IVF0000543 (gene) Melon (IVF77) v1

BLAST of IVF0000543 vs. ExPASy Swiss-Prot
Match: Q9M350 (Gamma-tubulin complex component 4 OS=Arabidopsis thaliana OX=3702 GN=GCP4 PE=2 SV=2)

HSP 1 Score: 1055.8 bits (2729), Expect = 2.2e-307
Identity = 559/756 (73.94%), Postives = 629/756 (83.20%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLA DISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRS-GNESSLANSTESSKDKIE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS  +   L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGG 180
           GI EILS+YRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++I EIERDDI+GG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYG+LQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETNQGSSVQDMSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRV 300
           + +  SS +++SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQS--------AGNQ-----SHSNMPRLPLNEPFVATKLTGEELLLQSEAD 360
           LRNPS AF  Q          G+Q      HS+ P     E  +   LTG ELL QSEAD
Sbjct: 301 LRNPSPAFQFQKDKSFQQTMRGSQRIRGFMHSDFPE---TETELDADLTGGELLPQSEAD 360

Query: 361 KIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAK 420
           KIEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL K
Sbjct: 361 KIEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEK 420

Query: 421 GDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVK 480
           GDFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EED+YFSRVSLRMPSFGV 
Sbjct: 421 GDFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVT 480

Query: 481 VKSAQGDL-KEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSK 540
           V+S+Q D+ + K  + G       +NL+ D S+DGWD ++LEY +DWP+QLFFTQEVLSK
Sbjct: 481 VRSSQADMVRSKVSLTG------KANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSK 540

Query: 541 YCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVR 600
           Y +VFQYL+RLKRTQMELEKSWAS+MHQDH + A +R    +GS S QRRQ  R MWRVR
Sbjct: 541 YLKVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVR 600

Query: 601 EHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGS 660
           EHMAFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGS
Sbjct: 601 EHMAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGS 660

Query: 661 VSRILDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRA 720
           VSRILD IMKLCLQFCW+IENQ+S+ + SELE++ EEFNKKSNSLYTILRSS+L GSQRA
Sbjct: 661 VSRILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRA 720

Query: 721 PFLRRFLMRLNFNSFFEATARGVLNVVRPRPAALPI 740
           PFLRRFL+RLNFNSF+EATARGVLNVVR RP ALP+
Sbjct: 721 PFLRRFLLRLNFNSFYEATARGVLNVVRQRP-ALPL 745

BLAST of IVF0000543 vs. ExPASy Swiss-Prot
Match: Q9SC88 (Gamma-tubulin complex component 4 homolog OS=Medicago truncatula OX=3880 GN=85P PE=2 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 5.5e-306
Identity = 550/756 (72.75%), Postives = 627/756 (82.94%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIID R+       NNL  + PIS E TFKLA DISF++PS+R+L
Sbjct: 1   MLHELLLALLGYTGDLIIDRRD-------NNLSANTPISDECTFKLAPDISFIDPSDREL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           I+RII LGFYYREL+RF+ KSRNL+WIRS N + L N         E PSVYRRA+ANGI
Sbjct: 61  IERIITLGFYYRELERFSAKSRNLNWIRSENANPLENK--------EKPSVYRRALANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEIL++Y S++LH+EQ LLSET+PILA VTQGL+KFF L PPL++LI +IER DI+GG+L
Sbjct: 121 VEILAVYSSSILHIEQLLLSETMPILATVTQGLNKFFSLLPPLYELILKIERGDIRGGEL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHK+CHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+L+D+HGEFFI RQ+ R+ 
Sbjct: 181 LNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISRQEGRDV 240

Query: 241 NQGSSVQDMSEKLGRLST-DESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLR 300
              SS Q++SEKL RLST D SL+DWH+GFHI LDMLP+YI MRVAESILFAGKA+RVLR
Sbjct: 241 ENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQ-SAGNQSHSNMPRLP--------LNEPFVATKLTGEELLLQSEADKIEAML 360
           NPS +F  Q     Q     P++           EP + T +  E+LL QSEADKIE ML
Sbjct: 301 NPSPSFLSQDDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVEDLLPQSEADKIENML 360

Query: 361 LDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQC 420
           LDLKESSEFHKRSFE AVDSI+AIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQC
Sbjct: 361 LDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQC 420

Query: 421 FLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSAQG 480
           FLEESR LMRLPPRQSTAEADLM+PFQLA+ KT  EED+YFS+VSLRMPS+G+ VK +  
Sbjct: 421 FLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKPSLL 480

Query: 481 DLKE--KPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           ++ +      DG  G ++ SN S +MS+DGWDG++LEY I+WPL LFFTQEVLS+Y +VF
Sbjct: 481 NVPKATSAAADGISGASI-SNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLKVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGS-ISLQRRQRFRRMWRVREHMA 600
           QYLLRLKRTQMELEK WAS+MHQ H+ FA N+ +  D S I+ QR QRFR MWRVREHMA
Sbjct: 541 QYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWNILQ HIQDSHDFTELVGFHQEYLSALISQ+FLDIGSVSRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LDGIMKLCLQFCW+IENQD+ S+ SELEH+ EEFNKKSNSLYTILRSSRL GSQR PFLR
Sbjct: 661 LDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           RFL+RLN NSFFE+TA+ V+NVVRPRP   P LNQ+
Sbjct: 721 RFLLRLNLNSFFESTAKEVMNVVRPRP-TFPGLNQR 739

BLAST of IVF0000543 vs. ExPASy Swiss-Prot
Match: Q9UGJ1 (Gamma-tubulin complex component 4 OS=Homo sapiens OX=9606 GN=TUBGCP4 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.3e-85
Identity = 227/738 (30.76%), Postives = 365/738 (49.46%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           + R+  LG  Y     F  +                +   S + +     +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEFIEQYT--------GHVQQQDHHPSQQGQGGLHGIYLRAFCTGL 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
             +L  YR A+L +EQ+ L +    ++ V   LD+F +LFP +  ++ +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++GLL D+H EFFI +Q     
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFI-KQGPSSG 240

Query: 241 NQGSSVQDMSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMRV 300
           N  +  ++  E LG               RL  +E++    L  F + +++LP YI +RV
Sbjct: 241 NVSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRV 300

Query: 301 AESILFAGKAIRVLRNPSNAFWCQSAGNQSHSNMPRLPLNEPFVATKLTGEELLLQSEAD 360
           AE ILF G+++++  N +                             LT +  +L+++ D
Sbjct: 301 AEKILFVGESVQMFENQN---------------------------VNLTRKGSILKNQED 360

Query: 361 KIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAK 420
              A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G LK +KD++LL +
Sbjct: 361 TFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGR 420

Query: 421 GDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVK 480
           G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D     + L +   G +
Sbjct: 421 GELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKE 480

Query: 481 VKSAQGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKY 540
            K+     +E P  + +P  A +S         GW  + L Y + WPL + FT  VL KY
Sbjct: 481 HKADATQAREGPSRETSPREAPAS---------GWAALGLSYKVQWPLHILFTPAVLEKY 540

Query: 541 CRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVRE 600
             VF+YLL ++R Q EL+  WA  M + H                L+  Q     WR+R 
Sbjct: 541 NVVFKYLLSVRRVQAELQHCWALQMQRKH----------------LKSNQTDAIKWRLRN 600

Query: 601 HMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSV 660
           HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L++QSF+ +  V
Sbjct: 601 HMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPV 655

Query: 661 SRILDGIMKLCLQFCWSIENQDSSSD---PSELEHLTEEFNKKSNSLYTILRSSRLVGSQ 720
              L+ I+ LC  FC  +       D    ++L  L + F+++S+ L+ IL S R    Q
Sbjct: 661 FHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILSSVR--NHQ 655

BLAST of IVF0000543 vs. ExPASy Swiss-Prot
Match: Q9D4F8 (Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 7.4e-85
Identity = 226/738 (30.62%), Postives = 366/738 (49.59%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           + R+  LG  Y     F      +       +    +  +  +  +    +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEF------IEQYTGHVQQQDHHPPQQGQGGLH--GIYLRAFCTGL 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
             +L  YR A+L +EQ+ L++    ++ V   LD+F +LFP +  ++ +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++GLL D+H EFFI +Q     
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFI-KQGPSSG 240

Query: 241 NQGSSVQDMSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMRV 300
              + +++  E LG               RL  +E++    L  F + +++LP YI +RV
Sbjct: 241 TLSAQLEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRV 300

Query: 301 AESILFAGKAIRVLRNPSNAFWCQSAGNQSHSNMPRLPLNEPFVATKLTGEELLLQSEAD 360
           AE ILF G+++++  N +                             LT +  +L+++ D
Sbjct: 301 AEKILFVGESVQMFENQN---------------------------VNLTRKGSILKNQED 360

Query: 361 KIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAK 420
              A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G LK +KD++LL +
Sbjct: 361 TFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGR 420

Query: 421 GDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVK 480
           G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D     + L +   G  
Sbjct: 421 GELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKD 480

Query: 481 VKSAQGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKY 540
            K+     +E P  + +P  A SS         GW  + L Y + WPL + FT  VL KY
Sbjct: 481 HKADATQPREVPSRETSPREAPSS---------GWAALGLSYKVQWPLHILFTPAVLEKY 540

Query: 541 CRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVRE 600
             VF+YLL ++R Q EL+  WA  M + H                L+  Q     WR+R 
Sbjct: 541 NVVFKYLLSVRRVQAELQHCWALQMQRKH----------------LKSNQTDAVKWRLRN 600

Query: 601 HMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSV 660
           HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L++QSF+ +  V
Sbjct: 601 HMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPV 655

Query: 661 SRILDGIMKLCLQFCWSIENQDSSSD---PSELEHLTEEFNKKSNSLYTILRSSRLVGSQ 720
              L+ I+ LC  FC  +       D    ++L  L + F+++S+ L+ IL S R    Q
Sbjct: 661 FHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILSSVR--NHQ 655

BLAST of IVF0000543 vs. ExPASy Swiss-Prot
Match: Q9VKU7 (Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster OX=7227 GN=Grip75 PE=1 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 3.6e-39
Identity = 170/692 (24.57%), Postives = 304/692 (43.93%), Query Frame = 0

Query: 52  FLEPSERDLIQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSV 111
           F+ P ER++   II +   Y+E+++F   S      +S     L +S             
Sbjct: 31  FIHPCEREIFMDIIKIIKVYQEVEQFTHSSGR----KSDTHGELPDSLH---------GY 90

Query: 112 YRRAIANGIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIE 171
           Y   +A GI   L  Y + +  +E+  L      L+ V   L   F L   +  LI EI 
Sbjct: 91  YLLNLAKGIEMALEEYYAEIGRLEKYCLGNERNSLSYVYNALYAKFPLLVFMRNLITEIH 150

Query: 172 RDDIQGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFF 231
             +++G  LL+ LH++C  G  +L+  I+ ++       ++ LA W+++G++ D H EFF
Sbjct: 151 VLNLRGCVLLHNLHQQCEHGDIQLEKAIKIIMKPVKNAFFSSLAHWLLFGVIDDVHSEFF 210

Query: 232 IRRQDDRETNQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFA 291
           I +    +   GSS    S     LS +++  D+   + + +  LP +  + +AE +LF 
Sbjct: 211 I-KFTPTDAVDGSSF-SKSATCSLLSAEKNPEDYIWQYEVNMSQLPGFFSIVLAEKVLFV 270

Query: 292 GKAIRVLRNPSNAFWCQSAGNQSHSNMPRLPLNEPFVATKLTGEEL--LLQSEADKIEAM 351
           G+ + V +   N        N++     +L         +L  +++  L      +   M
Sbjct: 271 GQTVLVFKMGRNV----KVKNKTDPLAAKL--------AELDSDDIYQLWSGRESEFFKM 330

Query: 352 LLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQ 411
           ++DL      +    E  +  I+   ++ L ++ V   DL   +  +KD+FLL +G+F+ 
Sbjct: 331 VVDLSNEDTINVFRLEKVIIDIKNYVSARLSEIAVNEVDLERQMGLIKDFFLLGRGEFYL 390

Query: 412 CFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED-RYFSRVSLRMPSFGVKVKSA 471
            F   S+ +  +   +     ++   F+LAAT T   +D   FS +  R  S        
Sbjct: 391 EFC--SQMVGTMETYREERFKNVTRSFELAATVTGITDDLDKFSLICQRSTS-------- 450

Query: 472 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 531
                        P      N           G+SL+Y  +WPL L F+   + +Y  +F
Sbjct: 451 ------------EPDDTSDFNF--------LQGLSLKYEYEWPLNLLFSPTTIERYNNIF 510

Query: 532 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 591
           ++LL ++  Q E+++ WA                Q   + S +      ++  +R ++ F
Sbjct: 511 RFLLIIRTYQYEIQRVWAK---------------QTWRAKSAKDVPPNNKIITLRNYLMF 570

Query: 592 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFL--------- 651
            + N+Q+YIQVDV+ESQ+ IL + I+   DF  +   H  +L+ ++S  FL         
Sbjct: 571 FLNNMQYYIQVDVLESQFGILMNVIKSRSDFEVIQRAHTVFLANVLSHCFLLNESETQLN 630

Query: 652 DIGSVSR--ILDGIMKL---CLQFCWSIENQDSSSD-PSELEHLTEEFNKKSNSLYTIL- 711
             GS +R  I   ++KL   C +F    + +D S D   E++ L E F  +  SL  +L 
Sbjct: 631 VTGSQNRNPIYGTLLKLFGICEKFAHMTQTKDPSDDLEDEVDQLNESFGVQIASLIQLLV 645

Query: 712 --RSSRLVGSQRAPFLRRFLMRLNFNSFFEAT 723
             +S+  +G      L + L+RL+FN +F A+
Sbjct: 691 DVKSASCLGP-----LSQLLLRLDFNCWFSAS 645

BLAST of IVF0000543 vs. ExPASy TrEMBL
Match: A0A5A7SW23 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00060 PE=3 SV=1)

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 743/755 (98.41%), Postives = 743/755 (98.41%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQSAGNQSHSNMPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of IVF0000543 vs. ExPASy TrEMBL
Match: A0A5A7VFR5 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00280 PE=3 SV=1)

HSP 1 Score: 1441.4 bits (3730), Expect = 0.0e+00
Identity = 735/755 (97.35%), Postives = 738/755 (97.75%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLA STESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLI EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSNMPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALSSNLSLDMS DGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFAN RNAQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           FLMRLNFNSFFEATARGVLNVVRPRP+ALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of IVF0000543 vs. ExPASy TrEMBL
Match: A0A6J1F2V2 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111441869 PE=3 SV=1)

HSP 1 Score: 1367.4 bits (3538), Expect = 0.0e+00
Identity = 697/755 (92.32%), Postives = 715/755 (94.70%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS N SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPL------------NEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPL             EPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEK   DGNPGGAL SNLS DMSLDGWD V+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDPKEKTYTDGNPGGAL-SNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+ NRNA+FDGSIS Q+RQRFR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           FLMRLNFNSFFEATARGVLNVV PRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of IVF0000543 vs. ExPASy TrEMBL
Match: A0A6J1J9A7 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111482469 PE=3 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 691/755 (91.52%), Postives = 714/755 (94.57%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGLN+L  DAPISGEPTFKLA DISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS + SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPL------------NEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPL             EPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEKP  DGNPGGAL SNLS DMSLDGW+GV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDPKEKPYTDGNPGGAL-SNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+ NRNA+FDGSIS Q+RQ FR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQ+ SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           FLMRLNFNSFFEATARGVLNVVR RPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 754

BLAST of IVF0000543 vs. ExPASy TrEMBL
Match: A0A6J1DP06 (Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111022444 PE=3 SV=1)

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 693/755 (91.79%), Postives = 711/755 (94.17%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLN+L  DAPISGE TFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLPPDAPISGESTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN SSLA S ESSK K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLAKSAESSKAKTENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQD HGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDNHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
            QGSSVQDMSEKLGRLSTD+SLTDWHLGFHIYLDMLPDYIHM VAESILFAGKAIRVLRN
Sbjct: 241 EQGSSVQDMSEKLGRLSTDDSLTDWHLGFHIYLDMLPDYIHMWVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AG QSHSN+ R+PLN            EPF+ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGYQSHSNISRVPLNIKGNTRNFPLQKEPFIATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKE+SEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKEASEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGV+VKS 
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVRVKST 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           Q    EK   DGNPG AL SNLS DMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 Q----EKTYTDGNPGAAL-SNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFA NR AQ DGS+S  +RQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFAQNRRAQRDGSVSSHQRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFT+LVG+HQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTDLVGYHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSS+PSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSNPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 744
           FLMRLNFNSFFEATARGVLNVVR RPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRARPAALPVLNQQ 750

BLAST of IVF0000543 vs. NCBI nr
Match: KAA0035230.1 (gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1462 bits (3785), Expect = 0.0
Identity = 743/755 (98.41%), Postives = 743/755 (98.41%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQSAGNQSHSNMPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 743
           FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of IVF0000543 vs. NCBI nr
Match: KAA0067222.1 (gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1445 bits (3740), Expect = 0.0
Identity = 735/755 (97.35%), Postives = 738/755 (97.75%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLA STESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLI EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSNMPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALSSNLSLDMS DGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFAN RNAQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 743
           FLMRLNFNSFFEATARGVLNVVRPRP+ALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of IVF0000543 vs. NCBI nr
Match: XP_011660150.1 (gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_011660151.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_031744625.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >KGN66813.2 hypothetical protein Csa_007116 [Cucumis sativus])

HSP 1 Score: 1444 bits (3738), Expect = 0.0
Identity = 735/755 (97.35%), Postives = 738/755 (97.75%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL+NLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSE VPILAIVTQGLDKFFVLFPPLHQLIREIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSEMVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSNMPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYF RVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFCRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALS NLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALS-NLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNR AQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 743
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of IVF0000543 vs. NCBI nr
Match: XP_038879485.1 (gamma-tubulin complex component 4 homolog [Benincasa hispida])

HSP 1 Score: 1408 bits (3645), Expect = 0.0
Identity = 716/755 (94.83%), Postives = 728/755 (96.42%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLL+LLGYTGDLIIDEREHYNSLGLN+L +DAPISGEPTF LA DISFLEPSERDL
Sbjct: 1   MLHELLLSLLGYTGDLIIDEREHYNSLGLNHLPLDAPISGEPTFTLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN SSLANSTESSKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKF+VLFPPLHQLI EIE DDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFYVLFPPLHQLILEIEHDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD+SEKLGRLSTDESLTDWHLGFHI LDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDVSEKLGRLSTDESLTDWHLGFHICLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPLN            EPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEF KRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFRKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEKP IDGNPGGALS NL LDMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDPKEKPFIDGNPGGALS-NLPLDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSIS Q+RQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISPQQRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWS+ENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSLENQDGSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 743
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of IVF0000543 vs. NCBI nr
Match: XP_022934806.1 (gamma-tubulin complex component 4 homolog [Cucurbita moschata] >XP_022934807.1 gamma-tubulin complex component 4 homolog [Cucurbita moschata])

HSP 1 Score: 1370 bits (3547), Expect = 0.0
Identity = 697/755 (92.32%), Postives = 715/755 (94.70%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS N SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQSAGNQSHSNMPRLPLN------------EPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPL             EPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEK   DGNPGGALS NLS DMSLDGWD V+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDPKEKTYTDGNPGGALS-NLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+ NRNA+FDGSIS Q+RQRFR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 743
           FLMRLNFNSFFEATARGVLNVV PRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of IVF0000543 vs. TAIR 10
Match: AT3G53760.1 (GAMMA-TUBULIN COMPLEX PROTEIN 4 )

HSP 1 Score: 1055.8 bits (2729), Expect = 1.6e-308
Identity = 559/756 (73.94%), Postives = 629/756 (83.20%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLA DISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRS-GNESSLANSTESSKDKIE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS  +   L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGG 180
           GI EILS+YRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++I EIERDDI+GG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYG+LQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETNQGSSVQDMSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRV 300
           + +  SS +++SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQS--------AGNQ-----SHSNMPRLPLNEPFVATKLTGEELLLQSEAD 360
           LRNPS AF  Q          G+Q      HS+ P     E  +   LTG ELL QSEAD
Sbjct: 301 LRNPSPAFQFQKDKSFQQTMRGSQRIRGFMHSDFPE---TETELDADLTGGELLPQSEAD 360

Query: 361 KIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAK 420
           KIEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL K
Sbjct: 361 KIEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEK 420

Query: 421 GDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVK 480
           GDFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EED+YFSRVSLRMPSFGV 
Sbjct: 421 GDFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVT 480

Query: 481 VKSAQGDL-KEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSK 540
           V+S+Q D+ + K  + G       +NL+ D S+DGWD ++LEY +DWP+QLFFTQEVLSK
Sbjct: 481 VRSSQADMVRSKVSLTG------KANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSK 540

Query: 541 YCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVR 600
           Y +VFQYL+RLKRTQMELEKSWAS+MHQDH + A +R    +GS S QRRQ  R MWRVR
Sbjct: 541 YLKVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVR 600

Query: 601 EHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGS 660
           EHMAFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGS
Sbjct: 601 EHMAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGS 660

Query: 661 VSRILDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRA 720
           VSRILD IMKLCLQFCW+IENQ+S+ + SELE++ EEFNKKSNSLYTILRSS+L GSQRA
Sbjct: 661 VSRILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRA 720

Query: 721 PFLRRFLMRLNFNSFFEATARGVLNVVRPRPAALPI 740
           PFLRRFL+RLNFNSF+EATARGVLNVVR RP ALP+
Sbjct: 721 PFLRRFLLRLNFNSFYEATARGVLNVVRQRP-ALPL 745

BLAST of IVF0000543 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 112.8 bits (281), Expect = 1.2e-24
Identity = 116/466 (24.89%), Postives = 192/466 (41.20%), Query Frame = 0

Query: 175 IQGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRR 234
           ++GG +   +H     G P +   +  LL      ++  + SW++ G L+D  GEFF+  
Sbjct: 334 LRGGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVG 393

Query: 235 QDDRETNQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKA 294
           Q                K+  L        W  G+ ++  MLP +I   +A+ IL  GK+
Sbjct: 394 Q--------------PVKVDLL--------WREGYKLHPAMLPSFISPSLAQRILRTGKS 453

Query: 295 IRVLRNPSNAF-WCQSAGNQSHSNMPRLPLNEPFVATKLTGEELLLQSEADKIEAMLLDL 354
           I  LR   +   W  +A   + ++            T+  G   L   E D +E ++   
Sbjct: 454 INFLRVCCDDHGWADAASEAAAAS---------GTTTRRGG---LGYGETDALEHLV--- 513

Query: 355 KESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLE 414
              +E  KR     +D        HL  ++  R     H  A+K Y LL +GDF Q    
Sbjct: 514 ---TEAAKR-----ID-------KHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQ---- 573

Query: 415 ESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSAQGDLK 474
              +LM +   + +  A+ +  F+LA           F   ++R  +     +     L+
Sbjct: 574 ---YLMDIVGPKLSEPANNISSFELAG----------FLEAAIRASNAQYDDRDMLDRLR 633

Query: 475 EKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQYLLR 534
            K +  G+                GWD  SLEY    PL   FT+ VLSKY RVF +L +
Sbjct: 634 VKMMPHGSGD-------------RGWDVFSLEYEARVPLDTVFTESVLSKYLRVFNFLWK 693

Query: 535 LKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAFLIRNL 594
           LKR +  L   W ++         +N   +   S+ LQ     RR   +   M   + N 
Sbjct: 694 LKRVEHALIGIWKTMKPN---CITSNSFVKLQSSVKLQLLSALRRCQVLWNEMNHFVTNF 714

Query: 595 QFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFL 640
           Q+YI  +V+E  W+     ++ + D  +L+  H++YL+A++ +S L
Sbjct: 754 QYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLL 714

BLAST of IVF0000543 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 101.7 bits (252), Expect = 2.7e-21
Identity = 123/564 (21.81%), Postives = 231/564 (40.96%), Query Frame = 0

Query: 163 LHQLIREIERDDIQGGQLLNLLHK--RCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVY 222
           L  +I++       G  +LNLL    +   G   +++ ++++         + L  W+  
Sbjct: 190 LAAVIQQASTKQFVGSGVLNLLQSQAKAMAGDNSVRSLLEKMTECASNAYLSILERWVYE 249

Query: 223 GLLQDKHGEFFIRRQDDRETNQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYI 282
           G++ D +GEFFI              ++ S K   LS D +   W   + +  D +P ++
Sbjct: 250 GIIDDPYGEFFI-------------AENRSLKKESLSQDSTAKYWSQRYSL-KDTIPGFL 309

Query: 283 HMRVAESILFAGKAIRVLRNPSNAFWCQSAGNQSHSNMPRLPLNEPFVATKLTGEELLLQ 342
              +A +IL  GK + V+R       C         +  ++P++E    +KLT    +  
Sbjct: 310 -ANIAATILTTGKYLNVMRE------C--------GHNVQVPISE---RSKLT----IFG 369

Query: 343 SEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYF 402
           S    +E     +K + EF               A+  L  L+  + DL G L+++K Y 
Sbjct: 370 SNHHYLEC----IKAAHEF---------------ASIELVNLIKDKYDLVGRLRSIKHYL 429

Query: 403 LLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPS 462
           LL +GDF   F++ +R  +     + + E  L     LA   T +  D     ++  +  
Sbjct: 430 LLDQGDFLVHFMDIAREELNKKVHEISVE-KLQSLLDLALRTTAAAADPRHEDLTCCVDR 489

Query: 463 FGVKVKSAQGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEV 522
               + +  G  K+            S+++   MS+ G +  SL Y + WPL +  +++ 
Sbjct: 490 --ASLLTTLGMHKDTD----------SNSIEDPMSITGLETFSLSYKVQWPLSIVISKKA 549

Query: 523 LSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMW 582
           LSKY  +F++L   K  + +L  +W   +HQ      +   A    S+  +   +F    
Sbjct: 550 LSKYQLIFRFLFHCKHVERQLCGAWQ--IHQGIRSMNSKGTAILRSSLLCRSMLKF---- 609

Query: 583 RVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLD 642
                    I +L  Y+  +V+E  W+++ D +Q +    E++  H  +L   +    L 
Sbjct: 610 ---------ISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLL 666

Query: 643 IGSVSRILDGIMKLCLQFC----WSIENQ---DSSSDPSE-----------LEHLTEEFN 702
           +  V + ++ +  +CLQ+     W I +    +S S P +           + +   EFN
Sbjct: 670 LPDVLKKMEKLKSVCLQYAAATQWLISSSIDINSQSHPQKTMIRDTTVTESIFNFEREFN 666

Query: 703 KKSNSLYTILRSSRLVGSQRAPFL 707
            +  SL  +L      GSQ  P+L
Sbjct: 730 SELQSLGPVLSK----GSQAEPYL 666

BLAST of IVF0000543 vs. TAIR 10
Match: AT5G17410.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 101.7 bits (252), Expect = 2.7e-21
Identity = 123/564 (21.81%), Postives = 231/564 (40.96%), Query Frame = 0

Query: 163 LHQLIREIERDDIQGGQLLNLLHK--RCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVY 222
           L  +I++       G  +LNLL    +   G   +++ ++++         + L  W+  
Sbjct: 189 LAAVIQQASTKQFVGSGVLNLLQSQAKAMAGDNSVRSLLEKMTECASNAYLSILERWVYE 248

Query: 223 GLLQDKHGEFFIRRQDDRETNQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYI 282
           G++ D +GEFFI              ++ S K   LS D +   W   + +  D +P ++
Sbjct: 249 GIIDDPYGEFFI-------------AENRSLKKESLSQDSTAKYWSQRYSL-KDTIPGFL 308

Query: 283 HMRVAESILFAGKAIRVLRNPSNAFWCQSAGNQSHSNMPRLPLNEPFVATKLTGEELLLQ 342
              +A +IL  GK + V+R       C         +  ++P++E    +KLT    +  
Sbjct: 309 -ANIAATILTTGKYLNVMRE------C--------GHNVQVPISE---RSKLT----IFG 368

Query: 343 SEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYF 402
           S    +E     +K + EF               A+  L  L+  + DL G L+++K Y 
Sbjct: 369 SNHHYLEC----IKAAHEF---------------ASIELVNLIKDKYDLVGRLRSIKHYL 428

Query: 403 LLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPS 462
           LL +GDF   F++ +R  +     + + E  L     LA   T +  D     ++  +  
Sbjct: 429 LLDQGDFLVHFMDIAREELNKKVHEISVE-KLQSLLDLALRTTAAAADPRHEDLTCCVDR 488

Query: 463 FGVKVKSAQGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEV 522
               + +  G  K+            S+++   MS+ G +  SL Y + WPL +  +++ 
Sbjct: 489 --ASLLTTLGMHKDTD----------SNSIEDPMSITGLETFSLSYKVQWPLSIVISKKA 548

Query: 523 LSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMW 582
           LSKY  +F++L   K  + +L  +W   +HQ      +   A    S+  +   +F    
Sbjct: 549 LSKYQLIFRFLFHCKHVERQLCGAWQ--IHQGIRSMNSKGTAILRSSLLCRSMLKF---- 608

Query: 583 RVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLD 642
                    I +L  Y+  +V+E  W+++ D +Q +    E++  H  +L   +    L 
Sbjct: 609 ---------ISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLL 665

Query: 643 IGSVSRILDGIMKLCLQFC----WSIENQ---DSSSDPSE-----------LEHLTEEFN 702
           +  V + ++ +  +CLQ+     W I +    +S S P +           + +   EFN
Sbjct: 669 LPDVLKKMEKLKSVCLQYAAATQWLISSSIDINSQSHPQKTMIRDTTVTESIFNFEREFN 665

Query: 703 KKSNSLYTILRSSRLVGSQRAPFL 707
            +  SL  +L      GSQ  P+L
Sbjct: 729 SELQSLGPVLSK----GSQAEPYL 665

BLAST of IVF0000543 vs. TAIR 10
Match: AT3G43610.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 51.2 bits (121), Expect = 4.1e-06
Identity = 72/348 (20.69%), Postives = 129/348 (37.07%), Query Frame = 0

Query: 389  LNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED 448
            L  HL AL+ Y  +   D+   F+    H   L        A++    + +  +++ E D
Sbjct: 879  LQEHLLALRRYHFMELADWADVFVVSLWHHKWLVTEADKRIAEIQGFLESSIQRSSCERD 938

Query: 449  RYFSRVSLRMPSFGVKVKSAQGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYI 508
                R+ L            QG +   P   G               +  +D + L Y +
Sbjct: 939  ICKDRIFL---------YKRQGTMHIPPSTIG---------------VRSFDFLRLGYRV 998

Query: 509  DWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSI 568
            DWP+ +  T + L+ Y  VF +L+++K     L   W SL    H              I
Sbjct: 999  DWPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLKDVRHMMHEKKE------KI 1058

Query: 569  SLQRRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDS-HDFTELVGFHQ 628
              Q  +    + ++R  +   +  LQ Y+  ++    W+     +++   D  +L   H 
Sbjct: 1059 LKQELRWLNILMKLRHQVNHFVTALQQYVHSELSHVSWSKFLHSLKNKVKDMMDLESVHM 1118

Query: 629  EYLSALISQSFL--DIGSVSRILDGIMKLCLQFCWSIENQDSSSD--------------P 688
             YLS  +   FL  +   +S I++ I++  L F   +     S+D               
Sbjct: 1119 AYLSEALRICFLSDETQIISNIIENILQCALDFRSCLPRGIQSTDRVPNDSWTKTLGINT 1178

Query: 689  SELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFLMRLNFNSFF 720
            S++  + + F+K+   L+     S   G      L RF   LNFN ++
Sbjct: 1179 SQVMMVKQNFDKELKELHKCHLRSPKHGKYG---LSRFWDYLNFNLYY 1193