HG10021767 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10021767
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionStructure-specific endonuclease subunit SLX1 homolog
LocationChr05: 16374091 .. 16376399 (-)
RNA-Seq ExpressionHG10021767
SyntenyHG10021767
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGAAGGGGAAAGAGAAATCGGAAGTCTCTAAAACCATGGCTGTAACAGAGGAGGAGAAGGAAGACGATGAAGAAGAAGATGAAGAAGAAAGGAGCAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTACAAAGGCCATACCTATATCGGGTTTGAATCTTTCCCACTTCATTCGAGCATTTTTCTGTTCTTGCATCTCTAGATCGGATCTTCTATTTGCTTTGTAATTTCCCACTTTTCGGATTTAATGCTCTGCTTCCCTATACTGGAAATTTCTTTTCCTTCCTGCGTAAAGAAATATGGGAAATGCGTAAGTCGATCTGCAGTTGGATTGATTGGATGATTGTTTATATGGAATTTTAGATTTACAGTGAACCCGAAACGCAGAATCAGACAGCACAATGGTGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGGTTACATTTCTAGATTTTGAGCTTTAAATTTAGTTTGATTGTGCGTCTTTTATACTCGATTGTCTTCAACTAGCTGAATGATACTTCGATGATTATGCAAATGTTAAGATGTTAGAGTACATATTATGGATTTTTGGGGTTAAAGAATTTACTAAATTGTGTTGCTGGTTTCCCTCTTTAGTCATTTATTTTCTTGTGAGTCTCCATCAGTTCTAGAATTTGATGTTCTAACTATGGATGCAGTTTGAATGGGCGTGGCAGCATCCTAGTGAGTCATTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGTGTAGGTATGACGTATGAGAAAATTTACACGATTCTATCGAACTTTTTCTGTTAATTGTGTTGGAAATGTGAATATAATGTATAAATAAAGACAATACTCTTATAACTTTGAGGGTTTGGGGGGCGAAACTAAAGTTTATGTCTGATAGGGGCAATATCTTACTATTGTGATTTATGAAGGAGCAACAAACTGATGTAATTTCAATCAAATTTGTCTGCCAAACTTTCTCTTTATTCACTTTTTTCTCCTTATCCTTTCAGTTTGAATATAACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCCATGAGCTTCCATGCTATTCCGAAGGAGATCAATGTGTACTTGAAAATGAAGAGGATTTGGAGTATAATAGAGAATGTGAAGAAATTTGTAGTTTTCGAGTATATGGATCGATGAAAGAAGTTCCAAATGAAGTTCCTAAAAAATTAATGGATTCTACTAGTACAGATGGAAGACCTCATGAATTGCGTGGATGCGATAAAGAATTTGTGAACAATGAACGAGTGCCACCTTCTTTGTGTACTCCATCTGATATTGATGCGGGTATGTCTCATGAATTGTGTGGATGCGATAAAGAATTTGAGAACAATGAACGAGTGCCATGTTCTTCGTGTACTCCATCTTATATTGATGCGGGTATGTCTTATGACTTGCGTGGATGTGATGAAGAACTCGAGGAGGATGAAAGAGAGCCAGCTTCATGTGCTCAGTCTTGTATTGTAGCAGCAGGTACATCTAGGACTGAAATTATCATTGATGATGGAGATGAAGACCAATTAGAAGGGAATGGTATGAACTTGCAGAAACAACTTGGTAGGAAGAATTTAACATCAGGAATAGCTTCTGAAATCAACAAAGTTTCAAGGTGTACTAATGAGCTTCCTCCTGTTGAATATGAGATTATAGATGTGTCTACCCCGTCTCCCGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCTGGTAGGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAGAATGATGGATGGATTTGGATTCTGCATATAATATAAATTGGTTTCGAATGACTTTCAAGTGTGAAAGAAAAGTGCATTTTGCCATGAGAAGGTAGTATTTTCAACCCTTTCAGAGGGCAACGTGGGCTCAAGTTTGGTTGACCAACAACATGTTGTCAAGTTATGTATAATAATAATGGCCTTTCTTTCAAAAGCCATTTTTAAGGGAATCAAGTGTGAAAATGATATTCAAATGGCTTGTGTATTAGAAGAGAACGTGGTATCAATTAACTCCTTCCTTCCAAACGATTGGGCTCAAAGTTGA

mRNA sequence

ATGCGGAAGGGGAAAGAGAAATCGGAAGTCTCTAAAACCATGGCTGTAACAGAGGAGGAGAAGGAAGACGATGAAGAAGAAGATGAAGAAGAAAGGAGCAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTACAAAGGCCATACCTATATCGGATTTACAGTGAACCCGAAACGCAGAATCAGACAGCACAATGGTGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAGTGAGTCATTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGTGTAGTTTGAATATAACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCCATGAGCTTCCATGCTATTCCGAAGGAGATCAATGTGTACTTGAAAATGAAGAGGATTTGGAGTATAATAGAGAATGTGAAGAAATTTGTAGTTTTCGAGTATATGGATCGATGAAAGAAGTTCCAAATGAAGTTCCTAAAAAATTAATGGATTCTACTAGTACAGATGGAAGACCTCATGAATTGCGTGGATGCGATAAAGAATTTGTGAACAATGAACGAGTGCCACCTTCTTTGTGTACTCCATCTGATATTGATGCGGGTATGTCTCATGAATTGTGTGGATGCGATAAAGAATTTGAGAACAATGAACGAGTGCCATGTTCTTCGTGTACTCCATCTTATATTGATGCGGGTATGTCTTATGACTTGCGTGGATGTGATGAAGAACTCGAGGAGGATGAAAGAGAGCCAGCTTCATGTGCTCAGTCTTGTATTGTAGCAGCAGGTACATCTAGGACTGAAATTATCATTGATGATGGAGATGAAGACCAATTAGAAGGGAATGGTATGAACTTGCAGAAACAACTTGGTAGGAAGAATTTAACATCAGGAATAGCTTCTGAAATCAACAAAGTTTCAAGGTGTACTAATGAGCTTCCTCCTGTTGAATATGAGATTATAGATGTGTCTACCCCGTCTCCCGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCTGAGGGCAACGTGGGCTCAAGTTTGGTTGACCAACAACATGTTGTCAAGTTATGTATAATAATAATGGCCTTTCTTTCAAAAGCCATTTTTAAGGGAATCAAGTGTGAAAATGATATTCAAATGGCTTGTGTATTAGAAGAGAACGTGGTATCAATTAACTCCTTCCTTCCAAACGATTGGGCTCAAAGTTGA

Coding sequence (CDS)

ATGCGGAAGGGGAAAGAGAAATCGGAAGTCTCTAAAACCATGGCTGTAACAGAGGAGGAGAAGGAAGACGATGAAGAAGAAGATGAAGAAGAAAGGAGCAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTACAAAGGCCATACCTATATCGGATTTACAGTGAACCCGAAACGCAGAATCAGACAGCACAATGGTGAAATTAGATGCGGTGCGTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTTTTGTGCATTTATGGCTTCCCCACTAATGTCTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAGTGAGTCATTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGTGTAGTTTGAATATAACTGTAAACTACTTCTCAACAAAGTACATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCCATGAGCTTCCATGCTATTCCGAAGGAGATCAATGTGTACTTGAAAATGAAGAGGATTTGGAGTATAATAGAGAATGTGAAGAAATTTGTAGTTTTCGAGTATATGGATCGATGAAAGAAGTTCCAAATGAAGTTCCTAAAAAATTAATGGATTCTACTAGTACAGATGGAAGACCTCATGAATTGCGTGGATGCGATAAAGAATTTGTGAACAATGAACGAGTGCCACCTTCTTTGTGTACTCCATCTGATATTGATGCGGGTATGTCTCATGAATTGTGTGGATGCGATAAAGAATTTGAGAACAATGAACGAGTGCCATGTTCTTCGTGTACTCCATCTTATATTGATGCGGGTATGTCTTATGACTTGCGTGGATGTGATGAAGAACTCGAGGAGGATGAAAGAGAGCCAGCTTCATGTGCTCAGTCTTGTATTGTAGCAGCAGGTACATCTAGGACTGAAATTATCATTGATGATGGAGATGAAGACCAATTAGAAGGGAATGGTATGAACTTGCAGAAACAACTTGGTAGGAAGAATTTAACATCAGGAATAGCTTCTGAAATCAACAAAGTTTCAAGGTGTACTAATGAGCTTCCTCCTGTTGAATATGAGATTATAGATGTGTCTACCCCGTCTCCCGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCTGAGGGCAACGTGGGCTCAAGTTTGGTTGACCAACAACATGTTGTCAAGTTATGTATAATAATAATGGCCTTTCTTTCAAAAGCCATTTTTAAGGGAATCAAGTGTGAAAATGATATTCAAATGGCTTGTGTATTAGAAGAGAACGTGGTATCAATTAACTCCTTCCTTCCAAACGATTGGGCTCAAAGTTGA

Protein sequence

MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVSPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMDSTSTDGRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYIDAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQLGRKNLTSGIASEINKVSRCTNELPPVEYEIIDVSTPSPDCRTSSHRFKRRVTSEGNVGSSLVDQQHVVKLCIIIMAFLSKAIFKGIKCENDIQMACVLEENVVSINSFLPNDWAQS
Homology
BLAST of HG10021767 vs. NCBI nr
Match: XP_038894793.1 (structure-specific endonuclease subunit SLX1 [Benincasa hispida])

HSP 1 Score: 676.8 bits (1745), Expect = 1.4e-190
Identity = 341/414 (82.37%), Postives = 359/414 (86.71%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MRK KE+ E+SKTMAV EEEKEDDE+E+EEE SNEVNGFFSCYLLASACPRYKGHTYIGF
Sbjct: 1   MRKRKERPEISKTMAVAEEEKEDDEDEEEEEGSNEVNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLP+HMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPKHMKVQV 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI+ELPCYSEGDQCVLENE+D EYNRECEEICSFRVYGSMKEV NEVP+KLMD   STD
Sbjct: 181 SPINELPCYSEGDQCVLENEDDWEYNRECEEICSFRVYGSMKEVSNEVPQKLMDHQASTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRP EL GCDKEF NNE+VPP                               SSC PSYI
Sbjct: 241 GRPRELHGCDKEFENNEQVPP-------------------------------SSCNPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           DAGMSYDL GCDEELEEDEREPASCA SCIV    SRTEIIIDDGDEDQ+EG GMNL++Q
Sbjct: 301 DAGMSYDLHGCDEELEEDEREPASCAPSCIV-TDISRTEIIIDDGDEDQIEGTGMNLEQQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNELPPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
           L RKNL+SGIASEI+KVSRC  ELPPVEYE+IDVSTPSPDCRT+SHR KR+VTS
Sbjct: 361 LCRKNLSSGIASEISKVSRCNIELPPVEYEVIDVSTPSPDCRTNSHRVKRKVTS 382

BLAST of HG10021767 vs. NCBI nr
Match: XP_008457313.1 (PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo])

HSP 1 Score: 629.4 bits (1622), Expect = 2.5e-176
Identity = 330/417 (79.14%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMA--VTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYI 60
           MRK KEK E+ KTMA  V +EEKEDD+E  EEER N VNGFFSCYLLASACPR+KGHTYI
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDE--EEERGNGVNGFFSCYLLASACPRFKGHTYI 60

Query: 61  GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLA 120
           GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLA
Sbjct: 61  GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLA 120

Query: 121 VRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKV 180
           VRSAAATFKSLSGVANKVKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKV
Sbjct: 121 VRSAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKV 180

Query: 181 QVSPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STS 240
           QVSPI ELPCYSEGDQ VLENE+D EY+RE EEICSF+VYGSMKEV NEVP+KLMD  T 
Sbjct: 181 QVSPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTG 240

Query: 241 TDGRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPS 300
           TDGRP                PP               L GCDKE E NE+VP SSCTPS
Sbjct: 241 TDGRP----------------PP--------------VLRGCDKELETNEQVPPSSCTPS 300

Query: 301 YIDAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQ 360
           Y+D GMSYDL  CDE LE DERE ASC QSC V AGTSRTEIIIDDG+E+Q EG+GMNLQ
Sbjct: 301 YVDVGMSYDLCACDEGLENDEREAASCGQSCTV-AGTSRTEIIIDDGEENQFEGSGMNLQ 360

Query: 361 KQLGRKNLTSGIASEINKVSRCTNEL-PPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
           +Q GRKNLTSGIASEI+KVSRC+N L P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 361 EQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 384

BLAST of HG10021767 vs. NCBI nr
Match: KAA0060004.1 (Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ97262.1 Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa])

HSP 1 Score: 617.5 bits (1591), Expect = 9.9e-173
Identity = 319/400 (79.75%), Postives = 337/400 (84.25%), Query Frame = 0

Query: 16  VTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEI 75
           V +EEKEDD+E  EEER N VNGFFSCYLLASACPR+KGHTYIGFTVNPKRRIRQHNGEI
Sbjct: 5   VQDEEKEDDDE--EEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEI 64

Query: 76  RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANK 135
           RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLAVRSAAATFKSLSGVANK
Sbjct: 65  RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANK 124

Query: 136 VKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVSPIHELPCYSEGDQC 195
           VKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQVSPI ELPCYSEGDQ 
Sbjct: 125 VKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQG 184

Query: 196 VLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTDGRPHELRGCDKEFVN 255
           VLENE+D EY+RE EEICSF+VYGSMKEV NEVP+KLMD  T TDGRP            
Sbjct: 185 VLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRP------------ 244

Query: 256 NERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYIDAGMSYDLRGCDEEL 315
               PP               L GCDKE E NE+VP SSCTPSY+D GMSYDL  CDE L
Sbjct: 245 ----PP--------------VLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGL 304

Query: 316 EEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQLGRKNLTSGIASEIN 375
           E DERE ASC QSC V AGTSRTEIIIDDG+E+Q EG+GMNLQ+Q GRKNLTSGIASEI+
Sbjct: 305 ENDEREAASCGQSCTV-AGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEIS 364

Query: 376 KVSRCTNEL-PPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
           KVSRC+N L P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 365 KVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 371

BLAST of HG10021767 vs. NCBI nr
Match: XP_004153269.1 (structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus])

HSP 1 Score: 614.8 bits (1584), Expect = 6.4e-172
Identity = 324/415 (78.07%), Postives = 342/415 (82.41%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MRK KEK E+ KT   T+EEKEDDE   EEER NEVNGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKEKPEICKT---TDEEKEDDE---EEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK+MKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI+ELPCYSEGDQ +LENE D EYNRE EEIC FRVYGSMKEV NEVP+KLMD  T TD
Sbjct: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRP                               H L GCDKE E NE+VP SSCTPSYI
Sbjct: 241 GRP------------------------------PHVLRGCDKELETNEQVPPSSCTPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           D GMSYDL  CDE LE DERE ASC QSCIV AGTSRTEI+IDD +E+QLEG+ MNLQ+Q
Sbjct: 301 DVGMSYDLCACDEGLENDEREAASCGQSCIV-AGTSRTEIVIDDEEENQLEGSSMNLQEQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNE-LPPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
            GR+NLTSGIASEI+KVSR  N  +P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 361 PGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 378

BLAST of HG10021767 vs. NCBI nr
Match: XP_022970648.1 (uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970649.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970650.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970651.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima])

HSP 1 Score: 590.1 bits (1520), Expect = 1.7e-164
Identity = 307/432 (71.06%), Postives = 341/432 (78.94%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MR+ KEK E+ KTMAV EE+KEDDE   +EER NE NGFFSCYLLASACPRYKGHTYIGF
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDE---DEERGNEGNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSG+ANK+KLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLP HM+VQ+
Sbjct: 121 SAAATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQI 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI ELPCYSEGD+CVLENE D EYN+E EE+CS +V+G M EVPNE+P+ LMD ST TD
Sbjct: 181 SPIDELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRPHELR                               G D+E ++NE+VP SSCTPSYI
Sbjct: 241 GRPHELR-------------------------------GSDEELDDNEQVPHSSCTPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           D G+SYDLRGCDE+      EPA CAQ C V      TEIIIDDG+E+QLEG+GM LQ+Q
Sbjct: 301 DVGVSYDLRGCDEQ------EPALCAQPCTVEGW---TEIIIDDGEENQLEGSGMKLQQQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNELPPVEYEIIDVSTPSPDCRTSSHRFKRRVTSE----- 420
            GRK   SGIA+EI+KVS     LPPVEYE+IDVSTPSP CRTSSH+FKRR+ ++     
Sbjct: 361 PGRKKNLSGIATEISKVS---YGLPPVEYEVIDVSTPSPVCRTSSHKFKRRLITDSIFNP 386

Query: 421 --GNVGSSLVDQ 425
             GNVGS+LVDQ
Sbjct: 421 FRGNVGSNLVDQ 386

BLAST of HG10021767 vs. ExPASy Swiss-Prot
Match: Q8BX32 (Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.3e-29
Identity = 70/178 (39.33%), Postives = 97/178 (54.49%), Query Frame = 0

Query: 39  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 98
           FF  YLL    PR++G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL I+GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLIIHGF 69

Query: 99  PTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 158
           P+ V+AL+FEWAWQHP  S  +       +S +  A  +++   ML +P W  L +T+ +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEAAFAFHLRVLAHMLRVPPWVRLPLTLRW 129

Query: 159 FSTKYMKNAAGCPSLPEHMKVQVSP------IHELPCYSEGDQCVLENEEDLEYNREC 210
               +      CP+ P HM +   P      + + P  SE D    E + DL     C
Sbjct: 130 LRPDFRHEL--CPAPPAHMPIAFGPPPPQPLVPKRPAVSEADS---ERQLDLGTKARC 182

BLAST of HG10021767 vs. ExPASy Swiss-Prot
Match: Q5PQP5 (Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.3e-29
Identity = 69/178 (38.76%), Postives = 95/178 (53.37%), Query Frame = 0

Query: 39  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 98
           FF  YLL    PR++G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL ++GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLILHGF 69

Query: 99  PTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 158
           P+ V+AL+FEWAWQHP  S  +       +S +     +++   ML +P W  L +TV +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEASFTFHLRVLAHMLRVPPWVRLPLTVRW 129

Query: 159 FSTKYMKNAAGCPSLPEHMKVQVSP------IHELPCYSEGDQCVLENEEDLEYNREC 210
               +      CP+ P HM +   P      + + P  SE D    E   DL     C
Sbjct: 130 LRPDFRHEL--CPAPPPHMPIAFGPPPPQPLVPKRPAASEADS---ERRLDLGAKASC 182

BLAST of HG10021767 vs. ExPASy Swiss-Prot
Match: Q32PI0 (Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 9.1e-28
Identity = 60/145 (41.38%), Postives = 85/145 (58.62%), Query Frame = 0

Query: 39  FFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 98
           FF  YLL    PR++G  Y+GFTVNP RR++QHN G  + GAWRT  + PWEMVL ++GF
Sbjct: 6   FFGVYLLYCLNPRHRGRVYVGFTVNPARRVQQHNGGRKKGGAWRTSGRGPWEMVLIVHGF 65

Query: 99  PTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 158
           P+ V+AL+FEWAWQHP  S  +       +  +  A  +++   ML  P W  L +T+ +
Sbjct: 66  PSAVAALRFEWAWQHPQASRRLTHVGRRLRGEATFAFHLRVLAHMLRAPPWVRLPLTLRW 125

Query: 159 FSTKYMKNAAGCPSLPEHMKVQVSP 183
               + ++   CP  P HM +   P
Sbjct: 126 LRADFRQDL--CPPPPPHMPLAFGP 148

BLAST of HG10021767 vs. ExPASy Swiss-Prot
Match: Q0IH86 (Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.5e-27
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0

Query: 35  EVNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLC 94
           EV GF+  YLL    P+YKG  YIGFTVNP+RRI+QHN G+ + GAW+T  + PW+MVL 
Sbjct: 4   EVEGFYGVYLLFCTNPKYKGRIYIGFTVNPERRIQQHNGGKHKGGAWKTSGRGPWDMVLI 63

Query: 95  IYGFPTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNI 154
           ++GFP +++AL+FEWAWQHP  S  +       K  S     + +   ML +  W  L +
Sbjct: 64  VHGFPNDIAALRFEWAWQHPHVSRRLTHVPRKTKKQSSFDFHLLVLCHMLRVAPWNRLPL 123

Query: 155 TVNYFSTKYMKNAAGCPSLPEHMKVQVSPIHELP 188
           T+ +   +Y +        P HM +    +   P
Sbjct: 124 TLRWLRQEYRRELPLLLQPPLHMPLAFGQVRARP 157

BLAST of HG10021767 vs. ExPASy Swiss-Prot
Match: A7STV9 (Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis OX=45351 GN=v1g174298 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 8.5e-26
Identity = 61/149 (40.94%), Postives = 88/149 (59.06%), Query Frame = 0

Query: 37  NGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEI-RCGAWRTKRKRPWEMVLCIY 96
           +GF   YLL    P++KGHTYIG+TVNP RRI+QHNG + + GA++T RK+PW M+L ++
Sbjct: 8   SGFHGVYLLYCVNPKFKGHTYIGYTVNPNRRIKQHNGGVDKGGAYKTSRKKPWNMILIVH 67

Query: 97  GFPTNVSALQFEWAWQHPSESLAVRSAAATFKSLS-GVANKVKLAYTMLTLPAWCSLNIT 156
           GFP ++ ALQFEWAWQ P+ S  ++SAA   K     V    ++   +L +  W  L + 
Sbjct: 68  GFPNDIIALQFEWAWQKPTVSRRLKSAATKKKPRERPVQYCFRILSELLRVGPWNRLPLH 127

Query: 157 VNYFSTKYMKNAAGCPSLPEHMKVQVSPI 184
           + +   +Y          P HM V   P+
Sbjct: 128 IRWLMREYEMEFDPRCLPPFHMTVVYGPL 156

BLAST of HG10021767 vs. ExPASy TrEMBL
Match: A0A1S3C574 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=LOC103497035 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.2e-176
Identity = 330/417 (79.14%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMA--VTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYI 60
           MRK KEK E+ KTMA  V +EEKEDD+E  EEER N VNGFFSCYLLASACPR+KGHTYI
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDE--EEERGNGVNGFFSCYLLASACPRFKGHTYI 60

Query: 61  GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLA 120
           GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLA
Sbjct: 61  GFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLA 120

Query: 121 VRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKV 180
           VRSAAATFKSLSGVANKVKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKV
Sbjct: 121 VRSAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKV 180

Query: 181 QVSPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STS 240
           QVSPI ELPCYSEGDQ VLENE+D EY+RE EEICSF+VYGSMKEV NEVP+KLMD  T 
Sbjct: 181 QVSPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTG 240

Query: 241 TDGRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPS 300
           TDGRP                PP               L GCDKE E NE+VP SSCTPS
Sbjct: 241 TDGRP----------------PP--------------VLRGCDKELETNEQVPPSSCTPS 300

Query: 301 YIDAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQ 360
           Y+D GMSYDL  CDE LE DERE ASC QSC V AGTSRTEIIIDDG+E+Q EG+GMNLQ
Sbjct: 301 YVDVGMSYDLCACDEGLENDEREAASCGQSCTV-AGTSRTEIIIDDGEENQFEGSGMNLQ 360

Query: 361 KQLGRKNLTSGIASEINKVSRCTNEL-PPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
           +Q GRKNLTSGIASEI+KVSRC+N L P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 361 EQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 384

BLAST of HG10021767 vs. ExPASy TrEMBL
Match: A0A5D3BBR0 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00560 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.8e-173
Identity = 319/400 (79.75%), Postives = 337/400 (84.25%), Query Frame = 0

Query: 16  VTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEI 75
           V +EEKEDD+E  EEER N VNGFFSCYLLASACPR+KGHTYIGFTVNPKRRIRQHNGEI
Sbjct: 5   VQDEEKEDDDE--EEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEI 64

Query: 76  RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANK 135
           RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLAVRSAAATFKSLSGVANK
Sbjct: 65  RCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANK 124

Query: 136 VKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQVSPIHELPCYSEGDQC 195
           VKLAYTMLTLPAWC LNITVNYFSTK+MKNAAGCPSLPEHMKVQVSPI ELPCYSEGDQ 
Sbjct: 125 VKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQG 184

Query: 196 VLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTDGRPHELRGCDKEFVN 255
           VLENE+D EY+RE EEICSF+VYGSMKEV NEVP+KLMD  T TDGRP            
Sbjct: 185 VLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRP------------ 244

Query: 256 NERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYIDAGMSYDLRGCDEEL 315
               PP               L GCDKE E NE+VP SSCTPSY+D GMSYDL  CDE L
Sbjct: 245 ----PP--------------VLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGL 304

Query: 316 EEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQLGRKNLTSGIASEIN 375
           E DERE ASC QSC V AGTSRTEIIIDDG+E+Q EG+GMNLQ+Q GRKNLTSGIASEI+
Sbjct: 305 ENDEREAASCGQSCTV-AGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEIS 364

Query: 376 KVSRCTNEL-PPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
           KVSRC+N L P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 365 KVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 371

BLAST of HG10021767 vs. ExPASy TrEMBL
Match: A0A0A0M0Y6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G533630 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 3.1e-172
Identity = 324/415 (78.07%), Postives = 342/415 (82.41%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MRK KEK E+ KT   T+EEKEDDE   EEER NEVNGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKEKPEICKT---TDEEKEDDE---EEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK+MKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI+ELPCYSEGDQ +LENE D EYNRE EEIC FRVYGSMKEV NEVP+KLMD  T TD
Sbjct: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRP                               H L GCDKE E NE+VP SSCTPSYI
Sbjct: 241 GRP------------------------------PHVLRGCDKELETNEQVPPSSCTPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           D GMSYDL  CDE LE DERE ASC QSCIV AGTSRTEI+IDD +E+QLEG+ MNLQ+Q
Sbjct: 301 DVGMSYDLCACDEGLENDEREAASCGQSCIV-AGTSRTEIVIDDEEENQLEGSSMNLQEQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNE-LPPVEYEIIDVSTPSPDCRTSSHRFKRRVTS 414
            GR+NLTSGIASEI+KVSR  N  +P VEYE+IDVSTPSPDCRTSSHRFKRRVTS
Sbjct: 361 PGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTS 378

BLAST of HG10021767 vs. ExPASy TrEMBL
Match: A0A6J1I681 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 8.2e-165
Identity = 307/432 (71.06%), Postives = 341/432 (78.94%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MR+ KEK E+ KTMAV EE+KEDDE   +EER NE NGFFSCYLLASACPRYKGHTYIGF
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDE---DEERGNEGNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSG+ANK+KLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLP HM+VQ+
Sbjct: 121 SAAATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQI 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI ELPCYSEGD+CVLENE D EYN+E EE+CS +V+G M EVPNE+P+ LMD ST TD
Sbjct: 181 SPIDELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRPHELR                               G D+E ++NE+VP SSCTPSYI
Sbjct: 241 GRPHELR-------------------------------GSDEELDDNEQVPHSSCTPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           D G+SYDLRGCDE+      EPA CAQ C V      TEIIIDDG+E+QLEG+GM LQ+Q
Sbjct: 301 DVGVSYDLRGCDEQ------EPALCAQPCTVEGW---TEIIIDDGEENQLEGSGMKLQQQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNELPPVEYEIIDVSTPSPDCRTSSHRFKRRVTSE----- 420
            GRK   SGIA+EI+KVS     LPPVEYE+IDVSTPSP CRTSSH+FKRR+ ++     
Sbjct: 361 PGRKKNLSGIATEISKVS---YGLPPVEYEVIDVSTPSPVCRTSSHKFKRRLITDSIFNP 386

Query: 421 --GNVGSSLVDQ 425
             GNVGS+LVDQ
Sbjct: 421 FRGNVGSNLVDQ 386

BLAST of HG10021767 vs. ExPASy TrEMBL
Match: A0A6J1HKB9 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111464889 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 2.5e-161
Identity = 305/432 (70.60%), Postives = 338/432 (78.24%), Query Frame = 0

Query: 1   MRKGKEKSEVSKTMAVTEEEKEDDEEEDEEERSNEVNGFFSCYLLASACPRYKGHTYIGF 60
           MR+ KEK E+ KTMAV EE+KEDDE   +EER NE N FFSCYLLASACPRYKGHTYIGF
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDE---DEERGNEGNRFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPSESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHP+ESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSG+ANK+KLAYTMLTLPAWCSLNITVNYFSTKYM NAAGCPSLP HM+VQV
Sbjct: 121 SAAATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQV 180

Query: 181 SPIHELPCYSEGDQCVLENEEDLEYNRECEEICSFRVYGSMKEVPNEVPKKLMD-STSTD 240
           SPI ELPCYSEGD+CVLENE D EYNRE +E+C  +V+  M EVPNE+P+ LMD  T TD
Sbjct: 181 SPIDELPCYSEGDRCVLENENDWEYNREYKEVCDSQVHDLMTEVPNEIPQTLMDYQTGTD 240

Query: 241 GRPHELRGCDKEFVNNERVPPSLCTPSDIDAGMSHELCGCDKEFENNERVPCSSCTPSYI 300
           GRPHELR                               G D+E E+NE+VP SSCTPSYI
Sbjct: 241 GRPHELR-------------------------------GSDEELEDNEQVPHSSCTPSYI 300

Query: 301 DAGMSYDLRGCDEELEEDEREPASCAQSCIVAAGTSRTEIIIDDGDEDQLEGNGMNLQKQ 360
           D  +SYDLRGCDE+      EPA CAQ CIV     RTEIIIDDG+E+ LEG+G  LQ+Q
Sbjct: 301 DVSVSYDLRGCDEQ------EPALCAQPCIV---EGRTEIIIDDGEENHLEGSGTKLQQQ 360

Query: 361 LGRKNLTSGIASEINKVSRCTNELPPVEYEIIDVSTPSPDCRTSSHRFKRRVTSE----- 420
            GRKNL SGIA+EI++VS     LPPVE E+IDVSTPSPDCRTSS++FKRRV ++     
Sbjct: 361 PGRKNL-SGIATEISEVS---YGLPPVENEVIDVSTPSPDCRTSSYKFKRRVITDSIFNP 385

Query: 421 --GNVGSSLVDQ 425
             GNVGS+LVDQ
Sbjct: 421 FRGNVGSNLVDQ 385

BLAST of HG10021767 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-66
Identity = 121/175 (69.14%), Postives = 139/175 (79.43%), Query Frame = 0

Query: 38  GFFSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGF 97
           GFF+CYLL S  PR+KG TYIGFTVNP+RRIRQHNGEI  GAWRTK+KRPWEMVLCIYGF
Sbjct: 26  GFFACYLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGF 85

Query: 98  PTNVSALQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNY 157
           PTNVSALQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPAW SLN+TVNY
Sbjct: 86  PTNVSALQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPAWNSLNLTVNY 145

Query: 158 FSTKYMKNAAGCPSLPEHMKVQVSPIHELPCYSE-GDQCVLENEEDLEYNRECEE 212
           FS+KY  +    PSLP HMKVQV  + +L  +++  D    E+EE  E N E ++
Sbjct: 146 FSSKYAHHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVNEEDDD 200

BLAST of HG10021767 vs. TAIR 10
Match: AT2G30350.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 126.7 bits (317), Expect = 4.9e-29
Identity = 65/109 (59.63%), Postives = 79/109 (72.48%), Query Frame = 0

Query: 104 LQFEWAWQHPSESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYM 163
           LQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPAW SLN+TVNYFS+KY 
Sbjct: 58  LQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPAWNSLNLTVNYFSSKYA 117

Query: 164 KNAAGCPSLPEHMKVQVSPIHELPCYSE-GDQCVLENEEDLEYNRECEE 212
            +    PSLP HMKVQV  + +L  +++  D    E+EE  E N E ++
Sbjct: 118 HHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVNEEDDD 166

BLAST of HG10021767 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 55.8 bits (133), Expect = 1.1e-07
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 40  FSCYLLASACPRYKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPT 99
           +S YL+ S     K  TY+G T +  RR++QHNGEIR GA  +   RPW     I GF  
Sbjct: 54  WSVYLILSTTEPIK--TYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTC 113

Query: 100 NVSALQFEWAWQ 112
              A  FE  W+
Sbjct: 114 LSQASSFESKWK 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894793.11.4e-19082.37structure-specific endonuclease subunit SLX1 [Benincasa hispida][more]
XP_008457313.12.5e-17679.14PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo][more]
KAA0060004.19.9e-17379.75Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ9726... [more]
XP_004153269.16.4e-17278.07structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus][more]
XP_022970648.11.7e-16471.06uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970649... [more]
Match NameE-valueIdentityDescription
Q8BX321.3e-2939.33Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b P... [more]
Q5PQP56.3e-2938.76Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Sl... [more]
Q32PI09.1e-2841.38Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2... [more]
Q0IH864.5e-2739.61Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a ... [more]
A7STV98.5e-2640.94Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis O... [more]
Match NameE-valueIdentityDescription
A0A1S3C5741.2e-17679.14Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3BBR04.8e-17379.75Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa... [more]
A0A0A0M0Y63.1e-17278.07Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 ... [more]
A0A6J1I6818.2e-16571.06Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
A0A6J1HKB92.5e-16170.60Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
AT2G30350.21.3e-6669.14Excinuclease ABC, C subunit, N-terminal [more]
AT2G30350.14.9e-2959.63Excinuclease ABC, C subunit, N-terminal [more]
AT5G43210.11.1e-0741.67Excinuclease ABC, C subunit, N-terminal [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 7..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..33
NoneNo IPR availablePANTHERPTHR20208:SF10STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 21..371
NoneNo IPR availablePANTHERPTHR20208STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 21..371
NoneNo IPR availableCDDcd10455GIY-YIG_SLX1coord: 39..114
e-value: 5.65647E-54
score: 173.573
IPR035901GIY-YIG endonuclease superfamilyGENE3D3.40.1440.10coord: 37..138
e-value: 2.4E-24
score: 87.2
IPR000305GIY-YIG endonucleasePFAMPF01541GIY-YIGcoord: 42..116
e-value: 1.8E-12
score: 47.3
IPR000305GIY-YIG endonucleasePROSITEPS50164GIY_YIGcoord: 38..120
score: 17.406075
IPR027520Structure-specific endonuclease subunit Slx1HAMAPMF_03100Endonuc_su_Slx1coord: 35..420
score: 23.770313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10021767.1HG10021767.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0051569 regulation of histone H3-K4 methylation
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006281 DNA repair
cellular_component GO:0016593 Cdc73/Paf1 complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0033557 Slx1-Slx4 complex
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0000993 RNA polymerase II complex binding