HG10016937 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016937
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr03: 9493812 .. 9495203 (-)
RNA-Seq Expression	HG10016937
Synteny	HG10016937

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCTTCCCCATTCCCTTTCTCCTTTCCATCTTTCTCCTTCTTCCCCCTTCATCTTCTTCCCCTATCTCCACTATTACACTCCCCCTCACTGCCTTCCCTTCCAATTCACTTACAGATGATCCATGGAAAACCATCGATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAAGCCACAAACAAAATCAAACAGTTCCATCCAGAATGTCTCTCTGTTCCCTCGTAGCTATGGAGCTTATTCAATCTCACTTGCCTTCGGAACTCCACCGCAGAATTTATCGTTCATTTTCGATACTGGAAGTAGTGTCGTCTGGTTCCCCTGCACTGCTAGTTATCTTTGTTCTAATTGTTCGTTTCCTAATGTGGATGCTGCAACGATTCCGAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCGAAATGTGCTTGGATTTTTGGCCCTAATTTGAAATCTAGGTGTAGAAGTTGTAGCCCTAAATCTCGAAATTGTTTCGATTCTTGTCCTGGCTATGGAATTCAGTATGGCTCTGGTGCAACCGCTGGATTTCTCCTCTCTGAAACGCTTGATTTCCCGAAGAAACGAGTGCCGGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGGTCCTGAATCGTTGCCCTCGCAAATGCGGCTGAAACGATTCTCCTATTGCCTCGTTTCTCGTCGGTTCGACGACTCACCCGTGAGTAGTCCTCTAGTACTGAACTCCGGCTCGGAATCTAACGAATCGAAGAGTAAGAGTCTCATTTACGCACCGTTTCGAAAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCATCATCGGTGGAAAGCCGGTGAAGTTCCCGTACAAGTATCTTGTGCCGGATTCCGCCGGGAACGGCGGCGCGATCATTGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCATATCGGAAGAGTTGGAGAAGCAGCTGGTGAAATATCCTCGAGATAAGGGCGTTGAAGTGCAGTCCGGTTTAAGGCCGTGCTTCGATATTTCCAAGGAGAAATTGGCGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGCGAATTACTTGGCATTGGTGACGGATGACGGCGTGGTGTGCTTGACGATGATGACGGATGTAGCCGCCGTCGGCGGTGGCGGGGGGCCAGCGATTATATTCGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTATGATTTGGCGAGGGACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

mRNA sequence

ATGGAGTTCTTCCCCATTCCCTTTCTCCTTTCCATCTTTCTCCTTCTTCCCCCTTCATCTTCTTCCCCTATCTCCACTATTACACTCCCCCTCACTGCCTTCCCTTCCAATTCACTTACAGATGATCCATGGAAAACCATCGATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAAGCCACAAACAAAATCAAACAGTTCCATCCAGAATGTCTCTCTGTTCCCTCGTAGCTATGGAGCTTATTCAATCTCACTTGCCTTCGGAACTCCACCGCAGAATTTATCGTTCATTTTCGATACTGGAAGTAGTGTCGTCTGGTTCCCCTGCACTGCTAGTTATCTTTGTTCTAATTGTTCGTTTCCTAATGTGGATGCTGCAACGATTCCGAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCGAAATGTGCTTGGATTTTTGGCCCTAATTTGAAATCTAGGTGTAGAAGTTGTAGCCCTAAATCTCGAAATTGTTTCGATTCTTGTCCTGGCTATGGAATTCAGTATGGCTCTGGTGCAACCGCTGGATTTCTCCTCTCTGAAACGCTTGATTTCCCGAAGAAACGAGTGCCGGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGGTCCTGAATCGTTGCCCTCGCAAATGCGGCTGAAACGATTCTCCTATTGCCTCGTTTCTCGTCGGTTCGACGACTCACCCGTGAGTAGTCCTCTAGTACTGAACTCCGGCTCGGAATCTAACGAATCGAAGAGTAAGAGTCTCATTTACGCACCGTTTCGAAAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCATCATCGGTGGAAAGCCGGTGAAGTTCCCGTACAAGTATCTTGTGCCGGATTCCGCCGGGAACGGCGGCGCGATCATTGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCATATCGGAAGAGTTGGAGAAGCAGCTGGTGAAATATCCTCGAGATAAGGGCGTTGAAGTGCAGTCCGGTTTAAGGCCGTGCTTCGATATTTCCAAGGAGAAATTGGCGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGCGAATTACTTGGCATTGGTGACGGATGACGGCGTGGTGTGCTTGACGATGATGACGGATGTAGCCGCCGTCGGCGGTGGCGGGGGGCCAGCGATTATATTCGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTATGATTTGGCGAGGGACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

Coding sequence (CDS)

ATGGAGTTCTTCCCCATTCCCTTTCTCCTTTCCATCTTTCTCCTTCTTCCCCCTTCATCTTCTTCCCCTATCTCCACTATTACACTCCCCCTCACTGCCTTCCCTTCCAATTCACTTACAGATGATCCATGGAAAACCATCGATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAAGCCACAAACAAAATCAAACAGTTCCATCCAGAATGTCTCTCTGTTCCCTCGTAGCTATGGAGCTTATTCAATCTCACTTGCCTTCGGAACTCCACCGCAGAATTTATCGTTCATTTTCGATACTGGAAGTAGTGTCGTCTGGTTCCCCTGCACTGCTAGTTATCTTTGTTCTAATTGTTCGTTTCCTAATGTGGATGCTGCAACGATTCCGAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCGAAATGTGCTTGGATTTTTGGCCCTAATTTGAAATCTAGGTGTAGAAGTTGTAGCCCTAAATCTCGAAATTGTTTCGATTCTTGTCCTGGCTATGGAATTCAGTATGGCTCTGGTGCAACCGCTGGATTTCTCCTCTCTGAAACGCTTGATTTCCCGAAGAAACGAGTGCCGGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGGTCCTGAATCGTTGCCCTCGCAAATGCGGCTGAAACGATTCTCCTATTGCCTCGTTTCTCGTCGGTTCGACGACTCACCCGTGAGTAGTCCTCTAGTACTGAACTCCGGCTCGGAATCTAACGAATCGAAGAGTAAGAGTCTCATTTACGCACCGTTTCGAAAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCATCATCGGTGGAAAGCCGGTGAAGTTCCCGTACAAGTATCTTGTGCCGGATTCCGCCGGGAACGGCGGCGCGATCATTGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCATATCGGAAGAGTTGGAGAAGCAGCTGGTGAAATATCCTCGAGATAAGGGCGTTGAAGTGCAGTCCGGTTTAAGGCCGTGCTTCGATATTTCCAAGGAGAAATTGGCGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGCGAATTACTTGGCATTGGTGACGGATGACGGCGTGGTGTGCTTGACGATGATGACGGATGTAGCCGCCGTCGGCGGTGGCGGGGGGCCAGCGATTATATTCGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTATGATTTGGCGAGGGACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

Protein sequence

MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQHLKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT

Homology

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BLAST of HG10016937 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 820.8 bits (2119), Expect = 5.7e-234
Identity = 410/463 (88.55%), Postives = 427/463 (92.22%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFFPIPFL SIFLLLP SSSS ISTITLPLTAFPS  LTDDP K I+YLLSASLNRAQH
Sbjct: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK PQTK   SIQNVSLF RSYGAYSI+LAFGTPPQNLSF+FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNL SRCR+C+PKSRNC  SCP
Sbjct: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGI YGSGATAGFLLSETLDFPKK VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFSYCLVSR FDDSPVSSPLVL+SGSES++SK++S IYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           SLRRI+IGGKPVK PYKYL+PDSAG GGAIIDSGSTFTFLDKPIFEA++ ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R K VEVQSGLRPCFDISKE   EFPELVLKFKGGAKLSLPP NYLALVTD GVVCLTMM
Sbjct: 361 RTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMM 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  VGGG GPAIIFGAFQQQNVLVEYDLAR+RIGFRKQRCT
Sbjct: 421 TDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 460

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BLAST of HG10016937 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.5 bits (2061), Expect = 3.0e-227
Identity = 392/463 (84.67%), Postives = 426/463 (92.01%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFFPIPFLLSI LLL  SSSS  +T+TLPLT FPS   T  PWK I +L+SASL RAQH
Sbjct: 1   MEFFPIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFT-HPWKNIKHLVSASLTRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+ KSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSAKIIGCRN KC+WIFGPNLKS CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S SES ESK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPPANYLALVTD GVVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA+DRIGFRKQRCT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKDRIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 793.9 bits (2049), Expect = 7.5e-226
Identity = 391/463 (84.45%), Postives = 426/463 (92.01%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFFPI FLLSI LLL  SSSS   T+TLPLTAFPS  LT  PWK I +L+SASL RAQH
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLT-HPWKNIKHLVSASLARAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+TKSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSA+IIGCRN KC+WIFGPNLKS CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S  ES +SK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPPANYLALVTD GVVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA++RIGFRKQRCT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 786.9 bits (2031), Expect = 9.2e-224
Identity = 386/463 (83.37%), Postives = 423/463 (91.36%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFF IPFLLSI LLL  SSSS  +T+TLPLT FPS      PWK I +L+SASL RAQH
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFA-HPWKNIKHLVSASLTRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+TKSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSAKIIGCRN KC+WIFGPNLK+ CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S SES ESK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPP+NYLALV D  VVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA++RIGFRKQRCT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. NCBI nr
Match: KAG7034471.1 (Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 386/463 (83.37%), Postives = 423/463 (91.36%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFF IPFLLSI LLL  SSSS  +T+TLPLT FPS      PWK I +L+SASL RAQH
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFA-HPWKNIKHLVSASLTRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+TKSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKIPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSAKIIGCRN KC+WIFGPNLK+ CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S SES ESK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPP+NYLALV D  VVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA++RIGFRKQRCT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 146/485 (30.10%), Postives = 225/485 (46.39%), Query Frame = 0


Query: 24  ISTITLPLTAFPSNSLTDDPWKT--IDYLLSASLNRAQHLKKPQTKSNSSIQNVSLFPRS 83
           +S+++ PL    S+SL+     +  +  L S+S   +   ++   K     Q +SL   S
Sbjct: 22  VSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQ--QQLSLPISS 81

Query: 84  YGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVP-KLS 143
              Y ISL+ G+    +S   DTGS +VWFPC   + C  C     ++  +P   P  LS
Sbjct: 82  GSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILC-----ESKPLPPSPPSSLS 141

Query: 144 SSAKIIGCRNPKCAWIFG--PNLK----SRCRSCSPKSRNCFDS---CPGYGIQYGSGAT 203
           SSA  + C +P C+      P+      S C     ++ +C  S   CP +   YG G+ 
Sbjct: 142 SSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL 201

Query: 204 AGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMRL------KRFS 263
              L S++L  P   V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS
Sbjct: 202 VAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFS 261

Query: 264 YCLVSRRFDDSPV--SSPLVL----------------NSGSESNESKSKSLIYAPFRKNP 323
           YCLVS  FD   V   SPL+L                +   +  + K    ++    +NP
Sbjct: 262 YCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENP 321

Query: 324 SGSNAAFREYYYLSLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEA 383
                    +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + +
Sbjct: 322 K-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 381

Query: 384 ISEELEKQLVK-YPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGG-AKLSLPPANY 443
           + EE + ++ + + R   VE  SG+ PC+ ++  +  + P LVL F G  + ++LP  NY
Sbjct: 382 VVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPRRNY 441

Query: 444 LALVTDDG--------VVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGF 463
                D G        + CL +M         GG   I G +QQQ   V YDL   R+GF
Sbjct: 442 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 491

[Close]

BLAST of HG10016937 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.7e-35
Identity = 146/478 (30.54%), Postives = 196/478 (41.00%), Query Frame = 0


Query: 19  SSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNR-------------------AQ 78
           S S   S+ITL L    + S      KT D L S+ L R                     
Sbjct: 64  SDSESSSSITLNLDHIDALSSN----KTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVT 123

Query: 79  HLKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLC 138
           H  +P   S+S +  +S   +  G Y   L  GTP + +  + DTGS +VW  C     C
Sbjct: 124 HAPRPGGFSSSVVSGLS---QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC 183

Query: 139 SNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSC 198
            + S P  D        P+ S +   I C +P C  +      +R ++C           
Sbjct: 184 YSQSDPIFD--------PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCL---------- 243

Query: 199 PGYGIQYGSGA-TAGFLLSETLDFPKKRVPDFLVGCSVLSVHQ-------PAGIAGFGRG 258
             Y + YG G+ T G   +ETL F + RV    +GC     H         AG+ G G+G
Sbjct: 244 --YQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCG----HDNEGLFVGAAGLLGLGKG 303

Query: 259 PESLPSQMRLK---RFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPS 318
             S P Q   +   +FSYCLV R     P S           N + S+   + P   NP 
Sbjct: 304 KLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVF-------GNAAVSRIARFTPLLSNPK 363

Query: 319 GSNAAFREYYYLSLRRIIIGGKPVKFPYKYLVP-DSAGNGGAIIDSGSTFTFLDKPIFEA 378
                   +YY+ L  I +GG  V      L   D  GNGG IIDSG++ T L +P + A
Sbjct: 364 -----LDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIA 423

Query: 379 ISEELE---KQLVKYPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPAN 438
           + +      K L + P        S    CFD+S     + P +VL F+ GA +SLP  N
Sbjct: 424 MRDAFRVGAKTLKRAPD------FSLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATN 483

Query: 439 YLALVTDDGVVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRC 463
           YL  V  +G  C       A  G  GG +II G  QQQ   V YDLA  R+GF    C
Sbjct: 484 YLIPVDTNGKFCF------AFAGTMGGLSII-GNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of HG10016937 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.7e-35
Identity = 121/387 (31.27%), Postives = 171/387 (44.19%), Query Frame = 0


Query: 83  GAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSS 142
           G Y +++A GTP  + S I DTGS ++W  C     C+ C      +   P F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCF-----SQPTPIFNPQDSSS 153

Query: 143 AKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGSGATA-GFLLSETLD 202
              + C +  C  +               S  C ++   Y   YG G+T  G++ +ET  
Sbjct: 154 FSTLPCESQYCQDL--------------PSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 203 FPKKRVPDFLVGCSV----LSVHQPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 262
           F    VP+   GC            AG+ G G GP SLPSQ+ + +FSYC+ S     S 
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSY---GSS 273

Query: 263 VSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIIIGGKPVKFPYK 322
             S L L S +      S S        NP+        YYY++L+ I +GG  +  P  
Sbjct: 274 SPSTLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSS 333

Query: 323 YLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEVQSGLRPCF-D 382
                  G GG IIDSG+T T+L +  + A+++    Q+     D   E  SGL  CF  
Sbjct: 334 TFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVD---ESSSGLSTCFQQ 393

Query: 383 ISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMMTDVAAVGGGGGPAI-I 442
            S     + PE+ ++F GG  L+L   N L +   +GV+CL       A+G      I I
Sbjct: 394 PSDGSTVQVPEISMQFDGGV-LNLGEQNIL-ISPAEGVICL-------AMGSSSQLGISI 435

Query: 443 FGAFQQQNVLVEYDLARDRIGFRKQRC 463
           FG  QQQ   V YDL    + F   +C
Sbjct: 454 FGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of HG10016937 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.7e-34
Identity = 117/387 (30.23%), Postives = 171/387 (44.19%), Query Frame = 0


Query: 83  GAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSS 142
           G Y ++L+ GTP Q  S I DTGS ++W  C     C N S         P F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQS--------TPIFNPQGSSS 152

Query: 143 AKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGSGA-TAGFLLSETLD 202
              + C +  C  +  P               C ++   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 203 FPKKRVPDFLVGCSV----LSVHQPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 262
           F    +P+   GC            AG+ G GRGP SLPSQ+ + +FSYC+       S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP---IGSS 272

Query: 263 VSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIIIGGKPVKF-PY 322
             S L+L S + S  + S +       + P+        +YY++L  + +G   +   P 
Sbjct: 273 TPSNLLLGSLANSVTAGSPNTTLIQSSQIPT--------FYYITLNGLSVGSTRLPIDPS 332

Query: 323 KYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEVQSGLRPCFD 382
            + +  + G GG IIDSG+T T+     ++++ +E   Q +  P   G    SG   CF 
Sbjct: 333 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLCFQ 392

Query: 383 I-SKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMMTDVAAVGGGGGPAII 442
             S     + P  V+ F GG  L LP  NY  +   +G++CL       A+G       I
Sbjct: 393 TPSDPSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICL-------AMGSSSQGMSI 434

Query: 443 FGAFQQQNVLVEYDLARDRIGFRKQRC 463
           FG  QQQN+LV YD     + F   +C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of HG10016937 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.8e-28
Identity = 111/424 (26.18%), Postives = 166/424 (39.15%), Query Frame = 0


Query: 51  LSASLNRAQHLKKPQTKSNSSIQN-----VSLFPRSYGAYSISLAFGTPPQNLSFIFDTG 110
           +SA L R      P + S   + +     VS   +  G Y + +  G+PP++   + D+G
Sbjct: 92  VSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSG 151

Query: 111 SSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRC 170
           S +VW  C    LC   S P  D        P  S S   + C +  C  I         
Sbjct: 152 SDMVWVQCQPCKLCYKQSDPVFD--------PAKSGSYTGVSCGSSVCDRI--------- 211

Query: 171 RSCSPKSRNCFDSCPGYGIQYGSGA-TAGFLLSETLDFPKKRVPDFLVGCSVLSVHQ--- 230
                ++  C      Y + YG G+ T G L  ETL F K  V +  +GC   +      
Sbjct: 212 -----ENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIG 271

Query: 231 PAGIAGFGRGPESLPSQMRLK---RFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSL 290
            AG+ G G G  S   Q+  +    F YCLVSR  D    +  LV        E+     
Sbjct: 272 AAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS---TGSLVF-----GREALPVGA 331

Query: 291 IYAPFRKNPSGSNAAFREYYYLSLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFT 350
            + P  +NP   +     +YY+ L+ + +GG  +  P         G+GG ++D+G+  T
Sbjct: 332 SWVPLVRNPRAPS-----FYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVT 391

Query: 351 FLDKPIFEAISEELEKQLVKYPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKL 410
            L    + A  +  + Q    PR  GV +      C+D+S       P +   F  G  L
Sbjct: 392 RLPTAAYVAFRDGFKSQTANLPRASGVSI---FDTCYDLSGFVSVRVPTVSFYFTEGPVL 451

Query: 411 SLPPANYLALVTDDGVVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFR 463
           +LP  N+L  V D G  C         +        I G  QQ+ + V +D A   +GF 
Sbjct: 452 TLPARNFLMPVDDSGTYCFAFAASPTGLS-------IIGNIQQEGIQVSFDGANGFVGFG 470

[Close]

BLAST of HG10016937 vs. ExPASy TrEMBL
Match: A0A6J1IMR7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 391/463 (84.45%), Postives = 426/463 (92.01%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFFPI FLLSI LLL  SSSS   T+TLPLTAFPS  LT  PWK I +L+SASL RAQH
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLT-HPWKNIKHLVSASLARAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+TKSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSA+IIGCRN KC+WIFGPNLKS CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S  ES +SK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPPANYLALVTD GVVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA++RIGFRKQRCT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. ExPASy TrEMBL
Match: A0A6J1EDJ0 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111433208 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.4e-224
Identity = 386/463 (83.37%), Postives = 423/463 (91.36%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEFF IPFLLSI LLL  SSSS  +T+TLPLT FPS      PWK I +L+SASL RAQH
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFA-HPWKNIKHLVSASLTRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK P+TKSN+SIQNV+LFPRSYGAYSISLAFGTPPQ+LS +FDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           NCSFPNVDAATIPKF+PKLSSSAKIIGCRN KC+WIFGPNLK+ CRSCSP+SR C D+CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAGFLLSETLDFP+KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLV R+FDDSPVSSPLVL+S SES ESK+ SLIYAPFR+NPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           +LRRI+IG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEA++EELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMM 420
           R KGVE +SGLRPCFDISKE+  EFPEL+LKFKGGA L+LPP+NYLALV D  VVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMI 420

Query: 421 TDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           TDV  +GGGGGPAIIFGAFQQQNVLV+YDLA++RIGFRKQRCT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of HG10016937 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.2e-222
Identity = 391/464 (84.27%), Postives = 419/464 (90.30%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEF PIPFL SIFLLLP SSSS  ST  LPLT FPS S T DP+KTI+ LLSASLNRAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSS--STTVLPLTTFPSVSFT-DPFKTINLLLSASLNRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK PQ+KSN+SIQNVSLFPRSYGAYS+SLAFGTPPQNLSFIFDTGSS+VWFPCTA Y CS
Sbjct: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
            CSFP VD ATI KFVPKLSSS K++GCRNPKCAWIFGPNLKSRCR+C+ KSR C DSCP
Sbjct: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQMR
Sbjct: 181 GYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CLVSR FDDSPVSSPLVL+SGSES+ESK+KS IYAPFR+NPS SNAAFREYYYL
Sbjct: 241 LKRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           SLRRI+IGGKPVKFPYKYLVPDS GNGGAIIDSGSTFTFLDKPIFEAI++ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISK-EKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTM 420
           R K VE QSGLRPCF+I K E+ AEFP++VLKFKGG KLSL   NYLA+VTD+GVVCLTM
Sbjct: 361 RAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTM 420

Query: 421 MTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           MTD A VGGGGGPAII GAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of HG10016937 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.9e-216
Identity = 377/464 (81.25%), Postives = 413/464 (89.01%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEF PIPFL SIFLLLP SSS   S+ITLPL  FPS   T DP KTI++LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSS---SSITLPLATFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK PQ+KSN+S +NVSLFPRSYGAY++SLAFGTPPQNLSFIFDTGSS+VWFPCTA Y C+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNLKSRCR+C+PKSR C DSCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CL+ R FDDSPVSSPLVL+SG ES+ESK+KS IYAPF++NPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           SLRRI+IGGKPVKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEAI+ ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISK-EKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTM 420
           R K +E ++GLRPCF+ISK E+ AEFPE+ LKFKGG KLSLPP NYL +VTD  VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           MT+   VG GGGPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of HG10016937 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.9e-216
Identity = 377/464 (81.25%), Postives = 413/464 (89.01%), Query Frame = 0


Query: 1   MEFFPIPFLLSIFLLLPPSSSSPISTITLPLTAFPSNSLTDDPWKTIDYLLSASLNRAQH 60
           MEF PIPFL SIFLLLP SSS   S+ITLPL  FPS   T DP KTI++LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSS---SSITLPLATFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCS 120
           LK PQ+KSN+S +NVSLFPRSYGAY++SLAFGTPPQNLSFIFDTGSS+VWFPCTA Y C+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNLKSRCR+C+PKSR C DSCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMR 240
           GYGIQYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYL 300
           LKRFS+CL+ R FDDSPVSSPLVL+SG ES+ESK+KS IYAPF++NPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYP 360
           SLRRI+IGGKPVKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEAI+ ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RDKGVEVQSGLRPCFDISK-EKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTM 420
           R K +E ++GLRPCF+ISK E+ AEFPE+ LKFKGG KLSLPP NYL +VTD  VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
           MT+   VG GGGPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of HG10016937 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 495.7 bits (1275), Expect = 3.9e-140
Identity = 246/457 (53.83%), Postives = 324/457 (70.90%), Query Frame = 0


Query: 22  SPISTITLPLTAFP-SNSLTDDPWKTIDYLLSASLNRAQHLK-----KPQ-------TKS 81
           S +S + LPL+ F  S+    DP+ ++  L  +S+ RA  LK     KP        T +
Sbjct: 14  SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSIKPDEDALSSTTTA 73

Query: 82  NSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVD 141
           ++++    L  +SYG YS+SL+FGTP Q + F+FDTGSS+VW PCT+ YLCS C F  +D
Sbjct: 74  SATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTSRYLCSGCDFSGLD 133

Query: 142 AATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGS 201
              IP+F+PK SSS+KIIGC++PKC +++GPN+  +CR C P +RNC   CP Y +QYG 
Sbjct: 134 PTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNCTVGCPPYILQYGL 193

Query: 202 GATAGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMRLKRFSYCL 261
           G+TAG L++E LDFP   VPDF+VGCS++S  QPAGIAGFGRGP SLPSQM LKRFS+CL
Sbjct: 194 GSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNLKRFSHCL 253

Query: 262 VSRRFDDSPVSSPLVLNSGSESNE-SKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIII 321
           VSRRFDD+ V++ L L++GS  N  SK+  L Y PFRKNP+ SN AF EYYYL+LRRI +
Sbjct: 254 VSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYV 313

Query: 322 GGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEV 381
           G K VK PYKYL P + G+GG+I+DSGSTFTF+++P+FE ++EE   Q+  Y R+K +E 
Sbjct: 314 GRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQMSNYTREKDLEK 373

Query: 382 QSGLRPCFDISKEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMMTD-VAAV 441
           ++GL PCF+IS +     PEL+ +FKGGAKL LP +NY   V +   VCLT+++D     
Sbjct: 374 ETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTDTVCLTVVSDKTVNP 433

Query: 442 GGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRCT 464
            GG GPAII G+FQQQN LVEYDL  DR GF K++C+
Sbjct: 434 SGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of HG10016937 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-49
Identity = 138/406 (33.99%), Postives = 190/406 (46.80%), Query Frame = 0


Query: 85  YSISLAFGTPPQNLSFIFDTGSSVVWFPC-TASYLCSNC-SFPNVDAATIPKFVPKLSSS 144
           Y I+L  GTPPQ +    DTGS + W PC   S+ C  C    N D  +   F P  SS+
Sbjct: 83  YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSPLHSST 142

Query: 145 AKIIGCRNPKCAWI------FGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGSGA-TAGFL 204
           +    C +  C  I      F P   + C         C   CP +   YG G   +G L
Sbjct: 143 SFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCPSFAYTYGEGGLISGIL 202

Query: 205 LSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMRL--KRFSYCLVSRRF 264
             + L    + VP F  GC   +  +P GIAGFGRG  SLPSQ+    K FS+C +  +F
Sbjct: 203 TRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKF 262

Query: 265 DDSP-VSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIIIGGK-- 324
            ++P +SSPL+L + S  + + + SL + P    P   N+     YY+ L  I IG    
Sbjct: 263 VNNPNISSPLILGA-SALSINLTDSLQFTPMLNTPMYPNS-----YYIGLESITIGTNIT 322

Query: 325 PVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEVQSG 384
           P + P      DS GNGG ++DSG+T+T L +P +  +   L+   + YPR    E ++G
Sbjct: 323 PTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ-STITYPRATETESRTG 382

Query: 385 LRPCF----------DISKEKLAEFPELVLKFKGGAKLSLPPAN--YLALVTDDG--VVC 444
              C+           +  + +  FP +   F   A L LP  N  Y      DG  V C
Sbjct: 383 FDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQGNSFYAMSAPSDGSVVQC 442

Query: 445 LTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRC 463
           L          G  GPA +FG+FQQQNV V YDL ++RIGF+   C
Sbjct: 443 LLFQN---MEDGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

[Close]

BLAST of HG10016937 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-47
Identity = 146/485 (30.10%), Postives = 225/485 (46.39%), Query Frame = 0


Query: 24  ISTITLPLTAFPSNSLTDDPWKT--IDYLLSASLNRAQHLKKPQTKSNSSIQNVSLFPRS 83
           +S+++ PL    S+SL+     +  +  L S+S   +   ++   K     Q +SL   S
Sbjct: 22  VSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQ--QQLSLPISS 81

Query: 84  YGAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVP-KLS 143
              Y ISL+ G+    +S   DTGS +VWFPC   + C  C     ++  +P   P  LS
Sbjct: 82  GSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILC-----ESKPLPPSPPSSLS 141

Query: 144 SSAKIIGCRNPKCAWIFG--PNLK----SRCRSCSPKSRNCFDS---CPGYGIQYGSGAT 203
           SSA  + C +P C+      P+      S C     ++ +C  S   CP +   YG G+ 
Sbjct: 142 SSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL 201

Query: 204 AGFLLSETLDFPKKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMRL------KRFS 263
              L S++L  P   V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS
Sbjct: 202 VAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFS 261

Query: 264 YCLVSRRFDDSPV--SSPLVL----------------NSGSESNESKSKSLIYAPFRKNP 323
           YCLVS  FD   V   SPL+L                +   +  + K    ++    +NP
Sbjct: 262 YCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENP 321

Query: 324 SGSNAAFREYYYLSLRRIIIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEA 383
                    +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + +
Sbjct: 322 K-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 381

Query: 384 ISEELEKQLVK-YPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKFKGG-AKLSLPPANY 443
           + EE + ++ + + R   VE  SG+ PC+ ++  +  + P LVL F G  + ++LP  NY
Sbjct: 382 VVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPRRNY 441

Query: 444 LALVTDDG--------VVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGF 463
                D G        + CL +M         GG   I G +QQQ   V YDL   R+GF
Sbjct: 442 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 491

[Close]

BLAST of HG10016937 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 166.8 bits (421), Expect = 4.2e-41
Identity = 135/430 (31.40%), Postives = 183/430 (42.56%), Query Frame = 0


Query: 45  KTIDYLLSASLNRAQHLKKPQTKSNSSIQNVSLFPRSYGAYSISLAFGTPPQNLSFIFDT 104
           K+I  L + S  R    + P+T    S   +S   +  G Y + L  GTP  N+  + DT
Sbjct: 95  KSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDT 154

Query: 105 GSSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLKSR 164
           GS VVW  C+    C N      DA     F PK S +   + C +  C       L   
Sbjct: 155 GSDVVWLQCSPCKACYN----QTDAI----FDPKKSKTFATVPCGSRLCR-----RLDDS 214

Query: 165 CRSCSPKSRNCFDSCPGYGIQYGSGA-TAGFLLSETLDFPKKRVPDFLVGCSVLSVHQ-- 224
               + +S+ C      Y + YG G+ T G   +ETL F   RV    +GC     H   
Sbjct: 215 SECVTRRSKTCL-----YQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCG----HDNE 274

Query: 225 -----PAGIAGFGRGPESLPSQMRLK---RFSYCLVSRRFDDSPVSSPLVLNSGSESNES 284
                 AG+ G GRG  S PSQ + +   +FSYCLV R    S    P  +  G   N +
Sbjct: 275 GLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFG---NAA 334

Query: 285 KSKSLIYAPFRKNPSGSNAAFREYYYLSLRRIIIGGKPVK-FPYKYLVPDSAGNGGAIID 344
             K+ ++ P   NP         +YYL L  I +GG  V          D+ GNGG IID
Sbjct: 335 VPKTSVFTPLLTNPK-----LDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIID 394

Query: 345 SGSTFTFLDKPIFEAISEELEKQLVKYPRDKGVEVQSGLRPCFDISKEKLAEFPELVLKF 404
           SG++ T L +P + A+ +       K  R     +      CFD+S     + P +V  F
Sbjct: 395 SGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL---FDTCFDLSGMTTVKVPTVVFHF 454

Query: 405 KGGAKLSLPPANYLALVTDDGVVCLTMMTDVAAVGGGGGPAIIFGAFQQQNVLVEYDLAR 463
            GG ++SLP +NYL  V  +G  C        A  G  G   I G  QQQ   V YDL  
Sbjct: 455 -GGGEVSLPASNYLIPVNTEGRFCF-------AFAGTMGSLSIIGNIQQQGFRVAYDLVG 483

[Close]

BLAST of HG10016937 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.1e-39
Identity = 124/399 (31.08%), Postives = 187/399 (46.87%), Query Frame = 0


Query: 83  GAYSISLAFGTPPQNLSFIFDTGSSVVWFPCTASYLCSNCSFPNVDAATIPKFVPKLSSS 142
           G Y + +  GTPP++ S I DTGS + W  C   Y C + +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 143 AKIIGCRNPKCAWIFGPNLKSRCRSCSPKSRNCFDSCPGYGIQYGSGA-TAGFLLSETLD 202
            K I C +P+C+ I  P+   +C S +        SCP Y   YG  + T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 203 F---------PKKRVPDFLVGCSVLS---VHQPAGIAGFGRGPESLPSQMRL---KRFSY 262
                      + +V + + GC   +       +G+ G GRGP S  SQ++      FSY
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 263 CLVSRRFDDSPVSSPLVLNSGSESNESKSKSLIYAPFRKNPSGSNAAFREYYYLSLRRII 322
           CLV R   ++ VSS L+   G + +     +L +  F    +G   +   +YY+ ++ I+
Sbjct: 338 CLVDRN-SNTNVSSKLIF--GEDKDLLNHTNLNFTSF---VNGKENSVETFYYIQIKSIL 397

Query: 323 IGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAISEEL-EKQLVKYPRDKGV 382
           +GGK +  P +     S G+GG IIDSG+T ++  +P +E I  +  EK    YP  +  
Sbjct: 398 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF 457

Query: 383 EVQSGLRPCFDIS--KEKLAEFPELVLKFKGGAKLSLPPANYLALVTDDGVVCLTMMTDV 442
            V   L PCF++S  +E     PEL + F  G   + P  N    +++D +VCL      
Sbjct: 458 PV---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCL------ 517

Query: 443 AAVGGGGGPAIIFGAFQQQNVLVEYDLARDRIGFRKQRC 463
           A +G       I G +QQQN  + YD  R R+GF   +C
Sbjct: 518 AILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

The following BLAST results are available for this feature:

BLAST of HG10016937 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881211.1	5.7e-234	88.55	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_023543736.1	3.0e-227	84.67	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022979057.1	7.5e-226	84.45	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
XP_022925946.1	9.2e-224	83.37	probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic...	[more]
KAG7034471.1	1.6e-223	83.37	Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of HG10016937 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	1.9e-46	30.10	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	5.7e-35	30.54	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	9.7e-35	31.27	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	1.7e-34	30.23	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LHE3	1.8e-28	26.18	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of HG10016937 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IMR7	3.6e-226	84.45	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813...	[more]
A0A6J1EDJ0	4.4e-224	83.37	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A0A0KHK2	4.2e-222	84.27	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G45447...	[more]
A0A5A7SGF9	9.9e-216	81.25	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CHV2	9.9e-216	81.25	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]

BLAST of HG10016937 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	3.9e-140	53.83	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	4.2e-49	33.99	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	1.3e-47	30.10	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	4.2e-41	31.40	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	5.1e-39	31.08	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 329..340 score: 36.31 coord: 91..111 score: 51.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 266..463 e-value: 8.8E-49 score: 167.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 66..259 e-value: 1.2E-31 score: 112.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 80..462
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 85..255 e-value: 3.5E-28 score: 99.0
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 298..458 e-value: 1.6E-33 score: 115.8
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 8..462
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 8..462
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 100..111
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 85..458 score: 34.975674
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 84..462 e-value: 2.27813E-79 score: 245.635

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016937.1	HG10016937.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity