HG10020385 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020385
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Location	Chr04: 31349050 .. 31349370 (-)
RNA-Seq Expression	HG10020385
Synteny	HG10020385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTACATTGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCCATCGCCCCTCCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAAGTCGAGAACTTGAAAAACTTTCCTTGGATTCCTAA

mRNA sequence

ATGGTCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTACATTGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCCATCGCCCCTCCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAAGTCGAGAACTTGAAAAACTTTCCTTGGATTCCTAA

Coding sequence (CDS)

ATGGTCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTACATTGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCCATCGCCCCTCCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAAGTCGAGAACTTGAAAAACTTTCCTTGGATTCCTAA

Protein sequence

MVNSLVFLVTQEEVIALLFCYIVSLSDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGSRELEKLSLDS

Homology

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BLAST of HG10020385 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 297 EMKKASSQGIRELEKLSLDS 316

[Close]

BLAST of HG10020385 vs. NCBI nr
Match: KAA0044157.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK24978.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 297 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 356

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 357 EMKKASSQGIRELEKLSLDS 376

[Close]

BLAST of HG10020385 vs. NCBI nr
Match: XP_004137730.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_011651872.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_031738324.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >KGN58784.1 hypothetical protein Csa_000714 [Cucumis sativus])

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 297 EMKKASSQGIRELEKLSLDS 316

[Close]

BLAST of HG10020385 vs. NCBI nr
Match: XP_016899730.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 206 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 265

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 266 EMKKASSQGIRELEKLSLDS 285

[Close]

BLAST of HG10020385 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 164.5 bits (415), Expect = 5.1e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 297 EMKKASSQGIRELEKLSLDS 316

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BLAST of HG10020385 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-12
Identity = 30/58 (51.72%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 33  LEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 91
           L+GP ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 225 LDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

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BLAST of HG10020385 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-09
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 0


Query: 20  CYI--VSLSDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQP 79
           C++  + +SD  S W E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ 
Sbjct: 230 CFLNKLCISDKLSHWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQS 289

Query: 80  PIAPPYKRALEMKKA 92
           P  PPYK+ALE+K++
Sbjct: 290 PTNPPYKKALELKRS 303

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BLAST of HG10020385 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.0e-09
Identity = 27/48 (56.25%), Postives = 36/48 (75.00%), Query Frame = 0


Query: 42  PFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMK 90
           PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+K
Sbjct: 234 PFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIELK 279

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BLAST of HG10020385 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 8.3e-06
Identity = 28/60 (46.67%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 43  FLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPPIAPPYKRALEMKKA 92
           F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  P YK AL+ KK+
Sbjct: 231 FVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTDPAYKAALDKKKS 290

[Close]

BLAST of HG10020385 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.5e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 297 EMKKASSQGIRELEKLSLDS 316

[Close]

BLAST of HG10020385 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.5e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 297 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 356

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 357 EMKKASSQGIRELEKLSLDS 376

[Close]

BLAST of HG10020385 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.5e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 297 EMKKASSQGIRELEKLSLDS 316

[Close]

BLAST of HG10020385 vs. ExPASy TrEMBL
Match: A0A1S4DVJ2 (putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.5e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 206 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 265

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLSLDS
Sbjct: 266 EMKKASSQGIRELEKLSLDS 285

[Close]

BLAST of HG10020385 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.2e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 86
           DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRAL 296

Query: 87  EMKKASSQGSRELEKLSLDS 107
           EMKKASSQG RELEKLS DS
Sbjct: 297 EMKKASSQGIRELEKLSSDS 316

[Close]

BLAST of HG10020385 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 2.6e-31
Identity = 60/69 (86.96%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 27  DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRA 86
           DCSSGWLEGPNKVYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRA
Sbjct: 237 DCSSGWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRA 296

Query: 87  LEMKKASSQ 95
           LE+KKAS+Q
Sbjct: 297 LELKKASAQ 305

The following BLAST results are available for this feature:

BLAST of HG10020385 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442455.1	1.3e-37	98.75	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]
KAA0044157.1	1.3e-37	98.75	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cu...	[more]
XP_004137730.1	1.3e-37	98.75	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativ...	[more]
XP_016899730.1	1.3e-37	98.75	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]
XP_038903231.1	5.1e-37	97.50	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]

BLAST of HG10020385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	1.2e-12	51.72	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
P38238	1.2e-09	45.33	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]
O36015	8.0e-09	56.25	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q22031	8.3e-06	46.67	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]

BLAST of HG10020385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6C8	6.5e-38	98.75	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A5A7TQN9	6.5e-38	98.75	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A0A0LCV6	6.5e-38	98.75	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]
A0A1S4DVJ2	6.5e-38	98.75	putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 OS=...	[more]
A0A6J1IYY9	3.2e-37	97.50	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]

BLAST of HG10020385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	2.6e-31	86.96	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 25..97
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 25..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10020385.1	HG10020385.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0006508	proteolysis
biological_process	GO:0002128	tRNA nucleoside ribose methylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005838	proteasome regulatory particle
molecular_function	GO:0070122	isopeptidase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0008175	tRNA methyltransferase activity