HG10019722 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019722
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Ribonuclease T(2)
Location	Chr04: 24839687 .. 24840476 (+)
RNA-Seq Expression	HG10019722
Synteny	HG10019722

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACAACAAAGAGAGATATTGTCTTCTTAGTGTTTGCATTAACTATTTTGTTTCCTATTGTTAAATCACAAACTTTTGATGACTTTTGGTTTGTACAACAATGGCCACCTGCTGTTTGCGCTTCACAATCAGGACGATGTGTAGGAAGAGGGACGCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAAGGAGGAAACTCTGTGATTAATTGCGGTGGCACTCCCTTTGATTTCACTAAGGTAAAACTTAAACACTCAAAAGTCTCTAATTCTTCTTATTAATCATGCATCCATTCTAACTATGAACCATTATGAGTATATAATTAAGTGTTCAACTGGTTAATGATCTAATCTTGTACTTATTATGTGGATGTTTCAGATTGCACATTTAGAAAATGATCTAAACGTAGTTTGGCCAAATGTGGTAACGGGAGACCACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGTTCGGAAAGCAAGTTTGACATGCCAAAGTACTTCCAAACAGCAATAAACATGAGGCACAGTATCGACCTACTTAGTGCCATGAGAACTGGTGGAGTTGTACCAAATGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTAATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGGTGTAAAGTAGCACCAAATGGCAAAGTCTTACTGACAGAGATTGTTATGTGCTTTGATGATGATGGTGTCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTGGAAGTTTTATTTTTTGA

mRNA sequence

ATGGCAACAACAAAGAGAGATATTGTCTTCTTAGTGTTTGCATTAACTATTTTGTTTCCTATTGTTAAATCACAAACTTTTGATGACTTTTGGTTTGTACAACAATGGCCACCTGCTGTTTGCGCTTCACAATCAGGACGATGTGTAGGAAGAGGGACGCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAAGGAGGAAACTCTGTGATTAATTGCGGTGGCACTCCCTTTGATTTCACTAAGATTGCACATTTAGAAAATGATCTAAACGTAGTTTGGCCAAATGTGGTAACGGGAGACCACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGTTCGGAAAGCAAGTTTGACATGCCAAAGTACTTCCAAACAGCAATAAACATGAGGCACAGTATCGACCTACTTAGTGCCATGAGAACTGGTGGAGTTGTACCAAATGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTAATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGGTGTAAAGTAGCACCAAATGGCAAAGTCTTACTGACAGAGATTGTTATGTGCTTTGATGATGATGGTGTCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTGGAAGTTTTATTTTTTGA

Coding sequence (CDS)

ATGGCAACAACAAAGAGAGATATTGTCTTCTTAGTGTTTGCATTAACTATTTTGTTTCCTATTGTTAAATCACAAACTTTTGATGACTTTTGGTTTGTACAACAATGGCCACCTGCTGTTTGCGCTTCACAATCAGGACGATGTGTAGGAAGAGGGACGCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAAGGAGGAAACTCTGTGATTAATTGCGGTGGCACTCCCTTTGATTTCACTAAGATTGCACATTTAGAAAATGATCTAAACGTAGTTTGGCCAAATGTGGTAACGGGAGACCACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGTTCGGAAAGCAAGTTTGACATGCCAAAGTACTTCCAAACAGCAATAAACATGAGGCACAGTATCGACCTACTTAGTGCCATGAGAACTGGTGGAGTTGTACCAAATGGAGCCTCAAAGGCAAAGCAAAGAGTAGAAAGCGTAATCTCATCCCATTTTGGTAGGGATCCAATTCTTCGGTGTAAAGTAGCACCAAATGGCAAAGTCTTACTGACAGAGATTGTTATGTGCTTTGATGATGATGGTGTCACCCTTATACACTGCAACAAAGCAAGATCCAATTGTGCTGGAAGTTTTATTTTTTGA

Protein sequence

MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLWPQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF

Homology

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BLAST of HG10019722 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 413.7 bits (1062), Expect = 9.8e-112
Identity = 188/216 (87.04%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHG 60
           MATTKR+IV LVF LTILFPIV+SQTFDDFWFVQQWPPAVCA QSGRCVGRGTRSFTIHG
Sbjct: 1   MATTKRNIVLLVFGLTILFPIVQSQTFDDFWFVQQWPPAVCALQSGRCVGRGTRSFTIHG 60

Query: 61  LWPQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESK 120
           LWPQKGG+SV NC G  FDFTKIAHLENDLN+VWPNV+TG++KFFWG+EWNKHGICSESK
Sbjct: 61  LWPQKGGSSVTNCAGNSFDFTKIAHLENDLNIVWPNVITGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 180
           FD   YFQTAINMRHSIDLLS MRTGGV+PNGASKAKQRVESVISSHFG+DPILRCK A 
Sbjct: 121 FDQAAYFQTAINMRHSIDLLSVMRTGGVIPNGASKAKQRVESVISSHFGKDPILRCKTAS 180

Query: 181 NGKVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           NG+VLLTE+VMCFDDDGVTL++CNKARSNCA SFIF
Sbjct: 181 NGQVLLTEVVMCFDDDGVTLLNCNKARSNCATSFIF 216

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BLAST of HG10019722 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 403.7 bits (1036), Expect = 1.0e-108
Identity = 183/216 (84.72%), Postives = 197/216 (91.20%), Query Frame = 0


Query: 1   MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHG 60
           M  TKRD+VF VF LTILFPIVKSQTFD+FWFVQQWPPA+C  QSGRCVGRGTRSFTIHG
Sbjct: 1   MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHG 60

Query: 61  LWPQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESK 120
           LWPQKGG SV NC G  FDFTKIAHLE+DLNVVWPNVVTG++KFFWG+EWNKHGICSESK
Sbjct: 61  LWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 180
           FD  KYFQTAINMRHSIDLLS +RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCK A 
Sbjct: 121 FDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTAS 180

Query: 181 NGKVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           NG+VLLTEIV+CFDDDGVTLI+CNKARSNCA SFIF
Sbjct: 181 NGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

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BLAST of HG10019722 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 402.5 bits (1033), Expect = 2.3e-108
Identity = 185/214 (86.45%), Postives = 194/214 (90.65%), Query Frame = 0


Query: 3   TTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLW 62
           T   D+VF VF LTILFPIVKSQTFDDFWFVQQWPPAVC  QSGRCVGRGTRSFTIHGLW
Sbjct: 4   TKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLW 63

Query: 63  PQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFD 122
           PQKGG SV NC G  FDFTKIAHLENDLNVVWPNVVTG++KFFWG+EWNKHGICSESKFD
Sbjct: 64  PQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFD 123

Query: 123 MPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNG 182
             KYFQTAINMRH IDLLS +RTGGV PNGASKAKQRVE+ ISSHFG+DPILRCK A NG
Sbjct: 124 EAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNG 183

Query: 183 KVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           +VLLTEIVMCFDDDGVTLI+CNKARSNCAGSFIF
Sbjct: 184 QVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

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BLAST of HG10019722 vs. NCBI nr
Match: XP_022934270.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 6.4e-95
Identity = 159/208 (76.44%), Postives = 180/208 (86.54%), Query Frame = 0


Query: 9   VFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLWPQKGGN 68
           +FL+FA  IL PI KSQ FDDFWFVQQWPP VCA QSGRCVGRGT+SFTIHGLWPQK GN
Sbjct: 8   IFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGN 67

Query: 69  SVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFDMPKYFQ 128
           SV NC GT FDF +IAHLENDLNVVWPN++TG+HK+FWG+EWN HGICSES+FD   YFQ
Sbjct: 68  SVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQ 127

Query: 129 TAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKVLLTE 188
           T+IN+RH+IDLLSA+R  GVVPNGASK+KQRVE+ I  HF  +P+LRCK A NG+VLLTE
Sbjct: 128 TSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTE 187

Query: 189 IVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           IVMC DDDGVTLI+CN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

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BLAST of HG10019722 vs. NCBI nr
Match: XP_022983351.1 (ribonuclease MC-like [Cucurbita maxima])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 160/214 (74.77%), Postives = 180/214 (84.11%), Query Frame = 0


Query: 3   TTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLW 62
           TT    +FL+FA  IL PI KSQ FDDFWFVQQWPP VCA Q GRCVGRGT SFTIHGLW
Sbjct: 2   TTPNTQIFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLW 61

Query: 63  PQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFD 122
           PQK GNSV NC GT FDF +IAHLENDLNVVWPN++TG+HK+FWG+EWN HGICSES+FD
Sbjct: 62  PQKRGNSVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFD 121

Query: 123 MPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNG 182
              YFQT+IN+RH+IDLLSA+R  GVVPNGASK+KQRVE+ I  HF  +P+LRCK A NG
Sbjct: 122 EAAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNG 181

Query: 183 KVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           +VLLTEIVMC DDDGVTLI+CN A+SNCA SFIF
Sbjct: 182 QVLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

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BLAST of HG10019722 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 7.2e-57
Identity = 98/193 (50.78%), Postives = 132/193 (68.39%), Query Frame = 0


Query: 27  FDDFWFVQQWPPAVCASQ-SGRCVGRGTRSFTIHGLWPQKGGNSVINC-GGTPFDFTKIA 86
           FD FWFVQQWPPAVC+ Q SG C G G R+FTIHGLWPQ  G S+ NC  G+PFD TKI+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 87  HLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFDMPKYFQTAINMRHSIDLLSAMR 146
           HL++ LN +WPNV+  +++ FW +EW KHG CSES F+   YF+ A++MR++ D++ A+R
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 147 TGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKV-LLTEIVMCFDDDGVTLIHC 206
                PNG +K++Q ++  + + FG+ P LRC+  P  KV  L ++V CF  DG TLI C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 207 NKARSNCAGSFIF 217
              R  C  +FIF
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of HG10019722 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 73/203 (35.96%), Postives = 104/203 (51.23%), Query Frame = 0


Query: 8   IVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCV---GRGTRSFTIHGLWPQ 67
           I  LV   ++      S+ FD F+FVQQWP + C +Q   C    G+    F IHGLWP 
Sbjct: 11  ISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPN 70

Query: 68  -KGGNSVINCGGT-PFDFTKIAHLENDLNVVWPNVV--TGDHKFFWGYEWNKHGICSESK 127
            K G    NC  + PFD + I+ L   +   WP +   +G  + FW +EW KHG CSES 
Sbjct: 71  YKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESV 130

Query: 128 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 187
            D  +YFQTA+N++   +LL A+   G+ P+G S + + +   I    G  P + C    
Sbjct: 131 IDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDG 190

Query: 188 NGKVLLTEIVMCFDDDGVTLIHC 204
           +G   L ++ +C D  G  LI C
Sbjct: 191 SGNSQLYQVYLCVDRSGSGLIEC 213

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BLAST of HG10019722 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 4.9e-29
Identity = 69/213 (32.39%), Postives = 112/213 (52.58%), Query Frame = 0


Query: 1   MATTKRDIVFLVFAL--TILFPIVKSQTFDDFWFVQQWPPAVCASQSGRC---VGRGTRS 60
           MA+     +FL+  +    L  +  ++ FD F+FVQQWP + C ++   C    G+    
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAAD 60

Query: 61  FTIHGLWPQKG-GNSVINCG-GTPFDFTKIAHLENDLNVVWPNVV--TGDHKFFWGYEWN 120
           F IHGLWP    G    NC   +P+D ++I+ L + +   WP +   +G    FW +EW 
Sbjct: 61  FGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWE 120

Query: 121 KHGICSESKF-DMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGR 180
           KHG C+ES   +   YF+ A+++++ IDLLS ++   + P+G S     + + I S  G 
Sbjct: 121 KHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGY 180

Query: 181 DPILRCKVAPNGKVLLTEIVMCFDDDGVTLIHC 204
            P ++C V  +G   L ++ +C D  G +LI C
Sbjct: 181 TPWIQCNVDQSGNSQLYQVYICVDGSGSSLIEC 213

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BLAST of HG10019722 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-26
Identity = 67/203 (33.00%), Postives = 99/203 (48.77%), Query Frame = 0


Query: 10  FLVFALTILFPIVKS--QTFDDFWFVQQWPPAVCASQSGRC---VGRGTRSFTIHGLWPQ 69
           F +F L +    V+S  Q FD F+FV QWP A C S+   C    G+    F IHGLWP 
Sbjct: 3   FFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPN 62

Query: 70  -KGGNSVINCG-GTPFDFTKIAHLENDLNVVWPNV--VTGDHKFFWGYEWNKHGICSESK 129
            K G    NC   + FD  +++ L +DL   WP +   + D   FW +EW KHG C+ES+
Sbjct: 63  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 122

Query: 130 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 189
            D   YF+  + ++   +LL A+   G+ P+      + +E+ I    G  P + C    
Sbjct: 123 LDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDS 182

Query: 190 NGKVLLTEIVMCFDDDGVTLIHC 204
           +    L +I +C D      I+C
Sbjct: 183 SHNSQLYQIYLCVDTSASKFINC 205

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BLAST of HG10019722 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 65/211 (30.81%), Postives = 102/211 (48.34%), Query Frame = 0


Query: 1   MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRC---VGRGTRSFT 60
           M + K+ ++ ++    +L   V SQ FD F+FVQQWP + C ++   C    G+    F+
Sbjct: 2   MKSQKKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFS 61

Query: 61  IHGLWPQ-KGGNSVINCG-GTPFDFTKIAHLENDLNVVWPNVV--TGDHKFFWGYEWNKH 120
           IHGLWP  K G    NC   +  D ++ + L + +   WP++   + D   FW +EW KH
Sbjct: 62  IHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKH 121

Query: 121 GICSESKFDMPKYFQTAINMRHSIDLLSAMRTGGVVP-NGASKAKQRVESVISSHFGRDP 180
           G C  S  +   YFQTA++ +   +LL  +   G+ P NG     + ++  I    G  P
Sbjct: 122 GTC--SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTP 181

Query: 181 ILRCKVAPNGKVLLTEIVMCFDDDGVTLIHC 204
            + C V   G   L ++ +C D      I C
Sbjct: 182 FIECNVDSQGNHQLYQVYLCVDSSASKFIDC 210

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BLAST of HG10019722 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 4.9e-109
Identity = 183/216 (84.72%), Postives = 197/216 (91.20%), Query Frame = 0


Query: 1   MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHG 60
           M  TKRD+VF VF LTILFPIVKSQTFD+FWFVQQWPPA+C  QSGRCVGRGTRSFTIHG
Sbjct: 1   MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHG 60

Query: 61  LWPQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESK 120
           LWPQKGG SV NC G  FDFTKIAHLE+DLNVVWPNVVTG++KFFWG+EWNKHGICSESK
Sbjct: 61  LWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 180
           FD  KYFQTAINMRHSIDLLS +RTGG+VPNGASKAKQRVE+ ISSHF +DPILRCK A 
Sbjct: 121 FDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTAS 180

Query: 181 NGKVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           NG+VLLTEIV+CFDDDGVTLI+CNKARSNCA SFIF
Sbjct: 181 NGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of HG10019722 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.1e-108
Identity = 185/214 (86.45%), Postives = 194/214 (90.65%), Query Frame = 0


Query: 3   TTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLW 62
           T   D+VF VF LTILFPIVKSQTFDDFWFVQQWPPAVC  QSGRCVGRGTRSFTIHGLW
Sbjct: 4   TKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLW 63

Query: 63  PQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFD 122
           PQKGG SV NC G  FDFTKIAHLENDLNVVWPNVVTG++KFFWG+EWNKHGICSESKFD
Sbjct: 64  PQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFD 123

Query: 123 MPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNG 182
             KYFQTAINMRH IDLLS +RTGGV PNGASKAKQRVE+ ISSHFG+DPILRCK A NG
Sbjct: 124 EAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNG 183

Query: 183 KVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           +VLLTEIVMCFDDDGVTLI+CNKARSNCAGSFIF
Sbjct: 184 QVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of HG10019722 vs. ExPASy TrEMBL
Match: A0A6J1F776 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.1e-95
Identity = 159/208 (76.44%), Postives = 180/208 (86.54%), Query Frame = 0


Query: 9   VFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLWPQKGGN 68
           +FL+FA  IL PI KSQ FDDFWFVQQWPP VCA QSGRCVGRGT+SFTIHGLWPQK GN
Sbjct: 8   IFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGN 67

Query: 69  SVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFDMPKYFQ 128
           SV NC GT FDF +IAHLENDLNVVWPN++TG+HK+FWG+EWN HGICSES+FD   YFQ
Sbjct: 68  SVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQ 127

Query: 129 TAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKVLLTE 188
           T+IN+RH+IDLLSA+R  GVVPNGASK+KQRVE+ I  HF  +P+LRCK A NG+VLLTE
Sbjct: 128 TSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTE 187

Query: 189 IVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           IVMC DDDGVTLI+CN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of HG10019722 vs. ExPASy TrEMBL
Match: A0A6J1J1Y7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 5.3e-95
Identity = 160/214 (74.77%), Postives = 180/214 (84.11%), Query Frame = 0


Query: 3   TTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHGLW 62
           TT    +FL+FA  IL PI KSQ FDDFWFVQQWPP VCA Q GRCVGRGT SFTIHGLW
Sbjct: 2   TTPNTQIFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLW 61

Query: 63  PQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESKFD 122
           PQK GNSV NC GT FDF +IAHLENDLNVVWPN++TG+HK+FWG+EWN HGICSES+FD
Sbjct: 62  PQKRGNSVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFD 121

Query: 123 MPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNG 182
              YFQT+IN+RH+IDLLSA+R  GVVPNGASK+KQRVE+ I  HF  +P+LRCK A NG
Sbjct: 122 EAAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNG 181

Query: 183 KVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           +VLLTEIVMC DDDGVTLI+CN A+SNCA SFIF
Sbjct: 182 QVLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of HG10019722 vs. ExPASy TrEMBL
Match: A0A6J1FBD8 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.6e-94
Identity = 160/216 (74.07%), Postives = 182/216 (84.26%), Query Frame = 0


Query: 1   MATTKRDIVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCVGRGTRSFTIHG 60
           MAT    I FL+F    L P+ KSQ FDDFWFVQQWPP VCA QSGRCVGRGT SFTIHG
Sbjct: 1   MATPNTQI-FLLFVFVSLLPMAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIHG 60

Query: 61  LWPQKGGNSVINCGGTPFDFTKIAHLENDLNVVWPNVVTGDHKFFWGYEWNKHGICSESK 120
           LWPQKGGNSV NC GT FDF +I+HLENDLN+VWPN++TG+HK+FWG+EWN HGICSESK
Sbjct: 61  LWPQKGGNSVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESK 120

Query: 121 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 180
           FD   YFQT+IN+RH+I+LLSA+R  GVVPNGASK+KQRVES I SHF + P+LRCK A 
Sbjct: 121 FDQKAYFQTSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTAS 180

Query: 181 NGKVLLTEIVMCFDDDGVTLIHCNKARSNCAGSFIF 217
           NG+VLLTEIVMC DDDGVTLI+CN A+SNCA +FIF
Sbjct: 181 NGQVLLTEIVMCIDDDGVTLINCNIAKSNCANNFIF 215

[Close]

BLAST of HG10019722 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-33
Identity = 73/203 (35.96%), Postives = 104/203 (51.23%), Query Frame = 0


Query: 8   IVFLVFALTILFPIVKSQTFDDFWFVQQWPPAVCASQSGRCV---GRGTRSFTIHGLWPQ 67
           I  LV   ++      S+ FD F+FVQQWP + C +Q   C    G+    F IHGLWP 
Sbjct: 11  ISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPN 70

Query: 68  -KGGNSVINCGGT-PFDFTKIAHLENDLNVVWPNVV--TGDHKFFWGYEWNKHGICSESK 127
            K G    NC  + PFD + I+ L   +   WP +   +G  + FW +EW KHG CSES 
Sbjct: 71  YKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESV 130

Query: 128 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 187
            D  +YFQTA+N++   +LL A+   G+ P+G S + + +   I    G  P + C    
Sbjct: 131 IDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDG 190

Query: 188 NGKVLLTEIVMCFDDDGVTLIHC 204
           +G   L ++ +C D  G  LI C
Sbjct: 191 SGNSQLYQVYLCVDRSGSGLIEC 213

[Close]

BLAST of HG10019722 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-28
Identity = 63/193 (32.64%), Postives = 93/193 (48.19%), Query Frame = 0


Query: 18  LFPIVKSQTFDDFWFVQQWPPAVCASQSGRCV---GRGTRSFTIHGLWPQ-KGGNSVINC 77
           LF     + FD F+FV QWP A C +    C    G+    F IHGLWP   GG+   NC
Sbjct: 19  LFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNC 78

Query: 78  G-GTPFDFTKIAHLENDLNVVWPNVVTGDHK--FFWGYEWNKHGICSESKFDMPKYFQTA 137
              + FD ++I+ L + L   WP +    ++   FW +EW KHG CSES  D  +YF+ A
Sbjct: 79  DPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENA 138

Query: 138 INMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKVLLTEIV 197
           + ++   +LL  ++  G+ P+       ++ + I    G  P + C   P     L +I 
Sbjct: 139 LKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIY 198

Query: 198 MCFDDDGVTLIHC 204
           +C D  G   I C
Sbjct: 199 ICVDTSGTEFIEC 211

[Close]

BLAST of HG10019722 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 121.3 bits (303), Expect = 9.4e-28
Identity = 67/203 (33.00%), Postives = 99/203 (48.77%), Query Frame = 0


Query: 10  FLVFALTILFPIVKS--QTFDDFWFVQQWPPAVCASQSGRC---VGRGTRSFTIHGLWPQ 69
           F +F L +    V+S  Q FD F+FV QWP A C S+   C    G+    F IHGLWP 
Sbjct: 3   FFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPN 62

Query: 70  -KGGNSVINCG-GTPFDFTKIAHLENDLNVVWPNV--VTGDHKFFWGYEWNKHGICSESK 129
            K G    NC   + FD  +++ L +DL   WP +   + D   FW +EW KHG C+ES+
Sbjct: 63  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 122

Query: 130 FDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAP 189
            D   YF+  + ++   +LL A+   G+ P+      + +E+ I    G  P + C    
Sbjct: 123 LDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDS 182

Query: 190 NGKVLLTEIVMCFDDDGVTLIHC 204
           +    L +I +C D      I+C
Sbjct: 183 SHNSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of HG10019722 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.6e-25
Identity = 66/207 (31.88%), Postives = 96/207 (46.38%), Query Frame = 0


Query: 11  LVFALTILFPIV--KSQT---FDDFWFVQQWPPAVCASQSGRC---VGRGTRSFTIHGLW 70
           ++ +L IL  +V   SQT   F+ F++V  WP A+C SQ G C    G     F IHGLW
Sbjct: 5   IIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLW 64

Query: 71  PQ-KGGNSVINCGGTP-FDFTKIAHLENDLNVVWPN----VVTGDHKFFWGYEWNKHGIC 130
           PQ   G     C  T  FD +KI+ L   +   W          +    W +EWNKHG C
Sbjct: 65  PQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTC 124

Query: 131 SESKFDMPKYFQTAINMRHSIDLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRC 190
            +S FD   YF+T +  +H + LL+ +   G+ PN    +   +++ I    G  P + C
Sbjct: 125 VQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIEC 184

Query: 191 KVAPNGKVLLTEIVMCFDDDGVTLIHC 204
                G   L +I +C D+     + C
Sbjct: 185 NEDVKGNKQLFQIYICLDNYAKEFVEC 211

[Close]

BLAST of HG10019722 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 79.0 bits (193), Expect = 5.4e-15
Identity = 59/189 (31.22%), Postives = 83/189 (43.92%), Query Frame = 0


Query: 27  FDDFWFVQQWPPAV------CASQSGRCVGR-GTRSFTIHGLWPQ-KGGNSVINCGGTPF 86
           FD F    QWP         C S++  C G      FTIHGLWP    G+    C  + F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 87  DFTKIAHLENDLNVVWPNV-------VTGDHKFFWGYEWNKHGICSESKF-DMPKYFQTA 146
              +I+ L + L   WP++         G    FWG+EW KHG CS   F D   YF T 
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 147 IN--MRHSI-DLLSAMRTGGVVPNGASKAKQRVESVISSHFGRDPILRCKVAPNGKVLLT 197
           +N  ++H++ D+L   + G V  N        + + I + F   P + CK     +  + 
Sbjct: 152 LNLYLKHNVTDVL--YQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCK-----RDAID 211

The following BLAST results are available for this feature:

BLAST of HG10019722 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905534.1	9.8e-112	87.04	ribonuclease MC-like [Benincasa hispida]	[more]
XP_008443402.1	1.0e-108	84.72	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
XP_004147499.1	2.3e-108	86.45	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_022934270.1	6.4e-95	76.44	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_022983351.1	1.1e-94	74.77	ribonuclease MC-like [Cucurbita maxima]	[more]

BLAST of HG10019722 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	7.2e-57	50.78	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.1e-32	35.96	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	4.9e-29	32.39	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	1.3e-26	33.00	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80196	1.5e-25	30.81	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of HG10019722 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8Q3	4.9e-109	84.72	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A0A0LC37	1.1e-108	86.45	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1F776	3.1e-95	76.44	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1	[more]
A0A6J1J1Y7	5.3e-95	74.77	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1	[more]
A0A6J1FBD8	2.6e-94	74.07	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1	[more]

BLAST of HG10019722 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.5e-33	35.96	ribonuclease 1	[more]
AT1G14220.1	2.5e-28	32.64	Ribonuclease T2 family protein	[more]
AT1G26820.1	9.4e-28	33.00	ribonuclease 3	[more]
AT1G14210.1	2.6e-25	31.88	Ribonuclease T2 family protein	[more]
AT2G39780.1	5.4e-15	31.22	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 28..203 e-value: 2.1E-40 score: 138.8
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 16..211
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 27..214 e-value: 7.5E-52 score: 178.1
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 27..216
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 16..211
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 56..63
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 105..116
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 27..215 e-value: 1.55543E-67 score: 204.103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019722.1	HG10019722.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding