 155 IEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVF-GKLENEYKEKLKQKYCILDKG 214
           ++AA  +   +W + GHV +TF  MKK SF+VG++ +F G+L   ++E+L+  Y ++++G
Sbjct: 164 VDAAVAATLAAW-SGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERG 223

Query: 215 YNCFPTRLPGTGYSKALSARKKLREILGEIIMERREK----RVTERDFVGHLLNYRDENG 274
           YNCFP R PGT Y KA+ ARK+LR IL EI+ ERR +         D +G L+  RD+  
Sbjct: 224 YNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGGGGGDDLLGGLMRSRDDGT 283

Query: 275 ES----LSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNG 334
                 L++DQIADN++GVLFAAQDTTA+VLTWILKYLHD+ KL EAVKAEQM IY  N 
Sbjct: 284 AGAVALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANE 343

Query: 335 EGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFR 394
            GK PL+W Q + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPLFR
Sbjct: 344 GGKRPLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFR 403

Query: 395 NIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKF 454
           NIHHN +YF DP  FDPSRF+VAPRP+TF+PFG+GVH+CPGNELAKLE+L+L+H L+T +
Sbjct: 404 NIHHNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAY 463

Query: 455 RWEVEGSQSGVEYGPFPMPLQGLPSRFFKQ 476
           RWE+ G+   VEY PFP+P  GL ++ +KQ
Sbjct: 464 RWEIVGASDEVEYSPFPVPRGGLNAKLWKQ 492

BLAST of IVF0000904 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.1e-166
Identity = 289/482 (59.96%), Postives = 363/482 (75.31%), Query Frame = 0

Query: 3   ALSVPLYITILFLFLFTFFS-ISFLKRSRNE--NSKSQNSLKLPPGSMGLPWIGETLQLY 62
           A S  + I I F     F   + +  R R +      + +++LPPGSMG P+IGETLQLY
Sbjct: 2   AASFVIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLY 61

Query: 63  SQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMI 122
           SQDPN+FF+ KQ+RYGEIFKT+ILGCPCVMLAS  AARFVLVT AHLF+PTYP+SKE MI
Sbjct: 62  SQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMI 121

Query: 123 GPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREM 182
           GP+ALFFHQG+YH  LRKLV   L  + LR LVP +EAA  S   SW   G+V +TF  M
Sbjct: 122 GPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW--DGNVSSTFHAM 181

Query: 183 KKYSFEVGIIAVF-GKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLRE 242
           K+ SF+VGI+ +F G+L+   K +L+Q Y I++KGYN FP   PGT Y KA+ AR++L  
Sbjct: 182 KRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHG 241

Query: 243 ILGEIIMERREKRVTERDFVGHLLNYR-DENGESLSEDQIADNIIGVLFAAQDTTATVLT 302
           +L +I+ ERR +     D +G L+  R  ++G  L+++Q+ADNIIGVLFAAQDTTA+VLT
Sbjct: 242 VLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLT 301

Query: 303 WILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIIS 362
           WI+KYLHD+ KL EAV+AEQ  I   N  G++PL+W Q + M  TH+V+LESLRMASIIS
Sbjct: 302 WIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIIS 361

Query: 363 FTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPF 422
           FTFREAV DVEYKG+LIPKGWKVMPLFRNIHHN +YF DP  FDPSRF+V+PRPNTFMPF
Sbjct: 362 FTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPF 421

Query: 423 GNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTT 480
           GNGVH+CPGNELAKLE+L+L+HHL+T +RWE+ GS   VEY PFP+P  GL ++ ++  +
Sbjct: 422 GNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWRDDS 481

BLAST of IVF0000904 vs. ExPASy TrEMBL
Match: A0A1S3BZQ3 (abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.2e-280
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180

Query: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

BLAST of IVF0000904 vs. ExPASy TrEMBL
Match: A0A5A7SMJ8 (Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00490 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.0e-255
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 120

Query: 167 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 226
           AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY
Sbjct: 121 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 466
           VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ
Sbjct: 361 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 420

Query: 467 GLPSRFFKQTTTNQ 481
           GLPSRFFKQTTTNQ
Sbjct: 421 GLPSRFFKQTTTNQ 434

BLAST of IVF0000904 vs. ExPASy TrEMBL
Match: A0A0A0K6K8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.2e-248
Identity = 422/434 (97.24%), Postives = 427/434 (98.39%), Query Frame = 0

Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW 120

Query: 167 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 226
           AAAGHVINTF EMKKYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT Y
Sbjct: 121 AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDENGE+LSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGENLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSW QIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWSQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 466
           VAPR NTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+Q
Sbjct: 361 VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ 420

Query: 467 GLPSRFFKQTTTNQ 481
           GLP+RFFKQTTTNQ
Sbjct: 421 GLPARFFKQTTTNQ 434

BLAST of IVF0000904 vs. ExPASy TrEMBL
Match: A0A6J1I8M8 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.7e-242
Identity = 419/481 (87.11%), Postives = 443/481 (92.10%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYI I+ LFL  FFSISFLK  RNE  KSQ +LKLPPGSMGLPW GETLQLYS
Sbjct: 1   MEALSVPLYI-IISLFLLAFFSISFLK--RNEQPKSQEALKLPPGSMGLPWFGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRR+GEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKET+IG
Sbjct: 61  QDPNIFFSEKQRRFGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETLIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLR-TLVPSIEAAAISATDSWAAAGHVINTFREM 180
           PAALFFHQGNYHSNLRKLV NSLSL+RLR TLVP IEAAAISA DSWAA  HV+NTF +M
Sbjct: 121 PAALFFHQGNYHSNLRKLVQNSLSLQRLRTTLVPHIEAAAISAADSWAATAHVVNTFLQM 180

Query: 181 KKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREI 240
           KK+SFEV I+AVFGKLE  Y EKLK+ YCILDKGYN FPTRLPGT  SKALSARKKLREI
Sbjct: 181 KKFSFEVAIVAVFGKLETHYSEKLKENYCILDKGYNSFPTRLPGTACSKALSARKKLREI 240

Query: 241 LGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWI 300
           L EII+ERREK   ERDFVG LL+YRDENGE LSE+QIADN+IGVLFAAQDTTA+VLTWI
Sbjct: 241 LREIIVERREKEEAERDFVGQLLSYRDENGERLSEEQIADNVIGVLFAAQDTTASVLTWI 300

Query: 301 LKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFT 360
           LKYLHD+FKLFEAV+AEQMEIYRRNGEGKMPLSW QIKDMPFTHRVVLESLRMASIISFT
Sbjct: 301 LKYLHDSFKLFEAVRAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFT 360

Query: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGN 420
           FREAVVDVEYKGY IPKGWKVMPLFRNIHHNH+YF DPH FDPSRFEVAPRPNTFMPFGN
Sbjct: 361 FREAVVDVEYKGYRIPKGWKVMPLFRNIHHNHDYFNDPHTFDPSRFEVAPRPNTFMPFGN 420

Query: 421 GVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTN 480
           GVHSCPGNELAKLEILILLHHL+TKFRWEV G QSGV+YGPFP+P+ GLP+RFFKQTTTN
Sbjct: 421 GVHSCPGNELAKLEILILLHHLVTKFRWEVVGPQSGVQYGPFPVPVHGLPARFFKQTTTN 478

BLAST of IVF0000904 vs. ExPASy TrEMBL
Match: A0A6J1EHU3 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111434488 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 3.0e-239
Identity = 411/480 (85.62%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           ME LS  LY+ I F+      S   L   RNE  K + SL+LPPGSMGLPWIGETLQLYS
Sbjct: 1   MEILSFTLYLIIFFI-----SSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180
           PAALFFHQGNYHSN+RKLV NSL L  LR LVP IE AAISA +SWAAAGHVINTF EMK
Sbjct: 121 PAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMK 180

Query: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240
           K+SF+VGI+AVFGKLE++Y EKLK+ YCILDKGYN FPTRLPGT YSKALSARKKLREIL
Sbjct: 181 KFSFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERD VGHLL++RDENG  LSEDQIADNIIGVLFAAQDTTA+VLTWIL
Sbjct: 241 GEIIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDN KLFEAV+AEQMEIYRRNG+GKMPLSW QIKDMP THRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNLKLFEAVRAEQMEIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420
           REAVVDVEYKG+LIPKGWKVMPLFRNIHHN +YFP+P IFDPSRFEVAPRPNTFMPFGNG
Sbjct: 361 REAVVDVEYKGFLIPKGWKVMPLFRNIHHNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480
           VHSCPGNELAKLEIL+LLHHLITKFRWEV GSQSGV+YGPFPMP+ GLP+RFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

BLAST of IVF0000904 vs. NCBI nr
Match: XP_008454947.1 (PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180

Query: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

BLAST of IVF0000904 vs. NCBI nr
Match: XP_004137112.1 (abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical protein Csa_017084 [Cucumis sativus])

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 463/480 (96.46%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITIL L LF FFSISFLKR+RNEN KSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSWAAAGHVINTF EMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180

Query: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT YSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERD VGHLLN+RDENGE+LSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDLVGHLLNFRDENGENLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSW QIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPR NTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+QGLP+RFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480

BLAST of IVF0000904 vs. NCBI nr
Match: KAA0031296.1 (abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 885 bits (2288), Expect = 0.0
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 120

Query: 167 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 226
           AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY
Sbjct: 121 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 466
           VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ
Sbjct: 361 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 420

Query: 467 GLPSRFFKQTTTNQ 480
           GLPSRFFKQTTTNQ
Sbjct: 421 GLPSRFFKQTTTNQ 434

BLAST of IVF0000904 vs. NCBI nr
Match: XP_038887333.1 (abscisic acid 8'-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 881 bits (2276), Expect = 0.0
Identity = 442/484 (91.32%), Postives = 456/484 (94.21%), Query Frame = 0

Query: 1   MEALSVPLYITIL---FLFLFTFF-SISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETL 60
           MEALSV LYI I+   FLFLF FF SISFLKR  N+  KSQ  LKLPPGSMGLPWIGETL
Sbjct: 1   MEALSVTLYIIIIIIIFLFLFAFFFSISFLKR--NQKPKSQKVLKLPPGSMGLPWIGETL 60

Query: 61  QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE 120
           QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE
Sbjct: 61  QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE 120

Query: 121 TMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTF 180
           TMIGPAALFFHQGNYHSNLRKLV NSLSLERLR LVP IEAAAISATDSWAAAGHVINTF
Sbjct: 121 TMIGPAALFFHQGNYHSNLRKLVQNSLSLERLRILVPHIEAAAISATDSWAAAGHVINTF 180

Query: 181 REMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKL 240
            EMKKYSFEVGI+AVF KLENEYKEKLK+KY ILDKGYNCFPTRLPGT YSKALSARKKL
Sbjct: 181 LEMKKYSFEVGIVAVFEKLENEYKEKLKEKYSILDKGYNCFPTRLPGTAYSKALSARKKL 240

Query: 241 REILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVL 300
           REILGEIIMERREKRVTERD VGHLLN+RDENGE+LSEDQIADNIIGVLFAAQDTTAT L
Sbjct: 241 REILGEIIMERREKRVTERDLVGHLLNFRDENGETLSEDQIADNIIGVLFAAQDTTATAL 300

Query: 301 TWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASII 360
           TWILK+LHDN KLFEAVKAEQMEIYRRNG+GKMPLSW QIKDMPFTHRVVLESLRMASII
Sbjct: 301 TWILKFLHDNLKLFEAVKAEQMEIYRRNGDGKMPLSWAQIKDMPFTHRVVLESLRMASII 360

Query: 361 SFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMP 420
           SFTFREAVVD+EYKGYLIPKGWKVMPLFRNIHHN EYFPDPHIFDPSRFE+AP+PNTFMP
Sbjct: 361 SFTFREAVVDMEYKGYLIPKGWKVMPLFRNIHHNPEYFPDPHIFDPSRFELAPKPNTFMP 420

Query: 421 FGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQT 480
           FGNGVHSCPGNELAKLEILILLHHLITKFRWEV GS+SGVEYGPFPMP+ GLP+RFFK T
Sbjct: 421 FGNGVHSCPGNELAKLEILILLHHLITKFRWEVVGSESGVEYGPFPMPVHGLPARFFKHT 480

BLAST of IVF0000904 vs. NCBI nr
Match: XP_023553809.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845 bits (2184), Expect = 2.01e-307
Identity = 419/481 (87.11%), Postives = 444/481 (92.31%), Query Frame = 0

Query: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYI I+ LFL  FFSISFLKR  NE  K+Q +LKLPPGSMGLPW GETLQLYS
Sbjct: 1   MEALSVPLYI-IISLFLLAFFSISFLKR--NEQPKAQEALKLPPGSMGLPWFGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRR+GEIFKTNILGCPCVMLASAAAARFV VTNAHLFRPTYPKSKET+IG
Sbjct: 61  QDPNIFFSEKQRRFGEIFKTNILGCPCVMLASAAAARFVFVTNAHLFRPTYPKSKETLIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRT-LVPSIEAAAISATDSWAAAGHVINTFREM 180
           PAALFFHQGNYHSNLRKLV NSL LERLRT LVP IEAAAISA DSWAA GHV+NTF +M
Sbjct: 121 PAALFFHQGNYHSNLRKLVQNSLCLERLRTTLVPHIEAAAISAADSWAATGHVVNTFLQM 180

Query: 181 KKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREI 240
           KK+SFEV I+AVFGKLE +Y EKLK+ YCILDKGYN FPTRLPGT YSKALSARKKLREI
Sbjct: 181 KKFSFEVAIVAVFGKLETQYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREI 240

Query: 241 LGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWI 300
           L EII+ERREK   ERDFVG LL+YRDENGE LSE+QIADN+IGVLFAAQDTTA+VLTWI
Sbjct: 241 LREIIVERREKGAAERDFVGQLLSYRDENGEGLSEEQIADNVIGVLFAAQDTTASVLTWI 300

Query: 301 LKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFT 360
           LKYLHD+ KLFEAV+AEQMEIYRRNGEGKMPLSW QIKDMPFTHRVVLESLRMASIISFT
Sbjct: 301 LKYLHDSLKLFEAVRAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFT 360

Query: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGN 420
           FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNH+YF DPH FDPSRFEVAPRPNTFMPFGN
Sbjct: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHDYFNDPHTFDPSRFEVAPRPNTFMPFGN 420

Query: 421 GVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTN 480
           GVHSCPGNELAKLEILILLHHL+TKFRWEV G QSGV+YGPFP+P+ GLP+RFFKQTTTN
Sbjct: 421 GVHSCPGNELAKLEILILLHHLVTKFRWEVVGPQSGVQYGPFPVPVHGLPARFFKQTTTN 478

BLAST of IVF0000904 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 638.3 bits (1645), Expect = 5.1e-183
Identity = 305/469 (65.03%), Postives = 377/469 (80.38%), Query Frame = 0

Query: 4   LSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDP 63
           L VP+ I  L L       +  +   + +NS+     KLPPGSMG P++GETLQLYSQ+P
Sbjct: 7   LVVPILILCLLL-------VRVIVSKKKKNSRG----KLPPGSMGWPYLGETLQLYSQNP 66

Query: 64  NIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 123
           N+FF+ KQ+RYGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+A
Sbjct: 67  NVFFTSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSA 126

Query: 124 LFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYS 183
           LFFHQG+YHS++RKLV +S   E +R L+P IE  A+S+  SWA    +++T++EMKK++
Sbjct: 127 LFFHQGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFA 186

Query: 184 FEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEI 243
           F+VGI+A+FG LE+ YKE LK  Y I+DKGYN FP  LPGT Y KAL ARK+L+ I+ EI
Sbjct: 187 FDVGILAIFGHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEI 246

Query: 244 IMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYL 303
           I ERREKR  + DF+GHLLN+++E G  L+++QIADNIIGVLFAAQDTTA+ LTWILKYL
Sbjct: 247 ICERREKRALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYL 306

Query: 304 HDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREA 363
           HD+ KL EAVKAEQ  IY  N   K PL+W Q ++MP TH+V++ESLRMASIISFTFREA
Sbjct: 307 HDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREA 366

Query: 364 VVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHS 423
           VVDVEYKGYLIPKGWKVMPLFRNIHHN +YF +P +FDPSRFEV P+PNTFMPFG+GVH+
Sbjct: 367 VVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHA 426

Query: 424 CPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRF 473
           CPGNELAKL+ILI LHHL++ FRWEV+G + G++Y PFP+P  GLP+ F
Sbjct: 427 CPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of IVF0000904 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 260/462 (56.28%), Postives = 345/462 (74.68%), Query Frame = 0

Query: 8   LYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFF 67
           L++T+    LF +F    + + R  +SK    L LPPG+MG P++GET QLYSQDPN+FF
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSK----LPLPPGTMGWPYVGETFQLYSQDPNVFF 65

Query: 68  SQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFH 127
             KQ+RYG +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A+FFH
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 128 QGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVG 187
           QG+YH+ LRKLVL +   E +R +VP IE+ A  +  SW   G +INT++EMK Y+F V 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW--EGTMINTYQEMKTYTFNVA 185

Query: 188 IIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMER 247
           ++++FGK E  Y+E LK+ Y IL+KGYN  P  LPGT + K++ ARK+L +IL  I+ ER
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 248 REKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNF 307
           R+   +  D +G  +  ++E    L+++QIADNIIGV+FAA+DTTA+V++WILKYL +N 
Sbjct: 246 RQNGSSHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENP 305

Query: 308 KLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDV 367
            + EAV  EQM I +   EG+  L+WG  K MP T RV+ E+LR+ASI+SFTFREAV DV
Sbjct: 306 NVLEAVTEEQMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV 365

Query: 368 EYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGN 427
           EY+GYLIPKGWKV+PLFRNIHH+ + F +P  FDPSRFEVAP+PNTFMPFGNG HSCPGN
Sbjct: 366 EYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGN 425

Query: 428 ELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLP 470
           ELAKLE+ I++HHL TK+ W + G+  G++YGPF +P  GLP
Sbjct: 426 ELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLP 456

BLAST of IVF0000904 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 540.0 bits (1390), Expect = 1.9e-153
Identity = 267/466 (57.30%), Postives = 344/466 (73.82%), Query Frame = 0

Query: 4   LSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYSQDP 63
           L + L    LFL L  F  I+ ++RS      S   L LPPG+MG P++GET QLYSQDP
Sbjct: 6   LFLTLSAAALFLCLLRF--IAGVRRS------SSTKLPLPPGTMGYPYVGETFQLYSQDP 65

Query: 64  NIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 123
           N+FF+ KQRRYG +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A
Sbjct: 66  NVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQA 125

Query: 124 LFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMKKYS 183
           +FFHQG+YHS LRKLVL +   + +R +VP IE+ A  + +SW   G  +NT++EMK Y+
Sbjct: 126 IFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYT 185

Query: 184 FEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREILGEI 243
           F V +I++ GK E  Y+E LK+ Y IL+KGYN  P  LPGT + KA+ ARK+L +IL  I
Sbjct: 186 FNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANI 245

Query: 244 IMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYL 303
           + +RR+   +  D +G  +    E+   L+++QIADNIIGV+FAA+DTTA+VLTWILKYL
Sbjct: 246 LSKRRQNPSSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYL 305

Query: 304 HDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTFREA 363
            DN  + EAV  EQM I +   EG+  L+W   K MP T+RV+ E+LR A+I+SFTFREA
Sbjct: 306 ADNPTVLEAVTEEQMAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREA 365

Query: 364 VVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHS 423
           V DVEY+GYLIPKGWKV+PLFRNIHHN + F DP  FDPSRFEVAP+PNTFMPFG+G+HS
Sbjct: 366 VEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHS 425

Query: 424 CPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLP 470
           CPGNELAKLEI +L+HHL TK+RW + G   G++YGPF +P  GLP
Sbjct: 426 CPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLP 456

BLAST of IVF0000904 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 516.2 bits (1328), Expect = 2.9e-146
Identity = 252/436 (57.80%), Postives = 319/436 (73.17%), Query Frame = 0

Query: 37  QNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAA 96
           +  L+LPPGSMGLP+IGETL+LY+++PN FF+ +Q +YG+IFKT+ILGCPCVM++S  AA
Sbjct: 44  EQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAA 103

Query: 97  RFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIE 156
           R VLV+ AHLF+PTYP SKE MIGP ALFFHQG YHS L++LV +S     LR  V  IE
Sbjct: 104 RMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIE 163

Query: 157 AAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFG-KLENEYKEKLKQKYCILDKGYN 216
              +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y  L++GYN
Sbjct: 164 LLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYN 223

Query: 217 CFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSED 276
             P  LPGT + K++ AR +L E L ++I +RRE    E   +G LL  +D+    LS+ 
Sbjct: 224 SMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDS 283

Query: 277 QIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQ 336
           QIADNIIGV+FAA DTTA+VLTW+LKYLHD+  L + V  EQ  I ++  +    +SW  
Sbjct: 284 QIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWED 343

Query: 337 IKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFP 396
            + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR IHH+ E+FP
Sbjct: 344 TRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFP 403

Query: 397 DPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSG 456
           DP  FDPSRFEVAP+P T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRWEV G + G
Sbjct: 404 DPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEG 463

Query: 457 VEYGPFPMPLQGLPSR 472
           ++YGPFP+P +GLP R
Sbjct: 464 IQYGPFPVPKKGLPIR 478

BLAST of IVF0000904 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 516.2 bits (1328), Expect = 2.9e-146
Identity = 252/436 (57.80%), Postives = 319/436 (73.17%), Query Frame = 0

Query: 37  QNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAA 96
           +  L+LPPGSMGLP+IGETL+LY+++PN FF+ +Q +YG+IFKT+ILGCPCVM++S  AA
Sbjct: 44  EQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAA 103

Query: 97  RFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIE 156
           R VLV+ AHLF+PTYP SKE MIGP ALFFHQG YHS L++LV +S     LR  V  IE
Sbjct: 104 RMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIE 163

Query: 157 AAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFG-KLENEYKEKLKQKYCILDKGYN 216
              +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y  L++GYN
Sbjct: 164 LLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYN 223

Query: 217 CFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSED 276
             P  LPGT + K++ AR +L E L ++I +RRE    E   +G LL  +D+    LS+ 
Sbjct: 224 SMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDS 283

Query: 277 QIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQ 336
           QIADNIIGV+FAA DTTA+VLTW+LKYLHD+  L + V  EQ  I ++  +    +SW  
Sbjct: 284 QIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWED 343

Query: 337 IKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFP 396
            + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR IHH+ E+FP
Sbjct: 344 TRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFP 403

Query: 397 DPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSG 456
           DP  FDPSRFEVAP+P T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRWEV G + G
Sbjct: 404 DPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEG 463

Query: 457 VEYGPFPMPLQGLPSR 472
           ++YGPFP+P +GLP R
Sbjct: 464 IQYGPFPVPKKGLPIR 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJK27.2e-18265.03Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
A9QNE71.8e-18066.31Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q09J784.1e-16963.23Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q6ZDE32.2e-16762.67Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
Q0J1851.1e-16659.96Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
Match NameE-valueIdentityDescription
A0A1S3BZQ31.2e-280100.00abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1[more]
A0A5A7SMJ81.0e-255100.00Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0K6K81.2e-24897.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1[more]
A0A6J1I8M81.7e-24287.11abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 ... [more]
A0A6J1EHU33.0e-23985.63abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC11143448... [more]
Match NameE-valueIdentityDescription
XP_008454947.10.0100.00PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo][more]
XP_004137112.10.096.46abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical prot... [more]
KAA0031296.10.0100.00abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic a... [more]
XP_038887333.10.091.32abscisic acid 8'-hydroxylase 4 [Benincasa hispida][more]
XP_023553809.12.01e-30787.11abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G19270.15.1e-18365.03cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.11.4e-15356.28cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.11.9e-15357.30cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT2G29090.12.9e-14657.80cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.22.9e-14657.80cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 343..361
score: 22.97
coord: 383..407
score: 27.21
coord: 424..447
score: 30.32
coord: 71..90
score: 27.21
coord: 414..424
score: 47.18
coord: 276..293
score: 21.19
IPR001128Cytochrome P450PRINTSPR00385P450coord: 344..355
score: 33.62
coord: 415..424
score: 47.79
coord: 424..435
score: 39.37
coord: 287..304
score: 36.48
IPR001128Cytochrome P450PFAMPF00067p450coord: 43..453
e-value: 3.1E-71
score: 240.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 31..474
e-value: 6.4E-118
score: 396.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..472
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 14..474
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 14..474
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 417..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000904.1IVF0000904.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen