HG10020805 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020805
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionprotein NRT1/ PTR FAMILY 2.8
LocationChr05: 2586581 .. 2588483 (+)
RNA-Seq ExpressionHG10020805
SyntenyHG10020805
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGGAGTCTTCATTTCCCTCCTCCCACCCCCCTCTGCCCCCTTGCCGGAAGCTAGGAGGATGGCGCGCTGTCAAATACATCATTGGTAACAGACTTTACTTAAACATCATACATTACAAACTGATCTGTGTTTTCCTTTGGTTTTGAATGGATCTTCTTTTGAAGGGAACGAGTCTTTTGAGAAGTTGTCATCCATGAGTTTGATATCTAATATCACTGTGTATCTCAGCACCAATTACAATGTCGATGGCATTTTTGTGGTCAATGTGGTTAATATTTGGAGTGGAACCTCTAATGTTGCCACTTTAGCCGGTGCTTTCATTGCTGATACTTGTCTTGGCCGCTACCGCACCCTCCTCTATGGCTCCATTGCATCATTCTTGGTATTGCTTTACTTTTCCCTTGTTGAATTCATCAACATTTATGTCAAAATTAATCTTTTTTGTCTTTAAAAAAGGGAATGGGGACTGTTACTCTCACCGCAGCTATCCATCAGCTCAGGCCTTCACATTGCAATGCTGATGATTTAGGTCAGTGCCCACAGCCACATCTTTGGCAGCTCCTTGTTCTATTCGCCGGTCTCGGTCTGTTGTCGATTGGTGCCGGTGGCATTCGTCCCTGCAACATTGCGTTTGGAGCCGATCAATTTGACACCACCACAGAAAAGGGAAAGTCTCAATTAGAAAGCTTCTTTAATTGGTGGTACCTTTCTTTCACCATTGCTCTGCTTATAGCTCTCACTGGTGTCGTTTATGTTCAGACCAATGTAAGTTGGACCCTTGGATTTGCCATCCCCACCATTTGTTTCTTCTTCTCCATCTCGATTTTCTTGCTGGGTCGCCATACTTACATCATTGCTGAGCCCAGAGGAAGCATGTTTACTGATATGACTAGGGTCATTATTGCTGCCTGTAGAAAACGAAGACATTCTGTTTCATCTTACTCGTTTTATGACCCACCAATGGAGGATTCTTCATGTGGGGAAAAGCTTCTTCACACAGAGAGGTTCAAATGGCTGGATAGAGCTGCAATTATAGTGAACCCAGAAGAGGAATTGGACGAACAGGGAAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAAGTGGAAGGATTCAAATGCCTAGTGTCTATTATTCCCGTTTGGATATCAGGAATTGGGTGTTTCATAATATTCAATCAACCAAACACATTTGGGATTCTTCAAGCAATGCAATCAAACAGATCGATCGGACCCCATTTCAAATTCCCACCCGGCTGGATGAACTTAGCCGGTATGATATCTCTATCTATATGGATCATAGTCTACGAGAGGGTTTCCATCAAACTGGGAAAGAAAATCACCGGAAAAGAAAGAAGACTTACAATGGAACAGAGAATCACTATAGGGATCGTCTTGTCGATTCTGAGTATGGTCGTCTCAGGGATTGTCGAGAAACATCGAAGAGACGCTGCTTTGAAAAACGGATTGTTCATTTCACCGACAAGTTTCGCGTTCCTCTTACCGCAGCATGCTCTCATTGGTTTGATGGAGGCATTTGCATTGGTAGCGGTTATGGAGTTCTTCACAATGCATATGCCGGAGCATATGAGAACGGTTGCAGGAGCCATCTTCTTCCTCACACTCTCTGTAGCGAGCTATTTAAGCTCTTTGATAGTTAATCTGATACACGCTGTGACCGCAAAAACTGCAAAATCGCCATGGGTAGGCGGGCATGACCTAAACCAGAATAGGCTCGACTACTACTATTTCACGATCGCCATTATCGGAACTTTGAATCTACTGTACTTCGTGTTCTTCGCAAGTCGTTTTGTGAGGGGTTATGATAATAAAGTGAAGTTGATGGAAAATGTTCACCGGACTGATTTGCCGGTGAAGGATGAAGAATGTTGA

mRNA sequence

ATGGATTTGGAGTCTTCATTTCCCTCCTCCCACCCCCCTCTGCCCCCTTGCCGGAAGCTAGGAGGATGGCGCGCTGTCAAATACATCATTGGGAACGAGTCTTTTGAGAAGTTGTCATCCATGAGTTTGATATCTAATATCACTGTGTATCTCAGCACCAATTACAATGTCGATGGCATTTTTGTGGTCAATGTGGTTAATATTTGGAGTGGAACCTCTAATGTTGCCACTTTAGCCGGTGCTTTCATTGCTGATACTTGTCTTGGCCGCTACCGCACCCTCCTCTATGGCTCCATTGCATCATTCTTGGGAATGGGGACTGTTACTCTCACCGCAGCTATCCATCAGCTCAGGCCTTCACATTGCAATGCTGATGATTTAGGTCAGTGCCCACAGCCACATCTTTGGCAGCTCCTTGTTCTATTCGCCGGTCTCGGTCTGTTGTCGATTGGTGCCGGTGGCATTCGTCCCTGCAACATTGCGTTTGGAGCCGATCAATTTGACACCACCACAGAAAAGGGAAAGTCTCAATTAGAAAGCTTCTTTAATTGGTGGTACCTTTCTTTCACCATTGCTCTGCTTATAGCTCTCACTGGTGTCGTTTATGTTCAGACCAATGTAAGTTGGACCCTTGGATTTGCCATCCCCACCATTTGTTTCTTCTTCTCCATCTCGATTTTCTTGCTGGGTCGCCATACTTACATCATTGCTGAGCCCAGAGGAAGCATGTTTACTGATATGACTAGGGTCATTATTGCTGCCTGTAGAAAACGAAGACATTCTGTTTCATCTTACTCGTTTTATGACCCACCAATGGAGGATTCTTCATGTGGGGAAAAGCTTCTTCACACAGAGAGGTTCAAATGGCTGGATAGAGCTGCAATTATAGTGAACCCAGAAGAGGAATTGGACGAACAGGGAAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAAGTGGAAGGATTCAAATGCCTAGTGTCTATTATTCCCGTTTGGATATCAGGAATTGGGTGTTTCATAATATTCAATCAACCAAACACATTTGGGATTCTTCAAGCAATGCAATCAAACAGATCGATCGGACCCCATTTCAAATTCCCACCCGGCTGGATGAACTTAGCCGGTATGATATCTCTATCTATATGGATCATAGTCTACGAGAGGGTTTCCATCAAACTGGGAAAGAAAATCACCGGAAAAGAAAGAAGACTTACAATGGAACAGAGAATCACTATAGGGATCGTCTTGTCGATTCTGAGTATGGTCGTCTCAGGGATTGTCGAGAAACATCGAAGAGACGCTGCTTTGAAAAACGGATTGTTCATTTCACCGACAAGTTTCGCGTTCCTCTTACCGCAGCATGCTCTCATTGGTTTGATGGAGGCATTTGCATTGGTAGCGGTTATGGAGTTCTTCACAATGCATATGCCGGAGCATATGAGAACGGTTGCAGGAGCCATCTTCTTCCTCACACTCTCTGTAGCGAGCTATTTAAGCTCTTTGATAGTTAATCTGATACACGCTGTGACCGCAAAAACTGCAAAATCGCCATGGGTAGGCGGGCATGACCTAAACCAGAATAGGCTCGACTACTACTATTTCACGATCGCCATTATCGGAACTTTGAATCTACTGTACTTCGTGTTCTTCGCAAGTCGTTTTGTGAGGGGTTATGATAATAAAGTGAAGTTGATGGAAAATGTTCACCGGACTGATTTGCCGGTGAAGGATGAAGAATGTTGA

Coding sequence (CDS)

ATGGATTTGGAGTCTTCATTTCCCTCCTCCCACCCCCCTCTGCCCCCTTGCCGGAAGCTAGGAGGATGGCGCGCTGTCAAATACATCATTGGGAACGAGTCTTTTGAGAAGTTGTCATCCATGAGTTTGATATCTAATATCACTGTGTATCTCAGCACCAATTACAATGTCGATGGCATTTTTGTGGTCAATGTGGTTAATATTTGGAGTGGAACCTCTAATGTTGCCACTTTAGCCGGTGCTTTCATTGCTGATACTTGTCTTGGCCGCTACCGCACCCTCCTCTATGGCTCCATTGCATCATTCTTGGGAATGGGGACTGTTACTCTCACCGCAGCTATCCATCAGCTCAGGCCTTCACATTGCAATGCTGATGATTTAGGTCAGTGCCCACAGCCACATCTTTGGCAGCTCCTTGTTCTATTCGCCGGTCTCGGTCTGTTGTCGATTGGTGCCGGTGGCATTCGTCCCTGCAACATTGCGTTTGGAGCCGATCAATTTGACACCACCACAGAAAAGGGAAAGTCTCAATTAGAAAGCTTCTTTAATTGGTGGTACCTTTCTTTCACCATTGCTCTGCTTATAGCTCTCACTGGTGTCGTTTATGTTCAGACCAATGTAAGTTGGACCCTTGGATTTGCCATCCCCACCATTTGTTTCTTCTTCTCCATCTCGATTTTCTTGCTGGGTCGCCATACTTACATCATTGCTGAGCCCAGAGGAAGCATGTTTACTGATATGACTAGGGTCATTATTGCTGCCTGTAGAAAACGAAGACATTCTGTTTCATCTTACTCGTTTTATGACCCACCAATGGAGGATTCTTCATGTGGGGAAAAGCTTCTTCACACAGAGAGGTTCAAATGGCTGGATAGAGCTGCAATTATAGTGAACCCAGAAGAGGAATTGGACGAACAGGGAAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAAGTGGAAGGATTCAAATGCCTAGTGTCTATTATTCCCGTTTGGATATCAGGAATTGGGTGTTTCATAATATTCAATCAACCAAACACATTTGGGATTCTTCAAGCAATGCAATCAAACAGATCGATCGGACCCCATTTCAAATTCCCACCCGGCTGGATGAACTTAGCCGGTATGATATCTCTATCTATATGGATCATAGTCTACGAGAGGGTTTCCATCAAACTGGGAAAGAAAATCACCGGAAAAGAAAGAAGACTTACAATGGAACAGAGAATCACTATAGGGATCGTCTTGTCGATTCTGAGTATGGTCGTCTCAGGGATTGTCGAGAAACATCGAAGAGACGCTGCTTTGAAAAACGGATTGTTCATTTCACCGACAAGTTTCGCGTTCCTCTTACCGCAGCATGCTCTCATTGGTTTGATGGAGGCATTTGCATTGGTAGCGGTTATGGAGTTCTTCACAATGCATATGCCGGAGCATATGAGAACGGTTGCAGGAGCCATCTTCTTCCTCACACTCTCTGTAGCGAGCTATTTAAGCTCTTTGATAGTTAATCTGATACACGCTGTGACCGCAAAAACTGCAAAATCGCCATGGGTAGGCGGGCATGACCTAAACCAGAATAGGCTCGACTACTACTATTTCACGATCGCCATTATCGGAACTTTGAATCTACTGTACTTCGTGTTCTTCGCAAGTCGTTTTGTGAGGGGTTATGATAATAAAGTGAAGTTGATGGAAAATGTTCACCGGACTGATTTGCCGGTGAAGGATGAAGAATGTTGA

Protein sequence

MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC
Homology
BLAST of HG10020805 vs. NCBI nr
Match: XP_038894640.1 (protein NRT1/ PTR FAMILY 2.8 [Benincasa hispida])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 535/582 (91.92%), Postives = 559/582 (96.05%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDLESS  SSHP   P R+LGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+GI
Sbjct: 1   MDLESSLSSSHPTPTPRRRLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTQYNVNGI 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIAS LGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASLLGMGTVMLTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCNA+D G CPQPHLWQLLVLFAGLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNAEDSGHCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFTIALL+ALTGVVYVQTNVSWTLGFAIPTICFFFSISIFL+GRHTYIIAEPR
Sbjct: 181 FFNWWYLSFTIALLVALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLMGRHTYIIAEPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSMFTDM+RVIIAACR+R+HSVSSYSFYDPPMEDSSCGEKL+HTERFKWLDRAAIIVNPE
Sbjct: 241 GSMFTDMSRVIIAACRRRKHSVSSYSFYDPPMEDSSCGEKLIHTERFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKNPWRLCSLQQVEG KCLVSIIPVWISGIGCFI+FNQPNTFGILQAMQSNR
Sbjct: 301 EELDEQGKPKNPWRLCSLQQVEGLKCLVSIIPVWISGIGCFIVFNQPNTFGILQAMQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIGPHFKFPPGWMNLAGMISLSIWII+YERV IKLGKK TGKERRLTMEQRITIGIVLSI
Sbjct: 361 SIGPHFKFPPGWMNLAGMISLSIWIIIYERVFIKLGKKFTGKERRLTMEQRITIGIVLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
           LSM+VSGIVEKHRRDAALK G F+SPTSFAFL+PQHAL GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 LSMIVSGIVEKHRRDAALKTGSFVSPTSFAFLIPQHALTGLMEAFALVALMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLTLSVASYLSS +V++IH V+AKTAKSPWVGGHDLNQNRLDYYY TIA+
Sbjct: 481 HMRTVAGAIFFLTLSVASYLSSFLVDVIHIVSAKTAKSPWVGGHDLNQNRLDYYYVTIAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           IGTLNLLYFVFFASRFVRGYD+KVKL ENV R+DLPVKDEEC
Sbjct: 541 IGTLNLLYFVFFASRFVRGYDSKVKLTENVDRSDLPVKDEEC 582

BLAST of HG10020805 vs. NCBI nr
Match: XP_004152540.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KGN64260.1 hypothetical protein Csa_013601 [Cucumis sativus])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.3e-303
Identity = 514/582 (88.32%), Postives = 543/582 (93.30%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDL +  PSSHPP PPC+KLGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+G 
Sbjct: 1   MDLVAPLPSSHPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTQYNVNGT 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIW GTSN+ATLAGAFIADT LGRYRTLLYGSIASFLGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWIGTSNIATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCNADD G CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNADDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFF SISIFLLGRHTYII +PR
Sbjct: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSM TD+ RVI+AA RKR HS+SS SFYD PMEDS+CGEKL+HT+RFKWLDRAAIIVNPE
Sbjct: 241 GSMLTDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKN WRLCSLQQVEGFKCLVSIIPVWISGIGCFI+FNQPNTFGILQA+QSNR
Sbjct: 301 EELDEQGKPKNSWRLCSLQQVEGFKCLVSIIPVWISGIGCFIVFNQPNTFGILQAIQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIGPHFKFPPGWM+LAGMI+LSIWII+YERV IKLGKKITGKERRLTMEQRITIGI+LSI
Sbjct: 361 SIGPHFKFPPGWMSLAGMIALSIWIIIYERVLIKLGKKITGKERRLTMEQRITIGILLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
            SM+ SG+VEKHRRDAALKN LFISPTSFA LLPQH L GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 FSMITSGVVEKHRRDAALKNRLFISPTSFALLLPQHVLTGLMEAFALVAIMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLT+SVASYLSSLIV +I  V+AK AKSPWVGGHDLNQNRLDYYYFT+A+
Sbjct: 481 HMRTVAGAIFFLTISVASYLSSLIVYVIKKVSAKIAKSPWVGGHDLNQNRLDYYYFTLAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           + TLNLLYFV FA RFVRGYD+KVKL ENV R DLPVKDEEC
Sbjct: 541 LETLNLLYFVIFARRFVRGYDDKVKLTENVRRNDLPVKDEEC 582

BLAST of HG10020805 vs. NCBI nr
Match: XP_008437665.1 (PREDICTED: protein NRT1/ PTR FAMILY 2.8 [Cucumis melo] >TYJ99109.1 protein NRT1/ PTR FAMILY 2.8 [Cucumis melo var. makuwa])

HSP 1 Score: 1039.6 bits (2687), Expect = 9.9e-300
Identity = 511/582 (87.80%), Postives = 539/582 (92.61%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDL +  PSS PP PPC+KLGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+GI
Sbjct: 1   MDLVTPLPSSQPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNVNGI 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIW GTSNVATLAGAFIADT LGRYRTLLYGSIASFLGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWIGTSNVATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCN +D G CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNVEDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFT+ALLIALTGVVYVQTNVSWTLGFAIPTICFF SISIFLLGRHTYII +PR
Sbjct: 181 FFNWWYLSFTVALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSM  D+ RVI+AA RKR HS+SS SFYD PMEDS+CGEKL+HT+RFKWLDRAAIIVNPE
Sbjct: 241 GSMLKDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKN WRLCSLQQVEG KCLVSI+PVWISGIGCFI+FNQPNTFGILQAMQSNR
Sbjct: 301 EELDEQGKPKNSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQAMQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIG HFKFPPGWMNLAGMI+LSIWII+YERV IKLGKK+TGKERRLTMEQRITIGIVLSI
Sbjct: 361 SIGSHFKFPPGWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIGIVLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
           LSM+ SG+VEKHRRDAALKN LFISPTSFA LLPQH L GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 LSMITSGVVEKHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLT+SVASYLSSLIV++I  V+ K AKSPWVGGHDLN NRLDYYYFTIA+
Sbjct: 481 HMRTVAGAIFFLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYYFTIAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           I TLNLLYFVFFA RFVRGYD+KVKL EN  R DLPVKDEEC
Sbjct: 541 IETLNLLYFVFFARRFVRGYDDKVKLTENGRRNDLPVKDEEC 582

BLAST of HG10020805 vs. NCBI nr
Match: XP_023001041.1 (protein NRT1/ PTR FAMILY 2.8 [Cucurbita maxima])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.2e-292
Identity = 501/580 (86.38%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDLESSFPSS-----HPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNY 60
           MDLES  PSS     H P PP R+ GGW AVKYIIGNESFEKLSSMSLISNITVYL+T Y
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWCAVKYIIGNESFEKLSSMSLISNITVYLTTKY 60

Query: 61  NVDGIFVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIH 120
           N++GI+VVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMG VTLTA   
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSHCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGK 180
           QLRPS CNA +   CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYI 240
           SQLESFFNWWYLSFT+ALLIALTGVVYVQTN+SWTLGFAIPTICFFFSI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 IAEPRGSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAI 300
           +AEPRGSMF+DM RVIIAACRKRR+SVSSYSFY+PPM DSS  EKL+HTERFKWLD+AAI
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPEEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQA 360
           IVNP+EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIP+WISGIGCF++FNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 MQSNRSIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIG 420
           +QSNRSIG HFKFPPGWMNLAGMISLSIWII+YERV IK+ KKITGKERRLTM+QRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVLSILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFT 480
           I+LSI+ MVVSGIVE++RR+AALKNG FISP SFAFLLPQHAL GLMEAFALVA+MEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIH+V+ + A+SPWVGGHDLN+NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYY 540

Query: 541 FTIAIIGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDL 576
           FTIAI+GTLNLLYFV FASRFV  YDNKVKLME+++R DL
Sbjct: 541 FTIAIVGTLNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of HG10020805 vs. NCBI nr
Match: XP_023520205.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1006.9 bits (2602), Expect = 7.1e-290
Identity = 497/580 (85.69%), Postives = 535/580 (92.24%), Query Frame = 0

Query: 1   MDLESSFPSS-----HPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNY 60
           MDLES  PSS     H P PP R+ GGWRAVKYIIGNESFEKLSSMSLISNITVYLST Y
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NVDGIFVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIH 120
           N++GI+VVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMG VTLTA   
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSHCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGK 180
           QLRPS CNA +   CPQPHLWQLLVLF GLGLLS+GAGGIRPCN+AFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSVGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYI 240
           SQLESFFNWWYLSFT+ALLIALTGVVYVQTN+SWTLGFAIPTICFFFSI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 IAEPRGSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAI 300
           +AEPRGSMF+DM RVIIAACRK R+SVSSYSFYDPPM DSS  EKL+HTERFKWLD+AA 
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKGRYSVSSYSFYDPPMADSSHEEKLVHTERFKWLDKAAF 300

Query: 301 IVNPEEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQA 360
           IVNP+EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIP+WISGIGCF++FNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 MQSNRSIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIG 420
           +QSNRSIG HFKFPPGWM+LAGMISLSIWII+YERV IK+ KKITGKERRLTM+QRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVLSILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFT 480
           I+LSI+ MVVSGIVE++RR+AALKNG FISP SFAFLLPQHAL GLMEAFALVA+MEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLI  V+ + A+S WVGGHDLN+NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540

Query: 541 FTIAIIGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDL 576
           FTIAI+G LNLLYFV FASRFV  YDNKVKLME+++R DL
Sbjct: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of HG10020805 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 642.9 bits (1657), Expect = 3.5e-183
Identity = 324/558 (58.06%), Postives = 419/558 (75.09%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MD+ESS PSSH  +   ++ GGWRA+KYII NESFEKL+SMSLI N++VYL T YN+ G+
Sbjct: 1   MDVESSSPSSHALIK--KEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGV 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           F+VNV+NIW G+ N+ TLAGAF++D  LGR+ TLL GSIASF+GMG   LTAA+  LRP 
Sbjct: 61  FLVNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPD 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
            C  D      QP  WQL VLF+GLGLL+IGAGG+RPCNIAFGADQFDT+T+KGK+ LE+
Sbjct: 121 AC-IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLET 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWY SFT+AL+IALTGVVY+QTN+SW +GF IPT C   SI+ F++G+HTYI A+  
Sbjct: 181 FFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAE 240

Query: 241 GSMFTDMTRVIIAACRKRR-HSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNP 300
           GS+F D+ +V+ AAC+KR+    S  +FY  P  D S    +    R ++ D+A+I+ NP
Sbjct: 241 GSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNP 300

Query: 301 EEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSN 360
             EL+E G  K  WRLCS+QQV+  KC+ +I+PVW++GI CFI+ +Q N +GILQAMQ +
Sbjct: 301 -NELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMD 360

Query: 361 RSIGPH-FKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVL 420
           ++ GPH F+ P GWMNL  MI+L+IWI +YE V I + K+ITG+++RLT++ RI   IV+
Sbjct: 361 KTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVM 420

Query: 421 SILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHM 480
            I+ M+V+G  EK RR +ALKNG F+SP S   LLPQ AL GL EAF+ VA+MEF T+ M
Sbjct: 421 GIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRM 480

Query: 481 PEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTI 540
           PEHMR VAGAIFFL+ S+ASY+ +L++N+I AVT K  KS W+G  DLN+NRL+ Y+F I
Sbjct: 481 PEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKS-WLGDKDLNKNRLENYFFII 540

Query: 541 AIIGTLNLLYFVFFASRF 557
           A I   NLLYF  FASR+
Sbjct: 541 AGIQVANLLYFRLFASRY 551

BLAST of HG10020805 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.1e-127
Identity = 230/556 (41.37%), Postives = 367/556 (66.01%), Query Frame = 0

Query: 18  RKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVAT 77
           +K GGWRAV +I+GNE+ E+L S+ L++N  VYL+  ++++ +   NV+NIWSG +N+  
Sbjct: 50  KKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTP 109

Query: 78  LAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQ 137
           L GA+I+DT +GR++T+ + S A+ LG+ T+TLTA+  QL P+ CN+ D   C  P+  Q
Sbjct: 110 LVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQ 169

Query: 138 LLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIAL 197
           + VL  GL  LS+G+GGIRPC+I FG DQFD  TE+G   + SFFNW+Y++FT+ L+I  
Sbjct: 170 IGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQ 229

Query: 198 TGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRK 257
           T VVY+Q  VSW +GF+IPT     ++ +F  G   Y+  +P GS+F+ + +VI+AA +K
Sbjct: 230 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 289

Query: 258 RRHSV-----SSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNP 317
           R+  +      + ++YDP ++ SS   KL  + +F+ LD+AA+++  E +L  +G P + 
Sbjct: 290 RKLKLPAEDDGTVTYYDPAIK-SSVLSKLHRSNQFRCLDKAAVVI--EGDLTPEGPPADK 349

Query: 318 WRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGPHFKFPPGW 377
           WRLCS+Q+VE  KCL+ I+P+W +GI          TF + QA++ +R++GP F+ P G 
Sbjct: 350 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 409

Query: 378 MNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKH 437
           +++  ++++ I++  Y+RV +   ++ITG +  +T+ QRI  GIV +I SM+V+GIVE+ 
Sbjct: 410 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 469

Query: 438 RRDAALKNG--LFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIF 497
           RR  ++  G    ++P S  +L PQ  L+GL EAF ++  +EFF    PEHMR++A ++F
Sbjct: 470 RRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLF 529

Query: 498 FLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 557
            L+ + +SYLSS +V ++H  +    +  W+   +LN  +LDY+Y+ IA++G +NL+YF 
Sbjct: 530 SLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVYFW 589

Query: 558 FFASRFVRGYDNKVKL 567
           + A    RGY  KV L
Sbjct: 590 YCA----RGYRYKVGL 597

BLAST of HG10020805 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 434.1 bits (1115), Expect = 2.5e-120
Identity = 225/546 (41.21%), Postives = 350/546 (64.10%), Query Frame = 0

Query: 16  PCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNV 75
           P +KLGGWRA+ +I+GNE+ EKL S+ + +N  +YL   ++++ +   NV  +W G +N 
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 76  ATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHL 135
           A L GA I+D  +GR++T+ Y S+ S LG+ TVTLTA + QL P  CN     +C  P+ 
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 136 WQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLI 195
            QL +LF GLG LSIG+GGIRPC+I FG DQFD  TE+G   + SFFNW+YL+ T+ L+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 196 ALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAAC 255
           + T VVY+QT VSW +GF+IPT     ++ +F +G   Y+  +P GS+F+ + RVI+AA 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 256 RKRRHSVS-----SYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPK 315
           +KR   +S     +  +Y+PP++      KL  T++FK+LD+AA+I+  + +L  +G P 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGVL-SKLPLTDQFKFLDKAAVIL--DGDLTSEGVPA 310

Query: 316 NPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGPHFKFPP 375
           N WRLCS+Q+VE  KCL+ ++PVW +GI   +      TF + QA + +R +GPHF+ P 
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 376 GWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVE 435
             + +   I++ IW+ +YE + +    ++  ++ R+T+ QR+ IGIV +ILSM  +G VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 436 KHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIF 495
             RR  A +    ++  S  +L     L+GL E+F  + ++EFF    PEHMR++A ++F
Sbjct: 431 GVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 490

Query: 496 FLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 555
            L+ + A+YLSSL+V  +H V+       W+   DL++ +LDY+Y+ IA++G +NL+YF 
Sbjct: 491 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFW 545

Query: 556 FFASRF 557
           + A R+
Sbjct: 551 YCAHRY 545

BLAST of HG10020805 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-116
Identity = 225/539 (41.74%), Postives = 339/539 (62.89%), Query Frame = 0

Query: 22  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVATLAGA 81
           GW+ + +IIGNE+FEKL  +   SN+ +YL+T +N+  I    VVNI+ GTSN  T+  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 82  FIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQLLVL 141
           F+ D+  GRY+TL +  IA FLG   + LTA IH L P+ C  +    C  P + Q++ L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 142 FAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIALTGVV 201
              + LL IGAGGIRPCN+ FGADQFD  T++GK  +ESFFNW++ +FT A +++LT +V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 202 YVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRKRR-H 261
           YVQ+NVSW++G AIP I       IF  G   Y+  +  GS    +TRVI+ A +KRR  
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 262 SVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNPWRLCSLQQ 321
            V     Y+    D     KL HTE+F++LD++A I   +++L++ G P + W+LCS+QQ
Sbjct: 262 PVGPNELYNYIASDFK-NSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 322 VEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGP-HFKFPPGWMNLAGMI 381
           VE  KC++ ++PVW+S    ++ + Q  T+ I Q++QS+R +GP  F+ P G   +  M+
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 382 SLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKHRRDAALK 441
            ++I+I +Y+RV +   +K TG++  +T  QR+  G+ L I SM+VS IVE++RR  AL 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 442 N---GL-----FISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIFF 501
               GL      IS  S  +L+PQ  L+G+ +A A V  MEF+    PE+MR+ AG++++
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 502 LTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 551
             + +ASYLS+ +++ +H  T   +   W+   DLN+ RL+Y+YF +A + TLNL YF+
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWL-PEDLNKGRLEYFYFLVAGMMTLNLAYFL 557

BLAST of HG10020805 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.5e-114
Identity = 224/563 (39.79%), Postives = 339/563 (60.21%), Query Frame = 0

Query: 22  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVATLAGA 81
           GW+ + +IIGNE+FEKL  +  +SN+ VYL+  +N+  I    ++N +SGT N  T   A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 82  FIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQLLVL 141
           F+ DT  GRY+TL    IA FLG   + LTAA+ QL P+ C       C  P   Q+  L
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFL 165

Query: 142 FAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIALTGVV 201
             GLG L +GAGGIRPCN+AFGADQF+  +E GK  ++SFFNW++ +FT A +++LT VV
Sbjct: 166 LMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVV 225

Query: 202 YVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRKR--- 261
           YVQ+NVSWT+G  IP +  F +  IF  G   Y+  +  GS    + +VI  A +KR   
Sbjct: 226 YVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLK 285

Query: 262 ---RHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNPWRL 321
              +  ++ Y++Y P   +S    KL +T++F++LD+AAI+  PE++L   GKP +PW+L
Sbjct: 286 PAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKL 345

Query: 322 CSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGP-HFKFPPGWMN 381
           C++QQVE  KC+V ++P+W +    ++   Q  T+ + QA+QS+R +G   F  P     
Sbjct: 346 CTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYV 405

Query: 382 LAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKHRR 441
           +  M  ++++I+VY+RV +   ++ITG +  +T+ QRI  GI  +  S+VV+G VE+ RR
Sbjct: 406 VFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRR 465

Query: 442 DAALKNGLF--------ISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVA 501
             AL             IS  S  +L+PQ +L G+ EAFA +  MEF+    PE+MR+ A
Sbjct: 466 TFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFA 525

Query: 502 GAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNL 561
           G+IF++   V+SYL S ++  +H  T  ++   W+   DLN+ RLD +YF IA I  +N 
Sbjct: 526 GSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYFMIAGILAVNF 585

Query: 562 LYFVFFASRF-VRGYDNKVKLME 569
            YF+  +  +  +G D++V   E
Sbjct: 586 AYFLVMSRWYRYKGSDDEVTTYE 602

BLAST of HG10020805 vs. ExPASy TrEMBL
Match: A0A0A0LQR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045580 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.5e-303
Identity = 514/582 (88.32%), Postives = 543/582 (93.30%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDL +  PSSHPP PPC+KLGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+G 
Sbjct: 1   MDLVAPLPSSHPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTQYNVNGT 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIW GTSN+ATLAGAFIADT LGRYRTLLYGSIASFLGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWIGTSNIATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCNADD G CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNADDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFF SISIFLLGRHTYII +PR
Sbjct: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSM TD+ RVI+AA RKR HS+SS SFYD PMEDS+CGEKL+HT+RFKWLDRAAIIVNPE
Sbjct: 241 GSMLTDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKN WRLCSLQQVEGFKCLVSIIPVWISGIGCFI+FNQPNTFGILQA+QSNR
Sbjct: 301 EELDEQGKPKNSWRLCSLQQVEGFKCLVSIIPVWISGIGCFIVFNQPNTFGILQAIQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIGPHFKFPPGWM+LAGMI+LSIWII+YERV IKLGKKITGKERRLTMEQRITIGI+LSI
Sbjct: 361 SIGPHFKFPPGWMSLAGMIALSIWIIIYERVLIKLGKKITGKERRLTMEQRITIGILLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
            SM+ SG+VEKHRRDAALKN LFISPTSFA LLPQH L GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 FSMITSGVVEKHRRDAALKNRLFISPTSFALLLPQHVLTGLMEAFALVAIMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLT+SVASYLSSLIV +I  V+AK AKSPWVGGHDLNQNRLDYYYFT+A+
Sbjct: 481 HMRTVAGAIFFLTISVASYLSSLIVYVIKKVSAKIAKSPWVGGHDLNQNRLDYYYFTLAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           + TLNLLYFV FA RFVRGYD+KVKL ENV R DLPVKDEEC
Sbjct: 541 LETLNLLYFVIFARRFVRGYDDKVKLTENVRRNDLPVKDEEC 582

BLAST of HG10020805 vs. ExPASy TrEMBL
Match: A0A5D3BJC6 (Protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003140 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.8e-300
Identity = 511/582 (87.80%), Postives = 539/582 (92.61%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDL +  PSS PP PPC+KLGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+GI
Sbjct: 1   MDLVTPLPSSQPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNVNGI 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIW GTSNVATLAGAFIADT LGRYRTLLYGSIASFLGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWIGTSNVATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCN +D G CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNVEDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFT+ALLIALTGVVYVQTNVSWTLGFAIPTICFF SISIFLLGRHTYII +PR
Sbjct: 181 FFNWWYLSFTVALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSM  D+ RVI+AA RKR HS+SS SFYD PMEDS+CGEKL+HT+RFKWLDRAAIIVNPE
Sbjct: 241 GSMLKDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKN WRLCSLQQVEG KCLVSI+PVWISGIGCFI+FNQPNTFGILQAMQSNR
Sbjct: 301 EELDEQGKPKNSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQAMQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIG HFKFPPGWMNLAGMI+LSIWII+YERV IKLGKK+TGKERRLTMEQRITIGIVLSI
Sbjct: 361 SIGSHFKFPPGWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIGIVLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
           LSM+ SG+VEKHRRDAALKN LFISPTSFA LLPQH L GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 LSMITSGVVEKHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLT+SVASYLSSLIV++I  V+ K AKSPWVGGHDLN NRLDYYYFTIA+
Sbjct: 481 HMRTVAGAIFFLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYYFTIAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           I TLNLLYFVFFA RFVRGYD+KVKL EN  R DLPVKDEEC
Sbjct: 541 IETLNLLYFVFFARRFVRGYDDKVKLTENGRRNDLPVKDEEC 582

BLAST of HG10020805 vs. ExPASy TrEMBL
Match: A0A1S3AU85 (protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo OX=3656 GN=LOC103483003 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.8e-300
Identity = 511/582 (87.80%), Postives = 539/582 (92.61%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MDL +  PSS PP PPC+KLGGWRAVKYIIGNESFEKLSSMSLISNITVYLST YNV+GI
Sbjct: 1   MDLVTPLPSSQPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNVNGI 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           FVVNVVNIW GTSNVATLAGAFIADT LGRYRTLLYGSIASFLGMGTV LTAA+HQLRP 
Sbjct: 61  FVVNVVNIWIGTSNVATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALHQLRPP 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
           HCN +D G CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGKSQLES
Sbjct: 121 HCNVEDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLES 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWYLSFT+ALLIALTGVVYVQTNVSWTLGFAIPTICFF SISIFLLGRHTYII +PR
Sbjct: 181 FFNWWYLSFTVALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPR 240

Query: 241 GSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPE 300
           GSM  D+ RVI+AA RKR HS+SS SFYD PMEDS+CGEKL+HT+RFKWLDRAAIIVNPE
Sbjct: 241 GSMLKDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPE 300

Query: 301 EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNR 360
           EELDEQGKPKN WRLCSLQQVEG KCLVSI+PVWISGIGCFI+FNQPNTFGILQAMQSNR
Sbjct: 301 EELDEQGKPKNSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQAMQSNR 360

Query: 361 SIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSI 420
           SIG HFKFPPGWMNLAGMI+LSIWII+YERV IKLGKK+TGKERRLTMEQRITIGIVLSI
Sbjct: 361 SIGSHFKFPPGWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIGIVLSI 420

Query: 421 LSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPE 480
           LSM+ SG+VEKHRRDAALKN LFISPTSFA LLPQH L GLMEAFALVA+MEFFTMHMPE
Sbjct: 421 LSMITSGVVEKHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFTMHMPE 480

Query: 481 HMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAI 540
           HMRTVAGAIFFLT+SVASYLSSLIV++I  V+ K AKSPWVGGHDLN NRLDYYYFTIA+
Sbjct: 481 HMRTVAGAIFFLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYYFTIAV 540

Query: 541 IGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDLPVKDEEC 583
           I TLNLLYFVFFA RFVRGYD+KVKL EN  R DLPVKDEEC
Sbjct: 541 IETLNLLYFVFFARRFVRGYDDKVKLTENGRRNDLPVKDEEC 582

BLAST of HG10020805 vs. ExPASy TrEMBL
Match: A0A6J1KHH5 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita maxima OX=3661 GN=LOC111495296 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.7e-293
Identity = 501/580 (86.38%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDLESSFPSS-----HPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNY 60
           MDLES  PSS     H P PP R+ GGW AVKYIIGNESFEKLSSMSLISNITVYL+T Y
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWCAVKYIIGNESFEKLSSMSLISNITVYLTTKY 60

Query: 61  NVDGIFVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIH 120
           N++GI+VVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMG VTLTA   
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSHCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGK 180
           QLRPS CNA +   CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYI 240
           SQLESFFNWWYLSFT+ALLIALTGVVYVQTN+SWTLGFAIPTICFFFSI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 IAEPRGSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAI 300
           +AEPRGSMF+DM RVIIAACRKRR+SVSSYSFY+PPM DSS  EKL+HTERFKWLD+AAI
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPEEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQA 360
           IVNP+EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIP+WISGIGCF++FNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 MQSNRSIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIG 420
           +QSNRSIG HFKFPPGWMNLAGMISLSIWII+YERV IK+ KKITGKERRLTM+QRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVLSILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFT 480
           I+LSI+ MVVSGIVE++RR+AALKNG FISP SFAFLLPQHAL GLMEAFALVA+MEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIH+V+ + A+SPWVGGHDLN+NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYY 540

Query: 541 FTIAIIGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDL 576
           FTIAI+GTLNLLYFV FASRFV  YDNKVKLME+++R DL
Sbjct: 541 FTIAIVGTLNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of HG10020805 vs. ExPASy TrEMBL
Match: A0A6J1E886 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita moschata OX=3662 GN=LOC111431644 PE=3 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 7.2e-288
Identity = 493/580 (85.00%), Postives = 534/580 (92.07%), Query Frame = 0

Query: 1   MDLESSFPSS-----HPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNY 60
           MDLES  PSS     H   PP R+ GGWRAVKYIIGNESFEKLSSMSLISNITVYLST Y
Sbjct: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NVDGIFVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIH 120
           N++GI+VVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMG VTLTA   
Sbjct: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120

Query: 121 QLRPSHCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGK 180
           QLRPS CNA +   CPQPHLWQLLVLF GLGLLSIGAGGIRPCN+AFGADQFDTTTEKGK
Sbjct: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYI 240
           SQLESFFNWWYLSFT+ALLIALTGVVYVQTN+SWTLGFAIPT+CFFFSI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240

Query: 241 IAEPRGSMFTDMTRVIIAACRKRRHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAI 300
           +AEPRGSMF+DM RVII+ACRKRR+SVSSYSFYDP M DSS  EKL+HTERFKWLD+AAI
Sbjct: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPEEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQA 360
           IVNP+EELDEQGKPKNPWRLCSLQQVEGFKCLVSIIP+WISGIGCF++FNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 MQSNRSIGPHFKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIG 420
           +QSNRSIG HFKFPPGWM+LAGMISLSIWII+YERV IK+ KKITGKERRLTM+QRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVLSILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFT 480
           IV+SI+ MVVSGIVE++RR+AAL+NG FISP SFAFLLPQHAL GLMEAFALVA+MEFFT
Sbjct: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLI  V+ + A+S WVGGHDLN+NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540

Query: 541 FTIAIIGTLNLLYFVFFASRFVRGYDNKVKLMENVHRTDL 576
           FTIAI+G LNLLYFV FASRFV  YDNKVKLME+++R DL
Sbjct: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of HG10020805 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 642.9 bits (1657), Expect = 2.5e-184
Identity = 324/558 (58.06%), Postives = 419/558 (75.09%), Query Frame = 0

Query: 1   MDLESSFPSSHPPLPPCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGI 60
           MD+ESS PSSH  +   ++ GGWRA+KYII NESFEKL+SMSLI N++VYL T YN+ G+
Sbjct: 1   MDVESSSPSSHALIK--KEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGV 60

Query: 61  FVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPS 120
           F+VNV+NIW G+ N+ TLAGAF++D  LGR+ TLL GSIASF+GMG   LTAA+  LRP 
Sbjct: 61  FLVNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPD 120

Query: 121 HCNADDLGQCPQPHLWQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLES 180
            C  D      QP  WQL VLF+GLGLL+IGAGG+RPCNIAFGADQFDT+T+KGK+ LE+
Sbjct: 121 AC-IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLET 180

Query: 181 FFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPR 240
           FFNWWY SFT+AL+IALTGVVY+QTN+SW +GF IPT C   SI+ F++G+HTYI A+  
Sbjct: 181 FFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAE 240

Query: 241 GSMFTDMTRVIIAACRKRR-HSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNP 300
           GS+F D+ +V+ AAC+KR+    S  +FY  P  D S    +    R ++ D+A+I+ NP
Sbjct: 241 GSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNP 300

Query: 301 EEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSN 360
             EL+E G  K  WRLCS+QQV+  KC+ +I+PVW++GI CFI+ +Q N +GILQAMQ +
Sbjct: 301 -NELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMD 360

Query: 361 RSIGPH-FKFPPGWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVL 420
           ++ GPH F+ P GWMNL  MI+L+IWI +YE V I + K+ITG+++RLT++ RI   IV+
Sbjct: 361 KTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVM 420

Query: 421 SILSMVVSGIVEKHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHM 480
            I+ M+V+G  EK RR +ALKNG F+SP S   LLPQ AL GL EAF+ VA+MEF T+ M
Sbjct: 421 GIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRM 480

Query: 481 PEHMRTVAGAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTI 540
           PEHMR VAGAIFFL+ S+ASY+ +L++N+I AVT K  KS W+G  DLN+NRL+ Y+F I
Sbjct: 481 PEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKS-WLGDKDLNKNRLENYFFII 540

Query: 541 AIIGTLNLLYFVFFASRF 557
           A I   NLLYF  FASR+
Sbjct: 541 AGIQVANLLYFRLFASRY 551

BLAST of HG10020805 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.5e-128
Identity = 230/556 (41.37%), Postives = 367/556 (66.01%), Query Frame = 0

Query: 18  RKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVAT 77
           +K GGWRAV +I+GNE+ E+L S+ L++N  VYL+  ++++ +   NV+NIWSG +N+  
Sbjct: 50  KKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTP 109

Query: 78  LAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQ 137
           L GA+I+DT +GR++T+ + S A+ LG+ T+TLTA+  QL P+ CN+ D   C  P+  Q
Sbjct: 110 LVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQ 169

Query: 138 LLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIAL 197
           + VL  GL  LS+G+GGIRPC+I FG DQFD  TE+G   + SFFNW+Y++FT+ L+I  
Sbjct: 170 IGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQ 229

Query: 198 TGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRK 257
           T VVY+Q  VSW +GF+IPT     ++ +F  G   Y+  +P GS+F+ + +VI+AA +K
Sbjct: 230 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 289

Query: 258 RRHSV-----SSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNP 317
           R+  +      + ++YDP ++ SS   KL  + +F+ LD+AA+++  E +L  +G P + 
Sbjct: 290 RKLKLPAEDDGTVTYYDPAIK-SSVLSKLHRSNQFRCLDKAAVVI--EGDLTPEGPPADK 349

Query: 318 WRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGPHFKFPPGW 377
           WRLCS+Q+VE  KCL+ I+P+W +GI          TF + QA++ +R++GP F+ P G 
Sbjct: 350 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 409

Query: 378 MNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKH 437
           +++  ++++ I++  Y+RV +   ++ITG +  +T+ QRI  GIV +I SM+V+GIVE+ 
Sbjct: 410 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 469

Query: 438 RRDAALKNG--LFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIF 497
           RR  ++  G    ++P S  +L PQ  L+GL EAF ++  +EFF    PEHMR++A ++F
Sbjct: 470 RRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLF 529

Query: 498 FLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 557
            L+ + +SYLSS +V ++H  +    +  W+   +LN  +LDY+Y+ IA++G +NL+YF 
Sbjct: 530 SLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVYFW 589

Query: 558 FFASRFVRGYDNKVKL 567
           + A    RGY  KV L
Sbjct: 590 YCA----RGYRYKVGL 597

BLAST of HG10020805 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.8e-121
Identity = 225/546 (41.21%), Postives = 350/546 (64.10%), Query Frame = 0

Query: 16  PCRKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNV 75
           P +KLGGWRA+ +I+GNE+ EKL S+ + +N  +YL   ++++ +   NV  +W G +N 
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 76  ATLAGAFIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHL 135
           A L GA I+D  +GR++T+ Y S+ S LG+ TVTLTA + QL P  CN     +C  P+ 
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 136 WQLLVLFAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLI 195
            QL +LF GLG LSIG+GGIRPC+I FG DQFD  TE+G   + SFFNW+YL+ T+ L+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 196 ALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAAC 255
           + T VVY+QT VSW +GF+IPT     ++ +F +G   Y+  +P GS+F+ + RVI+AA 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 256 RKRRHSVS-----SYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPK 315
           +KR   +S     +  +Y+PP++      KL  T++FK+LD+AA+I+  + +L  +G P 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGVL-SKLPLTDQFKFLDKAAVIL--DGDLTSEGVPA 310

Query: 316 NPWRLCSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGPHFKFPP 375
           N WRLCS+Q+VE  KCL+ ++PVW +GI   +      TF + QA + +R +GPHF+ P 
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 376 GWMNLAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVE 435
             + +   I++ IW+ +YE + +    ++  ++ R+T+ QR+ IGIV +ILSM  +G VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 436 KHRRDAALKNGLFISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIF 495
             RR  A +    ++  S  +L     L+GL E+F  + ++EFF    PEHMR++A ++F
Sbjct: 431 GVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 490

Query: 496 FLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 555
            L+ + A+YLSSL+V  +H V+       W+   DL++ +LDY+Y+ IA++G +NL+YF 
Sbjct: 491 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFW 545

Query: 556 FFASRF 557
           + A R+
Sbjct: 551 YCAHRY 545

BLAST of HG10020805 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 9.1e-118
Identity = 225/539 (41.74%), Postives = 339/539 (62.89%), Query Frame = 0

Query: 22  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVATLAGA 81
           GW+ + +IIGNE+FEKL  +   SN+ +YL+T +N+  I    VVNI+ GTSN  T+  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 82  FIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQLLVL 141
           F+ D+  GRY+TL +  IA FLG   + LTA IH L P+ C  +    C  P + Q++ L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 142 FAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIALTGVV 201
              + LL IGAGGIRPCN+ FGADQFD  T++GK  +ESFFNW++ +FT A +++LT +V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 202 YVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRKRR-H 261
           YVQ+NVSW++G AIP I       IF  G   Y+  +  GS    +TRVI+ A +KRR  
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 262 SVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNPWRLCSLQQ 321
            V     Y+    D     KL HTE+F++LD++A I   +++L++ G P + W+LCS+QQ
Sbjct: 262 PVGPNELYNYIASDFK-NSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 322 VEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGP-HFKFPPGWMNLAGMI 381
           VE  KC++ ++PVW+S    ++ + Q  T+ I Q++QS+R +GP  F+ P G   +  M+
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 382 SLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKHRRDAALK 441
            ++I+I +Y+RV +   +K TG++  +T  QR+  G+ L I SM+VS IVE++RR  AL 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 442 N---GL-----FISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVAGAIFF 501
               GL      IS  S  +L+PQ  L+G+ +A A V  MEF+    PE+MR+ AG++++
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 502 LTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNLLYFV 551
             + +ASYLS+ +++ +H  T   +   W+   DLN+ RL+Y+YF +A + TLNL YF+
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWL-PEDLNKGRLEYFYFLVAGMMTLNLAYFL 557

BLAST of HG10020805 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.5e-115
Identity = 224/563 (39.79%), Postives = 339/563 (60.21%), Query Frame = 0

Query: 22  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTNYNVDGIFVVNVVNIWSGTSNVATLAGA 81
           GW+ + +IIGNE+FEKL  +  +SN+ VYL+  +N+  I    ++N +SGT N  T   A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 82  FIADTCLGRYRTLLYGSIASFLGMGTVTLTAAIHQLRPSHCNADDLGQCPQPHLWQLLVL 141
           F+ DT  GRY+TL    IA FLG   + LTAA+ QL P+ C       C  P   Q+  L
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFL 165

Query: 142 FAGLGLLSIGAGGIRPCNIAFGADQFDTTTEKGKSQLESFFNWWYLSFTIALLIALTGVV 201
             GLG L +GAGGIRPCN+AFGADQF+  +E GK  ++SFFNW++ +FT A +++LT VV
Sbjct: 166 LMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVV 225

Query: 202 YVQTNVSWTLGFAIPTICFFFSISIFLLGRHTYIIAEPRGSMFTDMTRVIIAACRKR--- 261
           YVQ+NVSWT+G  IP +  F +  IF  G   Y+  +  GS    + +VI  A +KR   
Sbjct: 226 YVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLK 285

Query: 262 ---RHSVSSYSFYDPPMEDSSCGEKLLHTERFKWLDRAAIIVNPEEELDEQGKPKNPWRL 321
              +  ++ Y++Y P   +S    KL +T++F++LD+AAI+  PE++L   GKP +PW+L
Sbjct: 286 PAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKL 345

Query: 322 CSLQQVEGFKCLVSIIPVWISGIGCFIIFNQPNTFGILQAMQSNRSIGP-HFKFPPGWMN 381
           C++QQVE  KC+V ++P+W +    ++   Q  T+ + QA+QS+R +G   F  P     
Sbjct: 346 CTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYV 405

Query: 382 LAGMISLSIWIIVYERVSIKLGKKITGKERRLTMEQRITIGIVLSILSMVVSGIVEKHRR 441
           +  M  ++++I+VY+RV +   ++ITG +  +T+ QRI  GI  +  S+VV+G VE+ RR
Sbjct: 406 VFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRR 465

Query: 442 DAALKNGLF--------ISPTSFAFLLPQHALIGLMEAFALVAVMEFFTMHMPEHMRTVA 501
             AL             IS  S  +L+PQ +L G+ EAFA +  MEF+    PE+MR+ A
Sbjct: 466 TFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFA 525

Query: 502 GAIFFLTLSVASYLSSLIVNLIHAVTAKTAKSPWVGGHDLNQNRLDYYYFTIAIIGTLNL 561
           G+IF++   V+SYL S ++  +H  T  ++   W+   DLN+ RLD +YF IA I  +N 
Sbjct: 526 GSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYFMIAGILAVNF 585

Query: 562 LYFVFFASRF-VRGYDNKVKLME 569
            YF+  +  +  +G D++V   E
Sbjct: 586 AYFLVMSRWYRYKGSDDEVTTYE 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894640.10.0e+0091.92protein NRT1/ PTR FAMILY 2.8 [Benincasa hispida][more]
XP_004152540.17.3e-30388.32protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KGN64260.1 hypothetical protein ... [more]
XP_008437665.19.9e-30087.80PREDICTED: protein NRT1/ PTR FAMILY 2.8 [Cucumis melo] >TYJ99109.1 protein NRT1/... [more]
XP_023001041.11.2e-29286.38protein NRT1/ PTR FAMILY 2.8 [Cucurbita maxima][more]
XP_023520205.17.1e-29085.69protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q3E8X33.5e-18358.06Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2[more]
Q8RX772.1e-12741.37Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX92.5e-12041.21Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Q9M9V71.3e-11641.74Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1[more]
Q9LV103.5e-11439.79Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0LQR53.5e-30388.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045580 PE=3 SV=1[more]
A0A5D3BJC64.8e-30087.80Protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AU854.8e-30087.80protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo OX=3656 GN=LOC103483003 PE=3 SV=1[more]
A0A6J1KHH55.7e-29386.38protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita maxima OX=3661 GN=LOC111495296 PE=3 SV... [more]
A0A6J1E8867.2e-28885.00protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita moschata OX=3662 GN=LOC111431644 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G28470.12.5e-18458.06Major facilitator superfamily protein [more]
AT1G69870.11.5e-12841.37nitrate transporter 1.7 [more]
AT1G27080.11.8e-12141.21nitrate transporter 1.6 [more]
AT1G18880.19.1e-11841.74Major facilitator superfamily protein [more]
AT5G62680.12.5e-11539.79Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 91..521
e-value: 4.6E-78
score: 262.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 14..561
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 14..563
e-value: 3.7E-155
score: 519.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 40..553
NoneNo IPR availablePANTHERPTHR11654:SF181PROTEIN NRT1/ PTR FAMILY 2.8coord: 14..561
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 27..556
e-value: 0.0
score: 524.908

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020805.1HG10020805.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity