HG10021959 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10021959
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptioniron-sulfur assembly protein IscA-like 2, mitochondrial
LocationChr05: 18982114 .. 18983755 (-)
RNA-Seq ExpressionHG10021959
SyntenyHG10021959
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCCTAGATCGCTAATTCAGAGGGTAACACCATACCTGGTGGCACGTATTCGAGAGAATCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCGTCTTTGTTGACATCTCCATCTCCATCTCCATCTTCTTCTGCACCTTTTGATACCATTCACATGACTGATAATTGCATTCGGGTATTCTATACATATATCCTTTCTGACAGACATTTGGCATAGGAATTCTTCATCCATTTCACTTTCATCCTCTAGTTTAATTCTTAATTTTCTGAATTATTCTTTTATGGTGCACCATTCAGGCCTATAGTTTATGGGTTATAGGATGCTAATACATTGTTGTGCCTTTTAGGTTCTTGAAATTCAAGTAAACAATTTGAATTTTGAGCATTTCTCTTGTTACCAGTTCGATCTATGTTGGGGTTCATAAATCGGTCTTGATTTACATTTTTGCTTAGTTTTGACATGCCTTGCAGAGAATGAAAGAATTGCAAGATCGCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGTTTAACAAATATTAACTCTGATTATTGCTTATTTGGGTTTTCACTGGTGTTGGATTTTACTTAACAAAGGTTTGTCCTCTCTCTTTTTATGTTTGCAGGGTGTATGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCATTCGTTGTAAGTGAAACTTATCTCTCATTATTGCTGGCTCACTGCTTTTGCTTAGATATTGTTTATTATTTTCACACCAGTGTGTGGACCATAGCAAATTATAGTTCTATGACATATATTCAAGTTAATGGAGTGATGATAAAGAAAATTGAAATACTGAAAAACCAACTTCCACTAACACCATCCTTGGAATAACTATCCTTTGACTGTTACACCATGCCATTTATGGAATCTCTCTCCCTTCATAAGTACTGTTAGGTATCTAGAGTATTATTTACGTTGAGAAATATACTGAGGTGCAAATCTTCCAGCCCTGGGCTGGGATATTCTCCACACTGTAGATCCGTACCTGAAATTACACATGATGACCACTCTCGTCTATCCTTCCACATACGTAATGTGCGTATGTGGCTCGGTTTCTGTCTGTCGGAGGTGGCATTTTCCATTTTACCCTTCCTTGAATATATGTGAACTGTAGGGGTATAACTTAGGCAAAATTTCCTATTTCACAATAGTATGAATATTGTTCTCATTCATTGTAGCTACACCAGTGTCATTTAAGAATGATGTGGTATAACAACAAAAAAAGATTATTTAGGAAGGAAAATTTACAATGTGAACAAAGAAAGTAGATAGTAATATTAAAGAATTCTTAAAAAATTTCCCTCAAAGCAACACATTTTACCCTAAAGTGACTATGGAATGTTATATATCCCATCATAATAGAATTACTAGTATTGCAGAAGTTGAATTCTTGTTACGTGCAGGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

mRNA sequence

ATGGCCCCTAGATCGCTAATTCAGAGGGTAACACCATACCTGGTGGCACGTATTCGAGAGAATCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCGTCTTTGTTGACATCTCCATCTCCATCTCCATCTTCTTCTGCACCTTTTGATACCATTCACATGACTGATAATTGCATTCGGAGAATGAAAGAATTGCAAGATCGCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGGTGTATGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCATTCGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Coding sequence (CDS)

ATGGCCCCTAGATCGCTAATTCAGAGGGTAACACCATACCTGGTGGCACGTATTCGAGAGAATCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCGTCTTTGTTGACATCTCCATCTCCATCTCCATCTTCTTCTGCACCTTTTGATACCATTCACATGACTGATAATTGCATTCGGAGAATGAAAGAATTGCAAGATCGCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGGTGTATGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCATTCGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Protein sequence

MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Homology
BLAST of HG10021959 vs. NCBI nr
Match: XP_038893859.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893860.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893861.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 1.0e-73
Identity = 149/161 (92.55%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MA RSLIQRVTPYLVARIRENQRLLSSSVSAHQEASS LT PSPSPS     DTIH+TDN
Sbjct: 1   MASRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSSLTCPSPSPS----VDTIHLTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQD KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKL+VDNISY
Sbjct: 61  CIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLIVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAF+VSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFIVSTNPSAVGGCSCKSSFMVKQ 157

BLAST of HG10021959 vs. NCBI nr
Match: XP_022964844.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP_023520276.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284.6 bits (727), Expect = 5.1e-73
Identity = 149/161 (92.55%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MAPR LIQR+TP+LVARIRENQRLLSSSVSAHQEASSLL   SPSPSSS P DTI MTDN
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLA--SPSPSSSGPIDTIQMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQ  KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+LVVDNISY
Sbjct: 61  CIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. NCBI nr
Match: XP_022970473.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 281.6 bits (719), Expect = 4.3e-72
Identity = 148/161 (91.93%), Postives = 151/161 (93.79%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MA R LIQR+TP+LVARIRENQRLLSSSVSAHQEASSLL   SPSPSSS P DTI MTDN
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLA--SPSPSSSGPIDTIQMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQ  KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+LVVDNISY
Sbjct: 61  CIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. NCBI nr
Match: XP_008457520.2 (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo] >XP_008457521.1 PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo] >XP_008457522.1 PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 9.7e-72
Identity = 145/161 (90.06%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           M PRSLIQRVTPYLVARI+ENQRLLSSS +A+QEASSL +SPSPSP  SAP DTIHMTD+
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSP--SAPVDTIHMTDS 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQD KEEKMLRLSVETGGCSGFQYVF+LD KT PDDR+YEKEGVKLVVDNISY
Sbjct: 61  CIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. NCBI nr
Match: KAG6583457.1 (Iron-sulfur assembly protein IscA-like 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 279.3 bits (713), Expect = 2.2e-71
Identity = 146/159 (91.82%), Postives = 150/159 (94.34%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MAPR LIQR+TP+LVARIRENQRLLSSSVSAHQEASSLL   SPSPSSS P DTI MTDN
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLA--SPSPSSSGPIDTIQMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQ  KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+LVVDNISY
Sbjct: 61  CIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 160
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157

BLAST of HG10021959 vs. ExPASy Swiss-Prot
Match: Q8LCY2 (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-54
Identity = 116/159 (72.96%), Postives = 133/159 (83.65%), Query Frame = 0

Query: 4   RSLIQRVTPYLVARIRENQRLLS-SSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCI 63
           RSL++RV PYL  RIREN RLL+ SS SA +EASS   S S  P SS+  D +H++DNCI
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASS---SSSSQPESSSN-DVVHLSDNCI 62

Query: 64  RRMKELQDRK-EEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYD 123
           RRMKELQ  + E+KMLRL VETGGCSGFQY F+LD++TNPDDRV+EK GVKLVVDN+SYD
Sbjct: 63  RRMKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYD 122

Query: 124 FVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 161
            VKGATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 LVKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

BLAST of HG10021959 vs. ExPASy Swiss-Prot
Match: Q2TBG7 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 7.6e-27
Identity = 71/151 (47.02%), Postives = 91/151 (60.26%), Query Frame = 0

Query: 10  VTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQ 69
           VTP+      +  RLL++S           +SP            IH+TD+C++R+ E+ 
Sbjct: 15  VTPW------QRSRLLAASRGPRALREVSFSSPEAGEGQ------IHLTDSCVQRLLEIT 74

Query: 70  DRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATID 129
           +  E   LRL VE GGCSGFQY F LD   NPDDRV+EK G ++VVD+ S  FVKGA +D
Sbjct: 75  EGSE--FLRLEVEGGGCSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVD 134

Query: 130 YVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 161
           + +ELIRS+F V  NP A  GCSC SSF VK
Sbjct: 135 FSQELIRSSFQVLNNPQAQQGCSCGSSFSVK 151

BLAST of HG10021959 vs. ExPASy Swiss-Prot
Match: Q9DCB8 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-26
Identity = 60/106 (56.60%), Postives = 76/106 (71.70%), Query Frame = 0

Query: 55  IHMTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLV 114
           I +TD+C++R+ E+ +  E   LRL VE GGCSGFQY F LD   NPDDRV+E+ G ++V
Sbjct: 50  IRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVV 109

Query: 115 VDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 161
           VD+ S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF VK
Sbjct: 110 VDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSVK 153

BLAST of HG10021959 vs. ExPASy Swiss-Prot
Match: Q86U28 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.7e-26
Identity = 70/140 (50.00%), Postives = 91/140 (65.00%), Query Frame = 0

Query: 23  RLLSSSV--SAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLS 82
           RLL++S+   A +EAS        S S  A    I +TD+C++R+ E+ +  E   LRL 
Sbjct: 24  RLLTASLGPQARREAS--------SSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQ 83

Query: 83  VETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFV 142
           VE GGCSGFQY F LD   NPDDRV+E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F 
Sbjct: 84  VEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQ 143

Query: 143 VSTNPSAVGGCSCKSSFMVK 161
           V  NP A  GCSC SSF +K
Sbjct: 144 VLNNPQAQQGCSCGSSFSIK 153

BLAST of HG10021959 vs. ExPASy Swiss-Prot
Match: Q5R788 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.7e-26
Identity = 70/140 (50.00%), Postives = 91/140 (65.00%), Query Frame = 0

Query: 23  RLLSSSV--SAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLS 82
           RLL++S+   A +EAS        S S  A    I +TD+C++R+ E+ +  E   LRL 
Sbjct: 24  RLLAASLGPQARREAS--------SSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQ 83

Query: 83  VETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFV 142
           VE GGCSGFQY F LD   NPDDRV+E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F 
Sbjct: 84  VEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQ 143

Query: 143 VSTNPSAVGGCSCKSSFMVK 161
           V  NP A  GCSC SSF +K
Sbjct: 144 VLNNPQAQQGCSCGSSFSIK 153

BLAST of HG10021959 vs. ExPASy TrEMBL
Match: A0A6J1HPD1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464825 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.5e-73
Identity = 149/161 (92.55%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MAPR LIQR+TP+LVARIRENQRLLSSSVSAHQEASSLL   SPSPSSS P DTI MTDN
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLA--SPSPSSSGPIDTIQMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQ  KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+LVVDNISY
Sbjct: 61  CIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. ExPASy TrEMBL
Match: A0A6J1I3Y1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111469443 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.1e-72
Identity = 148/161 (91.93%), Postives = 151/161 (93.79%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           MA R LIQR+TP+LVARIRENQRLLSSSVSAHQEASSLL   SPSPSSS P DTI MTDN
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLA--SPSPSSSGPIDTIQMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQ  KEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+LVVDNISY
Sbjct: 61  CIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. ExPASy TrEMBL
Match: A0A1S3C5P3 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497193 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.7e-72
Identity = 145/161 (90.06%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           M PRSLIQRVTPYLVARI+ENQRLLSSS +A+QEASSL +SPSPSP  SAP DTIHMTD+
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSP--SAPVDTIHMTDS 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQD KEEKMLRLSVETGGCSGFQYVF+LD KT PDDR+YEKEGVKLVVDNISY
Sbjct: 61  CIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. ExPASy TrEMBL
Match: A0A0A0LXX8 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 8.8e-71
Identity = 144/161 (89.44%), Postives = 149/161 (92.55%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           M PRSLIQRVTPYLVARI+ENQRLLSSS SA+QEA  L    SPSPS SAP DTIHMTD+
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPL----SPSPSPSAPVDTIHMTDS 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CIRRMKELQD KEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGVKLVVDNISY
Sbjct: 61  CIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

BLAST of HG10021959 vs. ExPASy TrEMBL
Match: A0A6J1DIX6 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111020448 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.9e-69
Identity = 139/161 (86.34%), Postives = 148/161 (91.93%), Query Frame = 0

Query: 1   MAPRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDN 60
           M PR LIQR+TP+ +ARIRENQRLLSSSVSAHQE  SLLT  SPSPSSS   DTIHMTDN
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLT--SPSPSSSVLIDTIHMTDN 60

Query: 61  CIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISY 120
           CI++MKELQ+  EEKMLRLSVETGGCSGFQYVF+LDDKTNPDDRVYEK+GVKLVVDNISY
Sbjct: 61  CIQKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISY 120

Query: 121 DFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 162
           DFVKGATIDY EELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 DFVKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

BLAST of HG10021959 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.0e-55
Identity = 116/159 (72.96%), Postives = 133/159 (83.65%), Query Frame = 0

Query: 4   RSLIQRVTPYLVARIRENQRLLS-SSVSAHQEASSLLTSPSPSPSSSAPFDTIHMTDNCI 63
           RSL++RV PYL  RIREN RLL+ SS SA +EASS   S S  P SS+  D +H++DNCI
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASS---SSSSQPESSSN-DVVHLSDNCI 62

Query: 64  RRMKELQDRK-EEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVDNISYD 123
           RRMKELQ  + E+KMLRL VETGGCSGFQY F+LD++TNPDDRV+EK GVKLVVDN+SYD
Sbjct: 63  RRMKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYD 122

Query: 124 FVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 161
            VKGATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 LVKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

BLAST of HG10021959 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 68.6 bits (166), Expect = 5.4e-12
Identity = 37/125 (29.60%), Postives = 68/125 (54.40%), Query Frame = 0

Query: 34  EASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVF 93
           +AS +L + +     +     + +TD    R+  L  ++++  LRL V+  GC+G  Y  
Sbjct: 2   KASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTL 61

Query: 94  DLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSC 153
           +  D+    D + E++GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C
Sbjct: 62  NYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGC 121

Query: 154 KSSFM 159
             SFM
Sbjct: 122 GESFM 125

BLAST of HG10021959 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 36/101 (35.64%), Postives = 59/101 (58.42%), Query Frame = 0

Query: 57  MTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLVVD 116
           ++D    R+++L   +++  LRL+VE  GC+G  YV +   +    D V E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 117 NISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSF 158
             +   V G  +D+V++ +RS FV   NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

BLAST of HG10021959 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 64.7 bits (156), Expect = 7.8e-11
Identity = 38/131 (29.01%), Postives = 70/131 (53.44%), Query Frame = 0

Query: 34  EASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVF 93
           +AS +L + +     +     + +TD    R+  L  ++++  LRL V+  GC+G  Y  
Sbjct: 2   KASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTL 61

Query: 94  DLDDKTNPD------DRVYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSA 153
           +   KT+ +      D + E++GV+++V+  +   V G  +D+V++ +RS FV   NP++
Sbjct: 62  NYAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNS 121

Query: 154 VGGCSCKSSFM 159
            G C C  SFM
Sbjct: 122 QGQCGCGESFM 131

BLAST of HG10021959 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 51.2 bits (121), Expect = 8.9e-07
Identity = 26/103 (25.24%), Postives = 55/103 (53.40%), Query Frame = 0

Query: 34  EASSLLTSPSPSPSSSAPFDTIHMTDNCIRRMKELQDRKEEKMLRLSVETGGCSGFQYVF 93
           +AS +L + +     +     + +TD    R+  L  ++++  LRL V+  GC+G  Y  
Sbjct: 2   KASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTL 61

Query: 94  DLDDKTNPDDRVYEKEGVKLVVDNISYDFVKGATIDYVEELIR 137
           +  D+    D + E++GV+++V+  +   V G  +D+V++ +R
Sbjct: 62  NYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893859.11.0e-7392.55iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_... [more]
XP_022964844.15.1e-7392.55iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP... [more]
XP_022970473.14.3e-7291.93iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima][more]
XP_008457520.29.7e-7290.06PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis melo... [more]
KAG6583457.12.2e-7191.82Iron-sulfur assembly protein IscA-like 2, mitochondrial, partial [Cucurbita argy... [more]
Match NameE-valueIdentityDescription
Q8LCY21.5e-5472.96Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ... [more]
Q2TBG77.6e-2747.02Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I... [more]
Q9DCB81.3e-2656.60Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G... [more]
Q86U281.7e-2650.00Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN... [more]
Q5R7881.7e-2650.00Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1HPD12.5e-7392.55iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX... [more]
A0A6J1I3Y12.1e-7291.93iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3... [more]
A0A1S3C5P34.7e-7290.06iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 ... [more]
A0A0A0LXX88.8e-7189.44Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290... [more]
A0A6J1DIX64.9e-6986.34iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia O... [more]
Match NameE-valueIdentityDescription
AT5G03905.11.0e-5572.96Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.15.4e-1229.60Iron-sulphur cluster biosynthesis family protein [more]
AT2G36260.11.2e-1135.64Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.37.8e-1129.01Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.28.9e-0725.24Iron-sulphur cluster biosynthesis family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035903HesB-like domain superfamilyGENE3D2.60.300.12coord: 54..161
e-value: 4.0E-35
score: 122.2
IPR035903HesB-like domain superfamilySUPERFAMILY89360HesB-like domaincoord: 54..147
IPR016092FeS cluster insertion proteinTIGRFAMTIGR00049TIGR00049coord: 55..159
e-value: 1.9E-34
score: 116.1
IPR000361FeS cluster biogenesisPFAMPF01521Fe-S_biosyncoord: 55..154
e-value: 3.1E-16
score: 59.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..54
NoneNo IPR availablePANTHERPTHR43011:SF4BNACNNG10620D PROTEINcoord: 4..161
NoneNo IPR availablePANTHERPTHR43011IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIALcoord: 4..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10021959.1HG10021959.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0106035 protein maturation by [4Fe-4S] cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding