HG10019350 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019350 vs. NCBI nr
Match: XP_016900385.1 (PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo] >XP_016900386.1 PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 544/659 (82.55%), Postives = 562/659 (85.28%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS YEDLSTSWDLFSVS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDI+FIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. NCBI nr
Match: XP_011651533.1 (long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739343.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739344.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739345.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >XP_031739346.1 long chain acyl-CoA synthetase 1 [Cucumis sativus] >KGN58128.1 hypothetical protein Csa_017503 [Cucumis sativus])

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 545/659 (82.70%), Postives = 559/659 (84.83%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEGREGKDGKPSVGPVYRNVLAE +FPS YEDLSTSWDLFSVS  KYAER+M
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSANKYAERRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKAKELLNS  TIPERLKV ICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSGCTIPERLKVFICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNEL LEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELCLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. NCBI nr
Match: XP_038903045.1 (LOW QUALITY PROTEIN: long chain acyl-CoA synthetase 1-like [Benincasa hispida])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 538/665 (80.90%), Postives = 558/665 (83.91%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEGREGKDGKPSV PVYRNVLAENDFPS YED ST+WDLFSVS KKYAER+M
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVEPVYRNVLAENDFPSPYEDSSTTWDLFSVSAKKYAERRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEV  DVLNIGSALRAIG EP S IGIYGA  PQWIIAME 
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVXGDVLNIGSALRAIGAEPVSVIGIYGAKLPQWIIAMEG 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKE------LLNSETTIPERLK 180
           CNAHSLI +PLYDTLGHGAVNFIIDHAEIDIVF+Q+K+ K       LLNSE+ IPERLK
Sbjct: 121 CNAHSLIXLPLYDTLGHGAVNFIIDHAEIDIVFVQDKEGKRSKSCCLLLNSESAIPERLK 180

Query: 181 VVICFTSLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGT 240
           VVICFTSLKEEEKI AAGNG+KPYSWDEFL +GKENP E+TPPRPL+ICTIMYTSGTSGT
Sbjct: 181 VVICFTSLKEEEKISAAGNGLKPYSWDEFLRMGKENPFEVTPPRPLNICTIMYTSGTSGT 240

Query: 241 PKGVVLTQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASV 300
           PKGVVLTQET+T FIRGVDIYM+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASV
Sbjct: 241 PKGVVLTQETLTMFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASV 300

Query: 301 GYYHG------------------------------------------------------- 360
           GYYHG                                                       
Sbjct: 301 GYYHGDLNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQVLNPVRRKAFDLLYKYKLL 360

Query: 361 ----------------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQG 420
                                 VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF+QG
Sbjct: 361 WMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFIQG 420

Query: 421 YGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKT 480
           YGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNP GDRPCGEICVRGKT
Sbjct: 421 YGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPLGDRPCGEICVRGKT 480

Query: 481 VFTEYYKDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLEN 540
           VFTEYYK+PELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLEN
Sbjct: 481 VFTEYYKNPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLEN 540

Query: 541 VYSITPIIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL 583
           VYS+TPI+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL
Sbjct: 541 VYSVTPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVL 600

BLAST of HG10019350 vs. NCBI nr
Match: TYK20490.1 (long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.3e-307
Identity = 533/659 (80.88%), Postives = 550/659 (83.46%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS             VS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLCKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. NCBI nr
Match: KAA0038117.1 (long chain acyl-CoA synthetase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 533/668 (79.79%), Postives = 550/668 (82.34%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS             VS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGTKALLIICRIKQAVQVLNPVRRKAFDLLCKY 360

Query: 361 -------------------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420
                                    VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF
Sbjct: 361 KLSWMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420

Query: 421 VQGYGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVR 480
           VQGYGLTETCGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVR
Sbjct: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480

Query: 481 GKTVFTEYYKDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEY 540
           GKTVFTEYYKDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEY
Sbjct: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEY 540

Query: 541 LENVYSITPIIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 583
           LENVYSITPI+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH
Sbjct: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600

BLAST of HG10019350 vs. ExPASy Swiss-Prot
Match: O22898 (Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.2e-229
Identity = 377/658 (57.29%), Postives = 474/658 (72.04%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+E  FP +  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA G EPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+Q+ K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FLH+G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           T + + TF+ G+D+YMDQFEDKMT +DVYLSFLPLAHILDR+ EEYFFRKGASVGYYHG 
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           ++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLGTVG   ++NE+RLEEV EMGY+P G+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 582
           +++DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

BLAST of HG10019350 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.8e-166
Identity = 289/657 (43.99%), Postives = 402/657 (61.19%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 61
           K++  +VEEG+EG DG+PSVGPVYR++ A++ FP   E + + WD+F +S +KY    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 121
           G R+ V+GKPG Y+W+TY+EVY  V+ +G++LR++GV+  ++ GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVF++EKK  EL  +     E +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL LG+    ++   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 301
            E+I T I GV   +    + +T  DVYLS+LPLAHI DRV+EE F + GA++G++ G  
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
                          VK  LGG +R+I+SG A L++ VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 481
             T +  PDE+ MLGTVG  +   ++RLE VPEM Y+       GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 582
           ++ +W+YGNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

BLAST of HG10019350 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.7e-166
Identity = 294/658 (44.68%), Postives = 395/658 (60.03%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 61
           KRF  +VE  +E  DG PSVGPVYR+  A+N FP+  + + + WD+F  + +KY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 121
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G++ G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I F++EKK  EL  +     + +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL LG+    E+   +P DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 301
            E+I T   GV  ++      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ G  
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
                          VK  LGG +R+I+SG A L++ +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 481
             T   FPDE+ MLGTVG  +   ++RLE VPEM Y+  G  P GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 583
           IE IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

BLAST of HG10019350 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.4e-162
Identity = 288/652 (44.17%), Postives = 397/652 (60.89%), Query Frame = 0

Query: 3   RFSVKVEEGREGKD-GKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 62
           R+ V+VE+G++G D G PSVGPVYR++ A++ FP   +DL ++WD+F +S +K     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 63  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 122
           G R+ V+GK G Y+W+TYKEV++ V+ +G+++R IGV  G + GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 123 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 182
           NAH L CVPLYDTLG GA+ FII HAE+ + F +E K  ELL +     + LK ++ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 183 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 242
           +   +++ A  + +  YSWD+FL LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 243 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 302
            E+I   + GV   +   ++++T  DVYLS+LPLAHI DRV+EE    + AS+G++ G  
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 363 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 422
                          VK  LGG +RLI+SG A L+  +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 423 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 482
           G T +  P+E+ MLGTVG  +   ++RLE VPEMGY+     P GEIC+RGKT+F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 483 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 542
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 543 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 577
           IE IW+YGNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604

BLAST of HG10019350 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.7e-148
Identity = 272/655 (41.53%), Postives = 371/655 (56.64%), Query Frame = 0

Query: 4   FSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQMLGW 63
           ++VKVEE R   +  PS GPVYR++ A +    L E L + W+  S S K+  +  MLG 
Sbjct: 3   YTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLGR 62

Query: 64  RKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEACNA 123
           R+  + + GPY+W TY+EV+ + + + SA+R+ GV PG R GIYG NCPQWI+AM+AC +
Sbjct: 63  RQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQACYS 122

Query: 124 HSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTSLK 183
           H++  VPLYDTLG  AV FII+H E+ I F+QE K   +L+        LK ++ F ++ 
Sbjct: 123 HAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGNIT 182

Query: 184 EEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLTQE 243
             +K      G+  +SW+EF  LG  +  E+      DICT+MYTSG +G PKGV+LT E
Sbjct: 183 SVQKKETEALGVSCFSWEEFSQLGNLS-GELPEKHRTDICTLMYTSGATGEPKGVILTNE 242

Query: 244 TITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG---- 303
            I   I   D  ++  +   +E DVY S+LPLAH+ D++VE Y   KG+++GY+ G    
Sbjct: 243 AIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDIRF 302

Query: 304 ------------------------------------------------------------ 363
                                                                       
Sbjct: 303 LMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLPHG 362

Query: 364 -------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETCGP 423
                        +K   GGR+RL+ SG A L   VEE+LRVT CA   QGYGLTE+CG 
Sbjct: 363 QAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESCGG 422

Query: 424 TTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYKDP 483
                 +   M+GTVG  M   E RLE VPEMGY+   D+P GEIC+RG T+F+ Y+K  
Sbjct: 423 CLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHKRE 482

Query: 484 ELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPIIE 543
           +LTK+ + DGWFHTGDIGE  PNG +KIIDRKKN+ KLSQGEY+ +E +E +Y   P+I 
Sbjct: 483 DLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPLIA 542

Query: 544 DIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTAER 582
            IW+YGNSF+S LVAVVV      + WA +      F  LC   +   Y+L EL STA++
Sbjct: 543 SIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTAKK 602

BLAST of HG10019350 vs. ExPASy TrEMBL
Match: A0A1S4DWM9 (long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 544/659 (82.55%), Postives = 562/659 (85.28%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS YEDLSTSWDLFSVS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDI+FIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. ExPASy TrEMBL
Match: A0A0A0L8J2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G535120 PE=4 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 545/659 (82.70%), Postives = 559/659 (84.83%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEGREGKDGKPSVGPVYRNVLAE +FPS YEDLSTSWDLFSVS  KYAER+M
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSANKYAERRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKAKELLNS  TIPERLKV ICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSGCTIPERLKVFICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNEL LEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELCLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. ExPASy TrEMBL
Match: A0A5D3DAJ7 (Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00640 PE=4 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.2e-308
Identity = 533/659 (80.88%), Postives = 550/659 (83.46%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS             VS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLCKYKLSWMNSGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVRGKTVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 583
           I+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

BLAST of HG10019350 vs. ExPASy TrEMBL
Match: A0A5A7T933 (Long chain acyl-CoA synthetase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003380 PE=4 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 6.9e-307
Identity = 533/668 (79.79%), Postives = 550/668 (82.34%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFSVKVEEG+EGKDG+PSVGPVYRNVLAE +FPS             VS KKYA+R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPS------------PVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRAIG EPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQ+KKA ELLNSE  IP+RLKVVICFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEEEKI A  NGMKPYSWDEFL LGKENPSEI+PPRPLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           TQETITTFIRGVDI M+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGTKALLIICRIKQAVQVLNPVRRKAFDLLCKY 360

Query: 361 -------------------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420
                                    VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF
Sbjct: 361 KLSWMNSGYKQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFF 420

Query: 421 VQGYGLTETCGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVR 480
           VQGYGLTETCGPTTIGFPDEMCMLG VGGV LFNELRLEEVP+MGYNP GDRPCGEICVR
Sbjct: 421 VQGYGLTETCGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVR 480

Query: 481 GKTVFTEYYKDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEY 540
           GKTVFTEYYKDPELTKE+IKDGWFHTGDIGE+FPNGV+KIIDRKKNLIKLSQGEYIALEY
Sbjct: 481 GKTVFTEYYKDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEY 540

Query: 541 LENVYSITPIIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 583
           LENVYSITPI+EDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH
Sbjct: 541 LENVYSITPIVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRH 600

BLAST of HG10019350 vs. ExPASy TrEMBL
Match: A0A6J1F2G0 (long chain acyl-CoA synthetase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441583 PE=4 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 9.3e-304
Identity = 521/658 (79.18%), Postives = 550/658 (83.59%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MKRFS+KVEEGREGKDGKPS+GPVYRNV+A++  PSL+EDL+TSWDLF VS +KYA RQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSIGPVYRNVIAKDGSPSLFEDLNTSWDLFRVSAEKYAGRQM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFV+GKPGPYIWKTYKEVY DVLNIGSALRA+GV+PGSRIGIYG NCPQWIIAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAVGVKPGSRIGIYGENCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           CNAHS+ICVPLYDTLGH AVNFIIDHAEIDIVF+Q+KKAKELL SE T PERLKVV+CFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           SLKEE+KI AA NG+K YSWDEF  LGKENPSEITPPR LDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEDKISAADNGVKTYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           T ET+ TFIRGVDIYM+QFEDKMTE+DVYLSFLPLAHILDR VEEYFFRKGASVGYYHG 
Sbjct: 241 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNGIKEDLMELKPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYRYKLSWMNRGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           VKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
           CGPTTIGFPDEMCMLGTVG V  FN+LRLEEVPEMGYNP GDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELTKEAIKDGWFHTGDIGEI PNGVVKIIDRKKNLIKLSQGEYIALEYLENVYS TP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 582
           I+EDIWIYGNSFKS LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR+YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

BLAST of HG10019350 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 795.4 bits (2053), Expect = 3.0e-230
Identity = 377/658 (57.29%), Postives = 474/658 (72.04%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+E  FP +  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA G EPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+Q+ K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FLH+G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           T + + TF+ G+D+YMDQFEDKMT +DVYLSFLPLAHILDR+ EEYFFRKGASVGYYHG 
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           ++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLGTVG   ++NE+RLEEV EMGY+P G+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 582
           +++DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

BLAST of HG10019350 vs. TAIR 10
Match: AT2G47240.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 795.4 bits (2053), Expect = 3.0e-230
Identity = 377/658 (57.29%), Postives = 474/658 (72.04%), Query Frame = 0

Query: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQM 60
           MK F+ KVEEG +G DGKPSVGPVYRN+L+E  FP +  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA G EPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VF+Q+ K K LL  +    +RLK ++ FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVL 240
           ++ +E    A+  G+K YSW +FLH+G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 TQETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG- 300
           T + + TF+ G+D+YMDQFEDKMT +DVYLSFLPLAHILDR+ EEYFFRKGASVGYYHG 
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 ----------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420
                           ++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYY 480
            G T +GFPDEMCMLGTVG   ++NE+RLEEV EMGY+P G+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KDPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELT+E +KDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IIEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 582
           +++DIW+YG+SFKSMLVAVVV + E   +WA   GF   F ELC   +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

BLAST of HG10019350 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-167
Identity = 289/657 (43.99%), Postives = 402/657 (61.19%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 61
           K++  +VEEG+EG DG+PSVGPVYR++ A++ FP   E + + WD+F +S +KY    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 121
           G R+ V+GKPG Y+W+TY+EVY  V+ +G++LR++GV+  ++ GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVF++EKK  EL  +     E +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL LG+    ++   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 301
            E+I T I GV   +    + +T  DVYLS+LPLAHI DRV+EE F + GA++G++ G  
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
                          VK  LGG +R+I+SG A L++ VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 481
             T +  PDE+ MLGTVG  +   ++RLE VPEM Y+       GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 582
           ++ +W+YGNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

BLAST of HG10019350 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 585.5 bits (1508), Expect = 4.7e-167
Identity = 294/658 (44.68%), Postives = 395/658 (60.03%), Query Frame = 0

Query: 2   KRFSVKVEEGREGKDGKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 61
           KRF  +VE  +E  DG PSVGPVYR+  A+N FP+  + + + WD+F  + +KY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 121
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G++ G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I F++EKK  EL  +     + +K V+ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL LG+    E+   +P DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 301
            E+I T   GV  ++      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ G  
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 421
                          VK  LGG +R+I+SG A L++ +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 481
             T   FPDE+ MLGTVG  +   ++RLE VPEM Y+  G  P GEIC+RGKT+F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 583
           IE IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

BLAST of HG10019350 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 573.2 bits (1476), Expect = 2.4e-163
Identity = 288/652 (44.17%), Postives = 397/652 (60.89%), Query Frame = 0

Query: 3   RFSVKVEEGREGKD-GKPSVGPVYRNVLAENDFPSLYEDLSTSWDLFSVSTKKYAERQML 62
           R+ V+VE+G++G D G PSVGPVYR++ A++ FP   +DL ++WD+F +S +K     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 63  GWRKFVEGKPGPYIWKTYKEVYSDVLNIGSALRAIGVEPGSRIGIYGANCPQWIIAMEAC 122
           G R+ V+GK G Y+W+TYKEV++ V+ +G+++R IGV  G + GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 123 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQEKKAKELLNSETTIPERLKVVICFTS 182
           NAH L CVPLYDTLG GA+ FII HAE+ + F +E K  ELL +     + LK ++ F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 183 LKEEEKILAAGNGMKPYSWDEFLHLGKENPSEITPPRPLDICTIMYTSGTSGTPKGVVLT 242
           +   +++ A  + +  YSWD+FL LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 243 QETITTFIRGVDIYMDQFEDKMTENDVYLSFLPLAHILDRVVEEYFFRKGASVGYYHG-- 302
            E+I   + GV   +   ++++T  DVYLS+LPLAHI DRV+EE    + AS+G++ G  
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 363 ---------------VKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTETC 422
                          VK  LGG +RLI+SG A L+  +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 423 GPTTIGFPDEMCMLGTVGGVMLFNELRLEEVPEMGYNPRGDRPCGEICVRGKTVFTEYYK 482
           G T +  P+E+ MLGTVG  +   ++RLE VPEMGY+     P GEIC+RGKT+F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 483 DPELTKEAIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 542
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 543 IEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTSTA 577
           IE IW+YGNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604