HG10022316 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022316
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptiontranscription termination factor MTERF4, chloroplastic
LocationChr05: 23056826 .. 23058388 (+)
RNA-Seq ExpressionHG10022316
SyntenyHG10022316
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAGAGGCCATGCCGGTGTCGTAAAACCTAATTTTTCCTTCATGTTATTTGAGATGCCTACTCTTCTTGAATGTCAGAAGTTCAAATTTACTGCCACACCAACATTTCAAACGTCTTCTAACCATGAGAAAAGGAGAGGTTTAATTACACAGCTACAATATTCTCATGGTGATAGGAGAACAGTATTCAAATCTCTACATTCAACTGTTTCATCAAAGCCTTGCAATACTAAAGTGGGTAGAAAAGAAGGCAGTGCTTCATCACTATATAGTCGCCCTAGTTTAGTCGAGATGAAGAATGTGAGGCTTGAAAACCGTTCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTGTCGTTCCTGATGAACTTGATGGGCTGGAGCTTCCTGTAACAGTCGATGTTATGAAGGAACGTGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCTCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTACTTGATTACCTTGGTAAATTAGGCGTTAGGAAATCTACTTTCACTGAGTTCTTAAAAAGGTATCCGCAAGTCCTCCATTCCAGCGTTGTTGTCGACCTTGCTCCAGTCATCAAGTATCTTCAAGGCATGGATATTAAGCCAACTGATATCCCTCGCGTTCTCGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGTACTTCAGTGGCATACTTGGTAGGAATTGGAGTCGCAAGAAGGGAAATTGGAGGGGTTTTGACCAAATACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTTGAGTATCTTGAAAGTTTGGGCATTCCAAGATTAGCAGTGGCTAGGTTAATAGAGAAACGACCACACATTCTGGGGTTTGGATTAGAAAAAAAAGTCAAACCCAATATCCAATTGCTCTTGGAGTTCAATGTAAGAGAAGCATACCTTTCTTCTATAGTGGCACAGTATGCTGAGATCATAGGAATGGACCTGGAATCTAAGCTTCCAACTCAGAGAAATTTACTAAAATCATTAATTGAATTGAACGACGACAACTTTGGTGCTATCATCGAAAAGATGCCGCAAATTGTTAGCCTCAGCAGTTCAGCTGTGATAAACCATGTAGATTTATTAAAAACTTGTGGGTTTTCCTTGCTTCAAGTAAAGAATATGGTTGTAGGTTGCCCCCAATTGCTTGCTTTGAATATTGACATAATGAAACACAGCTTTGAGTTCTTCCAAATGGATATGGGAAGGCCTTTAGATGACTTAGTCACTTTTCCTGCATTCTTTACTTATGGTTTAGAGTCCACAATAAAGCCCAGACATAAGAAAGTTGTACAGAAGGGAATTAGTTGTTCACTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTTGCAGAAAGGATGAGCTATGATACCATAGATATGGAGGAGATGGAAACCACACCAACATTCGATATGAATTCTCTCATACAACCTCGTAACGATGAATCGGATTCTGAATACGAAGAGGATAGTTAG

mRNA sequence

ATGATCAGAGGCCATGCCGGTGTCGTAAAACCTAATTTTTCCTTCATGTTATTTGAGATGCCTACTCTTCTTGAATGTCAGAAGTTCAAATTTACTGCCACACCAACATTTCAAACGTCTTCTAACCATGAGAAAAGGAGAGGTTTAATTACACAGCTACAATATTCTCATGGTGATAGGAGAACAGTATTCAAATCTCTACATTCAACTGTTTCATCAAAGCCTTGCAATACTAAAGTGGGTAGAAAAGAAGGCAGTGCTTCATCACTATATAGTCGCCCTAGTTTAGTCGAGATGAAGAATGTGAGGCTTGAAAACCGTTCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTGTCGTTCCTGATGAACTTGATGGGCTGGAGCTTCCTGTAACAGTCGATGTTATGAAGGAACGTGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCTCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTACTTGATTACCTTGGTAAATTAGGCGTTAGGAAATCTACTTTCACTGAGTTCTTAAAAAGGTATCCGCAAGTCCTCCATTCCAGCGTTGTTGTCGACCTTGCTCCAGTCATCAAGTATCTTCAAGGCATGGATATTAAGCCAACTGATATCCCTCGCGTTCTCGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGTACTTCAGTGGCATACTTGGTAGGAATTGGAGTCGCAAGAAGGGAAATTGGAGGGGTTTTGACCAAATACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTTGAGTATCTTGAAAGTTTGGGCATTCCAAGATTAGCAGTGGCTAGGTTAATAGAGAAACGACCACACATTCTGGGGTTTGGATTAGAAAAAAAAGTCAAACCCAATATCCAATTGCTCTTGGAGTTCAATGTAAGAGAAGCATACCTTTCTTCTATAGTGGCACAGTATGCTGAGATCATAGGAATGGACCTGGAATCTAAGCTTCCAACTCAGAGAAATTTACTAAAATCATTAATTGAATTGAACGACGACAACTTTGGTGCTATCATCGAAAAGATGCCGCAAATTGTTAGCCTCAGCAGTTCAGCTGTGATAAACCATGTAGATTTATTAAAAACTTGTGGGTTTTCCTTGCTTCAAGTAAAGAATATGGTTGTAGGTTGCCCCCAATTGCTTGCTTTGAATATTGACATAATGAAACACAGCTTTGAGTTCTTCCAAATGGATATGGGAAGGCCTTTAGATGACTTAGTCACTTTTCCTGCATTCTTTACTTATGGTTTAGAGTCCACAATAAAGCCCAGACATAAGAAAGTTGTACAGAAGGGAATTAGTTGTTCACTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTTGCAGAAAGGATGAGCTATGATACCATAGATATGGAGGAGATGGAAACCACACCAACATTCGATATGAATTCTCTCATACAACCTCGTAACGATGAATCGGATTCTGAATACGAAGAGGATAGTTAG

Coding sequence (CDS)

ATGATCAGAGGCCATGCCGGTGTCGTAAAACCTAATTTTTCCTTCATGTTATTTGAGATGCCTACTCTTCTTGAATGTCAGAAGTTCAAATTTACTGCCACACCAACATTTCAAACGTCTTCTAACCATGAGAAAAGGAGAGGTTTAATTACACAGCTACAATATTCTCATGGTGATAGGAGAACAGTATTCAAATCTCTACATTCAACTGTTTCATCAAAGCCTTGCAATACTAAAGTGGGTAGAAAAGAAGGCAGTGCTTCATCACTATATAGTCGCCCTAGTTTAGTCGAGATGAAGAATGTGAGGCTTGAAAACCGTTCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTGTCGTTCCTGATGAACTTGATGGGCTGGAGCTTCCTGTAACAGTCGATGTTATGAAGGAACGTGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCTCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTACTTGATTACCTTGGTAAATTAGGCGTTAGGAAATCTACTTTCACTGAGTTCTTAAAAAGGTATCCGCAAGTCCTCCATTCCAGCGTTGTTGTCGACCTTGCTCCAGTCATCAAGTATCTTCAAGGCATGGATATTAAGCCAACTGATATCCCTCGCGTTCTCGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGTACTTCAGTGGCATACTTGGTAGGAATTGGAGTCGCAAGAAGGGAAATTGGAGGGGTTTTGACCAAATACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTTGAGTATCTTGAAAGTTTGGGCATTCCAAGATTAGCAGTGGCTAGGTTAATAGAGAAACGACCACACATTCTGGGGTTTGGATTAGAAAAAAAAGTCAAACCCAATATCCAATTGCTCTTGGAGTTCAATGTAAGAGAAGCATACCTTTCTTCTATAGTGGCACAGTATGCTGAGATCATAGGAATGGACCTGGAATCTAAGCTTCCAACTCAGAGAAATTTACTAAAATCATTAATTGAATTGAACGACGACAACTTTGGTGCTATCATCGAAAAGATGCCGCAAATTGTTAGCCTCAGCAGTTCAGCTGTGATAAACCATGTAGATTTATTAAAAACTTGTGGGTTTTCCTTGCTTCAAGTAAAGAATATGGTTGTAGGTTGCCCCCAATTGCTTGCTTTGAATATTGACATAATGAAACACAGCTTTGAGTTCTTCCAAATGGATATGGGAAGGCCTTTAGATGACTTAGTCACTTTTCCTGCATTCTTTACTTATGGTTTAGAGTCCACAATAAAGCCCAGACATAAGAAAGTTGTACAGAAGGGAATTAGTTGTTCACTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTTGCAGAAAGGATGAGCTATGATACCATAGATATGGAGGAGATGGAAACCACACCAACATTCGATATGAATTCTCTCATACAACCTCGTAACGATGAATCGGATTCTGAATACGAAGAGGATAGTTAG

Protein sequence

MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDRRTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS
Homology
BLAST of HG10022316 vs. NCBI nr
Match: XP_038890870.1 (transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038890871.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida])

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-273
Identity = 485/520 (93.27%), Postives = 500/520 (96.15%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGV+KP+FSFM+FEMPT LEC KFK TA  TFQ  SN+EKRRGLIT+LQYSHGDR
Sbjct: 1   MIRGHAGVLKPSFSFMIFEMPT-LECHKFKITAPSTFQMPSNYEKRRGLITKLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120
           RTVFKSLHST+SSKP N +VGRKE  ASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV
Sbjct: 61  RTVFKSLHSTISSKPYNAQVGRKEDKASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180

Query: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTM 240
           RKSTFTEFLKRYPQVLHSSVVVDLAPV+KYLQGMDIKP DIPR+LERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRILERYPEVLGFKLEGTM 240

Query: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIE 360
           PHILGFGLEK VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLE KL TQRNLLKSLIE
Sbjct: 301 PHILGFGLEKNVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLEGKLQTQRNLLKSLIE 360

Query: 361 LNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMK 420
           L+DDNFGAIIEKMPQIVSLSSSAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIMK
Sbjct: 361 LDDDNFGAIIEKMPQIVSLSSSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIMK 420

Query: 421 HSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFA 480
           HSFEFF MDMGRPL+DLV FPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNCSDEKF 
Sbjct: 421 HSFEFFHMDMGRPLEDLVAFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCSDEKFV 480

Query: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
           ERMSYDTIDMEEMET PTFDMNSLIQP+N+ESDSEYEEDS
Sbjct: 481 ERMSYDTIDMEEMETIPTFDMNSLIQPQNNESDSEYEEDS 519

BLAST of HG10022316 vs. NCBI nr
Match: XP_008465763.1 (PREDICTED: transcription termination factor MTERF4, chloroplastic [Cucumis melo] >XP_008465764.1 PREDICTED: transcription termination factor MTERF4, chloroplastic [Cucumis melo] >KAA0066609.1 transcription termination factor MTERF4 [Cucumis melo var. makuwa] >TYK14207.1 transcription termination factor MTERF4 [Cucumis melo var. makuwa])

HSP 1 Score: 941.4 bits (2432), Expect = 3.3e-270
Identity = 482/521 (92.51%), Postives = 497/521 (95.39%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGVVKPN SFMLFEM T LEC  FKFTATPTF TS  HEKRR L TQLQYSHGDR
Sbjct: 1   MIRGHAGVVKPNVSFMLFEMST-LECHNFKFTATPTFLTSCIHEKRRCLTTQLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSA-SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120
           RTVFKSLHST+SSKP ++KVGRK GS+ SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV
Sbjct: 61  RTVFKSLHSTISSKPYSSKVGRKGGSSGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120

Query: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180
           VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG
Sbjct: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180

Query: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGT 240
           VRKSTFTEFLKRYPQVLHSSVVVDLAPV+KYLQGMDIKP DIPRVLERYPEVLGFKLEGT
Sbjct: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRVLERYPEVLGFKLEGT 240

Query: 241 MSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEK 300
           MSTSVAYLVGIGVARREIGG+LTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEK
Sbjct: 241 MSTSVAYLVGIGVARREIGGILTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEK 300

Query: 301 RPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLI 360
           RPHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIGMDLE+ L TQRNLLKSLI
Sbjct: 301 RPHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGMDLEANLQTQRNLLKSLI 360

Query: 361 ELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIM 420
           EL++DNFG IIEKMPQIVSLS SAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIM
Sbjct: 361 ELDNDNFGTIIEKMPQIVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIM 420

Query: 421 KHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKF 480
           KHSFEFFQM+MGRPL+DL TFPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNC+DEKF
Sbjct: 421 KHSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKF 480

Query: 481 AERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
            ERMSYDTIDMEEMETTPTFDMNSL+QPRNDESDSEYEEDS
Sbjct: 481 VERMSYDTIDMEEMETTPTFDMNSLVQPRNDESDSEYEEDS 520

BLAST of HG10022316 vs. NCBI nr
Match: XP_004143818.1 (transcription termination factor MTERF4, chloroplastic [Cucumis sativus] >KGN51142.1 hypothetical protein Csa_008517 [Cucumis sativus])

HSP 1 Score: 936.0 bits (2418), Expect = 1.4e-268
Identity = 479/521 (91.94%), Postives = 494/521 (94.82%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGV KPNFS MLFEM T LEC  FKFTAT TFQ   NHEKR+GLITQLQYSHGDR
Sbjct: 1   MIRGHAGVAKPNFSLMLFEMST-LECHNFKFTATRTFQMPYNHEKRKGLITQLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGS-ASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120
           RTVFKSLHST+SSK  ++KV RKEGS  SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV
Sbjct: 61  RTVFKSLHSTISSKSYSSKVDRKEGSIGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120

Query: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180
           VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPL+LGCSVKKNMIPVLDYLGKLG
Sbjct: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLILGCSVKKNMIPVLDYLGKLG 180

Query: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGT 240
           VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKP DIPRVLERYPEVLGFKLEGT
Sbjct: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPNDIPRVLERYPEVLGFKLEGT 240

Query: 241 MSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEK 300
           MSTSVAYLVGIGV RREIGGVLTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEK
Sbjct: 241 MSTSVAYLVGIGVTRREIGGVLTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEK 300

Query: 301 RPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLI 360
           RPHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIG+DLE+ L TQRNLLKSLI
Sbjct: 301 RPHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGIDLEANLQTQRNLLKSLI 360

Query: 361 ELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIM 420
           EL++DNFG IIEKMPQ+VSLS SAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIM
Sbjct: 361 ELDNDNFGTIIEKMPQVVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIM 420

Query: 421 KHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKF 480
           KHSFEFFQM+MGRPL+DL TFPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNC+DEKF
Sbjct: 421 KHSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKF 480

Query: 481 AERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
            ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS
Sbjct: 481 MERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 520

BLAST of HG10022316 vs. NCBI nr
Match: XP_023538106.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023538107.1 transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 926.8 bits (2394), Expect = 8.3e-266
Identity = 466/520 (89.62%), Postives = 497/520 (95.58%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGH GVVKPNFS ML EMPT L+CQKFKF  T TFQTS NHEKRRGLIT+LQYS GDR
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPT-LKCQKFKFITTSTFQTSPNHEKRRGLITKLQYSRGDR 61

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120
           RTV KSLHST++SKP ++KV RKEGSASSLYSRPSLVEMKNVR ENR+KVYEFLRGIG+V
Sbjct: 62  RTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 121

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGV
Sbjct: 122 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 181

Query: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLHSSVVVDL PV+KYLQGMDIKP+DIPRVLERYPEVLGFKLEGTM
Sbjct: 182 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 241

Query: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 242 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 301

Query: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIE 360
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLE+KLP+QRNLLKS+IE
Sbjct: 302 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 361

Query: 361 LNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMK 420
           L++DNFG IIEKMPQ+VSLSSSAV +HVD LK+CGFSL QVKNMV+GCPQLLALN+DIMK
Sbjct: 362 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 421

Query: 421 HSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFA 480
           HSF+FFQ +MGRPLDDLV FPAFFTYGLESTIKPRH+KVVQKG++CSLSWLLNCSDEKFA
Sbjct: 422 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 481

Query: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
           ERM+YDTIDMEEMET PTFDM+SL QPR DESDS+YEEDS
Sbjct: 482 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDS 520

BLAST of HG10022316 vs. NCBI nr
Match: XP_022938209.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938210.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938211.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 923.7 bits (2386), Expect = 7.0e-265
Identity = 465/520 (89.42%), Postives = 496/520 (95.38%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGH GVVKPNFS ML EMPT L+C KFKF  T TFQTS NHEKRRGLIT+LQYS GD 
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPT-LKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDW 61

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120
           RTV KSLHST++SKP ++KVGRKEGSASSLYSRPSLVEMKNVR ENR+KVYEFLRGIG+V
Sbjct: 62  RTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 121

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGV
Sbjct: 122 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 181

Query: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLHSSVVVDL PV+KYLQGMDIKP+DIPRVLERYPEVLGFKLEGTM
Sbjct: 182 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 241

Query: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 242 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 301

Query: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIE 360
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLE+KLP+QRNLLKS+IE
Sbjct: 302 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 361

Query: 361 LNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMK 420
           L++DNFG IIEKMPQ+VSLSSSAV +HVD LK+CGFSL QVKNMV+GCPQLLALN+DIMK
Sbjct: 362 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 421

Query: 421 HSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFA 480
           HSF+FFQ +MGRPLDDLV FPAFFTYGLESTIKPRH+KVVQKG++CSLSWLLNCSDEKFA
Sbjct: 422 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 481

Query: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
           ERM+YDTIDMEEMET PTFDM+SL QPR DESDS+YEEDS
Sbjct: 482 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDS 520

BLAST of HG10022316 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 3.8e-197
Identity = 358/531 (67.42%), Postives = 425/531 (80.04%), Query Frame = 0

Query: 2   IRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKR--RGLITQLQYSHGD 61
           IR   G  KP F  + FE P+    +  +  +  T+    NH+KR   G+   +Q +  +
Sbjct: 3   IRFCNGFTKPGFLLVHFEPPSFFAVRS-RSLSDSTYGNLCNHKKRPGTGIGLTVQCAIAN 62

Query: 62  RRTVFKSLHS--TVSSKPCNTKVGR-------KEGSASSLYSRPSLVEMKNVRLENRSKV 121
           RR   +SL S     S   ++  GR       K   + SLYSRPSL++M   +  NR+KV
Sbjct: 63  RRFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAKV 122

Query: 122 YEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIP 181
           YEFLRGIG+VPDELDGLELPVT DVMKERV+FL KLGLTIEDINNYPLVLGCSVKKNM+P
Sbjct: 123 YEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMVP 182

Query: 182 VLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPE 241
           VLDYLGKLGVRKSTFTEFL+RYPQVLHSSVV+DLAPV+KYLQG+DIKP+D+PRVLERYPE
Sbjct: 183 VLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYPE 242

Query: 242 VLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPR 301
           VLGFKLEGTMSTSVAYLVGIGVARREIGG+LT+YPEILGMRVAR+IKP VEYLE LGIPR
Sbjct: 243 VLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIPR 302

Query: 302 LAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPT 361
           LA ARLIEKRPHILGF L+  VKPN+Q+L +FNVRE  L SI+AQY EIIG+DL+ KL T
Sbjct: 303 LAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLDT 362

Query: 362 QRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQ 421
           QR LL S I LN ++ G++IE+MPQ VSLS S ++ H+D L  CGFS+ Q + MV+GCPQ
Sbjct: 363 QRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCPQ 422

Query: 422 LLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSW 481
           +LALN+ IMK SFE+FQ +M RPL DLV FPAFFTYGLEST+KPRHKK+++KGI CSL+W
Sbjct: 423 VLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLAW 482

Query: 482 LLNCSDEKFAERMSYDTIDMEEMETTP-TFDMNSLIQP-RNDESDSEYEED 520
           +LNCSDEKF +RMSYDTID+EE+ET P +FDMN+L+QP R +ESDSEYEE+
Sbjct: 483 MLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEE 532

BLAST of HG10022316 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.7e-160
Identity = 277/435 (63.68%), Postives = 354/435 (81.38%), Query Frame = 0

Query: 87  ASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKL 146
           +SSLY+RPSL++M+  R   R+ V  FL  +GV P EL GLELP TVDVM+ERV+FL  L
Sbjct: 63  SSSLYARPSLLDMERGRAARRADVDAFLASLGVDPGELAGLELPATVDVMRERVEFLHSL 122

Query: 147 GLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAP 206
            L+ ED+  YPL LGCSV+KNM+PVLDYLGKLGVR+    + L+RYPQVLH+SVVVDLAP
Sbjct: 123 DLSNEDLAAYPLALGCSVRKNMVPVLDYLGKLGVRQDALPDLLRRYPQVLHASVVVDLAP 182

Query: 207 VIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPE 266
           V+KYLQGMD++P D+PRVLERYPE+LGFKLEGTMSTS+AYLVGIGVARR++G V+T++PE
Sbjct: 183 VVKYLQGMDVRPHDVPRVLERYPELLGFKLEGTMSTSIAYLVGIGVARRQVGSVITRFPE 242

Query: 267 ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVRE 326
           +LGMRV ++IKPFVE+LE +G+ RLA+AR+IEK+P++LGFGLE KVKPNI+ LLEF VR+
Sbjct: 243 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLEDKVKPNIEALLEFGVRK 302

Query: 327 AYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN 386
             L+ IVAQY +I+G++L  KL TQ++L +S I ++ ++FG +IE+MPQ +SL  +AV+ 
Sbjct: 303 EALAFIVAQYPDILGIELRDKLATQQSLFESSILVSSEDFGRVIERMPQAISLGRTAVLK 362

Query: 387 HVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTY 446
           HV+ L +CGF L QV  MVV CPQLLALN+DIMK SFE+FQ +M R L++LV FPAFFTY
Sbjct: 363 HVNFLTSCGFLLSQVSKMVVACPQLLALNMDIMKMSFEYFQNEMERDLEELVEFPAFFTY 422

Query: 447 GLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTIDMEEMETTPTFDMNSLIQ 506
           GLEST++PRH+ V +KG +CSL+WLLNCSD KF ERM YDTI +EEME   +FD N+L +
Sbjct: 423 GLESTVRPRHEMVAKKGFTCSLAWLLNCSDAKFDERMKYDTIGIEEMEVDNSFDTNTLSE 482

Query: 507 PRNDE-SDSEYEEDS 521
              DE  D + +EDS
Sbjct: 483 RVEDEVEDEDLDEDS 497

BLAST of HG10022316 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 6.0e-158
Identity = 270/434 (62.21%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 87  ASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKL 146
           +SSLY+RPSL++M+  R   R+ V  FL  +GV P EL GLELPVTVDVM+ER +FL  L
Sbjct: 54  SSSLYARPSLLDMERDRATRRADVDAFLTSLGVDPGELAGLELPVTVDVMRERAEFLGSL 113

Query: 147 GLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAP 206
           GLT ED+  YPL LGCSV+KNM+PVLDYLGKLGVR+    + L+RYPQVLH+SVVVDLAP
Sbjct: 114 GLTQEDLAAYPLALGCSVRKNMVPVLDYLGKLGVRRDALPDLLRRYPQVLHASVVVDLAP 173

Query: 207 VIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPE 266
           V+KYLQGMD++PTD+PRVLERYPE+LGFKLEGTMSTSVAYLVGIGV RR++G V+T++PE
Sbjct: 174 VVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPE 233

Query: 267 ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVRE 326
           +LGMRV ++IKPFVE+LE +G+ RLA+AR+IEK+P++LGFGL++KVKPNI+ L++  VR+
Sbjct: 234 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRK 293

Query: 327 AYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN 386
             L+SIV QY +++G++L  KL  Q++L +S I ++ ++FG ++E+MPQ +SL  +AV+ 
Sbjct: 294 EALASIVMQYPDVLGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAVLK 353

Query: 387 HVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTY 446
           HV+ L  CGF L QV  MVV CPQLLALNIDIM+ +FE+F+ +M R L++LV FPAFFTY
Sbjct: 354 HVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFFTY 413

Query: 447 GLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTIDMEEMETTPTFDMNSLIQ 506
           G+EST++PRH+ V +KG++CSL+WLLNCSD KF ERM YDTI +EEME   + DMN+ + 
Sbjct: 414 GIESTVRPRHEMVSRKGLTCSLAWLLNCSDAKFDERMKYDTIGVEEMEREDSSDMNASV- 473

Query: 507 PRNDESDSEYEEDS 521
              DE +SE  EDS
Sbjct: 474 ---DEVESEEYEDS 483

BLAST of HG10022316 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 2.3e-32
Identity = 91/363 (25.07%), Postives = 173/363 (47.66%), Query Frame = 0

Query: 165 KKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRV 224
           ++ +  +++YL   GVR+      + R P++L  S + ++   + +   M +   D   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 225 LERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLE 284
           +  YP+++GF     M   + YL   G++  E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 285 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDL 344
            LGIP+  + R++  +P +    LEK + P ++ L E  +    + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 345 ESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN-HVDLLKTCGFSLLQVKN 404
             K+      L +   +   + G +I   P ++  S    +  ++    + G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 405 MVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKG 464
           M+   P LL  N+D ++  + + +  M RPL DL+ FP FF+Y LE  I PRH  +V+  
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 465 ISCSLSWLLNCSDEKFAERMSYDTIDMEE-------METTPTFDMNSLIQPRNDESDSEY 520
           ++  L ++L C+DE+F ER   D ++  E        E +   D N   Q      ++E 
Sbjct: 578 VNFKLRYMLACTDEEF-ERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEE 637

BLAST of HG10022316 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 69/276 (25.00%), Postives = 146/276 (52.90%), Query Frame = 0

Query: 216 IKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 275
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 276 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 335
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 336 QYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN-HVDLLKT 395
           +   ++G  ++ +L      LKS + L++D   +++   PQ++    + ++  + D LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 396 CGFSLLQVKNMVVGCPQLLALNI-DIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTI 455
           CGF   Q+  MV G PQ+L  ++ + ++    F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 456 KPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTI 489
           + R K V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of HG10022316 vs. ExPASy TrEMBL
Match: A0A1S3CR33 (transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503367 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.6e-270
Identity = 482/521 (92.51%), Postives = 497/521 (95.39%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGVVKPN SFMLFEM T LEC  FKFTATPTF TS  HEKRR L TQLQYSHGDR
Sbjct: 1   MIRGHAGVVKPNVSFMLFEMST-LECHNFKFTATPTFLTSCIHEKRRCLTTQLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSA-SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120
           RTVFKSLHST+SSKP ++KVGRK GS+ SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV
Sbjct: 61  RTVFKSLHSTISSKPYSSKVGRKGGSSGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120

Query: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180
           VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG
Sbjct: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180

Query: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGT 240
           VRKSTFTEFLKRYPQVLHSSVVVDLAPV+KYLQGMDIKP DIPRVLERYPEVLGFKLEGT
Sbjct: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRVLERYPEVLGFKLEGT 240

Query: 241 MSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEK 300
           MSTSVAYLVGIGVARREIGG+LTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEK
Sbjct: 241 MSTSVAYLVGIGVARREIGGILTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEK 300

Query: 301 RPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLI 360
           RPHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIGMDLE+ L TQRNLLKSLI
Sbjct: 301 RPHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGMDLEANLQTQRNLLKSLI 360

Query: 361 ELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIM 420
           EL++DNFG IIEKMPQIVSLS SAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIM
Sbjct: 361 ELDNDNFGTIIEKMPQIVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIM 420

Query: 421 KHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKF 480
           KHSFEFFQM+MGRPL+DL TFPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNC+DEKF
Sbjct: 421 KHSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKF 480

Query: 481 AERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
            ERMSYDTIDMEEMETTPTFDMNSL+QPRNDESDSEYEEDS
Sbjct: 481 VERMSYDTIDMEEMETTPTFDMNSLVQPRNDESDSEYEEDS 520

BLAST of HG10022316 vs. ExPASy TrEMBL
Match: A0A5A7VMZ0 (Transcription termination factor MTERF4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5G00320 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.6e-270
Identity = 482/521 (92.51%), Postives = 497/521 (95.39%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGVVKPN SFMLFEM T LEC  FKFTATPTF TS  HEKRR L TQLQYSHGDR
Sbjct: 1   MIRGHAGVVKPNVSFMLFEMST-LECHNFKFTATPTFLTSCIHEKRRCLTTQLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSA-SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120
           RTVFKSLHST+SSKP ++KVGRK GS+ SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV
Sbjct: 61  RTVFKSLHSTISSKPYSSKVGRKGGSSGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120

Query: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180
           VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG
Sbjct: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180

Query: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGT 240
           VRKSTFTEFLKRYPQVLHSSVVVDLAPV+KYLQGMDIKP DIPRVLERYPEVLGFKLEGT
Sbjct: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRVLERYPEVLGFKLEGT 240

Query: 241 MSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEK 300
           MSTSVAYLVGIGVARREIGG+LTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEK
Sbjct: 241 MSTSVAYLVGIGVARREIGGILTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEK 300

Query: 301 RPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLI 360
           RPHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIGMDLE+ L TQRNLLKSLI
Sbjct: 301 RPHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGMDLEANLQTQRNLLKSLI 360

Query: 361 ELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIM 420
           EL++DNFG IIEKMPQIVSLS SAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIM
Sbjct: 361 ELDNDNFGTIIEKMPQIVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIM 420

Query: 421 KHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKF 480
           KHSFEFFQM+MGRPL+DL TFPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNC+DEKF
Sbjct: 421 KHSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKF 480

Query: 481 AERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
            ERMSYDTIDMEEMETTPTFDMNSL+QPRNDESDSEYEEDS
Sbjct: 481 VERMSYDTIDMEEMETTPTFDMNSLVQPRNDESDSEYEEDS 520

BLAST of HG10022316 vs. ExPASy TrEMBL
Match: A0A0A0KRV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.6e-269
Identity = 479/521 (91.94%), Postives = 494/521 (94.82%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGHAGV KPNFS MLFEM T LEC  FKFTAT TFQ   NHEKR+GLITQLQYSHGDR
Sbjct: 1   MIRGHAGVAKPNFSLMLFEMST-LECHNFKFTATRTFQMPYNHEKRKGLITQLQYSHGDR 60

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGS-ASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120
           RTVFKSLHST+SSK  ++KV RKEGS  SSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV
Sbjct: 61  RTVFKSLHSTISSKSYSSKVDRKEGSIGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGV 120

Query: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLG 180
           VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPL+LGCSVKKNMIPVLDYLGKLG
Sbjct: 121 VPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLILGCSVKKNMIPVLDYLGKLG 180

Query: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGT 240
           VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKP DIPRVLERYPEVLGFKLEGT
Sbjct: 181 VRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPNDIPRVLERYPEVLGFKLEGT 240

Query: 241 MSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEK 300
           MSTSVAYLVGIGV RREIGGVLTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEK
Sbjct: 241 MSTSVAYLVGIGVTRREIGGVLTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEK 300

Query: 301 RPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLI 360
           RPHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIG+DLE+ L TQRNLLKSLI
Sbjct: 301 RPHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGIDLEANLQTQRNLLKSLI 360

Query: 361 ELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIM 420
           EL++DNFG IIEKMPQ+VSLS SAVINHVD LKTCGFSLLQVKNMV+GCPQLLALNIDIM
Sbjct: 361 ELDNDNFGTIIEKMPQVVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIM 420

Query: 421 KHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKF 480
           KHSFEFFQM+MGRPL+DL TFPAFFTYGLESTIKPRH+KVVQKGISCSLSWLLNC+DEKF
Sbjct: 421 KHSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKF 480

Query: 481 AERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
            ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS
Sbjct: 481 MERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 520

BLAST of HG10022316 vs. ExPASy TrEMBL
Match: A0A6J1FDE7 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444359 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.4e-265
Identity = 465/520 (89.42%), Postives = 496/520 (95.38%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIRGH GVVKPNFS ML EMPT L+C KFKF  T TFQTS NHEKRRGLIT+LQYS GD 
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPT-LKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDW 61

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120
           RTV KSLHST++SKP ++KVGRKEGSASSLYSRPSLVEMKNVR ENR+KVYEFLRGIG+V
Sbjct: 62  RTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 121

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGV
Sbjct: 122 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 181

Query: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLHSSVVVDL PV+KYLQGMDIKP+DIPRVLERYPEVLGFKLEGTM
Sbjct: 182 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 241

Query: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 242 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 301

Query: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIE 360
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLE+KLP+QRNLLKS+IE
Sbjct: 302 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 361

Query: 361 LNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMK 420
           L++DNFG IIEKMPQ+VSLSSSAV +HVD LK+CGFSL QVKNMV+GCPQLLALN+DIMK
Sbjct: 362 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 421

Query: 421 HSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFA 480
           HSF+FFQ +MGRPLDDLV FPAFFTYGLESTIKPRH+KVVQKG++CSLSWLLNCSDEKFA
Sbjct: 422 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 481

Query: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
           ERM+YDTIDMEEMET PTFDM+SL QPR DESDS+YEEDS
Sbjct: 482 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDS 520

BLAST of HG10022316 vs. ExPASy TrEMBL
Match: A0A6J1DUY1 (transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023323 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.5e-262
Identity = 461/520 (88.65%), Postives = 496/520 (95.38%), Query Frame = 0

Query: 1   MIRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKRRGLITQLQYSHGDR 60
           MIR HAGVVKPNFS ++ +MPT L+C KF FT T  FQT SN ++RRGLIT+LQYS GDR
Sbjct: 2   MIRRHAGVVKPNFSIIVPKMPT-LKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDR 61

Query: 61  RTVFKSLHSTVSSKPCNTKVGRKEGSASSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120
           RTV KSLHST++SKP +T+VGRKEGSASSLYS PSLVEMKNVRLENR+KVYEFL GIG+V
Sbjct: 62  RTVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIV 121

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV
Sbjct: 122 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 181

Query: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLH+SVVVDLAPV+KYLQGMDIKP DIP VLERYPEVLGFKLEGTM
Sbjct: 182 RKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTM 241

Query: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 242 STSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 301

Query: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPTQRNLLKSLIE 360
           PHILGFGLEKKVKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLE+KL +QRNLLKS+IE
Sbjct: 302 PHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIE 361

Query: 361 LNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQLLALNIDIMK 420
           L+DDNFG II KMPQIVSLSSSAVINHVD LK+CGFSL+QVKNMV+GCPQLLALN+DIMK
Sbjct: 362 LDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMK 421

Query: 421 HSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSWLLNCSDEKFA 480
           HSF+FFQM+MGRPLDDLVTFPAFFTYGLESTIKPRH+KVVQKGI+CSLSWLLNCSDEKFA
Sbjct: 422 HSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFA 481

Query: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDS 521
           ERM+YDTIDMEEMET PTFDMNSLIQPRND+SDS+YEEDS
Sbjct: 482 ERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDS 520

BLAST of HG10022316 vs. TAIR 10
Match: AT4G02990.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 689.1 bits (1777), Expect = 2.7e-198
Identity = 358/531 (67.42%), Postives = 425/531 (80.04%), Query Frame = 0

Query: 2   IRGHAGVVKPNFSFMLFEMPTLLECQKFKFTATPTFQTSSNHEKR--RGLITQLQYSHGD 61
           IR   G  KP F  + FE P+    +  +  +  T+    NH+KR   G+   +Q +  +
Sbjct: 3   IRFCNGFTKPGFLLVHFEPPSFFAVRS-RSLSDSTYGNLCNHKKRPGTGIGLTVQCAIAN 62

Query: 62  RRTVFKSLHS--TVSSKPCNTKVGR-------KEGSASSLYSRPSLVEMKNVRLENRSKV 121
           RR   +SL S     S   ++  GR       K   + SLYSRPSL++M   +  NR+KV
Sbjct: 63  RRFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAKV 122

Query: 122 YEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIP 181
           YEFLRGIG+VPDELDGLELPVT DVMKERV+FL KLGLTIEDINNYPLVLGCSVKKNM+P
Sbjct: 123 YEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMVP 182

Query: 182 VLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRVLERYPE 241
           VLDYLGKLGVRKSTFTEFL+RYPQVLHSSVV+DLAPV+KYLQG+DIKP+D+PRVLERYPE
Sbjct: 183 VLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYPE 242

Query: 242 VLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPR 301
           VLGFKLEGTMSTSVAYLVGIGVARREIGG+LT+YPEILGMRVAR+IKP VEYLE LGIPR
Sbjct: 243 VLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIPR 302

Query: 302 LAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLESKLPT 361
           LA ARLIEKRPHILGF L+  VKPN+Q+L +FNVRE  L SI+AQY EIIG+DL+ KL T
Sbjct: 303 LAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLDT 362

Query: 362 QRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCGFSLLQVKNMVVGCPQ 421
           QR LL S I LN ++ G++IE+MPQ VSLS S ++ H+D L  CGFS+ Q + MV+GCPQ
Sbjct: 363 QRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCPQ 422

Query: 422 LLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKGISCSLSW 481
           +LALN+ IMK SFE+FQ +M RPL DLV FPAFFTYGLEST+KPRHKK+++KGI CSL+W
Sbjct: 423 VLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLAW 482

Query: 482 LLNCSDEKFAERMSYDTIDMEEMETTP-TFDMNSLIQP-RNDESDSEYEED 520
           +LNCSDEKF +RMSYDTID+EE+ET P +FDMN+L+QP R +ESDSEYEE+
Sbjct: 483 MLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEE 532

BLAST of HG10022316 vs. TAIR 10
Match: AT2G44020.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 209/424 (49.29%), Postives = 315/424 (74.29%), Query Frame = 0

Query: 96  LVEMKNVRLENRSKVYEFLRGIGVVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINN 155
           +++ K  +L NR K+ ++L+G+G++ DEL+ +ELP T++VM ERV+FL KLGLTI+DIN 
Sbjct: 72  VIQGKKEKLVNRVKICDYLKGLGIITDELESIELPSTIEVMCERVEFLQKLGLTIDDINE 131

Query: 156 YPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMD 215
           YPL+LGCSV+KN+IPVL YL K+G+ +S   EF+K YPQVLH+SVVV+LAPV+K+L+G+D
Sbjct: 132 YPLMLGCSVRKNLIPVLAYLEKIGISRSKLGEFVKNYPQVLHASVVVELAPVVKFLRGLD 191

Query: 216 IKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 275
           ++  D+  VL +YPE+LGFKLEGTMSTSVAYLV IGV+ R+IG ++T+YP +LGMRV  +
Sbjct: 192 VEKQDLGYVLMKYPELLGFKLEGTMSTSVAYLVSIGVSPRDIGPMVTQYPYLLGMRVGTM 251

Query: 276 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQ 335
           IKP V+YL S+G+P+  VAR++EKR +I+G+ LE+ VKPN+  L+ F V++  L  ++AQ
Sbjct: 252 IKPLVDYLISIGLPKKIVARMLEKRSYIVGYNLEETVKPNVDCLISFGVKKELLPLLIAQ 311

Query: 336 YAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVINHVDLLKTCG 395
           Y +I+G+ +++K+ TQ+      ++++ + F  ++EKMPQIVSL  + ++  ++ L    
Sbjct: 312 YPQILGLPVKAKMSTQQYFFSLKLKIDPEGFARVVEKMPQIVSLKQNVIMKPIEFLLGRA 371

Query: 396 FSLLQVKNMVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPR 455
           F +  +  MVV CPQ+L   +++MK+S+ F++ +MGRP+ +LV +P +FTY LES IKPR
Sbjct: 372 FQVEDIAKMVVRCPQILCSRVELMKNSYYFYKTEMGRPMKELVEYPEYFTYSLESRIKPR 431

Query: 456 HKKVVQKGISCSLSWLLNCSDEKFAERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSE 515
           ++K+  KGI  SL+W LNCSD++F ER+  + ID +     PTFDM   ++    E  ++
Sbjct: 432 YQKLQSKGIRSSLNWFLNCSDQRFEERLQGNFIDPD--TEGPTFDMGGKLEMPGGEIVTD 491

Query: 516 YEED 520
            EED
Sbjct: 492 EEED 493

BLAST of HG10022316 vs. TAIR 10
Match: AT2G21710.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 141.7 bits (356), Expect = 1.6e-33
Identity = 91/363 (25.07%), Postives = 173/363 (47.66%), Query Frame = 0

Query: 165 KKNMIPVLDYLGKLGVRKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPTDIPRV 224
           ++ +  +++YL   GVR+      + R P++L  S + ++   + +   M +   D   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 225 LERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLE 284
           +  YP+++GF     M   + YL   G++  E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 285 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDL 344
            LGIP+  + R++  +P +    LEK + P ++ L E  +    + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 345 ESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN-HVDLLKTCGFSLLQVKN 404
             K+      L +   +   + G +I   P ++  S    +  ++    + G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 405 MVVGCPQLLALNIDIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTIKPRHKKVVQKG 464
           M+   P LL  N+D ++  + + +  M RPL DL+ FP FF+Y LE  I PRH  +V+  
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 465 ISCSLSWLLNCSDEKFAERMSYDTIDMEE-------METTPTFDMNSLIQPRNDESDSEY 520
           ++  L ++L C+DE+F ER   D ++  E        E +   D N   Q      ++E 
Sbjct: 578 VNFKLRYMLACTDEEF-ERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEE 637

BLAST of HG10022316 vs. TAIR 10
Match: AT4G38160.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-32
Identity = 69/276 (25.00%), Postives = 146/276 (52.90%), Query Frame = 0

Query: 216 IKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 275
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 276 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 335
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 336 QYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN-HVDLLKT 395
           +   ++G  ++ +L      LKS + L++D   +++   PQ++    + ++  + D LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 396 CGFSLLQVKNMVVGCPQLLALNI-DIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTI 455
           CGF   Q+  MV G PQ+L  ++ + ++    F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 456 KPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTI 489
           + R K V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of HG10022316 vs. TAIR 10
Match: AT4G38160.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-32
Identity = 69/276 (25.00%), Postives = 146/276 (52.90%), Query Frame = 0

Query: 216 IKPTDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 275
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 276 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 335
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 336 QYAEIIGMDLESKLPTQRNLLKSLIELNDDNFGAIIEKMPQIVSLSSSAVIN-HVDLLKT 395
           +   ++G  ++ +L      LKS + L++D   +++   PQ++    + ++  + D LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 396 CGFSLLQVKNMVVGCPQLLALNI-DIMKHSFEFFQMDMGRPLDDLVTFPAFFTYGLESTI 455
           CGF   Q+  MV G PQ+L  ++ + ++    F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 456 KPRHKKVVQKGISCSLSWLLNCSDEKFAERMSYDTI 489
           + R K V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890870.11.1e-27393.27transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_0... [more]
XP_008465763.13.3e-27092.51PREDICTED: transcription termination factor MTERF4, chloroplastic [Cucumis melo]... [more]
XP_004143818.11.4e-26891.94transcription termination factor MTERF4, chloroplastic [Cucumis sativus] >KGN511... [more]
XP_023538106.18.3e-26689.62transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pe... [more]
XP_022938209.17.0e-26589.42transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_... [more]
Match NameE-valueIdentityDescription
Q9ZT963.8e-19767.42Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q6AUK61.7e-16063.68Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
B6TGN46.0e-15862.21Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
F4IHL32.3e-3225.07Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL61.5e-3125.00Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A1S3CR331.6e-27092.51transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A5A7VMZ01.6e-27092.51Transcription termination factor MTERF4 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0KRV76.6e-26991.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1[more]
A0A6J1FDE73.4e-26589.42transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1DUY13.5e-26288.65transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX... [more]
Match NameE-valueIdentityDescription
AT4G02990.12.7e-19867.42Mitochondrial transcription termination factor family protein [more]
AT2G44020.12.6e-12449.29Mitochondrial transcription termination factor family protein [more]
AT2G21710.11.6e-3325.07Mitochondrial transcription termination factor family protein [more]
AT4G38160.11.0e-3225.00Mitochondrial transcription termination factor family protein [more]
AT4G38160.21.0e-3225.00Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 367..397
e-value: 0.37
score: 19.8
coord: 402..433
e-value: 0.083
score: 22.0
coord: 186..217
e-value: 5.4E-4
score: 29.3
coord: 258..289
e-value: 1.1E-4
score: 31.6
coord: 222..253
e-value: 0.0053
score: 26.0
coord: 330..362
e-value: 670.0
score: 5.3
coord: 434..465
e-value: 0.04
score: 23.1
coord: 87..120
e-value: 640.0
score: 5.4
coord: 150..181
e-value: 0.0039
score: 26.4
coord: 294..325
e-value: 3.6E-4
score: 29.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 172..480
e-value: 1.0E-104
score: 349.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 31..512
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 136..275
e-value: 5.3E-25
score: 90.0
coord: 276..489
e-value: 6.1E-48
score: 165.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 496..520
NoneNo IPR availablePANTHERPTHR13068:SF109TRANSCRIPTION TERMINATION FACTOR MTERF4, CHLOROPLASTICcoord: 31..512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022316.1HG10022316.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0042794 plastid rRNA transcription
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0008380 RNA splicing
cellular_component GO:0009536 plastid
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003729 mRNA binding