HG10023191 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023191 vs. NCBI nr
Match: XP_038900248.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 792.0 bits (2044), Expect = 2.9e-225
Identity = 413/479 (86.22%), Postives = 417/479 (87.06%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGS-GGDGGEPSNRPKRLS 60
           MA SISGLSLPSVF+Y+SSILARKPRF SFSIRSS DVSGEVGS GGDG EPSNRPKRLS
Sbjct: 1   MAFSISGLSLPSVFQYESSILARKPRFRSFSIRSSNDVSGEVGSGGGDGSEPSNRPKRLS 60

Query: 61  EQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKV 120
           EQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKV
Sbjct: 61  EQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKV 120

Query: 121 TRSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIF 180
           TRSF HLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIF
Sbjct: 121 TRSFGHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIF 180

Query: 181 QAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHA 240
           QAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                   
Sbjct: 181 QAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ------------------- 240

Query: 241 EQLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNN 300
                                            GKLSCLMINDIDAGIGRFGSTQVTVNN
Sbjct: 241 ---------------------------------GKLSCLMINDIDAGIGRFGSTQVTVNN 300

Query: 301 QIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQ 360
           QIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQ
Sbjct: 301 QIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQ 360

Query: 361 PNREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGG 420
           PNREDIVNIVHRMYEKDGISRAQV DIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGG
Sbjct: 361 PNREDIVNIVHRMYEKDGISRAQVADIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGG 420

Query: 421 VEKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           VEKLGEKLLKRRKNEKLP F PPEQTL+ALLEAGYSLV EQ+LIMETKLSKEYMKNMDD
Sbjct: 421 VEKLGEKLLKRRKNEKLPTFVPPEQTLEALLEAGYSLVKEQKLIMETKLSKEYMKNMDD 427

BLAST of HG10023191 vs. NCBI nr
Match: XP_008461213.1 (PREDICTED: ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucumis melo] >KAA0058685.1 ribulose bisphosphate carboxylase/oxygenase activase [Cucumis melo var. makuwa])

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-222
Identity = 404/478 (84.52%), Postives = 413/478 (86.40%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPSVFRYKSSILARKPRF SFS+RSS DVS EVGSGGDG + S RPKRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAP FMDKVVCHIVKNF+VHLLNVKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
            MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 MMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVSVGQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRKNEKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. NCBI nr
Match: TYK10491.1 (ribulose bisphosphate carboxylase/oxygenase activase [Cucumis melo var. makuwa])

HSP 1 Score: 781.9 bits (2018), Expect = 3.0e-222
Identity = 403/478 (84.31%), Postives = 413/478 (86.40%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPSVFRYKSSILARKPRF SFS+RSS DVS EVGSGGDG + S RPKRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAP FMDKVVCHIVKNF+VHLLNVKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
            MGVEPVIMSAGELES+RAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 MMGVEPVIMSAGELESDRAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVSVGQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRKNEKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. NCBI nr
Match: XP_004135891.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucumis sativus] >KGN45193.1 hypothetical protein Csa_016801 [Cucumis sativus])

HSP 1 Score: 774.2 bits (1998), Expect = 6.3e-220
Identity = 394/478 (82.43%), Postives = 414/478 (86.61%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPS+ RYKSSIL RKPRFPSFS+RSS DVSGE+GSGGDG E + RPKRLSE
Sbjct: 1   MAFSISGLSLPSILRYKSSILPRKPRFPSFSVRSSNDVSGEIGSGGDGSESAKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QS+WEAKDSEGKDYLYRLG+EADNMNIAVGARAGVIDD+FAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDVFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           R+FEH+QGDYYIAP FMDKVVCH+VKNF+VHLL+VKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RTFEHIQGDYYIAPTFMDKVVCHLVKNFIVHLLDVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
           AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVS+GQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSIGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRK+EKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKSEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. NCBI nr
Match: XP_022960536.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 750.4 bits (1936), Expect = 9.8e-213
Identity = 384/478 (80.33%), Postives = 408/478 (85.36%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA++ISG+SLPS+F YK+SI+ARK R  SFSIRS KDVSGE G GG GGEPS +PKRLSE
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGE-GDGG-GGEPSQKPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           +S+WEAKDSEGKDYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYRQKVT
Sbjct: 61  KSSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAPVFMDKVVCHIVKNF+VHLLN+KVPLILG+WGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
           AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GKLSCLMINDIDAGIGRF +TQVTVNNQ
Sbjct: 241 --------------------------------GKLSCLMINDIDAGIGRFENTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IV+GTLMNLADNP RVS+GQDWREADI NRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQV+DIVN FPNQALDFYGALRSRTYD +ISKWVDDIGG+
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKWVDDIGGM 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLLKRRKNE+LP FTPPEQT+D LLEAGYSL+ EQQ +METKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQFVMETKLSKEYMKNMDD 424

BLAST of HG10023191 vs. ExPASy Swiss-Prot
Match: P93431 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RCA PE=1 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 1.6e-85
Identity = 167/355 (47.04%), Postives = 221/355 (62.25%), Query Frame = 0

Query: 120 TRSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIF 179
           T  F++  G +YIAP FMDK+V HI KNFM  L N+KVPLILG+WGGKGQGKSFQ EL+F
Sbjct: 112 TYDFDNTMGGFYIAPAFMDKLVVHISKNFMT-LPNIKVPLILGIWGGKGQGKSFQCELVF 171

Query: 180 QAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHA 239
             MG+ P++MSAGELES  AGEP KLIR+RYR A+ +++                     
Sbjct: 172 AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKK-------------------- 231

Query: 240 EQLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-GSTQVTVN 299
                                            GK+ CL IND+DAG GR  G+TQ TVN
Sbjct: 232 ---------------------------------GKMCCLFINDLDAGAGRMGGTTQYTVN 291

Query: 300 NQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYW 359
           NQ+V+ TLMN+ADNPT V +   + + D   R+PII+TGNDFST+YAPLIRDGRMEKFYW
Sbjct: 292 NQMVNATLMNIADNPTNVQLPGMYNKED-NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYW 351

Query: 360 QPNREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIG 419
            P R+D V +   ++  D +    +V IV+ FP Q++DF+GALR+R YD  + KWV D  
Sbjct: 352 APTRDDRVGVCKGIFRTDNVPDEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDT- 408

Query: 420 GVEKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYM 474
           GVE +G++L+  R  E  P F  P+ T++ L+E GY LV EQ+ +   +L+++Y+
Sbjct: 412 GVENIGKRLVNSR--EGPPEFEQPKMTIEKLMEYGYMLVKEQENVKRVQLAEQYL 408

BLAST of HG10023191 vs. ExPASy Swiss-Prot
Match: O49074 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Solanum pennellii OX=28526 PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.8e-84
Identity = 201/483 (41.61%), Postives = 269/483 (55.69%), Query Frame = 0

Query: 1   MALSIS--GLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRL 60
           MA S+S  G +  +     +S+        +F  +S K V      G    + SNR  R+
Sbjct: 1   MAASVSTIGAASKAPLSLNNSVAGTSVPSTAFFGKSLKKV---YAKGVSSPKVSNRNLRV 60

Query: 61  SEQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLF-AGNFLGKDSDIVFDYRQ 120
             Q   E K+   K  LY      D  +IA G   G++D LF A    G    I+  Y +
Sbjct: 61  VAQEVDETKEDRWKG-LYD-NTSDDQQDIARG--KGLVDSLFQAPTGTGTHHAIMNSY-E 120

Query: 121 KVTRSFEHLQGD-----YYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKS 180
            V+++ +  Q D     +YIAP FMDK+V HI KNF+  L N+KVPLILGVWGGKGQGKS
Sbjct: 121 YVSQALKTYQLDNKLDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGVWGGKGQGKS 180

Query: 181 FQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYV 240
           FQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++++              
Sbjct: 181 FQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRK------------- 240

Query: 241 FTTYVHAEQLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-G 300
                                                   G + CL IND+DAG GR  G
Sbjct: 241 ----------------------------------------GNMCCLFINDLDAGAGRMGG 300

Query: 301 STQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDG 360
           +TQ TVNNQ+V+ TLMN+ADNPT V +   + + +   R+PII+TGNDFST+YAPLIRDG
Sbjct: 301 TTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENA-RVPIIVTGNDFSTLYAPLIRDG 360

Query: 361 RMEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAIS 420
           RMEKFYW P RED + +   ++  D +    VV IV+ FP Q++DF+GALR+R YD  + 
Sbjct: 361 RMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVVKIVDSFPGQSIDFFGALRARVYDDEVR 417

Query: 421 KWVDDIGGVEKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKE 475
           KWV    G+E +GEKLL  R     P F  P+ TL+ LLE G  LV EQ+ +   +L++ 
Sbjct: 421 KWVSGT-GIELIGEKLLNSRDGP--PTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLAET 417

BLAST of HG10023191 vs. ExPASy Swiss-Prot
Match: Q9ZT00 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Zea mays OX=4577 GN=RCA1 PE=2 SV=3)

HSP 1 Score: 313.9 bits (803), Expect = 3.1e-84
Identity = 191/478 (39.96%), Postives = 269/478 (56.28%), Query Frame = 0

Query: 6   SGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSEQSTWE 65
           S +  P+    +SS L +K   P         VS  V   G     ++R K ++ +   E
Sbjct: 7   STVGAPASTPTRSSFLGKKLNKP--------QVSAAVTYHGKSSSSNSRFKAMAAKEVDE 66

Query: 66  AKDSE---GKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIV----FDYRQK 125
            K ++    K   Y + +  D  +I  G   G++D+LF    +G  + +     +DY  +
Sbjct: 67  TKQTDEDRWKGLAYDISD--DQQDITRG--KGLVDNLFQAP-MGDGTHVAVLSSYDYISQ 126

Query: 126 VTRS--FEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTE 185
             +S  F+++   +YIA  FMDK+V H+ KNFM  L N+KVPLILG+WGGKGQGKSFQ E
Sbjct: 127 GQKSYNFDNMMDGFYIAKGFMDKLVVHLSKNFMT-LPNIKVPLILGIWGGKGQGKSFQCE 186

Query: 186 LIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTY 245
           L+F  MG+ P++MSAGELES  AGEP KLIR+RYR AS +++                  
Sbjct: 187 LVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREASDLIKK----------------- 246

Query: 246 VHAEQLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-GSTQV 305
                                               GK+SCL IND+DAG GR  G+TQ 
Sbjct: 247 ------------------------------------GKMSCLFINDLDAGAGRMGGTTQY 306

Query: 306 TVNNQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEK 365
           TVNNQ+V+ TLMN+ADNPT V +   + + D   R+PII+TGNDFST+YAPLIRDGRMEK
Sbjct: 307 TVNNQMVNATLMNIADNPTNVQLPGMYNKED-NPRVPIIVTGNDFSTLYAPLIRDGRMEK 366

Query: 366 FYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVD 425
           FYW P RED + +   ++  DG+    VV +V+ FP Q++DF+GALR+R YD  + +WV 
Sbjct: 367 FYWAPTREDRIGVCKGIFRTDGVDEEHVVQLVDTFPGQSIDFFGALRARVYDDEVRRWVS 413

Query: 426 DIGGVEKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYM 474
           +  GVE +  KL+  +  E  P F  P+ T++ LLE G+ LV EQ+ +   +L+ +Y+
Sbjct: 427 ET-GVENIARKLVNSK--EGPPTFEQPKITIEKLLEYGHMLVAEQENVKRVQLADKYL 413

BLAST of HG10023191 vs. ExPASy Swiss-Prot
Match: Q40460 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 9.9e-83
Identity = 177/426 (41.55%), Postives = 246/426 (57.75%), Query Frame = 0

Query: 55  PKRLSEQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLF-AGNFLGKDSDIV- 114
           PK+ ++   W+    +  D         D  +I  G   G++D LF A    G    ++ 
Sbjct: 66  PKKQTDSDRWKGLVQDFSD---------DQQDITRG--KGMVDSLFQAPTGTGTHHAVLQ 125

Query: 115 -FDYRQKVTRSF--EHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQ 174
            ++Y  +  R +  ++    +YIAP FMDK+V HI KNF+  L N+KVPLILG+WGGKGQ
Sbjct: 126 SYEYVSQGLRQYNLDNKLDGFYIAPAFMDKLVVHITKNFL-KLPNIKVPLILGIWGGKGQ 185

Query: 175 GKSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHE 234
           GKSFQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++++           
Sbjct: 186 GKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRK---------- 245

Query: 235 VYVFTTYVHAEQLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGR 294
                                                      G + CL IND+DAG GR
Sbjct: 246 -------------------------------------------GNMCCLFINDLDAGAGR 305

Query: 295 F-GSTQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLI 354
             G+TQ TVNNQ+V+ TLMN+ADNPT V +   + + +   R+PII+TGNDFST+YAPLI
Sbjct: 306 MGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENA-RVPIIVTGNDFSTLYAPLI 365

Query: 355 RDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDA 414
           RDGRMEKFYW P RED + +   ++  D +    VV IV+ FP Q++DF+GALR+R YD 
Sbjct: 366 RDGRMEKFYWAPTREDRIGVCTGIFRTDNVPAEDVVKIVDNFPGQSIDFFGALRARVYDD 422

Query: 415 AISKWVDDIGGVEKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKL 474
            + KWV    G+EK+G+KLL     +  P F  P+ T++ LLE G  LV EQ+ +   +L
Sbjct: 426 EVRKWVSGT-GIEKIGDKLL--NSFDGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQL 422

BLAST of HG10023191 vs. ExPASy Swiss-Prot
Match: Q7X999 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA2 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 9.9e-83
Identity = 167/386 (43.26%), Postives = 230/386 (59.59%), Query Frame = 0

Query: 94  GVIDDLFAGNFLGKDSDIV---FDYRQKVTRSF--EHLQGDYYIAPVFMDKVVCHIVKNF 153
           G++D LF           V   +DY  +  R +  ++    +YIAP FMDK+V HI KNF
Sbjct: 90  GMVDTLFQAPMQSGTHYAVMSSYDYISQGLRQYNLDNNMDGFYIAPAFMDKLVVHITKNF 149

Query: 154 MVHLLNVKVPLILGVWGGKGQGKSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRE 213
           +  L N+K+PLILG+WGGKGQGKSFQ EL+F  MG+ P++MSAGELES  AGEP KLIR+
Sbjct: 150 L-SLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQ 209

Query: 214 RYRTASQVVQNQTYTRTVGHEVYVFTTYVHAEQLCVQLVMFPIRAVQKFISNRIDFENVI 273
           RYR A+ +++                                                  
Sbjct: 210 RYREAADIIKK------------------------------------------------- 269

Query: 274 PFVWGKLSCLMINDIDAGIGRF-GSTQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADI 333
               GK+ CL IND+DAG GR  G+TQ TVNNQ+V+ TLMN+ADNPT V +   + + + 
Sbjct: 270 ----GKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE- 329

Query: 334 LNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIV 393
             R+PII+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D +    +V +V
Sbjct: 330 NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVV 389

Query: 394 NIFPNQALDFYGALRSRTYDAAISKWVDDIGGVEKLGEKLLKRRKNEKLPAFTPPEQTLD 453
           + FP Q++DF+GALR+R YD  + KWV ++ GV+ +G+KL+  +  E  P F  P+ T+D
Sbjct: 390 DQFPGQSIDFFGALRARVYDDEVRKWVSEV-GVDTIGKKLVNSK--EGPPTFEQPKMTID 417

Query: 454 ALLEAGYSLVTEQQLIMETKLSKEYM 474
            LL+ G  LV EQ+ +   +L+ +YM
Sbjct: 450 KLLQYGNMLVEEQENVKRVQLADKYM 417

BLAST of HG10023191 vs. ExPASy TrEMBL
Match: A0A5A7UYP6 (Ribulose bisphosphate carboxylase/oxygenase activase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001550 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.6e-223
Identity = 404/478 (84.52%), Postives = 413/478 (86.40%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPSVFRYKSSILARKPRF SFS+RSS DVS EVGSGGDG + S RPKRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAP FMDKVVCHIVKNF+VHLLNVKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
            MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 MMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVSVGQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRKNEKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. ExPASy TrEMBL
Match: A0A1S3CFE2 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499863 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.6e-223
Identity = 404/478 (84.52%), Postives = 413/478 (86.40%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPSVFRYKSSILARKPRF SFS+RSS DVS EVGSGGDG + S RPKRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAP FMDKVVCHIVKNF+VHLLNVKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
            MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 MMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVSVGQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRKNEKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. ExPASy TrEMBL
Match: A0A5D3CFD7 (Ribulose bisphosphate carboxylase/oxygenase activase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001410 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.5e-222
Identity = 403/478 (84.31%), Postives = 413/478 (86.40%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPSVFRYKSSILARKPRF SFS+RSS DVS EVGSGGDG + S RPKRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAP FMDKVVCHIVKNF+VHLLNVKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKVVCHIVKNFIVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
            MGVEPVIMSAGELES+RAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 MMGVEPVIMSAGELESDRAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVSVGQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRKNEKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. ExPASy TrEMBL
Match: A0A0A0K6Q9 (ATPase_AAA_core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430770 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.1e-220
Identity = 394/478 (82.43%), Postives = 414/478 (86.61%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA SISGLSLPS+ RYKSSIL RKPRFPSFS+RSS DVSGE+GSGGDG E + RPKRLSE
Sbjct: 1   MAFSISGLSLPSILRYKSSILPRKPRFPSFSVRSSNDVSGEIGSGGDGSESAKRPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           QS+WEAKDSEGKDYLYRLG+EADNMNIAVGARAGVIDD+FAGNFLGKDSDIVFDYRQKVT
Sbjct: 61  QSSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDVFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           R+FEH+QGDYYIAP FMDKVVCH+VKNF+VHLL+VKVPLILGVWGGKGQGKSFQTELIFQ
Sbjct: 121 RTFEHIQGDYYIAPTFMDKVVCHLVKNFIVHLLDVKVPLILGVWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
           AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GK+SCLMINDIDAGIGRFG TQVTVNNQ
Sbjct: 241 --------------------------------GKMSCLMINDIDAGIGRFGQTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IVSGTLMNLADNPTRVS+GQDWREADIL+RIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVSGTLMNLADNPTRVSIGQDWREADILHRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQVVDIVN FPNQALDFYGALRSRTYD+AISKWVDDIGGV
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGALRSRTYDSAISKWVDDIGGV 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLL+RRK+EKLP FTPPEQTL+ALL+AGYSLV EQQLIMETKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLRRRKSEKLPTFTPPEQTLEALLKAGYSLVKEQQLIMETKLSKEYMKNMDD 426

BLAST of HG10023191 vs. ExPASy TrEMBL
Match: A0A6J1H7W0 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461246 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.8e-213
Identity = 384/478 (80.33%), Postives = 408/478 (85.36%), Query Frame = 0

Query: 1   MALSISGLSLPSVFRYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSE 60
           MA++ISG+SLPS+F YK+SI+ARK R  SFSIRS KDVSGE G GG GGEPS +PKRLSE
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGE-GDGG-GGEPSQKPKRLSE 60

Query: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120
           +S+WEAKDSEGKDYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYRQKVT
Sbjct: 61  KSSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVT 120

Query: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQ 180
           RSFEHLQGDYYIAPVFMDKVVCHIVKNF+VHLLN+KVPLILG+WGGKGQGKSFQTELIFQ
Sbjct: 121 RSFEHLQGDYYIAPVFMDKVVCHIVKNFIVHLLNIKVPLILGIWGGKGQGKSFQTELIFQ 180

Query: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAE 240
           AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ                    
Sbjct: 181 AMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQ-------------------- 240

Query: 241 QLCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQ 300
                                           GKLSCLMINDIDAGIGRF +TQVTVNNQ
Sbjct: 241 --------------------------------GKLSCLMINDIDAGIGRFENTQVTVNNQ 300

Query: 301 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360
           IV+GTLMNLADNP RVS+GQDWREADI NRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP
Sbjct: 301 IVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 360

Query: 361 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 420
           NREDIVNIVHRMYEKDGISRAQV+DIVN FPNQALDFYGALRSRTYD +ISKWVDDIGG+
Sbjct: 361 NREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALRSRTYDTSISKWVDDIGGM 420

Query: 421 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           EKLGEKLLKRRKNE+LP FTPPEQT+D LLEAGYSL+ EQQ +METKLSKEYMKNMDD
Sbjct: 421 EKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQFVMETKLSKEYMKNMDD 424

BLAST of HG10023191 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 602.4 bits (1552), Expect = 3.1e-172
Identity = 308/464 (66.38%), Postives = 359/464 (77.37%), Query Frame = 0

Query: 15  RYKSSILARKPRFPSFSIRSSKDVSGEVGSGGDGGEPSNRPKRLSEQSTWEAKDSEGKDY 74
           R  SSI+  KP        SS D  G+V +   G +P    K+LSEQS+WE KDSEGKDY
Sbjct: 33  RKLSSIVCSKP--------SSND-GGKVANDDGGAKPR---KKLSEQSSWEVKDSEGKDY 92

Query: 75  LYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVTRSFEHLQGDYYIAP 134
           LYRLG E+DN+NIAVGAR+G+IDD+F G+FLGKDSDIVFDYRQK TRSFEHLQGDYYIAP
Sbjct: 93  LYRLGAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIVFDYRQKATRSFEHLQGDYYIAP 152

Query: 135 VFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQAMGVEPVIMSAGEL 194
            F+DKV  HIVKN++   LN+K+PLILG+WGGKGQGK+FQTELIF+ MGVEPVIMSAGEL
Sbjct: 153 SFLDKVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGVEPVIMSAGEL 212

Query: 195 ESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAEQLCVQLVMFPIRAV 254
           ES+RAGEPG+LIR+RYRTASQV+QNQ                                  
Sbjct: 213 ESDRAGEPGRLIRDRYRTASQVIQNQ---------------------------------- 272

Query: 255 QKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRFGSTQVTVNNQIVSGTLMNLADNPT 314
                             GK+S LMINDIDAG+GRFG TQ+TVNNQIV GTLMNLADNPT
Sbjct: 273 ------------------GKMSVLMINDIDAGLGRFGETQMTVNNQIVVGTLMNLADNPT 332

Query: 315 RVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYE 374
           RVSVGQ+WREAD++NR+P+I+TGNDFST+YAPLIR+GRMEKFYWQP REDIVNIV RMYE
Sbjct: 333 RVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEKFYWQPTREDIVNIVSRMYE 392

Query: 375 KDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGVEKLGEKLLKRRKNE 434
           KDGISR  V+ IV+ FPNQALDFYGALRSRTYD +I KWVD+ GG+E LG+ LL+R+K +
Sbjct: 393 KDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVDEAGGMETLGKVLLRRKKTQ 432

Query: 435 KLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYMKNMDD 479
           ++P FT PEQT++ALLE+GYSL+ EQ+LIMETKLSKEYMKNMDD
Sbjct: 453 EVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYMKNMDD 432

BLAST of HG10023191 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 160/353 (45.33%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 122 SFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQA 181
           + +++   +YIAP FMDK+V HI KNF+  L N+KVPLILG+WGGKGQGKSFQ EL+   
Sbjct: 123 NLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIWGGKGQGKSFQCELVMAK 182

Query: 182 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAEQ 241
           MG+ P++MSAGELES  AGEP KLIR+RYR A+ +++                       
Sbjct: 183 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKK---------------------- 242

Query: 242 LCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-GSTQVTVNNQ 301
                                          GK+ CL IND+DAG GR  G+TQ TVNNQ
Sbjct: 243 -------------------------------GKMCCLFINDLDAGAGRMGGTTQYTVNNQ 302

Query: 302 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 361
           +V+ TLMN+ADNPT V +   + + +   R+PII TGNDFST+YAPLIRDGRMEKFYW P
Sbjct: 303 MVNATLMNIADNPTNVQLPGMYNKEENA-RVPIICTGNDFSTLYAPLIRDGRMEKFYWAP 362

Query: 362 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 421
            RED + +   ++  D I    +V +V+ FP Q++DF+GALR+R YD  + K+V+ + GV
Sbjct: 363 TREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GV 417

Query: 422 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYM 474
           EK+G++L+  R  E  P F  PE T + L+E G  LV EQ+ +   +L++ Y+
Sbjct: 423 EKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYL 417

BLAST of HG10023191 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 160/353 (45.33%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 122 SFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQA 181
           + +++   +YIAP FMDK+V HI KNF+  L N+KVPLILG+WGGKGQGKSFQ EL+   
Sbjct: 123 NLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIWGGKGQGKSFQCELVMAK 182

Query: 182 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAEQ 241
           MG+ P++MSAGELES  AGEP KLIR+RYR A+ +++                       
Sbjct: 183 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKK---------------------- 242

Query: 242 LCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-GSTQVTVNNQ 301
                                          GK+ CL IND+DAG GR  G+TQ TVNNQ
Sbjct: 243 -------------------------------GKMCCLFINDLDAGAGRMGGTTQYTVNNQ 302

Query: 302 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 361
           +V+ TLMN+ADNPT V +   + + +   R+PII TGNDFST+YAPLIRDGRMEKFYW P
Sbjct: 303 MVNATLMNIADNPTNVQLPGMYNKEENA-RVPIICTGNDFSTLYAPLIRDGRMEKFYWAP 362

Query: 362 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 421
            RED + +   ++  D I    +V +V+ FP Q++DF+GALR+R YD  + K+V+ + GV
Sbjct: 363 TREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GV 417

Query: 422 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYM 474
           EK+G++L+  R  E  P F  PE T + L+E G  LV EQ+ +   +L++ Y+
Sbjct: 423 EKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYL 417

BLAST of HG10023191 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 160/353 (45.33%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 122 SFEHLQGDYYIAPVFMDKVVCHIVKNFMVHLLNVKVPLILGVWGGKGQGKSFQTELIFQA 181
           + +++   +YIAP FMDK+V HI KNF+  L N+KVPLILG+WGGKGQGKSFQ EL+   
Sbjct: 123 NLDNMMDGFYIAPAFMDKLVVHITKNFLT-LPNIKVPLILGIWGGKGQGKSFQCELVMAK 182

Query: 182 MGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQTYTRTVGHEVYVFTTYVHAEQ 241
           MG+ P++MSAGELES  AGEP KLIR+RYR A+ +++                       
Sbjct: 183 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKK---------------------- 242

Query: 242 LCVQLVMFPIRAVQKFISNRIDFENVIPFVWGKLSCLMINDIDAGIGRF-GSTQVTVNNQ 301
                                          GK+ CL IND+DAG GR  G+TQ TVNNQ
Sbjct: 243 -------------------------------GKMCCLFINDLDAGAGRMGGTTQYTVNNQ 302

Query: 302 IVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQP 361
           +V+ TLMN+ADNPT V +   + + +   R+PII TGNDFST+YAPLIRDGRMEKFYW P
Sbjct: 303 MVNATLMNIADNPTNVQLPGMYNKEENA-RVPIICTGNDFSTLYAPLIRDGRMEKFYWAP 362

Query: 362 NREDIVNIVHRMYEKDGISRAQVVDIVNIFPNQALDFYGALRSRTYDAAISKWVDDIGGV 421
            RED + +   ++  D I    +V +V+ FP Q++DF+GALR+R YD  + K+V+ + GV
Sbjct: 363 TREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GV 417

Query: 422 EKLGEKLLKRRKNEKLPAFTPPEQTLDALLEAGYSLVTEQQLIMETKLSKEYM 474
           EK+G++L+  R  E  P F  PE T + L+E G  LV EQ+ +   +L++ Y+
Sbjct: 423 EKIGKRLVNSR--EGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYL 417