HG10016986 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016986
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionsubtilisin-like protease SBT4.15
LocationChr03: 9900030 .. 9904641 (+)
RNA-Seq ExpressionHG10016986
SyntenyHG10016986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCCAAAATCTACAGCTATGGAAAGAGCTTCAATGGGTTCGCCGCAAGGCTTTTGCCTCACGAAGCCACCAAGCTCTCAAGTAATTAATTCAAAATTCATGAATTATTAATATTATTATTATTCGACATGTCGTTTTTAATGGTTTAATTATAAATTTTGGGCAGATGAGGAGGGTGTGGTTTCGGTATTTCAGAGCAGAAAACAGAGAGTTGTGACAACAAGATCCTGGGACTTTTTAGGACTAAATCATCATTATTCCAAACGTAACCCTAAAATTGAGTCTAATCTCATTGTCGCCGTTTTGGATACGGGTCCTTCTCTTCTCTTTCATTAATTTCATTCTAACTAATTCCAATTAAATCTAACCTAATTACTTCTTTATAATAATATTGGATTATAAATCTTAATTTATTTTGTTGTATTAAATGTTTAGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGCGTCACTGGTCCCAACTTCACAGCCTGCAACAAGTATTTTCTTTTTCTTTTTTCTTTTTATCCCTAACTAAACTCATAATATACTCAATGTTTAAAATAATGTGCACAACAAGTATTTTCTTTTTCTTTTTTCTTTTTATCCCTAACTAAACTCATAATATACTCAATGTTTAAAATAATGTGCATTTGTGTGCATGGAATTCTTAAAAGGGTTGTTTTTAAATATAAAAATATGATTCAAATTATTTATAAATATAGAAAAAATTATGTTTATGCCCTCATTCATTAATTTTGTTTTTACCAACCTTACCCCTCAAAATTGGATGACAAATTGTAAAACTGAATTTTTTTTAAGAAGGATGACGAATTGTAAAACTAATTTTTTTTTCCCTACTGTCCCACCCTCAAACATGAAGTAATAAATGGTAGAATCGGTATTTTTACCTACAATGTAAGAAGTTAAAAAAAAAATATTTATTTATTTTTAAAAGAGTAAGGTGCAACCAACTACGTCCATTTTCAATTATCACAAAGAAAAAAAATATATAGTCAATTTGCACTCAAAATATTTTTCTTTTTGAAAATGGTCTTTTATCCAAATAAAAAGAAAAAATTTTACAAATAAAAAATTGTCAAACTATCTCTATAAAATAATAAAAGAAAATACTGATAGACATCGATAAACTTATCATGCTGATAGATCTTGATAGACTTCTACCAGCCTCTATCACAACTATTTAAAAATTTTGCTATTTGGTGTAAATAGTTTACCTTATTTTCTGTTTTTAAAACTTTCCCAAATAAAAACTTAGGACTAATTTTTGTCAATTTACCCTGAGACAGCAAAGTAATCGGTGCGAATTACTTCGATCTGGAAAGCGTAAGCTCATATCCAGAGTCGAGCGTGGCGGACACGGAGGGGCATGGCTCTCACACGGCGTCGACGGTGGCAGGCTCGGCGGTGGAGGGGGCGAGCTTGTATGGTCTGGCCAAAGGGACGGCGCGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAGTATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGATGAGGCCATCGCCGACGGCGTCGATTTCATTTCCGTCTCCATAGGCTCGCCGCCGATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTTCATGCCATGAAGAAGGGAATATTGACGTCCTGCGCCGCCGGCAATGACGGCCCTGAATTGTCCACCGTCGAGAATGTGGCGCCCTGGATTATGACCGTCGCTGCAACGGCCATTGATAGACGGTTCGTCACCGCTTTCAAACTCGGTAATGGCAACAAATTTACTGTGAGTCTCTCTCTCTTTCTCTAATATATTAATGGGGAATTTTTAAGGAGATTTTTACCAAATGACAAACTATTTAAAAAAAAAAAAAATACTGATAGAAGACTTCTATCAGAATCTATCAATTATAGACTTATATTATTTCTATCATTGATAAATTTCTATTACATCTATCACAACTATCTAAAAATTTTGTTTTTATATTTTTAAAAGTCTATTAATGTTTATCATTATTTTTTTTTGCTATTTTGTGTAAATAATTTTTCTTATATTAATTGTGTGTGTTTTTTACTAAATTATACTAAATACTTTTTAAAATTTTAGAGAATTATTATAAAAGAAAAAAATATCAAACTATTTACAAATATAAAAAAATTTCAATGTCTATCAACGATAGACTACGATAGACAGGAAAAAAAAATTTATATTTGTAAATAGTTTTGTCATTTTTCTATATTTGAAAATAATTATTTAAATTTTGTATTTCATGTCAAAATTAAAATTAATAGTGAAAATTTTCTATATTCATAAATAATTTGATATTTTTTCTATTTATAATAATTTTCCTTAAATTTACATAGTTTAAAAAATGTTATTAAACTTTAAAAAACATTATAAATACCCTTTGTAATGCCATTAAACTTTAGAAAAGATATTAAAAATACCTTTTAAAAATAACCTTAATGTTAACACTCATAAAAAAAATACAAATTATTCTTCGAAGTTAATTTTTTGGTTTACTTTTTTTATATTAAACGAATTTACTTAATTGTGTAAGGGAAGTTTGTGTTTACCATTTTTTTTTAAGTAGAATAAAAATAGAGAATTTCAAACTACATATCTTTTAATCGCTAATAAATTTATATGCCAATTAAGTTATACTTATAAAGTCAGCCCATTAATACATACAATTCATGTTGAATAATGGGAGAAAAAAAAATCTAGGTGAAGCTTAGATTTTCCCTAGTGACCATAACTTAATAAAAATTGACTTTGACATGTACTATTCGGTTGAATAGAATTCAAACGATCGAACGAATTTTAGAACACACACAATATTTAATTTTTGAGCATTATATTTAATCACAAAATACATATTAAAGTCTAGACATGAAAGAGTTAAAAAAAAAAAAAAAAAAAAGCTATTATTTTGGATACATGCAAACCTGTATGAATAATTCAAATGTGTTTATTTAAATGAAGTGAAAAGAAAATGAAAATGTTGGTAGGGATTTTCCATCAATACTTTCTCGCCAAAGAAAGAAATGTACTCTCTTACAAGCGGTGCCAAAGCGTCCAACAATGGCGGATCCTATCAAGGAAATGCAAGGTATGAAATATGAATATGATTATTTGAAAAAGGTTGTTTATTTTTCATTAAAGTTGAAATGGCCGGGATGACAGTGCTTGTGATCCAGAAGCTATAAACCAAAGCAAGGTGAAGGGAAAGATTGTATATTGCTTGAAAACTTACACAGATCCCTACATCAAATCCTTAGGAGGCACTGGAGTCATTCAACTCACTCAACAACAAACTGATTATTCCTCCATTTTGCTTCTTCCTGGGGCTTCCATCTCTTCTCTCTCTGGCAAATACATCGATCTTTATATCAACTCCACCAAGTATGTTTCTATCTTTTTGTGAATTCCTGCTCCTTTTCAGTTGTCTCTCTTTTTAATTGTATTGATTCATTGATCACAGGAATCCTAAAGCTATCATTTATAAGAGTGAAACTGTCAAAATTGATGCTCCTTTTGTTGCTTCTTTCTCATCTAGAGGGCCTCAGTTTATAAGTAGTAACATTCTCAAGGTATAACACAATAACAAATGTCTCTTTGATTTCTTTATTCTTAGAAAAAAAAAAAAGGACTAAGTTTCTATGGGTAGCCTGATCTTTCTGCACCAGGGATAGACATCTTGGCTGCTTATACAAAACTAGCAAGTTTGACAGGGGATATGTCAGACAGTAGATATAGTTTATTCACTGTAATGTCAGGTACATCCATGGCCTGTCCTCATGCTACGGCGGCTGCTGCGTACGTTAAGTCATTCCACCCTGACTGGTCTCCGGCTGCGGTCAAGTCTGCTCTCATGACCACTGGTAGAGTCTTTCATGTCACCATATTTCTATCAGCTAATGTTCAAGCCTGACTGGCTGGATTTGGTTTTTCCTGGTAAATTTTAGCAACTCCAATGAAGATGAAATCCAAGGATGCTGTGCTTGGCTCTGGAGCAGGACAAATAAATCCAACCAAGGCAGTGCATCCCGGCCTTGTTTACAATATCTCGTTCAACTCCTACATCTCTTTCCTCTGCAAAGAAGGCTACAACAGCACAACAATTGGCCTACTTGATGGTAACAAGAAGTATAACTGCTCCAAGATCAAACCTGCACAAGGAACCGATGGACTCAACTACCCGACAATGCACAAACAGCTCTCAGATCCTAGTTCTGCCATCAAGGCGGTCTTTTATCGAACAGTGACTCATGTCGGGTATGGTGCATCGTTGTATAGGGCAAATATATCATCCCCAGATGGAGTATCTGTCAAAGTTTTTCCAGACACCCTGAATTTTGCTAAGTTACATGAAACGAAGACGTTTAAAGTTGTGGTGAAGGGCAAACCTATGCCAGAAGGAACACGGATTCTATCAGCTTTACTTGAATGGAACGACTCTAAACACATAGTTAGAAGCAATATCCTTATCTACAGGCAATTGATCTAA

mRNA sequence

ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCCAAAATCTACAGCTATGGAAAGAGCTTCAATGGGTTCGCCGCAAGGCTTTTGCCTCACGAAGCCACCAAGCTCTCAAATGAGGAGGGTGTGGTTTCGGTATTTCAGAGCAGAAAACAGAGAGTTGTGACAACAAGATCCTGGGACTTTTTAGGACTAAATCATCATTATTCCAAACGTAACCCTAAAATTGAGTCTAATCTCATTGTCGCCGTTTTGGATACGGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGCGTCACTGGTCCCAACTTCACAGCCTGCAACAACAAAGTAATCGGTGCGAATTACTTCGATCTGGAAAGCGTAAGCTCATATCCAGAGTCGAGCGTGGCGGACACGGAGGGGCATGGCTCTCACACGGCGTCGACGGTGGCAGGCTCGGCGGTGGAGGGGGCGAGCTTGTATGGTCTGGCCAAAGGGACGGCGCGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAGTATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGATGAGGCCATCGCCGACGGCGTCGATTTCATTTCCGTCTCCATAGGCTCGCCGCCGATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTTCATGCCATGAAGAAGGGAATATTGACGTCCTGCGCCGCCGGCAATGACGGCCCTGAATTGTCCACCGTCGAGAATGTGGCGCCCTGGATTATGACCGTCGCTGCAACGGCCATTGATAGACGGTTCGTCACCGCTTTCAAACTCGGTAATGGCAACAAATTTACTGGATTTTCCATCAATACTTTCTCGCCAAAGAAAGAAATGTACTCTCTTACAAGCGGTGCCAAAGCGTCCAACAATGGCGGATCCTATCAAGGAAATGCAAGTGCTTGTGATCCAGAAGCTATAAACCAAAGCAAGGTGAAGGGAAAGATTGTATATTGCTTGAAAACTTACACAGATCCCTACATCAAATCCTTAGGAGGCACTGGAGTCATTCAACTCACTCAACAACAAACTGATTATTCCTCCATTTTGCTTCTTCCTGGGGCTTCCATCTCTTCTCTCTCTGGCAAATACATCGATCTTTATATCAACTCCACCAAGAATCCTAAAGCTATCATTTATAAGAGTGAAACTGTCAAAATTGATGCTCCTTTTGTTGCTTCTTTCTCATCTAGAGGGCCTCAGTTTATAAGTAGTAACATTCTCAAGCCTGATCTTTCTGCACCAGGGATAGACATCTTGGCTGCTTATACAAAACTAGCAAGTTTGACAGGGGATATGTCAGACAGTAGATATAGTTTATTCACTGTAATGTCAGGTACATCCATGGCCTGTCCTCATGCTACGGCGGCTGCTGCGTACGTTAAGTCATTCCACCCTGACTGGTCTCCGGCTGCGGTCAAGTCTGCTCTCATGACCACTGCAACTCCAATGAAGATGAAATCCAAGGATGCTGTGCTTGGCTCTGGAGCAGGACAAATAAATCCAACCAAGGCAGTGCATCCCGGCCTTGTTTACAATATCTCGTTCAACTCCTACATCTCTTTCCTCTGCAAAGAAGGCTACAACAGCACAACAATTGGCCTACTTGATGGTAACAAGAAGTATAACTGCTCCAAGATCAAACCTGCACAAGGAACCGATGGACTCAACTACCCGACAATGCACAAACAGCTCTCAGATCCTAGTTCTGCCATCAAGGCGGTCTTTTATCGAACAGTGACTCATGTCGGGTATGGTGCATCGTTGTATAGGGCAAATATATCATCCCCAGATGGAGTATCTGTCAAAGTTTTTCCAGACACCCTGAATTTTGCTAAGTTACATGAAACGAAGACGTTTAAAGTTGTGGTGAAGGGCAAACCTATGCCAGAAGGAACACGGATTCTATCAGCTTTACTTGAATGGAACGACTCTAAACACATAGTTAGAAGCAATATCCTTATCTACAGGCAATTGATCTAA

Coding sequence (CDS)

ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCCAAAATCTACAGCTATGGAAAGAGCTTCAATGGGTTCGCCGCAAGGCTTTTGCCTCACGAAGCCACCAAGCTCTCAAATGAGGAGGGTGTGGTTTCGGTATTTCAGAGCAGAAAACAGAGAGTTGTGACAACAAGATCCTGGGACTTTTTAGGACTAAATCATCATTATTCCAAACGTAACCCTAAAATTGAGTCTAATCTCATTGTCGCCGTTTTGGATACGGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGCGTCACTGGTCCCAACTTCACAGCCTGCAACAACAAAGTAATCGGTGCGAATTACTTCGATCTGGAAAGCGTAAGCTCATATCCAGAGTCGAGCGTGGCGGACACGGAGGGGCATGGCTCTCACACGGCGTCGACGGTGGCAGGCTCGGCGGTGGAGGGGGCGAGCTTGTATGGTCTGGCCAAAGGGACGGCGCGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAGTATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGATGAGGCCATCGCCGACGGCGTCGATTTCATTTCCGTCTCCATAGGCTCGCCGCCGATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTTCATGCCATGAAGAAGGGAATATTGACGTCCTGCGCCGCCGGCAATGACGGCCCTGAATTGTCCACCGTCGAGAATGTGGCGCCCTGGATTATGACCGTCGCTGCAACGGCCATTGATAGACGGTTCGTCACCGCTTTCAAACTCGGTAATGGCAACAAATTTACTGGATTTTCCATCAATACTTTCTCGCCAAAGAAAGAAATGTACTCTCTTACAAGCGGTGCCAAAGCGTCCAACAATGGCGGATCCTATCAAGGAAATGCAAGTGCTTGTGATCCAGAAGCTATAAACCAAAGCAAGGTGAAGGGAAAGATTGTATATTGCTTGAAAACTTACACAGATCCCTACATCAAATCCTTAGGAGGCACTGGAGTCATTCAACTCACTCAACAACAAACTGATTATTCCTCCATTTTGCTTCTTCCTGGGGCTTCCATCTCTTCTCTCTCTGGCAAATACATCGATCTTTATATCAACTCCACCAAGAATCCTAAAGCTATCATTTATAAGAGTGAAACTGTCAAAATTGATGCTCCTTTTGTTGCTTCTTTCTCATCTAGAGGGCCTCAGTTTATAAGTAGTAACATTCTCAAGCCTGATCTTTCTGCACCAGGGATAGACATCTTGGCTGCTTATACAAAACTAGCAAGTTTGACAGGGGATATGTCAGACAGTAGATATAGTTTATTCACTGTAATGTCAGGTACATCCATGGCCTGTCCTCATGCTACGGCGGCTGCTGCGTACGTTAAGTCATTCCACCCTGACTGGTCTCCGGCTGCGGTCAAGTCTGCTCTCATGACCACTGCAACTCCAATGAAGATGAAATCCAAGGATGCTGTGCTTGGCTCTGGAGCAGGACAAATAAATCCAACCAAGGCAGTGCATCCCGGCCTTGTTTACAATATCTCGTTCAACTCCTACATCTCTTTCCTCTGCAAAGAAGGCTACAACAGCACAACAATTGGCCTACTTGATGGTAACAAGAAGTATAACTGCTCCAAGATCAAACCTGCACAAGGAACCGATGGACTCAACTACCCGACAATGCACAAACAGCTCTCAGATCCTAGTTCTGCCATCAAGGCGGTCTTTTATCGAACAGTGACTCATGTCGGGTATGGTGCATCGTTGTATAGGGCAAATATATCATCCCCAGATGGAGTATCTGTCAAAGTTTTTCCAGACACCCTGAATTTTGCTAAGTTACATGAAACGAAGACGTTTAAAGTTGTGGTGAAGGGCAAACCTATGCCAGAAGGAACACGGATTCTATCAGCTTTACTTGAATGGAACGACTCTAAACACATAGTTAGAAGCAATATCCTTATCTACAGGCAATTGATCTAA

Protein sequence

MIGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILSALLEWNDSKHIVRSNILIYRQLI
Homology
[Close]
BLAST of HG10016986 vs. NCBI nr
Match: XP_038882509.1 (subtilisin-like protease SBT4.15 [Benincasa hispida])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 643/682 (94.28%), Postives = 661/682 (96.92%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           IG EKIARES+IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVF+SRKQRVVTTRSWDFL
Sbjct: 61  IGDEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 120

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 121 GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGA+YFDL++VS YPESSVADTEGHGSHTASTVAGS VEGASLYGL KGTARGGVPSA
Sbjct: 181 KVIGASYFDLDNVSQYPESSVADTEGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSA 240

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCW+IFCSEMDVLAGF++AIADGVD ISVSIGS PMDFFRDGQAIGAFHAMKKG
Sbjct: 241 RIAVYKVCWNIFCSEMDVLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKG 300

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK
Sbjct: 301 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 360

Query: 302 EMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGVI 361
           +MYSLTSGAKASNNG  YQGNASACDPEAI+QSKVKGKIVYCLKTYTDPYIKSLGGTGVI
Sbjct: 361 QMYSLTSGAKASNNGTPYQGNASACDPEAISQSKVKGKIVYCLKTYTDPYIKSLGGTGVI 420

Query: 362 QLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFSS 421
           QLTQQQ+DYSSILLLPG +I S+SGK IDLYINSTKNPKA+IYKS TVK+DAPFVASFSS
Sbjct: 421 QLTQQQSDYSSILLLPGVAIPSVSGKLIDLYINSTKNPKAVIYKSRTVKVDAPFVASFSS 480

Query: 422 RGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATAA 481
           RGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYS FTV SGTSMACPHATAA
Sbjct: 481 RGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSFFTVKSGTSMACPHATAA 540

Query: 482 AAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISFN 541
           AAYVKSFHPDWSPAAVKSALMTTATPMK+KS+D VLGSGAGQINPTKAVHPGLVYNISFN
Sbjct: 541 AAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDVVLGSGAGQINPTKAVHPGLVYNISFN 600

Query: 542 SYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVFY 601
           SYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDP SAIKAVFY
Sbjct: 601 SYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPRSAIKAVFY 660

Query: 602 RTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILS 661
           RTVTHVGYGASLYRANISSPDG+SVKVFPDTLNF KLHETKTFKVVVKGKPMPEGT   S
Sbjct: 661 RTVTHVGYGASLYRANISSPDGLSVKVFPDTLNFVKLHETKTFKVVVKGKPMPEGTWTQS 720

Query: 662 ALLEWNDSKHIVRSNILIYRQL 684
           ALLEWNDSKHIVRSNILIYRQL
Sbjct: 721 ALLEWNDSKHIVRSNILIYRQL 742

[Close]
BLAST of HG10016986 vs. NCBI nr
Match: XP_016902874.1 (PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo])

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 625/684 (91.37%), Postives = 651/684 (95.18%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           +GSEKIARESKIYSYGKSFNGFAARLLPHEATK+SNEE VVSVF+SRK+RVVTTRSWDFL
Sbjct: 1   LGSEKIARESKIYSYGKSFNGFAARLLPHEATKISNEESVVSVFESRKKRVVTTRSWDFL 60

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNP IESNLI+AV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 61  GLNHRYSKRNPLIESNLIIAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 120

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGANYFDL  VSSYPE SVADTEGHGSHTASTVAGSAV GASLYGLAKGTARGGVPSA
Sbjct: 121 KVIGANYFDLAKVSSYPELSVADTEGHGSHTASTVAGSAVAGASLYGLAKGTARGGVPSA 180

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWSIFC+EMDVLA FDEAIADGVD ISVSIGSP MDFFRDGQAIGAFHAMKKG
Sbjct: 181 RIAVYKVCWSIFCNEMDVLAAFDEAIADGVDLISVSIGSPAMDFFRDGQAIGAFHAMKKG 240

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILT+ AAGNDGP+LSTVENVAPWIMTVAATAIDR FVT+F+LGNGNK TG SINTFSP+K
Sbjct: 241 ILTTAAAGNDGPQLSTVENVAPWIMTVAATAIDRGFVTSFELGNGNKLTGGSINTFSPQK 300

Query: 302 EMYSLTSGAK-ASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +MYSLTSGAK ASNN   +QGNASACDP AINQSKVKGKIVYCLKTYTDP IKSLGGTGV
Sbjct: 301 QMYSLTSGAKAASNNATPHQGNASACDPNAINQSKVKGKIVYCLKTYTDPSIKSLGGTGV 360

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQLT QQTDYSSILLLPGA+I S+SGKYIDLYINSTKNPKA+IYKSETVKIDAPFVASFS
Sbjct: 361 IQLTTQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 420

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTGD SDSRYS FTVMSGTSMACPHATA
Sbjct: 421 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACPHATA 480

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+KSKDA+LGSGAGQINPTKAVHPGLVYNISF
Sbjct: 481 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSKDAMLGSGAGQINPTKAVHPGLVYNISF 540

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           NSYISFLCKEGYNSTTIGLL G+KKYNC+KIKPAQGTDGLNYPTMHKQLS PSS I+AVF
Sbjct: 541 NSYISFLCKEGYNSTTIGLLGGSKKYNCTKIKPAQGTDGLNYPTMHKQLSGPSSTIEAVF 600

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVTHVGYGASLYRANISSP  +SVKVFPDTLNF KLHETKTFKVVVKGKPMP+GT+IL
Sbjct: 601 YRTVTHVGYGASLYRANISSPASLSVKVFPDTLNFVKLHETKTFKVVVKGKPMPKGTQIL 660

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIVRSNILIYR+LI
Sbjct: 661 SALLEWTDSKHIVRSNILIYRKLI 684

[Close]
BLAST of HG10016986 vs. NCBI nr
Match: XP_031743407.1 (subtilisin-like protease SBT4.15 isoform X1 [Cucumis sativus])

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 618/684 (90.35%), Postives = 651/684 (95.18%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           IG EKIARESKIYSYGKSFNGFAARLLP EATKLS+EE VVSVF+SRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNP IESNLIVAV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGANYFDL+ V+SYPE SVADT+GHGSH ASTVAGSAV GASLYGLAKGTARGGVPSA
Sbjct: 181 KVIGANYFDLDKVTSYPELSVADTDGHGSHIASTVAGSAVAGASLYGLAKGTARGGVPSA 240

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWS+FC+EMDVLA FDEAIADGVD ISVSIGSPPMDFFRDGQAIGAFHAMKKG
Sbjct: 241 RIAVYKVCWSVFCNEMDVLAAFDEAIADGVDLISVSIGSPPMDFFRDGQAIGAFHAMKKG 300

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILT+ AAGNDGPEL TVENVAPWIMTVAAT IDR FVTAF+LGNGNKFTG SINTFSP+K
Sbjct: 301 ILTTTAAGNDGPELFTVENVAPWIMTVAATGIDRGFVTAFELGNGNKFTGGSINTFSPQK 360

Query: 302 EMYSLTSGAKAS-NNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +M+SLTSGAKA+ NNG  +QGNASACDP A+NQSKVKGKIVYCLKTYTDP IKSLGGTGV
Sbjct: 361 QMHSLTSGAKAAFNNGTPHQGNASACDPNAMNQSKVKGKIVYCLKTYTDPSIKSLGGTGV 420

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQLTQQQTDYSSILLLPGA+I S+SGKYIDLYINSTKNPKA+IYKSETVKIDAPFVASFS
Sbjct: 421 IQLTQQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 480

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTGD SDSRYS FTVMSGTSMAC HATA
Sbjct: 481 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACSHATA 540

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+KS+D VLGSGAGQINPTKAVHPGLVYNISF
Sbjct: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDVVLGSGAGQINPTKAVHPGLVYNISF 600

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           +SYISFLCKEGYN+TTIGLL G+KKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAI+AVF
Sbjct: 601 DSYISFLCKEGYNNTTIGLLGGSKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIEAVF 660

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVTHVGYGASLYRANISSPD +SVKVFPDTLNF KLHET+TFKVVVKGKPMP+GT+IL
Sbjct: 661 YRTVTHVGYGASLYRANISSPDSLSVKVFPDTLNFVKLHETRTFKVVVKGKPMPKGTQIL 720

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIVRSNILIYR+ I
Sbjct: 721 SALLEWTDSKHIVRSNILIYREKI 744

[Close]
BLAST of HG10016986 vs. NCBI nr
Match: XP_031743408.1 (subtilisin-like protease SBT4.15 isoform X2 [Cucumis sativus])

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 610/684 (89.18%), Postives = 642/684 (93.86%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           IG EKIARESKIYSYGKSFNGFAARLLP EATKLS+EE VVSVF+SRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNP IESNLIVAV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGANYFDL+ V+SYPE SVADT+GHGSH ASTVAGSAV GASLYGLAKGTARGGVPSA
Sbjct: 181 KVIGANYFDLDKVTSYPELSVADTDGHGSHIASTVAGSAVAGASLYGLAKGTARGGVPSA 240

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWS+FC+EMDVLA FDEAIADGVD ISVSIGSPPMDFFRDGQAIGAFHAMKKG
Sbjct: 241 RIAVYKVCWSVFCNEMDVLAAFDEAIADGVDLISVSIGSPPMDFFRDGQAIGAFHAMKKG 300

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILT+ AAGNDGPEL TVENVAPWIMTVAAT IDR FVTAF+LGNGNKFT          K
Sbjct: 301 ILTTTAAGNDGPELFTVENVAPWIMTVAATGIDRGFVTAFELGNGNKFT----------K 360

Query: 302 EMYSLTSGAKAS-NNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +M+SLTSGAKA+ NNG  +QGNASACDP A+NQSKVKGKIVYCLKTYTDP IKSLGGTGV
Sbjct: 361 QMHSLTSGAKAAFNNGTPHQGNASACDPNAMNQSKVKGKIVYCLKTYTDPSIKSLGGTGV 420

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQLTQQQTDYSSILLLPGA+I S+SGKYIDLYINSTKNPKA+IYKSETVKIDAPFVASFS
Sbjct: 421 IQLTQQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 480

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTGD SDSRYS FTVMSGTSMAC HATA
Sbjct: 481 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACSHATA 540

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+KS+D VLGSGAGQINPTKAVHPGLVYNISF
Sbjct: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDVVLGSGAGQINPTKAVHPGLVYNISF 600

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           +SYISFLCKEGYN+TTIGLL G+KKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAI+AVF
Sbjct: 601 DSYISFLCKEGYNNTTIGLLGGSKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIEAVF 660

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVTHVGYGASLYRANISSPD +SVKVFPDTLNF KLHET+TFKVVVKGKPMP+GT+IL
Sbjct: 661 YRTVTHVGYGASLYRANISSPDSLSVKVFPDTLNFVKLHETRTFKVVVKGKPMPKGTQIL 720

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIVRSNILIYR+ I
Sbjct: 721 SALLEWTDSKHIVRSNILIYREKI 734

[Close]
BLAST of HG10016986 vs. NCBI nr
Match: XP_031743409.1 (subtilisin-like protease SBT4.15 isoform X3 [Cucumis sativus])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 586/649 (90.29%), Postives = 618/649 (95.22%), Query Frame = 0 

Query: 37  NEEGVVSVFQSRKQRVVTTRSWDFLGLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSD 96
           NEE VVSVF+SRK+RV+TTRSW+FLGLNH YSKRNP IESNLIVAV DTGIWIDSPSFSD
Sbjct: 23  NEESVVSVFESRKKRVLTTRSWEFLGLNHQYSKRNPLIESNLIVAVFDTGIWIDSPSFSD 82

Query: 97  EGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDLESVSSYPESSVADTEGHGSHTASTV 156
           EGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDL+ V+SYPE SVADT+GHGSH ASTV
Sbjct: 83  EGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDLDKVTSYPELSVADTDGHGSHIASTV 142

Query: 157 AGSAVEGASLYGLAKGTARGGVPSARIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISV 216
           AGSAV GASLYGLAKGTARGGVPSARIAVYKVCWS+FC+EMDVLA FDEAIADGVD ISV
Sbjct: 143 AGSAVAGASLYGLAKGTARGGVPSARIAVYKVCWSVFCNEMDVLAAFDEAIADGVDLISV 202

Query: 217 SIGSPPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRR 276
           SIGSPPMDFFRDGQAIGAFHAMKKGILT+ AAGNDGPEL TVENVAPWIMTVAAT IDR 
Sbjct: 203 SIGSPPMDFFRDGQAIGAFHAMKKGILTTTAAGNDGPELFTVENVAPWIMTVAATGIDRG 262

Query: 277 FVTAFKLGNGNKFTGFSINTFSPKKEMYSLTSGAKAS-NNGGSYQGNASACDPEAINQSK 336
           FVTAF+LGNGNKFTG SINTFSP+K+M+SLTSGAKA+ NNG  +QGNASACDP A+NQSK
Sbjct: 263 FVTAFELGNGNKFTGGSINTFSPQKQMHSLTSGAKAAFNNGTPHQGNASACDPNAMNQSK 322

Query: 337 VKGKIVYCLKTYTDPYIKSLGGTGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINS 396
           VKGKIVYCLKTYTDP IKSLGGTGVIQLTQQQTDYSSILLLPGA+I S+SGKYIDLYINS
Sbjct: 323 VKGKIVYCLKTYTDPSIKSLGGTGVIQLTQQQTDYSSILLLPGATIPSVSGKYIDLYINS 382

Query: 397 TKNPKAIIYKSETVKIDAPFVASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTG 456
           TKNPKA+IYKSETVKIDAPFVASFSSRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTG
Sbjct: 383 TKNPKAVIYKSETVKIDAPFVASFSSRGPQRISSNILKPDLSAPGIDILAAYTKLATLTG 442

Query: 457 DMSDSRYSLFTVMSGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDA 516
           D SDSRYS FTVMSGTSMAC HATAAAAYVKSFHPDWSPAAVKSALMTTATPMK+KS+D 
Sbjct: 443 DTSDSRYSFFTVMSGTSMACSHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDV 502

Query: 517 VLGSGAGQINPTKAVHPGLVYNISFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQ 576
           VLGSGAGQINPTKAVHPGLVYNISF+SYISFLCKEGYN+TTIGLL G+KKYNCSKIKPAQ
Sbjct: 503 VLGSGAGQINPTKAVHPGLVYNISFDSYISFLCKEGYNNTTIGLLGGSKKYNCSKIKPAQ 562

Query: 577 GTDGLNYPTMHKQLSDPSSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNF 636
           GTDGLNYPTMHKQLSDPSSAI+AVFYRTVTHVGYGASLYRANISSPD +SVKVFPDTLNF
Sbjct: 563 GTDGLNYPTMHKQLSDPSSAIEAVFYRTVTHVGYGASLYRANISSPDSLSVKVFPDTLNF 622

Query: 637 AKLHETKTFKVVVKGKPMPEGTRILSALLEWNDSKHIVRSNILIYRQLI 685
            KLHET+TFKVVVKGKPMP+GT+ILSALLEW DSKHIVRSNILIYR+ I
Sbjct: 623 VKLHETRTFKVVVKGKPMPKGTQILSALLEWTDSKHIVRSNILIYREKI 671

[Close]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 2.2e-229
Identity = 405/701 (57.77%), Postives = 520/701 (74.18%), Query Frame = 0 

Query: 1   MIGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDF 60
           +IG E  ARE KIYSYGK+ NGF ARL PHEA KLS EEGVVSVF++ ++++ TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 61  LGLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LGL     KR+  IESN+IV VLDTGI ++SPSF+D+G GPPP KWKGKCVTG NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 121 NKVIGANYFDLESVSSYPE---SSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGG 180
           NKVIGA YF ++S    P+    + AD +GHG+HT+ST+AG +V  ASL+G+A GTARGG
Sbjct: 181 NKVIGAKYFHIQS-EGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGG 240

Query: 181 VPSARIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHA 240
           VPSARIA YKVCW   C++MD+LA FDEAI+DGVD IS+SIG   + FF D  AIGAFHA
Sbjct: 241 VPSARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHA 300

Query: 241 MKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTF 300
           MK+GILT+C+AGN+GP L TV N+APW+MTVAA ++DR+F T  KLGNG   +G S+N F
Sbjct: 301 MKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGF 360

Query: 301 SPKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLK---------TYT 360
           +P+K+MY LTSG+ ASN      G  S C+P  + + KV GK+VYC              
Sbjct: 361 NPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQ 420

Query: 361 DPYIKSLGGTGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSET 420
           D  ++SL G GVI    + TD ++  L+ G+ +    G  I  YINSTKNP+A+I+K++T
Sbjct: 421 DHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKT 480

Query: 421 VKIDAPFVASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVM 480
            K+ AP ++SFS+RGPQ IS NILKPD+SAPG++ILAAY+KLAS+TG   D+R +LF++M
Sbjct: 481 TKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIM 540

Query: 481 SGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTK 540
           SGTSMACPHA AAAAYVKSFHPDWSPAA+KSALMTTATPM++K  +A L  G+GQINP +
Sbjct: 541 SGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRR 600

Query: 541 AVHPGLVYNISFNSYISFLCKEGYNSTTIGLLDGN-------KKYNCSKIKPAQGTDGLN 600
           A+HPGLVY+I+ ++Y+ FLCKEGYNST+IGLL G+       K+YNC  IK   G+DGLN
Sbjct: 601 AIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLN 660

Query: 601 YPTMHKQLSDPSSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHET 660
           YP++HKQ++   + +  VFYRTVT+VGYG S Y A + +P G+ V+V P  ++F +  E 
Sbjct: 661 YPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEK 720

Query: 661 KTFKVVVKGKPMPEGTRILSALLEWNDSK-HIVRSNILIYR 682
           + FKVV+ G        I+SA +EW+DS+ H+VRS IL++R
Sbjct: 721 RNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFR 760

[Close]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 2.1e-187
Identity = 350/682 (51.32%), Postives = 463/682 (67.89%), Query Frame = 0 

Query: 4   SEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGL 63
           S++ A+E K+YSY K+FN FAA+L PHEA K+   E VVSV +++ +++ TT+SWDF+GL
Sbjct: 66  SQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGL 125

Query: 64  NHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKV 123
               +KR+ K E ++I+ VLDTGI  DS SF D G GPPP KWKG C    NFT CNNK+
Sbjct: 126 -PLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKI 185

Query: 124 IGANYFDLE-SVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSAR 183
           IGA YF  + +V +    S  D +GHG+HT+STVAG  V  ASLYG+A GTARG VPSAR
Sbjct: 186 IGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSAR 245

Query: 184 IAVYKVCWS-IFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 243
           +A+YKVCW+   C++MD+LAGF+ AI DGV+ IS+SIG P  D+  D  ++G+FHAM+KG
Sbjct: 246 LAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKG 305

Query: 244 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 303
           ILT  +AGNDGP   TV N  PWI+TVAA+ IDR F +   LGNG  F+G  I+ FSPK 
Sbjct: 306 ILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKA 365

Query: 304 EMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLK--TYTDPYIKSLGGTG 363
           + Y L SG  A+ N    +  A  C  +++++ KVKGK++ C       +  IKS GG G
Sbjct: 366 KSYPLVSGVDAAKNTDD-KYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAG 425

Query: 364 VIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASF 423
            I ++ Q  D + I + P  S++S  G  I  YINST++  A+I K+  V I APFVASF
Sbjct: 426 AIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASF 485

Query: 424 SSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHAT 483
           SSRGP   S  +LKPD++APGIDILAA+T   SLTG   D+++S FT++SGTSMACPH  
Sbjct: 486 SSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVA 545

Query: 484 AAAAYVKSFHPDWSPAAVKSALMTTATPMKMK-SKDAVLGSGAGQINPTKAVHPGLVYNI 543
             AAYVKSFHPDW+PAA+KSA++T+A P+  + +KDA    G GQINP +A  PGLVY++
Sbjct: 546 GVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDM 605

Query: 544 SFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKA 603
              SY+ FLC EGYN+TT+  L G +  +CS I P  G D LNYPT+   L    ++  A
Sbjct: 606 DDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLA 665

Query: 604 VFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTR 663
           VF R VT+VG  +S+Y A + +P GV + V P +L+F+K  + ++FKVVVK K M  G +
Sbjct: 666 VFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPG-K 725

Query: 664 ILSALLEWNDSKHIVRSNILIY 681
           I+S LL W   +H VRS I+IY
Sbjct: 726 IVSGLLVWKSPRHSVRSPIVIY 744

[Close]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.8e-157
Identity = 312/684 (45.61%), Postives = 434/684 (63.45%), Query Frame = 0 

Query: 1   MIGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDF 60
           ++GS   A ES +++Y +SFNGFA +L   EA K+++ EGVVSVF +    + TTRSWDF
Sbjct: 59  VVGS-TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDF 118

Query: 61  LGLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LG      +R+ ++ESN++V VLDTGIW +SPSF DEG+ PPPPKWKG C T  NF  CN
Sbjct: 119 LGFPLTVPRRS-QVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNF-RCN 178

Query: 121 NKVIGANYFDL-ESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVP 180
            K+IGA  + +   +S    +   DT GHG+HTAST AG  V  A+LYGL  GTARGGVP
Sbjct: 179 RKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVP 238

Query: 181 SARIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSI-GSPPMDFFRDGQAIGAFHAM 240
            ARIA YKVCW+  CS+ D+LA +D+AIADGVD IS+S+ G+ P  +F D  AIG+FHA+
Sbjct: 239 LARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAV 298

Query: 241 KKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFS 300
           ++GILTS +AGN GP   T  +++PW+++VAA+ +DR+FVT  ++GNG  F G SINTF 
Sbjct: 299 ERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD 358

Query: 301 PKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTY-TDPYIKSLGG 360
              + Y L SG    N G   +  +  C  +++N + +KGKIV C  ++    + KSL G
Sbjct: 359 --NQYYPLVSGRDIPNTGFD-KSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDG 418

Query: 361 TGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETV-KIDAPFV 420
              + +T    DY+    LP + +          YI S ++P A I+KS T+    AP V
Sbjct: 419 AAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVV 478

Query: 421 ASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACP 480
            SFSSRGP   + +++KPD+S PG++ILAA+  +A + G     R +LF ++SGTSM+CP
Sbjct: 479 VSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGG---IRRNTLFNIISGTSMSCP 538

Query: 481 HATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMK-SKDAVLGSGAGQINPTKAVHPGLV 540
           H T  A YVK+++P WSPAA+KSALMTTA+PM  + +  A    G+G +NP KAV PGLV
Sbjct: 539 HITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLV 598

Query: 541 YNISFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSA 600
           Y+ + + Y+ FLC +GYN+  +  + G+    C+     +  D LNYP+    +S PS  
Sbjct: 599 YDANESDYVKFLCGQGYNTQAVRRITGDYS-ACTSGNTGRVWD-LNYPSFGLSVS-PSQT 658

Query: 601 IKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPE 660
               F RT+T V   AS YRA IS+P G+++ V P+ L+F  L + K+F + V+G     
Sbjct: 659 FNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIK-- 718

Query: 661 GTRILSALLEWNDSKHIVRSNILI 680
              ++SA L W+D  H VRS I I
Sbjct: 719 -GFVVSASLVWSDGVHYVRSPITI 727

[Close]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 6.7e-157
Identity = 316/679 (46.54%), Postives = 433/679 (63.77%), Query Frame = 0 

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGLNHHYSKRNP 72
           + SY +SFNGFAA L   E+ KL N + VVSVF S+   + TTRSWDF+G      + + 
Sbjct: 69  VRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESV 128

Query: 73  KIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDLE 132
           K ES++IV V+D+GIW +S SF DEG+GPPP KWKG C  G  F ACNNK+IGA +++  
Sbjct: 129 K-ESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYNKF 188

Query: 133 SVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARIAVYKVCWSI 192
           +       S  D EGHG+HTAST AG+AV+ AS YGLA+GTARGGVPSARIA YKVC++ 
Sbjct: 189 A------DSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN- 248

Query: 193 FCSEMDVLAGFDEAIADGVDFISVSIGSPPM-DFFRDGQAIGAFHAMKKGILTSCAAGND 252
            C+++D+LA FD+AIADGVD IS+SI +  + +      AIG+FHAM +GI+T+ +AGN+
Sbjct: 249 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 308

Query: 253 GPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEMYSLTSGAK 312
           GP+  +V NV+PW++TVAA+  DR+F+    LGNG   TG S+NTF+     + +  G  
Sbjct: 309 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQN 368

Query: 313 ASNNGGSYQGNASACDPEAINQSKVKGKIVYC--LKTYTDPYIKSLGGTGVIQLTQQQTD 372
            S N    Q  A  C    ++   VKGKIV C     Y + Y+   G  GVI       D
Sbjct: 369 VSRN--CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLPD 428

Query: 373 YSSILLLPGASISSLSGKYIDLYINSTKNPKA-IIYKSETVKIDAPFVASFSSRGPQFIS 432
            + ++  P +S+     K I  YI S + P+A I+   E V  +AP+V SFSSRGP F+ 
Sbjct: 429 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 488

Query: 433 SNILKPDLSAPGIDILAAYTKLASLTGDMS--DSRYSLFTVMSGTSMACPHATAAAAYVK 492
            N+LKPD+SAPG++ILAA++ +AS +  ++  D R   ++VMSGTSMACPH    AAYVK
Sbjct: 489 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 548

Query: 493 SFHPDWSPAAVKSALMTTATPMKM-KSKDAVLGSGAGQINPTKAVHPGLVYNISFNSYIS 552
           SFHPDWSP+A+KSA+MTTATPM + K+ +     G+GQINPTKA  PGLVY +    Y+ 
Sbjct: 549 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 608

Query: 553 FLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLS--DPSSAIKAVFYRT 612
            LC EG++STT+    G +   CS+    +    LNYPTM   +S  DP       F RT
Sbjct: 609 MLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTMTTFVSSLDP---FNVTFKRT 668

Query: 613 VTHVGYGASLYRANI--SSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILS 672
           VT+VG+  S Y+A++    P+ + + + P+ L F  L E K+F V + GK + +G+ + S
Sbjct: 669 VTNVGFPNSTYKASVVPLQPE-LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSS 725

Query: 673 ALLEWNDSKHIVRSNILIY 681
           +++ W+D  H VRS I+ Y
Sbjct: 729 SVV-WSDGSHSVRSPIVAY 725

[Close]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 3.0e-149
Identity = 307/688 (44.62%), Postives = 424/688 (61.63%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           +  E   ++  + +Y +SFNGFAARL   E   L++ + VVSVF S+   + TT SW+F+
Sbjct: 60  VTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFM 119

Query: 62  GLNH-HYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 121
           GL     +KRNP IES+ I+ V+D+GI+ +S SFS +G+GPPP KWKG C  G NFT CN
Sbjct: 120 GLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CN 179

Query: 122 NKVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPS 181
           NK+IGA Y+    +  +PES+  D  GHGSHTAS  AG+AV+  S YGL  GT RGGVP+
Sbjct: 180 NKLIGARYY-TPKLEGFPESA-RDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPA 239

Query: 182 ARIAVYKVC--WSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFF-RDGQAIGAFHA 241
           ARIAVYKVC    I C+   +LA FD+AIAD VD I+VS+G+  +  F  D  AIGAFHA
Sbjct: 240 ARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHA 299

Query: 242 MKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTF 301
           M KGILT   AGN+GPE  T+ ++APW+ TVAA+ ++R F+T   LGNG    G S+N+F
Sbjct: 300 MAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSF 359

Query: 302 SPKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGG 361
               + Y L  G  AS+   +   +A  C P  ++  +VKGKIV C         +++G 
Sbjct: 360 DLNGKKYPLVYGKSASSRCDA--SSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGA 419

Query: 362 TGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETV-KIDAPFV 421
              I +     D +S+   P + +S      +  Y+NSTKNPKA + KSET+    AP V
Sbjct: 420 VASI-VRNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVV 479

Query: 422 ASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACP 481
           AS+SSRGP  +  +ILKPD++APG +ILAAY+    +    SD+R+  +TV+SGTSM+CP
Sbjct: 480 ASYSSRGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCP 539

Query: 482 HATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKD----AVLGSGAGQINPTKAVHP 541
           H    AAY+K+FHP WSP+ ++SA+MTTA PM   +      A    GAG ++P  A+HP
Sbjct: 540 HVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHP 599

Query: 542 GLVYNISFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDP 601
           GLVY  + + +I+FLC   Y    + L+ G+   +C+K +    T  LNYP+M  Q+S  
Sbjct: 600 GLVYEANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSG- 659

Query: 602 SSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKP 661
           +   K  F RTVT+VG   + Y+A +     + VKV P  L+   L+E K+F V V G  
Sbjct: 660 TKPFKVTFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAG 719

Query: 662 MPEGTRILSALLEWNDSKHIVRSNILIY 681
            P+   ++SA L W+D  H VRS I++Y
Sbjct: 720 -PKAENLVSAQLIWSDGVHFVRSPIVVY 735

[Close]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match: A0A1S4E3S0 (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=3 SV=1)

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 625/684 (91.37%), Postives = 651/684 (95.18%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           +GSEKIARESKIYSYGKSFNGFAARLLPHEATK+SNEE VVSVF+SRK+RVVTTRSWDFL
Sbjct: 1   LGSEKIARESKIYSYGKSFNGFAARLLPHEATKISNEESVVSVFESRKKRVVTTRSWDFL 60

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNP IESNLI+AV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 61  GLNHRYSKRNPLIESNLIIAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 120

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGANYFDL  VSSYPE SVADTEGHGSHTASTVAGSAV GASLYGLAKGTARGGVPSA
Sbjct: 121 KVIGANYFDLAKVSSYPELSVADTEGHGSHTASTVAGSAVAGASLYGLAKGTARGGVPSA 180

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWSIFC+EMDVLA FDEAIADGVD ISVSIGSP MDFFRDGQAIGAFHAMKKG
Sbjct: 181 RIAVYKVCWSIFCNEMDVLAAFDEAIADGVDLISVSIGSPAMDFFRDGQAIGAFHAMKKG 240

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILT+ AAGNDGP+LSTVENVAPWIMTVAATAIDR FVT+F+LGNGNK TG SINTFSP+K
Sbjct: 241 ILTTAAAGNDGPQLSTVENVAPWIMTVAATAIDRGFVTSFELGNGNKLTGGSINTFSPQK 300

Query: 302 EMYSLTSGAK-ASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +MYSLTSGAK ASNN   +QGNASACDP AINQSKVKGKIVYCLKTYTDP IKSLGGTGV
Sbjct: 301 QMYSLTSGAKAASNNATPHQGNASACDPNAINQSKVKGKIVYCLKTYTDPSIKSLGGTGV 360

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQLT QQTDYSSILLLPGA+I S+SGKYIDLYINSTKNPKA+IYKSETVKIDAPFVASFS
Sbjct: 361 IQLTTQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 420

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTGD SDSRYS FTVMSGTSMACPHATA
Sbjct: 421 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACPHATA 480

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+KSKDA+LGSGAGQINPTKAVHPGLVYNISF
Sbjct: 481 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSKDAMLGSGAGQINPTKAVHPGLVYNISF 540

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           NSYISFLCKEGYNSTTIGLL G+KKYNC+KIKPAQGTDGLNYPTMHKQLS PSS I+AVF
Sbjct: 541 NSYISFLCKEGYNSTTIGLLGGSKKYNCTKIKPAQGTDGLNYPTMHKQLSGPSSTIEAVF 600

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVTHVGYGASLYRANISSP  +SVKVFPDTLNF KLHETKTFKVVVKGKPMP+GT+IL
Sbjct: 601 YRTVTHVGYGASLYRANISSPASLSVKVFPDTLNFVKLHETKTFKVVVKGKPMPKGTQIL 660

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIVRSNILIYR+LI
Sbjct: 661 SALLEWTDSKHIVRSNILIYRKLI 684

[Close]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match: A0A0A0KFB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=3 SV=1)

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 614/684 (89.77%), Postives = 647/684 (94.59%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           IG EKIARESKIYSYGKSFNGFAARLLP EATKLS+EE VVSVF+SRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLNH YSKRNP IESNLIVAV DT    DSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDT----DSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGANYFDL+ V+SYPE SVADT+GHGSH ASTVAGSAV GASLYGLAKGTARGGVPSA
Sbjct: 181 KVIGANYFDLDKVTSYPELSVADTDGHGSHIASTVAGSAVAGASLYGLAKGTARGGVPSA 240

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWS+FC+EMDVLA FDEAIADGVD ISVSIGSPPMDFFRDGQAIGAFHAMKKG
Sbjct: 241 RIAVYKVCWSVFCNEMDVLAAFDEAIADGVDLISVSIGSPPMDFFRDGQAIGAFHAMKKG 300

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILT+ AAGNDGPEL TVENVAPWIMTVAAT IDR FVTAF+LGNGNKFTG SINTFSP+K
Sbjct: 301 ILTTTAAGNDGPELFTVENVAPWIMTVAATGIDRGFVTAFELGNGNKFTGGSINTFSPQK 360

Query: 302 EMYSLTSGAKAS-NNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +M+SLTSGAKA+ NNG  +QGNASACDP A+NQSKVKGKIVYCLKTYTDP IKSLGGTGV
Sbjct: 361 QMHSLTSGAKAAFNNGTPHQGNASACDPNAMNQSKVKGKIVYCLKTYTDPSIKSLGGTGV 420

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQLTQQQTDYSSILLLPGA+I S+SGKYIDLYINSTKNPKA+IYKSETVKIDAPFVASFS
Sbjct: 421 IQLTQQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 480

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ ISSNILKPDLSAPGIDILAAYTKLA+LTGD SDSRYS FTVMSGTSMAC HATA
Sbjct: 481 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACSHATA 540

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+KS+D VLGSGAGQINPTKAVHPGLVYNISF
Sbjct: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDVVLGSGAGQINPTKAVHPGLVYNISF 600

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           +SYISFLCKEGYN+TTIGLL G+KKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAI+AVF
Sbjct: 601 DSYISFLCKEGYNNTTIGLLGGSKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIEAVF 660

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVTHVGYGASLYRANISSPD +SVKVFPDTLNF KLHET+TFKVVVKGKPMP+GT+IL
Sbjct: 661 YRTVTHVGYGASLYRANISSPDSLSVKVFPDTLNFVKLHETRTFKVVVKGKPMPKGTQIL 720

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIVRSNILIYR+ I
Sbjct: 721 SALLEWTDSKHIVRSNILIYREKI 740

[Close]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match: A0A6J1I5A0 (subtilisin-like protease SBT4.15 OS=Cucurbita maxima OX=3661 GN=LOC111471144 PE=3 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 576/682 (84.46%), Postives = 628/682 (92.08%), Query Frame = 0 

Query: 4   SEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGL 63
           SE  ARESKI+SYG+SFNGFAARLLPHEA KLSN EGVVSVF SRK RVVTTRSWDFLGL
Sbjct: 10  SENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWDFLGL 69

Query: 64  NHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKV 123
           NH  SKRNPKIESN+IVAVLDTGIWI+SPSFSDEGYGPPP KWKG+CVTGPNFTACNNKV
Sbjct: 70  NHR-SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKV 129

Query: 124 IGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARI 183
           IGANYFDL++VS + E+SVADTEGHGSHTASTVAGSAVEGASLYGL KGTARGGVPSARI
Sbjct: 130 IGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARI 189

Query: 184 AVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKGIL 243
           AVYKVCWSIFCS+MDVLAGFDEAIADGVDFISVSIGS  +DFFRD  AIGAFHAMKKGIL
Sbjct: 190 AVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGIL 249

Query: 244 TSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEM 303
           TSCAAGNDGPELSTV NVAPWIMTVAAT IDRRFVTAFKLGNG KFTGFSINTFSP+ +M
Sbjct: 250 TSCAAGNDGPELSTVVNVAPWIMTVAATGIDRRFVTAFKLGNGKKFTGFSINTFSPRNQM 309

Query: 304 YSLTSGAKASNNGGSYQ-GNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGVIQ 363
           YSLTSGAKASNNG + +  +ASACD +A++QSKVKG+IVYCL T+TD  I+SLGGTG+I+
Sbjct: 310 YSLTSGAKASNNGTTLRNASASACDADALSQSKVKGRIVYCLTTFTDYNIESLGGTGIIE 369

Query: 364 LTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFSSR 423
           L ++QTD S ILLLPGA I  +SGKYIDLYIN+TK+P+AIIYKS+TVKI APFVASFSSR
Sbjct: 370 LLEKQTDSSPILLLPGAVIPPVSGKYIDLYINATKDPRAIIYKSKTVKIAAPFVASFSSR 429

Query: 424 GPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATAAA 483
           GPQ IS NILKPDL+APGIDILAAYTKL+SLTG ++DSRYSLF+VM+GTSMACPHATAAA
Sbjct: 430 GPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAA 489

Query: 484 AYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISFNS 543
           AYVKSFHPDWSPAAVKSALMTTATPMK+KS+DA  GSGAGQ+NP KAVHPGLVY+IS +S
Sbjct: 490 AYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQVNPAKAVHPGLVYDISLDS 549

Query: 544 YISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVFYR 603
           YISFLCKEGYNSTTIGLL G+K+YNCSKIKPAQGTDGLNYPTMHKQLSDP SAI AVFYR
Sbjct: 550 YISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITAVFYR 609

Query: 604 TVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILSA 663
           TVTHV YGAS+YRANISSP G+SVKVFPD+L+FAK  E KTFKVVVKG+ M +GTRILSA
Sbjct: 610 TVTHVEYGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTRILSA 669

Query: 664 LLEWNDSKHIVRSNILIYRQLI 685
            LEW DSKH+VRSNILIYRQL+
Sbjct: 670 SLEWKDSKHVVRSNILIYRQLL 690

[Close]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match: A0A6J1HD77 (subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111463066 PE=3 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 577/683 (84.48%), Postives = 625/683 (91.51%), Query Frame = 0 

Query: 4   SEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGL 63
           SE  ARESKI+SYG+SFNGFAARLLPHEA KLSN EGVVSVF SRK RVVTTRSW+FLGL
Sbjct: 13  SENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWNFLGL 72

Query: 64  NHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKV 123
           N   SKRN KIESN+IVAVLDTGIWI+SPSFSDEGYGPPP KWKG+CVTGPNFTACNNKV
Sbjct: 73  NRR-SKRNLKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKV 132

Query: 124 IGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARI 183
           IGANYFDL++VS + E SVADTEGHGSHTASTVAGSAVEGASLYGL KGTARGGVPSARI
Sbjct: 133 IGANYFDLDNVSQFTEPSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARI 192

Query: 184 AVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKGIL 243
           AVYKVCWSIFCS+MDVLAGFDEAIADGVDFISVSIGS  +DFFRD  AIGAFHAMKKGIL
Sbjct: 193 AVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGIL 252

Query: 244 TSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEM 303
           TSCAAGNDGPELSTVENVAPWIMTVAAT IDRRFVTAFKLGN  KFTGFSINTFSP+ + 
Sbjct: 253 TSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFVTAFKLGNDKKFTGFSINTFSPRNQK 312

Query: 304 YSLTSGAKASNNGGSYQGN--ASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGVI 363
           YSLTSGAKASNN G+  GN  ASACD +A++QSKVKG+IVYCL T+TD  I+SLGGTG+I
Sbjct: 313 YSLTSGAKASNN-GTILGNASASACDVDALSQSKVKGRIVYCLTTFTDYNIESLGGTGII 372

Query: 364 QLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFSS 423
           +L ++QTD S ILLLPGA I  +SGKYIDLYINSTK+P+AIIYKS+TVKI APFVASFSS
Sbjct: 373 ELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFSS 432

Query: 424 RGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATAA 483
           RGPQ IS NILKPDL+APGIDILAAYTKL+SLTG ++DSRYSLF+VM+GTSMACPHATAA
Sbjct: 433 RGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAA 492

Query: 484 AAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISFN 543
           AAYVKSFHPDWSPAAVKSALMTTATPMK+KS+DA  GSGAGQINPTKAVHPGLVY+IS N
Sbjct: 493 AAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLN 552

Query: 544 SYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVFY 603
           SYISFLCKEGYNSTTIGLL G+K+YNCSKIKPAQGTDGLNYPTMHKQLSDP SAI AVFY
Sbjct: 553 SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITAVFY 612

Query: 604 RTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILS 663
           RTVTHV +GAS+YRANISSP G+SVKVFPD+L+FAK  E KTFKVVVKG+ M +GTRILS
Sbjct: 613 RTVTHVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTRILS 672

Query: 664 ALLEWNDSKHIVRSNILIYRQLI 685
           ALLEW  SKH+VRSNILIYRQL+
Sbjct: 673 ALLEWKGSKHVVRSNILIYRQLL 693

[Close]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match: A0A6J1CRC3 (subtilisin-like protease SBT4.15 OS=Momordica charantia OX=3673 GN=LOC111013614 PE=3 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 568/684 (83.04%), Postives = 619/684 (90.50%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           IG EK ARESKI+SYGKSFNGFAARLLPHEA K+S  E VVSVF+SRKQR+VTTRSWDFL
Sbjct: 60  IGDEKTARESKIHSYGKSFNGFAARLLPHEADKISKREDVVSVFRSRKQRIVTTRSWDFL 119

Query: 62  GLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 121
           GLN   SKRNP +E+NLIVAVLDTGIWI+SPSFSD+GYGPPP KWKGKCVTG NF+ACNN
Sbjct: 120 GLNKR-SKRNPSLEANLIVAVLDTGIWINSPSFSDKGYGPPPAKWKGKCVTGANFSACNN 179

Query: 122 KVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSA 181
           KVIGA YFDL+  +     SVADTEGHGSHTASTVAGSAVEGASLYGLA+GTARGGVPSA
Sbjct: 180 KVIGAAYFDLDDQNPAAARSVADTEGHGSHTASTVAGSAVEGASLYGLARGTARGGVPSA 239

Query: 182 RIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 241
           RIAVYKVCWSIFCSEMDVLAGFD+AIADGVD ISVSIGSPPMD FRD QAIGAFHAMKKG
Sbjct: 240 RIAVYKVCWSIFCSEMDVLAGFDQAIADGVDLISVSIGSPPMDLFRDSQAIGAFHAMKKG 299

Query: 242 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 301
           ILTS AAGN GP+LSTVENVAPWIMTVAATAIDRRF+T  +LGNG+KFTGFSINTFS  K
Sbjct: 300 ILTSSAAGNYGPQLSTVENVAPWIMTVAATAIDRRFITVLQLGNGSKFTGFSINTFSGGK 359

Query: 302 EMYSLTSGAKASN-NGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGV 361
           +M  LTSGAKA+N   GS +GNASACD EA++QSKVKG+IVYCL  Y DP IKSLGG GV
Sbjct: 360 QMQPLTSGAKAANATAGSNRGNASACDAEALSQSKVKGRIVYCLGNYADPNIKSLGGVGV 419

Query: 362 IQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASFS 421
           IQL Q QTDYSSIL+LPGA+I S+SGK +DLYINSTKNP+A+IYKS T+KI APFVASFS
Sbjct: 420 IQLLQPQTDYSSILVLPGAAIPSISGKSVDLYINSTKNPRAVIYKSRTIKITAPFVASFS 479

Query: 422 SRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATA 481
           SRGPQ I+ NILKPDL+APGIDILAAYT+LASLTGD SDSRYSLF VM+GTSMACPHATA
Sbjct: 480 SRGPQSITRNILKPDLAAPGIDILAAYTELASLTGDTSDSRYSLFKVMAGTSMACPHATA 539

Query: 482 AAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTKAVHPGLVYNISF 541
           AAAYVKSFHPDWSPAAVKSALMTTATPMK+K K+A LGSGAGQINPT+AVHPGLVY+IS 
Sbjct: 540 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKFKEAELGSGAGQINPTRAVHPGLVYDISL 599

Query: 542 NSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVF 601
           NSY+SFLCK+GYNST IGL+ G+KKYNCS +KPA+GTDGLNYPTMH+QLSDPSSAI AVF
Sbjct: 600 NSYVSFLCKQGYNSTAIGLIVGSKKYNCSMLKPARGTDGLNYPTMHQQLSDPSSAIDAVF 659

Query: 602 YRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRIL 661
           YRTVT+VGYGASLYRANI+SPDG+SVKVFPDTLNFAK HE KTFKVVVKG PMP+G +IL
Sbjct: 660 YRTVTNVGYGASLYRANITSPDGLSVKVFPDTLNFAKSHERKTFKVVVKGNPMPKGIQIL 719

Query: 662 SALLEWNDSKHIVRSNILIYRQLI 685
           SALLEW DSKHIV SNILI RQL+
Sbjct: 720 SALLEWTDSKHIVTSNILICRQLL 742

[Close]
BLAST of HG10016986 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 796.6 bits (2056), Expect = 1.6e-230
Identity = 405/701 (57.77%), Postives = 520/701 (74.18%), Query Frame = 0 

Query: 1   MIGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDF 60
           +IG E  ARE KIYSYGK+ NGF ARL PHEA KLS EEGVVSVF++ ++++ TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 61  LGLNHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LGL     KR+  IESN+IV VLDTGI ++SPSF+D+G GPPP KWKGKCVTG NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 121 NKVIGANYFDLESVSSYPE---SSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGG 180
           NKVIGA YF ++S    P+    + AD +GHG+HT+ST+AG +V  ASL+G+A GTARGG
Sbjct: 181 NKVIGAKYFHIQS-EGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGG 240

Query: 181 VPSARIAVYKVCWSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHA 240
           VPSARIA YKVCW   C++MD+LA FDEAI+DGVD IS+SIG   + FF D  AIGAFHA
Sbjct: 241 VPSARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHA 300

Query: 241 MKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTF 300
           MK+GILT+C+AGN+GP L TV N+APW+MTVAA ++DR+F T  KLGNG   +G S+N F
Sbjct: 301 MKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGF 360

Query: 301 SPKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLK---------TYT 360
           +P+K+MY LTSG+ ASN      G  S C+P  + + KV GK+VYC              
Sbjct: 361 NPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQ 420

Query: 361 DPYIKSLGGTGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSET 420
           D  ++SL G GVI    + TD ++  L+ G+ +    G  I  YINSTKNP+A+I+K++T
Sbjct: 421 DHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKT 480

Query: 421 VKIDAPFVASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVM 480
            K+ AP ++SFS+RGPQ IS NILKPD+SAPG++ILAAY+KLAS+TG   D+R +LF++M
Sbjct: 481 TKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIM 540

Query: 481 SGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKDAVLGSGAGQINPTK 540
           SGTSMACPHA AAAAYVKSFHPDWSPAA+KSALMTTATPM++K  +A L  G+GQINP +
Sbjct: 541 SGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRR 600

Query: 541 AVHPGLVYNISFNSYISFLCKEGYNSTTIGLLDGN-------KKYNCSKIKPAQGTDGLN 600
           A+HPGLVY+I+ ++Y+ FLCKEGYNST+IGLL G+       K+YNC  IK   G+DGLN
Sbjct: 601 AIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLN 660

Query: 601 YPTMHKQLSDPSSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHET 660
           YP++HKQ++   + +  VFYRTVT+VGYG S Y A + +P G+ V+V P  ++F +  E 
Sbjct: 661 YPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEK 720

Query: 661 KTFKVVVKGKPMPEGTRILSALLEWNDSK-HIVRSNILIYR 682
           + FKVV+ G        I+SA +EW+DS+ H+VRS IL++R
Sbjct: 721 RNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFR 760

[Close]
BLAST of HG10016986 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.5e-188
Identity = 350/682 (51.32%), Postives = 463/682 (67.89%), Query Frame = 0 

Query: 4   SEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGL 63
           S++ A+E K+YSY K+FN FAA+L PHEA K+   E VVSV +++ +++ TT+SWDF+GL
Sbjct: 66  SQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGL 125

Query: 64  NHHYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKV 123
               +KR+ K E ++I+ VLDTGI  DS SF D G GPPP KWKG C    NFT CNNK+
Sbjct: 126 -PLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKI 185

Query: 124 IGANYFDLE-SVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSAR 183
           IGA YF  + +V +    S  D +GHG+HT+STVAG  V  ASLYG+A GTARG VPSAR
Sbjct: 186 IGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSAR 245

Query: 184 IAVYKVCWS-IFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFFRDGQAIGAFHAMKKG 243
           +A+YKVCW+   C++MD+LAGF+ AI DGV+ IS+SIG P  D+  D  ++G+FHAM+KG
Sbjct: 246 LAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKG 305

Query: 244 ILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKK 303
           ILT  +AGNDGP   TV N  PWI+TVAA+ IDR F +   LGNG  F+G  I+ FSPK 
Sbjct: 306 ILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKA 365

Query: 304 EMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLK--TYTDPYIKSLGGTG 363
           + Y L SG  A+ N    +  A  C  +++++ KVKGK++ C       +  IKS GG G
Sbjct: 366 KSYPLVSGVDAAKNTDD-KYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAG 425

Query: 364 VIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETVKIDAPFVASF 423
            I ++ Q  D + I + P  S++S  G  I  YINST++  A+I K+  V I APFVASF
Sbjct: 426 AIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASF 485

Query: 424 SSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHAT 483
           SSRGP   S  +LKPD++APGIDILAA+T   SLTG   D+++S FT++SGTSMACPH  
Sbjct: 486 SSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVA 545

Query: 484 AAAAYVKSFHPDWSPAAVKSALMTTATPMKMK-SKDAVLGSGAGQINPTKAVHPGLVYNI 543
             AAYVKSFHPDW+PAA+KSA++T+A P+  + +KDA    G GQINP +A  PGLVY++
Sbjct: 546 GVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDM 605

Query: 544 SFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKA 603
              SY+ FLC EGYN+TT+  L G +  +CS I P  G D LNYPT+   L    ++  A
Sbjct: 606 DDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLA 665

Query: 604 VFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTR 663
           VF R VT+VG  +S+Y A + +P GV + V P +L+F+K  + ++FKVVVK K M  G +
Sbjct: 666 VFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPG-K 725

Query: 664 ILSALLEWNDSKHIVRSNILIY 681
           I+S LL W   +H VRS I+IY
Sbjct: 726 IVSGLLVWKSPRHSVRSPIVIY 744

[Close]
BLAST of HG10016986 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 555.8 bits (1431), Expect = 4.7e-158
Identity = 316/679 (46.54%), Postives = 433/679 (63.77%), Query Frame = 0 

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGLNHHYSKRNP 72
           + SY +SFNGFAA L   E+ KL N + VVSVF S+   + TTRSWDF+G      + + 
Sbjct: 33  VRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESV 92

Query: 73  KIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDLE 132
           K ES++IV V+D+GIW +S SF DEG+GPPP KWKG C  G  F ACNNK+IGA +++  
Sbjct: 93  K-ESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYNKF 152

Query: 133 SVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARIAVYKVCWSI 192
           +       S  D EGHG+HTAST AG+AV+ AS YGLA+GTARGGVPSARIA YKVC++ 
Sbjct: 153 A------DSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN- 212

Query: 193 FCSEMDVLAGFDEAIADGVDFISVSIGSPPM-DFFRDGQAIGAFHAMKKGILTSCAAGND 252
            C+++D+LA FD+AIADGVD IS+SI +  + +      AIG+FHAM +GI+T+ +AGN+
Sbjct: 213 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 272

Query: 253 GPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEMYSLTSGAK 312
           GP+  +V NV+PW++TVAA+  DR+F+    LGNG   TG S+NTF+     + +  G  
Sbjct: 273 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQN 332

Query: 313 ASNNGGSYQGNASACDPEAINQSKVKGKIVYC--LKTYTDPYIKSLGGTGVIQLTQQQTD 372
            S N    Q  A  C    ++   VKGKIV C     Y + Y+   G  GVI       D
Sbjct: 333 VSRN--CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLPD 392

Query: 373 YSSILLLPGASISSLSGKYIDLYINSTKNPKA-IIYKSETVKIDAPFVASFSSRGPQFIS 432
            + ++  P +S+     K I  YI S + P+A I+   E V  +AP+V SFSSRGP F+ 
Sbjct: 393 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 452

Query: 433 SNILKPDLSAPGIDILAAYTKLASLTGDMS--DSRYSLFTVMSGTSMACPHATAAAAYVK 492
            N+LKPD+SAPG++ILAA++ +AS +  ++  D R   ++VMSGTSMACPH    AAYVK
Sbjct: 453 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 512

Query: 493 SFHPDWSPAAVKSALMTTATPMKM-KSKDAVLGSGAGQINPTKAVHPGLVYNISFNSYIS 552
           SFHPDWSP+A+KSA+MTTATPM + K+ +     G+GQINPTKA  PGLVY +    Y+ 
Sbjct: 513 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 572

Query: 553 FLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLS--DPSSAIKAVFYRT 612
            LC EG++STT+    G +   CS+    +    LNYPTM   +S  DP       F RT
Sbjct: 573 MLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTMTTFVSSLDP---FNVTFKRT 632

Query: 613 VTHVGYGASLYRANI--SSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILS 672
           VT+VG+  S Y+A++    P+ + + + P+ L F  L E K+F V + GK + +G+ + S
Sbjct: 633 VTNVGFPNSTYKASVVPLQPE-LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSS 689

Query: 673 ALLEWNDSKHIVRSNILIY 681
           +++ W+D  H VRS I+ Y
Sbjct: 693 SVV-WSDGSHSVRSPIVAY 689

[Close]
BLAST of HG10016986 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 2.1e-150
Identity = 307/688 (44.62%), Postives = 424/688 (61.63%), Query Frame = 0 

Query: 2   IGSEKIARESKIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFL 61
           +  E   ++  + +Y +SFNGFAARL   E   L++ + VVSVF S+   + TT SW+F+
Sbjct: 60  VTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFM 119

Query: 62  GLNH-HYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 121
           GL     +KRNP IES+ I+ V+D+GI+ +S SFS +G+GPPP KWKG C  G NFT CN
Sbjct: 120 GLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CN 179

Query: 122 NKVIGANYFDLESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPS 181
           NK+IGA Y+    +  +PES+  D  GHGSHTAS  AG+AV+  S YGL  GT RGGVP+
Sbjct: 180 NKLIGARYY-TPKLEGFPESA-RDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPA 239

Query: 182 ARIAVYKVC--WSIFCSEMDVLAGFDEAIADGVDFISVSIGSPPMDFF-RDGQAIGAFHA 241
           ARIAVYKVC    I C+   +LA FD+AIAD VD I+VS+G+  +  F  D  AIGAFHA
Sbjct: 240 ARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHA 299

Query: 242 MKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTF 301
           M KGILT   AGN+GPE  T+ ++APW+ TVAA+ ++R F+T   LGNG    G S+N+F
Sbjct: 300 MAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSF 359

Query: 302 SPKKEMYSLTSGAKASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGG 361
               + Y L  G  AS+   +   +A  C P  ++  +VKGKIV C         +++G 
Sbjct: 360 DLNGKKYPLVYGKSASSRCDA--SSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGA 419

Query: 362 TGVIQLTQQQTDYSSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETV-KIDAPFV 421
              I +     D +S+   P + +S      +  Y+NSTKNPKA + KSET+    AP V
Sbjct: 420 VASI-VRNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVV 479

Query: 422 ASFSSRGPQFISSNILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACP 481
           AS+SSRGP  +  +ILKPD++APG +ILAAY+    +    SD+R+  +TV+SGTSM+CP
Sbjct: 480 ASYSSRGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCP 539

Query: 482 HATAAAAYVKSFHPDWSPAAVKSALMTTATPMKMKSKD----AVLGSGAGQINPTKAVHP 541
           H    AAY+K+FHP WSP+ ++SA+MTTA PM   +      A    GAG ++P  A+HP
Sbjct: 540 HVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHP 599

Query: 542 GLVYNISFNSYISFLCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDP 601
           GLVY  + + +I+FLC   Y    + L+ G+   +C+K +    T  LNYP+M  Q+S  
Sbjct: 600 GLVYEANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSG- 659

Query: 602 SSAIKAVFYRTVTHVGYGASLYRANISSPDGVSVKVFPDTLNFAKLHETKTFKVVVKGKP 661
           +   K  F RTVT+VG   + Y+A +     + VKV P  L+   L+E K+F V V G  
Sbjct: 660 TKPFKVTFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAG 719

Query: 662 MPEGTRILSALLEWNDSKHIVRSNILIY 681
            P+   ++SA L W+D  H VRS I++Y
Sbjct: 720 -PKAENLVSAQLIWSDGVHFVRSPIVVY 735

[Close]
BLAST of HG10016986 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 524.6 bits (1350), Expect = 1.2e-148
Identity = 303/680 (44.56%), Postives = 418/680 (61.47%), Query Frame = 0 

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFQSRKQRVVTTRSWDFLGLNH-HYSKRN 72
           + SY +SFNGFAARL   E T ++  EGVVSVF ++  ++ TT SWDF+G+     +KRN
Sbjct: 70  VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 129

Query: 73  PKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNNKVIGANYFDL 132
             IES+ I+ V+DTGIW +S SFSD+G+GPPP KWKG C  G NFT CNNK+IGA     
Sbjct: 130 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGAR---- 189

Query: 133 ESVSSYPESSVADTEGHGSHTASTVAGSAVEGASLYGLAKGTARGGVPSARIAVYKVCWS 192
                Y      DT GHG+HTAST AG+AV+  S +G+  GT RGGVP++RIA YKVC  
Sbjct: 190 ----DYTSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTD 249

Query: 193 IFCSEMDVLAGFDEAIADGVDFISVSIGSP-PMDFFRDGQAIGAFHAMKKGILTSCAAGN 252
             CS   +L+ FD+AIADGVD I++SIG   P  F  D  AIGAFHAM KGILT  +AGN
Sbjct: 250 SGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGN 309

Query: 253 DGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGNGNKFTGFSINTFSPKKEMYSLTSGA 312
            GP+ +TV +VAPWI TVAA+  +R F+T   LGNG    G S+N F  K + Y L  G 
Sbjct: 310 SGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGK 369

Query: 313 KASNNGGSYQGNASACDPEAINQSKVKGKIVYCLKTYTDPYIKSLGGTGVIQLTQQQTDY 372
            A+++    +  A+ C P  +N+S+VKGKI+ C         KS+G   +I     + D 
Sbjct: 370 SAASSACDAK-TAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIID-KSPRPDV 429

Query: 373 SSILLLPGASISSLSGKYIDLYINSTKNPKAIIYKSETV-KIDAPFVASFSSRGPQFISS 432
           +    LP + + +   K +  YI S  +P+A + K+ET+    +P +ASFSSRGP  I+ 
Sbjct: 430 AFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAV 489

Query: 433 NILKPDLSAPGIDILAAYTKLASLTGDMSDSRYSLFTVMSGTSMACPHATAAAAYVKSFH 492
           +ILKPD++APG++ILAA++     + D  D+R   ++V SGTSMACPH    AAYVK+F+
Sbjct: 490 DILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFY 549

Query: 493 PDWSPAAVKSALMTTATPMKMKSK---DAVLGSGAGQINPTKAVHPGLVYNISFNSYISF 552
           P WSP+ ++SA+MTTA P+K K +         GAG ++P  A++PGLVY +    +I+F
Sbjct: 550 PRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAF 609

Query: 553 LCKEGYNSTTIGLLDGNKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIKAVFYRTVTH 612
           LC   Y S T+ ++ G+    CSK K       LNYP+M  +LS   S     F RT+T+
Sbjct: 610 LCGMNYTSKTLKIISGD-TVKCSK-KNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTN 669

Query: 613 VGYGASLYRANISSPDG--VSVKVFPDTLNFAKLHETKTFKVVVKGKPMPEGTRILSALL 672
           VG   S Y++ + +  G  +S+KV P  L F  ++E ++F V V G  + +     SA L
Sbjct: 670 VGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDV-DSEVPSSANL 729

Query: 673 EWNDSKHIVRSNILIYRQLI 685
            W+D  H VRS I++Y  ++
Sbjct: 730 IWSDGTHNVRSPIVVYIMVV 733

The following BLAST results are available for this feature:
BLAST of HG10016986 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038882509.10.0e+0094.28subtilisin-like protease SBT4.15 [Benincasa hispida][more]
XP_016902874.10.0e+0091.37PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo][more]
XP_031743407.10.0e+0090.35subtilisin-like protease SBT4.15 isoform X1 [Cucumis sativus][more]
XP_031743408.10.0e+0089.18subtilisin-like protease SBT4.15 isoform X2 [Cucumis sativus][more]
XP_031743409.10.0e+0090.29subtilisin-like protease SBT4.15 isoform X3 [Cucumis sativus][more]
BLAST of HG10016986 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LZS62.2e-22957.77Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
Q9LLL82.1e-18751.32Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2... [more]
Q395471.8e-15745.61Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF86.7e-15746.54Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9STF73.0e-14944.62Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
BLAST of HG10016986 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S4E3S00.0e+0091.37subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=3 SV... [more]
A0A0A0KFB10.0e+0089.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=3 SV=1[more]
A0A6J1I5A00.0e+0084.46subtilisin-like protease SBT4.15 OS=Cucurbita maxima OX=3661 GN=LOC111471144 PE=... [more]
A0A6J1HD770.0e+0084.48subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111463066 P... [more]
A0A6J1CRC30.0e+0083.04subtilisin-like protease SBT4.15 OS=Momordica charantia OX=3673 GN=LOC111013614 ... [more]
BLAST of HG10016986 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G03620.11.6e-23057.77Subtilisin-like serine endopeptidase family protein [more]
AT4G00230.11.5e-18851.32xylem serine peptidase 1 [more]
AT5G59190.14.7e-15846.54subtilase family protein [more]
AT3G46850.12.1e-15044.62Subtilase family protein [more]
AT5G59090.11.2e-14844.56subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 144..157
score: 44.77
coord: 469..485
score: 49.84
coord: 75..94
score: 33.23
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 580..679
e-value: 1.9E-20
score: 72.9
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 1..52
e-value: 8.0E-12
score: 47.4
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 7..49
e-value: 5.4E-8
score: 33.4
NoneNo IPR availableGENE3D2.60.40.2310coord: 547..682
e-value: 3.7E-30
score: 106.3
NoneNo IPR availableGENE3D3.50.30.30coord: 274..409
e-value: 1.4E-167
score: 560.2
NoneNo IPR availablePANTHERPTHR10795:SF630CUCUMISIN PROTEINcoord: 2..681
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 57..530
score: 26.484814
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 281..403
e-value: 1.07877E-19
score: 83.6171
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 78..543
e-value: 1.4E-167
score: 560.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 68..530
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 76..517
e-value: 6.2E-51
score: 173.3
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 2..681
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 470..480
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 54..506
e-value: 5.06309E-120
score: 358.834

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016986.1HG10016986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity