HG10019837 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019837
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr04: 25998878 .. 25999426 (+)
RNA-Seq Expression	HG10019837
Synteny	HG10019837

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATATCTAAAGAGCTCAGAAACACTTATCAAACGAGTATTAAAGATTCTAATGAACAATCTAAATGCCAATGAACTCGACAAAACAAATCGATCTCGTACAATCCAATTCAACTACTATCCCATTTGTCCAACCCCCGATCTTAACGTTGGAGTCGGCCGCCACTCTGACATTTCGCTGGTGACGGTTCTGTTGCAAGACGATGTCGGCGGGCTCCACATGCGGCGGGGAATCGACGATGAATGGGTTCACGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCAATGGAGATAATGAGCAATGGAGTTTACAAGAGTGTGGAGCATTGTGTGGTGGCCAATGGAAACAGTGATAGAATGTCGATTCCCATATTTATAAATCCAAAACATTCCGCCATTGTTAGGCCACTTCCAGAGGTGCTTGCGACAGGGAAGAAAGCACAGTATAAGGAAGTTGTGTTTTCAGATTATGCTACACATTTCTTTAGTAAGCCACATGATGGGAAGAACACTATTGAGTTTGCCAAAATATGA

mRNA sequence

ATGAAATATCTAAAGAGCTCAGAAACACTTATCAAACGAGTATTAAAGATTCTAATGAACAATCTAAATGCCAATGAACTCGACAAAACAAATCGATCTCGTACAATCCAATTCAACTACTATCCCATTTGTCCAACCCCCGATCTTAACGTTGGAGTCGGCCGCCACTCTGACATTTCGCTGGTGACGGTTCTGTTGCAAGACGATGTCGGCGGGCTCCACATGCGGCGGGGAATCGACGATGAATGGGTTCACGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCAATGGAGATAATGAGCAATGGAGTTTACAAGAGTGTGGAGCATTGTGTGGTGGCCAATGGAAACAGTGATAGAATGTCGATTCCCATATTTATAAATCCAAAACATTCCGCCATTGTTAGGCCACTTCCAGAGGTGCTTGCGACAGGGAAGAAAGCACAGTATAAGGAAGTTGTGTTTTCAGATTATGCTACACATTTCTTTAGTAAGCCACATGATGGGAAGAACACTATTGAGTTTGCCAAAATATGA

Coding sequence (CDS)

ATGAAATATCTAAAGAGCTCAGAAACACTTATCAAACGAGTATTAAAGATTCTAATGAACAATCTAAATGCCAATGAACTCGACAAAACAAATCGATCTCGTACAATCCAATTCAACTACTATCCCATTTGTCCAACCCCCGATCTTAACGTTGGAGTCGGCCGCCACTCTGACATTTCGCTGGTGACGGTTCTGTTGCAAGACGATGTCGGCGGGCTCCACATGCGGCGGGGAATCGACGATGAATGGGTTCACGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCAATGGAGATAATGAGCAATGGAGTTTACAAGAGTGTGGAGCATTGTGTGGTGGCCAATGGAAACAGTGATAGAATGTCGATTCCCATATTTATAAATCCAAAACATTCCGCCATTGTTAGGCCACTTCCAGAGGTGCTTGCGACAGGGAAGAAAGCACAGTATAAGGAAGTTGTGTTTTCAGATTATGCTACACATTTCTTTAGTAAGCCACATGATGGGAAGAACACTATTGAGTTTGCCAAAATATGA

Protein sequence

MKYLKSSETLIKRVLKILMNNLNANELDKTNRSRTIQFNYYPICPTPDLNVGVGRHSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHCVVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKNTIEFAKI

Homology

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BLAST of HG10019837 vs. NCBI nr
Match: XP_011652189.1 (feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical protein Csa_001767 [Cucumis sativus])

HSP 1 Score: 283.5 bits (724), Expect = 1.3e-72
Identity = 140/185 (75.68%), Postives = 162/185 (87.57%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNRSRTIQFNYYPICPTPDLNVGVGRHSDIS 60
           ++YLKSS TLIKRVLKILMN LNANELD+TN++R IQFNYYPI PTPDL+VGV  HSD+S
Sbjct: 180 IEYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVS 239

Query: 61  LVTVLLQDDVGGLHMRRGID---DEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHCVV 120
           L+TVLLQDDVGGLH+RR ID   DEWV VKPVAESLVINIGD +EIMSNG+YKSVEHCVV
Sbjct: 240 LLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVV 299

Query: 121 ANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKNTI 180
           +NGN DRMSIPIF+  ++S  + PLPEVLATGKKA+YKEVVFSDY  +F +KP DGK+T+
Sbjct: 300 SNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTL 359

Query: 181 EFAKI 183
           + AKI
Sbjct: 360 KLAKI 364

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BLAST of HG10019837 vs. NCBI nr
Match: XP_042959872.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like [Carya illinoinensis] >KAG6630128.1 hypothetical protein CIPAW_14G134500 [Carya illinoinensis])

HSP 1 Score: 245.0 bits (624), Expect = 5.1e-61
Identity = 111/187 (59.36%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+KSSE +IKR+L++LM  LN NE+D+T       S+ I  NYYPICP P+L VGVGR
Sbjct: 174 LEYMKSSEVVIKRLLEVLMKGLNVNEIDETKESLLMGSKRINLNYYPICPNPELTVGVGR 233

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD++GGL++ RG +D W+HV P++ SLVIN+GD+++IMSNG YKS+EH 
Sbjct: 234 HSDVSTLTILLQDEIGGLYV-RGKNDSWIHVPPISGSLVINVGDALQIMSNGRYKSIEHR 293

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+ +R+S+PIF+NPK   I+ P PEVLA G+KA Y++V++SDY  HFF K HDGKN
Sbjct: 294 VLANGSQNRISVPIFVNPKPCEIIGPFPEVLAGGEKAAYRQVLYSDYVKHFFRKAHDGKN 353

Query: 181 TIEFAKI 183
           T+EFAKI
Sbjct: 354 TVEFAKI 359

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BLAST of HG10019837 vs. NCBI nr
Match: KAG6679526.1 (hypothetical protein I3842_14G136700 [Carya illinoinensis] >KAG7948178.1 hypothetical protein I3843_14G135300 [Carya illinoinensis])

HSP 1 Score: 244.6 bits (623), Expect = 6.7e-61
Identity = 111/187 (59.36%), Postives = 150/187 (80.21%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+KSSE +IKR+L++LM  LN NE+D+T       S+ I  NYYPICP P+L VGVGR
Sbjct: 174 LEYMKSSEVVIKRLLEVLMKGLNVNEIDETKESLLMGSKRINLNYYPICPNPELTVGVGR 233

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD++GGL++ RG +D W+HV P+  SLVIN+GD+++IMSNG YKS+EH 
Sbjct: 234 HSDVSTLTILLQDEIGGLYV-RGKNDSWIHVPPICGSLVINVGDALQIMSNGRYKSIEHR 293

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+ +R+S+PIF+NPK   I+ P PEVLA G+KA Y++V++SDY  HFF K HDGKN
Sbjct: 294 VLANGSQNRISVPIFVNPKPCEIIGPFPEVLAGGEKAAYRQVLYSDYVKHFFRKAHDGKN 353

Query: 181 TIEFAKI 183
           T+EFAKI
Sbjct: 354 TVEFAKI 359

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BLAST of HG10019837 vs. NCBI nr
Match: XP_040994549.1 (feruloyl CoA ortho-hydroxylase F6H1-2-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 241.5 bits (615), Expect = 5.6e-60
Identity = 109/187 (58.29%), Postives = 150/187 (80.21%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+KSSE +IKR+L++LM  LN NE+D+        S+ I  NYYPICP P+L VGVGR
Sbjct: 173 LEYMKSSEVVIKRLLEVLMKRLNVNEIDEMKESLLMGSKRINLNYYPICPNPELTVGVGR 232

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD++GGL++ RG +D W+HV P++ SLVIN+GD+++IMSNG YKS+EH 
Sbjct: 233 HSDVSTLTILLQDEIGGLYV-RGKNDSWIHVPPISGSLVINVGDALQIMSNGRYKSIEHR 292

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+ +R+S+PIF+NPK   I+ P PE+LA G+KA Y++V++SDY  HFF K HDGKN
Sbjct: 293 VLANGSQNRISVPIFVNPKPYEIIGPFPEMLAGGEKAAYRQVLYSDYVKHFFGKAHDGKN 352

Query: 181 TIEFAKI 183
           T+EFAKI
Sbjct: 353 TVEFAKI 358

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BLAST of HG10019837 vs. NCBI nr
Match: XP_008218314.1 (PREDICTED: feruloyl CoA ortho-hydroxylase 1-like [Prunus mume])

HSP 1 Score: 240.7 bits (613), Expect = 9.6e-60
Identity = 114/188 (60.64%), Postives = 148/188 (78.72%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+K+SE LIKR+L +LM  LN  E+D+        SR I  NYYPICP P+L VGVGR
Sbjct: 172 LEYMKNSEILIKRLLDMLMKRLNVKEIDQEKEGLLMGSRRINLNYYPICPNPELTVGVGR 231

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDE-WVHVKPVAESLVINIGDSMEIMSNGVYKSVEH 120
           HSD+S +TVLLQD +GGL++R G D + W+HV PV  SLVINIGD+++IMSNG YKS+EH
Sbjct: 232 HSDVSTLTVLLQDQIGGLYVRGGTDKKSWIHVPPVKGSLVINIGDALQIMSNGRYKSIEH 291

Query: 121 CVVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGK 180
            V ANG+ +R+S+PIF+NP+ S ++ PLPEVLA+G+KA YK+V++SDY  HFF K HDGK
Sbjct: 292 RVAANGSKNRISVPIFVNPRPSDLISPLPEVLASGEKAVYKQVLYSDYVKHFFRKAHDGK 351

Query: 181 NTIEFAKI 183
           +TIEFAKI
Sbjct: 352 STIEFAKI 359

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BLAST of HG10019837 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 8.5e-59
Identity = 103/187 (55.08%), Postives = 142/187 (75.94%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNRSR-----TIQFNYYPICPTPDLNVGVGR 60
           ++Y+  S+ +++R+L+ L  NLN  ELD+T  S       +  NYYPICP PDL VGVGR
Sbjct: 175 LEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGR 234

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD +GGLH+R      WVHV PVA S VINIGD+M+IMSNG+YKSVEH 
Sbjct: 235 HSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHR 294

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG ++R+S+PIF+NPK  +++ PLPEV+A G++  Y++V++SDY  +FF K HDGK 
Sbjct: 295 VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKK 354

Query: 181 TIEFAKI 183
           T+++AKI
Sbjct: 355 TVDYAKI 361

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BLAST of HG10019837 vs. ExPASy Swiss-Prot
Match: G9M9M0 (Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 4.2e-58
Identity = 99/187 (52.94%), Postives = 144/187 (77.01%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           + ++++ + +IKR+LK LM  LN  E+D T       S+ I  NYYP CP P+L VGVGR
Sbjct: 171 LTFMRNCDAVIKRLLKSLMKGLNVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGR 230

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD +GGL++R+   DEWVHV P+  ++VIN+GD+++I+SNG YKS+EH 
Sbjct: 231 HSDVSTLTILLQDQIGGLYVRKLDSDEWVHVPPINGAIVINVGDALQILSNGRYKSIEHR 290

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+++R+S+PIF+NP+ + ++ PLPE+L +G+KA YK V++SDY  HFF K HDGK 
Sbjct: 291 VIANGSNNRISVPIFVNPRPNDVIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKE 350

Query: 181 TIEFAKI 183
           T++FAKI
Sbjct: 351 TVDFAKI 357

[Close]

BLAST of HG10019837 vs. ExPASy Swiss-Prot
Match: G9M9M4 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomoea batatas OX=4120 GN=F6H2-1-1 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.2e-58
Identity = 107/187 (57.22%), Postives = 142/187 (75.94%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++YLKS E + +++L+ LM  LN NE+D         SR I  NYYP CP PDL VGVGR
Sbjct: 171 LEYLKSCEMVSRKLLEALMQGLNVNEIDDAKESLLMGSRRININYYPKCPNPDLTVGVGR 230

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSDIS +T+LLQDD+GGL++R+   + W HV PV  +LVINIGD+++IMSNG YKS+EH 
Sbjct: 231 HSDISTLTLLLQDDIGGLYVRKLEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHR 290

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+AN  +DR+S+P+F+NPK + IV PLPEVLA+G+K  YK V++SDYA HF+ K H+GK+
Sbjct: 291 VLANETNDRISVPVFVNPKPNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKD 350

Query: 181 TIEFAKI 183
           TI FA+I
Sbjct: 351 TIAFARI 357

[Close]

BLAST of HG10019837 vs. ExPASy Swiss-Prot
Match: G9M9M5 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomoea batatas OX=4120 GN=F6H2-1-8 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 9.4e-58
Identity = 106/187 (56.68%), Postives = 144/187 (77.01%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++YLKS E + +++L+ LM  LN N++D +       SR I  NYYP CP PDL VGVGR
Sbjct: 172 LEYLKSCELVSRKLLEALMQGLNVNQIDDSKESLLMGSRRININYYPKCPNPDLTVGVGR 231

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSDIS +T+LLQDD+GGL++R+   + W HV PV  +LVINIGD+++IMSNG YKS+EH 
Sbjct: 232 HSDISTLTLLLQDDIGGLYVRKLEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHR 291

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+AN ++DR+S+P+F+NPK + IV PLPEVLA+G+K  YK V++SDYA HF+ K H+GK+
Sbjct: 292 VMANESNDRISVPVFVNPKPNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKD 351

Query: 181 TIEFAKI 183
           TI FA+I
Sbjct: 352 TIAFARI 358

[Close]

BLAST of HG10019837 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.6e-57
Identity = 99/187 (52.94%), Postives = 143/187 (76.47%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           + ++++ + +IKR+LK LM  LN  E+D T       S+ I  NYYP CP P+L VGVGR
Sbjct: 171 LTFMRNCDAVIKRLLKSLMKGLNVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGR 230

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD +GGL++R+   D WVHV P+  ++VIN+GD+++I+SNG YKS+EH 
Sbjct: 231 HSDVSTLTILLQDQIGGLYVRKLDSDTWVHVPPINGAIVINVGDALQILSNGRYKSIEHR 290

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+++R+S+PIF+NP+ + I+ PLPE+L +G+KA YK V++SDY  HFF K HDGK 
Sbjct: 291 VIANGSNNRISVPIFVNPRPNDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKE 350

Query: 181 TIEFAKI 183
           T++FAKI
Sbjct: 351 TVDFAKI 357

[Close]

BLAST of HG10019837 vs. ExPASy TrEMBL
Match: A0A0A0LFH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G821020 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 6.3e-73
Identity = 140/185 (75.68%), Postives = 162/185 (87.57%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNRSRTIQFNYYPICPTPDLNVGVGRHSDIS 60
           ++YLKSS TLIKRVLKILMN LNANELD+TN++R IQFNYYPI PTPDL+VGV  HSD+S
Sbjct: 180 IEYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVS 239

Query: 61  LVTVLLQDDVGGLHMRRGID---DEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHCVV 120
           L+TVLLQDDVGGLH+RR ID   DEWV VKPVAESLVINIGD +EIMSNG+YKSVEHCVV
Sbjct: 240 LLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVV 299

Query: 121 ANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKNTI 180
           +NGN DRMSIPIF+  ++S  + PLPEVLATGKKA+YKEVVFSDY  +F +KP DGK+T+
Sbjct: 300 SNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTL 359

Query: 181 EFAKI 183
           + AKI
Sbjct: 360 KLAKI 364

[Close]

BLAST of HG10019837 vs. ExPASy TrEMBL
Match: A0A2I4EDQ8 (feruloyl CoA ortho-hydroxylase F6H1-2-like OS=Juglans regia OX=51240 GN=LOC108988663 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 7.9e-60
Identity = 108/187 (57.75%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+KSSE +IKR+L++LM  LN NE+D+        S+ I  NYYPICP P+L VGVGR
Sbjct: 173 LEYMKSSEVVIKRLLEVLMKRLNVNEIDEMKESLLMGSKRINLNYYPICPNPELTVGVGR 232

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD++GGL++ RG +D W+HV P++ SLVIN+GD+++IMSNG YKS+EH 
Sbjct: 233 HSDVSTLTILLQDEIGGLYV-RGKNDSWIHVPPISGSLVINVGDALQIMSNGRYKSIEHR 292

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+ +R+S+PIF+NPK   I+ P PE+L  G+KA Y++V++SDY  HFF K HDGKN
Sbjct: 293 VLANGSQNRISVPIFVNPKPYEIIGPFPEMLTGGEKAAYRQVLYSDYVKHFFGKAHDGKN 352

Query: 181 TIEFAKI 183
           T+EFAKI
Sbjct: 353 TVEFAKI 358

[Close]

BLAST of HG10019837 vs. ExPASy TrEMBL
Match: A0A7J0H655 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_26g0015130 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.0e-59
Identity = 109/187 (58.29%), Postives = 148/187 (79.14%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++++K SE +IKR+L+ LM  LN  +LD+T       S+ I  NYYPICP P+L VGVGR
Sbjct: 118 LEFMKKSEFVIKRLLEALMRRLNVKDLDETKELLLMGSKRINLNYYPICPNPELTVGVGR 177

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +TVLLQDD+GGL++R+  DD W+HV PVA SLVINIGD+++IMSNG YKS+EH 
Sbjct: 178 HSDVSTLTVLLQDDIGGLYVRKIADDSWIHVPPVAGSLVINIGDALQIMSNGRYKSIEHR 237

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V ANG+ +R+SIPIF+NP+ S ++ PLPEV+  G+K +Y++V++SDY  +FF K HDGK+
Sbjct: 238 VTANGSKNRVSIPIFMNPRPSEVIGPLPEVIERGEKPEYRQVLYSDYVKYFFKKAHDGKD 297

Query: 181 TIEFAKI 183
           T+EFAKI
Sbjct: 298 TVEFAKI 304

[Close]

BLAST of HG10019837 vs. ExPASy TrEMBL
Match: M5W6M1 (Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G006000 PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.8e-59
Identity = 113/188 (60.11%), Postives = 147/188 (78.19%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+K SE LIKR+L++LM  LN  E+D+        S  I  NYYPICP P+L VGVGR
Sbjct: 172 LEYMKKSEILIKRLLEMLMKRLNVKEIDQEKEGLLMGSMRINLNYYPICPNPELTVGVGR 231

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDE-WVHVKPVAESLVINIGDSMEIMSNGVYKSVEH 120
           HSD+S +TVLLQD +GGL++R G D + W+HV PV  SLVINIGD+++IMSNG YKS+EH
Sbjct: 232 HSDVSTLTVLLQDQIGGLYVRGGTDKKTWIHVPPVKGSLVINIGDALQIMSNGRYKSIEH 291

Query: 121 CVVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGK 180
            V ANG+ +R+S+PIF+NP+ S ++ PLPEVLA+G+KA YK+V++SDY  HFF K HDGK
Sbjct: 292 RVAANGSKNRISVPIFVNPRPSDLISPLPEVLASGEKAVYKQVLYSDYVKHFFRKAHDGK 351

Query: 181 NTIEFAKI 183
           +TIEFAKI
Sbjct: 352 STIEFAKI 359

[Close]

BLAST of HG10019837 vs. ExPASy TrEMBL
Match: A0A6J5XFQ0 (Fe2OG dioxygenase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34993 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.3e-59
Identity = 113/188 (60.11%), Postives = 146/188 (77.66%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+K SE LIKR+L++LM  LN  E+D+        S  I  NYYPICP P+L VGVGR
Sbjct: 178 LEYMKKSEILIKRLLEMLMKRLNVKEIDQEKEGLLMGSMRINLNYYPICPNPELTVGVGR 237

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDE-WVHVKPVAESLVINIGDSMEIMSNGVYKSVEH 120
           HSD+S +TVLLQD +GGL++R G D + W+HV PV  SLVINIGD+++IMSNG YKS+EH
Sbjct: 238 HSDVSTLTVLLQDQIGGLYVRGGTDKKSWIHVPPVKGSLVINIGDALQIMSNGRYKSIEH 297

Query: 121 CVVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGK 180
            V ANG+ +R+S+PIF+NP+ S ++ PLPEVLA G+KA YK+V++SDY  HFF K HDGK
Sbjct: 298 RVAANGSKNRISVPIFVNPRPSDLISPLPEVLANGEKAVYKQVLYSDYVKHFFRKAHDGK 357

Query: 181 NTIEFAKI 183
           +TIEFAKI
Sbjct: 358 STIEFAKI 365

[Close]

BLAST of HG10019837 vs. TAIR 10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 6.0e-60
Identity = 103/187 (55.08%), Postives = 142/187 (75.94%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNRSR-----TIQFNYYPICPTPDLNVGVGR 60
           ++Y+  S+ +++R+L+ L  NLN  ELD+T  S       +  NYYPICP PDL VGVGR
Sbjct: 175 LEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGR 234

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD +GGLH+R      WVHV PVA S VINIGD+M+IMSNG+YKSVEH 
Sbjct: 235 HSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHR 294

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG ++R+S+PIF+NPK  +++ PLPEV+A G++  Y++V++SDY  +FF K HDGK 
Sbjct: 295 VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKK 354

Query: 181 TIEFAKI 183
           T+++AKI
Sbjct: 355 TVDYAKI 361

[Close]

BLAST of HG10019837 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.1e-58
Identity = 102/187 (54.55%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGR 60
           ++Y+  ++ L+K++L+ L  NLN  ELDKT       S  I  NYYPICP P+L VGVGR
Sbjct: 175 LEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTVGVGR 234

Query: 61  HSDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHC 120
           HSD+S +T+LLQD++GGLH+R      WVHV P++ SLVINIGD+M+IMSNG YKSVEH 
Sbjct: 235 HSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLVINIGDAMQIMSNGRYKSVEHR 294

Query: 121 VVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKN 180
           V+ANG+ +R+S+PIF++PK  +++ PL EV+  G+K  YK+++++DY  HFF K HDGK 
Sbjct: 295 VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAHDGKK 354

Query: 181 TIEFAKI 183
           TI+FA I
Sbjct: 355 TIDFANI 361

[Close]

BLAST of HG10019837 vs. TAIR 10
Match: AT5G12270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 4.2e-45
Identity = 87/189 (46.03%), Postives = 129/189 (68.25%), Query Frame = 0


Query: 3   YLKSSETLIKRVLKILMNNLNANELDKTNR-----SRTIQFNYYPICPTPDLNVGVGRHS 62
           + K  + + +R+L IL+NNLN   +D++N+     +  + FNYYP CP P L +G GRHS
Sbjct: 172 HFKRIKPITERILNILINNLN-TIIDESNKETLMGTMRMNFNYYPKCPEPSLAIGTGRHS 231

Query: 63  DISLVTVLLQDD--VGGLHMRRGID-DEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEH 122
           DI+ +T+LLQ+D  +  L+ R   D D+W+HV P+  ++V+NIGD ++I+SN  Y+SVEH
Sbjct: 232 DINTLTLLLQEDGVLSSLYARATEDGDKWIHVPPIPGAIVVNIGDVLQILSNDRYRSVEH 291

Query: 123 CVVANGNSDRMSIPIFINPKHSAIVRPLPEVL-ATGKKAQYKEVVFSDYATHFFSKPHDG 182
           CVV N    R+SIP+F  P H +++ PLPEVL    + A+Y+++V+SDY   FF +PHDG
Sbjct: 292 CVVVNKYCSRVSIPVFCGPVHDSVIEPLPEVLDKNNEMARYRKIVYSDYLKFFFGRPHDG 351

[Close]

BLAST of HG10019837 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 7.1e-45
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 1   MKYLKSSETLIKRVLKILMNNLNAN-ELDKTN---RSRTIQFNYYPICPTPDLNVGVGRH 60
           +++L SS  ++K V+ ILM N+    E +K N    ++ +  NYYP CP+P+L VGVGRH
Sbjct: 172 LEFLNSSMEMVKNVVNILMENVGVTLEEEKMNGLMGTKMVNMNYYPTCPSPELTVGVGRH 231

Query: 61  SDISLVTVLLQDDVGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKSVEHCV 120
           SD+ ++TVLLQD +GGL+++   + EW  + PV  +LVINIGD+++I+SNG YKS EH V
Sbjct: 232 SDMGMLTVLLQDGIGGLYVKLD-NGEWAEIPPVHGALVINIGDTLQILSNGKYKSAEHRV 291

Query: 121 VANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPHDGKNT 180
                  R+S+PIF  P  S  V PLPEV+     A+YKE +F DY  +FF +PHDGK +
Sbjct: 292 RTTNIGSRVSVPIFTAPNPSQKVGPLPEVVKRDGVARYKEFLFQDYMNNFFGQPHDGKKS 351

Query: 181 IEFAK 182
           ++FA+
Sbjct: 352 LDFAR 355

[Close]

BLAST of HG10019837 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.5e-30
Identity = 71/187 (37.97%), Postives = 108/187 (57.75%), Query Frame = 0


Query: 2   KYLKSSETLIKRVLKILMNN--LNANEL-------DKTNRSRTIQFNYYPICPTPDLNVG 61
           KY +    L +R+ + L  +  L  N+L       DK   S  ++ N+YP CP P L +G
Sbjct: 165 KYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGAS--LRTNFYPKCPQPQLTLG 224

Query: 62  VGRHSDISLVTVLLQDD-VGGLHMRRGIDDEWVHVKPVAESLVINIGDSMEIMSNGVYKS 121
           +  HSD   +T+LL D+ V GL +RRG  D WV +K V  +L++NIGD ++I+SNG+YKS
Sbjct: 225 LSSHSDPGGITILLPDEKVAGLQVRRG--DGWVTIKSVPNALIVNIGDQLQILSNGIYKS 284

Query: 122 VEHCVVANGNSDRMSIPIFINPKHSAIVRPLPEVLATGKKAQYKEVVFSDYATHFFSKPH 179
           VEH V+ N   +R+S+  F NP+    V P+ E++   + A YK + F +Y +    K  
Sbjct: 285 VEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGP 344

The following BLAST results are available for this feature:

BLAST of HG10019837 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652189.1	1.3e-72	75.68	feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical prot...	[more]
XP_042959872.1	5.1e-61	59.36	feruloyl CoA ortho-hydroxylase F6H1-3-like [Carya illinoinensis] >KAG6630128.1 h...	[more]
KAG6679526.1	6.7e-61	59.36	hypothetical protein I3842_14G136700 [Carya illinoinensis] >KAG7948178.1 hypothe...	[more]
XP_040994549.1	5.6e-60	58.29	feruloyl CoA ortho-hydroxylase F6H1-2-like [Juglans microcarpa x Juglans regia]	[more]
XP_008218314.1	9.6e-60	60.64	PREDICTED: feruloyl CoA ortho-hydroxylase 1-like [Prunus mume]	[more]

BLAST of HG10019837 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHN8	8.5e-59	55.08	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
G9M9M0	4.2e-58	52.94	Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 ...	[more]
G9M9M4	7.2e-58	57.22	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomo...	[more]
G9M9M5	9.4e-58	56.68	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomo...	[more]
G9M9M1	1.6e-57	52.94	Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ...	[more]

BLAST of HG10019837 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFH9	6.3e-73	75.68	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A2I4EDQ8	7.9e-60	57.75	feruloyl CoA ortho-hydroxylase F6H1-2-like OS=Juglans regia OX=51240 GN=LOC10898...	[more]
A0A7J0H655	1.0e-59	58.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein OS=Actin...	[more]
M5W6M1	1.8e-59	60.11	Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6...	[more]
A0A6J5XFQ0	2.3e-59	60.11	Fe2OG dioxygenase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAR...	[more]

BLAST of HG10019837 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13610.1	6.0e-60	55.08	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G55290.1	1.1e-58	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G12270.1	4.2e-45	46.03	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G12900.1	7.1e-45	47.03	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G38240.1	6.5e-30	37.97	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 56..77 score: 43.8 coord: 121..139 score: 34.45
None	No IPR available	PANTHER	PTHR10209:SF764	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE-RELATED	coord: 2..174
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 2..174
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..174
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..165 e-value: 2.5E-51 score: 177.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 38..133 e-value: 2.0E-24 score: 86.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 32..133 score: 13.061053

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019837.1	HG10019837.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen