HG10022403 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022403
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Pectinesterase
Location	Chr05: 23927469 .. 23935019 (-)
RNA-Seq Expression	HG10022403
Synteny	HG10022403

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAGAGGTTCTTGTGTTTCCTATGTAATAATAATGGGTTTTTCCCTCTCATTACCATTCTTGGTTTTTTTCTCTTGGGTGTCGTCGGAGAGAGCTCCGGCGGCGGCGCTGGTGGTGGTGGTGGTGCAGGAGCAGAGGAATCATATGGTATGATCAGTTGGGATGATTTGCGTGTGGATGATTGGCGGCGGCAGGGGATCAGCTCCGGCGAGGGGTTGGAGAATGGGACTAGAATAATTGTGGTGGATAAGAACTGCTCCGGCGACTCCACGACGGTGCAAGGGGCGGGGGATAGGTTCCCTCATAATAACAAACAGAGAGTTAAGATTTATATTCTTCCTGGCATTTACAGGTTCGTTATATTTATATATATATTATCATTTTTTTTTTTTTTTTTAATTTTGAGTAATATTTGTGTTCTATTCTAAACGGCATCTATCTTTATACAATCCGGTCTAATCAAATAGTAAAAAAAAATATTTATTAAAAAAAAAAATTCATATAGCAAATTATGATTAAATCTTTTCCGCCTATTACGAGGAGTGTAGTAAATTTTAGTTAATTTTTTTTCTTTTTTTATTGGATTTTAGTACTCGTTATTATGCATATTAGAAATCACATAGGTAAATATCAAAATTAAAATTATTGTGTACATTATCACTTATCAGATCACCTTTAAAGATTAGGAAAAAATAATTTTTAAAAATTACCCATGACGAATTTTTAAAAAAACTACACTATCATCCTTCAATTTTTATTATATTTCAAAATTATTCTTGTGTAGAAACATTTTAGAATTTTTTGGCAAAAGTCAAAAACTTGAATAGGATAGTGTAGACCTAGAAACGAAAGTTTGGATCAATCACGTCCTCTTAGGCCACTTTCACAATGTTCCAAATTTTGATTTTTGTCTGAAACTCATATAAAAATTCTACTCCAATAATAGTTTTGAAATGATTATGAAAAGTCAATAACAATATTTTTTGTAACAAAAGCGTAAAGATTAAAATTATTAATATTGTTTTATTAGATGTCGACCTGAGAATTTTAATTATTAAAGAATATATCATTGTAGGTCGCTTTTTTTAAAGAACATTATAGGTTGCCTTTAAAGATTGACATATATTACAAATATTTTAATTAAATATTCATAGTGTTAATATTTATCAATAAATTTTTTTTAAAATATAATCATAATTAATTAAGATTCAATACCTTTTTTTTCATCATTATTAATATAAATTTTAAATTTTCATTTTATAATTGTTTTAAATTAATCAATAGACATTAATTTTAAAATCAAGCGTCCGTTTATAAACACTTAAAAAGTAAATCCAAACGGGTCTTAAAAATGAGTATTCAGTGGGAAAAAATGTTAAAAATATAAACCTTGAAACCTAGGGACCAATTTGAAACAAAAACTCAAAACTTAAAAGACTAAAAGTGCAACGTTTTAAAATTTTGGGACTAATTGAAATCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGATGAAGAGGTTCTTGTGTTTCCTATGTAATAATAATGGGTTTTTCCCTCTCATTACCATTCTTGGTTTTTTTCTCTTGGGTGTCGTCGGAGAGAGCTCCGGCGGCGGCGCTGGTGGTGGTGGTGGTGCAGGAGCAGAGGAATCATATGGTATGATCAGTTGGGATGATTTGCGTGTGGATGATTGGCGGCGGCAGGGGATCAGCTCCGGCGAGGGGTTGGAGAATGGGACTAGAATAATTGTGGTGGATAAGAACTGCTCCGGCGACTCCACGACGGTGCAAGGGGCGGGGGATAGGTTCCCTCATAATAACAAACAGAGAGTTAAGATTTATATTCTTCCTGGCATTTACAGGGCTTTAATTTCCCAAACTTTGTTAAGGTCGCCCACATCCATGCTAAGAGAGGGAAAATTCGGGATCCCCCAAGCGATTAAGGTCACGAAACTTCAGAAATCAGATTCTCCGTGA

Coding sequence (CDS)

ATGATGAAGAGGTTCTTGTGTTTCCTATGTAATAATAATGGGTTTTTCCCTCTCATTACCATTCTTGGTTTTTTTCTCTTGGGTGTCGTCGGAGAGAGCTCCGGCGGCGGCGCTGGTGGTGGTGGTGGTGCAGGAGCAGAGGAATCATATGGTATGATCAGTTGGGATGATTTGCGTGTGGATGATTGGCGGCGGCAGGGGATCAGCTCCGGCGAGGGGTTGGAGAATGGGACTAGAATAATTGTGGTGGATAAGAACTGCTCCGGCGACTCCACGACGGTGCAAGGGGCGGGGGATAGGTTCCCTCATAATAACAAACAGAGAGTTAAGATTTATATTCTTCCTGGCATTTACAGGGCTTTAATTTCCCAAACTTTGTTAAGGTCGCCCACATCCATGCTAAGAGAGGGAAAATTCGGGATCCCCCAAGCGATTAAGGTCACGAAACTTCAGAAATCAGATTCTCCGTGA

Protein sequence

MMKRFLCFLCNNNGFFPLITILGFFLLGVVGESSGGGAGGGGGAGAEESYGMISWDDLRVDDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYRALISQTLLRSPTSMLREGKFGIPQAIKVTKLQKSDSP

Homology

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BLAST of HG10022403 vs. NCBI nr
Match: XP_038896509.1 (pectinesterase QRT1 [Benincasa hispida])

HSP 1 Score: 181.0 bits (458), Expect = 7.8e-42
Identity = 99/121 (81.82%), Postives = 100/121 (82.64%), Query Frame = 0


Query: 2   MKRFLCFLCNNN-GFFPLITILGFFLLGVVGES--SGGGAGGGGGAGAEESYGMISWDDL 61
           MKRFLCFLCNNN GFFPLI IL F LL V GES  S GGAGGGGG   E SYGMISWDDL
Sbjct: 1   MKRFLCFLCNNNHGFFPLIFILVFLLLRVAGESSISNGGAGGGGGGVTERSYGMISWDDL 60

Query: 62  RVDDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIY 120
           RVD  RRQGISSG  L NGTRIIVVDKNCSG+STTVQGA D  PHNNKQRVKIYILPGIY
Sbjct: 61  RVDVSRRQGISSGNELGNGTRIIVVDKNCSGNSTTVQGAVDMAPHNNKQRVKIYILPGIY 120

[Close]

BLAST of HG10022403 vs. NCBI nr
Match: XP_008463039.1 (PREDICTED: pectinesterase QRT1 [Cucumis melo])

HSP 1 Score: 153.3 bits (386), Expect = 1.7e-33
Identity = 89/128 (69.53%), Postives = 94/128 (73.44%), Query Frame = 0


Query: 2   MKRFLCFLCNNN-------GFFP-LITILGFFLLGVV-GESSGGGAGGGGGAGAEESYGM 61
           M+ FLCFLCNNN       GFFP +I +L F LL VV GESSG G GG          G+
Sbjct: 1   MRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGG----------GV 60

Query: 62  ISWDDLRVDDWRRQGISSGEGL-ENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKI 120
           ISWDDLRVD W RQ +SSGEGL  NGTRIIVVDKNCSGDSTTVQGA D  PHNNKQRVKI
Sbjct: 61  ISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKI 118

[Close]

BLAST of HG10022403 vs. NCBI nr
Match: KAA0055635.1 (pectinesterase QRT1 [Cucumis melo var. makuwa] >TYK04405.1 pectinesterase QRT1 [Cucumis melo var. makuwa])

HSP 1 Score: 151.4 bits (381), Expect = 6.6e-33
Identity = 89/132 (67.42%), Postives = 96/132 (72.73%), Query Frame = 0


Query: 1   MMKRFLCFLCNNN-------GFFP-LITILGFFLLGVV-GESSGGGAGGGGGAGAEESYG 60
           MM+ FLCFLCNNN       GFFP +I +L F LL VV GESSG G GG          G
Sbjct: 2   MMRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGG----------G 61

Query: 61  MISWDDLRVDDWRRQGISSGEGL-ENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVK 120
           +ISWDDLRVD W RQ +SSGEGL  NGTRIIVVDKN SGDSTTVQGA D  PHNNKQRVK
Sbjct: 62  VISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNFSGDSTTVQGAVDMVPHNNKQRVK 121

Query: 121 IYILPGIYRALI 123
           IYILPGIYR ++
Sbjct: 122 IYILPGIYRFVL 123

[Close]

BLAST of HG10022403 vs. NCBI nr
Match: XP_004150299.1 (pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_015463 [Cucumis sativus])

HSP 1 Score: 150.6 bits (379), Expect = 1.1e-32
Identity = 79/109 (72.48%), Postives = 82/109 (75.23%), Query Frame = 0


Query: 11  NNNGFFPLITILGFFLLGVVGESSGGGAGGGGGAGAEESYGMISWDDLRVDDWRRQGISS 70
           NN GFFP I +LGF LL V G SSG G G           G+ISWDDLRVD WRRQ +SS
Sbjct: 2   NNGGFFPCIIVLGFLLLCVAGGSSGRGRG-----------GVISWDDLRVDVWRRQRMSS 61

Query: 71  GEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYR 120
            EGL NGTRIIVVDKNCSGDSTTVQGA D  PHNNKQRVKIYILPGIYR
Sbjct: 62  SEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYR 99

[Close]

BLAST of HG10022403 vs. NCBI nr
Match: XP_022975722.1 (pectinesterase QRT1 [Cucurbita maxima])

HSP 1 Score: 109.0 bits (271), Expect = 3.7e-20
Identity = 69/131 (52.67%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 17  PLITILGFFLLGVVGESSGGGAGGGG----GAGAEESYGMISWDDLRVDDWRRQGISS-- 76
           PL+ +L   L G+ G++SGGGA         AG  E Y  ISWDDLRVD   RQG+SS  
Sbjct: 5   PLLLLLLLLLFGLSGQASGGGAAAPAAAAFAAGGGERYA-ISWDDLRVDVPSRQGLSSGV 64

Query: 77  -GEGLEN--GTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYRALISQTLL 136
            G G++N  GTRIIVVD++ SGDSTTVQGA D  PHNN QRVKIYI PGIYR  +   + 
Sbjct: 65  HGGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVYIPIT 124

Query: 137 RSPTSMLREGK 139
           +   S++   K
Sbjct: 125 KPYISLIGNRK 134

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BLAST of HG10022403 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-10
Identity = 46/120 (38.33%), Postives = 58/120 (48.33%), Query Frame = 0


Query: 15  FFPLITILGFFLLGVVGESSGGGAGGGGGAGAEESYGMISWDDLRV-------------- 74
           F P + +L F ++  +  S     G        E    ISW+DLRV              
Sbjct: 6   FIPAVLLLCFGVMLCLKSSCALQIGNN-----NELKNYISWEDLRVVEDGRIERSFSIKE 65

Query: 75  -DDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYR 120
             +W     ++     N  R+IVVDKN  GDS TVQGA D  P +N QRVKI+ILPGIYR
Sbjct: 66  NSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYR 120

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BLAST of HG10022403 vs. ExPASy TrEMBL
Match: A0A0A0K706 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.0e-36
Identity = 86/121 (71.07%), Postives = 90/121 (74.38%), Query Frame = 0


Query: 2   MKRFLCFLC---NNNGFFPLITILGFFLLGVVGESSGGGAGGGGGAGAEESYGMISWDDL 61
           M+ FLCFLC   NN GFFP I +LGF LL V G SSG G G           G+ISWDDL
Sbjct: 1   MRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAGGSSGRGRG-----------GVISWDDL 60

Query: 62  RVDDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIY 120
           RVD WRRQ +SS EGL NGTRIIVVDKNCSGDSTTVQGA D  PHNNKQRVKIYILPGIY
Sbjct: 61  RVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIY 110

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BLAST of HG10022403 vs. ExPASy TrEMBL
Match: A0A1S3CIB1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 8.4e-34
Identity = 89/128 (69.53%), Postives = 94/128 (73.44%), Query Frame = 0


Query: 2   MKRFLCFLCNNN-------GFFP-LITILGFFLLGVV-GESSGGGAGGGGGAGAEESYGM 61
           M+ FLCFLCNNN       GFFP +I +L F LL VV GESSG G GG          G+
Sbjct: 1   MRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGG----------GV 60

Query: 62  ISWDDLRVDDWRRQGISSGEGL-ENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKI 120
           ISWDDLRVD W RQ +SSGEGL  NGTRIIVVDKNCSGDSTTVQGA D  PHNNKQRVKI
Sbjct: 61  ISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKI 118

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BLAST of HG10022403 vs. ExPASy TrEMBL
Match: A0A5D3BZ21 (Pectinesterase QRT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold54380G00010 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 3.2e-33
Identity = 89/132 (67.42%), Postives = 96/132 (72.73%), Query Frame = 0


Query: 1   MMKRFLCFLCNNN-------GFFP-LITILGFFLLGVV-GESSGGGAGGGGGAGAEESYG 60
           MM+ FLCFLCNNN       GFFP +I +L F LL VV GESSG G GG          G
Sbjct: 2   MMRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGG----------G 61

Query: 61  MISWDDLRVDDWRRQGISSGEGL-ENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVK 120
           +ISWDDLRVD W RQ +SSGEGL  NGTRIIVVDKN SGDSTTVQGA D  PHNNKQRVK
Sbjct: 62  VISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNFSGDSTTVQGAVDMVPHNNKQRVK 121

Query: 121 IYILPGIYRALI 123
           IYILPGIYR ++
Sbjct: 122 IYILPGIYRFVL 123

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BLAST of HG10022403 vs. ExPASy TrEMBL
Match: A0A6J1IDU4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.8e-20
Identity = 69/131 (52.67%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 17  PLITILGFFLLGVVGESSGGGAGGGG----GAGAEESYGMISWDDLRVDDWRRQGISS-- 76
           PL+ +L   L G+ G++SGGGA         AG  E Y  ISWDDLRVD   RQG+SS  
Sbjct: 5   PLLLLLLLLLFGLSGQASGGGAAAPAAAAFAAGGGERYA-ISWDDLRVDVPSRQGLSSGV 64

Query: 77  -GEGLEN--GTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYRALISQTLL 136
            G G++N  GTRIIVVD++ SGDSTTVQGA D  PHNN QRVKIYI PGIYR  +   + 
Sbjct: 65  HGGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVYIPIT 124

Query: 137 RSPTSMLREGK 139
           +   S++   K
Sbjct: 125 KPYISLIGNRK 134

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BLAST of HG10022403 vs. ExPASy TrEMBL
Match: A0A6J1CG92 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 8.4e-18
Identity = 55/84 (65.48%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 40  GGGGAGAEESYGMISWDDLRVDDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGD 99
           G  G G  E Y  I WDDL +D  RRQG++SG+    GTRIIVVDKNCSGDS TVQGA D
Sbjct: 23  GVAGGGRTERYA-IGWDDLHIDA-RRQGLNSGD--NGGTRIIVVDKNCSGDSPTVQGAVD 82

Query: 100 RFPHNNKQRVKIYILPGIYRALIS 124
             PH NKQRVKIYILPGIYR  ++
Sbjct: 83  LVPHYNKQRVKIYILPGIYREKVT 102

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BLAST of HG10022403 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 8.9e-12
Identity = 46/120 (38.33%), Postives = 58/120 (48.33%), Query Frame = 0


Query: 15  FFPLITILGFFLLGVVGESSGGGAGGGGGAGAEESYGMISWDDLRV-------------- 74
           F P + +L F ++  +  S     G        E    ISW+DLRV              
Sbjct: 6   FIPAVLLLCFGVMLCLKSSCALQIGNN-----NELKNYISWEDLRVVEDGRIERSFSIKE 65

Query: 75  -DDWRRQGISSGEGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYR 120
             +W     ++     N  R+IVVDKN  GDS TVQGA D  P +N QRVKI+ILPGIYR
Sbjct: 66  NSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYR 120

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BLAST of HG10022403 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 6.8e-04
Identity = 22/63 (34.92%), Postives = 33/63 (52.38%), Query Frame = 0


Query: 72  EGLENGTRIIVVDKNCSGDSTTVQGAGDRFPHNNKQRVKIYILPGIYRALISQTLLRSPT 131
           E  E   R+I+V++N  GD  T+  A    P  NK RV I + PGIY   ++  + R   
Sbjct: 58  EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 117

Query: 132 SML 135
           ++L
Sbjct: 118 TLL 120

The following BLAST results are available for this feature:

BLAST of HG10022403 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896509.1	7.8e-42	81.82	pectinesterase QRT1 [Benincasa hispida]	[more]
XP_008463039.1	1.7e-33	69.53	PREDICTED: pectinesterase QRT1 [Cucumis melo]	[more]
KAA0055635.1	6.6e-33	67.42	pectinesterase QRT1 [Cucumis melo var. makuwa] >TYK04405.1 pectinesterase QRT1 [...	[more]
XP_004150299.1	1.1e-32	72.48	pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_01546...	[more]
XP_022975722.1	3.7e-20	52.67	pectinesterase QRT1 [Cucurbita maxima]	[more]

BLAST of HG10022403 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FM79	1.3e-10	38.33	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]

BLAST of HG10022403 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K706	4.0e-36	71.07	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1	[more]
A0A1S3CIB1	8.4e-34	69.53	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1	[more]
A0A5D3BZ21	3.2e-33	67.42	Pectinesterase QRT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5438...	[more]
A0A6J1IDU4	1.8e-20	52.67	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1	[more]
A0A6J1CG92	8.4e-18	65.48	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1	[more]

BLAST of HG10022403 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55590.1	8.9e-12	38.33	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	6.8e-04	34.92	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 75..148 e-value: 6.8E-8 score: 34.2
None	No IPR available	PANTHER	PTHR31321:SF31	PECTINESTERASE QRT1	coord: 50..124
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 50..124
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 79..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022403.1	HG10022403.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds