HG10021454 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021454 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 586.6 bits (1511), Expect = 1.4e-163
Identity = 289/346 (83.53%), Postives = 311/346 (89.88%), Query Frame = 0

Query: 1   MLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPV 60
           +LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+FGVVVAWKVKLVPV
Sbjct: 194 ILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNFGVVVAWKVKLVPV 253

Query: 61  PATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 120
           P TVTICA KRTLE+G I+LI+QWQYV NKL+E+LFL IFL GGK SAQGGGK+NP ALF
Sbjct: 254 PETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNSAQGGGKVNPTALF 313

Query: 121 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 180
           FSLFLGKADEL+ ILK+ FPELGLTKEDCKE SWI S++ AANRFQ  DQ LE LLNRT 
Sbjct: 314 FSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQIEDQPLEALLNRTP 373

Query: 181 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 240
            ANGS KIKSDYVKE ITKV IEGIWERLKSQDI GVTLALIP+GGRM QIS+SEIPFSH
Sbjct: 374 LANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGRMGQISDSEIPFSH 433

Query: 241 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 300
           RAG  YQIGYIVGW+EQG+ AEKR++NWIREIY+YMTPFVSKSPR+AYVNYRD DIGSNN
Sbjct: 434 RAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAAYVNYRDHDIGSNN 493

Query: 301 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           K+GK SYKRAR WG KYFG NNFNRLV +KTKVDP NFFRHE SIP
Sbjct: 494 KYGKVSYKRARVWGLKYFG-NNFNRLVRLKTKVDPSNFFRHEQSIP 538

BLAST of HG10021454 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 583.9 bits (1504), Expect = 8.9e-163
Identity = 285/346 (82.37%), Postives = 308/346 (89.02%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVP
Sbjct: 195 LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 254

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVTIC   RT ++G IKLI+QWQYVANKLDE+LFL I L GGK+SAQ GGKINP ALFF
Sbjct: 255 ATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKISAQ-GGKINPTALFF 314

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           +LFLGKADEL+TILK  FPEL L KEDCKETSWIES++Y AN  Q  DQ LE  LNRT  
Sbjct: 315 TLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQIDDQPLEAFLNRTPL 374

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
           ANGSFKIKSDYVKEPITK AIEGIW+RLKSQDI GV L LIP+GGRMSQISESEIPF HR
Sbjct: 375 ANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGRMSQISESEIPFPHR 434

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
            GNLY+IGYI+GWEEQG+ AEKR+LNWIREIY+YMTPFVSKSPR+AYVNYRD DIGSN+K
Sbjct: 435 IGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNDK 494

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 348
           +GKTSYKRA  WG KYFG NNFNRLVHVK +VDPY+FFRHE SIP+
Sbjct: 495 YGKTSYKRACIWGKKYFG-NNFNRLVHVKNEVDPYDFFRHEQSIPS 538

BLAST of HG10021454 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 567.8 bits (1462), Expect = 6.6e-158
Identity = 278/348 (79.89%), Postives = 311/348 (89.37%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVP
Sbjct: 194 LLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 253

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVTIC+ KR+LE+  IKLI+QWQYVANKL+EELFL IF  GGKVSAQ GGKINPIALFF
Sbjct: 254 ATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFF 313

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           +LFLG  +ELM IL +TFPELGLTKE+CKE SWIES+  AANRFQ  DQ LE LLNRT  
Sbjct: 314 TLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPI 373

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
            +GSFK+KSDYVKEP+TK AI+GIW+RL+SQDI GVTLA+IP+GGRM+QISES+IPF HR
Sbjct: 374 THGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHR 433

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
           AG LYQIGYI+GWEE+G+ AEKR+LNWIREIY+YMTPFVSKSPR+AYVNYRD DIGSNNK
Sbjct: 434 AGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNK 493

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPTPS 350
           +GK +YK+A  +GSKYFG NNFNRLV VK+ VDPYNFF HE SIP  S
Sbjct: 494 YGKINYKQACVFGSKYFG-NNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

BLAST of HG10021454 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-155
Identity = 276/349 (79.08%), Postives = 310/349 (88.83%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP
Sbjct: 207 LLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 266

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           A+VTIC+ KR++E+  IKLI++WQYVANKL+E+LFL I   GGKVSAQGGGKINPIALFF
Sbjct: 267 ASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFF 326

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRT-L 181
            LFLG  +ELM IL +TFPELG TKE+CKE SWIES+  AANRFQ  DQ LE LLNRT +
Sbjct: 327 PLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPI 386

Query: 182 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 241
           +   SFK+KSDYVKEP+TK AIEGIW+RL+SQDI GVTLALIP+GGRMS+ISES IPFSH
Sbjct: 387 NHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSH 446

Query: 242 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 301
           RAG LYQIGYI+GW+E+G+ AE+R+LNWIREIY+YMTPFVSKSPR+AYVNYRD DIGSNN
Sbjct: 447 RAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNN 506

Query: 302 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPTPS 350
           K+GK +YK+A  +GSKYFG NNFNRLV VKTKVDPYNFF HE SIP  S
Sbjct: 507 KYGKINYKQACVFGSKYFG-NNFNRLVKVKTKVDPYNFFWHEQSIPLSS 554

BLAST of HG10021454 vs. NCBI nr
Match: XP_011659204.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical protein Csa_023357 [Cucumis sativus])

HSP 1 Score: 557.0 bits (1434), Expect = 1.2e-154
Identity = 271/345 (78.55%), Postives = 300/345 (86.96%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYGLAADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVP
Sbjct: 194 LLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 253

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVTIC   RTLE+  IKL++QWQYVANKLDE+LFLGI L+GGK+SAQ G KINPIALFF
Sbjct: 254 ATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQ-GDKINPIALFF 313

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           SLFLGKADELM IL +TFP+LGLTKE+CKETSWIES +Y  N  Q  DQ LE LLNRT  
Sbjct: 314 SLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRTPL 373

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
           A G+ K+KSDYVKEPI K  IE IW+RL+SQDI G  L  +P+GGRMSQIS+SEIPFSHR
Sbjct: 374 ATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHR 433

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
           AGNLY+IGY+ GW E G+ AEK +LNWIR+IY YMTPFVSKSPR+AYVNYRD DIGSN+K
Sbjct: 434 AGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSK 493

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           +GKTSYKRAR WG KYFG NNFNR+V+VK KVDPYNFFRHE SIP
Sbjct: 494 YGKTSYKRARVWGLKYFG-NNFNRMVYVKNKVDPYNFFRHEQSIP 536

BLAST of HG10021454 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 5.9e-93
Identity = 172/346 (49.71%), Postives = 243/346 (70.23%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           ++RK+GL ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFGV++AWK+KLVPVP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVT+    +TLE    K++ +W+ +A+KLD++LF+ + +     + + G +   ++ + 
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQ 315

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           + FLG ++ L+ +++++FPELGLTK+DC E SWI+S +Y A    S   A E LL     
Sbjct: 316 AQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSA--APEALLAGKSL 375

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
               FK KSD+VKEPI    +EG+WER   +D         P+GG MS+ISESEIPF HR
Sbjct: 376 FKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHR 435

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
            G L++I ++  W++ G V+E+R++ WIRE+Y+YM  +VSK+PR AYVNYRD D+G+N  
Sbjct: 436 NGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE- 495

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 348
            G+T    AR+WG+KY+   NF RLV +K + DP NFFRHE S+PT
Sbjct: 496 -GETD---AREWGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of HG10021454 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-92
Identity = 169/349 (48.42%), Postives = 236/349 (67.62%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK+KLV VP
Sbjct: 193 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVP 252

Query: 62  ATVTICATKRTLE-DGTIKLINQWQYVANKLDEELFLGIFLIGGKVS-AQGGGKINPIAL 121
           +  TI + K+ +E  G +KL N+WQ +A K D++L L    I   ++   G  K      
Sbjct: 253 SKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGY 312

Query: 122 FFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLLN 181
           F S+F G  D L+ ++ ++FPELG+ K DCKE SWI+++++ +    F + +   E LL+
Sbjct: 313 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLD 372

Query: 182 RTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIP 241
           R+     +F IK DYVK+PI + A+  I E+L  +D+G     L P+GG M +ISES IP
Sbjct: 373 RSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIP 432

Query: 242 FSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIG 301
           F HRAG +Y++ Y   WE+Q     ++++NW+R +Y + TP+VS++PR AY+NYRD D+G
Sbjct: 433 FPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 492

Query: 302 SNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
             N     +Y +AR WG KYFG  NFNRLV VKTKVDP NFFR+E SIP
Sbjct: 493 KTNHASPNNYTQARIWGEKYFG-KNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of HG10021454 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-92
Identity = 175/349 (50.14%), Postives = 241/349 (69.05%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFG++VAWK++LV VP
Sbjct: 193 LMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVP 252

Query: 62  ATVTICATKRTLE-DGTIKLINQWQYVANKLDEELFLGIFLIGGKVS-AQGGGKINPIAL 121
            + T+ + K+ +E    +KL+N+WQ +A K D++L L    I   ++  QG  K      
Sbjct: 253 KS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTY 312

Query: 122 FFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLLN 181
           F S+FLG  D L+ ++ ++FPELG+ K DC++ SWI++ ++ +    + + +   E LL+
Sbjct: 313 FSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLD 372

Query: 182 RTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIP 241
           R+   NG+FKIK DYVK+PI +     I E+L  +DIG    AL P+GG M +ISES IP
Sbjct: 373 RSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 432

Query: 242 FSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIG 301
           F HRAG LY++ YI  WE+Q     +++LNWIR IY +MTP+VSK+PR AY+NYRD DIG
Sbjct: 433 FPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 492

Query: 302 SNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
            N+     +Y +AR WG KYFG  NF+RLV VKT VDP NFFR+E SIP
Sbjct: 493 INDPKNPNNYTQARIWGEKYFG-KNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of HG10021454 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.2e-91
Identity = 173/346 (50.00%), Postives = 236/346 (68.21%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           ++RKYGL ADNV+DA +VDANG+  DR +MGED FWAIRGG GGSFG+++AWK+KLVPVP
Sbjct: 198 MMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVP 257

Query: 62  ATVTICATKRTL-EDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 121
            TVT+    +TL +D   K+I++WQ VA+KL EELF+ +      V+  GG K      +
Sbjct: 258 KTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNK-TVTTSY 317

Query: 122 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 181
            +LFLG    LM ++K++FPELGLT +DC E SW+ES  Y +  F +       L  ++ 
Sbjct: 318 NALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG-FPTHTPTNVLLQGKSP 377

Query: 182 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 241
               SFK KSD+VK PI +  ++GI+++L  +DI    +   P+GG M++I ES+IPF H
Sbjct: 378 FPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIPESQIPFPH 437

Query: 242 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 301
           R G L+++ Y+  W +       R++NWIR++Y+YMTP+VS +PR AYVNYRD D+G N 
Sbjct: 438 RKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNT 497

Query: 302 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           K  KT  K+A+ WG+ YF  NNFNRL+ +K KVDP NFFRHE SIP
Sbjct: 498 KDVKTCIKQAQVWGANYF-KNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of HG10021454 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.6e-90
Identity = 166/349 (47.56%), Postives = 233/349 (66.76%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK+KLV VP
Sbjct: 193 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVP 252

Query: 62  ATVTICATKRTLE-DGTIKLINQWQYVANKLDEELFLGI-FLIGGKVSAQGGGKINPIAL 121
           +  TI + K+ +E  G +KL N+WQ +A K D++L L   F         G  K      
Sbjct: 253 SKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGY 312

Query: 122 FFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLLN 181
           F S+FLG  D L+ ++ ++FPELG+ K DCKE SWI+++++ +    + + +   E LL+
Sbjct: 313 FSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLD 372

Query: 182 RTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIP 241
           R+     +F IK DYVK+ I + A+  I E+L  +++G     L P+GG M +ISES IP
Sbjct: 373 RSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIP 432

Query: 242 FSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIG 301
           F HRAG +Y++ Y   WE+Q     ++++NW+R +Y + TP+VS++PR AY+NYRD D+G
Sbjct: 433 FPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 492

Query: 302 SNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
             N     +Y +AR WG KYFG  NFNRLV VKTK DP NFFR+E SIP
Sbjct: 493 KTNPESPNNYTQARIWGEKYFG-KNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of HG10021454 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.1e-156
Identity = 276/349 (79.08%), Postives = 310/349 (88.83%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP
Sbjct: 207 LLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 266

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           A+VTIC+ KR++E+  IKLI++WQYVANKL+E+LFL I   GGKVSAQGGGKINPIALFF
Sbjct: 267 ASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFF 326

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRT-L 181
            LFLG  +ELM IL +TFPELG TKE+CKE SWIES+  AANRFQ  DQ LE LLNRT +
Sbjct: 327 PLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPI 386

Query: 182 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 241
           +   SFK+KSDYVKEP+TK AIEGIW+RL+SQDI GVTLALIP+GGRMS+ISES IPFSH
Sbjct: 387 NHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSH 446

Query: 242 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 301
           RAG LYQIGYI+GW+E+G+ AE+R+LNWIREIY+YMTPFVSKSPR+AYVNYRD DIGSNN
Sbjct: 447 RAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNN 506

Query: 302 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPTPS 350
           K+GK +YK+A  +GSKYFG NNFNRLV VKTKVDPYNFF HE SIP  S
Sbjct: 507 KYGKINYKQACVFGSKYFG-NNFNRLVKVKTKVDPYNFFWHEQSIPLSS 554

BLAST of HG10021454 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.4e-155
Identity = 270/345 (78.26%), Postives = 303/345 (87.83%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVP
Sbjct: 194 LLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 253

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVTIC   RTLE+  IKLI+QWQYVANKLDE+LFLGI L+GGK+ AQ G KINPIALFF
Sbjct: 254 ATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQ-GDKINPIALFF 313

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           SLFLGKADELM IL +TFP+LGLTKE+CKETSWIES +Y  N  Q  DQ LE LLNRT  
Sbjct: 314 SLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRTPL 373

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
           ANG+ K+KSDYVKEPI+K  I+ +W+RLKSQDI G  L  IP+GGRMSQISESEIPFSHR
Sbjct: 374 ANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMSQISESEIPFSHR 433

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
           AGNLY+IGY+ GW E G+ AEK +LNWIR+IY+YMTPFV+KSPR+AYVNYRD DIGSN+K
Sbjct: 434 AGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNSK 493

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           +GKTSYKRA  WGSKYFG +NF+RLV+VK +VDPYNFFRHE SIP
Sbjct: 494 YGKTSYKRAHVWGSKYFG-SNFDRLVYVKNEVDPYNFFRHEQSIP 536

BLAST of HG10021454 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.4e-155
Identity = 270/345 (78.26%), Postives = 303/345 (87.83%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVP
Sbjct: 194 LLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 253

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVTIC   RTLE+  IKLI+QWQYVANKLDE+LFLGI L+GGK+ AQ G KINPIALFF
Sbjct: 254 ATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQ-GDKINPIALFF 313

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           SLFLGKADELM IL +TFP+LGLTKE+CKETSWIES +Y  N  Q  DQ LE LLNRT  
Sbjct: 314 SLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRTPL 373

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
           ANG+ K+KSDYVKEPI+K  I+ +W+RLKSQDI G  L  IP+GGRMSQISESEIPFSHR
Sbjct: 374 ANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMSQISESEIPFSHR 433

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
           AGNLY+IGY+ GW E G+ AEK +LNWIR+IY+YMTPFV+KSPR+AYVNYRD DIGSN+K
Sbjct: 434 AGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNSK 493

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           +GKTSYKRA  WGSKYFG +NF+RLV+VK +VDPYNFFRHE SIP
Sbjct: 494 YGKTSYKRAHVWGSKYFG-SNFDRLVYVKNEVDPYNFFRHEQSIP 536

BLAST of HG10021454 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 266/349 (76.22%), Postives = 298/349 (85.39%), Query Frame = 0

Query: 1   MLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPV 60
           ++LRK+GLAADNVIDAYLVDANG+F DRE MGEDLFWAIRGGGGGSFG+VVAWKVKLVPV
Sbjct: 195 LMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 254

Query: 61  PATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 120
           PATVT C   RTLE+G IKLI++WQYVANKLDE LFLGI L+GG  +AQGG K NP A F
Sbjct: 255 PATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASF 314

Query: 121 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 180
           FSLFLGKADE   IL  TFPELGL KE+C E+SWIES+L +A  F+ GD +LE LLNRT 
Sbjct: 315 FSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAG-FKIGD-SLEALLNRTP 374

Query: 181 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 240
             NGS KIKSDYVKEPI++  IEGIWERLKS+D+G   L  +P+GGRMSQISESEIPFSH
Sbjct: 375 LTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEIPFSH 434

Query: 241 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 300
           RAGNLY+IGYIV W++Q +  EKR+L WIRE+Y+YMTPFVSKSPRSAYVNYRD DIGSNN
Sbjct: 435 RAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDIGSNN 494

Query: 301 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPTPS 350
           K+GKTS+KRA  WG KYFG NNFNRLVHVKTKVDPY+FFRHE SIPT S
Sbjct: 495 KYGKTSFKRASVWGWKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of HG10021454 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.1e-146
Identity = 254/346 (73.41%), Postives = 294/346 (84.97%), Query Frame = 0

Query: 1   MLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPV 60
           +LLRKYGLAADNVIDAYLVDA+G+FH+RESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPV
Sbjct: 194 LLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPV 253

Query: 61  PATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 120
           PATVT+C+  RTLE+G +K+ ++WQYVANKLDE LFLGI  +GGK+++Q GGK NP+A F
Sbjct: 254 PATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVATF 313

Query: 121 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 180
           FSLFLGKADEL++IL   FPEL L K+DC ETSWIES++     F + +  LE LL+RT 
Sbjct: 314 FSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT-ELTLEALLSRTP 373

Query: 181 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 240
             N S KIKSDYVKEP+++  ++GIW+RL SQDI G  +  +P+GGRMSQISESE PFSH
Sbjct: 374 LTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETPFSH 433

Query: 241 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 300
           RAG LY+IGYI GWEEQGI AEKR+L WIRE+Y YMTPFVSKSPR+AY NYRD DIGSNN
Sbjct: 434 RAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDLDIGSNN 493

Query: 301 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           KFGKTSYK+A  WG KYFG NNFNRLVHVKTKVDPY+FFRHE SIP
Sbjct: 494 KFGKTSYKQASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIP 537

BLAST of HG10021454 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 342.4 bits (877), Expect = 4.2e-94
Identity = 172/346 (49.71%), Postives = 243/346 (70.23%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           ++RK+GL ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFGV++AWK+KLVPVP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           ATVT+    +TLE    K++ +W+ +A+KLD++LF+ + +     + + G +   ++ + 
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQ 315

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
           + FLG ++ L+ +++++FPELGLTK+DC E SWI+S +Y A    S   A E LL     
Sbjct: 316 AQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSA--APEALLAGKSL 375

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
               FK KSD+VKEPI    +EG+WER   +D         P+GG MS+ISESEIPF HR
Sbjct: 376 FKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHR 435

Query: 242 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 301
            G L++I ++  W++ G V+E+R++ WIRE+Y+YM  +VSK+PR AYVNYRD D+G+N  
Sbjct: 436 NGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE- 495

Query: 302 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 348
            G+T    AR+WG+KY+   NF RLV +K + DP NFFRHE S+PT
Sbjct: 496 -GETD---AREWGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of HG10021454 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.1e-92
Identity = 173/346 (50.00%), Postives = 236/346 (68.21%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           ++RKYGL ADNV+DA +VDANG+  DR +MGED FWAIRGG GGSFG+++AWK+KLVPVP
Sbjct: 198 MMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVP 257

Query: 62  ATVTICATKRTL-EDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 121
            TVT+    +TL +D   K+I++WQ VA+KL EELF+ +      V+  GG K      +
Sbjct: 258 KTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNK-TVTTSY 317

Query: 122 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 181
            +LFLG    LM ++K++FPELGLT +DC E SW+ES  Y +  F +       L  ++ 
Sbjct: 318 NALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG-FPTHTPTNVLLQGKSP 377

Query: 182 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 241
               SFK KSD+VK PI +  ++GI+++L  +DI    +   P+GG M++I ES+IPF H
Sbjct: 378 FPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIPESQIPFPH 437

Query: 242 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 301
           R G L+++ Y+  W +       R++NWIR++Y+YMTP+VS +PR AYVNYRD D+G N 
Sbjct: 438 RKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNT 497

Query: 302 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
           K  KT  K+A+ WG+ YF  NNFNRL+ +K KVDP NFFRHE SIP
Sbjct: 498 KDVKTCIKQAQVWGANYF-KNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of HG10021454 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 331.3 bits (848), Expect = 9.7e-91
Identity = 172/347 (49.57%), Postives = 237/347 (68.30%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           ++RK+GL ADNV+DA +VDA+G+  +R +MGED+FWAIRGGGGGSFGV++AWK+KLVPVP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
             VT+    RTLE    KL+ +WQ VA+KLDE+LF+ + +     S     K   I+  +
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII--QPTSKTPKSKERTISTSY 317

Query: 122 -SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTL 181
              FLG A+ L+ +++++FP+LGLTK+DC ETSWI+S +Y A  F S   + E LL+   
Sbjct: 318 QGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG-FPSTAPS-EALLDGKS 377

Query: 182 SANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSH 241
                FK KSDYV+EPI    +EG+WE+L  +D         P+GG M++I E+E PF H
Sbjct: 378 LFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGMMAKIPETETPFPH 437

Query: 242 RAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNN 301
           R+G L++I ++  W++ G  +E +++ W+RE+Y+YM  +VSKSPRSAYVNYRD D+G N 
Sbjct: 438 RSGTLFKIQWLTLWQD-GKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG 497

Query: 302 KFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 348
           K        AR+WG++YF   NF RLV +K K DP NFFRHE SIPT
Sbjct: 498 KGSD-----AREWGNRYF-KGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of HG10021454 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 330.1 bits (845), Expect = 2.2e-90
Identity = 167/348 (47.99%), Postives = 236/348 (67.82%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           LLRKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFGV+++WKVKLV VP
Sbjct: 191 LLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVP 250

Query: 62  ATVTICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALFF 121
           +T+T+   ++T +   +++I +WQY A+K+ ++LF+       + + +   K    ALF 
Sbjct: 251 STITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHALFT 310

Query: 122 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 181
            L++G  + L+ ++++ FPELGL KE C+E SWIES L+ A+ F  G ++L  L NR  +
Sbjct: 311 GLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKG-ESLGVLTNRERT 370

Query: 182 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 241
           +  SFK K D+V+EPI + AI+ IW RL++ +     + L PFGG+MS+++E E PF HR
Sbjct: 371 SL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHR 430

Query: 242 AGNLYQIGYIVGW---EEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGS 301
            GNLY+I Y+  W   E++      + L W+  +Y +MTP+VSKSPR AYVN++D D+G 
Sbjct: 431 GGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGM 490

Query: 302 NNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 347
                KT Y+  + WG KYF  NNF RLV VKT+VDP +FF  E SIP
Sbjct: 491 YLGKKKTKYEEGKSWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of HG10021454 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-89
Identity = 169/348 (48.56%), Postives = 232/348 (66.67%), Query Frame = 0

Query: 2   LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVP 61
           L+RKYGLAADNV+DA +VDANG+  DR SMGEDLFWAIRGG GGSFG++++WK+KLVPVP
Sbjct: 200 LMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVP 259

Query: 62  ATVTICATKRTLE-DGTIKLINQWQYVANKLDEELFLGIFLIGGKVSAQGGGKINPIALF 121
            T+T+    +T E D + K++++WQ +A+ L +ELFL +F     VS     K   +A +
Sbjct: 260 ETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFF---TVSGNKANKTVTMA-Y 319

Query: 122 FSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLN-RT 181
              FLG+   LM ++K+ FPELGLT++DC E SWI+S +Y +    +    +E LL  ++
Sbjct: 320 IGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKS 379

Query: 182 LSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFS 241
                 FK KSD+ K+PI  + +EG++++L  +D     +   P+GG+M +I ESEIPF 
Sbjct: 380 PIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED--AALVIWTPYGGKMDKIPESEIPFP 439

Query: 242 HRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSN 301
           HR G  + I Y   W +       R   WIRE+Y YMTP+VS +PR AYVNYRD D+G N
Sbjct: 440 HRNGTNFMIQYYRSWSDSE-KRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQN 499

Query: 302 NKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 348
               K+++  A+ WG+ YF  +NFNRLV +K+KVDP NFFRHE SIPT
Sbjct: 500 KDNSKSNFIEAKIWGANYF-KDNFNRLVRIKSKVDPDNFFRHEQSIPT 539