HG10021005 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10021005
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGDSL esterase/lipase
LocationChr05: 4426264 .. 4429916 (-)
RNA-Seq ExpressionHG10021005
SyntenyHG10021005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGACCCAGCGACGTCGTTTCGCCGTGGCCGCCACTTTCGTCGTCGTACTCGTATTTCTGAAATGTTCCAATGAGATGGTTGTAGCGGCAAGTAATGGGGTTTCGGCTATTTTTGTGTTTGGGGACTCTCTTGTGGACGTTGGGAACAATAATTTTCTTCACTCAGCTGCAAGAGCCAATTATTACCCTTATGGGATTGATTTTCATGCTGGTCCTACTGGGAGATTCTCTAATGGACGAACAGTTATAGATATGTTTGGTACATTTAATATTAACTTAAAACACCCCTTTTTTCTTTTAGTCATTCAAAATTTCTGATATTAATTTTAAGAGTTTGTGTGATTAACATGCAGTGGACATGCTTGGAATTCCCAACGCTCCAGAATTTGCCAATCCGGACACTTCCGGAGACAGAATATATAACGGTGTCAACTATGCTTCGGCAGCTGCCGGCATACTCGACGAGACCGGCAGACACTATGTATGTAATATTAGTCGGATGTTTTTCTTTTCCATGTTTGGGAGTAATTTTAGAATGACTAAATAATTTTTAAGATTTTTAAATTCTTTTTGAAACATGCATTTTTTTTAAATAAATAGGAGCATAAGAATTTTAACTTATGACCTTAAGAAAAAGTTAGTACGTGTTAATTACTGTTAAACTATGCTCAACTTTACCTCTTGAAATTATTCGTCCAAATCTATTTAATGTTTGATTTTATACTTCTAAATATGAATTTTATTTTTAAGACTTGAATTGATTGGGAGTAATTTTGAATATAAATAGGGTCGTTTAATTTTACTGTTTGAAGGTATGTTTTTGAGTAATCTTGAATGTAATAAAAATGATTTTAACCCTTTCAAAATCACTCTCAAACATTCATATATCAATTGAATATTTTTTTTGAATTTATAGTAAAACTAATTTTAGTTAAATTTAATTTGTTTTTTTGGTCATTGATTTTCACCATCTATTTACCATGATTTTTTTTTTTTGTTCAAAACCAAAAACAAGTTTTTAAAGCTATGTTTGATAACTTATTTCGTTTTTTTGTTTTTTTAGTTTTGAAAATTGGTTTGGATTTTGTAAACACTCTTATAATATAGACAACAAAACAAAGTAATCAAATAATGCATATAAGCTTAATTTTAAAAAATAACTAAATAATTATCAAACTGGCTTAAAAGCATAATTTCTTTTTAAATTTTTAAAAAACTTGATAACATAACAAAGACATTCACAAGTAAAGTAGGTAGTATTTATAAGCTTGAGCATAAATTTATACCAATTATTAGGAGGAGAATGATTAAGTGCAGTCTAGGATGCACCTATTCTCGTACTCCTCTCTTTTTTACTCAATAATAAATTTTAAAAATTATCTTTTTTTAAAAAAATCTGTGTAATGTGACAAAATTTTATTGGGTGTTGGCCGAGCTGCACCTAAAAAAACGTAGAATGTATTGATGGTATTGGTCTATTGCGTGAGTTACAGGGAGATAGGTACAGTCTAAGCCAGCAAGTGGTGAATTTTGAAAGCACTTTGGATGAGTTAAGGAGATCAATGGGGAGTTGGAATTTGACTTGTTACTTATCAAAATCCATAGCCTTTTTAGCGTTTGGAAGCAACGATTACATAAACAACTACTTGTTACCGAATCTGTACACTTCCAGATTCAGATACAATTCTAACCAATTCGCCAACTTTCTCCTTAACCGCTATTCTCGACAGCTTCTCGTAAGTTTCTCTTTCATATATCCAACCGTCTTACCGTAAAAAAAACTCTTAGTTACAAATTACTAAGAAATAATAATACCATCCATGCATATTGTGTGAATGCATGCACAGGCACTGCAAAGCGTAGGGTTGAGAAAGTTCGTGGTGGCCGGAGTGGGGCCGTTGGGTTGTATCCCGAACCAGAGGGCGACCGGCTTTACCATGCCAGGGCGGTGCGTCGATAATGTGAATGAGATGCTGGGAGCTTTTAATGAAGGGCTGAAATCGTTAGTAACTCAGTTGAATAGCCAGCATCCTGATTCCACCTTCGTGTATACAAATATCTATGGCATTTTTGGCGACATCTTGAACAACCCTGAAACCTACGGTGAGACATCCTCATATATATGTTGAAAACAAATAAAATGGACTAGGCTCAGACGGTTTTAGTTCAAGTTTTTCACAAAAAAATTTGTTTGGTAATTCGTTTTGAATCTAATTTTTTTTTTTTTAATTCGTTTTGAATCTAATTTTTATATGATTCTTAAGTTTCACAGTGTTGCACTTTTAACTTTGAATTTTGAGTTCAATTTTCATTTGATTTTTAAGTTATAAAATGTTACATTATTTGATCTTGTTCAAATTTATTCCTATATTTTGATCCTCTCGATGTTAATGTTTTAATTAATTTTAAATTTCATCACTAACTAAAATCAAACAATAAAATTTAACGTCATAATTATTTTAAATTAGTTACCTTAGTTAACATTAAAAATAAAAAACGTGATTGCTAGTGAAAATTTAAGAGTTTAGAAGTGTAAATATTGAAATGGAAGGATGAAATGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTACAAGGATGAAATGAAAATTAAACTCATAATTCAAGAAATAACATTTAAGGCCTCATTTGGTAACCATTTGGTTTTTGATTTTTAGTTTTTAAAAAATTAAGCCTACAAACACCCCTCTAACCACGGTTTACCCATGTTTTCAAAATTTCTAAAAAAAAAAAAAGTAGTTTTTAAAAAGTAGTTTTTGTTTTTCGAATTTGGTTAAGAATTCAACTCTTGTATTCAGGAAATACGTAAATCTTGTAAAAAATATACGAGGAAATAGACTTAATTTTCAGAAACCAAAAATAAAAAAACAAAATGGTTACGAAATGGAGTCTAAAGTTTTGAAACTTAGGTTTTGAAGACAAGGTGAAAATATAGGCATCGTTTTGTAACCATTTCGTTTTTGAAAATTAAGCCTACTTCCACCTTCAAATTTATTCATTTGTTATCAACTTTTTACCAATACTATATAAAACCAAGCCAAAAGTTAAAAACTAAAAAAAAAAAGTAGCTTTTAAAAACTTGTTTTTATTTTTGAAATTTGACAAAGAAATGTGAAGAAATAGGCTTAATTTTCAAAAACAAAAACAAAAAAACGAAATGGTTACAAATAGGGCATAATATTTTAAAACTAACCAAGAACTAGACATAAATTCTAGGATCAAATACTATATTTTTCCTTTTTTTTTTCTTCAATTAATAAGTTGGAATATTCTCTAGACATAATATTGATTTTGGGTGTTTTGTTAATTAGTTGGAATTCGTATGAATAATTAATTATTAATTAGCTTAGTAAGAAGTTAATTAATATGGTTTGTTAATTAAATGAAAATGCAGGGTTTAGAGTGGTGGACACAGCGTGCTGTGGAGTAGGTTTAAACAGAGGGCAAATCACATGTTTACCGCTCCAATTTCCATGCCTTAACCGAAACGAGTACGTATTTTGGGATGCCTTTCACCCAACTGAAGCAGCCTCCGCCATTCTTGCCGGCCGAGCTTTCCGAGGTCCGCCCTCTGATTCCTATCCCATCAATGTCCAACAGTTGGCCCTCATCTGA

mRNA sequence

ATGATGACCCAGCGACGTCGTTTCGCCGTGGCCGCCACTTTCGTCGTCGTACTCGTATTTCTGAAATGTTCCAATGAGATGGTTGTAGCGGCAAGTAATGGGGTTTCGGCTATTTTTGTGTTTGGGGACTCTCTTGTGGACGTTGGGAACAATAATTTTCTTCACTCAGCTGCAAGAGCCAATTATTACCCTTATGGGATTGATTTTCATGCTGGTCCTACTGGGAGATTCTCTAATGGACGAACAGTTATAGATATGTTTGTGGACATGCTTGGAATTCCCAACGCTCCAGAATTTGCCAATCCGGACACTTCCGGAGACAGAATATATAACGGTGTCAACTATGCTTCGGCAGCTGCCGGCATACTCGACGAGACCGGCAGACACTATGGAGATAGGTACAGTCTAAGCCAGCAAGTGGTGAATTTTGAAAGCACTTTGGATGAGTTAAGGAGATCAATGGGGAGTTGGAATTTGACTTGTTACTTATCAAAATCCATAGCCTTTTTAGCGTTTGGAAGCAACGATTACATAAACAACTACTTGTTACCGAATCTGTACACTTCCAGATTCAGATACAATTCTAACCAATTCGCCAACTTTCTCCTTAACCGCTATTCTCGACAGCTTCTCGCACTGCAAAGCGTAGGGTTGAGAAAGTTCGTGGTGGCCGGAGTGGGGCCGTTGGGTTGTATCCCGAACCAGAGGGCGACCGGCTTTACCATGCCAGGGCGGTGCGTCGATAATGTGAATGAGATGCTGGGAGCTTTTAATGAAGGGCTGAAATCGTTAGTAACTCAGTTGAATAGCCAGCATCCTGATTCCACCTTCGTGTATACAAATATCTATGGCATTTTTGGCGACATCTTGAACAACCCTGAAACCTACGCCTCCGCCATTCTTGCCGGCCGAGCTTTCCGAGGTCCGCCCTCTGATTCCTATCCCATCAATGTCCAACAGTTGGCCCTCATCTGA

Coding sequence (CDS)

ATGATGACCCAGCGACGTCGTTTCGCCGTGGCCGCCACTTTCGTCGTCGTACTCGTATTTCTGAAATGTTCCAATGAGATGGTTGTAGCGGCAAGTAATGGGGTTTCGGCTATTTTTGTGTTTGGGGACTCTCTTGTGGACGTTGGGAACAATAATTTTCTTCACTCAGCTGCAAGAGCCAATTATTACCCTTATGGGATTGATTTTCATGCTGGTCCTACTGGGAGATTCTCTAATGGACGAACAGTTATAGATATGTTTGTGGACATGCTTGGAATTCCCAACGCTCCAGAATTTGCCAATCCGGACACTTCCGGAGACAGAATATATAACGGTGTCAACTATGCTTCGGCAGCTGCCGGCATACTCGACGAGACCGGCAGACACTATGGAGATAGGTACAGTCTAAGCCAGCAAGTGGTGAATTTTGAAAGCACTTTGGATGAGTTAAGGAGATCAATGGGGAGTTGGAATTTGACTTGTTACTTATCAAAATCCATAGCCTTTTTAGCGTTTGGAAGCAACGATTACATAAACAACTACTTGTTACCGAATCTGTACACTTCCAGATTCAGATACAATTCTAACCAATTCGCCAACTTTCTCCTTAACCGCTATTCTCGACAGCTTCTCGCACTGCAAAGCGTAGGGTTGAGAAAGTTCGTGGTGGCCGGAGTGGGGCCGTTGGGTTGTATCCCGAACCAGAGGGCGACCGGCTTTACCATGCCAGGGCGGTGCGTCGATAATGTGAATGAGATGCTGGGAGCTTTTAATGAAGGGCTGAAATCGTTAGTAACTCAGTTGAATAGCCAGCATCCTGATTCCACCTTCGTGTATACAAATATCTATGGCATTTTTGGCGACATCTTGAACAACCCTGAAACCTACGCCTCCGCCATTCTTGCCGGCCGAGCTTTCCGAGGTCCGCCCTCTGATTCCTATCCCATCAATGTCCAACAGTTGGCCCTCATCTGA

Protein sequence

MMTQRRRFAVAATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETYASAILAGRAFRGPPSDSYPINVQQLALI
Homology
BLAST of HG10021005 vs. NCBI nr
Match: XP_038876998.1 (GDSL esterase/lipase At1g71250 [Benincasa hispida])

HSP 1 Score: 568.9 bits (1465), Expect = 2.7e-158
Identity = 292/369 (79.13%), Postives = 307/369 (83.20%), Query Frame = 0

Query: 1   MMTQRRRFAVAATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARA 60
           M+T++ RFA+A   +VVL+FLKCS EMVVA +  VSA+FVFGDSLVDVGNNNFLHSAARA
Sbjct: 1   MITEQCRFAMATVLIVVLIFLKCSKEMVVAENYRVSAVFVFGDSLVDVGNNNFLHSAARA 60

Query: 61  NYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAA 120
           NYYPYGID+  GPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAA
Sbjct: 61  NYYPYGIDYDGGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAA 120

Query: 121 GILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINN 180
           GILDETGRHYGDRYSLS+QVVNFESTL+ELRRSMGSWNLT YLSKSIAFLAFGSNDYINN
Sbjct: 121 GILDETGRHYGDRYSLSEQVVNFESTLNELRRSMGSWNLTRYLSKSIAFLAFGSNDYINN 180

Query: 181 YLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGF 240
           YLLPNLYTSRFRY S QFAN LLNRY+RQLLALQSVGLRKFVVAGVGPLGCIPNQRATG 
Sbjct: 181 YLLPNLYTSRFRYTSKQFANLLLNRYTRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGV 240

Query: 241 TMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY---- 300
            +PGRCVD+VNEMLGAFNEGL+SLV QLNSQHPDSTFVYTNIYGIFGDILNNPETY    
Sbjct: 241 AIPGRCVDSVNEMLGAFNEGLRSLVIQLNSQHPDSTFVYTNIYGIFGDILNNPETYGFRV 300

Query: 301 -----------------------------------------ASAILAGRAFRGPPSDSYP 325
                                                    ASAILAGRAFRGPPSDSYP
Sbjct: 301 VDTACCGVGLNRGQITCLPLQFPCLNRKEYVFWDAFHPTEAASAILAGRAFRGPPSDSYP 360

BLAST of HG10021005 vs. NCBI nr
Match: XP_008440329.1 (PREDICTED: GDSL esterase/lipase At1g71250 isoform X1 [Cucumis melo] >KAA0055368.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYJ99295.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 558.9 bits (1439), Expect = 2.8e-155
Identity = 288/362 (79.56%), Postives = 299/362 (82.60%), Query Frame = 0

Query: 8   FAVAATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 67
           F  AA  VV+LVFLKCSN+MV A + GV AIFVFGDSLVDVGNNNFLHSAARANYYPYGI
Sbjct: 9   FVAAAAGVVLLVFLKCSNQMVAAENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 68

Query: 68  DFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETG 127
           DF  GPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRI NGVNYASAAAGILDETG
Sbjct: 69  DFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETG 128

Query: 128 RHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLY 187
           RHYGDRYSLSQQVVNFESTL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLY
Sbjct: 129 RHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLY 188

Query: 188 TSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCV 247
           T+RFRYNSNQFAN LLNRYSRQLLAL+SVGLRK VVAGVGPLGCIPNQRATG T+PGRC 
Sbjct: 189 TTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCA 248

Query: 248 DNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY----------- 307
           DNVNEMLGAFNEGLKSLVTQLNSQ+PDS FVYTNIYGIFGDILNNPETY           
Sbjct: 249 DNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCG 308

Query: 308 ----------------------------------ASAILAGRAFRGPPSDSYPINVQQLA 325
                                             AS +LAGRAFRGPPSDSYPINVQQLA
Sbjct: 309 VGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPSDSYPINVQQLA 368

BLAST of HG10021005 vs. NCBI nr
Match: XP_031737721.1 (GDSL esterase/lipase At1g71250 [Cucumis sativus])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 281/359 (78.27%), Postives = 296/359 (82.45%), Query Frame = 0

Query: 11  AATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFH 70
           AA  VVVLVFLK S +M V A NGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYG+DF 
Sbjct: 3   AAAGVVVLVFLKSSYQM-VEAENGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGVDFT 62

Query: 71  AGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHY 130
            GPTGRFSNGRTVIDMFVDMLGIPNAPEF+NPDTSGDRI NGVNYASAAAGILDETGRHY
Sbjct: 63  DGPTGRFSNGRTVIDMFVDMLGIPNAPEFSNPDTSGDRILNGVNYASAAAGILDETGRHY 122

Query: 131 GDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSR 190
           GDRY+LSQQVVNFESTL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLYT+R
Sbjct: 123 GDRYTLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTR 182

Query: 191 FRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNV 250
           FRYNSNQFAN LLNRYSRQLLALQSVG++K V+AG+GPLGCIPNQRATG T+PGRC D V
Sbjct: 183 FRYNSNQFANLLLNRYSRQLLALQSVGVKKLVIAGLGPLGCIPNQRATGVTLPGRCADKV 242

Query: 251 NEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY-------------- 310
           NEMLGAFNEGLKSLVTQLNSQ+PD+ FVYTNIYGIFGDILNNPETY              
Sbjct: 243 NEMLGAFNEGLKSLVTQLNSQYPDTKFVYTNIYGIFGDILNNPETYGFSVVDTACCGVGL 302

Query: 311 -------------------------------ASAILAGRAFRGPPSDSYPINVQQLALI 325
                                          AS ILAGRAFRGPPSDSYPINVQQLALI
Sbjct: 303 NRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASYILAGRAFRGPPSDSYPINVQQLALI 360

BLAST of HG10021005 vs. NCBI nr
Match: XP_016899170.1 (PREDICTED: GDSL esterase/lipase At1g71250 isoform X2 [Cucumis melo])

HSP 1 Score: 533.5 bits (1373), Expect = 1.3e-147
Identity = 272/345 (78.84%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 25  NEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVI 84
           +++ +  + GV AIFVFGDSLVDVGNNNFLHSAARANYYPYGIDF  GPTGRFSNGRTVI
Sbjct: 4   SDIKIKENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFSDGPTGRFSNGRTVI 63

Query: 85  DMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE 144
           DMFVDMLGIPNAPEFANPDTSGDRI NGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE
Sbjct: 64  DMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE 123

Query: 145 STLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLN 204
           STL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLYT+RFRYNSNQFAN LLN
Sbjct: 124 STLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTRFRYNSNQFANLLLN 183

Query: 205 RYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLGAFNEGLKSL 264
           RYSRQLLAL+SVGLRK VVAGVGPLGCIPNQRATG T+PGRC DNVNEMLGAFNEGLKSL
Sbjct: 184 RYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCADNVNEMLGAFNEGLKSL 243

Query: 265 VTQLNSQHPDSTFVYTNIYGIFGDILNNPETY---------------------------- 324
           VTQLNSQ+PDS FVYTNIYGIFGDILNNPETY                            
Sbjct: 244 VTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCGVGLNRGQITCLPLQFPC 303

BLAST of HG10021005 vs. NCBI nr
Match: XP_022137167.1 (GDSL esterase/lipase At1g71250 [Momordica charantia])

HSP 1 Score: 519.2 bits (1336), Expect = 2.5e-143
Identity = 267/373 (71.58%), Postives = 298/373 (79.89%), Query Frame = 0

Query: 1   MMTQRRRF-AVAATFVVVLVFLKCSNEMVVAASN---GVSAIFVFGDSLVDVGNNNFLHS 60
           M+TQRRRF A A+T V+VLV +KCS EMV A      GVS++FVFGDSLVDVGNNNFLHS
Sbjct: 10  MITQRRRFGAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 69

Query: 61  AARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYA 120
           AARAN+YPYG+D+HAGPTGRFSNG+TVIDMFVDML IPNAPEFANPDT+GD+I +GVNYA
Sbjct: 70  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLRIPNAPEFANPDTTGDKILHGVNYA 129

Query: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLSQQVVNFE+T+ +LRR+M S NLT YL KSIAFLAFGSND
Sbjct: 130 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 189

Query: 181 YINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+P LY SR RY + QFAN LLN Y+RQLLALQS+GLRKFVVAGVGPLGCIPNQR
Sbjct: 190 YINNYLMPTLYNSRLRYTAPQFANLLLNHYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 249

Query: 241 ATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 300
           ATG  +PGRCVDNVN+MLG FNEGL+SLV QLN+Q+PDSTFVYTN+YGIFGDILNNP+TY
Sbjct: 250 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 309

Query: 301 ---------------------------------------------ASAILAGRAFRGPPS 325
                                                        ASA+LAGRAFRGPPS
Sbjct: 310 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 369

BLAST of HG10021005 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.0e-96
Identity = 189/373 (50.67%), Postives = 235/373 (63.00%), Query Frame = 0

Query: 1   MMTQRRRFAVAATFVVVLVFLKCS----NEMVVAASNGVSAIFVFGDSLVDVGNNNFLHS 60
           M T R++  V     V+++ L  S       +V     V A+FV GDSLVD GNNNFL +
Sbjct: 1   MNTNRKKMKVHIGGYVLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQT 60

Query: 61  AARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYA 120
            ARAN+ PYGID +  PTGRFSNG T ID+   +L IP+ P FA+P TSG+RI  GVNYA
Sbjct: 61  VARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQGVNYA 120

Query: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSND 180
           SAAAGILD +G +YG R+SL+QQ+VN E+TL +LR  M   N T YL++S+  L FGSND
Sbjct: 121 SAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSND 180

Query: 181 YINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+PNLY S  R+    FAN LL++Y+RQLL L S+GLRK  + GV PLGCIPNQR
Sbjct: 181 YINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQR 240

Query: 241 ATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 300
           A G + P RCVD+VN++LG FN+GLKSLV QLN + P + +VY N Y   GDILNNP  Y
Sbjct: 241 ARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAY 300

Query: 301 ---------------------------------------------ASAILAGRAFRGPPS 325
                                                        A++ILA RAF GPPS
Sbjct: 301 GFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPS 360

BLAST of HG10021005 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.5e-74
Identity = 159/355 (44.79%), Postives = 206/355 (58.03%), Query Frame = 0

Query: 17  VLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAG-PTG 76
           ++VF+    E     +    A+FVFGDSLVD GNNN L+S AR+NY PYGIDF    PTG
Sbjct: 29  LVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTG 88

Query: 77  RFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYS 136
           RFSNG+T++D   ++LG+P  P F +    G  I +GVNYASAA GIL+ETGRH G+R+S
Sbjct: 89  RFSNGKTIVDFIGELLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFS 148

Query: 137 LSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNS 196
           + +QV NFE TL E+ RSM   ++  Y++KS+  ++ G+NDYINNYL P L+ S   Y+ 
Sbjct: 149 MGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDP 208

Query: 197 NQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLG 256
             FA+ LL+ ++  LL L   G RKFV+AGVGPLGCIP+Q A    +PG CV+ VNEM  
Sbjct: 209 TSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAE 268

Query: 257 AFNEGLKSLVTQLNSQH---PDSTFVYTNIYGIFGDILNNPETYA--------------- 316
            FN  L SLV +LNS +    ++ FVY N YG   DIL NP  Y                
Sbjct: 269 LFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNR 328

Query: 317 ------------------------------SAILAGRAFRGPPSDSYPINVQQLA 323
                                         + I+A RAF G  SD YPIN+ QL+
Sbjct: 329 GEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLS 383

BLAST of HG10021005 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 137/334 (41.02%), Postives = 202/334 (60.48%), Query Frame = 0

Query: 35  VSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIP 94
           V A+FVFGDSL+D GNNN + S A+ANY+PYGIDF+ GPTGRF NG T++D    +LG+P
Sbjct: 53  VPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGLP 112

Query: 95  NAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLDEL-RRS 154
             P ++  + +GD++  GVNYASAAAGIL +TG ++  R    QQ+ NFE+TLD++  +S
Sbjct: 113 LIPAYS--EATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKS 172

Query: 155 MGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLAL 214
            G+  +   +++S+ F+  GSNDY+NNYL+PN + +R +YNS QF + L+  Y+ QL  L
Sbjct: 173 GGAVAIADSVTRSLFFIGMGSNDYLNNYLMPN-FPTRNQYNSQQFGDLLVQHYTDQLTRL 232

Query: 215 QSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHP 274
            ++G RKFVVAG+G +GCIP+  A G    G+C + VN+++  FN  +K++++ LN   P
Sbjct: 233 YNLGGRKFVVAGLGRMGCIPSILAQG--NDGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 292

Query: 275 DSTFVYTNIYGIFGDILNNPETY------------------------------------- 323
           D+ F+Y +I  +F DI+ N   Y                                     
Sbjct: 293 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 352

BLAST of HG10021005 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 139/283 (49.12%), Postives = 181/283 (63.96%), Query Frame = 0

Query: 16  VVLVFLKCSNEMVVA-ASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPT 75
           VVLV L     +V A A   V   FVFGDSLVD GNNN L S AR+NY+PYGIDF  GPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 76  GRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRY 135
           GRFSNG+T +D+  ++LG  N    A    SG +I +GVNYASAAAGI +ETGR  G R 
Sbjct: 70  GRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRI 129

Query: 136 SLSQQVVNFESTLDELRRSMGSWNLTC-YLSKSIAFLAFGSNDYINNYLLPNLYTSRFRY 195
           S S QV N+++T+ ++ + +G       YL + I  +  GSNDY+NNY +P  Y+S  ++
Sbjct: 130 SFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQF 189

Query: 196 NSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEM 255
              Q+AN L++RYS QL AL + G RKF ++G+G +GC PN  A G      CVD +N  
Sbjct: 190 TPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA-GSPDGRTCVDRINSA 249

Query: 256 LGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 297
              FN  L+SLV QLN+ HPD+ F+Y N YGIF D++ NP  +
Sbjct: 250 NQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARF 289

BLAST of HG10021005 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.8e-64
Identity = 133/268 (49.63%), Postives = 171/268 (63.81%), Query Frame = 0

Query: 37  AIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNA 96
           A FVFGDSLVD GNNN++ + ARANY+PYGIDF   PTGRF NGRTV+D     LG+P  
Sbjct: 30  AFFVFGDSLVDSGNNNYIPTLARANYFPYGIDF-GFPTGRFCNGRTVVDYGATYLGLPLV 89

Query: 97  PEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLD-ELRR-SM 156
           P + +P + G     GVNYASAAAGILDETGRHYG R + + Q+  FE T++  LRR   
Sbjct: 90  PPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQ 149

Query: 157 GSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQ 216
              +L  YL+KSI  +  GSNDYINNYL+P  Y++   Y+   +A+ L+   S Q+  L 
Sbjct: 150 NPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLY 209

Query: 217 SVGLRKFVVAGVGPLGCIPNQ--RATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQH 276
           ++G RK V+AG GPLGCIP+Q    TG    G CV  +N M+  FN  LK L   LN+  
Sbjct: 210 NLGARKMVLAGSGPLGCIPSQLSMVTGNNTSG-CVTKINNMVSMFNSRLKDLANTLNTTL 269

Query: 277 PDSTFVYTNIYGIFGDILNNPETYASAI 301
           P S FVY N++ +F D++ NP  Y   +
Sbjct: 270 PGSFFVYQNVFDLFHDMVVNPSRYGLVV 295

BLAST of HG10021005 vs. ExPASy TrEMBL
Match: A0A5A7UK29 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005080 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 288/362 (79.56%), Postives = 299/362 (82.60%), Query Frame = 0

Query: 8   FAVAATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 67
           F  AA  VV+LVFLKCSN+MV A + GV AIFVFGDSLVDVGNNNFLHSAARANYYPYGI
Sbjct: 9   FVAAAAGVVLLVFLKCSNQMVAAENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 68

Query: 68  DFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETG 127
           DF  GPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRI NGVNYASAAAGILDETG
Sbjct: 69  DFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETG 128

Query: 128 RHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLY 187
           RHYGDRYSLSQQVVNFESTL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLY
Sbjct: 129 RHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLY 188

Query: 188 TSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCV 247
           T+RFRYNSNQFAN LLNRYSRQLLAL+SVGLRK VVAGVGPLGCIPNQRATG T+PGRC 
Sbjct: 189 TTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCA 248

Query: 248 DNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY----------- 307
           DNVNEMLGAFNEGLKSLVTQLNSQ+PDS FVYTNIYGIFGDILNNPETY           
Sbjct: 249 DNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCG 308

Query: 308 ----------------------------------ASAILAGRAFRGPPSDSYPINVQQLA 325
                                             AS +LAGRAFRGPPSDSYPINVQQLA
Sbjct: 309 VGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPSDSYPINVQQLA 368

BLAST of HG10021005 vs. ExPASy TrEMBL
Match: A0A1S3B1L6 (GDSL esterase/lipase At1g71250 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484808 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 288/362 (79.56%), Postives = 299/362 (82.60%), Query Frame = 0

Query: 8   FAVAATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 67
           F  AA  VV+LVFLKCSN+MV A + GV AIFVFGDSLVDVGNNNFLHSAARANYYPYGI
Sbjct: 9   FVAAAAGVVLLVFLKCSNQMVAAENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGI 68

Query: 68  DFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETG 127
           DF  GPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRI NGVNYASAAAGILDETG
Sbjct: 69  DFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETG 128

Query: 128 RHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLY 187
           RHYGDRYSLSQQVVNFESTL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLY
Sbjct: 129 RHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLY 188

Query: 188 TSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCV 247
           T+RFRYNSNQFAN LLNRYSRQLLAL+SVGLRK VVAGVGPLGCIPNQRATG T+PGRC 
Sbjct: 189 TTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCA 248

Query: 248 DNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY----------- 307
           DNVNEMLGAFNEGLKSLVTQLNSQ+PDS FVYTNIYGIFGDILNNPETY           
Sbjct: 249 DNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCG 308

Query: 308 ----------------------------------ASAILAGRAFRGPPSDSYPINVQQLA 325
                                             AS +LAGRAFRGPPSDSYPINVQQLA
Sbjct: 309 VGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPSDSYPINVQQLA 368

BLAST of HG10021005 vs. ExPASy TrEMBL
Match: A0A0A0LUJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G056930 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.8e-151
Identity = 281/359 (78.27%), Postives = 296/359 (82.45%), Query Frame = 0

Query: 11  AATFVVVLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFH 70
           AA  VVVLVFLK S +M V A NGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYG+DF 
Sbjct: 3   AAAGVVVLVFLKSSYQM-VEAENGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGVDFT 62

Query: 71  AGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHY 130
            GPTGRFSNGRTVIDMFVDMLGIPNAPEF+NPDTSGDRI NGVNYASAAAGILDETGRHY
Sbjct: 63  DGPTGRFSNGRTVIDMFVDMLGIPNAPEFSNPDTSGDRILNGVNYASAAAGILDETGRHY 122

Query: 131 GDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSR 190
           GDRY+LSQQVVNFESTL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLYT+R
Sbjct: 123 GDRYTLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTR 182

Query: 191 FRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNV 250
           FRYNSNQFAN LLNRYSRQLLALQSVG++K V+AG+GPLGCIPNQRATG T+PGRC D V
Sbjct: 183 FRYNSNQFANLLLNRYSRQLLALQSVGVKKLVIAGLGPLGCIPNQRATGVTLPGRCADKV 242

Query: 251 NEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY-------------- 310
           NEMLGAFNEGLKSLVTQLNSQ+PD+ FVYTNIYGIFGDILNNPETY              
Sbjct: 243 NEMLGAFNEGLKSLVTQLNSQYPDTKFVYTNIYGIFGDILNNPETYGFSVVDTACCGVGL 302

Query: 311 -------------------------------ASAILAGRAFRGPPSDSYPINVQQLALI 325
                                          AS ILAGRAFRGPPSDSYPINVQQLALI
Sbjct: 303 NRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASYILAGRAFRGPPSDSYPINVQQLALI 360

BLAST of HG10021005 vs. ExPASy TrEMBL
Match: A0A1S4DTZ1 (GDSL esterase/lipase At1g71250 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484808 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.2e-148
Identity = 272/345 (78.84%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 25  NEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVI 84
           +++ +  + GV AIFVFGDSLVDVGNNNFLHSAARANYYPYGIDF  GPTGRFSNGRTVI
Sbjct: 4   SDIKIKENYGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFSDGPTGRFSNGRTVI 63

Query: 85  DMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE 144
           DMFVDMLGIPNAPEFANPDTSGDRI NGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE
Sbjct: 64  DMFVDMLGIPNAPEFANPDTSGDRILNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFE 123

Query: 145 STLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLN 204
           STL++LRRSMGSWNLT YLSKSIAFLAFGSNDYINNYL+PNLYT+RFRYNSNQFAN LLN
Sbjct: 124 STLNDLRRSMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTRFRYNSNQFANLLLN 183

Query: 205 RYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLGAFNEGLKSL 264
           RYSRQLLAL+SVGLRK VVAGVGPLGCIPNQRATG T+PGRC DNVNEMLGAFNEGLKSL
Sbjct: 184 RYSRQLLALRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCADNVNEMLGAFNEGLKSL 243

Query: 265 VTQLNSQHPDSTFVYTNIYGIFGDILNNPETY---------------------------- 324
           VTQLNSQ+PDS FVYTNIYGIFGDILNNPETY                            
Sbjct: 244 VTQLNSQYPDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCGVGLNRGQITCLPLQFPC 303

BLAST of HG10021005 vs. ExPASy TrEMBL
Match: A0A6J1C7I1 (GDSL esterase/lipase At1g71250 OS=Momordica charantia OX=3673 GN=LOC111008710 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 267/373 (71.58%), Postives = 298/373 (79.89%), Query Frame = 0

Query: 1   MMTQRRRF-AVAATFVVVLVFLKCSNEMVVAASN---GVSAIFVFGDSLVDVGNNNFLHS 60
           M+TQRRRF A A+T V+VLV +KCS EMV A      GVS++FVFGDSLVDVGNNNFLHS
Sbjct: 10  MITQRRRFGAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 69

Query: 61  AARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYA 120
           AARAN+YPYG+D+HAGPTGRFSNG+TVIDMFVDML IPNAPEFANPDT+GD+I +GVNYA
Sbjct: 70  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLRIPNAPEFANPDTTGDKILHGVNYA 129

Query: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLSQQVVNFE+T+ +LRR+M S NLT YL KSIAFLAFGSND
Sbjct: 130 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 189

Query: 181 YINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+P LY SR RY + QFAN LLN Y+RQLLALQS+GLRKFVVAGVGPLGCIPNQR
Sbjct: 190 YINNYLMPTLYNSRLRYTAPQFANLLLNHYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 249

Query: 241 ATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 300
           ATG  +PGRCVDNVN+MLG FNEGL+SLV QLN+Q+PDSTFVYTN+YGIFGDILNNP+TY
Sbjct: 250 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 309

Query: 301 ---------------------------------------------ASAILAGRAFRGPPS 325
                                                        ASA+LAGRAFRGPPS
Sbjct: 310 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 369

BLAST of HG10021005 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 351.7 bits (901), Expect = 6.4e-97
Identity = 189/373 (50.67%), Postives = 235/373 (63.00%), Query Frame = 0

Query: 1   MMTQRRRFAVAATFVVVLVFLKCS----NEMVVAASNGVSAIFVFGDSLVDVGNNNFLHS 60
           M T R++  V     V+++ L  S       +V     V A+FV GDSLVD GNNNFL +
Sbjct: 1   MNTNRKKMKVHIGGYVLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQT 60

Query: 61  AARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYA 120
            ARAN+ PYGID +  PTGRFSNG T ID+   +L IP+ P FA+P TSG+RI  GVNYA
Sbjct: 61  VARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQGVNYA 120

Query: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSND 180
           SAAAGILD +G +YG R+SL+QQ+VN E+TL +LR  M   N T YL++S+  L FGSND
Sbjct: 121 SAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSND 180

Query: 181 YINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+PNLY S  R+    FAN LL++Y+RQLL L S+GLRK  + GV PLGCIPNQR
Sbjct: 181 YINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQR 240

Query: 241 ATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 300
           A G + P RCVD+VN++LG FN+GLKSLV QLN + P + +VY N Y   GDILNNP  Y
Sbjct: 241 ARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAY 300

Query: 301 ---------------------------------------------ASAILAGRAFRGPPS 325
                                                        A++ILA RAF GPPS
Sbjct: 301 GFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPS 360

BLAST of HG10021005 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.1e-75
Identity = 159/355 (44.79%), Postives = 206/355 (58.03%), Query Frame = 0

Query: 17  VLVFLKCSNEMVVAASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAG-PTG 76
           ++VF+    E     +    A+FVFGDSLVD GNNN L+S AR+NY PYGIDF    PTG
Sbjct: 29  LVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTG 88

Query: 77  RFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYS 136
           RFSNG+T++D   ++LG+P  P F +    G  I +GVNYASAA GIL+ETGRH G+R+S
Sbjct: 89  RFSNGKTIVDFIGELLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFS 148

Query: 137 LSQQVVNFESTLDELRRSMGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNS 196
           + +QV NFE TL E+ RSM   ++  Y++KS+  ++ G+NDYINNYL P L+ S   Y+ 
Sbjct: 149 MGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDP 208

Query: 197 NQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLG 256
             FA+ LL+ ++  LL L   G RKFV+AGVGPLGCIP+Q A    +PG CV+ VNEM  
Sbjct: 209 TSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAE 268

Query: 257 AFNEGLKSLVTQLNSQH---PDSTFVYTNIYGIFGDILNNPETYA--------------- 316
            FN  L SLV +LNS +    ++ FVY N YG   DIL NP  Y                
Sbjct: 269 LFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNR 328

Query: 317 ------------------------------SAILAGRAFRGPPSDSYPINVQQLA 323
                                         + I+A RAF G  SD YPIN+ QL+
Sbjct: 329 GEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLS 383

BLAST of HG10021005 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 9.0e-67
Identity = 137/334 (41.02%), Postives = 202/334 (60.48%), Query Frame = 0

Query: 35  VSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIP 94
           V A+FVFGDSL+D GNNN + S A+ANY+PYGIDF+ GPTGRF NG T++D    +LG+P
Sbjct: 53  VPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLGLP 112

Query: 95  NAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLDEL-RRS 154
             P ++  + +GD++  GVNYASAAAGIL +TG ++  R    QQ+ NFE+TLD++  +S
Sbjct: 113 LIPAYS--EATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKS 172

Query: 155 MGSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLAL 214
            G+  +   +++S+ F+  GSNDY+NNYL+PN + +R +YNS QF + L+  Y+ QL  L
Sbjct: 173 GGAVAIADSVTRSLFFIGMGSNDYLNNYLMPN-FPTRNQYNSQQFGDLLVQHYTDQLTRL 232

Query: 215 QSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQHP 274
            ++G RKFVVAG+G +GCIP+  A G    G+C + VN+++  FN  +K++++ LN   P
Sbjct: 233 YNLGGRKFVVAGLGRMGCIPSILAQG--NDGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 292

Query: 275 DSTFVYTNIYGIFGDILNNPETY------------------------------------- 323
           D+ F+Y +I  +F DI+ N   Y                                     
Sbjct: 293 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 352

BLAST of HG10021005 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-65
Identity = 139/283 (49.12%), Postives = 181/283 (63.96%), Query Frame = 0

Query: 16  VVLVFLKCSNEMVVA-ASNGVSAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPT 75
           VVLV L     +V A A   V   FVFGDSLVD GNNN L S AR+NY+PYGIDF  GPT
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GGPT 69

Query: 76  GRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRY 135
           GRFSNG+T +D+  ++LG  N    A    SG +I +GVNYASAAAGI +ETGR  G R 
Sbjct: 70  GRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRI 129

Query: 136 SLSQQVVNFESTLDELRRSMGSWNLTC-YLSKSIAFLAFGSNDYINNYLLPNLYTSRFRY 195
           S S QV N+++T+ ++ + +G       YL + I  +  GSNDY+NNY +P  Y+S  ++
Sbjct: 130 SFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQF 189

Query: 196 NSNQFANFLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQRATGFTMPGRCVDNVNEM 255
              Q+AN L++RYS QL AL + G RKF ++G+G +GC PN  A G      CVD +N  
Sbjct: 190 TPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA-GSPDGRTCVDRINSA 249

Query: 256 LGAFNEGLKSLVTQLNSQHPDSTFVYTNIYGIFGDILNNPETY 297
              FN  L+SLV QLN+ HPD+ F+Y N YGIF D++ NP  +
Sbjct: 250 NQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARF 289

BLAST of HG10021005 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 247.7 bits (631), Expect = 1.3e-65
Identity = 133/268 (49.63%), Postives = 171/268 (63.81%), Query Frame = 0

Query: 37  AIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFHAGPTGRFSNGRTVIDMFVDMLGIPNA 96
           A FVFGDSLVD GNNN++ + ARANY+PYGIDF   PTGRF NGRTV+D     LG+P  
Sbjct: 30  AFFVFGDSLVDSGNNNYIPTLARANYFPYGIDF-GFPTGRFCNGRTVVDYGATYLGLPLV 89

Query: 97  PEFANPDTSGDRIYNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLD-ELRR-SM 156
           P + +P + G     GVNYASAAAGILDETGRHYG R + + Q+  FE T++  LRR   
Sbjct: 90  PPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQ 149

Query: 157 GSWNLTCYLSKSIAFLAFGSNDYINNYLLPNLYTSRFRYNSNQFANFLLNRYSRQLLALQ 216
              +L  YL+KSI  +  GSNDYINNYL+P  Y++   Y+   +A+ L+   S Q+  L 
Sbjct: 150 NPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLY 209

Query: 217 SVGLRKFVVAGVGPLGCIPNQ--RATGFTMPGRCVDNVNEMLGAFNEGLKSLVTQLNSQH 276
           ++G RK V+AG GPLGCIP+Q    TG    G CV  +N M+  FN  LK L   LN+  
Sbjct: 210 NLGARKMVLAGSGPLGCIPSQLSMVTGNNTSG-CVTKINNMVSMFNSRLKDLANTLNTTL 269

Query: 277 PDSTFVYTNIYGIFGDILNNPETYASAI 301
           P S FVY N++ +F D++ NP  Y   +
Sbjct: 270 PGSFFVYQNVFDLFHDMVVNPSRYGLVV 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876998.12.7e-15879.13GDSL esterase/lipase At1g71250 [Benincasa hispida][more]
XP_008440329.12.8e-15579.56PREDICTED: GDSL esterase/lipase At1g71250 isoform X1 [Cucumis melo] >KAA0055368.... [more]
XP_031737721.11.6e-15078.27GDSL esterase/lipase At1g71250 [Cucumis sativus][more]
XP_016899170.11.3e-14778.84PREDICTED: GDSL esterase/lipase At1g71250 isoform X2 [Cucumis melo][more]
XP_022137167.12.5e-14371.58GDSL esterase/lipase At1g71250 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9FVV19.0e-9650.67GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2... [more]
Q9FNP21.5e-7444.79GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2... [more]
Q9SF781.3e-6541.02GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2... [more]
Q9C7N41.4e-6449.12GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2... [more]
Q8LFJ91.8e-6449.63GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UK291.4e-15579.56GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248... [more]
A0A1S3B1L61.4e-15579.56GDSL esterase/lipase At1g71250 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348480... [more]
A0A0A0LUJ97.8e-15178.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G056930 PE=3 SV=1[more]
A0A1S4DTZ16.2e-14878.84GDSL esterase/lipase At1g71250 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348480... [more]
A0A6J1C7I11.2e-14371.58GDSL esterase/lipase At1g71250 OS=Momordica charantia OX=3673 GN=LOC111008710 PE... [more]
Match NameE-valueIdentityDescription
AT1G71250.16.4e-9750.67GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G08460.11.1e-7544.79GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G71691.29.0e-6741.02GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G29670.11.0e-6549.12GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G15720.11.3e-6549.63GDSL-motif lipase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 38..289
e-value: 8.2E-25
score: 88.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 23..317
e-value: 5.5E-56
score: 192.3
NoneNo IPR availablePANTHERPTHR45650GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATEDcoord: 11..297
NoneNo IPR availablePANTHERPTHR45650:SF32GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 11..297
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 37..296
e-value: 2.32806E-98
score: 291.056

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10021005.1HG10021005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds