HG10020308 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10020308 vs. NCBI nr
Match: XP_038904628.1 (putative E3 ubiquitin-protein ligase XBAT31 isoform X1 [Benincasa hispida])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 415/436 (95.18%), Postives = 425/436 (97.48%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSCADRHE+GFFFRALQNGDVEVVRAMVETD SILHHTTNHQR+TPLHVAAANGQI
Sbjct: 1   MGQGLSCADRHETGFFFRALQNGDVEVVRAMVETDSSILHHTTNHQRSTPLHVAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLD  VNPDILNRHNQTPLM+AAMSGRI CVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDILNRHNQTPLMLAAMSGRITCVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGHSDCLQAILSAA+TTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQ ECVRKLLN
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQTECVRKLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQC 
Sbjct: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           LQADVDIDAYV+NDLASEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LQADVDIDAYVDNDLASEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQEISPLKLKGLRTSNFGEENNSLKSLSA 420
           TTA PTAPVCPFCRSSITQLLVAEV+SNDNVDQE SPLKLKGLRTSNF EEN+SLKSLSA
Sbjct: 361 TTACPTAPVCPFCRSSITQLLVAEVRSNDNVDQENSPLKLKGLRTSNFNEENSSLKSLSA 420

Query: 421 LFGKFSEQNAGTCVKP 437
           LFGKFSEQNAGTC+KP
Sbjct: 421 LFGKFSEQNAGTCIKP 436

BLAST of HG10020308 vs. NCBI nr
Match: KAA0044085.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucumis melo var. makuwa] >TYK25052.1 putative E3 ubiquitin-protein ligase XBAT31 [Cucumis melo var. makuwa])

HSP 1 Score: 830.1 bits (2143), Expect = 8.9e-237
Identity = 400/437 (91.53%), Postives = 421/437 (96.34%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC  RHE+GFFFRAL NGDV+V+RAMV+TDPSILHHT NHQR+TPLH AAANGQI
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILS+LLDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 61  DILSILLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AA+YGHSDCLQAILSAA+TTPVSDSWGFARFVN+RDGDGSTPLHLASRQSQPECVR LLN
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDL SEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKF EQNAGTC+KP
Sbjct: 421 ALFGKFGEQNAGTCIKP 437

BLAST of HG10020308 vs. NCBI nr
Match: XP_008442556.1 (PREDICTED: putative E3 ubiquitin-protein ligase XBAT31 [Cucumis melo])

HSP 1 Score: 827.4 bits (2136), Expect = 5.8e-236
Identity = 399/437 (91.30%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC  RHE+GFFFRAL NGDV+V+RAMV+TDPSILHHT NHQR+TPLH AAANGQI
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILS+ LDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 61  DILSIPLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AA+YGHSDCLQAILSAA+TTPVSDSWGFARFVN+RDGDGSTPLHLASRQSQPECVR LLN
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDL SEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKF EQNAGTC+KP
Sbjct: 421 ALFGKFGEQNAGTCIKP 437

BLAST of HG10020308 vs. NCBI nr
Match: XP_011651899.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucumis sativus] >KAE8651011.1 hypothetical protein Csa_002343 [Cucumis sativus])

HSP 1 Score: 817.4 bits (2110), Expect = 6.0e-233
Identity = 395/437 (90.39%), Postives = 418/437 (95.65%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC   HE+G FFRAL NGDV+VVRAMV+ DPS+L+HT NHQR+TPLH AAANG I
Sbjct: 1   MGQGLSCTHSHETGLFFRALHNGDVDVVRAMVDNDPSVLYHTINHQRSTPLHAAAANGHI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 61  DILSMLLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           A++YGHSDCLQAILSAA++TPVSDSWGFARFVN+RDGDGSTPLHLASRQSQ ECVR LLN
Sbjct: 121 ASFYGHSDCLQAILSAAHSTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQLECVRMLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDLASEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLASEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKFSEQNAGTC+KP
Sbjct: 421 ALFGKFSEQNAGTCIKP 437

BLAST of HG10020308 vs. NCBI nr
Match: XP_022971571.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita maxima])

HSP 1 Score: 783.5 bits (2022), Expect = 9.5e-223
Identity = 379/435 (87.13%), Postives = 404/435 (92.87%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSCADRH +G FFRALQNGDVEVVRA++E D SILH TT HQ  + LHVAAANGQ 
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLD  VNPD+LNRHNQTPLM+AAMSG+IACVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGHSDCLQAILSAA+TTPVSDSWGFARFVNIRDGDGSTPLHL++RQSQPECVR LL+
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALVCVSTCSC GSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
            CAALLNPSSPEPLVWPSPLKLII+L PDAK+LLEKALMDANMEREKAI KETF+TP C 
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
            Q+D+DID+ ++NDLASEA D++LC ICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQICNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQEISPLKLKGLRTSNFGEENNSLKSLSA 420
           TTA PTAPVCPFCR SITQLLVAEVKS+D VDQEISPLKLKGL TSNF EEN+SLKSLS 
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 420

Query: 421 LFGKFSEQNAGTCVK 436
           LFGK SEQN+GTCVK
Sbjct: 421 LFGKLSEQNSGTCVK 435

BLAST of HG10020308 vs. ExPASy Swiss-Prot
Match: Q94B55 (Putative E3 ubiquitin-protein ligase XBAT31 OS=Arabidopsis thaliana OX=3702 GN=XBAT31 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.1e-144
Identity = 262/450 (58.22%), Postives = 334/450 (74.22%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQ +SC  R E G  F ++Q GD+  +R ++ T+PS+L+ TT + R + LHVAAANGQI
Sbjct: 1   MGQSMSCGSRPEHG-IFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +ILS+LL+R  NPD+LNRH QTPLM+AAM GRI+CV++L E GANILMFDS++RRTCLHY
Sbjct: 61  EILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHLA+RQ +PECV  LL+
Sbjct: 121 AAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLD 180

Query: 181 NGALVCVSTC--SCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 240
           +G+LVC ST      GS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KHK
Sbjct: 181 SGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHK 240

Query: 241 HQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQ 300
           H  C ALLNPSS EPLVWPSPLK I +L+ +AK+LLE+ALM+AN EREK ILK T  +  
Sbjct: 241 HGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLP 300

Query: 301 CHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKP 360
               +D D       D  SE  D++LCCICFEQVC IE + CGH+MCA CTL+LCCH KP
Sbjct: 301 SPSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKP 360

Query: 361 NPTTASPTAPVCPFCRSSITQLLVAEVKSNDN-----------VDQE---ISPLKL-KGL 420
           NPTT++ T PVCPFCRS+I  L+VA+  +N+N           VD+E   +S  K  K  
Sbjct: 361 NPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHR 420

Query: 421 RTSNFGEENNSLKSLSAL--FGKFSEQNAG 432
           R+ N GEE++S   LS +  FG+ + + +G
Sbjct: 421 RSINLGEESSSFMGLSTIGSFGRITGRGSG 442

BLAST of HG10020308 vs. ExPASy Swiss-Prot
Match: Q65XV2 (E3 ubiquitin-protein ligase XB3 OS=Oryza sativa subsp. japonica OX=39947 GN=XB3 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 4.7e-140
Identity = 246/436 (56.42%), Postives = 316/436 (72.48%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MG G+SCA   +   FFRA Q GD++ + A++  DPS+    T + R + LH+AAANG+I
Sbjct: 1   MGHGVSCARTGDEHDFFRAAQLGDLDALAALLAADPSLARRATLYDRLSVLHIAAANGRI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           ++LSM LDR   PD +NRH QTPLM+AAM G+I CV +L++A ANILMFDS+H RTCLH+
Sbjct: 61  EVLSMFLDRGAPPDAVNRHKQTPLMLAAMHGKIDCVLKLLQADANILMFDSVHARTCLHH 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGH DCLQAIL+AA TTPV+DSWGFARFVN+RD  G+TPLHLA+RQ +P CV+ LL 
Sbjct: 121 AAYYGHVDCLQAILAAAQTTPVADSWGFARFVNVRDDHGATPLHLAARQGRPGCVQVLLE 180

Query: 181 NGALVCVSTCSCA--GSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 240
           NGA+V   T S    GS+ LHLAAR G+L+C+R+LLAWGADR Q DS GRIP++VALK  
Sbjct: 181 NGAIVSALTGSYGFPGSTSLHLAARSGNLDCIRKLLAWGADRLQRDSAGRIPYSVALKRN 240

Query: 241 HQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQ 300
           H  CAALLNP+S EP+VWPSPLK I +L+P+AK LLE ALM+AN EREK IL  T    +
Sbjct: 241 HGACAALLNPTSAEPMVWPSPLKFISELEPEAKALLEAALMEANREREKKILNGT----K 300

Query: 301 CHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKP 360
             L +    D   ++D  SE  D++LCCICF+Q C IE Q CGH+MCA CTL+LCCH KP
Sbjct: 301 YSLPSPSPGDDSADDDACSEVSDTELCCICFDQACTIEVQDCGHQMCAPCTLALCCHNKP 360

Query: 361 NPTTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQ--EISPLKLKGLRTSNFGEENNSLK 420
           NPTT +P +P CPFCR SI++L+VA+ +S  + D+   +   + +  R+ N  E ++S K
Sbjct: 361 NPTTLTPPSPACPFCRGSISRLVVAQTRSACDPDKPSSLQLTRKRSRRSHNLSEGSSSFK 420

Query: 421 SLSALFGKFSEQNAGT 433
            L +  G FS+   G+
Sbjct: 421 GLPSAMGSFSKLGRGS 432

BLAST of HG10020308 vs. ExPASy Swiss-Prot
Match: Q94CT7 (Probable E3 ubiquitin-protein ligase XBOS31 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS31 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 6.9e-115
Identity = 221/437 (50.57%), Postives = 287/437 (65.68%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MG GLSC+   +    FRA Q GD+  + A++  DP++    T + R T LH+AAANG++
Sbjct: 1   MGHGLSCSRDTDEYDLFRAAQLGDIHALSALLAADPALARRATVYDRFTALHIAAANGRL 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
            +LSMLLDR  + D+L+R  QTPLM+AAM G   CV RL+  GAN+L FDS   RTCLH+
Sbjct: 61  QVLSMLLDRDGDVDVLSRKKQTPLMVAAMRGNTECVVRLLRGGANVLTFDSPRARTCLHH 120

Query: 121 AAYYGHSDCLQAILSAANTT--PVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKL 180
           AAYYGH++CLQAIL AA     PV+ SWGFARFVN+RD  G+TPLHLA+R ++  CVR L
Sbjct: 121 AAYYGHAECLQAILGAAAQAQGPVAASWGFARFVNVRDERGATPLHLAARHARASCVRLL 180

Query: 181 LNNGALVCVSTC--SCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALK 240
           L+ GA+V   T      GS+ LHLAAR GS+EC+RELLAWGADR Q DS GRI + VA++
Sbjct: 181 LDKGAIVSAPTAVYGFPGSTALHLAARAGSMECIRELLAWGADRLQRDSAGRIAYAVAMR 240

Query: 241 HKHQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFIT 300
             H+ CAALLNP++ EP+VWPSPLK I +L+ DAK LLE ALM+AN EREK IL      
Sbjct: 241 RGHRACAALLNPAAAEPIVWPSPLKFIGELEADAKALLEAALMEANREREKRILH----- 300

Query: 301 PQCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHK 360
                 +D++I    E + + +  + + C ICFEQ C++E + CGH+MCA CTL++CCH 
Sbjct: 301 -----GSDINIKGGDEEEESED--EEEACNICFEQACSMEVKECGHQMCAACTLAICCHS 360

Query: 361 KPNPTTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQEISPLKLKGLRTSNFGEENNSLK 420
           KPNP T     P CPFCR++I++L+VA   SN          K    R S     ++S K
Sbjct: 361 KPNPKTLLLHPPACPFCRTTISRLVVATTNSN----------KTNSRRRSR--SRSSSFK 413

Query: 421 -SLSALFGKFSEQNAGT 433
             LS+  G FS    G+
Sbjct: 421 GGLSSAMGSFSRIGRGS 413

BLAST of HG10020308 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.4e-35
Identity = 113/402 (28.11%), Postives = 174/402 (43.28%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MG    C+   E      A ++GD    + +++ +P +  ++T     +PLH AAA G  
Sbjct: 1   MGNSFGCSASGER--LVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHN 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +I+ +LL+   + +  N   QT LM A   G    VQ L+    N+   D +  RT LH+
Sbjct: 61  EIVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHF 120

Query: 121 AAYYGHSDCLQAILS------AANTTP---------VSDSWGFARFVNIRDGDGSTPLHL 180
           AA  GH+ C++ +L+        N+ P          S+    ++FVN     G T LH+
Sbjct: 121 AAVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHM 180

Query: 181 ASRQSQPECVRKLLNNGALVCVSTCSC--------AGSSPLHLAARGGSLECVRELLAWG 240
           A+     +CV+ LL+  A V   T           AGS+PLH AA GG+L+C + LLA G
Sbjct: 181 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 240

Query: 241 ADRFQFDSYGRIPFTVALKHKHQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKA 300
           A +   +  G +P  +A          LL+P+S           ++I   P +  L    
Sbjct: 241 ARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNS----------DVVIPAFPHSNYLSLPL 300

Query: 301 LMDANMEREKAILKETFITPQCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEA 360
           L   N+ RE  +   T                           + D+C +C E+ C + A
Sbjct: 301 LSILNIAREFGLQSATI------------------------GDEVDICAVCLERTCTVAA 360

Query: 361 QPCGHRMCAHCTLSLCCHKK-PNPTTASPTAPVCPFCRSSIT 379
           + C H++C  C L LC     P+ T   P +  CP CR  IT
Sbjct: 361 EGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIPCPLCRHGIT 366

BLAST of HG10020308 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.4e-35
Identity = 113/408 (27.70%), Postives = 179/408 (43.87%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           +G    C+   E      A ++GD++   A++E +P +  ++T   R +PLH +AA G  
Sbjct: 7   VGDSFGCSATGER--LVSAARDGDIQEAMALLELNPRLARYSTFGIRNSPLHYSAAKGHH 66

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +I+S+L++  V+ ++ N   QT LM A + G    VQ L+   ANI   D     T +H+
Sbjct: 67  EIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGATAIHF 126

Query: 121 AAYYGHSDCLQ--------------AILSAANTTPVS----DSWGFARFVNIRDGDGSTP 180
           AA  GH+ CL+              +++ A  T   +    D+    R +N +   G TP
Sbjct: 127 AALKGHTRCLRLLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDGGVTP 186

Query: 181 LHLASRQSQPECVRKLLNNGALVCVSTCS--------CAGSSPLHLAARGGSLECVRELL 240
           LHLA+     ECV+ LL+ GA V   T +         +GS+PLH AA GGS  C + L+
Sbjct: 187 LHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCCQLLV 246

Query: 241 AWGADRFQFDSYGRIPFTVALKHKHQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILL 300
           A GA+    ++ G  P  VA      +   +L   S  P          +++ P + + L
Sbjct: 247 AAGANMRAQNTNGLTPLMVARSWHKSSVEGILTKRSEVP----------VRILPSSYLSL 306

Query: 301 EKALMDANMEREKAILKETFITPQCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCN 360
              LM       +   ++T ++  CH                      D C IC +  C 
Sbjct: 307 --PLMSIVKIARECGWRKTSVSSVCH----------------------DPCAICLDTECT 366

Query: 361 IEAQPCGHRMCAHCTLSLCCHKKPNPTTAS-PTAPVCPFCRSSITQLL 382
           + A+ CGH  C  C L LC     + +    P +  CP CR +I   +
Sbjct: 367 VSAEGCGHEFCTKCALYLCATASSSTSIRGVPGSIPCPLCRHTIVSFV 378

BLAST of HG10020308 vs. ExPASy TrEMBL
Match: A0A5A7TRQ8 (Putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001730 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.3e-237
Identity = 400/437 (91.53%), Postives = 421/437 (96.34%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC  RHE+GFFFRAL NGDV+V+RAMV+TDPSILHHT NHQR+TPLH AAANGQI
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILS+LLDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 61  DILSILLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AA+YGHSDCLQAILSAA+TTPVSDSWGFARFVN+RDGDGSTPLHLASRQSQPECVR LLN
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDL SEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKF EQNAGTC+KP
Sbjct: 421 ALFGKFGEQNAGTCIKP 437

BLAST of HG10020308 vs. ExPASy TrEMBL
Match: A0A1S3B5H1 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo OX=3656 GN=LOC103486391 PE=4 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 2.8e-236
Identity = 399/437 (91.30%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC  RHE+GFFFRAL NGDV+V+RAMV+TDPSILHHT NHQR+TPLH AAANGQI
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILS+ LDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 61  DILSIPLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AA+YGHSDCLQAILSAA+TTPVSDSWGFARFVN+RDGDGSTPLHLASRQSQPECVR LLN
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDL SEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKF EQNAGTC+KP
Sbjct: 421 ALFGKFGEQNAGTCIKP 437

BLAST of HG10020308 vs. ExPASy TrEMBL
Match: A0A0A0LFK7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734290 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.9e-233
Identity = 395/437 (90.39%), Postives = 418/437 (95.65%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSC   HE+G FFRAL NGDV+VVRAMV+ DPS+L+HT NHQR+TPLH AAANG I
Sbjct: 81  MGQGLSCTHSHETGLFFRALHNGDVDVVRAMVDNDPSVLYHTINHQRSTPLHAAAANGHI 140

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLDR VNPDILNRHNQTPLM+AAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY
Sbjct: 141 DILSMLLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 200

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           A++YGHSDCLQAILSAA++TPVSDSWGFARFVN+RDGDGSTPLHLASRQSQ ECVR LLN
Sbjct: 201 ASFYGHSDCLQAILSAAHSTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQLECVRMLLN 260

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALV VSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 261 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 320

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
           TCAALLNPSSPEPL+WPSPLKLIIKLDPDAK+LLEKALMDANMEREKAILKETFIT QC 
Sbjct: 321 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 380

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
           L+ADVDIDAYV+NDLASEA D+DLCCICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 381 LEADVDIDAYVDNDLASEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 440

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVK-SNDNVDQEISPLKLKGLRTSNFGEENNSLKSLS 420
           TTA PTAPVCPFCRSSI++LLVAEVK +ND+VDQEISPLKLKGLRTSNF EENNSLKSLS
Sbjct: 441 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 500

Query: 421 ALFGKFSEQNAGTCVKP 437
           ALFGKFSEQNAGTC+KP
Sbjct: 501 ALFGKFSEQNAGTCIKP 517

BLAST of HG10020308 vs. ExPASy TrEMBL
Match: A0A6J1I3N0 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita maxima OX=3661 GN=LOC111470249 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.6e-223
Identity = 379/435 (87.13%), Postives = 404/435 (92.87%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSCADRH +G FFRALQNGDVEVVRA++E D SILH TT HQ  + LHVAAANGQ 
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLD  VNPD+LNRHNQTPLM+AAMSG+IACVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGHSDCLQAILSAA+TTPVSDSWGFARFVNIRDGDGSTPLHL++RQSQPECVR LL+
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALVCVSTCSC GSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
            CAALLNPSSPEPLVWPSPLKLII+L PDAK+LLEKALMDANMEREKAI KETF+TP C 
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
            Q+D+DID+ ++NDLASEA D++LC ICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQICNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQEISPLKLKGLRTSNFGEENNSLKSLSA 420
           TTA PTAPVCPFCR SITQLLVAEVKS+D VDQEISPLKLKGL TSNF EEN+SLKSLS 
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 420

Query: 421 LFGKFSEQNAGTCVK 436
           LFGK SEQN+GTCVK
Sbjct: 421 LFGKLSEQNSGTCVK 435

BLAST of HG10020308 vs. ExPASy TrEMBL
Match: A0A6J1GBC7 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita moschata OX=3662 GN=LOC111452429 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.3e-222
Identity = 378/435 (86.90%), Postives = 403/435 (92.64%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQGLSCADRH +G FFRALQNGDVEVVRA++E D SILH TT HQ  + LHVAAANGQ 
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           DILSMLLD  VNPD+LNRHNQTPLM+AAMSG+IACVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGHSDCLQAILSAA+TTPVSDSWGFARFVNIRDGDGSTPLHL++RQSQPECVR LL+
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 181 NGALVCVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240
           NGALVCVSTCSC GSSPLHLAARGGSLECVRELLAWGADRFQ+DSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQYDSYGRIPFTVALKHKHQ 240

Query: 241 TCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQCH 300
            CAALLNPSSPEPLVWPSPLKLII+L PDAK+LLEKALMDANMEREKAI KETF+TP C 
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 301 LQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360
            Q+D+DID+ ++NDLASEA D++LC ICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 361 TTASPTAPVCPFCRSSITQLLVAEVKSNDNVDQEISPLKLKGLRTSNFGEENNSLKSLSA 420
           TTA PTAPVCPFCR SITQLLVAEVKS+D VDQEISPLKLKGL TSNF EEN SLKSLS 
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENTSLKSLSI 420

Query: 421 LFGKFSEQNAGTCVK 436
           LFGK SEQN+GTCVK
Sbjct: 421 LFGKLSEQNSGTCVK 435

BLAST of HG10020308 vs. TAIR 10
Match: AT2G28840.1 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 512.7 bits (1319), Expect = 2.9e-145
Identity = 262/450 (58.22%), Postives = 334/450 (74.22%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MGQ +SC  R E G  F ++Q GD+  +R ++ T+PS+L+ TT + R + LHVAAANGQI
Sbjct: 1   MGQSMSCGSRPEHG-IFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQI 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +ILS+LL+R  NPD+LNRH QTPLM+AAM GRI+CV++L E GANILMFDS++RRTCLHY
Sbjct: 61  EILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHY 120

Query: 121 AAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLN 180
           AAYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHLA+RQ +PECV  LL+
Sbjct: 121 AAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLD 180

Query: 181 NGALVCVSTC--SCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 240
           +G+LVC ST      GS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KHK
Sbjct: 181 SGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHK 240

Query: 241 HQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITPQ 300
           H  C ALLNPSS EPLVWPSPLK I +L+ +AK+LLE+ALM+AN EREK ILK T  +  
Sbjct: 241 HGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLP 300

Query: 301 CHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKKP 360
               +D D       D  SE  D++LCCICFEQVC IE + CGH+MCA CTL+LCCH KP
Sbjct: 301 SPSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKP 360

Query: 361 NPTTASPTAPVCPFCRSSITQLLVAEVKSNDN-----------VDQE---ISPLKL-KGL 420
           NPTT++ T PVCPFCRS+I  L+VA+  +N+N           VD+E   +S  K  K  
Sbjct: 361 NPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHR 420

Query: 421 RTSNFGEENNSLKSLSAL--FGKFSEQNAG 432
           R+ N GEE++S   LS +  FG+ + + +G
Sbjct: 421 RSINLGEESSSFMGLSTIGSFGRITGRGSG 442

BLAST of HG10020308 vs. TAIR 10
Match: AT2G28840.2 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 454.5 bits (1168), Expect = 9.5e-128
Identity = 232/391 (59.34%), Postives = 292/391 (74.68%), Query Frame = 0

Query: 60  IDILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLH 119
           + ILS+LL+R  NPD+LNRH QTPLM+AAM GRI+CV++L E GANILMFDS++RRTCLH
Sbjct: 45  LQILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLH 104

Query: 120 YAAYYGHSDCLQAILSAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLL 179
           YAAYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHLA+RQ +PECV  LL
Sbjct: 105 YAAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLL 164

Query: 180 NNGALVCVSTC--SCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKH 239
           ++G+LVC ST      GS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KH
Sbjct: 165 DSGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKH 224

Query: 240 KHQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKALMDANMEREKAILKETFITP 299
           KH  C ALLNPSS EPLVWPSPLK I +L+ +AK+LLE+ALM+AN EREK ILK T  + 
Sbjct: 225 KHGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSL 284

Query: 300 QCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEAQPCGHRMCAHCTLSLCCHKK 359
                +D D       D  SE  D++LCCICFEQVC IE + CGH+MCA CTL+LCCH K
Sbjct: 285 PSPSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNK 344

Query: 360 PNPTTASPTAPVCPFCRSSITQLLVAEVKSNDN-----------VDQE---ISPLKL-KG 419
           PNPTT++ T PVCPFCRS+I  L+VA+  +N+N           VD+E   +S  K  K 
Sbjct: 345 PNPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKH 404

Query: 420 LRTSNFGEENNSLKSLSAL--FGKFSEQNAG 432
            R+ N GEE++S   LS +  FG+ + + +G
Sbjct: 405 RRSINLGEESSSFMGLSTIGSFGRITGRGSG 428

BLAST of HG10020308 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 152.1 bits (383), Expect = 1.0e-36
Identity = 113/402 (28.11%), Postives = 174/402 (43.28%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           MG    C+   E      A ++GD    + +++ +P +  ++T     +PLH AAA G  
Sbjct: 1   MGNSFGCSASGER--LVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHN 60

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +I+ +LL+   + +  N   QT LM A   G    VQ L+    N+   D +  RT LH+
Sbjct: 61  EIVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHF 120

Query: 121 AAYYGHSDCLQAILS------AANTTP---------VSDSWGFARFVNIRDGDGSTPLHL 180
           AA  GH+ C++ +L+        N+ P          S+    ++FVN     G T LH+
Sbjct: 121 AAVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHM 180

Query: 181 ASRQSQPECVRKLLNNGALVCVSTCSC--------AGSSPLHLAARGGSLECVRELLAWG 240
           A+     +CV+ LL+  A V   T           AGS+PLH AA GG+L+C + LLA G
Sbjct: 181 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 240

Query: 241 ADRFQFDSYGRIPFTVALKHKHQTCAALLNPSSPEPLVWPSPLKLIIKLDPDAKILLEKA 300
           A +   +  G +P  +A          LL+P+S           ++I   P +  L    
Sbjct: 241 ARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNS----------DVVIPAFPHSNYLSLPL 300

Query: 301 LMDANMEREKAILKETFITPQCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVCNIEA 360
           L   N+ RE  +   T                           + D+C +C E+ C + A
Sbjct: 301 LSILNIAREFGLQSATI------------------------GDEVDICAVCLERTCTVAA 360

Query: 361 QPCGHRMCAHCTLSLCCHKK-PNPTTASPTAPVCPFCRSSIT 379
           + C H++C  C L LC     P+ T   P +  CP CR  IT
Sbjct: 361 EGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIPCPLCRHGIT 366

BLAST of HG10020308 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 136.7 bits (343), Expect = 4.4e-32
Identity = 112/406 (27.59%), Postives = 175/406 (43.10%), Query Frame = 0

Query: 1   MGQGLSCADRHESGFFFRALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQI 60
           +G    C+   E      A ++GD++  +A+++ +P +  ++T   R +PLH +AA G  
Sbjct: 7   VGNSFGCSASGER--LVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAAQGHH 66

Query: 61  DILSMLLDRKVNPDILNRHNQTPLMMAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120
           +I+S+L++  V+ ++ N   QT LM A   G    V  LI  GANI   D ++  T LH 
Sbjct: 67  EIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGTALHL 126

Query: 121 AAYYGHSDCLQAILS-------------AANTTPVS--DSWGFARFVNIRDGDGSTPLHL 180
           AA  GH  C++ +LS                 T V+  DS      +N     G TPLH+
Sbjct: 127 AALNGHPRCIRILLSEYIPSVPNCWSLLKNKKTSVAGFDSSVLHEVINRAADGGITPLHV 186

Query: 181 ASRQSQPECVRKLLNNGALVCVSTCS--------CAGSSPLHLAARGGSLECVRELLAWG 240
           A+     E V+ LL+ GA V   T           AGS+ LH A+ GG+ +C + L++ G
Sbjct: 187 AALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISKG 246

Query: 241 ADRFQFDSYGRIPFTVALKHKHQTCAALLNPSSPEPLVW----PSPLKLIIKLDPDAKIL 300
           A     +S G  P  VA          +LNP++ +P +     PSP   +          
Sbjct: 247 ACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCL---------- 306

Query: 301 LEKALMDANMEREKAILKETFITPQCHLQADVDIDAYVENDLASEAGDSDLCCICFEQVC 360
                          ++    I  +C          + END  +   D   C +C E+ C
Sbjct: 307 --------------PLMSIVNIAQEC---------GWRENDCLTPCRDP--CAVCLERKC 366

Query: 361 NIEAQPCGHRMCAHCTLSLCCHKKPNPTTA--SPTAPVCPFCRSSI 378
            + A  C H  C +C L L      +  T+  +P +  CP CR+ I
Sbjct: 367 TVAADGCAHEFCTNCALYLSTTSITSSKTSNVTPGSVPCPLCRNGI 375

BLAST of HG10020308 vs. TAIR 10
Match: AT5G60070.1 (ankyrin repeat family protein )

HSP 1 Score: 79.7 bits (195), Expect = 6.3e-15
Identity = 63/235 (26.81%), Postives = 108/235 (45.96%), Query Frame = 0

Query: 19  ALQNGDVEVVRAMVETDPSILHHTTNHQRTTPLHVAAANGQIDILSMLLDRKVNPDI--- 78
           A + GD +VV  +++        T       P H+AA  G++D+L +L++   +P++   
Sbjct: 80  AAEYGDADVVAELIKYYDLEDAETKARNGFDPFHIAAKQGELDVLRVLMEE--HPELSMT 139

Query: 79  LNRHNQTPLMMAAMSGRIACVQRLIE-AGANILMFDSIHRRTCLHYAAYYGHSDCLQAIL 138
           ++  N T L  AA  G +  V+ L+E AG+++      + +T LH AA  GH++ ++AI+
Sbjct: 140 VDLSNTTALHTAAAQGHVEVVEYLLEAAGSSLAAIAKSNGKTALHSAARNGHAEVVKAIV 199

Query: 139 SAANTTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRKLLNNGALVCVSTCSCAG 198
           +    T               D  G TPLH+A +    + V +L+  G    ++     G
Sbjct: 200 AVEPDTATR-----------TDKKGQTPLHMAVKGQSIDVVVELM-KGHRSSLNMADSKG 259

Query: 199 SSPLHLAARGGSLECVRELL---AWGADRFQFDSYGRIPFTVALKHKHQTCAALL 247
           ++ LH+A R G ++ V  LL            +  G  P   A K  H   AA+L
Sbjct: 260 NTALHVATRKGRIKIVELLLDNNETSPSTKAINRAGETPLDTAEKTGHPQIAAVL 300