HG10019764 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10019764
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCalcium-binding EF-hand family protein
LocationChr04: 25263902 .. 25264396 (+)
RNA-Seq ExpressionHG10019764
SyntenyHG10019764
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCCTCAAATCCCTCAATCCCAATCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCTTCCTTCGGCTCCGCCTCCTCCTCTTCCTCCGCCGGCACCCCAACCAGCGTCTTGCCCTCCGCCGACTATTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTCGAGGCTTTACTCGGCCTAATCGGAGCCCAACCACCGAGTGAGGAAGAGCTCAAGATTATGATGGGCGAAATGGACCGGGTCGTACCGGCTTGCCACTCCGAGCTACGAGACACGTTTGAGATATTCGACGCTGACCACGACGGCAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAGAATGGGGATGGGTTCGTCTGCTTCGACGAGTTTGCGCGTATGATGGACTGCCAGAGATGA

mRNA sequence

ATGAAGCTCCTCAAATCCCTCAATCCCAATCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCTTCCTTCGGCTCCGCCTCCTCCTCTTCCTCCGCCGGCACCCCAACCAGCGTCTTGCCCTCCGCCGACTATTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTCGAGGCTTTACTCGGCCTAATCGGAGCCCAACCACCGAGTGAGGAAGAGCTCAAGATTATGATGGGCGAAATGGACCGGGTCGTACCGGCTTGCCACTCCGAGCTACGAGACACGTTTGAGATATTCGACGCTGACCACGACGGCAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAGAATGGGGATGGGTTCGTCTGCTTCGACGAGTTTGCGCGTATGATGGACTGCCAGAGATGA

Coding sequence (CDS)

ATGAAGCTCCTCAAATCCCTCAATCCCAATCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCTTCCTTCGGCTCCGCCTCCTCCTCTTCCTCCGCCGGCACCCCAACCAGCGTCTTGCCCTCCGCCGACTATTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTCGAGGCTTTACTCGGCCTAATCGGAGCCCAACCACCGAGTGAGGAAGAGCTCAAGATTATGATGGGCGAAATGGACCGGGTCGTACCGGCTTGCCACTCCGAGCTACGAGACACGTTTGAGATATTCGACGCTGACCACGACGGCAGAATTACGGCGGAGGAGCTTTTTAGTGTGTTCGCGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCCGGAGTGGACAAGAATGGGGATGGGTTCGTCTGCTTCGACGAGTTTGCGCGTATGATGGACTGCCAGAGATGA

Protein sequence

MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSADYSDFSDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR
Homology
BLAST of HG10019764 vs. NCBI nr
Match: XP_038903370.1 (probable calcium-binding protein CML36 [Benincasa hispida])

HSP 1 Score: 300.1 bits (767), Expect = 1.2e-77
Identity = 154/164 (93.90%), Postives = 158/164 (96.34%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSADYSDFSDSTLS 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSA +SDFSDSTLS
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDFSDSTLS 60

Query: 61  RKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITAEELF 120
           RKELEALLGLIGA+PPSEEELK MMG MDRV PACHSELRDTFEIFDADHDGRITAEELF
Sbjct: 61  RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAEELF 120

Query: 121 SVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           SVF AMGDEQCTLEDCRRMIAGVDKNGDGFVCF++FARMMDCQR
Sbjct: 121 SVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 164

BLAST of HG10019764 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa] >TYK25587.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa])

HSP 1 Score: 293.5 bits (750), Expect = 1.1e-75
Identity = 152/168 (90.48%), Postives = 158/168 (94.05%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSADYSDFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS D++DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGA+PPSEEE+KIMMGEMDRV PACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF AMGDE CTLEDCRRMIAGVDKNGDGFVCFD+FARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

BLAST of HG10019764 vs. NCBI nr
Match: XP_004136671.2 (probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetical protein Csa_001340 [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 2.5e-75
Identity = 151/167 (90.42%), Postives = 158/167 (94.61%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS---AGTPTSVLPSADYSDFSDS 60
           MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS   AGTPTSVLPS D+SDFSD 
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITAE 120
           TLSRKEL+ALLGLIGA+PPSEEE+KIMMGEMDRV P CHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           ELFSVFAAMGD+ CTLEDC+RMIAGVDKNGDGFVCFD+F RMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of HG10019764 vs. NCBI nr
Match: KAG6580931.1 (putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017674.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.5 bits (615), Expect = 5.1e-60
Identity = 128/168 (76.19%), Postives = 144/168 (85.71%), Query Frame = 0

Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SADYSDFSDS 64
           KS +P RLF SK HR+ LS+S+PPSFGSASSSS    PTSVLP       S ++SDFSD+
Sbjct: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSS----PTSVLPPHPHPHLSGEWSDFSDT 68

Query: 65  -TLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 124
            T  RKELEALLGLIGA+PPSEEELK+MM ++D+V P CHSELRDTFEIFDADHDGRITA
Sbjct: 69  HTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITA 128

Query: 125 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCF++FA MM+CQR
Sbjct: 129 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 172

BLAST of HG10019764 vs. NCBI nr
Match: XP_022983878.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 241.5 bits (615), Expect = 5.1e-60
Identity = 127/168 (75.60%), Postives = 144/168 (85.71%), Query Frame = 0

Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SADYSDFSDS 64
           KS +P RLF SK HR+ LS+S+PPSFGSAS SS    PTSVLP       S ++SDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  TLS-RKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 124
            +  RKELEALLGLIGA+PPSEEELK+MM ++D+V PACHSELRDTFEIFDADHDGRITA
Sbjct: 81  HIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCF++FA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

BLAST of HG10019764 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-30
Identity = 97/210 (46.19%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPP-SFGSASSSS------SAG-------TPTSVL 60
           MKL K L P R F     RS +S+S    SFGSASSSS      S G       TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSA----DYSDF----------SDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMD--- 120
           P       Y +           +D  +SR +LE+LL  +G  P +EEE+ +M+ E+D   
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -----------RVV---PACHS-ELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLE 165
                      RVV   PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE+CTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

BLAST of HG10019764 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 4.4e-30
Identity = 95/208 (45.67%), Postives = 120/208 (57.69%), Query Frame = 0

Query: 1   MKLLKSLN---PNRLFSSKSHRSKLSRSQPPSFGS-ASSSSSAG-----------TPTSV 60
           MKL  SLN   P RLF +KS ++ +SRS+P SF S ASSSSS G           TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LP--SAD-YSDF----------SDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVV 120
           LP  S D Y++            D  +SR +L AL+  +  +PPS+EE+ +M+ E+D   
Sbjct: 61  LPQNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGD 120

Query: 121 PACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCT 161
             C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE+CT
Sbjct: 121 GGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCT 180

BLAST of HG10019764 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.7e-14
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 0

Query: 56  DSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPA-------------------CH 115
           D  +S KEL A++  +G + P EEEL+  + E+DR                         
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 116 SELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEF 161
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + F+EF
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

BLAST of HG10019764 vs. ExPASy Swiss-Prot
Match: Q5ZCK5 (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.7e-13
Identity = 52/128 (40.62%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 56  DSTLSRKELEALLGLIGAQPPSE----EELKIMMGEMD-------------------RVV 115
           D  +S  EL A+   I A PPSE     E+  MM E+D                   R  
Sbjct: 40  DGRISPSELAAVTRAI-APPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRGRGD 99

Query: 116 PACHSELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVC 161
               +ELR  F+++D D DGRITA EL  V   +G E C+ E+C RMIA VD +GDG V 
Sbjct: 100 AEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDGCVG 159

BLAST of HG10019764 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-12
Identity = 49/141 (34.75%), Postives = 69/141 (48.94%), Query Frame = 0

Query: 51  YSDFSDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDR-------------------- 110
           Y    D  +S +EL ++L  +GA P    E++ MM EMD                     
Sbjct: 13  YDANGDGKISAEELASVLRALGA-PLGPGEVRRMMDEMDSDRDGFVDLSEFAAFHCGPTP 72

Query: 111 -----------VVPACHSELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRM 161
                         A  +ELR+ F ++DAD +G+I+A EL  V   +GD +C++ DC RM
Sbjct: 73  AHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGD-KCSVADCSRM 132

BLAST of HG10019764 vs. ExPASy TrEMBL
Match: A0A5A7UEZ0 (Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007460 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.5e-76
Identity = 152/168 (90.48%), Postives = 158/168 (94.05%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSADYSDFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS D++DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGA+PPSEEE+KIMMGEMDRV PACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF AMGDE CTLEDCRRMIAGVDKNGDGFVCFD+FARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

BLAST of HG10019764 vs. ExPASy TrEMBL
Match: A0A1S3B7R8 (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.5e-76
Identity = 152/168 (90.48%), Postives = 158/168 (94.05%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSADYSDFSD 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS D++DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGA+PPSEEE+KIMMGEMDRV PACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF AMGDE CTLEDCRRMIAGVDKNGDGFVCFD+FARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

BLAST of HG10019764 vs. ExPASy TrEMBL
Match: A0A0A0LFY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.2e-75
Identity = 151/167 (90.42%), Postives = 158/167 (94.61%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS---AGTPTSVLPSADYSDFSDS 60
           MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS   AGTPTSVLPS D+SDFSD 
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITAE 120
           TLSRKEL+ALLGLIGA+PPSEEE+KIMMGEMDRV P CHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           ELFSVFAAMGD+ CTLEDC+RMIAGVDKNGDGFVCFD+F RMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

BLAST of HG10019764 vs. ExPASy TrEMBL
Match: A0A6J1J8Y7 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111482360 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.5e-60
Identity = 127/168 (75.60%), Postives = 144/168 (85.71%), Query Frame = 0

Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SADYSDFSDS 64
           KS +P RLF SK HR+ LS+S+PPSFGSAS SS    PTSVLP       S ++SDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  TLS-RKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 124
            +  RKELEALLGLIGA+PPSEEELK+MM ++D+V PACHSELRDTFEIFDADHDGRITA
Sbjct: 81  HIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCF++FA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

BLAST of HG10019764 vs. ExPASy TrEMBL
Match: A0A6J1FB49 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111442449 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.5e-60
Identity = 128/168 (76.19%), Postives = 144/168 (85.71%), Query Frame = 0

Query: 5   KSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SADYSDFSDS 64
           KS +P RLF SK HR+ LS+S+PPSFGSASSSS    PTSVLP       S ++SDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  -TLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPACHSELRDTFEIFDADHDGRITA 124
            T  RKELEALLGLIGA+PPSEEELK+MM ++D+V P CHSELRDTFEIFDADHDGRITA
Sbjct: 81  HTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCF++FA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

BLAST of HG10019764 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.1e-31
Identity = 97/210 (46.19%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPP-SFGSASSSS------SAG-------TPTSVL 60
           MKL K L P R F     RS +S+S    SFGSASSSS      S G       TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSA----DYSDF----------SDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMD--- 120
           P       Y +           +D  +SR +LE+LL  +G  P +EEE+ +M+ E+D   
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -----------RVV---PACHS-ELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLE 165
                      RVV   PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE+CTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

BLAST of HG10019764 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 132.5 bits (332), Expect = 3.1e-31
Identity = 95/208 (45.67%), Postives = 120/208 (57.69%), Query Frame = 0

Query: 1   MKLLKSLN---PNRLFSSKSHRSKLSRSQPPSFGS-ASSSSSAG-----------TPTSV 60
           MKL  SLN   P RLF +KS ++ +SRS+P SF S ASSSSS G           TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LP--SAD-YSDF----------SDSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVV 120
           LP  S D Y++            D  +SR +L AL+  +  +PPS+EE+ +M+ E+D   
Sbjct: 61  LPQNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGD 120

Query: 121 PACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCT 161
             C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE+CT
Sbjct: 121 GGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCT 180

BLAST of HG10019764 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 79.0 bits (193), Expect = 4.1e-15
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 0

Query: 56  DSTLSRKELEALLGLIGAQPPSEEELKIMMGEMDRVVPA-------------------CH 115
           D  +S KEL A++  +G + P EEEL+  + E+DR                         
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 116 SELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEF 161
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + F+EF
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

BLAST of HG10019764 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 72.8 bits (177), Expect = 2.9e-13
Identity = 45/127 (35.43%), Postives = 71/127 (55.91%), Query Frame = 0

Query: 55  SDSTLSRKELEALLGLIGAQPPSEEELKIMMGE----------MDRVVP----------- 114
           +D  +S  EL+ ++G +     S+EE K MM E          +D  V            
Sbjct: 27  NDGKISIDELKDVIGALSPN-ASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNN 86

Query: 115 ACHSELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCF 161
           +   +L++ F+++D D +GRI+A EL SV   +G E+C+++DC+RMI  VD +GDG V F
Sbjct: 87  SAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVDF 146

BLAST of HG10019764 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 72.8 bits (177), Expect = 2.9e-13
Identity = 33/64 (51.56%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 97  SELRDTFEIFDADHDGRITAEELFSVFAAMGDEQCTLEDCRRMIAGVDKNGDGFVCFDEF 156
           S+L++ FE++D D +GRI+A+EL SV   +G E+C+++DC++MI+ VD +GDG V FDEF
Sbjct: 93  SDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGCVNFDEF 152

Query: 157 ARMM 161
            +MM
Sbjct: 153 KKMM 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903370.11.2e-7793.90probable calcium-binding protein CML36 [Benincasa hispida][more]
XP_008443339.21.1e-7590.48PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 p... [more]
XP_004136671.22.5e-7590.42probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetica... [more]
KAG6580931.15.1e-6076.19putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022983878.15.1e-6075.60probable calcium-binding protein CML36 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SS311.5e-3046.19Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ... [more]
P301884.4e-3045.67Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ... [more]
Q9FYK25.7e-1437.90Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q5ZCK53.7e-1340.63Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q0DJV61.8e-1234.75Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UEZ05.5e-7690.48Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B7R85.5e-7690.48probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P... [more]
A0A0A0LFY71.2e-7590.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1[more]
A0A6J1J8Y72.5e-6075.60probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114823... [more]
A0A6J1FB492.5e-6076.19probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT3G10190.11.1e-3146.19Calcium-binding EF-hand family protein [more]
AT2G41410.13.1e-3145.67Calcium-binding EF-hand family protein [more]
AT1G24620.14.1e-1537.90EF hand calcium-binding protein family [more]
AT1G66400.12.9e-1335.43calmodulin like 23 [more]
AT5G37770.12.9e-1351.56EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 135..163
e-value: 3.2E-4
score: 30.0
coord: 98..126
e-value: 3.4E-5
score: 33.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 98..160
e-value: 1.7E-9
score: 38.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 94..129
score: 12.867577
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 131..164
score: 12.225983
IPR002048EF-hand domainCDDcd00051EFhcoord: 98..160
e-value: 6.90799E-19
score: 73.7361
NoneNo IPR availableGENE3D1.10.238.10coord: 94..164
e-value: 5.2E-21
score: 77.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..49
NoneNo IPR availablePANTHERPTHR10891:SF790CALCIUM-BINDING PROTEIN CML35-RELATEDcoord: 4..89
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 95..162
coord: 4..89
NoneNo IPR availablePANTHERPTHR10891:SF790CALCIUM-BINDING PROTEIN CML35-RELATEDcoord: 95..162
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 107..119
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 144..156
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 55..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10019764.1HG10019764.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding