IVF0000871 (gene) Melon (IVF77) v1

Overview
NameIVF0000871
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionTyrosine--tRNA ligase
Locationchr09: 22039559 .. 22042838 (+)
RNA-Seq ExpressionIVF0000871
SyntenyIVF0000871
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACAGAAGCACCTTCGCAAGCTCCATCCGAGGATTTACAGTCCCTCTCAATCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCAAACTCTTCAAACCAGTATGAAGTTTTTGTGTTTGACACTTGTTGTTTTCGTATCCATTTCTCTCTTGATTTGTATCTGAATCGTAATTTACATGACTTGAACTGTATTTTTCTATAGATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGTAAAGTTTACAGGAATCTTTGAGTCCGGTCTTTTAAGTTGTAGTTGATAGCTATAAACTAAGTGAATTGGGTTTTGGTTTTCTGTGGCAATCTACCCTGTTTACATCGTGAATTTCAGCAGAGAAAAAAAATGGGGGGGACCAAGCAATTTTTGGGTTAATCTAAACGTAATTGAAACTGAACAATTTCCAAGTGAGTTTCCCCCACCCGTATCCATTGAATGTGAATTGCTGATTTATGTGCCTTTGTTTCCTCACACAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGATGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGCTATTTAATTGAGATATGGAAAGCAGTTGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTAATGGATATCGCTAGGAGAAACAAGCTTCCAAGGATAATTAGGTATATATTTCTGAAAGGTGCTCGTAGTGTACTCATGTTTAATCTGCATTCTGGAATAATCTTGGCTCATGGTTTGCTTCCCGCCCTCCATCCTCCTGCAGGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTGCTGACATTTTTTCCTGAAGGTTTTTTCTCTTTCGACTTCCATGTAGTTTGTTGATATGCATTTTTCTCCTCATATCATAAGGAGACAAAGATGTCACTTGAATGTATTTGTGTGGCAATTAAAAGTTCATGGTGATATAGGTTGTCTATTTTGTTATATGCTGCAATGTATGGCGAGATTTAATGATTTTTTGTTAACCAGTTGTTCCATTCCATTGCAACAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAAAACAAGCCCATCATCCTATCACATCGTATGCTCTTAAATGCGTTATCAGTTCTTAATCTTGCTGATGCTTGTGATATTAGCAAATAATTGTGATGTCAATGGCGGTTGCAGATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGATGATGAGGTAAGGCATTATAGGGAAAATTGAGCATCCCATGTGTAGAGGAAGTTAGGGTGGTGGTGCAGTTTCGCTAGCATGTAGGTTTGATAGATATAATTAAGAATCCCAGGTGCGGAGGGGAGTAAAATTACATCTTTATTCTTATGATTGTGTTTAGTTATAGGCTGTCTTGACTGAGTGTTCAATTCTGAGGCTATCTTGTAATATTGTGGATCTGTTTTCTAATTTGTTCATCTGGCCATTTTAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCGTTGATCGTGGAAGCGAATCCATGCTTAGAATACATCAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGTAAGTTTGAGGAGATTTATAATCTATTCTTGTTTCTTTCTCCTCCTATATCGTGATCCTAGATGTTAGTTATAATATTGTCGTGACCTCCTATTCATTTATTGTTTTGAAATATTGCAGGACCTTCACAACCTTCGAAGAACTGGTTTCTGACTATGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGGTATGTCATTTTTGCACAATATTTTTCCATGAGTACTTATTGTTATTGGAATTGGTTGTAGTCATCTTTTTGCTTTACTACAGTACAAAACACTATTATGGTTTTTGTGCAAACAATCTTATTAGATCATCATGACTACCTAATTTCAAAATGCAAATTGGTCTATGATGCCGTTGGTAGAGACTAGAAAGCCATAAATAACCATCAATTCAGTACTTTGGGATCTGTCTAGTGCTAAGTCAACCATTGTCTTCTCCTCCTAACACATACGGTTTCTTTGACATTTATTCACGTTCTTGCAATTTCATCTGATAAAACGTTAAACATCTAAGCATAACTTAAGAAGCTTCATATTTTCATGAAAATACAAACAGCTTTTCAGTGAAAAGGGTAGCGATACAGCTAGGAATTTACTTGGTTCTATTTACCCTCAGCTACCTATAAAGCTATGATTGATTTCATTCATCTGCAGCCTGTACGAGATCACTTCAAAAATGATAGCAACGCCAAAGATTTATTGAAGAGGGTCAAGGTATGTGTTCGTTTTACCGGCTCTTAAAGTTATTTGGCTAAATTACAAAGAGTATACTCACTTTGCCATTTGCTTGAAAATACTCATATTTTTCAGAAGTTGTAATAGTACTCTTGCTTCATAATGTATCATGACTACTCTTGGATTAGAATTCCATTAAATTGTTTTTATGAAAATTGGGTACTAGAATGGTCTGGGCACGACCTAGAAAAGAAATTTGGAAAGCCACATTGTTAGGTGGTCACACCATTTCGATTCTCATTTTTCACATAAAGGATTCACACCATGTGTAGTTTTGAAATATGTATGTAAATGGTAATACTGCTACTTTTAGAAGATTTGAGGTTTTATTAAAGTAAAAAGGCAAAGTTTAGTGTAACTTTTTCATCCATTATGCACATGGACCAAATCCCTGGGTTTCCATTGTGGCCTGAACTAAGAAGATTTAAGAACTGCTCATTAGAAATTAGTTCTCAAAATCAAAATGTATGAAATTTTACCTAGAGCTGTTTATCGTGTTATGCAGGCGTACCGAGTCACTAGGTGA

mRNA sequence

ATGGCGACAGAAGCACCTTCGCAAGCTCCATCCGAGGATTTACAGTCCCTCTCAATCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCAAACTCTTCAAACCAATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGATGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGCTATTTAATTGAGATATGGAAAGCAGTTGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTTGTCTATTTTGTTATATGCTGCAATGTATGGCGAGATTTAATGATTTTTTGTTAACCAGTTGTTCCATTCCATTGCAACAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAAAACAAGCCCATCATCCTATCACATCATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGATGATGAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCGTTGATCGTGGAAGCGAATCCATGCTTAGAATACATCAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCACAACCTTCGAAGAACTGGTTTCTGACTATGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGCCTGTACGAGATCACTTCAAAAATGATAGCAACGCCAAAGATTTATTGAAGAGGGTCAAGGCGTACCGAGTCACTAGGTGA

Coding sequence (CDS)

ATGGCGACAGAAGCACCTTCGCAAGCTCCATCCGAGGATTTACAGTCCCTCTCAATCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCAAACTCTTCAAACCAATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGATGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGCTATTTAATTGAGATATGGAAAGCAGTTGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTTGTCTATTTTGTTATATGCTGCAATGTATGGCGAGATTTAATGATTTTTTGTTAACCAGTTGTTCCATTCCATTGCAACAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAAAACAAGCCCATCATCCTATCACATCATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGATGATGAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCGTTGATCGTGGAAGCGAATCCATGCTTAGAATACATCAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCACAACCTTCGAAGAACTGGTTTCTGACTATGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGCCTGTACGAGATCACTTCAAAAATGATAGCAACGCCAAAGATTTATTGAAGAGGGTCAAGGCGTACCGAGTCACTAGGTGA

Protein sequence

MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPLCSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Homology
BLAST of IVF0000871 vs. ExPASy Swiss-Prot
Match: Q8S9J2 (Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g33840 PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.7e-142
Identity = 256/399 (64.16%), Postives = 304/399 (76.19%), Query Frame = 0

Query: 9   APSEDLQSLSISDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKK 68
           A S +++++S+S  +    A SS+   Q+S  +E++Y+IVRS+GEECIQE+EL+NLL KK
Sbjct: 11  ALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAKK 70

Query: 69  PEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIE 128
             P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI 
Sbjct: 71  AAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKIR 130

Query: 129 TVGRYLIEIWKAVG-------WIWKEVRLNFCG---LQRRLTLGQGNTGP---LCSQIMG 188
            VG Y  EIWKA G       ++W    +N          + + + N  P    C QIMG
Sbjct: 131 VVGEYFQEIWKAAGMDNDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQIMG 190

Query: 189 RNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVN 248
           R+E DEL+AAQILYPCMQC    ++                    +ADICQLGMDQRKVN
Sbjct: 191 RSETDELSAAQILYPCMQCADIFFL--------------------EADICQLGMDQRKVN 250

Query: 249 VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAY 308
           VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAY
Sbjct: 251 VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAY 310

Query: 309 CPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDL 368
           CPP +V+ NPCLEYIKY+I PWF EFTVERN ++GGNKT+ +FE++ +DY +GELHPGDL
Sbjct: 311 CPPKVVKGNPCLEYIKYIILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDL 370

Query: 369 KPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           K  L  ALNKIL+PVRDHFK D+ AK+LLK++KAYRVTR
Sbjct: 371 KKGLMNALNKILQPVRDHFKTDARAKNLLKQIKAYRVTR 385

BLAST of IVF0000871 vs. ExPASy Swiss-Prot
Match: F4HWL4 (Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g28350 PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.4e-130
Identity = 243/387 (62.79%), Postives = 283/387 (73.13%), Query Frame = 0

Query: 21  DAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPS 80
           D  +   A SS +  Q+S   E KY+IVRS+GEECIQEDEL+NLL KKP P+CYDGFEPS
Sbjct: 383 DLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPS 442

Query: 81  GRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKA 140
           GRMHIAQGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG Y  EIW+A
Sbjct: 443 GRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQA 502

Query: 141 VG-------WIWKEVRLNFCGLQR-RLTLGQGNTGPL-----CSQIMGRNEQDELTAAQI 200
            G       ++W    +N  G +   L +       L     C QIMGR+E + L+AAQI
Sbjct: 503 GGMNNDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAAQI 562

Query: 201 LYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIKR 260
           LYPCMQC                      I L +ADICQLGMDQRKVN+LAREYC DIKR
Sbjct: 563 LYPCMQC--------------------ADIFLLEADICQLGMDQRKVNMLAREYCADIKR 622

Query: 261 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 320
           KNKPIILSHHMLPGLQQGQEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VE NPCL
Sbjct: 623 KNKPIILSHHMLPGLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCL 682

Query: 321 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 380
           EY+KY++ P F EF VE +  +GGNKTF +FE++V+DY  GELHP DLK AL KALN  L
Sbjct: 683 EYVKYIVLPRFNEFKVE-SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITL 742

Query: 381 EPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           +PVRDHFK +  AK+LL++VKA+RVTR
Sbjct: 743 QPVRDHFKTNERAKNLLEQVKAFRVTR 748

BLAST of IVF0000871 vs. ExPASy Swiss-Prot
Match: Q5UPJ7 (Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.6e-62
Identity = 145/362 (40.06%), Postives = 204/362 (56.35%), Query Frame = 0

Query: 40  EEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSA 99
           E +   + S+ EEC   D L+ L+D       Y+GFEPSGR+HIAQ ++  +N N +   
Sbjct: 10  EHRLTQLLSIAEECETLDRLKQLVDSGRIFTAYNGFEPSGRIHIAQALITVMNTNNIIEC 69

Query: 100 GCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVG---------WIWKEVRLNF 159
           G ++ I+IADWFA++N KM GD+ KI  +GRY IE++KA G         W  + +  N 
Sbjct: 70  GGQMIIYIADWFAKMNLKMNGDINKIRELGRYFIEVFKACGINLDGTRFIWASEFIASNP 129

Query: 160 CGLQRRLTLGQGNT---GPLCSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFND 219
             ++R L + + +T      C QIMGRNE D L A+QI YPCMQ              + 
Sbjct: 130 SYIERMLDIAEFSTISRVKRCCQIMGRNESDCLKASQIFYPCMQAA------------DV 189

Query: 220 FLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKM 279
           F L    I     DICQLG+DQRKVN+LA EY +D +    PI LSHHML  L   ++KM
Sbjct: 190 FELVPEGI-----DICQLGIDQRKVNMLAIEYAND-RGLKIPISLSHHMLMSLSGPKKKM 249

Query: 280 SKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADH 339
           SKSDP  +IFM+D E EV+ KI +AYC     + NP  EYIKYL+  WF    +      
Sbjct: 250 SKSDPQGAIFMDDTEQEVSEKISRAYCTDETFD-NPIFEYIKYLLLRWFGTLNL------ 309

Query: 340 GGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKA 390
              K +T  E +  D+++  ++  +LK  ++  +N I++ VR+HFK      +LL  VK+
Sbjct: 310 -CGKIYTDIESIQEDFSS--MNKRELKTDVANYINTIIDLVREHFKK-PELSELLSNVKS 342

BLAST of IVF0000871 vs. ExPASy Swiss-Prot
Match: Q5JF63 (Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=tyrS PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.2e-47
Identity = 129/402 (32.09%), Postives = 199/402 (49.50%), Query Frame = 0

Query: 37  LSLEEKYQIV-RSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNK 96
           + +E K +++ +   EE + E+ LR+LL+       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIERKIELIKKKPTEELLTEENLRHLLEVGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVG-RYLIEIWKA---------------- 156
           L  AG + +I++ADW + +N+K+GGDL+ I+ V   Y  E  K                 
Sbjct: 61  LQKAGVKTRIFLADWHSWINDKLGGDLEVIQKVALTYFKEGMKQSIKVMGGDPDKVEFVL 120

Query: 157 ------VGWIWKEVRLNFCGLQRRLTLGQGNTGPLCSQIMGRNEQDELTAAQILYPCMQC 216
                  G  W+ V      + + +TL +         IMGR   + +  A+++YP MQ 
Sbjct: 121 ASEILDKGDYWQTV----IDISKNVTLARMLRS---ITIMGRQMGEAIDFAKLIYPAMQV 180

Query: 217 CLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIK-------- 276
               Y                    Q   I   GMDQRK +V+A E    +K        
Sbjct: 181 ADIFY--------------------QGVTIAHAGMDQRKAHVIAIEVAQKLKYHPLEWKG 240

Query: 277 RKNKPIILSHHMLPGLQQ----------------GQEKMSKSDPSSSIFMEDDEAEVNLK 336
            K KP+ L HH+L GLQ+                 Q KMSKS P S++F+ D   E+  K
Sbjct: 241 EKLKPVALHHHLLLGLQEPPVWPIESEEQFKELKTQMKMSKSKPYSAVFIHDSPEEIKQK 300

Query: 337 IKKAYCPPLIVEANPCLEYIKYLIF-PWFKEFTVERNADHGGNKTFTTFEELVSDYAAGE 390
           ++KA+CP   V+ NP L++ +Y+IF     EFT+ R A  GG+ T+TTFEEL  D+A G+
Sbjct: 301 LRKAFCPAREVKYNPVLDWAEYIIFREEPTEFTIHRPAKFGGDVTYTTFEELKKDFAEGK 360

BLAST of IVF0000871 vs. ExPASy Swiss-Prot
Match: C6A0Z6 (Tyrosine--tRNA ligase OS=Thermococcus sibiricus (strain DSM 12597 / MM 739) OX=604354 GN=tyrS PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.5e-47
Identity = 126/398 (31.66%), Postives = 202/398 (50.75%), Query Frame = 0

Query: 37  LSLEEKYQIV-RSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNK 96
           + +E+K ++V R   EE +  D L++LL+       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIEKKIELVSRKPTEEVLTVDNLKDLLEMGMPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVG-----RYLIEIWKAVGWIWKEVRL-- 156
              AG + ++++ADW + +N+K+GGDL+ I+ V        + +  K +G   ++V    
Sbjct: 61  FQKAGIKTRVFLADWHSWINDKLGGDLEVIQKVALGYFKEGMKQSIKVMGGDPEKVEFVL 120

Query: 157 ------------NFCGLQRRLTLGQGNTGPLCSQIMGRNEQDELTAAQILYPCMQCCLFC 216
                           + + +TL +         IMGR   + +  A+++YP MQ     
Sbjct: 121 ASEILERGDYWRTIIDISKNVTLARMMRS---ITIMGRQMGEAIDFAKLIYPAMQVADIF 180

Query: 217 YMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIK--------RKNK 276
           Y                    Q  +I   GMDQRK +V+ARE  + +K        +K K
Sbjct: 181 Y--------------------QGVNIAHAGMDQRKAHVIAREVAEKLKYHPLVWDGKKVK 240

Query: 277 PIILSHHMLPGLQQ----------------GQEKMSKSDPSSSIFMEDDEAEVNLKIKKA 336
           PI + HH+L GLQ+                 Q KMSKS P S++F+ D   E+  K++KA
Sbjct: 241 PIAVHHHLLLGLQEPPKWPIEGEEEFKEIKAQMKMSKSKPYSAVFIHDTPEEIKQKLRKA 300

Query: 337 YCPPLIVEANPCLEYIKYLIF-PWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPG 390
           +CP   V  NP L++ +++IF     EFT+ R A  GG+ T+TTFEEL  D+A G+LHP 
Sbjct: 301 FCPAGEVNYNPVLDWAEHIIFREEPTEFTIHRPAKFGGDVTYTTFEELKRDFAEGKLHPL 360

BLAST of IVF0000871 vs. ExPASy TrEMBL
Match: A0A1S3ATS8 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.3e-188
Identity = 342/407 (84.03%), Postives = 350/407 (86.00%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC    ++                    +ADICQL
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFL--------------------KADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA
Sbjct: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. ExPASy TrEMBL
Match: A0A0A0KMK0 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 8.2e-187
Identity = 338/407 (83.05%), Postives = 349/407 (85.75%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC    ++                    +ADICQL
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFL--------------------KADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAA
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAA 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. ExPASy TrEMBL
Match: A0A5A7THE1 (Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002540 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.0e-184
Identity = 336/401 (83.79%), Postives = 344/401 (85.79%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC    ++                    +ADICQL
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFL--------------------KADICQL 282

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 283 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 342

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA
Sbjct: 343 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 402

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK 387
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK
Sbjct: 403 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK 423

BLAST of IVF0000871 vs. ExPASy TrEMBL
Match: A0A6J1ETE5 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 8.2e-179
Identity = 323/407 (79.36%), Postives = 343/407 (84.28%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPS+DLQSLSI++AKVQEP  SS+SSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +        PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGR EQDELTAAQILYPCMQC    ++                    +ADICQL
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFL--------------------KADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYAS 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           G LHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. ExPASy TrEMBL
Match: A0A6J1HXR2 (Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.8e-178
Identity = 323/407 (79.36%), Postives = 341/407 (83.78%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MAT  PSQAPS+DLQSLSI+DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +        PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGR EQDELTAAQILYPCMQC    ++                    +ADICQL
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFL--------------------KADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYAS 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           G LHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. NCBI nr
Match: XP_008437283.1 (PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo])

HSP 1 Score: 659 bits (1700), Expect = 1.07e-236
Identity = 343/407 (84.28%), Postives = 349/407 (85.75%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC                      I   +ADICQL
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCA--------------------DIFFLKADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA
Sbjct: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 392
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. NCBI nr
Match: XP_004143904.1 (tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical protein Csa_000293 [Cucumis sativus])

HSP 1 Score: 654 bits (1688), Expect = 7.19e-235
Identity = 339/407 (83.29%), Postives = 348/407 (85.50%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC                      I   +ADICQL
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCA--------------------DIFFLKADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAA
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAA 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 392
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. NCBI nr
Match: KAA0042754.1 (tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 648 bits (1671), Expect = 1.41e-231
Identity = 337/401 (84.04%), Postives = 343/401 (85.54%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +    G   PL             
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC                      I   +ADICQL
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCA--------------------DIFFLKADICQL 282

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 283 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 342

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA
Sbjct: 343 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 402

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK 386
           GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK
Sbjct: 403 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK 423

BLAST of IVF0000871 vs. NCBI nr
Match: XP_038906448.1 (tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida])

HSP 1 Score: 639 bits (1647), Expect = 1.26e-228
Identity = 333/407 (81.82%), Postives = 341/407 (83.78%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQA SEDLQSLSISDAKVQEPACSSN SNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQALSEDLQSLSISDAKVQEPACSSNFSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMK INVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +        PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAAEYWPLVMDIARKNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGRNEQDELTAAQILYPCMQC                      I   +ADICQL
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCA--------------------DIFFLKADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTF TFEELV+DYA 
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFATFEELVADYAT 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 392
           G LHPGDLKPALSKALNKILEPVR+HFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GVLHPGDLKPALSKALNKILEPVREHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. NCBI nr
Match: XP_022929135.1 (tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita moschata] >KAG6579509.1 Tyrosine--tRNA ligase 1, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016970.1 Tyrosine--tRNA ligase 1, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628 bits (1619), Expect = 2.31e-224
Identity = 324/407 (79.61%), Postives = 342/407 (84.03%), Query Frame = 0

Query: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPSQAPS+DLQSLSI++AKVQEP  SS+SSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGWIWKEVRLNFCGLQRRLTLGQGNTGPL------------- 180
           DLKKIETVGRYLIEIWKAVG   +  ++ F    + +        PL             
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 --CSQIMGRNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQL 240
             CSQIMGR EQDELTAAQILYPCMQC                      I   +ADICQL
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCA--------------------DIFFLKADICQL 240

Query: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300
           GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV
Sbjct: 241 GMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEV 300

Query: 301 NLKIKKAYCPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAA 360
           NLKIKKAYCPPLIVE NPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+
Sbjct: 301 NLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYAS 360

Query: 361 GELHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 392
           G LHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR
Sbjct: 361 GVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 387

BLAST of IVF0000871 vs. TAIR 10
Match: AT2G33840.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 506.5 bits (1303), Expect = 1.9e-143
Identity = 256/399 (64.16%), Postives = 304/399 (76.19%), Query Frame = 0

Query: 9   APSEDLQSLSISDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKK 68
           A S +++++S+S  +    A SS+   Q+S  +E++Y+IVRS+GEECIQE+EL+NLL KK
Sbjct: 11  ALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAKK 70

Query: 69  PEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIE 128
             P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI 
Sbjct: 71  AAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKIR 130

Query: 129 TVGRYLIEIWKAVG-------WIWKEVRLNFCG---LQRRLTLGQGNTGP---LCSQIMG 188
            VG Y  EIWKA G       ++W    +N          + + + N  P    C QIMG
Sbjct: 131 VVGEYFQEIWKAAGMDNDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQIMG 190

Query: 189 RNEQDELTAAQILYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVN 248
           R+E DEL+AAQILYPCMQC    ++                    +ADICQLGMDQRKVN
Sbjct: 191 RSETDELSAAQILYPCMQCADIFFL--------------------EADICQLGMDQRKVN 250

Query: 249 VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAY 308
           VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAY
Sbjct: 251 VLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAY 310

Query: 309 CPPLIVEANPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDL 368
           CPP +V+ NPCLEYIKY+I PWF EFTVERN ++GGNKT+ +FE++ +DY +GELHPGDL
Sbjct: 311 CPPKVVKGNPCLEYIKYIILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDL 370

Query: 369 KPALSKALNKILEPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           K  L  ALNKIL+PVRDHFK D+ AK+LLK++KAYRVTR
Sbjct: 371 KKGLMNALNKILQPVRDHFKTDARAKNLLKQIKAYRVTR 385

BLAST of IVF0000871 vs. TAIR 10
Match: AT1G28350.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 467.6 bits (1202), Expect = 9.7e-132
Identity = 243/387 (62.79%), Postives = 283/387 (73.13%), Query Frame = 0

Query: 21  DAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPS 80
           D  +   A SS +  Q+S   E KY+IVRS+GEECIQEDEL+NLL KKP P+CYDGFEPS
Sbjct: 383 DLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPS 442

Query: 81  GRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKA 140
           GRMHIAQGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG Y  EIW+A
Sbjct: 443 GRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQA 502

Query: 141 VG-------WIWKEVRLNFCGLQR-RLTLGQGNTGPL-----CSQIMGRNEQDELTAAQI 200
            G       ++W    +N  G +   L +       L     C QIMGR+E + L+AAQI
Sbjct: 503 GGMNNDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAAQI 562

Query: 201 LYPCMQCCLFCYMLQCMARFNDFLLTSCSIPLQQADICQLGMDQRKVNVLAREYCDDIKR 260
           LYPCMQC                      I L +ADICQLGMDQRKVN+LAREYC DIKR
Sbjct: 563 LYPCMQC--------------------ADIFLLEADICQLGMDQRKVNMLAREYCADIKR 622

Query: 261 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 320
           KNKPIILSHHMLPGLQQGQEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VE NPCL
Sbjct: 623 KNKPIILSHHMLPGLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCL 682

Query: 321 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 380
           EY+KY++ P F EF VE +  +GGNKTF +FE++V+DY  GELHP DLK AL KALN  L
Sbjct: 683 EYVKYIVLPRFNEFKVE-SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITL 742

Query: 381 EPVRDHFKNDSNAKDLLKRVKAYRVTR 393
           +PVRDHFK +  AK+LL++VKA+RVTR
Sbjct: 743 QPVRDHFKTNERAKNLLEQVKAFRVTR 748

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S9J22.7e-14264.16Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g3384... [more]
F4HWL41.4e-13062.79Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g2835... [more]
Q5UPJ74.6e-6240.06Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1... [more]
Q5JF634.2e-4732.09Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12... [more]
C6A0Z65.5e-4731.66Tyrosine--tRNA ligase OS=Thermococcus sibiricus (strain DSM 12597 / MM 739) OX=6... [more]
Match NameE-valueIdentityDescription
A0A1S3ATS83.3e-18884.03Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1[more]
A0A0A0KMK08.2e-18783.05Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1[more]
A0A5A7THE11.0e-18483.79Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1ETE58.2e-17979.36Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1[more]
A0A6J1HXR21.8e-17879.36Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008437283.11.07e-23684.28PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo][more]
XP_004143904.17.19e-23583.29tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical ... [more]
KAA0042754.11.41e-23184.04tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa][more]
XP_038906448.11.26e-22881.82tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida][more]
XP_022929135.12.31e-22479.61tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita moschata] >KAG6579509... [more]
Match NameE-valueIdentityDescription
AT2G33840.11.9e-14364.16Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial [more]
AT1G28350.19.7e-13262.79Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 30..152
e-value: 4.2E-54
score: 185.7
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 197..392
e-value: 7.8E-78
score: 263.6
IPR023617Tyrosine-tRNA ligase, archaeal/eukaryotic-typePIRSFPIRSF006588TyrRS_arch_eukcoord: 208..391
e-value: 2.5E-72
score: 241.7
coord: 36..200
e-value: 1.4E-40
score: 137.3
IPR002305Aminoacyl-tRNA synthetase, class IcPFAMPF00579tRNA-synt_1bcoord: 67..366
e-value: 8.8E-45
score: 153.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR46264:SF4TYROSINE-TRNA LIGASEcoord: 15..392
NoneNo IPR availablePANTHERPTHR46264TYROSINE-TRNA LIGASEcoord: 15..392
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 43..377

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000871.1IVF0000871.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004831 tyrosine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding