HG10021654 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10021654
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRhodanese domain-containing protein
LocationChr05: 13222829 .. 13227429 (-)
RNA-Seq ExpressionHG10021654
SyntenyHG10021654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAACAATGAAGGCGATGACCACCAATATGGCGTCCTTTTGTACTACAAGTACGCTCACATACCCAACCTCGACGACCTCGTCTCCTTCTACCAGTACAATTGCACCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCTCAGGGCGTCAATGTCACTGTAGCTTCTCTTAACTACCTATTTCGTTCCCCTTTCTCTATTTGGTTTCTAAGAAAGACTTTTAAGATGATTTTTCTGGTAGTTCCAGCAGTAGAATCAGCTGGAGTAATTGTTTACATGGTGTTTTCATTTTTTTCACCCGATTTGCTCATCTCTATGCTCTGTGATGATTCTGCATTTTTCAATTTTCTGCGGTGTTTTGAACTTTTCCTCTTTCTCCATTAGGTTTGTGGGAAGTTGTGCTCGTTGAAGAGTCATATTGCTGTAATGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATTGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGTAAAAGAGGATCCTCCCTTTCAATTTTTAACCCCAAAGTTTCAGCAATTTTAATGTTAAAGCTTGCACATTTTTCAGGAGTTGGTCACCCTTTGTTCAAATCCTCTATCGAAGTTACCTGAAATTACAGAAGCAGGAACCCATCTCTCTGCAGCTGAATTTCATTCTGTTCTTGAGAGTGCTGGTTAGACTATTCTCTTCTTCTTCTTATGCTTTATTTGTTACTTTACTGACTTATAACAACAGGGGATGACTTTATGCAGAACCGGGAATGGAATGATTCGAAATAAAGTTCTTTTGACTAGTAATCACATACTTTGCAGGGCTACTTTCGGATGAAGAGACACTGGAACATAGTAGAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAATCACCAAACGTTGAAACACTGGATCCAGAAATTAGGCAATATAGTGATTTGCCATCTTGGATTGATGATAATTCTGAAAAGTTGCGCGGAAAACACATTCTTATGTTAGTTGTCTCAAACTATACGTTATTCGATCTGGATGCCTTTTTCTTATTGTTCATGACATGGCTCAAAAAGTAGTGGCACCAATTGCTTATGTTTGGCATGCGGCTTACAGTGGTTTAATCTATTGATTCCTTTGGATGACGAGTACTTTGGGCATTTAGTTTAACACTCGTGTTCCTTTGTCTGCATATTTGGATGTAAGTGTGTTTGTGGAGCTAAAAGTGCTTGATTTTTATTTTCAAGTTCAGAACTACCATTTCCAGCTGCATATAATCAAATTTGGTTATTGTATATATTTGCAAATTCTAATTCATAGGTAGTCTTACACGTGAATAGCTAAGATTGCCCTTGTACAAAATAATGTCTTCCTTGTTGCCAGCCTCTTTTCCTCAATGCTTGTATTAACAGTACTGGATTTGTTCAATAGCTCAAAAGGCAGTTTTTTAGAACAATACATCATGACTCAAGTTTCATCTTCTTCCTATCTAATAGGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCCAAAGGATCTGGATTTCAGAATGTCTTTCAGGTGCTATCATATGAAATATTTCTGAATTTTATATCTTACACATATATATTGACGTTATTTATAATGTATCTTGGATTTTTGGGCATATTTTCATTTTCCCCAATTTCGAACTTATGGATTGTTAACTAATGAATGATAGGCTAAAATATGGTTTTTTAATTTTATTTTTTATTTTTTATCTAGTCTCTAAAGATTTAGGCCATGCCTTGGTGAGTTCATGGTTCATGCATTATGGCTTTAGTTGGTGTTAGTTTTATCGCATCAGAGTCACTTTATATGTTGGTGATGGGGTGCTTTTCGCTGCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTCCTGATGGCGGATTTTTTGCAGGAAAGAATTTTGTGTTTGATCACAGGTGAAAGCTCTAATGCCCTCACATCACCATTGGTACTAATTAGGATGTGATAGTTTAGACAAGATTCTGAAGAGGGAGACATCAAACCTGATATATATTCATTTAAGTATTGAGCCAAACAATTTTGTATGAACATAATTTGGCTCCTCAAGTAAATTTCCAACTTTTGAAATCCTCAAATATTCTAAGAGGAATTGGTGACAGCATCTTCATGGGAAACTTGTGGAAATAGATGATGTTGCAATTTGAGCTGAAATTTTCTAATATTCAGTTCTGTGGTTATAGGATATCAGTTGGGAGCTCAACTCAAGATATTATAGGGACCTGCCTTATTTGTGGAAACCCATCTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTGCTGGTGTGTGCTAATTGCCAGGTCTGCTATGATAGTAATCTCTTTGTACTCATTCTTATCTGGCTTGTTGTTCCATTATTTCGTGATTTTTTAGTTAACAACATGAATTCTAAAATTTGTTTTCACCATTCCGTTATTATATCATTTTTTATCACCTTTAGCTTTCTTGTGATTTGTGGTATCCTGCTTCTGCATCCTGTTATTGCGGCTCAGAAAAAGGAGACCCTATATGTTTGTGAGCTTTGTCGGAAGCATGGCAAGGAGGATGAGGTGATTTCACAATTCCAGAGCTCAAATTCTGAAGCAGTCTTGCTGCAAAGTGAACTTGAAATTGGTTCCCAGCAACTTCCGTGGAGTAACGGTAAGGTCTGTGTTAATTCTTCTATTACTTCTTGCATGTCAAGGAATATATGGATCTAATATTTTATGATATGATTTGAATTATCGTTATATTGTTCAGTTAATACCATCACAAGATTCAACTCTTTGATTTAGAGTTTGTATGAGAATCCTTCTAGAATAACTGTTTATAGCAAAAGTATTTTGATCCAATGAGAAGCTTATCAACAAATAATTTTGACGTTCAGTTTTACACTCTTAAAAGTGCTTTTGAGATCTCTCTCATTCAAGCACTTCAAAAACATTTTGCCAGTGGTCCCTTTGGGGACATCCGATCAAACATTTTGCCAGTGCCTTCATAAAGCACTTCAAACGTTTTTGACTTTTTACTAAAGCACTTTTACGTTACTCCTAGACAATAGACATGATGGTTTTGAGGGCCAAAATATGTGATTATTACGGTCTTTCCACACTAAGCTCAACCAGAATCTGTTAGCTCTTTATGAGCAATAAATTGTTTTACTATAGAATCCTCAATGTGGTCATGTTTTCTATCAAATCATATTTCTGATGTTCAGCAATCTAATATGGATTTTAAAGTACTGCCTTGCCTTTCTATTTTGAACCTAAGAGTTGGAAGTCAAATCTTTCTTCTTCCTTTAATTGATCTGTAATTCAAACTCTTATTCAGGTTCTAGGACACCAAGAAGATTGAGAGTCCTGTGTTTGCATGGATTTAGACAGAATGCTTCGAGCTTTAAAGGAAGAACTGCATCATTAGCAAAAAAACTCAAAACCATGGTGGAGTTTGTGTATGTTGATGCACCTCACGAGTTATCGTTCATTTACCATCCTCGGGACAGCGAGCCTCGGGAAACTTGTGTGACATCTTCAGTACAGCCAAATTGTCCTCCGCCACTGGAGAGTTGCAAGAAGAAGTTTGCTTGGTTAATATCACACAATGCAGGTGAAAGGACTGAAACTAAATGGGAAGTTGCAGATGCCCCATTTGATCCACTCCAATACCAGAAACAAACTGATGGATTTGAAGAATCATTGGAATATTTGAAGACCGTATTTTCCGAGAAAGGACCATTTGATGGGATCTTAGGTTTTTCACAAGGAGCAGCAATGGCTGCCGCAGTTTGTTCGAGAAAAACGAGTCTAAAGGGTGCAATAGACTTCCGATTTGCAGTGTTGTGTTCTGGGTTTCCTCTTCAAATGCCTGAATTGGACCATGCACTGATCAACTGCCCCACACTTCACATATTTGGTAGTGATCGGGGAAATGATAGGCAGATTGCTAACAAAACGAGCAGAGACCTTGCTTCTTGTTTTGATGCAAGTTGTTCTGTGATTGTCGAACATGACTCTGGTCACATAATTCCTACCCGACCTCCCTACATTGACGAGATAAAAGAGTTCCTTCAACGTTTTATTTGATTGGATTCATGCGTGTGACCTACAACCATTGAAGTTGCTACGAGAGCTTGATCATACAAGGGATTATCTTTGATTTCTCGACGAAAAGGTCGGTTCACTCTTGGATTGTAATGGCACTTTTTTGCTTTATAGTTTTAGCTAATTGTAGACTTCTTTTTTACTAACTTGTTAAATTTCTAGTTTACCTGAAAGTTTTTTACATCAAAAGGATGAAGGATTTACAGAGCTTGGTGTTATAATTGATCTATAAGCTAGCAAAATTCTGATTATTTAACTATAAGTTACATAATTTCCTTTTGTAATTTCACTATTTCCAGGGTAGATGCTGATTTTATGGGTTATGCTATAGTTTTTGTTCTAAATTTTCATGATTATGCCCATTAG

mRNA sequence

ATGGCGAACAATGAAGGCGATGACCACCAATATGGCGTCCTTTTGTACTACAAGTACGCTCACATACCCAACCTCGACGACCTCGTCTCCTTCTACCAGTACAATTGCACCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCTCAGGGCGTCAATGTCACTGTAGCTTCTCTTAACTACCTATTTCGTTCCCCTTTCTCTATTTGGTTTCTAAGAAAGACTTTTAAGATGATTTTTCTGGTTTGTGGGAAGTTGTGCTCGTTGAAGAGTCATATTGCTGTAATGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATTGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGAGTTGGTCACCCTTTGTTCAAATCCTCTATCGAAGTTACCTGAAATTACAGAAGCAGGAACCCATCTCTCTGCAGCTGAATTTCATTCTGTTCTTGAGAGTGCTGGGCTACTTTCGGATGAAGAGACACTGGAACATAGTAGAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAATCACCAAACGTTGAAACACTGGATCCAGAAATTAGGCAATATAGTGATTTGCCATCTTGGATTGATGATAATTCTGAAAAGTTGCGCGGAAAACACATTCTTATGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCCAAAGGATCTGGATTTCAGAATGTCTTTCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTCCTGATGGCGGATTTTTTGCAGGAAAGAATTTTGTGTTTGATCACAGGATATCAGTTGGGAGCTCAACTCAAGATATTATAGGGACCTGCCTTATTTGTGGAAACCCATCTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTGCTGGTGTGTGCTAATTGCCAGAAAAAGGAGACCCTATATGTTTGTGAGCTTTGTCGGAAGCATGGCAAGGAGGATGAGGTGATTTCACAATTCCAGAGCTCAAATTCTGAAGCAGTCTTGCTGCAAAGTGAACTTGAAATTGGTTCCCAGCAACTTCCGTGGAGTAACGGTTCTAGGACACCAAGAAGATTGAGAGTCCTGTGTTTGCATGGATTTAGACAGAATGCTTCGAGCTTTAAAGGAAGAACTGCATCATTAGCAAAAAAACTCAAAACCATGGTGGAGTTTGTGTATGTTGATGCACCTCACGAGTTATCGTTCATTTACCATCCTCGGGACAGCGAGCCTCGGGAAACTTGTGTGACATCTTCAGTACAGCCAAATTGTCCTCCGCCACTGGAGAGTTGCAAGAAGAAGTTTGCTTGGTTAATATCACACAATGCAGGTGAAAGGACTGAAACTAAATGGGAAGTTGCAGATGCCCCATTTGATCCACTCCAATACCAGAAACAAACTGATGGATTTGAAGAATCATTGGAATATTTGAAGACCGTATTTTCCGAGAAAGGACCATTTGATGGGATCTTAGGTTTTTCACAAGGAGCAGCAATGGCTGCCGCAGTTTGTTCGAGAAAAACGAGTCTAAAGGGTGCAATAGACTTCCGATTTGCAGTGTTGTGTTCTGGGGTAGATGCTGATTTTATGGGTTATGCTATAGTTTTTGTTCTAAATTTTCATGATTATGCCCATTAG

Coding sequence (CDS)

ATGGCGAACAATGAAGGCGATGACCACCAATATGGCGTCCTTTTGTACTACAAGTACGCTCACATACCCAACCTCGACGACCTCGTCTCCTTCTACCAGTACAATTGCACCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCTCAGGGCGTCAATGTCACTGTAGCTTCTCTTAACTACCTATTTCGTTCCCCTTTCTCTATTTGGTTTCTAAGAAAGACTTTTAAGATGATTTTTCTGGTTTGTGGGAAGTTGTGCTCGTTGAAGAGTCATATTGCTGTAATGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATTGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGAGTTGGTCACCCTTTGTTCAAATCCTCTATCGAAGTTACCTGAAATTACAGAAGCAGGAACCCATCTCTCTGCAGCTGAATTTCATTCTGTTCTTGAGAGTGCTGGGCTACTTTCGGATGAAGAGACACTGGAACATAGTAGAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAATCACCAAACGTTGAAACACTGGATCCAGAAATTAGGCAATATAGTGATTTGCCATCTTGGATTGATGATAATTCTGAAAAGTTGCGCGGAAAACACATTCTTATGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCCAAAGGATCTGGATTTCAGAATGTCTTTCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTCCTGATGGCGGATTTTTTGCAGGAAAGAATTTTGTGTTTGATCACAGGATATCAGTTGGGAGCTCAACTCAAGATATTATAGGGACCTGCCTTATTTGTGGAAACCCATCTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTGCTGGTGTGTGCTAATTGCCAGAAAAAGGAGACCCTATATGTTTGTGAGCTTTGTCGGAAGCATGGCAAGGAGGATGAGGTGATTTCACAATTCCAGAGCTCAAATTCTGAAGCAGTCTTGCTGCAAAGTGAACTTGAAATTGGTTCCCAGCAACTTCCGTGGAGTAACGGTTCTAGGACACCAAGAAGATTGAGAGTCCTGTGTTTGCATGGATTTAGACAGAATGCTTCGAGCTTTAAAGGAAGAACTGCATCATTAGCAAAAAAACTCAAAACCATGGTGGAGTTTGTGTATGTTGATGCACCTCACGAGTTATCGTTCATTTACCATCCTCGGGACAGCGAGCCTCGGGAAACTTGTGTGACATCTTCAGTACAGCCAAATTGTCCTCCGCCACTGGAGAGTTGCAAGAAGAAGTTTGCTTGGTTAATATCACACAATGCAGGTGAAAGGACTGAAACTAAATGGGAAGTTGCAGATGCCCCATTTGATCCACTCCAATACCAGAAACAAACTGATGGATTTGAAGAATCATTGGAATATTTGAAGACCGTATTTTCCGAGAAAGGACCATTTGATGGGATCTTAGGTTTTTCACAAGGAGCAGCAATGGCTGCCGCAGTTTGTTCGAGAAAAACGAGTCTAAAGGGTGCAATAGACTTCCGATTTGCAGTGTTGTGTTCTGGGGTAGATGCTGATTTTATGGGTTATGCTATAGTTTTTGTTCTAAATTTTCATGATTATGCCCATTAG

Protein sequence

MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQFQSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLKTMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGERTETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSRKTSLKGAIDFRFAVLCSGVDADFMGYAIVFVLNFHDYAH
Homology
BLAST of HG10021654 vs. NCBI nr
Match: XP_038895686.1 (rhodanese-like domain-containing protein 6 [Benincasa hispida])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.2e-294
Identity = 508/559 (90.88%), Postives = 518/559 (92.67%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MANNE DDHQYGVLLYYKYAHIP+LD LVSFYQ NCTSLALVGRVRLASQGVNVT     
Sbjct: 1   MANNEDDDHQYGVLLYYKYAHIPDLDHLVSFYQSNCTSLALVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSLKSHIA MKLNSLFDGTDFKLA CHQPLNDIV
Sbjct: 61  ---------------------VCGKLCSLKSHIAAMKLNSLFDGTDFKLASCHQPLNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPLSKLPEIT+AGTHLSA EFHSVLESAGLLSDEETLE
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITQAGTHLSATEFHSVLESAGLLSDEETLE 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           +SRDVVLLDARNLYETRVGKFQ+PNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT
Sbjct: 181 NSRDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ
Sbjct: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICGNP DDYSSRCRCKYCRMLVLVC NCQ+KETL+VCELCRKHGKE EVISQF
Sbjct: 301 DIIGTCLICGNPFDDYSSRCRCKYCRMLVLVCDNCQEKETLFVCELCRKHGKEGEVISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QSS S+ VLLQSELEI SQQLPWSNG R+PRRLRVLCLHGFRQNASSFKGRTASLAKKLK
Sbjct: 361 QSSKSKVVLLQSELEISSQQLPWSNGCRSPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
           TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER
Sbjct: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480

Query: 481 T-ETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCS 540
           + ETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMA AVCS
Sbjct: 481 SEETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAGAVCS 533

Query: 541 RKTSLKGAIDFRFAVLCSG 559
           RKTSLKGA+DFRFAVLCSG
Sbjct: 541 RKTSLKGAVDFRFAVLCSG 533

BLAST of HG10021654 vs. NCBI nr
Match: XP_011654855.1 (rhodanese-like domain-containing protein 6 [Cucumis sativus] >KGN65012.1 hypothetical protein Csa_022714 [Cucumis sativus])

HSP 1 Score: 993.4 bits (2567), Expect = 8.1e-286
Identity = 494/558 (88.53%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MA NE DDHQYGVLLYYKYA+IPNLDDLVSFYQ NCTSLALVGRVRLASQGVNVT     
Sbjct: 1   MAKNEDDDHQYGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKL SLKSHIA MKLNSLFDGTDFKLA CHQP NDIV
Sbjct: 61  ---------------------VCGKLSSLKSHIAAMKLNSLFDGTDFKLASCHQPSNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPLSKLPEI+EAGTHLSAAEFHSVLE+AGLLSDEET E
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEISEAGTHLSAAEFHSVLETAGLLSDEETPE 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNV TLDPEIRQYSDLPSWIDDNSEKL+GK ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVVTLDPEIRQYSDLPSWIDDNSEKLQGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRI+VGSSTQ
Sbjct: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTQ 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICGNP DDYSSRCRCKYCRMLVLVC NCQKKETLYVCELCRKHGKE EVISQF
Sbjct: 301 DIIGTCLICGNPFDDYSSRCRCKYCRMLVLVCDNCQKKETLYVCELCRKHGKEGEVISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QSS SE VLLQSELE GSQQLPWSNG R+PR+LRVLCLHGFRQNASSFKGRTASLAKKLK
Sbjct: 361 QSSKSEVVLLQSELENGSQQLPWSNGCRSPRKLRVLCLHGFRQNASSFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
           TMVEFVYVDAPHELSFIYHPRDSEP ETCVTS VQPN PPPLESCKKKFAWL+S+NAGER
Sbjct: 421 TMVEFVYVDAPHELSFIYHPRDSEPWETCVTSLVQPNHPPPLESCKKKFAWLVSNNAGER 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
           TET WEVADAPFDPLQYQKQTDGFE+SL YLKTVFSEKGPFDGILGFSQGAAMAAAVCSR
Sbjct: 481 TETNWEVADAPFDPLQYQKQTDGFEKSLAYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 532

Query: 541 KTSLKGAIDFRFAVLCSG 559
           K SLKGA++FRFAVLCSG
Sbjct: 541 KLSLKGAVEFRFAVLCSG 532

BLAST of HG10021654 vs. NCBI nr
Match: XP_008464312.1 (PREDICTED: rhodanese-like domain-containing protein 6 [Cucumis melo])

HSP 1 Score: 963.8 bits (2490), Expect = 6.8e-277
Identity = 483/558 (86.56%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MANNE DDH  GVLLYYKYA+IPNLDDLVSFYQ NCTSLALVGRVRLASQGVNVT     
Sbjct: 1   MANNEDDDH--GVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKL SLKSHIAVMKLNSLFDGTDFKLA CHQP NDIV
Sbjct: 61  ---------------------VCGKLSSLKSHIAVMKLNSLFDGTDFKLASCHQPSNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPLSKLPEI+EAGTHLSAAEFHSVLESAGLLSDEET E
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEISEAGTHLSAAEFHSVLESAGLLSDEETPE 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNVETLDPEIRQYSDLPSWIDDNSEKLRGK ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRI+VGSST 
Sbjct: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTP 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICGNP DDYSSRCRCKYCRMLVLVC NCQ+KETLYVCELCRKHGKE EVISQF
Sbjct: 301 DIIGTCLICGNPFDDYSSRCRCKYCRMLVLVCDNCQEKETLYVCELCRKHGKEGEVISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QSS SE VLLQSEL      +PWSNG R+PR+LRVLCLHGFRQNAS FKGRTASLAKKLK
Sbjct: 361 QSSKSEVVLLQSEL------VPWSNGCRSPRKLRVLCLHGFRQNASGFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
           TMVEFVYVDAPHELSFIYHPRDS+P ETCVTSSVQP+ PPPLESCKKKFAWL+S NAG+R
Sbjct: 421 TMVEFVYVDAPHELSFIYHPRDSDPWETCVTSSVQPHRPPPLESCKKKFAWLVSDNAGKR 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
           TET WEVADAPFDPLQYQKQT GFE+SL YLKTVFSEKGPFDGILGFSQGAAM AAVCS+
Sbjct: 481 TETNWEVADAPFDPLQYQKQTTGFEKSLAYLKTVFSEKGPFDGILGFSQGAAMVAAVCSK 524

Query: 541 KTSLKGAIDFRFAVLCSG 559
           K SLKGA++FRFAVLCSG
Sbjct: 541 KPSLKGAVEFRFAVLCSG 524

BLAST of HG10021654 vs. NCBI nr
Match: XP_022159722.1 (rhodanese-like domain-containing protein 6 [Momordica charantia])

HSP 1 Score: 953.4 bits (2463), Expect = 9.2e-274
Identity = 471/558 (84.41%), Postives = 498/558 (89.25%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           M NNE DD QYGVLLYYKYA IP+LDDLVSFY+ NC+SL LVGRVRLASQGVNVT     
Sbjct: 1   MVNNE-DDDQYGVLLYYKYAQIPDLDDLVSFYESNCSSLGLVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSL+SHI  MKLNSLFDGTDFKLA CHQPLNDIV
Sbjct: 61  ---------------------VCGKLCSLRSHITAMKLNSLFDGTDFKLASCHQPLNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPL KLPEITEAGTHLSAAEFHSVLESAGLLSDEET  
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLLKLPEITEAGTHLSAAEFHSVLESAGLLSDEETPR 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNVETLDPEIRQYSDLPSWIDDNSEKLR K ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRAKKILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYI+SKG  FQNVFQLYGGIQRYLEQFPDGG FAGKNFVFDHR+SVGSST+
Sbjct: 241 GGIRCEMASAYIRSKGPDFQNVFQLYGGIQRYLEQFPDGGLFAGKNFVFDHRMSVGSSTE 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICG+P DDYSSRCRCK CRMLVLVC NCQKKETLYVCELC+KHGK+  +ISQF
Sbjct: 301 DIIGTCLICGDPFDDYSSRCRCKNCRMLVLVCDNCQKKETLYVCELCQKHGKDGGLISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QS+ SE V LQ+EL+IGSQQLPWSNG R+PR+LR+LCLHGFRQNASSFKGRTASLAKKLK
Sbjct: 361 QSTKSEVVSLQNELQIGSQQLPWSNGCRSPRKLRILCLHGFRQNASSFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
            MVEFVYVDAPHELSFIYHPRDSEP+E C+TSSVQPN PPPL  C+KKFAWLI+HNAGER
Sbjct: 421 IMVEFVYVDAPHELSFIYHPRDSEPQEACMTSSVQPNRPPPLGCCRKKFAWLIAHNAGER 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
            ET+WEVADAPFDPLQYQKQTDGFEESL YLKT+FSE+GPFDGILGFSQGAAM AAVCSR
Sbjct: 481 NETQWEVADAPFDPLQYQKQTDGFEESLAYLKTLFSEQGPFDGILGFSQGAAMVAAVCSR 531

Query: 541 KTSLKGAIDFRFAVLCSG 559
           KTSLKGAIDFRFAVLCSG
Sbjct: 541 KTSLKGAIDFRFAVLCSG 531

BLAST of HG10021654 vs. NCBI nr
Match: XP_022931639.1 (rhodanese-like domain-containing protein 6 isoform X1 [Cucurbita moschata])

HSP 1 Score: 920.2 bits (2377), Expect = 8.7e-264
Identity = 464/561 (82.71%), Postives = 488/561 (86.99%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MAN E DDHQ+GVLLYYKYA IP+LDDLVSFY+ NC SL LVGRVRLASQGVNVT     
Sbjct: 1   MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSLKSHIAVM LNSLFDGTDFKLA CHQP+ND+V
Sbjct: 61  ---------------------VCGKLCSLKSHIAVMNLNSLFDGTDFKLASCHQPMNDVV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTL SNPL KLPEITEAGTHLSAAEFHSVLE AGLLSDE+   
Sbjct: 121 ARECGFTSLSVRVVQELVTLRSNPLLKLPEITEAGTHLSAAEFHSVLERAGLLSDEKKRI 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           HS+DVVLLDARNLYETRVGKF++PN+ETLDPEIRQYSDL  WIDDNSEKLRGK ILMYCT
Sbjct: 181 HSKDVVLLDARNLYETRVGKFKTPNIETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGS FQNVFQLYGGIQRYLEQFP GGFF+GKNFVFDHRISVGSST+
Sbjct: 241 GGIRCEMASAYIKSKGSEFQNVFQLYGGIQRYLEQFPYGGFFSGKNFVFDHRISVGSSTE 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCL CG+P DDYSSRCRCKYCRMLVLVC NCQKK TLYVCELCRK+GK+   ISQF
Sbjct: 301 DIIGTCLSCGDPFDDYSSRCRCKYCRMLVLVCDNCQKKNTLYVCELCRKNGKDGGPISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGS--RTPRRLRVLCLHGFRQNASSFKGRTASLAKK 420
           QS+  E V  QS+ EIGSQQLPWSNG   R+PR+LRVLCLHGFRQNASSFKGRTASLAKK
Sbjct: 361 QSTKLEIVSQQSDHEIGSQQLPWSNGKVCRSPRKLRVLCLHGFRQNASSFKGRTASLAKK 420

Query: 421 LKTMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISH-NA 480
           LKTMVEFVYVDAPHELSFIYHPRD   RETCVTSSVQPNCPPP+E CKKKFAWLI+  N 
Sbjct: 421 LKTMVEFVYVDAPHELSFIYHPRD---RETCVTSSVQPNCPPPVEHCKKKFAWLIAEDNT 480

Query: 481 GERTETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAV 540
           GER+ETKWEVADA FDPLQYQKQTDGFEESL YLKTVFSEKGPFDGILGFSQGAAM AAV
Sbjct: 481 GERSETKWEVADALFDPLQYQKQTDGFEESLAYLKTVFSEKGPFDGILGFSQGAAMTAAV 532

Query: 541 CSRKTSLKGAIDFRFAVLCSG 559
           CSRK S+KGA+D RFAVLCSG
Sbjct: 541 CSRKMSVKGAVDVRFAVLCSG 532

BLAST of HG10021654 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 7.0e-192
Identity = 342/552 (61.96%), Postives = 397/552 (71.92%), Query Frame = 0

Query: 7   DDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSP 66
           D+ QYGVLLYYKY  +P+LD+LVSFY+ +C SL L+GRVRL+ +GVNVTV          
Sbjct: 16  DEEQYGVLLYYKYTSVPDLDELVSFYESSCNSLGLLGRVRLSPKGVNVTVG--------- 75

Query: 67  FSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGF 126
                            GKL +L+ HIA  K N LF+GTDFKLA CH PLND VA ECGF
Sbjct: 76  -----------------GKLTALEEHIAAAKSNCLFEGTDFKLASCHHPLNDKVAEECGF 135

Query: 127 TSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVV 186
           TSLS+RVV+ELVT    P  K PEI+ AG HLSAAEFHSVL+SA   S+      ++++V
Sbjct: 136 TSLSIRVVEELVTFSPCPPLKPPEISNAGKHLSAAEFHSVLQSANGKSE------NKELV 195

Query: 187 LLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCE 246
           LLDARNLYETR+GKF+S NVETLDPEIRQYSDLP+WID N+EK++GK++LMYCTGGIRCE
Sbjct: 196 LLDARNLYETRIGKFESENVETLDPEIRQYSDLPTWIDQNAEKMKGKNVLMYCTGGIRCE 255

Query: 247 MASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTC 306
           MASAYI+SKG+GF+N FQLYGGIQRYLEQFP GGFF GKNFVFDHRISVGSS +DIIG+C
Sbjct: 256 MASAYIRSKGAGFENTFQLYGGIQRYLEQFPSGGFFKGKNFVFDHRISVGSSKEDIIGSC 315

Query: 307 LICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQFQSSNSE 366
           L+C N  DDYS RCRC+ CRMLVLVC +C+ K  +Y+CELCRKHGK             E
Sbjct: 316 LLCNNTFDDYSPRCRCRLCRMLVLVCNHCRVKGDIYICELCRKHGK------------GE 375

Query: 367 AVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLKTMVEFV 426
             L    L   S+    SNG  T R+LR+LCLHGFRQNASSFKGRT SLAKKLK + E V
Sbjct: 376 VPLSLDPLNQPSE----SNGDNTRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELV 435

Query: 427 YVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGERTETKWE 486
           ++DAPHEL FIY                     PP   C KKFAWL+S +  + +ET W 
Sbjct: 436 FIDAPHELQFIYQ-----------------TATPPSGVCNKKFAWLVSSDFDKPSETGWT 495

Query: 487 VADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSRKTSLKG 546
           VA   FDPLQYQ QT+GF++SL YLKT F EKGPFDGILGFSQGAAMAAAVC ++  L G
Sbjct: 496 VAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFSQGAAMAAAVCGKQEQLVG 502

Query: 547 AIDFRFAVLCSG 559
            IDFRF VLCSG
Sbjct: 556 EIDFRFCVLCSG 502

BLAST of HG10021654 vs. ExPASy Swiss-Prot
Match: Q5T7W7 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TSTD2 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.0e-45
Identity = 126/354 (35.59%), Postives = 174/354 (49.15%), Query Frame = 0

Query: 13  VLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPFSIWFL 72
           VLLYY Y  + +   + ++    C  L L G++R+A++G+N TV                
Sbjct: 170 VLLYYCYHDLEDPQWICAWQTALCQHLHLTGKIRIAAEGINGTVG--------------- 229

Query: 73  RKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGFTSLSVR 132
                      G   + + ++ VM    L     FK  LC             F  L V 
Sbjct: 230 -----------GSKLATRLYVEVMLSFPL-----FKDDLCKDDFKTSKGGAHCFPELRVG 289

Query: 133 VVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVLLDARN 192
           V +E+V +  +P  K     + G HLS  EFH  +E     +++E      D +LLD RN
Sbjct: 290 VFEEIVPMGISP--KKISYKKPGIHLSPGEFHKEVEKFLSQANQE----QSDTILLDCRN 349

Query: 193 LYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEMASAYI 252
            YE+++G+FQ      L P+IR++S  PS++D N E  R K +LMYCTGGIRCE  SAY+
Sbjct: 350 FYESKIGRFQG----CLAPDIRKFSYFPSYVDKNLELFREKRVLMYCTGGIRCERGSAYL 409

Query: 253 KSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTCLICGNP 312
           K+KG   + VFQL GGI +YLE+FPD GF+ GK FVFD R ++ S   D++  C  CG  
Sbjct: 410 KAKGV-CKEVFQLKGGIHKYLEEFPD-GFYKGKLFVFDERYAL-SYNSDVVSECSYCGAR 469

Query: 313 SDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQFQSSNSE 367
            D Y   C    CR LVL C  CQ +     C  C+  G   +V    Q S  E
Sbjct: 470 WDQY-KLCSTPQCRQLVLTCPACQGQGFTACCVTCQDKGSR-KVSGPMQDSFKE 477

BLAST of HG10021654 vs. ExPASy Swiss-Prot
Match: Q5RCP1 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Pongo abelii OX=9601 GN=TSTD2 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 4.5e-45
Identity = 125/354 (35.31%), Postives = 173/354 (48.87%), Query Frame = 0

Query: 13  VLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPFSIWFL 72
           VLLYY Y  + +   + ++    C  L L G++R+A++G+N TV                
Sbjct: 170 VLLYYCYRDLEDPQWICAWQTALCQQLHLTGKIRIAAEGINGTVG--------------- 229

Query: 73  RKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGFTSLSVR 132
                      G   + + ++ VM    L     FK  LC             F  L V 
Sbjct: 230 -----------GSKLATRLYVEVMLSFPL-----FKDDLCKDDFKTSKGGAHCFPELRVG 289

Query: 133 VVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVLLDARN 192
           V +E+V +  +P  K     + G HLS  EFH  +E     +++E      D +LLD RN
Sbjct: 290 VFEEIVPMGISP--KKISYKKPGIHLSPGEFHKEVEKFLSQANQE----QSDTILLDCRN 349

Query: 193 LYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEMASAYI 252
            YE+++G+FQ      L P+IR++S  PS++D N E  R K +LMYCTGGIRCE  SAY+
Sbjct: 350 FYESKIGRFQG----CLAPDIRKFSYFPSYVDKNLELFREKRVLMYCTGGIRCERGSAYL 409

Query: 253 KSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTCLICGNP 312
           K+KG   + VFQL GGI +YLE+FPD GF+ GK FVFD R ++ S   D++  C  CG  
Sbjct: 410 KAKGV-CKEVFQLKGGIHKYLEEFPD-GFYKGKLFVFDERYAL-SYNSDVVSECSYCGAR 469

Query: 313 SDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQFQSSNSE 367
            D Y   C    C  LVL C  CQ +     C  C+  G   +V    Q S  E
Sbjct: 470 WDQY-KLCSTPQCHQLVLTCPACQGQGFTACCVTCQDKGSR-KVSGPMQDSFKE 477

BLAST of HG10021654 vs. ExPASy Swiss-Prot
Match: Q3U269 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tstd2 PE=2 SV=3)

HSP 1 Score: 180.6 bits (457), Expect = 4.9e-44
Identity = 123/353 (34.84%), Postives = 178/353 (50.42%), Query Frame = 0

Query: 8   DHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPF 67
           D +  VLLYY Y  + +   + ++    C  L L G++R+A++G+N TV           
Sbjct: 164 DEEGEVLLYYCYCDLEDPHWVCAWQTALCHHLHLTGKIRIATEGINGTVG---------- 223

Query: 68  SIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGFT 127
                           G   + + ++ VM    L     FK  L              F 
Sbjct: 224 ----------------GSKVATRLYVEVMLSCPL-----FKDYLSEDDFKSSKGGSHCFP 283

Query: 128 SLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVL 187
            L V V +E+V +  +P        + G HLS  EFH  +E   LLS  ++ E   + ++
Sbjct: 284 ELRVGVFEEIVPMGISP--SQVSYKKPGIHLSPGEFHKEIEK--LLS--QSSEEQGNTII 343

Query: 188 LDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEM 247
           LD RN YE+++G+FQ      L P+IR++S  PS++D N +  R K +LMYCTGGIRCE 
Sbjct: 344 LDCRNFYESKIGRFQG----CLAPDIRKFSYFPSYVDKNLDIFRQKRVLMYCTGGIRCER 403

Query: 248 ASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTCL 307
            SAY+++KG   + VFQL GGI +YLE+FPD GF+ GK FVFD R ++  ++  ++  C 
Sbjct: 404 GSAYLRAKGV-CKEVFQLKGGIHKYLEEFPD-GFYKGKLFVFDERFALAYNS-SVVSECS 463

Query: 308 ICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCR-KHGKEDEVISQ 360
            CG P D Y   C    CR LVL C+ CQ +     C  C+ K GK+    SQ
Sbjct: 464 YCGAPWDQY-KLCSTPQCRQLVLTCSACQGQGFTACCVTCQDKGGKQASGPSQ 471

BLAST of HG10021654 vs. ExPASy Swiss-Prot
Match: Q65EW5 (tRNA uridine(34) hydroxylase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=trhO PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 4.3e-32
Identity = 115/378 (30.42%), Postives = 170/378 (44.97%), Query Frame = 0

Query: 9   HQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPFS 68
           +QY VLLYYKY HI N +     +   C  L L GR+ +A +G+N TV+           
Sbjct: 3   NQYRVLLYYKYVHIDNPEQFAEDHLKFCKDLGLKGRILVAGEGINGTVS----------- 62

Query: 69  IWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGFTS 128
                          G +     +++ MK +  F+   FK+           +    F  
Sbjct: 63  ---------------GTVEQTDRYMSEMKSDPRFEDMVFKID---------ESEGHAFKK 122

Query: 129 LSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVLL 188
           + VR   ELVTL         E+T  G +L   EF+  ++               D +++
Sbjct: 123 MHVRHRDELVTLRLEDDIDPNELT--GKYLEPKEFYEAMQ-------------QEDTIVV 182

Query: 189 DARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEMA 248
           DARN YE  +G F+      + P+I+ + +LP WI DN EKL GK IL YCTGGIRCE  
Sbjct: 183 DARNDYEYDLGHFRG----AIRPDIKAFRELPEWIRDNKEKLEGKKILTYCTGGIRCEKF 242

Query: 249 SAYIKSKGSGFQNVFQLYGGIQRY-LEQFPDGGFFAGKNFVFDHRISVGSSTQD--IIGT 308
           S ++K +  GF++V QL+GGI  Y  +    G  + GK +VFD RISV  + ++  I+G 
Sbjct: 243 SGWLKKE--GFEDVSQLHGGIVTYGKDPEVQGELWDGKCYVFDERISVPVNQKEHVIVGK 302

Query: 309 CLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVC-ELCRKHGK----------ED 368
               G P + Y + C    C   + +C+   +   L  C   CR H +          E+
Sbjct: 303 DYFTGEPCERYVN-CANPECNKQI-ICSEENEHRYLRGCTHECRVHPRNLYVKEHGLSEE 322

Query: 369 EVISQFQSSNSEAVLLQS 373
           EV  + +    E    QS
Sbjct: 363 EVQERLEKLKEEEHAAQS 322

BLAST of HG10021654 vs. ExPASy TrEMBL
Match: A0A0A0LW43 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G180760 PE=4 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.9e-286
Identity = 494/558 (88.53%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MA NE DDHQYGVLLYYKYA+IPNLDDLVSFYQ NCTSLALVGRVRLASQGVNVT     
Sbjct: 1   MAKNEDDDHQYGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKL SLKSHIA MKLNSLFDGTDFKLA CHQP NDIV
Sbjct: 61  ---------------------VCGKLSSLKSHIAAMKLNSLFDGTDFKLASCHQPSNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPLSKLPEI+EAGTHLSAAEFHSVLE+AGLLSDEET E
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEISEAGTHLSAAEFHSVLETAGLLSDEETPE 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNV TLDPEIRQYSDLPSWIDDNSEKL+GK ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVVTLDPEIRQYSDLPSWIDDNSEKLQGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRI+VGSSTQ
Sbjct: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTQ 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICGNP DDYSSRCRCKYCRMLVLVC NCQKKETLYVCELCRKHGKE EVISQF
Sbjct: 301 DIIGTCLICGNPFDDYSSRCRCKYCRMLVLVCDNCQKKETLYVCELCRKHGKEGEVISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QSS SE VLLQSELE GSQQLPWSNG R+PR+LRVLCLHGFRQNASSFKGRTASLAKKLK
Sbjct: 361 QSSKSEVVLLQSELENGSQQLPWSNGCRSPRKLRVLCLHGFRQNASSFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
           TMVEFVYVDAPHELSFIYHPRDSEP ETCVTS VQPN PPPLESCKKKFAWL+S+NAGER
Sbjct: 421 TMVEFVYVDAPHELSFIYHPRDSEPWETCVTSLVQPNHPPPLESCKKKFAWLVSNNAGER 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
           TET WEVADAPFDPLQYQKQTDGFE+SL YLKTVFSEKGPFDGILGFSQGAAMAAAVCSR
Sbjct: 481 TETNWEVADAPFDPLQYQKQTDGFEKSLAYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 532

Query: 541 KTSLKGAIDFRFAVLCSG 559
           K SLKGA++FRFAVLCSG
Sbjct: 541 KLSLKGAVEFRFAVLCSG 532

BLAST of HG10021654 vs. ExPASy TrEMBL
Match: A0A1S3CLM0 (rhodanese-like domain-containing protein 6 OS=Cucumis melo OX=3656 GN=LOC103502226 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.3e-277
Identity = 483/558 (86.56%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MANNE DDH  GVLLYYKYA+IPNLDDLVSFYQ NCTSLALVGRVRLASQGVNVT     
Sbjct: 1   MANNEDDDH--GVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKL SLKSHIAVMKLNSLFDGTDFKLA CHQP NDIV
Sbjct: 61  ---------------------VCGKLSSLKSHIAVMKLNSLFDGTDFKLASCHQPSNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPLSKLPEI+EAGTHLSAAEFHSVLESAGLLSDEET E
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEISEAGTHLSAAEFHSVLESAGLLSDEETPE 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNVETLDPEIRQYSDLPSWIDDNSEKLRGK ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRI+VGSST 
Sbjct: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTP 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICGNP DDYSSRCRCKYCRMLVLVC NCQ+KETLYVCELCRKHGKE EVISQF
Sbjct: 301 DIIGTCLICGNPFDDYSSRCRCKYCRMLVLVCDNCQEKETLYVCELCRKHGKEGEVISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QSS SE VLLQSEL      +PWSNG R+PR+LRVLCLHGFRQNAS FKGRTASLAKKLK
Sbjct: 361 QSSKSEVVLLQSEL------VPWSNGCRSPRKLRVLCLHGFRQNASGFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
           TMVEFVYVDAPHELSFIYHPRDS+P ETCVTSSVQP+ PPPLESCKKKFAWL+S NAG+R
Sbjct: 421 TMVEFVYVDAPHELSFIYHPRDSDPWETCVTSSVQPHRPPPLESCKKKFAWLVSDNAGKR 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
           TET WEVADAPFDPLQYQKQT GFE+SL YLKTVFSEKGPFDGILGFSQGAAM AAVCS+
Sbjct: 481 TETNWEVADAPFDPLQYQKQTTGFEKSLAYLKTVFSEKGPFDGILGFSQGAAMVAAVCSK 524

Query: 541 KTSLKGAIDFRFAVLCSG 559
           K SLKGA++FRFAVLCSG
Sbjct: 541 KPSLKGAVEFRFAVLCSG 524

BLAST of HG10021654 vs. ExPASy TrEMBL
Match: A0A6J1E0L1 (rhodanese-like domain-containing protein 6 OS=Momordica charantia OX=3673 GN=LOC111026061 PE=4 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 4.5e-274
Identity = 471/558 (84.41%), Postives = 498/558 (89.25%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           M NNE DD QYGVLLYYKYA IP+LDDLVSFY+ NC+SL LVGRVRLASQGVNVT     
Sbjct: 1   MVNNE-DDDQYGVLLYYKYAQIPDLDDLVSFYESNCSSLGLVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSL+SHI  MKLNSLFDGTDFKLA CHQPLNDIV
Sbjct: 61  ---------------------VCGKLCSLRSHITAMKLNSLFDGTDFKLASCHQPLNDIV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTLCSNPL KLPEITEAGTHLSAAEFHSVLESAGLLSDEET  
Sbjct: 121 ARECGFTSLSVRVVQELVTLCSNPLLKLPEITEAGTHLSAAEFHSVLESAGLLSDEETPR 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           H++DVVLLDARNLYETRVGKFQ+PNVETLDPEIRQYSDLPSWIDDNSEKLR K ILMYCT
Sbjct: 181 HNKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRAKKILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYI+SKG  FQNVFQLYGGIQRYLEQFPDGG FAGKNFVFDHR+SVGSST+
Sbjct: 241 GGIRCEMASAYIRSKGPDFQNVFQLYGGIQRYLEQFPDGGLFAGKNFVFDHRMSVGSSTE 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCLICG+P DDYSSRCRCK CRMLVLVC NCQKKETLYVCELC+KHGK+  +ISQF
Sbjct: 301 DIIGTCLICGDPFDDYSSRCRCKNCRMLVLVCDNCQKKETLYVCELCQKHGKDGGLISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLK 420
           QS+ SE V LQ+EL+IGSQQLPWSNG R+PR+LR+LCLHGFRQNASSFKGRTASLAKKLK
Sbjct: 361 QSTKSEVVSLQNELQIGSQQLPWSNGCRSPRKLRILCLHGFRQNASSFKGRTASLAKKLK 420

Query: 421 TMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGER 480
            MVEFVYVDAPHELSFIYHPRDSEP+E C+TSSVQPN PPPL  C+KKFAWLI+HNAGER
Sbjct: 421 IMVEFVYVDAPHELSFIYHPRDSEPQEACMTSSVQPNRPPPLGCCRKKFAWLIAHNAGER 480

Query: 481 TETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSR 540
            ET+WEVADAPFDPLQYQKQTDGFEESL YLKT+FSE+GPFDGILGFSQGAAM AAVCSR
Sbjct: 481 NETQWEVADAPFDPLQYQKQTDGFEESLAYLKTLFSEQGPFDGILGFSQGAAMVAAVCSR 531

Query: 541 KTSLKGAIDFRFAVLCSG 559
           KTSLKGAIDFRFAVLCSG
Sbjct: 541 KTSLKGAIDFRFAVLCSG 531

BLAST of HG10021654 vs. ExPASy TrEMBL
Match: A0A6J1EUT2 (rhodanese-like domain-containing protein 6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437801 PE=4 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 4.2e-264
Identity = 464/561 (82.71%), Postives = 488/561 (86.99%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MAN E DDHQ+GVLLYYKYA IP+LDDLVSFY+ NC SL LVGRVRLASQGVNVT     
Sbjct: 1   MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSLKSHIAVM LNSLFDGTDFKLA CHQP+ND+V
Sbjct: 61  ---------------------VCGKLCSLKSHIAVMNLNSLFDGTDFKLASCHQPMNDVV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTL SNPL KLPEITEAGTHLSAAEFHSVLE AGLLSDE+   
Sbjct: 121 ARECGFTSLSVRVVQELVTLRSNPLLKLPEITEAGTHLSAAEFHSVLERAGLLSDEKKRI 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           HS+DVVLLDARNLYETRVGKF++PN+ETLDPEIRQYSDL  WIDDNSEKLRGK ILMYCT
Sbjct: 181 HSKDVVLLDARNLYETRVGKFKTPNIETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGS FQNVFQLYGGIQRYLEQFP GGFF+GKNFVFDHRISVGSST+
Sbjct: 241 GGIRCEMASAYIKSKGSEFQNVFQLYGGIQRYLEQFPYGGFFSGKNFVFDHRISVGSSTE 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCL CG+P DDYSSRCRCKYCRMLVLVC NCQKK TLYVCELCRK+GK+   ISQF
Sbjct: 301 DIIGTCLSCGDPFDDYSSRCRCKYCRMLVLVCDNCQKKNTLYVCELCRKNGKDGGPISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGS--RTPRRLRVLCLHGFRQNASSFKGRTASLAKK 420
           QS+  E V  QS+ EIGSQQLPWSNG   R+PR+LRVLCLHGFRQNASSFKGRTASLAKK
Sbjct: 361 QSTKLEIVSQQSDHEIGSQQLPWSNGKVCRSPRKLRVLCLHGFRQNASSFKGRTASLAKK 420

Query: 421 LKTMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISH-NA 480
           LKTMVEFVYVDAPHELSFIYHPRD   RETCVTSSVQPNCPPP+E CKKKFAWLI+  N 
Sbjct: 421 LKTMVEFVYVDAPHELSFIYHPRD---RETCVTSSVQPNCPPPVEHCKKKFAWLIAEDNT 480

Query: 481 GERTETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAV 540
           GER+ETKWEVADA FDPLQYQKQTDGFEESL YLKTVFSEKGPFDGILGFSQGAAM AAV
Sbjct: 481 GERSETKWEVADALFDPLQYQKQTDGFEESLAYLKTVFSEKGPFDGILGFSQGAAMTAAV 532

Query: 541 CSRKTSLKGAIDFRFAVLCSG 559
           CSRK S+KGA+D RFAVLCSG
Sbjct: 541 CSRKMSVKGAVDVRFAVLCSG 532

BLAST of HG10021654 vs. ExPASy TrEMBL
Match: A0A6J1F017 (rhodanese-like domain-containing protein 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437801 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.9e-262
Identity = 463/561 (82.53%), Postives = 487/561 (86.81%), Query Frame = 0

Query: 1   MANNEGDDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLN 60
           MAN E DDHQ+GVLLYYKYA IP+LDDLVSFY+ NC SL LVGRVRLASQGVNVT     
Sbjct: 1   MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVT----- 60

Query: 61  YLFRSPFSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIV 120
                                VCGKLCSLKSHIAVM LNSLFDGTDFKLA CHQP+ND+V
Sbjct: 61  ---------------------VCGKLCSLKSHIAVMNLNSLFDGTDFKLASCHQPMNDVV 120

Query: 121 ARECGFTSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLE 180
           ARECGFTSLSVRVVQELVTL SNPL KLPEITEAGTHLSAAEFHSVLE AGLLSDE+   
Sbjct: 121 ARECGFTSLSVRVVQELVTLRSNPLLKLPEITEAGTHLSAAEFHSVLERAGLLSDEKKRI 180

Query: 181 HSRDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCT 240
           HS+DVVLLDARNLYETRVGKF++PN+ETLDPEIRQYSDL  WIDDNSEKLRGK ILMYCT
Sbjct: 181 HSKDVVLLDARNLYETRVGKFKTPNIETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCT 240

Query: 241 GGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQ 300
           GGIRCEMASAYIKSKGS FQNVFQLYGGIQRYLEQFP GGFF+GKNFVFDHRISVGSST+
Sbjct: 241 GGIRCEMASAYIKSKGSEFQNVFQLYGGIQRYLEQFPYGGFFSGKNFVFDHRISVGSSTE 300

Query: 301 DIIGTCLICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQF 360
           DIIGTCL CG+P DDYSSRCRCKYCRMLVLVC NCQ K TLYVCELCRK+GK+   ISQF
Sbjct: 301 DIIGTCLSCGDPFDDYSSRCRCKYCRMLVLVCDNCQ-KNTLYVCELCRKNGKDGGPISQF 360

Query: 361 QSSNSEAVLLQSELEIGSQQLPWSNGS--RTPRRLRVLCLHGFRQNASSFKGRTASLAKK 420
           QS+  E V  QS+ EIGSQQLPWSNG   R+PR+LRVLCLHGFRQNASSFKGRTASLAKK
Sbjct: 361 QSTKLEIVSQQSDHEIGSQQLPWSNGKVCRSPRKLRVLCLHGFRQNASSFKGRTASLAKK 420

Query: 421 LKTMVEFVYVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISH-NA 480
           LKTMVEFVYVDAPHELSFIYHPRD   RETCVTSSVQPNCPPP+E CKKKFAWLI+  N 
Sbjct: 421 LKTMVEFVYVDAPHELSFIYHPRD---RETCVTSSVQPNCPPPVEHCKKKFAWLIAEDNT 480

Query: 481 GERTETKWEVADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAV 540
           GER+ETKWEVADA FDPLQYQKQTDGFEESL YLKTVFSEKGPFDGILGFSQGAAM AAV
Sbjct: 481 GERSETKWEVADALFDPLQYQKQTDGFEESLAYLKTVFSEKGPFDGILGFSQGAAMTAAV 531

Query: 541 CSRKTSLKGAIDFRFAVLCSG 559
           CSRK S+KGA+D RFAVLCSG
Sbjct: 541 CSRKMSVKGAVDVRFAVLCSG 531

BLAST of HG10021654 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 671.8 bits (1732), Expect = 5.0e-193
Identity = 342/552 (61.96%), Postives = 397/552 (71.92%), Query Frame = 0

Query: 7   DDHQYGVLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSP 66
           D+ QYGVLLYYKY  +P+LD+LVSFY+ +C SL L+GRVRL+ +GVNVTV          
Sbjct: 16  DEEQYGVLLYYKYTSVPDLDELVSFYESSCNSLGLLGRVRLSPKGVNVTVG--------- 75

Query: 67  FSIWFLRKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECGF 126
                            GKL +L+ HIA  K N LF+GTDFKLA CH PLND VA ECGF
Sbjct: 76  -----------------GKLTALEEHIAAAKSNCLFEGTDFKLASCHHPLNDKVAEECGF 135

Query: 127 TSLSVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVV 186
           TSLS+RVV+ELVT    P  K PEI+ AG HLSAAEFHSVL+SA   S+      ++++V
Sbjct: 136 TSLSIRVVEELVTFSPCPPLKPPEISNAGKHLSAAEFHSVLQSANGKSE------NKELV 195

Query: 187 LLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCE 246
           LLDARNLYETR+GKF+S NVETLDPEIRQYSDLP+WID N+EK++GK++LMYCTGGIRCE
Sbjct: 196 LLDARNLYETRIGKFESENVETLDPEIRQYSDLPTWIDQNAEKMKGKNVLMYCTGGIRCE 255

Query: 247 MASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTC 306
           MASAYI+SKG+GF+N FQLYGGIQRYLEQFP GGFF GKNFVFDHRISVGSS +DIIG+C
Sbjct: 256 MASAYIRSKGAGFENTFQLYGGIQRYLEQFPSGGFFKGKNFVFDHRISVGSSKEDIIGSC 315

Query: 307 LICGNPSDDYSSRCRCKYCRMLVLVCANCQKKETLYVCELCRKHGKEDEVISQFQSSNSE 366
           L+C N  DDYS RCRC+ CRMLVLVC +C+ K  +Y+CELCRKHGK             E
Sbjct: 316 LLCNNTFDDYSPRCRCRLCRMLVLVCNHCRVKGDIYICELCRKHGK------------GE 375

Query: 367 AVLLQSELEIGSQQLPWSNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLKTMVEFV 426
             L    L   S+    SNG  T R+LR+LCLHGFRQNASSFKGRT SLAKKLK + E V
Sbjct: 376 VPLSLDPLNQPSE----SNGDNTRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELV 435

Query: 427 YVDAPHELSFIYHPRDSEPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGERTETKWE 486
           ++DAPHEL FIY                     PP   C KKFAWL+S +  + +ET W 
Sbjct: 436 FIDAPHELQFIYQ-----------------TATPPSGVCNKKFAWLVSSDFDKPSETGWT 495

Query: 487 VADAPFDPLQYQKQTDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAVCSRKTSLKG 546
           VA   FDPLQYQ QT+GF++SL YLKT F EKGPFDGILGFSQGAAMAAAVC ++  L G
Sbjct: 496 VAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFSQGAAMAAAVCGKQEQLVG 502

Query: 547 AIDFRFAVLCSG 559
            IDFRF VLCSG
Sbjct: 556 EIDFRFCVLCSG 502

BLAST of HG10021654 vs. TAIR 10
Match: AT2G40760.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.6e-21
Identity = 95/354 (26.84%), Postives = 156/354 (44.07%), Query Frame = 0

Query: 13  VLLYYKYAHIPNLDDLVSFYQYNCTSLALVGRVRLASQGVNVTVASLNYLFRSPFSIWFL 72
           V+ +YK+A  P   D     +  C  L + G + LA +G+N ++  +        +  F+
Sbjct: 106 VVSFYKFADFPEHADFRKPLKDLCEKLRVSGGIILAPEGINGSICGIRESVEEVLA--FI 165

Query: 73  RKTFKMIFLVCGKLCSLKSHIAVMKLNSLFDGTDFKLALCHQPLNDIVARECG---FTSL 132
           ++  ++       L  +++ ++  +  ++  G        H   + + A E     +  +
Sbjct: 166 QRDVRL-----NGLRQVETPVS-PEQEAIHHG--------HSSSSPLAAGEDAPFRWDHV 225

Query: 133 SVRVVQELVTLCSNPLSKLPEITEAGTHLSAAEFHSVLESAGLLSDEETLEHSRDVVLLD 192
            V++ +E+VTL    +  +  I   GT++S  E++       L+SD ET       V++D
Sbjct: 226 RVKLKKEIVTL---GIPSVSPIERVGTYVSPEEWNE------LISDPET-------VVID 285

Query: 193 ARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWI-------------------------- 252
            RN YETR+GKF+      +DP    + + PSW+                          
Sbjct: 286 VRNTYETRIGKFKG----AVDPCTTAFRNFPSWVENQFALKQEGNETQAKVEKEDFSEIT 345

Query: 253 --DDNSEKLRG-KHILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFP-DG 312
             +D +EK +    I MYCTGGIRCE AS+ + S+  GF+ V+ L GGI +YLE+ P   
Sbjct: 346 HKEDKAEKPKTLPRIAMYCTGGIRCEKASSLLLSQ--GFEEVYHLKGGILKYLEEVPKTE 405

Query: 313 GFFAGKNFVFDHRISVGSS-TQDIIGTCLICGNPSDD-------YSSRCRCKYC 326
             + G+ FVFD R+SV     Q     C  C  P  D       Y     C YC
Sbjct: 406 SLWEGECFVFDKRVSVEHGLAQGTHKLCYGCKQPISDEDMEAPEYEYGVSCPYC 421

BLAST of HG10021654 vs. TAIR 10
Match: AT1G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-12
Identity = 58/198 (29.29%), Postives = 85/198 (42.93%), Query Frame = 0

Query: 175 DEETLEHS-RDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSE----- 234
           DE +  +S +  +LLD RN YE  VG F+  +     PE+  + +    + D  E     
Sbjct: 211 DEASPSNSGKSYILLDVRNGYEWDVGHFRGAH----RPEVDCFRNTSFGLSDEKEAPSDP 270

Query: 235 ----KLRGKHILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAG 294
                     ILMYCTGGIRC++ S  ++ +  GF+N++ L GG+  YL++      + G
Sbjct: 271 LINVDKEKTDILMYCTGGIRCDVYSTVLRQR--GFKNLYTLKGGVSHYLKE-EGTAEWVG 330

Query: 295 KNFVFDHRIS--------------VGSSTQDIIG------------TCLICGNPSDDYSS 336
             FVFD R+S              VG +  D  G             C +C +   +   
Sbjct: 331 NLFVFDSRLSLPPAAYNDNVVDKAVGDNVVDEAGRTPQTPVDTSFARCYLCNSQVQELRH 390

BLAST of HG10021654 vs. TAIR 10
Match: AT1G17850.2 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-12
Identity = 58/198 (29.29%), Postives = 85/198 (42.93%), Query Frame = 0

Query: 175 DEETLEHS-RDVVLLDARNLYETRVGKFQSPNVETLDPEIRQYSDLPSWIDDNSE----- 234
           DE +  +S +  +LLD RN YE  VG F+  +     PE+  + +    + D  E     
Sbjct: 213 DEASPSNSGKSYILLDVRNGYEWDVGHFRGAH----RPEVDCFRNTSFGLSDEKEAPSDP 272

Query: 235 ----KLRGKHILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFPDGGFFAG 294
                     ILMYCTGGIRC++ S  ++ +  GF+N++ L GG+  YL++      + G
Sbjct: 273 LINVDKEKTDILMYCTGGIRCDVYSTVLRQR--GFKNLYTLKGGVSHYLKE-EGTAEWVG 332

Query: 295 KNFVFDHRIS--------------VGSSTQDIIG------------TCLICGNPSDDYSS 336
             FVFD R+S              VG +  D  G             C +C +   +   
Sbjct: 333 NLFVFDSRLSLPPAAYNDNVVDKAVGDNVVDEAGRTPQTPVDTSFARCYLCNSQVQELRH 392

BLAST of HG10021654 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 1.2e-05
Identity = 41/157 (26.11%), Postives = 61/157 (38.85%), Query Frame = 0

Query: 384 SNGSRTPRRLRVLCLHGFRQNASSFKGRTASLAKKLKTMVEFVYVDAPHELSFIYHPRDS 443
           S G    R+ R LCLHGFR +    K +     K +   ++ V++DAP            
Sbjct: 3   SEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQ-------- 62

Query: 444 EPRETCVTSSVQPNCPPPLESCKKKFAWLISHNAGERTETKWEVADAPFDPLQYQK---Q 503
                   S V+    PP       + W                        Q+ K   +
Sbjct: 63  ------GKSDVEGIFDPPY------YEW-----------------------FQFNKEFTE 116

Query: 504 TDGFEESLEYLKTVFSEKGPFDGILGFSQGAAMAAAV 538
              FE+ LEYL+    + GPFDG++GFSQGA ++  +
Sbjct: 123 YTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGL 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895686.11.2e-29490.88rhodanese-like domain-containing protein 6 [Benincasa hispida][more]
XP_011654855.18.1e-28688.53rhodanese-like domain-containing protein 6 [Cucumis sativus] >KGN65012.1 hypothe... [more]
XP_008464312.16.8e-27786.56PREDICTED: rhodanese-like domain-containing protein 6 [Cucumis melo][more]
XP_022159722.19.2e-27484.41rhodanese-like domain-containing protein 6 [Momordica charantia][more]
XP_022931639.18.7e-26482.71rhodanese-like domain-containing protein 6 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94AC17.0e-19261.96Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=ST... [more]
Q5T7W72.0e-4535.59Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Homo... [more]
Q5RCP14.5e-4535.31Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Pong... [more]
Q3U2694.9e-4434.84Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Mus ... [more]
Q65EW54.3e-3230.42tRNA uridine(34) hydroxylase OS=Bacillus licheniformis (strain ATCC 14580 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A0A0LW433.9e-28688.53Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G180760 P... [more]
A0A1S3CLM03.3e-27786.56rhodanese-like domain-containing protein 6 OS=Cucumis melo OX=3656 GN=LOC1035022... [more]
A0A6J1E0L14.5e-27484.41rhodanese-like domain-containing protein 6 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1EUT24.2e-26482.71rhodanese-like domain-containing protein 6 isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1F0173.9e-26282.53rhodanese-like domain-containing protein 6 isoform X2 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
AT1G09280.15.0e-19361.96CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas... [more]
AT2G40760.11.6e-2126.84Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G17850.12.3e-1229.29Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G17850.22.3e-1229.29Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT4G24380.11.2e-0526.11INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainSMARTSM00450rhod_4coord: 169..278
e-value: 1.2E-7
score: 41.4
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 181..281
score: 12.868606
NoneNo IPR availableGENE3D3.30.70.100coord: 1..61
e-value: 7.8E-12
score: 47.2
NoneNo IPR availableCDDcd01518RHOD_YceAcoord: 155..274
e-value: 2.86074E-40
score: 139.637
IPR022111Rhodanase, C-terminalPFAMPF12368Rhodanese_Ccoord: 284..348
e-value: 1.3E-15
score: 57.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 389..566
e-value: 9.0E-40
score: 138.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 388..560
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 145..306
e-value: 3.3E-40
score: 139.1
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 149..295
IPR005645Serine hydrolase FSHPFAMPF03959FSH1coord: 392..562
e-value: 1.7E-33
score: 116.1
IPR040503tRNA uridine(34) hydroxylase, N-terminalPFAMPF17773UPF0176_Ncoord: 12..58
e-value: 1.5E-8
score: 34.8
IPR020936tRNA uridine(34) hydroxylasePANTHERPTHR43268THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 1..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10021654.1HG10021654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding