HG10020785 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020785
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationChr05: 2429211 .. 2430367 (+)
RNA-Seq ExpressionHG10020785
SyntenyHG10020785
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGGTACCACAGAAATCTCTAACCTTAAAACTCCACATCGAAATTAAACGGAAATCAACTCATCAATTTCTTCATCTCTGTTCTTTTTTGTTGCAGATTGGCCGGTAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTTTTCTTGGAGAACGGAGCCAAAGTGGTGATCGCCGATGTCCAGGACGAACTCGCCGCTGCCCTCTGCAAGGAGCTTGACGACACCAGCGGCGATGTTTCCTATTTCCACTGCGATGTCACCGACGAAGCCGATATCTCGAACGCCGTTGACTTCGCTGTTGAAAAGTACGGAAAGCTAGACATCATGTTCAACAACGCTGGCATCCGTGGCAATGTAACCGCCACGACGTTGACCACAGATATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTACTTTCATGGGAGCTAAACACGCTGCCAGGTGAATTCAAAAGTTTAAATCTCCTTTGATAACAAAACAAAATCTTAAGTTTAACAAATTGGTTACAGAAATCAATAAATTTAAATTTCTATCCGTGTCCGCGATAAATATTGATAGATGTCTATAGTGACATTTTACTATGTTGGAAAACAACCCAAATTTAAATTAATGAGTCATACAAAATTTAATTTAATTTAATTGTTTATTCTTATTCTTTTCGTTAGAGTGATGGTTCCGGCAAAAACAGGGTGCATTATGTTTACGTCAAGCATGGCATCGGTGATATGCAGCGGTAACACGCCAGCGTATTCGGCGTCGAAGCATGCAATTGTAGGATTGATGAAGACGCTAGCCGTGGAATTGGGGCCTCACGGGATTAGAGTGAACGCGATATCGCCATTTGCGACAGTGACGCCGATGTTGCTGCCGTCGAAGGATGTGGAGCAGAAGAAGGCAATGGAGGGGTTCATTTCTTTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTATGTATTTAGGAAGTGATGAGTCTAAGTACGTGAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAACTTAATGCCAAAGCTTATGGACAATGA

mRNA sequence

ATGAGTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGATTGGCCGGTAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTTTTCTTGGAGAACGGAGCCAAAGTGGTGATCGCCGATGTCCAGGACGAACTCGCCGCTGCCCTCTGCAAGGAGCTTGACGACACCAGCGGCGATGTTTCCTATTTCCACTGCGATGTCACCGACGAAGCCGATATCTCGAACGCCGTTGACTTCGCTGTTGAAAAGTACGGAAAGCTAGACATCATGTTCAACAACGCTGGCATCCGTGGCAATGTAACCGCCACGACGTTGACCACAGATATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTACTTTCATGGGAGCTAAACACGCTGCCAGAGTGATGGTTCCGGCAAAAACAGGGTGCATTATGTTTACGTCAAGCATGGCATCGGTGATATGCAGCGGTAACACGCCAGCGTATTCGGCGTCGAAGCATGCAATTGTAGGATTGATGAAGACGCTAGCCGTGGAATTGGGGCCTCACGGGATTAGAGTGAACGCGATATCGCCATTTGCGACAGTGACGCCGATGTTGCTGCCGTCGAAGGATGTGGAGCAGAAGAAGGCAATGGAGGGGTTCATTTCTTTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTATGTATTTAGGAAGTGATGAGTCTAAGTACGTGAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAACTTAATGCCAAAGCTTATGGACAATGA

Coding sequence (CDS)

ATGAGTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGATTGGCCGGTAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTTTTCTTGGAGAACGGAGCCAAAGTGGTGATCGCCGATGTCCAGGACGAACTCGCCGCTGCCCTCTGCAAGGAGCTTGACGACACCAGCGGCGATGTTTCCTATTTCCACTGCGATGTCACCGACGAAGCCGATATCTCGAACGCCGTTGACTTCGCTGTTGAAAAGTACGGAAAGCTAGACATCATGTTCAACAACGCTGGCATCCGTGGCAATGTAACCGCCACGACGTTGACCACAGATATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTACTTTCATGGGAGCTAAACACGCTGCCAGAGTGATGGTTCCGGCAAAAACAGGGTGCATTATGTTTACGTCAAGCATGGCATCGGTGATATGCAGCGGTAACACGCCAGCGTATTCGGCGTCGAAGCATGCAATTGTAGGATTGATGAAGACGCTAGCCGTGGAATTGGGGCCTCACGGGATTAGAGTGAACGCGATATCGCCATTTGCGACAGTGACGCCGATGTTGCTGCCGTCGAAGGATGTGGAGCAGAAGAAGGCAATGGAGGGGTTCATTTCTTTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTATGTATTTAGGAAGTGATGAGTCTAAGTACGTGAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAACTTAATGCCAAAGCTTATGGACAATGA

Protein sequence

MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFKKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKAAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ
Homology
BLAST of HG10020785 vs. NCBI nr
Match: XP_008437356.1 (PREDICTED: zerumbone synthase [Cucumis melo] >TYJ99091.1 zerumbone synthase [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.2e-133
Identity = 250/280 (89.29%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVS+SISKRLAGKVALITGGASGIGESTVRLF+ENGAKVV+ADVQD+L A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  DDTSG-DVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD +G +VSYFHCDVTDE+DISNAVD+AVEKYGKLDIMFNNAGIRG+V ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFG+FMGAKHAARVM PAKTGCI+FTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAME FISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLNLVVDGGFSLTNPSFELNAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

BLAST of HG10020785 vs. NCBI nr
Match: XP_022994653.1 (zerumbone synthase-like [Cucurbita maxima])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 249/280 (88.93%), Postives = 266/280 (95.00%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVSA+ISKRLAGKVALITGGASGIG STVRLF+ENGAKVVIADVQD+LAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVENGAKVVIADVQDDLAAALCKEL 60

Query: 61  DD-TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD T+ DVSYFHCDVTDE+DISNAVDFAV+KYGKLDIMFNNAGIRG+VTATT+ TDMNDF
Sbjct: 61  DDATNHDVSYFHCDVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFGTFMGAKHAARVMVPAK+GCI+FTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS D EQKKAME  I LSGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDAEQKKAMETLIYLSGNLKGAMMDVEDIAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLN+V+DGGFSLTNPSFEL+ KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

BLAST of HG10020785 vs. NCBI nr
Match: XP_004152436.1 (zerumbone synthase [Cucumis sativus] >KGN64239.1 hypothetical protein Csa_014346 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.9e-132
Identity = 245/279 (87.81%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVS+SISKRLAGKVALITGGASGIGESTVRLF+ENGAKVV+ADVQD+L A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFK 120
           DDT  +VSYFHCDVTDE+DISNAVD+AVEKYGKLDIMFNNAGIRG+V +TTLT DMNDF+
Sbjct: 61  DDTGFNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGSTTLTADMNDFR 120

Query: 121 KVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
           KVFDVNVFG+FMGAKHAARVM PAKTGCI+FTSSMASVICSGN PAY+ASKHAI+GLMKT
Sbjct: 121 KVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMKT 180

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKA 240
           LAVELG  GIRVNAISP+ATVTPML+PS++VE+KKAME FISLSGNL+GAVMEAEDVAKA
Sbjct: 181 LAVELGSQGIRVNAISPYATVTPMLVPSRNVEEKKAMEAFISLSGNLQGAVMEAEDVAKA 240

Query: 241 AMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           A+YLGSDESKYVSGLNLVVDGGFSLTNPSFELNAK Y Q
Sbjct: 241 ALYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKIYAQ 279

BLAST of HG10020785 vs. NCBI nr
Match: KAG6573007.1 (Zerumbone synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 482.3 bits (1240), Expect = 2.9e-132
Identity = 247/280 (88.21%), Postives = 267/280 (95.36%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           M+SNGVSA+ISKRLAGKVALITGGASGIG STVRLF++NGAKVVIADVQD+LAAALCKEL
Sbjct: 1   MTSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  DD-TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD T  DVSYFHCDVT+E+DISNAVDFAV+KYGKLDIMFNNAGIRG+VTATT+ TDMNDF
Sbjct: 61  DDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFGTFMGAKHAARVMVPAK+GCI+FTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFATVTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLN+V+DGGFSLTNPSFEL+ KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

BLAST of HG10020785 vs. NCBI nr
Match: XP_023542445.1 (zerumbone synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 480.3 bits (1235), Expect = 1.1e-131
Identity = 246/280 (87.86%), Postives = 266/280 (95.00%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVSA+ISKRLAGKVALITGGASGIG STVRLF++NGAKVVIADVQD+LAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  DD-TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD T  DVSYFHCDVT+E+DISNAVDFAV+KYGKLDIMFNNAGIRG+VTATT+ TDMNDF
Sbjct: 61  DDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFGTFMGAKHAARVMVPAK+GCI+FTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNA+SPFA VTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAMSPFAAVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLN+V+DGGFSLTNPSFEL+ KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

BLAST of HG10020785 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.3e-70
Identity = 140/267 (52.43%), Postives = 183/267 (68.54%), Query Frame = 0

Query: 13  RLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDDTSGD--VSYF 72
           RL GKVAL+TGGASGIGES  RLF+E+GAK+ I DVQDEL   + + L    GD    YF
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRL---GGDPHACYF 61

Query: 73  HCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFKKVFDVNVFGT 132
           HCDVT E D+  AVDF  EKYG +DIM NNAGI G+        D N+FKKVFD+NV G 
Sbjct: 62  HCDVTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGV 121

Query: 133 FMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGI 192
           F+G KHAAR+M+P   G I+  +S++SVI       Y+ +KHA+VGL K++A ELG HGI
Sbjct: 122 FLGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGI 181

Query: 193 RVNAISPFATVTPMLLP--SKDVEQKKAMEGFISL---SGNLKGAVMEAEDVAKAAMYLG 252
           RVN +SP+A  T + +P   +   Q+ A+ GF++    + NLKG  +   DVA+A +YL 
Sbjct: 182 RVNCVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLA 241

Query: 253 SDESKYVSGLNLVVDGGFSLTNPSFEL 273
           ++ESKYVSGLNLV+DGGFS+ N + ++
Sbjct: 242 TEESKYVSGLNLVIDGGFSIANHTLQV 265

BLAST of HG10020785 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.4e-67
Identity = 136/266 (51.13%), Postives = 186/266 (69.92%), Query Frame = 0

Query: 2   SSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELD 61
           +++ V  +I++RL GKVALITGGASGIGE+T +LF ++GAKV IADVQDEL  ++ + + 
Sbjct: 3   ATSQVLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIG 62

Query: 62  DTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFKK 121
            ++   +Y HCDVT+E  + NAVD  V  YGKLDIMF+NAGI        +  +  DF++
Sbjct: 63  TSNS--TYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFER 122

Query: 122 VFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTL 181
           VF VNV G F+  KHAARVM+PA++G I+ T+S++S +  G++ AY  SKHA++GL + L
Sbjct: 123 VFSVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNL 182

Query: 182 AVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKAA 241
           AVELG  GIRVN +SPF   T +      ++ ++  E  I+ +GNLKG     EDVA AA
Sbjct: 183 AVELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAA 242

Query: 242 MYLGSDESKYVSGLNLVVDGGFSLTN 268
           +YL SDE+KYVSG NL +DGGFS+ N
Sbjct: 243 LYLASDEAKYVSGHNLFIDGGFSVCN 266

BLAST of HG10020785 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.3e-66
Identity = 133/271 (49.08%), Postives = 180/271 (66.42%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           M S    AS + RL  KVA+ITGGA GIGE+T +LF+  GAKVVIAD+ D+    +C  +
Sbjct: 1   MGSTSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNI 60

Query: 61  DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFK 120
             +   +S+ HCDVT + D+ N VD  + K+GKLDIMF N G+      + L     DFK
Sbjct: 61  -GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFK 120

Query: 121 KVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVIC-SGNTPAYSASKHAIVGLMK 180
           +V D+NV+G F+ AKHAARVM+PAK G I+FT+S++S     G +  Y+A+KHA++GL  
Sbjct: 121 RVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTT 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           +L  ELG HGIRVN +SP+   +P+L     V+  + +E     + NLKG ++ AEDVA 
Sbjct: 181 SLCTELGQHGIRVNCVSPYVVASPLLTDVFGVDSSR-VEELAHQAANLKGILLRAEDVAD 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSF 271
           A  YL  DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

BLAST of HG10020785 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.2e-65
Identity = 131/271 (48.34%), Postives = 179/271 (66.05%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           M S     S + RL  KVA+ITGGA GIGE+T +LF+  GAKVVIAD+ D+    +C  +
Sbjct: 1   MGSTSTPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNI 60

Query: 61  DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFK 120
             +   +S+ HCDVT + D+ N VD  + K+GKLDIMF N G+      + L     DFK
Sbjct: 61  -GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFK 120

Query: 121 KVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVIC-SGNTPAYSASKHAIVGLMK 180
           +V D+NV+G F+ AKHAARVM+PAK G I+FT+S++S     G +  Y+A+KHA++GL  
Sbjct: 121 RVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTT 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           +L  ELG +GIRVN +SP+   +P+L     V+  + +E     + NLKG ++ AEDVA 
Sbjct: 181 SLCTELGEYGIRVNCVSPYIVASPLLTDVFGVDSSR-VEELAHQAANLKGTLLRAEDVAD 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSF 271
           A  YL  DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

BLAST of HG10020785 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.3e-65
Identity = 135/269 (50.19%), Postives = 187/269 (69.52%), Query Frame = 0

Query: 3   SNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDD 62
           S+ VSA  +++L GKVA+ITGGASGIG  T RLF+++GA+VV+AD+QDEL A+L  EL  
Sbjct: 5   SSHVSAD-ARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGP 64

Query: 63  TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFKKV 122
            +   SY HCDVT+E D++ AVD AV ++GKLD+MFNNAG+ G            DF++V
Sbjct: 65  DAS--SYVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERV 124

Query: 123 FDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLA 182
             VN+ G F+G KHAARVM PA+ G I+ T+S++S +    + AY+ SKHA+VG  +  A
Sbjct: 125 LAVNLVGPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAA 184

Query: 183 VELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGA-VMEAEDVAKAA 242
            ELG HGIRVN +SP    TP+   +  ++  +A+E  ++ S NLKGA  ++A+D+A AA
Sbjct: 185 GELGRHGIRVNCVSPAGVATPLARAAMGMDD-EAIEAIMANSANLKGAGALKADDIAAAA 244

Query: 243 MYLGSDESKYVSGLNLVVDGGFSLTNPSF 271
           ++L SD+ +YVSG NL VDGG S+ N SF
Sbjct: 245 LFLASDDGRYVSGQNLRVDGGLSVVNSSF 269

BLAST of HG10020785 vs. ExPASy TrEMBL
Match: A0A5D3BK37 (Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002950 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.7e-134
Identity = 250/280 (89.29%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVS+SISKRLAGKVALITGGASGIGESTVRLF+ENGAKVV+ADVQD+L A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  DDTSG-DVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD +G +VSYFHCDVTDE+DISNAVD+AVEKYGKLDIMFNNAGIRG+V ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFG+FMGAKHAARVM PAKTGCI+FTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAME FISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLNLVVDGGFSLTNPSFELNAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

BLAST of HG10020785 vs. ExPASy TrEMBL
Match: A0A1S3AUF2 (zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.7e-134
Identity = 250/280 (89.29%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVS+SISKRLAGKVALITGGASGIGESTVRLF+ENGAKVV+ADVQD+L A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  DDTSG-DVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD +G +VSYFHCDVTDE+DISNAVD+AVEKYGKLDIMFNNAGIRG+V ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFG+FMGAKHAARVM PAKTGCI+FTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAME FISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLNLVVDGGFSLTNPSFELNAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

BLAST of HG10020785 vs. ExPASy TrEMBL
Match: A0A6J1K5R5 (zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 249/280 (88.93%), Postives = 266/280 (95.00%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVSA+ISKRLAGKVALITGGASGIG STVRLF+ENGAKVVIADVQD+LAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVENGAKVVIADVQDDLAAALCKEL 60

Query: 61  DD-TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           DD T+ DVSYFHCDVTDE+DISNAVDFAV+KYGKLDIMFNNAGIRG+VTATT+ TDMNDF
Sbjct: 61  DDATNHDVSYFHCDVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFGTFMGAKHAARVMVPAK+GCI+FTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS D EQKKAME  I LSGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDAEQKKAMETLIYLSGNLKGAMMDVEDIAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLN+V+DGGFSLTNPSFEL+ KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

BLAST of HG10020785 vs. ExPASy TrEMBL
Match: A0A0A0LTA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.4e-132
Identity = 245/279 (87.81%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVS+SISKRLAGKVALITGGASGIGESTVRLF+ENGAKVV+ADVQD+L A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFK 120
           DDT  +VSYFHCDVTDE+DISNAVD+AVEKYGKLDIMFNNAGIRG+V +TTLT DMNDF+
Sbjct: 61  DDTGFNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGSTTLTADMNDFR 120

Query: 121 KVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
           KVFDVNVFG+FMGAKHAARVM PAKTGCI+FTSSMASVICSGN PAY+ASKHAI+GLMKT
Sbjct: 121 KVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMKT 180

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKA 240
           LAVELG  GIRVNAISP+ATVTPML+PS++VE+KKAME FISLSGNL+GAVMEAEDVAKA
Sbjct: 181 LAVELGSQGIRVNAISPYATVTPMLVPSRNVEEKKAMEAFISLSGNLQGAVMEAEDVAKA 240

Query: 241 AMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           A+YLGSDESKYVSGLNLVVDGGFSLTNPSFELNAK Y Q
Sbjct: 241 ALYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKIYAQ 279

BLAST of HG10020785 vs. ExPASy TrEMBL
Match: A0A6J1GSR4 (zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.5e-131
Identity = 245/280 (87.50%), Postives = 265/280 (94.64%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           MSSNGVSA+ISKRLAGKVALITGGASGIG STVRLF++NGAKVVIADVQD+LAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  DD-TSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDF 120
           +D T  DVSYFHCDVT+E+DISNAVDFAV+KYGKLDIMFNNAGIRG+VTA T+ TDMNDF
Sbjct: 61  NDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTAMTMNTDMNDF 120

Query: 121 KKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           KKVFDVNVFGTFMGAKHAARVMVPAK+GCI+FTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAMYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKAYGQ 280
           AA+YL SDESKYVSGLN+V+DGGFSLTNPSFEL+ KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

BLAST of HG10020785 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-62
Identity = 132/283 (46.64%), Postives = 182/283 (64.31%), Query Frame = 0

Query: 1   MSSNGVSASI----SKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAAL 60
           MS+N  S+S     S+RL GKVALITGGA+GIGES VRLF ++GAKV I D+QD+L   +
Sbjct: 1   MSTNTESSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEV 60

Query: 61  CKEL--DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTT 120
           CK L   ++     + H DV  E DISNAVDFAV+ +G LDI+ NNAG+ G         
Sbjct: 61  CKSLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNY 120

Query: 121 DMNDFKKVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAI 180
            +++F+  FDVNV G F+  KHAARVM+P K G I+   S+  V+      +Y  SKHA+
Sbjct: 121 SLSEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAV 180

Query: 181 VGLMKTLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKK-----AMEGFISLSGNLKG 240
           +GL +++A ELG HGIRVN +SP+A  T + L     E++          F + + NLKG
Sbjct: 181 LGLTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKG 240

Query: 241 AVMEAEDVAKAAMYLGSDESKYVSGLNLVVDGGFSLTNPSFEL 273
             +  +DVA A ++L SD+S+Y+SG NL++DGGF+ TN SF++
Sbjct: 241 VELTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKV 283

BLAST of HG10020785 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.3e-58
Identity = 134/264 (50.76%), Postives = 170/264 (64.39%), Query Frame = 0

Query: 12  KRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDD--TSGDVSY 71
           KRL GKVA+ITGGA GIG++TV LF  +GA VVIADV +   ++L K L    TS  V++
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 72  FHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVT--ATTLTTDMNDFKKVFDVNV 131
             CDV+ EAD+ N V+  V +YG+LDI+FNNAG+ G+     + L  D ++F  V  VNV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 132 FGTFMGAKHAARVMVP-AKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELG 191
            G  +G KH AR M+     GCI+ T+S+A V+      AY+ASKHAIVGL K  A ELG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 192 PHGIRVNAISPFATVTPMLL------PSKDVEQK--KAMEGFISLSGNLKGAVMEAEDVA 251
            +GIRVN ISPF   T ML+         DVE    + ME F+    NLKG  + A D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 252 KAAMYLGSDESKYVSGLNLVVDGG 263
           +AA+YL SDESKYV+G NLVVDGG
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGG 293

BLAST of HG10020785 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 3.0e-58
Identity = 126/260 (48.46%), Postives = 170/260 (65.38%), Query Frame = 0

Query: 11  SKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDDTSGDVSYF 70
           SK+L GKVALITGGASG+G++T   FL +GA+VVIAD+  E      KEL     +  + 
Sbjct: 38  SKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKEL---GSEAEFV 97

Query: 71  HCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLT-TDMNDFKKVFDVNVFG 130
            CDVT EADI+ AV+  VE+YGKLD+M+NNAGI G +T  +++  DM +F++V  +NVFG
Sbjct: 98  RCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFG 157

Query: 131 TFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHG 190
              G KHAA+ M+PA++GCI+ TSS+A V       +Y+ SK    G++K+ A EL  HG
Sbjct: 158 VVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHG 217

Query: 191 IRVNAISPFATVTPMLLP-----SKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKAAMYL 250
           +R+N ISP    TP+ L         V ++K  E  +   G LKGA  E  DVAKAA+YL
Sbjct: 218 VRINCISPGTVATPLTLSYLQKVFPKVSEEKLRE-TVKGMGELKGAECEEADVAKAALYL 277

Query: 251 GSDESKYVSGLNLVVDGGFS 265
            S++ KYV+G NLVVDGG +
Sbjct: 278 ASNDGKYVTGHNLVVDGGMT 293

BLAST of HG10020785 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.2e-56
Identity = 121/258 (46.90%), Postives = 168/258 (65.12%), Query Frame = 0

Query: 12  KRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKELDDTSGDVSYFH 71
           KRL GK+ +ITGGASGIG  +VRLF E+GA+VVI DVQDEL   +   + +     SY+H
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDK--ASYYH 63

Query: 72  CDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFKKVFDVNVFGTF 131
           CDVT+E ++ NAV F VEKYGKLD++F+NAG+      + L  ++N+  +   +N+ GT 
Sbjct: 64  CDVTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTA 123

Query: 132 MGAKHAARVMV-PAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGI 191
              KHAAR MV     G I+ T+S+A+ I       Y+ SKH ++GL+K+ +  LG +GI
Sbjct: 124 AFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGI 183

Query: 192 RVNAISPFATVTPMLLPSKDVEQKKAMEGFISLSGNLKGAVMEAEDVAKAAMYLGSDESK 251
           RVN ++PF   TP++     +E    +E   S S NLKG V++A  VA+AA++L SDES 
Sbjct: 184 RVNGVAPFGVATPLVCNGFKME-PNVVEQNTSASANLKGIVLKARHVAEAALFLASDESA 243

Query: 252 YVSGLNLVVDGGFSLTNP 269
           YVSG NL VDGG+S+  P
Sbjct: 244 YVSGQNLAVDGGYSVVKP 257

BLAST of HG10020785 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.2e-56
Identity = 126/269 (46.84%), Postives = 173/269 (64.31%), Query Frame = 0

Query: 1   MSSNGVSASISKRLAGKVALITGGASGIGESTVRLFLENGAKVVIADVQDELAAALCKEL 60
           +SS+  S S S++L GKVALITGGASGIG++T   F+ +GAKV+IAD+Q ++     +EL
Sbjct: 65  ISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQEL 124

Query: 61  DDTSGDVSYFHCDVTDEADISNAVDFAVEKYGKLDIMFNNAGIRGNVTATTLTTDMNDFK 120
             +    +YF CDVT E+DI+NAVDFAV  + KLDIM+NNAGI      + +  D+N F 
Sbjct: 125 GPS---CAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFD 184

Query: 121 KVFDVNVFGTFMGAKHAARVMVPAKTGCIMFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
           KV + NV G   G KHAARVM+P  +G I+   S+  ++       YS SK A++G++++
Sbjct: 185 KVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRS 244

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPS-----KDVEQKKAMEGFISLSGNLKGAVMEAE 240
            A EL  H IRVN ISPFA  T  ++         V+  + ++  +  +G L G V E  
Sbjct: 245 TASELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQ-IVQSTGVLNGEVCEPT 304

Query: 241 DVAKAAMYLGSDESKYVSGLNLVVDGGFS 265
           DVA AA+YL SD+SKYV+G NLVVDGGF+
Sbjct: 305 DVANAAVYLASDDSKYVNGHNLVVDGGFT 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437356.11.2e-13389.29PREDICTED: zerumbone synthase [Cucumis melo] >TYJ99091.1 zerumbone synthase [Cuc... [more]
XP_022994653.15.9e-13388.93zerumbone synthase-like [Cucurbita maxima][more]
XP_004152436.12.9e-13287.81zerumbone synthase [Cucumis sativus] >KGN64239.1 hypothetical protein Csa_014346... [more]
KAG6573007.12.9e-13288.21Zerumbone synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023542445.11.1e-13187.86zerumbone synthase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F1SWA07.3e-7052.43Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL73.4e-6751.13Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
A3F5F01.3e-6649.08Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
Q94KL84.2e-6548.34Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
Q7FAE19.3e-6550.19Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BK375.7e-13489.29Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0... [more]
A0A1S3AUF25.7e-13489.29zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1[more]
A0A6J1K5R52.8e-13388.93zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1[more]
A0A0A0LTA01.4e-13287.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1[more]
A0A6J1GSR44.5e-13187.50zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52340.11.2e-6246.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.12.3e-5850.76NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.13.0e-5848.46NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.11.2e-5646.90NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.11.2e-5646.84NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 18..35
score: 49.15
coord: 92..103
score: 58.12
coord: 141..157
score: 28.58
coord: 167..186
score: 45.15
coord: 227..247
score: 28.74
coord: 188..205
score: 42.36
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 92..103
score: 51.1
coord: 147..155
score: 36.85
coord: 167..186
score: 40.72
NoneNo IPR availableGENE3D3.40.50.720coord: 3..277
e-value: 5.0E-83
score: 280.3
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 23..264
e-value: 2.9E-66
score: 223.4
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 20..204
e-value: 4.8E-20
score: 69.5
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 14..267
e-value: 5.1E-23
score: 79.1
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 8..272
NoneNo IPR availablePANTHERPTHR43180:SF71SECOISOLARICIRESINOL DEHYDROGENASE-LIKEcoord: 8..272
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 154..182
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 16..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020785.1HG10020785.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity