HG10020489 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020489
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionE3 ubiquitin-protein ligase SGR9, amyloplastic
LocationChr04: 32263052 .. 32263876 (-)
RNA-Seq ExpressionHG10020489
SyntenyHG10020489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGTGTCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATTCACCACCACCGCCGCCGCCTCACCTTCATCCTCTCTTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCCTTGCCCGCCTCCTCCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGTCACCATCCAAGCTCCTCCCTTACCACCCTTCCATCGCCGCCATCTCCCCTCAAGACCTCGATGCCGCCGTCCTCCTCCTCCTCCTCTGTGAGGTTCGGCAACACAATCCAGCTGCCCTACGAACTCCGATCACCAAATGGCGCGCGACCCTCTGTAGAATCTACTCCGATTCAATATTGACGATCTCCGGTGTCGCCACCGGCGGGGGCGGGGCTTTGATTCCGTACATTGAGACGGTGGCGAGGTGTTGGAAATTCGTGGGGTTTGTTGGGAGTTGCGGAGGGAAGGCCCGGAGAGAGGTGGCGGCTTCGCCTATGGCGGTGGTGGAGCTTCCGTCGGTGGCGGTGGGTGGTGGCGGTGCAGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGGGAGAAGGGAGAGACGCGTGTAAATTGCCTTGTGACCACTTATTCCATTGGCTCTGTATTTTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGTAGGTTTCAGCTTCCCACTGATGATATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAAGTGGGCTCCACAATGTGTACATCTTCTGATGGAGATTAA

mRNA sequence

ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGTGTCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATTCACCACCACCGCCGCCGCCTCACCTTCATCCTCTCTTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCCTTGCCCGCCTCCTCCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGTCACCATCCAAGCTCCTCCCTTACCACCCTTCCATCGCCGCCATCTCCCCTCAAGACCTCGATGCCGCCGTCCTCCTCCTCCTCCTCTGTGAGGTTCGGCAACACAATCCAGCTGCCCTACGAACTCCGATCACCAAATGGCGCGCGACCCTCTGTAGAATCTACTCCGATTCAATATTGACGATCTCCGGTGTCGCCACCGGCGGGGGCGGGGCTTTGATTCCGTACATTGAGACGGTGGCGAGGTGTTGGAAATTCGTGGGGTTTGTTGGGAGTTGCGGAGGGAAGGCCCGGAGAGAGGTGGCGGCTTCGCCTATGGCGGTGGTGGAGCTTCCGTCGGTGGCGGTGGGTGGTGGCGGTGCAGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGGGAGAAGGGAGAGACGCGTGTAAATTGCCTTGTGACCACTTATTCCATTGGCTCTGTATTTTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGTAGGTTTCAGCTTCCCACTGATGATATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAAGTGGGCTCCACAATGTGTACATCTTCTGATGGAGATTAA

Coding sequence (CDS)

ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGTGTCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATTCACCACCACCGCCGCCGCCTCACCTTCATCCTCTCTTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCCTTGCCCGCCTCCTCCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGTCACCATCCAAGCTCCTCCCTTACCACCCTTCCATCGCCGCCATCTCCCCTCAAGACCTCGATGCCGCCGTCCTCCTCCTCCTCCTCTGTGAGGTTCGGCAACACAATCCAGCTGCCCTACGAACTCCGATCACCAAATGGCGCGCGACCCTCTGTAGAATCTACTCCGATTCAATATTGACGATCTCCGGTGTCGCCACCGGCGGGGGCGGGGCTTTGATTCCGTACATTGAGACGGTGGCGAGGTGTTGGAAATTCGTGGGGTTTGTTGGGAGTTGCGGAGGGAAGGCCCGGAGAGAGGTGGCGGCTTCGCCTATGGCGGTGGTGGAGCTTCCGTCGGTGGCGGTGGGTGGTGGCGGTGCAGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGGGAGAAGGGAGAGACGCGTGTAAATTGCCTTGTGACCACTTATTCCATTGGCTCTGTATTTTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGTAGGTTTCAGCTTCCCACTGATGATATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAAGTGGGCTCCACAATGTGTACATCTTCTGATGGAGATTAA

Protein sequence

MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLARLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPITKWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASPMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD
Homology
BLAST of HG10020489 vs. NCBI nr
Match: KAA0044054.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa] >TYK25081.1 E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa])

HSP 1 Score: 518.8 bits (1335), Expect = 2.8e-143
Identity = 254/274 (92.70%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAAL+ LSPP L+DLSHSIFS++H HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA
Sbjct: 1   MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT
Sbjct: 61  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRATLCRIYS+S+LTISG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 121 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 180

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           MAVVELPSVAVGGGG  VECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC
Sbjct: 181 MAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+FGEIQRLWEIL KVGSTMCT SDGD
Sbjct: 241 RFQLPTDDVFGEIQRLWEILFKVGSTMCT-SDGD 273

BLAST of HG10020489 vs. NCBI nr
Match: XP_016899723.1 (PREDICTED: E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo])

HSP 1 Score: 518.8 bits (1335), Expect = 2.8e-143
Identity = 254/274 (92.70%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAAL+ LSPP L+DLSHSIFS++H HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA
Sbjct: 11  MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 70

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT
Sbjct: 71  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 130

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRATLCRIYS+S+LTISG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 131 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 190

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           MAVVELPSVAVGGGG  VECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 MAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+FGEIQRLWEIL KVGSTMCT SDGD
Sbjct: 251 RFQLPTDDVFGEIQRLWEILFKVGSTMCT-SDGD 283

BLAST of HG10020489 vs. NCBI nr
Match: XP_004137953.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis sativus] >KGN58913.1 hypothetical protein Csa_002303 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 4.7e-143
Identity = 254/273 (93.04%), Postives = 263/273 (96.34%), Query Frame = 0

Query: 2   AALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR 61
           AAL+ LSPP L+DLSHSIFS+IH HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR
Sbjct: 12  AALATLSPPRLADLSHSIFSEIHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR 71

Query: 62  LLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK 121
            LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK
Sbjct: 72  FLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK 131

Query: 122 WRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASPM 181
           WRATLC+IYSDS+LT+SG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASPM
Sbjct: 132 WRATLCKIYSDSLLTVSGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASPM 191

Query: 182 AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR 241
           AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR
Sbjct: 192 AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR 251

Query: 242 FQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           FQLPTDD+ GEIQRLWEIL KVGSTMCT SDGD
Sbjct: 252 FQLPTDDVLGEIQRLWEILFKVGSTMCT-SDGD 283

BLAST of HG10020489 vs. NCBI nr
Match: XP_038905798.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Benincasa hispida])

HSP 1 Score: 514.6 bits (1324), Expect = 5.2e-142
Identity = 257/274 (93.80%), Postives = 261/274 (95.26%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAALSALSPP LSDLSHSIFSDIH HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA
Sbjct: 11  MAALSALSPPSLSDLSHSIFSDIHLHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 70

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLS LRRLS PFQS SKL P+HPS A ISPQDLDAAVLLLLLCEVRQHNPAALRTPIT
Sbjct: 71  RFLLSTLRRLSCPFQSQSKLFPHHPSTATISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 130

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRATLCRIYSDS+LTISG+ATGGGGALIPYIETV RCWKFVGFVGSCGGKARREVAASP
Sbjct: 131 KWRATLCRIYSDSLLTISGIATGGGGALIPYIETVVRCWKFVGFVGSCGGKARREVAASP 190

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           +AVVELPSVAVGGGGA VECVICKEEM EGRDACKLPCDHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 VAVVELPSVAVGGGGALVECVICKEEMVEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDDIFGEIQRLWEILLKVGSTMCT SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWEILLKVGSTMCT-SDGD 283

BLAST of HG10020489 vs. NCBI nr
Match: XP_022145706.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Momordica charantia])

HSP 1 Score: 468.4 bits (1204), Expect = 4.3e-128
Identity = 231/274 (84.31%), Postives = 250/274 (91.24%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAALSALSP  LSDLSHSIFSDIHH RRRLTFILSSPTLFSLTLRHLNSLSLSHKSLL+A
Sbjct: 40  MAALSALSPLHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 99

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRL  PFQ P+K LP HPS AAI+P+DLDAAVLLLLLCEVRQHNPAAL TP+ 
Sbjct: 100 RFLLSALRRLCHPFQPPAKSLPQHPSTAAITPRDLDAAVLLLLLCEVRQHNPAALETPLP 159

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRA LCRIYSDSIL+ISG+ +GGG  ++PY+ETVARCWKFVGFVGSCGGK +REVAASP
Sbjct: 160 KWRAALCRIYSDSILSISGIISGGGAVMVPYVETVARCWKFVGFVGSCGGKGKREVAASP 219

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           +AVVELPSVA     AAVECVICKEEMGEGRDAC+LPC+HLFHWLCILPWLRKRNTCPCC
Sbjct: 220 VAVVELPSVA---AAAAVECVICKEEMGEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 279

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+F EIQRLWEILLK+GST+ T+SDGD
Sbjct: 280 RFQLPTDDVFAEIQRLWEILLKLGSTI-TTSDGD 309

BLAST of HG10020489 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.8e-66
Identity = 148/274 (54.01%), Postives = 176/274 (64.23%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MA+LSALSP  L++L+HSI S  HHHRRRL  +LSSPTLFSLTLRHL SLSL  K+ L+A
Sbjct: 10  MASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLSLPDKTHLIA 69

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIA-AISPQDLDAAVLLLLLCEVRQHNPAALRTPI 120
             LLS L     P     K   +H S A  +  +DLDA VLLL LCE  Q +P  L    
Sbjct: 70  NHLLSLL----HPLLIHRK---HHSSYAVTMKLRDLDAVVLLLFLCETHQLHPDVLEASA 129

Query: 121 TKWRATLCRIYSDSILT-ISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARR---- 180
             WR  L   YS+++L+  SG+ T   G L+PYIET+ RC +FV  +G      RR    
Sbjct: 130 DNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGYNHLRRRDQKE 189

Query: 181 --EVAASPMAVVELPSVAV----GGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCIL 240
             +V A+  AVV L +V V          VECVICKEEM EGRD C++PC H FHW CIL
Sbjct: 190 GYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPCQHFFHWKCIL 249

Query: 241 PWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLK 263
           PWL K+NTCP CRFQLPTDD+F EIQRLWEIL+K
Sbjct: 250 PWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

BLAST of HG10020489 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-14
Identity = 34/71 (47.89%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 178 ASPMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTC 237
           A+   V  LP   + G  A ++C +C  E  E   A ++PC HLFH  CILPWL K N+C
Sbjct: 54  AAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSC 113

Query: 238 PCCRFQLPTDD 249
           P CR++LPTDD
Sbjct: 114 PLCRYELPTDD 124

BLAST of HG10020489 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-14
Identity = 35/71 (49.30%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 178 ASPMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTC 237
           A+  AV  LP   + G  A ++C +C  E  E   A ++PC HLFH  CILPWL K N+C
Sbjct: 54  AAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSNCILPWLSKTNSC 113

Query: 238 PCCRFQLPTDD 249
           P CR +LPTDD
Sbjct: 114 PLCRHELPTDD 124

BLAST of HG10020489 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.8e-13
Identity = 37/90 (41.11%), Postives = 48/90 (53.33%), Query Frame = 0

Query: 166 GSCGGKARREVAASPMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWL 225
           G  G +     A S +  +E   V+   G   + C +CK+ M  G    KLPC H +H  
Sbjct: 223 GGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGD 282

Query: 226 CILPWLRKRNTCPCCRFQLPTDDIFGEIQR 256
           CI+PWL  RN+CP CRFQL TDD   E +R
Sbjct: 283 CIVPWLGTRNSCPVCRFQLETDDAEYEEER 312

BLAST of HG10020489 vs. ExPASy Swiss-Prot
Match: Q9VE61 (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN=CG7694 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.8e-13
Identity = 37/81 (45.68%), Postives = 51/81 (62.96%), Query Frame = 0

Query: 178 ASPMAVVELP--SVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRN 237
           AS  A++ELP   +     G  +EC +CKE   EG+    LPC H FH  CIL WL+K N
Sbjct: 46  ASKRAILELPVHEIVKSDEGGDLECSVCKEPAEEGQKYRILPCKHEFHEECILLWLKKTN 105

Query: 238 TCPCCRFQLPTDD-IFGEIQR 256
           +CP CR++L TDD ++ E++R
Sbjct: 106 SCPLCRYELETDDPVYEELRR 126

BLAST of HG10020489 vs. ExPASy TrEMBL
Match: A0A5A7TLA8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002040 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 254/274 (92.70%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAAL+ LSPP L+DLSHSIFS++H HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA
Sbjct: 1   MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT
Sbjct: 61  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRATLCRIYS+S+LTISG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 121 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 180

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           MAVVELPSVAVGGGG  VECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC
Sbjct: 181 MAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+FGEIQRLWEIL KVGSTMCT SDGD
Sbjct: 241 RFQLPTDDVFGEIQRLWEILFKVGSTMCT-SDGD 273

BLAST of HG10020489 vs. ExPASy TrEMBL
Match: A0A1S4DUS9 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103486430 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 254/274 (92.70%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAAL+ LSPP L+DLSHSIFS++H HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA
Sbjct: 11  MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 70

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT
Sbjct: 71  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 130

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRATLCRIYS+S+LTISG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASP
Sbjct: 131 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASP 190

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           MAVVELPSVAVGGGG  VECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 MAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+FGEIQRLWEIL KVGSTMCT SDGD
Sbjct: 251 RFQLPTDDVFGEIQRLWEILFKVGSTMCT-SDGD 283

BLAST of HG10020489 vs. ExPASy TrEMBL
Match: A0A0A0LAQ3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736610 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.3e-143
Identity = 254/273 (93.04%), Postives = 263/273 (96.34%), Query Frame = 0

Query: 2   AALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR 61
           AAL+ LSPP L+DLSHSIFS+IH HRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR
Sbjct: 12  AALATLSPPRLADLSHSIFSEIHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLAR 71

Query: 62  LLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK 121
            LLSALRRLSRPFQSPSKLLPYHPS AAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK
Sbjct: 72  FLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPITK 131

Query: 122 WRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASPM 181
           WRATLC+IYSDS+LT+SG+ATGGGGALIPYI+TV RCWKFVGFVGSCGGK RREVAASPM
Sbjct: 132 WRATLCKIYSDSLLTVSGLATGGGGALIPYIDTVVRCWKFVGFVGSCGGKVRREVAASPM 191

Query: 182 AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR 241
           AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR
Sbjct: 192 AVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCR 251

Query: 242 FQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           FQLPTDD+ GEIQRLWEIL KVGSTMCT SDGD
Sbjct: 252 FQLPTDDVLGEIQRLWEILFKVGSTMCT-SDGD 283

BLAST of HG10020489 vs. ExPASy TrEMBL
Match: A0A6J1CX45 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN=LOC111015097 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.1e-128
Identity = 231/274 (84.31%), Postives = 250/274 (91.24%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAALSALSP  LSDLSHSIFSDIHH RRRLTFILSSPTLFSLTLRHLNSLSLSHKSLL+A
Sbjct: 40  MAALSALSPLHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 99

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRL  PFQ P+K LP HPS AAI+P+DLDAAVLLLLLCEVRQHNPAAL TP+ 
Sbjct: 100 RFLLSALRRLCHPFQPPAKSLPQHPSTAAITPRDLDAAVLLLLLCEVRQHNPAALETPLP 159

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARREVAASP 180
           KWRA LCRIYSDSIL+ISG+ +GGG  ++PY+ETVARCWKFVGFVGSCGGK +REVAASP
Sbjct: 160 KWRAALCRIYSDSILSISGIISGGGAVMVPYVETVARCWKFVGFVGSCGGKGKREVAASP 219

Query: 181 MAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCC 240
           +AVVELPSVA     AAVECVICKEEMGEGRDAC+LPC+HLFHWLCILPWLRKRNTCPCC
Sbjct: 220 VAVVELPSVA---AAAAVECVICKEEMGEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 279

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           RFQLPTDD+F EIQRLWEILLK+GST+ T+SDGD
Sbjct: 280 RFQLPTDDVFAEIQRLWEILLKLGSTI-TTSDGD 309

BLAST of HG10020489 vs. ExPASy TrEMBL
Match: A0A6J1F6V7 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita moschata OX=3662 GN=LOC111442657 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.9e-126
Identity = 234/275 (85.09%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MAALS LS P LSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLL+A
Sbjct: 11  MAALSTLSSPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLIA 70

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120
           R LLSALRRLSRPFQ+PSK  P+HPS AA++P+DLDAAVLLLLLC+VRQHNPAAL TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRATLCRIYSDSILTISGVATGGGGALIPYIETVARCWKFVGFVGS-CGGKARREVAAS 180
           +WR+TLCRIYSDS+LTISG+ATGG GA IPYIETVARCWKFVGFV S  GGKARREVAAS
Sbjct: 131 QWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPC 240
           P+AVVELPSVAV GG  AVECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRKRNTCPC
Sbjct: 191 PVAVVELPSVAV-GGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPC 250

Query: 241 CRFQLPTDDIFGEIQRLWEILLKVGSTMCTSSDGD 275
           CRFQLPTDDIFGEIQRLW+IL+KVGS    SSDGD
Sbjct: 251 CRFQLPTDDIFGEIQRLWDILVKVGS----SSDGD 280

BLAST of HG10020489 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.0e-67
Identity = 148/274 (54.01%), Postives = 176/274 (64.23%), Query Frame = 0

Query: 1   MAALSALSPPCLSDLSHSIFSDIHHHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60
           MA+LSALSP  L++L+HSI S  HHHRRRL  +LSSPTLFSLTLRHL SLSL  K+ L+A
Sbjct: 10  MASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLSLPDKTHLIA 69

Query: 61  RLLLSALRRLSRPFQSPSKLLPYHPSIA-AISPQDLDAAVLLLLLCEVRQHNPAALRTPI 120
             LLS L     P     K   +H S A  +  +DLDA VLLL LCE  Q +P  L    
Sbjct: 70  NHLLSLL----HPLLIHRK---HHSSYAVTMKLRDLDAVVLLLFLCETHQLHPDVLEASA 129

Query: 121 TKWRATLCRIYSDSILT-ISGVATGGGGALIPYIETVARCWKFVGFVGSCGGKARR---- 180
             WR  L   YS+++L+  SG+ T   G L+PYIET+ RC +FV  +G      RR    
Sbjct: 130 DNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGYNHLRRRDQKE 189

Query: 181 --EVAASPMAVVELPSVAV----GGGGAAVECVICKEEMGEGRDACKLPCDHLFHWLCIL 240
             +V A+  AVV L +V V          VECVICKEEM EGRD C++PC H FHW CIL
Sbjct: 190 GYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPCQHFFHWKCIL 249

Query: 241 PWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLK 263
           PWL K+NTCP CRFQLPTDD+F EIQRLWEIL+K
Sbjct: 250 PWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

BLAST of HG10020489 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 3.0e-15
Identity = 29/49 (59.18%), Postives = 38/49 (77.55%), Query Frame = 0

Query: 200 CVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCRFQLPTDD 249
           C +CK+EM  G  A +LPC+H +H  CI+PWL+ RNTCP CR++LPTDD
Sbjct: 309 CAVCKDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDD 357

BLAST of HG10020489 vs. TAIR 10
Match: AT5G64920.1 (COP1-interacting protein 8 )

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-14
Identity = 37/90 (41.11%), Postives = 48/90 (53.33%), Query Frame = 0

Query: 166 GSCGGKARREVAASPMAVVELPSVAVGGGGAAVECVICKEEMGEGRDACKLPCDHLFHWL 225
           G  G +     A S +  +E   V+   G   + C +CK+ M  G    KLPC H +H  
Sbjct: 223 GGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGD 282

Query: 226 CILPWLRKRNTCPCCRFQLPTDDIFGEIQR 256
           CI+PWL  RN+CP CRFQL TDD   E +R
Sbjct: 283 CIVPWLGTRNSCPVCRFQLETDDAEYEEER 312

BLAST of HG10020489 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 4.4e-14
Identity = 30/51 (58.82%), Postives = 38/51 (74.51%), Query Frame = 0

Query: 197 AVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPCCRFQLPTD 248
           AV C +CKEEM  G++  +LPC H +H  CI+PWL  RNTCP CRF+LP+D
Sbjct: 369 AVCCAVCKEEMIVGKEVAELPCRHKYHSECIVPWLGIRNTCPVCRFELPSD 419

BLAST of HG10020489 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.7e-14
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 178 ASPMAVVELPSVAVGGGGAAVE---CVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKR 237
           AS  A+  LP+V V       E   C +C +E  +G D  ++PC H+FH  C+LPWL   
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 238 NTCPCCRFQLPTDD 249
           N+CP CRF+LPTDD
Sbjct: 251 NSCPVCRFELPTDD 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0044054.12.8e-14392.70E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo var. makuwa] >TYK25... [more]
XP_016899723.12.8e-14392.70PREDICTED: E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis melo][more]
XP_004137953.14.7e-14393.04E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucumis sativus] >KGN58913.1 hyp... [more]
XP_038905798.15.2e-14293.80E3 ubiquitin-protein ligase SGR9, amyloplastic [Benincasa hispida][more]
XP_022145706.14.3e-12884.31E3 ubiquitin-protein ligase SGR9, amyloplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q8GXF82.8e-6654.01E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G... [more]
Q9P0P02.5e-1447.89E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1[more]
Q3T0W33.3e-1449.30E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1[more]
Q9SPL22.8e-1341.11E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
Q9VE612.8e-1345.68E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7TLA81.3e-14392.70E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S4DUS91.3e-14392.70E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LAQ32.3e-14393.04RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736610 P... [more]
A0A6J1CX452.1e-12884.31E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN... [more]
A0A6J1F6V71.9e-12685.09E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT5G02750.12.0e-6754.01RING/U-box superfamily protein [more]
AT5G08139.13.0e-1559.18RING/U-box superfamily protein [more]
AT5G64920.12.0e-1441.11COP1-interacting protein 8 [more]
AT5G60820.14.4e-1458.82RING/U-box superfamily protein [more]
AT3G19950.15.7e-1445.95RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 200..240
e-value: 3.5E-5
score: 33.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 199..241
e-value: 2.2E-11
score: 43.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 200..241
score: 11.884405
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 193..251
e-value: 1.3E-17
score: 64.7
NoneNo IPR availablePANTHERPTHR15710:SF67E3 UBIQUITIN-PROTEIN LIGASE SGR9, AMYLOPLASTICcoord: 1..269
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 1..269
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 194..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020489.1HG10020489.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009630 gravitropism
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0009501 amyloplast
molecular_function GO:0061630 ubiquitin protein ligase activity