HG10020950 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020950
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Pectinesterase
Location	Chr05: 3907697 .. 3908813 (-)
RNA-Seq Expression	HG10020950
Synteny	HG10020950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGGTAACAACTTTCACACTTTTTGTTTGTATTGCTAGCTTTTAAATATTAACTTTGCATTTTGATATGAAGTTACCCAAAAAAACTTTTTCTTTTGCAGTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTCGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

mRNA sequence

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTCGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

Coding sequence (CDS)

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTCGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

Protein sequence

MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL

Homology

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BLAST of HG10020950 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-107
Identity = 195/321 (60.75%), Postives = 246/321 (76.64%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALF 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SAT  V+G+NFMA+S+ FENSAGP+NGQAVA+F
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 NDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG 198
            D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+G+ M+QDC IY R+ 
Sbjct: 121 -DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMP 180

Query: 199 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 258
           + + T+TAQSK +L   SGF FQNC+VT SPE+   K  N +V+LGRPWR YS V+FM+S
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQ-NVKVFLGRPWRQYSNVVFMES 240

Query: 259 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 318
           +LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H+++  + AI +TV
Sbjct: 241 FLDDVVASEGWMEWKGV-PVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKS-AIGFTV 300

Query: 319 NLFIDGNRWLPETGVPFRSTL 340
           + FIDG+ WL ETG+PFR  L
Sbjct: 301 DNFIDGSEWLAETGIPFRRGL 317

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BLAST of HG10020950 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.8 bits (1026), Expect = 2.3e-107
Identity = 208/342 (60.82%), Postives = 251/342 (73.39%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIH 60
           M+ FFI F     FV         IS+N  VSLDG  ++ +IGAAIAAA +N++SRF IH
Sbjct: 1   MSCFFILF-----FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIH 60

Query: 61  VKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQS 120
           VKPG+Y E+++IE  KTFI L GDDA+TT IV +RS ATGF T+ SATFVV  N+F+A S
Sbjct: 61  VKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVS 120

Query: 121 IMFENS--AGPENGQAVALFN-DHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTV 180
           I F NS  AGP++GQAVAL N ++ N TV+YKCVFLG+QDTL++NG  +FFKD EIYGTV
Sbjct: 121 ITFSNSAGAGPKHGQAVALLNSNNENYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTV 180

Query: 181 DFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNL 240
           DFIFG    ++QDC+IY RL N T T+TAQSK+Y  Q SGF FQNCSV ASPEL      
Sbjct: 181 DFIFGNALVVFQDCRIYARLLNNTVTVTAQSKEYKRQLSGFSFQNCSVMASPELGPVNET 240

Query: 241 NFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTR 300
              V+LGRPWRDYSTVIFM+S+LD VVNPAGW+ WS    L LLFYAEY NRGPGA+L+ 
Sbjct: 241 --IVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSG 300

Query: 301 RVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           RVKW GFH I +  +A  ++V  FI+G  WLPETGVP+R  L
Sbjct: 301 RVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

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BLAST of HG10020950 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 395.2 bits (1014), Expect = 5.7e-106
Identity = 207/342 (60.53%), Postives = 248/342 (72.51%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIH 60
           M+ FFI F     FV         IS+N  VSLDG  ++ +IGAAIAAA +N++SRF IH
Sbjct: 1   MSCFFILF-----FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIH 60

Query: 61  VKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQS 120
           VKPG+Y E++ IE  KTFI L GDDA+TT IV +RS ATGF T+ SATFVV  N+F+A S
Sbjct: 61  VKPGMYFEYIRIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVS 120

Query: 121 IMFENS--AGPENGQAVALFN-DHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTV 180
           I F NS  AGP +GQAVAL N ++ N TV+YKCVFLG+QDTL++NG  +FFKD EIYGTV
Sbjct: 121 ITFSNSAGAGPNHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTV 180

Query: 181 DFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNL 240
           DFIFG    ++QDC+IY RL N T T+TAQSK+   Q SGF FQNCSV ASPEL      
Sbjct: 181 DFIFGNALVVFQDCRIYARLLNNTVTVTAQSKENQLQLSGFSFQNCSVMASPELGPVNET 240

Query: 241 NFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTR 300
              V+LGRPWRDYSTVIFM+S+LD VVNPAGW+ WS    L LLFYAEY NRGPGA+L+ 
Sbjct: 241 --IVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSG 300

Query: 301 RVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           RVKW GFH I +  +A  ++V  FI+G  WLPETGVP+R  L
Sbjct: 301 RVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

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BLAST of HG10020950 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 391.7 bits (1005), Expect = 6.3e-105
Identity = 206/341 (60.41%), Postives = 241/341 (70.67%), Query Frame = 0


Query: 3   MFFIKFVAVCLFVLSCMDFV----HSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 62
           M   +FVAV    LSCM+F      +  FN  VSLDG+ ++K+I  AIAAA  N+ +RFY
Sbjct: 1   MVVFQFVAV-FVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFY 60

Query: 63  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMA 122
           IHV  G Y EHVEI   KTF+ALIGDDA+TT+IVD+RS+  GF+T  SATF VKG NFMA
Sbjct: 61  IHVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMA 120

Query: 123 QSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVD 182
           +SI F+NSAGPENGQAVAL ND A+ TVYY C+FLGFQDTLYV GD              
Sbjct: 121 KSITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD-------------- 180

Query: 183 FIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLN 242
                     +DCKIYV L N T TITAQSK+   + SGF FQNCSVT SPEL   K+ N
Sbjct: 181 ----------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQN 240

Query: 243 FEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRR 302
           F+VYLGRP RDYSTVIFM S+LD VVNP GW  WS   PLNLLFYAE+NNRGPGA +++R
Sbjct: 241 FDVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKR 300

Query: 303 VKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           +KW G+H+IN+P +A  YTV  FIDG  WLPET VPFR +L
Sbjct: 301 IKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

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BLAST of HG10020950 vs. NCBI nr
Match: TYJ99208.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 391.3 bits (1004), Expect = 8.2e-105
Identity = 192/321 (59.81%), Postives = 242/321 (75.39%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALF 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SAT  V+GNNFMA+S+ FENSAGP+NGQAVA+F
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 NDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG 198
            D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+GQ M+QDC IY R+ 
Sbjct: 121 -DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMP 180

Query: 199 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 258
           + + T+TAQSK +L   SGF FQN +VT  PE+   K  N +V+LGR WR YS V+FM+S
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQ-NVKVFLGRLWRQYSNVVFMES 240

Query: 259 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 318
           +LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H++N  + A  +TV
Sbjct: 241 FLDDVVASEGWMEWKGV-PINNLFYGEFNNYGPGADVSKRVNWTGYHILNKES-AFRFTV 300

Query: 319 NLFIDGNRWLPETGVPFRSTL 340
           + FI+ ++WL ETG+PFR  L
Sbjct: 301 DNFINRSKWLHETGIPFRHGL 317

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BLAST of HG10020950 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 142/313 (45.37%), Postives = 205/313 (65.50%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           N +V+ DG+ N K+I  A+AAAS    SR+ I++K G Y E++E++ +   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 88  TTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVY 147
            T+I  S+S   G  T +SAT  V G+NF+A+ I   N+AGP N QAVAL    ++ +V+
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVAL-RSGSDLSVF 321

Query: 148 YKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRL-GNRTTTITA 207
           Y+C F G+QDTLYV+    F+++ +IYGTVDFIFG    + Q+C I+ R   NRT T+TA
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTA 381

Query: 208 QSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNP 267
           Q +   +Q +G +  NC VTA+ +LK  ++ + + +LGRPW+ YS  +++ ++LD ++NP
Sbjct: 382 QGRTDPNQSTGIIIHNCRVTAASDLKPVQS-SVKTFLGRPWKQYSRTVYIKTFLDSLINP 441

Query: 268 AGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNR 327
           AGW  WS +  LN L+YAEY N GPG+S   RVKW G+HV+ SP++   +TV  FI GN 
Sbjct: 442 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 501

Query: 328 WLPETGVPFRSTL 340
           WLP T VPF S L
Sbjct: 502 WLPATNVPFTSGL 510

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BLAST of HG10020950 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 139/310 (44.84%), Postives = 197/310 (63.55%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           + +V+ DGT NF +I  A+ AA   +  R+ IHVK G+Y E+VEI+K+K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 88  TTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVY 147
            TVI  +RS   G+ T +SATF V G  F+A+ I F+N+AGPE  QAVA+ +D  +  V+
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSD-TDLGVF 333

Query: 148 YKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG--NRTTTIT 207
           Y+C   G+QDTLY +    FF++  I GTVDFIFG   A++Q C+I  + G  N+  +IT
Sbjct: 334 YRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSIT 393

Query: 208 AQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVN 267
           AQ ++  ++ +GF  Q  ++ A  +L    N     YLGRPW+ YS  +FM +Y+   +N
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT-ATYLGRPWKLYSRTVFMQNYMSDAIN 453

Query: 268 PAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGN 327
           P GW  W+    L+ L+Y EY N GPGASL RRVKW G+HV+N+ A+A  +TV+  I GN
Sbjct: 454 PVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGN 513

Query: 328 RWLPETGVPF 336
            WLP TG+ F
Sbjct: 514 LWLPSTGITF 521

[Close]

BLAST of HG10020950 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 139/313 (44.41%), Postives = 199/313 (63.58%), Query Frame = 0


Query: 25  ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGD 84
           +S + IV+ DGT NF ++  A+ AA   +  R+ I++K G YKE+VEI+K+K  + +IGD
Sbjct: 205 VSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGD 264

Query: 85  DAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANC 144
               T+I  +RS   G+ T +SATF V G  F+A+ I FEN+AGPE  QAVAL +D ++ 
Sbjct: 265 GMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSD-SDL 324

Query: 145 TVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG--NRTT 204
           +V+Y+C   G+QDTLY +    F++D +I GTVDFIFG    ++Q+C+I  + G  N+  
Sbjct: 325 SVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKN 384

Query: 205 TITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDY 264
           +ITAQ ++  ++ +G   Q C++TA  +L+     +   YLGRPW+ YS  + M S+L  
Sbjct: 385 SITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSN 444

Query: 265 VVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFI 324
           V+ P GW  W+ +  LN LFY EY N GPGA L  RVKW G+ V N   +A  YTV  FI
Sbjct: 445 VIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFI 504

Query: 325 DGNRWLPETGVPF 336
           +GN WLP TGV +
Sbjct: 505 EGNLWLPSTGVKY 516

[Close]

BLAST of HG10020950 vs. ExPASy Swiss-Prot
Match: Q9M9W7 (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-72
Identity = 137/314 (43.63%), Postives = 199/314 (63.38%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           NT+V++DG   +++I  AI  A +++  R+ I+VK G+YKE+++++K+KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 88  TTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVY 147
            T+I   R+   G  T ++AT  V G  F+A+ I F N+AGP+N QAVAL  D ++ + +
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVD-SDQSAF 359

Query: 148 YKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVR--LGNRTTTIT 207
           Y+C   G+QDTLY +    F++D EIYGT+DFIFG G A+ Q+CKIY R  L  +  TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 208 AQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVN 267
           AQ ++  +Q +GFV QN  V A+             YLGRPW+ YS  ++M++Y+  +V 
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNTYMSQLVQ 479

Query: 268 PAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGN 327
           P GW  W     L+ L+Y EYNN GPG   + RVKW G+H+++    A+ +TV  FIDG 
Sbjct: 480 PRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK-RTALSFTVGSFIDGR 539

Query: 328 RWLPETGVPFRSTL 340
           RWLP TGV F + L
Sbjct: 540 RWLPATGVTFTAGL 541

[Close]

BLAST of HG10020950 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-72
Identity = 140/316 (44.30%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 26  SFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDD 85
           +++  V+LDGT NF  I  AI  A   +++RF I++K G+Y E+VEI+K+K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 86  AATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCT 145
              TVI  +RS   G+ T +SATF V G  F+A+ I F+N+AGPE  QAVAL +D ++ +
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSD-SDLS 329

Query: 146 VYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG--NRTTT 205
           V+++C   G+QDTLY +    F+++  I GTVDFIFG G  ++Q+C+I  + G  N+  T
Sbjct: 330 VFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNT 389

Query: 206 ITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYV 265
           ITAQ ++ ++Q SGF  Q  +++A  +L    N     YLGRPW+ YS  +F+ + +  V
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTT-RTYLGRPWKLYSRTVFIRNNMSDV 449

Query: 266 VNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFID 325
           V P GW  W+A+  L+ LFY E+ N GPG+ L+ RVKW G+HV N+  +A  +TV+ FI 
Sbjct: 450 VRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIK 509

Query: 326 GNRWLPETGVPFRSTL 340
           GN WLP TGV F   L
Sbjct: 510 GNLWLPSTGVTFSDGL 523

[Close]

BLAST of HG10020950 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 6.5e-108
Identity = 195/321 (60.75%), Postives = 246/321 (76.64%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALF 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SAT  V+G+NFMA+S+ FENSAGP+NGQAVA+F
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 NDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG 198
            D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+G+ M+QDC IY R+ 
Sbjct: 121 -DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMP 180

Query: 199 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 258
           + + T+TAQSK +L   SGF FQNC+VT SPE+   K  N +V+LGRPWR YS V+FM+S
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQ-NVKVFLGRPWRQYSNVVFMES 240

Query: 259 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 318
           +LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H+++  + AI +TV
Sbjct: 241 FLDDVVASEGWMEWKGV-PVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKS-AIGFTV 300

Query: 319 NLFIDGNRWLPETGVPFRSTL 340
           + FIDG+ WL ETG+PFR  L
Sbjct: 301 DNFIDGSEWLAETGIPFRRGL 317

[Close]

BLAST of HG10020950 vs. ExPASy TrEMBL
Match: A0A6J1C9Z6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 3.0e-105
Identity = 206/341 (60.41%), Postives = 241/341 (70.67%), Query Frame = 0


Query: 3   MFFIKFVAVCLFVLSCMDFV----HSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 62
           M   +FVAV    LSCM+F      +  FN  VSLDG+ ++K+I  AIAAA  N+ +RFY
Sbjct: 1   MVVFQFVAV-FVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFY 60

Query: 63  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMA 122
           IHV  G Y EHVEI   KTF+ALIGDDA+TT+IVD+RS+  GF+T  SATF VKG NFMA
Sbjct: 61  IHVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMA 120

Query: 123 QSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVD 182
           +SI F+NSAGPENGQAVAL ND A+ TVYY C+FLGFQDTLYV GD              
Sbjct: 121 KSITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD-------------- 180

Query: 183 FIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLN 242
                     +DCKIYV L N T TITAQSK+   + SGF FQNCSVT SPEL   K+ N
Sbjct: 181 ----------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQN 240

Query: 243 FEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRR 302
           F+VYLGRP RDYSTVIFM S+LD VVNP GW  WS   PLNLLFYAE+NNRGPGA +++R
Sbjct: 241 FDVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKR 300

Query: 303 VKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           +KW G+H+IN+P +A  YTV  FIDG  WLPET VPFR +L
Sbjct: 301 IKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of HG10020950 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 4.0e-105
Identity = 192/321 (59.81%), Postives = 242/321 (75.39%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALF 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SAT  V+GNNFMA+S+ FENSAGP+NGQAVA+F
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 NDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG 198
            D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+GQ M+QDC IY R+ 
Sbjct: 121 -DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMP 180

Query: 199 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 258
           + + T+TAQSK +L   SGF FQN +VT  PE+   K  N +V+LGR WR YS V+FM+S
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQ-NVKVFLGRLWRQYSNVVFMES 240

Query: 259 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 318
           +LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H++N  + A  +TV
Sbjct: 241 FLDDVVASEGWMEWKGV-PINNLFYGEFNNYGPGADVSKRVNWTGYHILNKES-AFRFTV 300

Query: 319 NLFIDGNRWLPETGVPFRSTL 340
           + FI+ ++WL ETG+PFR  L
Sbjct: 301 DNFINRSKWLHETGIPFRHGL 317

[Close]

BLAST of HG10020950 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 2.0e-104
Identity = 193/333 (57.96%), Postives = 249/333 (74.77%), Query Frame = 0


Query: 8   FVAVCLFVLSCMDFVHS-ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIY 67
           F+ +   +L  ++F H  I+FN  VSLDG+ ++  I  AIAAA +N + RFYIHVKPGIY
Sbjct: 9   FMWLVFLLLHSVEFSHCLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKPGIY 68

Query: 68  KEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENS 127
           KEH+E+ + KTFI+LIGDDA+TTVIVD RS+  GF T+ SATF VK  +F+AQ + F+NS
Sbjct: 69  KEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTFQNS 128

Query: 128 AGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQA 187
           AGPENGQAVA+ ++ AN T YYKCVF+G+QDTLYV G    FK+ ++YG++DFIFG G A
Sbjct: 129 AGPENGQAVAVLSE-ANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGLA 188

Query: 188 MYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRP 247
           ++QDC +Y R+    TTITAQSK Y DQRSGF FQNC+VT SPE++  K+    V LGRP
Sbjct: 189 IFQDCNVYARI--LGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKD-QVTVSLGRP 248

Query: 248 WRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHV 307
           WR+YSTVIFM+S+LD +V P GW+ W    P+ LLFYAEYNN G GA  ++RVKW G+HV
Sbjct: 249 WRNYSTVIFMESFLDDIVEPGGWSEW-PRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHV 308

Query: 308 INSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           +++  +A  +TV +FIDG  WL ETG+PFR  L
Sbjct: 309 LHNEDEASRFTVEIFIDGLEWLSETGIPFRRGL 336

[Close]

BLAST of HG10020950 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.4e-102
Identity = 194/341 (56.89%), Postives = 241/341 (70.67%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVL--SCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 60
           MT   + F  V L V+    ++F +S +FN  VSLDG  NF  +  AIAAA + + SRFY
Sbjct: 1   MTFKVVMFFIVTLLVILDHFVEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFY 60

Query: 61  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMA 120
           IHVKPG Y EHVE+  QKTFIALIGDDA+TT+IVD+RS+ TGF T+ SAT  V G NFMA
Sbjct: 61  IHVKPGTYNEHVEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMA 120

Query: 121 QSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVD 180
           QS+ F+NSAGP+ GQAVA+  D A  TVYYKCVFLG+QDTL+      FFK+ +IYG+VD
Sbjct: 121 QSLTFQNSAGPDKGQAVAVL-DQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVD 180

Query: 181 FIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLN 240
           FIFG G A++QDC IY RL +   T+TAQSK  +D  SGF+FQ C+VT SPE+  +KN  
Sbjct: 181 FIFGNGLAIFQDCNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIVSSKN-K 240

Query: 241 FEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRR 300
             V+LGRPWR YS V F++S+LD VV P GW  W    P+NLLFYAEY N G G+  + R
Sbjct: 241 VTVFLGRPWRTYSMVAFIESFLDDVVQPKGWLEWPG-TPVNLLFYAEYKNSGTGSETSNR 300

Query: 301 VKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 340
           V W GFH+ +  A+   +TV  FIDG +WLPETG+PFR+ L
Sbjct: 301 VDWPGFHLFSYAAEVANFTVENFIDGTQWLPETGIPFRTGL 338

[Close]

BLAST of HG10020950 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 281.6 bits (719), Expect = 8.5e-76
Identity = 139/310 (44.84%), Postives = 197/310 (63.55%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           + +V+ DGT NF +I  A+ AA   +  R+ IHVK G+Y E+VEI+K+K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 88  TTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVY 147
            TVI  +RS   G+ T +SATF V G  F+A+ I F+N+AGPE  QAVA+ +D  +  V+
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSD-TDLGVF 333

Query: 148 YKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG--NRTTTIT 207
           Y+C   G+QDTLY +    FF++  I GTVDFIFG   A++Q C+I  + G  N+  +IT
Sbjct: 334 YRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSIT 393

Query: 208 AQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVN 267
           AQ ++  ++ +GF  Q  ++ A  +L    N     YLGRPW+ YS  +FM +Y+   +N
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT-ATYLGRPWKLYSRTVFMQNYMSDAIN 453

Query: 268 PAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGN 327
           P GW  W+    L+ L+Y EY N GPGASL RRVKW G+HV+N+ A+A  +TV+  I GN
Sbjct: 454 PVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGN 513

Query: 328 RWLPETGVPF 336
            WLP TG+ F
Sbjct: 514 LWLPSTGITF 521

[Close]

BLAST of HG10020950 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 274.2 bits (700), Expect = 1.4e-73
Identity = 137/314 (43.63%), Postives = 199/314 (63.38%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           NT+V++DG   +++I  AI  A +++  R+ I+VK G+YKE+++++K+KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 88  TTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVY 147
            T+I   R+   G  T ++AT  V G  F+A+ I F N+AGP+N QAVAL  D ++ + +
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVD-SDQSAF 359

Query: 148 YKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVR--LGNRTTTIT 207
           Y+C   G+QDTLY +    F++D EIYGT+DFIFG G A+ Q+CKIY R  L  +  TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 208 AQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVN 267
           AQ ++  +Q +GFV QN  V A+             YLGRPW+ YS  ++M++Y+  +V 
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNTYMSQLVQ 479

Query: 268 PAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGN 327
           P GW  W     L+ L+Y EYNN GPG   + RVKW G+H+++    A+ +TV  FIDG 
Sbjct: 480 PRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK-RTALSFTVGSFIDGR 539

Query: 328 RWLPETGVPFRSTL 340
           RWLP TGV F + L
Sbjct: 540 RWLPATGVTFTAGL 541

[Close]

BLAST of HG10020950 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 273.9 bits (699), Expect = 1.8e-73
Identity = 140/316 (44.30%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 26  SFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDD 85
           +++  V+LDGT NF  I  AI  A   +++RF I++K G+Y E+VEI+K+K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 86  AATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCT 145
              TVI  +RS   G+ T +SATF V G  F+A+ I F+N+AGPE  QAVAL +D ++ +
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSD-SDLS 329

Query: 146 VYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG--NRTTT 205
           V+++C   G+QDTLY +    F+++  I GTVDFIFG G  ++Q+C+I  + G  N+  T
Sbjct: 330 VFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNT 389

Query: 206 ITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYV 265
           ITAQ ++ ++Q SGF  Q  +++A  +L    N     YLGRPW+ YS  +F+ + +  V
Sbjct: 390 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTT-RTYLGRPWKLYSRTVFIRNNMSDV 449

Query: 266 VNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFID 325
           V P GW  W+A+  L+ LFY E+ N GPG+ L+ RVKW G+HV N+  +A  +TV+ FI 
Sbjct: 450 VRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIK 509

Query: 326 GNRWLPETGVPFRSTL 340
           GN WLP TGV F   L
Sbjct: 510 GNLWLPSTGVTFSDGL 523

[Close]

BLAST of HG10020950 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-73
Identity = 139/320 (43.44%), Postives = 199/320 (62.19%), Query Frame = 0


Query: 25  ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSR---FYIHVKPGIYKEHVEIEKQKTFIAL 84
           IS    VS DGT NF +I AA+AAA +N +     F I+V  GIY+E++ I K K ++ +
Sbjct: 261 ISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMM 320

Query: 85  IGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDH 144
           IGD    TV+  +RS   G+ T  SATF V   NF+A +I F N+AGPE  QAVAL    
Sbjct: 321 IGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVAL-RSG 380

Query: 145 ANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVR--LGN 204
           A+ +++Y C F  +QDTLY +    F+++ ++YGTVDFIFG    ++Q+C +Y R  + N
Sbjct: 381 ADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPN 440

Query: 205 RTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSY 264
           +   ITAQ +   +Q +G   QNC++  + +L  + N   + YLGRPW++YS  ++M SY
Sbjct: 441 QFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTYLGRPWKEYSRTVYMQSY 500

Query: 265 LDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVN 324
           +D  V P GW  W+ +  L+ L+YAEYNN GPG++ T RV W G+HVINS   A      
Sbjct: 501 IDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTG 560

Query: 325 LFIDGNRWLPETGVPFRSTL 340
           LFI+ + W+ +TGVP+ S L
Sbjct: 561 LFIEAD-WIWKTGVPYTSGL 577

[Close]

BLAST of HG10020950 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 271.9 bits (694), Expect = 6.8e-73
Identity = 136/316 (43.04%), Postives = 195/316 (61.71%), Query Frame = 0


Query: 22  VHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIAL 81
           V   ++N  V++DGT NF +I  A+ AA + + +RF I++K G Y E+VE+ K+KT I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 82  IGDDAATTVIVDSRSHATGFRTSQSATFVVKGNNFMAQSIMFENSAGPENGQAVALFNDH 141
           IGD    TVI  +RS   G+ T Q+ T  VKG  ++A+ I F NSAGP   QAVA F   
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRSG 334

Query: 142 ANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLGN-- 201
           ++ + +Y+C F G+QDTLYV+    F+++ +IYGT+DFIFG    ++Q+  +Y R  N  
Sbjct: 335 SDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPG 394

Query: 202 RTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSY 261
                TAQS+   DQ +G    NC + A+P+L   K  NF+ YLGRPWR YS  + + S+
Sbjct: 395 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKSF 454

Query: 262 LDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVN 321
           +D +++PAGW     +  L  L+Y EY N GPGA++ +RV W GF  I +  +A  +TV 
Sbjct: 455 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 514

Query: 322 LFIDGNRWLPETGVPF 336
            FIDG+ WL  TG+PF
Sbjct: 515 PFIDGSTWLNSTGIPF 528

The following BLAST results are available for this feature:

BLAST of HG10020950 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055286.1	1.4e-107	60.75	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
KAG6584281.1	2.3e-107	60.82	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584282.1	5.7e-106	60.53	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
XP_022137308.1	6.3e-105	60.41	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
TYJ99208.1	8.2e-105	59.81	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]

BLAST of HG10020950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	1.2e-77	45.37	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9LXK7	1.2e-74	44.84	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	1.7e-73	44.41	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
Q9M9W7	1.9e-72	43.63	Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9SMY7	2.5e-72	44.30	Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of HG10020950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULX7	6.5e-108	60.75	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290...	[more]
A0A6J1C9Z6	3.0e-105	60.41	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1	[more]
A0A5D3BM28	4.0e-105	59.81	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418...	[more]
A0A6J1D381	2.0e-104	57.96	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1D863	1.4e-102	56.89	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1	[more]

BLAST of HG10020950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G43270.1	8.5e-76	44.84	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05620.1	1.4e-73	43.63	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	1.8e-73	44.30	pectin methylesterase 44	[more]
AT1G02810.1	5.2e-73	43.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	6.8e-73	43.04	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 23..339 e-value: 5.0E-110 score: 369.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 29..325 e-value: 4.0E-103 score: 344.2
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 23..337
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 23..337
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..18 score: 5.0
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 53..72
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 173..182
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 26..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10020950.1	HG10020950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity