HG10022635 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022635
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
LocationChr05: 26470928 .. 26473617 (-)
RNA-Seq ExpressionHG10022635
SyntenyHG10022635
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTCCTCAACATCTTCTCCATGGCCGTCCCTCGAACCAGAACGCAATCAGTCCTCAAAACGCTTCGTCACATGAGCAATATCCCCGAAAACTCGGTCTACGGCGGCCCCCAGTCGCGGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAGAGCTGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCAGCAGTCCATTTGGAGATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTAGTACACGGCCACGACACCACCTTGCCTATTAGCTTAGATGAGATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGATCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCTGTACAGGTTATTTCCCTTTTGCCTTTTACGTGTTTGAAGAAATGACAGCTATCTGGTTTTATTCATTTGAAAGTTAGCGAAAGTCTGTTTTTTGTCGTTCTTTGAATGTACTGTACAGGAATCTCAGGGATTCATTAGATTGTAAAATGTAAAAGCAATTCCAAGCTCTTGTGAGAATCTCTACTTTTACTTGCTGAATAGGAGTTATTATCTAAACGATTGAATATGGGAGGTCTTCAGTTCATATCAAAAAATGATTTCATTTAGTTTCTTGGTTACATTAGGTCATCTTTATGTTTCAACATGCATAAACAGACTGAACTTACTTGATTGGTTTTTTATTTCTATTGTTTCTTTTTACGATCATCACTTGGTTTAAGTGCTCCACTTTTCCATTAGAGAGTCTTTTGTGACTCCTTTCAAGTGGGGTTTTTACCCTTTTCTTTGTAATTTCATATCATCAATAAAATTGATTCTCATATTGATTATTATTTTGGTAACTCAGATGAAAGCTAGTTTAGAAATGTAAGAACTTATCTTTGATGGGAAACTTGAGTGGGATTTTTTGCCTGTAATTGAATCGTACGAGCAAGCTTTTTTTTTAGGATGTTAAAACCTTAAACAAATCAATTCATTCCTACTTGACGAACACCTCAAATTTTTAAAATGATTCAGAACTGTGTTGCTATTATTGACAGGCTGTTGATACGGCAGTAAAAATTCTGAAGGACGGGGGAATGGATGCAATAAAATTGGAAGGGGGCGCACATTCTAGAATCACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGATCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTTCCCCACTGCTCTATTTGTCTCTTCCTCTTAGTGTACTGTTTGCAGCTGTTTCTGGGACACCATCTCTGTTGTTATGTTGCTTAGTTTGTCGTGTTTGTTTTGGCTTTTATGCTGAATAGTGCATCTTTGCAGGTGGTGGAAACTGCACTATCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAACTACTGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTCATTGCACAGACATCCTTATTTATGCTATCTGTACATACATACATTCATATATGTGTGTGTGTGTGTGTGTGACTGCCAGCCGTATTTCCTTTCCTTGAGCTGTTTGTTTGTTTCTCTTTGTTTGATCTGGCATATAATTATACCTTCAAACTATTTCTTTACCCCTGAGTTCAGGAGGCAGAGGTTCTCTCCCAAATTCCCATTGAGAGAAGCATCAAAGGTTGTTCTTGTTTAGGATTCCAAAATGGTCCTCTTTTATGGTTCTTATTCTAGCACAATGAAATTGGAACAGAGTGGATTGAAACTGTTTAAATTGTAGATTCCCTAAGGGAGTGGATGCATTAGAACATAGAAGACATTCAAGAGTAGTGAAATATTTCTACTGGTAAACAGTACACTTGCTATCAAGTCATAGAGGCAAAATAGCAATTTGTTAATATTTGGGTTCGAATGCTGCTCCCTCATGTCGGAAGTAGCTTATGAAGAAGAAAACAGACCTTGCCTTTCAGTCAATAAAGAGACACGGTTTATGATTTATTCAATATTGCAGGCAACTTCTTGGTTTTTCATTTTTTGTTCAGAAATGTTGATTTCATCTTCTATACTTGTTTGATAAGGTTGTTCGTTGTGTTGCTCGTGTTGTCTCTGATTATTTGACAACAACCATAGTGTTGAATATCCTCTGCAGTTGGACTAATTAATTTCTTTAACTCAGGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTTACCATCAGGCTGCACCTCTGTTTCGTCTAAGTTTCTCTATGTTTGTTTAAGGTGTTTTGACTCCTGGATTGTATAATATGCCCTTTGCAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCGAACTGGAGAAGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCGCCCAATAGCCCATATACGATTAGTGATGACGATTTCAGTGGTTTCTTGAAAGAGTTACAAAACATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATCTTGA

mRNA sequence

ATGGCGCTCCTCAACATCTTCTCCATGGCCGTCCCTCGAACCAGAACGCAATCAGTCCTCAAAACGCTTCGTCACATGAGCAATATCCCCGAAAACTCGGTCTACGGCGGCCCCCAGTCGCGGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAGAGCTGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCAGCAGTCCATTTGGAGATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTAGTACACGGCCACGACACCACCTTGCCTATTAGCTTAGATGAGATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGATCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCTGTACAGGCTGTTGATACGGCAGTAAAAATTCTGAAGGACGGGGGAATGGATGCAATAAAATTGGAAGGGGGCGCACATTCTAGAATCACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGATCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTGGTGGAAACTGCACTATCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAACTACTGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCGAACTGGAGAAGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCGCCCAATAGCCCATATACGATTAGTGATGACGATTTCAGTGGTTTCTTGAAAGAGTTACAAAACATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATCTTGA

Coding sequence (CDS)

ATGGCGCTCCTCAACATCTTCTCCATGGCCGTCCCTCGAACCAGAACGCAATCAGTCCTCAAAACGCTTCGTCACATGAGCAATATCCCCGAAAACTCGGTCTACGGCGGCCCCCAGTCGCGGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAGAGCTGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCAGCAGTCCATTTGGAGATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTAGTACACGGCCACGACACCACCTTGCCTATTAGCTTAGATGAGATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGATCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCTGTACAGGCTGTTGATACGGCAGTAAAAATTCTGAAGGACGGGGGAATGGATGCAATAAAATTGGAAGGGGGCGCACATTCTAGAATCACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGATCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTGGTGGAAACTGCACTATCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAACTACTGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCGAACTGGAGAAGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCGCCCAATAGCCCATATACGATTAGTGATGACGATTTCAGTGGTTTCTTGAAAGAGTTACAAAACATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATCTTGA

Protein sequence

MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANRS
Homology
[Close]
BLAST of HG10022635 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 610.9 bits (1574), Expect = 6.8e-171
Identity = 308/347 (88.76%), Postives = 332/347 (95.68%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MA+ N+FS A  RTRT+ +LKTLR+MSN+PEN+VYGGP S++PN RVTLTNLRQKYK+ E
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSAAMVVHG+DTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAV++LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA+SLQEAGCFAVVLECVPPEVAAA T+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKALS+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSEYRE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISD DF+GFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 308/347 (88.76%), Postives = 331/347 (95.39%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL  + +MAV RTRTQS+LKTLR+MSN+PEN+VYGGP S++PNQRVTLTNLRQKYK+ E
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSAAMVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAV+ILK+GGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNI SAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISD DF+GFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. NCBI nr
Match: XP_008447278.1 (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 601.7 bits (1550), Expect = 4.1e-168
Identity = 306/347 (88.18%), Postives = 326/347 (93.95%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL  + +MA  RTR QS+LKTLR+MSN+PEN+VY GP S++PNQRVTLTNLRQKYK+ E
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSAAMVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QAVDTAV+ILK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNI SAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP +SPY ISD D +GFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599.4 bits (1544), Expect = 2.0e-167
Identity = 303/347 (87.32%), Postives = 329/347 (94.81%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL N+ + A  R+ +QS+L TLR MSN+PEN+VYGGP S +PNQRVTLTNL+QKYK+ E
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAV++LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 237 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 296

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 297 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 356

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKALSDYKE
Sbjct: 357 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 416

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISDDD++GFLKEL++MGLD AASAAAAKANR
Sbjct: 417 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

[Close]
BLAST of HG10022635 vs. NCBI nr
Match: XP_022953648.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita moschata] >XP_023547697.1 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.4 bits (1544), Expect = 2.0e-167
Identity = 303/347 (87.32%), Postives = 329/347 (94.81%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL N+ + A  R+ +QS+L TLR MSN+PEN+VYGGP S +PNQRVTLTNL+QKYK+ E
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAV++LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISDDD++GFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.2e-148
Identity = 261/334 (78.14%), Postives = 300/334 (89.82%), Query Frame = 0 

Query: 14  TRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSA 73
           +R    + T+R MSN+PE++VYGGP+ ++ NQRVTLT LRQK+++ EPITMVTAYDYPSA
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH++ AGID+ LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAV++LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPF 253
           GGFR QGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAA T+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A+ GEVINKAL +YKEEVS   FPGP +S
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           PY IS  D  GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 4.9e-148
Identity = 260/324 (80.25%), Postives = 297/324 (91.67%), Query Frame = 0 

Query: 22  TLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSAVHLEMAGI 81
           ++R MSN+PEN+VYGGP+ ++PNQRVTLT+LRQK++R EPIT+VTAYDYPSAVHL+ AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRSQGK 201
           DTAV++LK+GGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFR QG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAA T+AL+IPT+GIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNSPYTISDDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A  GEVINKAL +YKEEVS   FPGP +SPY I+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 FSGFLKELQNMGLDEAASAAAAKA 346
             GFL ELQ +G D+AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

[Close]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.8e-135
Identity = 246/337 (73.00%), Postives = 283/337 (83.98%), Query Frame = 0 

Query: 24  RHMSNIPENSVYGGPQSR------------SPNQRVTLTNLRQKYKRAEPITMVTAYDYP 83
           R +S++PE++VYGGP+ +               +RVT+T LR K++R EPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFG 143
           SAVH++ AGID+ LVGDSAAMVVHGHDTTLPISLD ML HCRAVARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AV++LK+GGMDAIKLEGGA SRI+AA+AIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAG 263
           VLGGFR QGK + SAVKVVETAL+LQEAGCF+VVLECVP  VAAA T+AL+IPT+GIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPP 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF   G VINKALS+YK+EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 NSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANRS 349
           ++PY I+  D  GF   LQ MGLDEAA+AAAA A  +
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAAENA 354

[Close]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 4.6e-114
Identity = 215/326 (65.95%), Postives = 257/326 (78.83%), Query Frame = 0 

Query: 24  RHMSN-IPENSVYGG------PQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSAVHL 83
           R MSN   E ++YGG        S +  +RVTL  LR K++R EPI+MVTAYDYPS VH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  EMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTYESS 143
           + AG DI LVGDSAAMV HGHD TLPISLD M+ HCRAVARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGF 203
           + QAV +AV+++K+GG+++IKLEG A SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPFCSG 263
           R QGK + SAVKVVE AL+LQEAGCFAVVLECVP  VAAA T+AL IPT+GIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNSPYT 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+   G+VIN+ALS YK+EV   SFPGP ++PY 
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISDDDFSGFLKELQNMGLDEAASAAA 343
           ++  D   FL  L+  GL+ AA AAA
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAA 378

[Close]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.5e-69
Identity = 132/269 (49.07%), Postives = 192/269 (71.38%), Query Frame = 0 

Query: 45  QRVTLTNLRQKYKRAEPITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPIS 104
           +++TL   ++  K+ + + M+TAYDYP A  ++ + I+I LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHS 164
           LDEM+ HC+ V RGA   L++ D+PFG+Y  S   A+  A ++LK+ G++A+K+EGG   
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAARAIVEAGIAVMGHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAV 224
             T  RA+V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E AL+L+ AG F++
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPEVAAATTAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPT+GIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 ARTGEVINKALSDYKEEVSNGSFPGPPNS 314
           A+TG  I  AL+DY  EV++G FPGP +S
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

[Close]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.3e-171
Identity = 308/347 (88.76%), Postives = 331/347 (95.39%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL  + +MAV RTRTQS+LKTLR+MSN+PEN+VYGGP S++PNQRVTLTNLRQKYK+ E
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSAAMVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAV+ILK+GGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNI SAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISD DF+GFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-168
Identity = 306/347 (88.18%), Postives = 326/347 (93.95%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL  + +MA  RTR QS+LKTLR+MSN+PEN+VY GP S++PNQRVTLTNLRQKYK+ E
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSAAMVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QAVDTAV+ILK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNI SAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP +SPY ISD D +GFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.9e-168
Identity = 303/347 (87.32%), Postives = 329/347 (94.81%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL N+ + A  R+ +QS+L TLR MSN+PEN+VYGGP S +PNQRVTLTNL+QKYK+ E
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAV++LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISDDD++GFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 302/347 (87.03%), Postives = 330/347 (95.10%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           MAL N+ + A  R+ +QS+L TLR+MSN+PEN+VYGGP S +PNQRVTLTNL+QKYK+ E
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPS VHL+MAGIDIALVGDSA+MVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAV++LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA+SLQEAGCFAVVLECVPPEVAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGP +SPY ISD+D++GFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match: A0A6J1D0E3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111016428 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.8e-165
Identity = 297/348 (85.34%), Postives = 326/348 (93.68%), Query Frame = 0 

Query: 1   MALLNIFSMAVPRTRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAE 60
           M L N F++A  RTRT+S+LKTLR+MSN+PEN+VYGGP+ + P QRVTLTNLRQK+K+ E
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITMVTAYDYPSAVHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+ VHL+MAGIDIALVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAV+ILK+GGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAA 240
           GHVGLTPQAISVLGGFR QGKNIASAVKVVETA++LQEAGCFAVVLECVPP VAAA TAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA+ G+VIN+AL DYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVSNGSFPGPPNSPYTISDDDFSGFLKELQNMGLDEAASAAAAKANRS 349
           EV+NGSFPGP +SPY IS+DD++GFLKELQN+GLDEAASAAAA    S
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSS 348

[Close]
BLAST of HG10022635 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 261/334 (78.14%), Postives = 300/334 (89.82%), Query Frame = 0 

Query: 14  TRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSA 73
           +R    + T+R MSN+PE++VYGGP+ ++ NQRVTLT LRQK+++ EPITMVTAYDYPSA
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH++ AGID+ LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAV++LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPF 253
           GGFR QGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAA T+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A+ GEVINKAL +YKEEVS   FPGP +S
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           PY IS  D  GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of HG10022635 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 261/334 (78.14%), Postives = 300/334 (89.82%), Query Frame = 0 

Query: 14  TRTQSVLKTLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSA 73
           +R    + T+R MSN+PE++VYGGP+ ++ NQRVTLT LRQK+++ EPITMVTAYDYPSA
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLEMAGIDIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH++ AGID+ LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAV++LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRSQGKNIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPF 253
           GGFR QGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAA T+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A+ GEVINKAL +YKEEVS   FPGP +S
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYTISDDDFSGFLKELQNMGLDEAASAAAAKANR 348
           PY IS  D  GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of HG10022635 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 525.4 bits (1352), Expect = 3.5e-149
Identity = 260/324 (80.25%), Postives = 297/324 (91.67%), Query Frame = 0 

Query: 22  TLRHMSNIPENSVYGGPQSRSPNQRVTLTNLRQKYKRAEPITMVTAYDYPSAVHLEMAGI 81
           ++R MSN+PEN+VYGGP+ ++PNQRVTLT+LRQK++R EPIT+VTAYDYPSAVHL+ AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVKILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRSQGK 201
           DTAV++LK+GGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFR QG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETALSLQEAGCFAVVLECVPPEVAAATTAALRIPTMGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAA T+AL+IPT+GIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFARTGEVINKALSDYKEEVSNGSFPGPPNSPYTISDDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A  GEVINKAL +YKEEVS   FPGP +SPY I+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 FSGFLKELQNMGLDEAASAAAAKA 346
             GFL ELQ +G D+AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

The following BLAST results are available for this feature:
BLAST of HG10022635 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038898896.16.8e-17188.763-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas... [more]
XP_004139651.11.5e-17088.763-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ... [more]
XP_008447278.14.1e-16888.18PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
KAG6575974.12.0e-16787.323-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu... [more]
XP_022953648.12.0e-16787.323-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
BLAST of HG10022635 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3152.2e-14878.143-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
O823574.9e-14880.253-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
Q9AWZ82.8e-13573.003-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
Q9AWZ74.6e-11465.953-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
B0TC104.5e-6949.073-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
BLAST of HG10022635 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K8B27.3e-17188.763-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BH282.0e-16888.183-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1GNW39.9e-16887.323-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JXR61.3e-16787.033-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1D0E37.8e-16585.343-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 ... [more]
BLAST of HG10022635 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G61530.11.6e-14978.14Phosphoenolpyruvate carboxylase family protein [more]
AT3G61530.21.6e-14978.14Phosphoenolpyruvate carboxylase family protein [more]
AT2G46110.13.5e-14980.25ketopantoate hydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 46..309
e-value: 8.8E-106
score: 353.0
IPR003700Ketopantoate hydroxymethyltransferaseTIGRFAMTIGR00222TIGR00222coord: 48..310
e-value: 2.3E-87
score: 290.8
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 15..347
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..315
score: 39.604656
IPR003700Ketopantoate hydroxymethyltransferaseCDDcd06557KPHMT-likecoord: 49..308
e-value: 4.45548E-146
score: 410.657
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 44..326
e-value: 2.1E-118
score: 396.5
NoneNo IPR availablePIRSRPIRSR000388-1PIRSR000388-1coord: 55..309
e-value: 5.6E-88
score: 292.3
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 15..347
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 46..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022635.1HG10022635.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0015940 pantothenate biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003824 catalytic activity