HG10020705 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020705
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionE3 ubiquitin-protein ligase MBR2 isoform X2
LocationChr05: 1684845 .. 1686898 (+)
RNA-Seq ExpressionHG10020705
SyntenyHG10020705
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTATTGCTACACACACTTCCACAGTTGGTGAACACATGAAATTCAGAAAACCCAAGAGCCATTTGAACCATTTGAACAAACCCATTTCAGAATCAGATCCAAACCCATCATCAATCCCTTCTATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCTACTTTTTCCAATAATACAACTAGCGGTGGAAATGAATCTTTACCCTCTTCCATTATCACCAACAGGAAGAAGAACAACTTTTCTTCTGCAACTCTCAGGGGACTCGGTTGTACCACCGCCGCTTCTCAGCAGGTATCTGTCCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAGACGAAGAAAAAAAAGCAGAAGAATTCCAAGAACAAAACCCAACAAGGAATTGTTGATGCCTCTCATTTTCACCCTAATTCGAATATCAACTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTGCTGATGCGGCTGCTTCTGTGGATTGTGTTGTTGCTAGAAGACATGCTTCTGGAAGGGGGAAAATAGATTTGGAGAAGATAACTCAGAGGGAGGTACTTACTTTTTTCTACTTTCTTTTGAATTTATCTTTGAATTTCTTTCTTCTGTGTCGATGTGTCGTTCTGTTGTTGTTGTTGTTGTTAAGCATTCGTGTCGATTTCGTAATTAAAGAAGAATTTGGGTGTTTATTTTATTTCAGCTTTTGTCATTTTATTATTATCACTACTTCAGATGTTGGTTTCTTCTGTTTTTTTTAATCGAACTTTATCGTCTTTGTTTCTTTTCTTCAATAGGGTTCTTCTAAATCAGTCTGTCCCTGTTCGACTTCCATTCTTGGAACTTGTTTGATTTCATTCTTCTTCGTAAATTTGAGTATTTAATGCAAATGGGTATGCATTAGATTTGGAATTTGTCTGAATGGAGTCTCTTCTTTGGGACTATAAAGATGAAAAATCACTTGTTCATCGTTCATTATCGATAACAAACTCTGTTTAATGCGTTTTAACTTCTCTGCTTTTTCCATTACAGCGTTCTTGTTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTATTTTTGGATTCTGATTCTGATCTTCCAACTGCTCGATCATTGGAACTGTCTCGGAGTCGGTATTATCGCCATGTTCGTCATCCATCCCCCGATGGCCTTGCTGAGGTGAGCCATCTTTCGTATTTGTATTCAGTTTCATAAATACAAGCTTTAGTTGGAACGACTAGGTGGTTCTACAACTCTGTCTCTGAGTTCTAAAAATATAAGATTCAGTTGAGTTCTTAATGGCTGTTTTTTCTTTTAGTTTTCTTAACACATTAGTTGGAAATGTTTTCACATGATGTTGTTCTTTTTATGTACTACTTAAAAGAACCTCTGTTTCACAATGATCATATGAGACTGATTTGCTCATTTGATATTACATTCACATTATTTAGCAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATCTACACGACCAATTTAGGGAATTACGACTTGACGTCGATAACATGTCGTATGAGGTTCGTAACTTTATCCAGTTTCTCCTTCTTGACACAATGTGATACTCACAGCTCTTCCTTATTTTGGAAACAGGAGCTGCTTGAACTCGGCGAAAGGATCGGCCATGTCAGTACCGGGCTGAAAGAAGACGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTGAATGAGCTAACAACTCATTTGTTATCTCAAATGGATAGGAAGTGCAGCATCTGTCAGGTGATGTAGAAGAGTTCAGTTTTGCTGGCTTTGATTAGCATTTGTGTATGTAATCACATGCTTAACATCTACAATTATTTTGTACAAGCAGGAGGACTATGAACCAGATGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTACCATATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCTGTCTGTAAGACAGCAGCGGTGGGCCGAGGTTGA

mRNA sequence

ATGCCTATTGCTACACACACTTCCACAGTTGGTGAACACATGAAATTCAGAAAACCCAAGAGCCATTTGAACCATTTGAACAAACCCATTTCAGAATCAGATCCAAACCCATCATCAATCCCTTCTATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCTACTTTTTCCAATAATACAACTAGCGGTGGAAATGAATCTTTACCCTCTTCCATTATCACCAACAGGAAGAAGAACAACTTTTCTTCTGCAACTCTCAGGGGACTCGGTTGTACCACCGCCGCTTCTCAGCAGGTATCTGTCCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAGACGAAGAAAAAAAAGCAGAAGAATTCCAAGAACAAAACCCAACAAGGAATTGTTGATGCCTCTCATTTTCACCCTAATTCGAATATCAACTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTGCTGATGCGGCTGCTTCTGTGGATTGTCGTTCTTGTTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTATTTTTGGATTCTGATTCTGATCTTCCAACTGCTCGATCATTGGAACTGTCTCGGAGTCGGTATTATCGCCATGTTCGTCATCCATCCCCCGATGGCCTTGCTGAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATCTACACGACCAATTTAGGGAATTACGACTTGACGTCGATAACATGTCGTATGAGGAGCTGCTTGAACTCGGCGAAAGGATCGGCCATGTCAGTACCGGGCTGAAAGAAGACGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTGAATGAGCTAACAACTCATTTGTTATCTCAAATGGATAGGAAGTGCAGCATCTGTCAGGAGGACTATGAACCAGATGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTACCATATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCTGTCTGTAAGACAGCAGCGGTGGGCCGAGGTTGA

Coding sequence (CDS)

ATGCCTATTGCTACACACACTTCCACAGTTGGTGAACACATGAAATTCAGAAAACCCAAGAGCCATTTGAACCATTTGAACAAACCCATTTCAGAATCAGATCCAAACCCATCATCAATCCCTTCTATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCTACTTTTTCCAATAATACAACTAGCGGTGGAAATGAATCTTTACCCTCTTCCATTATCACCAACAGGAAGAAGAACAACTTTTCTTCTGCAACTCTCAGGGGACTCGGTTGTACCACCGCCGCTTCTCAGCAGGTATCTGTCCCGGCTGTAATTCGAACTTCAGCGGATTGGGAAAAGAAGAAGACGAAGAAAAAAAAGCAGAAGAATTCCAAGAACAAAACCCAACAAGGAATTGTTGATGCCTCTCATTTTCACCCTAATTCGAATATCAACTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTGCTGATGCGGCTGCTTCTGTGGATTGTCGTTCTTGTTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTATTTTTGGATTCTGATTCTGATCTTCCAACTGCTCGATCATTGGAACTGTCTCGGAGTCGGTATTATCGCCATGTTCGTCATCCATCCCCCGATGGCCTTGCTGAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATCTACACGACCAATTTAGGGAATTACGACTTGACGTCGATAACATGTCGTATGAGGAGCTGCTTGAACTCGGCGAAAGGATCGGCCATGTCAGTACCGGGCTGAAAGAAGACGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTGAATGAGCTAACAACTCATTTGTTATCTCAAATGGATAGGAAGTGCAGCATCTGTCAGGAGGACTATGAACCAGATGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTACCATATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCTGTCTGTAAGACAGCAGCGGTGGGCCGAGGTTGA

Protein sequence

MPIATHTSTVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSGGNESLPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQKNSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDCRSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTCPVCKTAAVGRG
Homology
BLAST of HG10020705 vs. NCBI nr
Match: XP_038894454.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida])

HSP 1 Score: 677.6 bits (1747), Expect = 6.0e-191
Identity = 344/379 (90.77%), Postives = 351/379 (92.61%), Query Frame = 0

Query: 1   MPIATHTSTVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60
           MP+ THTSTVGEHMK R+PKS LNHLN+PISESDPNPSSIPSIIQSTRCKSTISSLLLST
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60

Query: 61  FSNNTTSGGNESLPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120
           FSNNTTSGGNE+LPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE+KK
Sbjct: 61  FSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWERKK 120

Query: 121 TKKKKQKNSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC-- 180
           T+KKKQKNSKNKTQQGIVDASHF PNSNINSASCLDAQDVWCGPGIGFSADAAASVDC  
Sbjct: 121 TRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 --------------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240
                               RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300
           RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK
Sbjct: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300

Query: 301 EDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQ 358
           EDEI RCIRKMKPLVVNEL THLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQ
Sbjct: 301 EDEIVRCIRKMKPLVVNELATHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQ 360

BLAST of HG10020705 vs. NCBI nr
Match: XP_038894453.1 (E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida])

HSP 1 Score: 672.9 bits (1735), Expect = 1.5e-189
Identity = 344/380 (90.53%), Postives = 351/380 (92.37%), Query Frame = 0

Query: 1   MPIATHTSTVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60
           MP+ THTSTVGEHMK R+PKS LNHLN+PISESDPNPSSIPSIIQSTRCKSTISSLLLST
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60

Query: 61  FSNNTTSGGNESLPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120
           FSNNTTSGGNE+LPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE+KK
Sbjct: 61  FSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWERKK 120

Query: 121 TKKKKQKNSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC-- 180
           T+KKKQKNSKNKTQQGIVDASHF PNSNINSASCLDAQDVWCGPGIGFSADAAASVDC  
Sbjct: 121 TRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 --------------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240
                               RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAE-IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           RHPSPDGLAE IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 RHPSPDGLAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK 358
           KEDEI RCIRKMKPLVVNEL THLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK
Sbjct: 301 KEDEIVRCIRKMKPLVVNELATHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK 360

BLAST of HG10020705 vs. NCBI nr
Match: XP_004137488.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus])

HSP 1 Score: 643.3 bits (1658), Expect = 1.3e-180
Identity = 331/372 (88.98%), Postives = 343/372 (92.20%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQGIVDASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDS++PTARSLELSRSRYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHVRHPSPDG 244

Query: 249 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 308
           LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK+DEIGRC
Sbjct: 245 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKDDEIGRC 304

Query: 309 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 358
           IRKMKP VVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT
Sbjct: 305 IRKMKPHVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 364

BLAST of HG10020705 vs. NCBI nr
Match: XP_011650643.1 (E3 ubiquitin-protein ligase MBR2 isoform X1 [Cucumis sativus] >KAE8652553.1 hypothetical protein Csa_013283 [Cucumis sativus])

HSP 1 Score: 638.6 bits (1646), Expect = 3.1e-179
Identity = 331/373 (88.74%), Postives = 343/373 (91.96%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQGIVDASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDS++PTARSLELSRSRYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHVRHPSPDG 244

Query: 249 LAE-IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGR 308
           LAE IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK+DEIGR
Sbjct: 245 LAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKDDEIGR 304

Query: 309 CIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN 358
           CIRKMKP VVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN
Sbjct: 305 CIRKMKPHVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN 364

BLAST of HG10020705 vs. NCBI nr
Match: XP_008461518.1 (PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis melo] >TYJ99014.1 E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 635.6 bits (1638), Expect = 2.6e-178
Identity = 328/372 (88.17%), Postives = 340/372 (91.40%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE KKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQG++DASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDSDL TARSLELSR+RYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHVRHPSPDG 244

Query: 249 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 308
           LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC
Sbjct: 245 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 304

Query: 309 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 358
           IRKMKPLVVNELTTHLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI CIKQWLAQKNT
Sbjct: 305 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCIKQWLAQKNT 364

BLAST of HG10020705 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-29
Identity = 67/150 (44.67%), Postives = 90/150 (60.00%), Query Frame = 0

Query: 205 RSLELSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELL 264
           R+  +S  R   H      +   E  M    L+  G  D+HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 265 ELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGK 324
            LGERIG VSTGL E+ I + +++ K    +  +  L   ++  C ICQE+Y   D +G 
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIE-PCCICQEEYVEGDNLGT 672

Query: 325 LECGHSYHIHCIKQWLAQKNTCPVCKTAAV 355
           L+CGH +H  CIKQW+  KN CP+CKT A+
Sbjct: 673 LKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701

BLAST of HG10020705 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 2.3e-28
Identity = 59/122 (48.36%), Postives = 85/122 (69.67%), Query Frame = 0

Query: 235 SLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVV 294
           S+  GG  D+HD+ R++RLD+DNMSYEELL L ERIG+VSTGL E+E+ + +++ K    
Sbjct: 551 SIFYGG-VDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRK---- 610

Query: 295 NELTTHLL--SQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTCPVCKTA 354
              ++  L  S  +  C ICQE+Y   D++G L+CGH +H+ C++QWL  KNTCP+CK  
Sbjct: 611 --FSSWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNT 665

BLAST of HG10020705 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-27
Identity = 60/130 (46.15%), Postives = 84/130 (64.62%), Query Frame = 0

Query: 226 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 285
           L ++M+   S+L  G    HD++R++RLDVDNMSYEELL L ERIG V TG+ E+ I   
Sbjct: 560 LEDVMLLNHSVLFDGATG-HDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNR 619

Query: 286 IRKMKPLVVNELTTHLLSQMDRK-CSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN 345
           +++ K        ++  S  D + C +CQE+Y   ++MG LECGH +H  CIK+WL QKN
Sbjct: 620 LKQRK------YKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKN 679

Query: 346 TCPVCKTAAV 355
            CP+CKT  +
Sbjct: 680 LCPICKTTGL 682

BLAST of HG10020705 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-27
Identity = 61/128 (47.66%), Postives = 83/128 (64.84%), Query Frame = 0

Query: 228 EIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIR 287
           E  M    L+  G  ++HD+ R++RLDVDNMSYEELL LGERIG VSTGL E+ I + ++
Sbjct: 542 EDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMK 601

Query: 288 KMKPLVVNELTTHLLSQMDRK-CSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTC 347
           + K       ++   S  D + C +CQE+Y   D++G L CGH +H  C+KQWL  KN C
Sbjct: 602 QHK-----HTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLC 661

Query: 348 PVCKTAAV 355
           P+CKT A+
Sbjct: 662 PICKTVAL 664

BLAST of HG10020705 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.6e-22
Identity = 62/169 (36.69%), Postives = 94/169 (55.62%), Query Frame = 0

Query: 192 LLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFREL 251
           +LF +S+          L+    +R++R   P+  A IM F     +    D H   R++
Sbjct: 355 VLFSNSERTFGPPHHPLLANPVNHRNIRILPPEH-ATIMDFSRLYEVSNVVDEH---RDM 414

Query: 252 RLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRK--- 311
           RLD+D+M+YEELL L E+IG V+TGL +  I   ++    +  +   ++  S+   +   
Sbjct: 415 RLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMSNKSSESSMENDA 474

Query: 312 CSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTCPVCKTAAVGRG 358
           C ICQE+Y+  + +G L+CGH YH  CIKQWL  KN CP+CKT A+  G
Sbjct: 475 CIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTALSTG 519

BLAST of HG10020705 vs. ExPASy TrEMBL
Match: A0A0A0LQF5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.1e-181
Identity = 331/372 (88.98%), Postives = 343/372 (92.20%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQGIVDASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDS++PTARSLELSRSRYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHVRHPSPDG 244

Query: 249 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 308
           LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK+DEIGRC
Sbjct: 245 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKDDEIGRC 304

Query: 309 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 358
           IRKMKP VVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT
Sbjct: 305 IRKMKPHVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 364

BLAST of HG10020705 vs. ExPASy TrEMBL
Match: A0A5D3BIX1 (E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002180 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.3e-178
Identity = 328/372 (88.17%), Postives = 340/372 (91.40%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE KKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQG++DASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDSDL TARSLELSR+RYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHVRHPSPDG 244

Query: 249 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 308
           LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC
Sbjct: 245 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 304

Query: 309 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 358
           IRKMKPLVVNELTTHLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI CIKQWLAQKNT
Sbjct: 305 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCIKQWLAQKNT 364

BLAST of HG10020705 vs. ExPASy TrEMBL
Match: A0A1S3CET5 (E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500009 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.3e-178
Identity = 328/372 (88.17%), Postives = 340/372 (91.40%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE KKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQG++DASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDSDL TARSLELSR+RYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHVRHPSPDG 244

Query: 249 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 308
           LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC
Sbjct: 245 LAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 304

Query: 309 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNT 358
           IRKMKPLVVNELTTHLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI CIKQWLAQKNT
Sbjct: 305 IRKMKPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCIKQWLAQKNT 364

BLAST of HG10020705 vs. ExPASy TrEMBL
Match: A0A1S3CER0 (E3 ubiquitin-protein ligase MBR2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500009 PE=4 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.1e-177
Identity = 328/373 (87.94%), Postives = 340/373 (91.15%), Query Frame = 0

Query: 9   TVGEHMKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSG 68
           TVGEHMK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG
Sbjct: 5   TVGEHMKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSG 64

Query: 69  GNESLPSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQK 128
            NESLPSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE KKT+KKKQK
Sbjct: 65  PNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKKTRKKKQK 124

Query: 129 NSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC--------- 188
           +SKNKTQQG++DASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC         
Sbjct: 125 SSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASG 184

Query: 189 -------------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDG 248
                        RSCLGRRTV+PETLLFLDSDSDL TARSLELSR+RYYRHVRHPSPDG
Sbjct: 185 RGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHVRHPSPDG 244

Query: 249 LAE-IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGR 308
           LAE IMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGR
Sbjct: 245 LAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGR 304

Query: 309 CIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN 358
           CIRKMKPLVVNELTTHLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI CIKQWLAQKN
Sbjct: 305 CIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCIKQWLAQKN 364

BLAST of HG10020705 vs. ExPASy TrEMBL
Match: A0A1S3CEY2 (E3 ubiquitin-protein ligase MBR2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500009 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 7.2e-174
Identity = 323/368 (87.77%), Postives = 335/368 (91.03%), Query Frame = 0

Query: 14  MKFRKPKSHLNHLNKPISESDPNPSSIPSIIQSTRCKSTISSLLLSTFSNNTTSGGNESL 73
           MK R+P+S L+HLN+PISESDPNP SIPSIIQSTRCKSTISSLLLSTFSNNTTSG NESL
Sbjct: 1   MKLRRPRSDLSHLNQPISESDPNP-SIPSIIQSTRCKSTISSLLLSTFSNNTTSGPNESL 60

Query: 74  PSSII-TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKKTKKKKQKNSKNK 133
           PSSII TNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE KKT+KKKQK+SKNK
Sbjct: 61  PSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKKTRKKKQKSSKNK 120

Query: 134 TQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADAAASVDC-------------- 193
           TQQG++DASHF PNS++NSASCLDAQDVWCGPGIGFSADAAASVDC              
Sbjct: 121 TQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRHASGRGKID 180

Query: 194 --------RSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHVRHPSPDGLAE-I 253
                   RSCLGRRTV+PETLLFLDSDSDL TARSLELSR+RYYRHVRHPSPDGLAE I
Sbjct: 181 LEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHVRHPSPDGLAEQI 240

Query: 254 MMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKM 313
           MMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKM
Sbjct: 241 MMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKM 300

Query: 314 KPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNTCPVC 358
           KPLVVNELTTHLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI CIKQWLAQKNTCPVC
Sbjct: 301 KPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHIQCIKQWLAQKNTCPVC 360

BLAST of HG10020705 vs. TAIR 10
Match: AT1G73760.1 (RING/U-box superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.5e-73
Identity = 171/396 (43.18%), Postives = 228/396 (57.58%), Query Frame = 0

Query: 2   PIATHTSTVGEHMKFRKPKSHLNHLNKPISESD----PNPSSIPSIIQSTRCKSTISSLL 61
           P ++ ++T+G+HM+ ++P++H N    PIS +D    P PS +         KS +SS  
Sbjct: 7   PSSSSSTTIGQHMRLQRPRNHRN--LPPISTADEPLIPKPSRV--------SKSAMSSFF 66

Query: 62  LSTFSNNTTSGGNESLPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWE 121
           L              LP    T +KK N  +A+ RGLGCTT+ASQQVSVPAVIR+SADW+
Sbjct: 67  L--------------LPE---TTKKKPN-GTASFRGLGCTTSASQQVSVPAVIRSSADWD 126

Query: 122 KKKTK-KKKQKNSKNKTQQGIVDAS--HFHPNSNINSASCLDAQDVWCGPGIGFSADA-- 181
               K KK +K +KNK        S       S  +S +C    DVWCGPG+GFS DA  
Sbjct: 127 ASNFKIKKTKKKNKNKGSSSYNGGSIKILSEASTSSSVACAAIPDVWCGPGVGFSTDAVV 186

Query: 182 AASVD--------------------------------CRSCLGRRTVNPETLLFLDSDSD 241
             S+D                                  S L RR++N E+  + DSDS 
Sbjct: 187 GGSIDTVVSDPPRRNIPVRRKIDGDKTNSNSNNHREGSSSLLPRRSLNQESNPYFDSDSS 246

Query: 242 LPTARSLELSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSY 301
             T+R+ +    RY+RH+R P PDGLAE+MM Q+  +MGG     DQFR++RL+VDNM+Y
Sbjct: 247 FLTSRAEQ--TDRYHRHLRLPYPDGLAEMMMMQNGFVMGGVLSSFDQFRDMRLNVDNMTY 306

Query: 302 EELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDD 357
           E+LLELGERIGHV+TGL E +I  C+RK+KP    + TT      DRKC ICQ++YE  D
Sbjct: 307 EQLLELGERIGHVNTGLTEKQIKSCLRKVKP--CRQDTT----VADRKCIICQDEYEAKD 366

BLAST of HG10020705 vs. TAIR 10
Match: AT1G17970.1 (RING/U-box superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 158/387 (40.83%), Postives = 219/387 (56.59%), Query Frame = 0

Query: 7   TSTVGEHMKFRKPKSH-LNHLNKPISESDPNPSSIP-SIIQSTR-CKSTISSLLLSTFSN 66
           ++T+GEH++ R+ ++  + HL+   ++ DP  S +   I Q  R C S +SS     F  
Sbjct: 3   STTIGEHIRLRRARNQTIRHLH--AADDDPPLSHVVLPISQPNRFCNSAMSS-----FFP 62

Query: 67  NTTSGGNESLPSSIITNRKKNNFSSATLRGLGC---TTAASQQVSVPAVIRTSADWEKKK 126
             TS  NES         +K  + +++ RG+GC     AA+Q+VSVP+VIR SAD + + 
Sbjct: 63  LPTSSSNES--------TRKKPYQTSSFRGMGCYAAAAAAAQEVSVPSVIRYSADLDARI 122

Query: 127 TKKKKQKNSKNKTQQGIVDASHFHPNSNINSASCLDAQDVWCGPGIGFSADA--AASVD- 186
            K KK+K  K+K ++     S+   +  I S    D  DVWC PG+GFS DA    SVD 
Sbjct: 123 RKDKKKKKHKHKKKKKKNKGSYEDGSIRILSEEARDVIDVWCRPGLGFSTDAVIGRSVDP 182

Query: 187 ------------------------CRSCLGRRTVNPETLL--FLDSDSDLPTARSLE--L 246
                                     S L  R +N ET      +SDS   T+   E  +
Sbjct: 183 PRGRNIPSSRRKIDVDNNNYNHTLGSSVLPIRFLNQETHSHDIFNSDSTFVTSSRAEPTM 242

Query: 247 SRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGER 306
             SR   H+    PD L E+ M Q+  +MG   D  D + ELRLDVD+MSYE+LLELG+R
Sbjct: 243 LSSRCRGHLPRSYPDDLTEMRMLQNGFVMGRITDSRDNYHELRLDVDSMSYEQLLELGDR 302

Query: 307 IGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGH 357
           IG+V+TGLKE EI RC+ K+KP V + L       +DRKCSICQ++YE +DE+G+L CGH
Sbjct: 303 IGYVNTGLKESEIHRCLGKIKPSVSHTL-------VDRKCSICQDEYEREDEVGELNCGH 362

BLAST of HG10020705 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.1e-32
Identity = 62/138 (44.93%), Postives = 92/138 (66.67%), Query Frame = 0

Query: 217 HVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTG 276
           H+R      L ++M+   S+++G   D+HD++R++RLDVDNM+YEELL L ERIG V TG
Sbjct: 506 HMRRAGNLRLEDVMLLNQSIMLGAA-DIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTG 565

Query: 277 LKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 336
           L E+ I   +++ K       ++   SQ    C +CQE+Y+ ++E+G+LECGH +H  CI
Sbjct: 566 LNEETISNRLKQQK-----YKSSTRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQCI 625

Query: 337 KQWLAQKNTCPVCKTAAV 355
           K+WL QKN CP+CKT  +
Sbjct: 626 KEWLKQKNLCPICKTTGL 637

BLAST of HG10020705 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 8.0e-32
Identity = 63/131 (48.09%), Postives = 92/131 (70.23%), Query Frame = 0

Query: 228 EIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIR 287
           ++++ ++ LL+GG    HDQ R++RLD+DNMSYEELL L ERIG VST L E+ I +C++
Sbjct: 363 DLLIMETGLLLGG-LSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISKCLK 422

Query: 288 ----KMKPLVVNELT-THLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQ 347
               +MKPL    +T +   ++ D KCSICQE+Y   DE+G+L C H+YH+ C+++WL  
Sbjct: 423 TSIYQMKPLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEWLRI 482

Query: 348 KNTCPVCKTAA 354
           K+ CP+CK  A
Sbjct: 483 KSWCPICKATA 492

BLAST of HG10020705 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-30
Identity = 67/150 (44.67%), Postives = 90/150 (60.00%), Query Frame = 0

Query: 205 RSLELSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELL 264
           R+  +S  R   H      +   E  M    L+  G  D+HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 265 ELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEPDDEMGK 324
            LGERIG VSTGL E+ I + +++ K    +  +  L   ++  C ICQE+Y   D +G 
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIE-PCCICQEEYVEGDNLGT 672

Query: 325 LECGHSYHIHCIKQWLAQKNTCPVCKTAAV 355
           L+CGH +H  CIKQW+  KN CP+CKT A+
Sbjct: 673 LKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894454.16.0e-19190.77E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida][more]
XP_038894453.11.5e-18990.53E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida][more]
XP_004137488.11.3e-18088.98E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus][more]
XP_011650643.13.1e-17988.74E3 ubiquitin-protein ligase MBR2 isoform X1 [Cucumis sativus] >KAE8652553.1 hypo... [more]
XP_008461518.12.6e-17888.17PREDICTED: E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis melo] >TYJ99014.... [more]
Match NameE-valueIdentityDescription
Q9ZQF91.6e-2944.67E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q7XTV72.3e-2848.36Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9FMM41.2e-2746.15Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
O495001.5e-2747.66E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q5QLR55.6e-2236.69Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A0A0LQF56.1e-18188.98RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 P... [more]
A0A5D3BIX11.3e-17888.17E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CET51.3e-17888.17E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500... [more]
A0A1S3CER03.1e-17787.94E3 ubiquitin-protein ligase MBR2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500... [more]
A0A1S3CEY27.2e-17487.77E3 ubiquitin-protein ligase MBR2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500... [more]
Match NameE-valueIdentityDescription
AT1G73760.15.5e-7343.18RING/U-box superfamily protein [more]
AT1G17970.11.6e-6140.83RING/U-box superfamily protein [more]
AT1G45180.12.1e-3244.93RING/U-box superfamily protein [more]
AT4G31450.18.0e-3248.09RING/U-box superfamily protein [more]
AT2G15530.11.1e-3044.67RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 309..349
e-value: 1.9E-7
score: 40.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 308..350
e-value: 2.9E-13
score: 50.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 309..350
score: 12.799091
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 287..354
e-value: 1.8E-18
score: 67.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..134
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 180..352
NoneNo IPR availablePANTHERPTHR22937:SF125C3HC4-TYPE RING ZINC FINGER PROTEINcoord: 6..178
NoneNo IPR availablePANTHERPTHR22937:SF125C3HC4-TYPE RING ZINC FINGER PROTEINcoord: 180..352
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 6..178
NoneNo IPR availableCDDcd16481RING-H2_TTC3coord: 308..350
e-value: 1.99858E-17
score: 73.1501
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 299..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020705.1HG10020705.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity