HG10016489 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016489
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytochrome P450 family protein
Location	Chr03: 5434282 .. 5435620 (+)
RNA-Seq Expression	HG10016489
Synteny	HG10016489

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGATTTGGATCTTTCTTATCATCATTCATCTGCTTGTTCTTCCATTTGTCCTTTGGCCATGGCTACTGAGGAGTAACAAAACCAAGAAATTACTGCCTCCTGGTCCAAAAGGGTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGGAAGCTTCCTCACAGAGATTTTCACCGCTTGTCACAAAGGTATGGAGGCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCTTCCCCTGAAGTCGCAGAGCTGTTTCTCAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGACGTTTTCAAGCACATGTATTTTGGCCAAAACAGCATTGCGGTTGAAAAATATGGGCCTTATTTACGCAATGTGCGCAAAATATGCACTCTGGAGTTGCTTAGCAGCAAGAAACTCAATTCTTTCAAAGCAATGAGAAGGGAAGAAGTTGGGTTGTTGATTGAGGAGCTTCGTGAGGCTGCCAACAGTGGCGTGGTTGTAAATCTTACTTCTAAAGTCTCTTCTCTCAGTGCAAAGATGATATGTCTAATGATCTTTGGTAGGAAATTTGAAGACGAAGAAATTGATGAAAGGGGTTTTAAGTGGATGGTACAAGAAGCCTTCAAAGTAGTTGGAGCTTTTAACATGGGGGACTTCATTCCTTTTATTGCACCTCTTGATCTTCAAGGATTGATCCGTCGTGCAAAATCTGTTCATAAAGTATTTGATAAGTTTCTTGAAAGGATCATAGATGAACATCTTCAATCCAAGAATAATGATACCAAAGATTTCGTAGAAGTCATGTTGGACATTATGGGATCACAAGAAACCGACTACCAAATTGATCGATCCACCATCAAAGCCATCATGCTGGTAAGTAAACATTTAGTAAGATGGTGTCTCTTTAGGCTATAATACCATAGTAAATAATATGTTTTCGCAAATTTATTTTTAATTTGATATTATATTAATTAATATGGAGCTTCATCTCAAAATTGATTGACAATAAGTAGAGTAGTTGATTATCTTATAAAAAGAGTGAGATCTCCTTATTTTTTCAATGTGAGATCCTCAACATGAACGACTTTTCAAGTGCGTAAAAAATCATTCCAAACATATGTTTGTCAAAGTATTTTTTAATCAAAATATTAGTTAAATAATGATAATAATCATCTGGCAAGAAATTACATTGTGTGAATATATATTAATCAAAATATATATTTTGAAAGTAGATGTATTGATATTTTAAGCTAAACTGAAATTTCTTTTTGTTATTTAGGATCTACTTCTTGCAGGAATGAACACTCCAGGGACCACCATCGAATGA

mRNA sequence

ATGGGTTTGATTTGGATCTTTCTTATCATCATTCATCTGCTTGTTCTTCCATTTGTCCTTTGGCCATGGCTACTGAGGAGTAACAAAACCAAGAAATTACTGCCTCCTGGTCCAAAAGGGTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGGAAGCTTCCTCACAGAGATTTTCACCGCTTGTCACAAAGGTATGGAGGCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCTTCCCCTGAAGTCGCAGAGCTGTTTCTCAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGACGTTTTCAAGCACATGTATTTTGGCCAAAACAGCATTGCGGTTGAAAAATATGGGCCTTATTTACGCAATGTGCGCAAAATATGCACTCTGGAGTTGCTTAGCAGCAAGAAACTCAATTCTTTCAAAGCAATGAGAAGGGAAGAAGTTGGGTTGTTGATTGAGGAGCTTCGTGAGGCTGCCAACAGTGGCGTGGTTGTAAATCTTACTTCTAAAGTCTCTTCTCTCAGTGCAAAGATGATATGTCTAATGATCTTTGGTAGGAAATTTGAAGACGAAGAAATTGATGAAAGGGGTTTTAAGTGGATGGTACAAGAAGCCTTCAAAGTAGTTGGAGCTTTTAACATGGGGGACTTCATTCCTTTTATTGCACCTCTTGATCTTCAAGGATTGATCCGTCGTGCAAAATCTGTTCATAAAGTATTTGATAAGTTTCTTGAAAGGATCATAGATGAACATCTTCAATCCAAGAATAATGATACCAAAGATTTCGTAGAAGTCATGTTGGACATTATGGGATCACAAGAAACCGACTACCAAATTGATCGATCCACCATCAAAGCCATCATGCTGGATCTACTTCTTGCAGGAATGAACACTCCAGGGACCACCATCGAATGA

Coding sequence (CDS)

ATGGGTTTGATTTGGATCTTTCTTATCATCATTCATCTGCTTGTTCTTCCATTTGTCCTTTGGCCATGGCTACTGAGGAGTAACAAAACCAAGAAATTACTGCCTCCTGGTCCAAAAGGGTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGGAAGCTTCCTCACAGAGATTTTCACCGCTTGTCACAAAGGTATGGAGGCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCTTCCCCTGAAGTCGCAGAGCTGTTTCTCAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGACGTTTTCAAGCACATGTATTTTGGCCAAAACAGCATTGCGGTTGAAAAATATGGGCCTTATTTACGCAATGTGCGCAAAATATGCACTCTGGAGTTGCTTAGCAGCAAGAAACTCAATTCTTTCAAAGCAATGAGAAGGGAAGAAGTTGGGTTGTTGATTGAGGAGCTTCGTGAGGCTGCCAACAGTGGCGTGGTTGTAAATCTTACTTCTAAAGTCTCTTCTCTCAGTGCAAAGATGATATGTCTAATGATCTTTGGTAGGAAATTTGAAGACGAAGAAATTGATGAAAGGGGTTTTAAGTGGATGGTACAAGAAGCCTTCAAAGTAGTTGGAGCTTTTAACATGGGGGACTTCATTCCTTTTATTGCACCTCTTGATCTTCAAGGATTGATCCGTCGTGCAAAATCTGTTCATAAAGTATTTGATAAGTTTCTTGAAAGGATCATAGATGAACATCTTCAATCCAAGAATAATGATACCAAAGATTTCGTAGAAGTCATGTTGGACATTATGGGATCACAAGAAACCGACTACCAAATTGATCGATCCACCATCAAAGCCATCATGCTGGATCTACTTCTTGCAGGAATGAACACTCCAGGGACCACCATCGAATGA

Protein sequence

MGLIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSVHKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGMNTPGTTIE

Homology

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BLAST of HG10016489 vs. NCBI nr
Match: XP_038897271.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 5.3e-135
Identity = 254/313 (81.15%), Postives = 281/313 (89.78%), Query Frame = 0


Query: 1   MGLIWIFLIIIHLLVLPFVLWPWLLR-SNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFH 60
           MGLIW+ +I IH+LV+P V W WL R +NKTKKLLPPGPKGFPIFGSLHLLGKLPHRD H
Sbjct: 1   MGLIWMIIITIHVLVVPLVFWQWLQRNNNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLH 60

Query: 61  RLSQRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAV 120
           +LSQ+YG IMH+KLGLVNTIVVSSP+ AELFLKTHDLDFANRPPN+VFKH+YFGQNSIAV
Sbjct: 61  QLSQKYGPIMHIKLGLVNTIVVSSPKAAELFLKTHDLDFANRPPNEVFKHIYFGQNSIAV 120

Query: 121 EKYGPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSS 180
             YGPYLRN+RKI TLELLSS KL+SFK MRREEVGLLIEELR AA  GVVVNLTSKVSS
Sbjct: 121 SDYGPYLRNMRKIHTLELLSSHKLDSFKPMRREEVGLLIEELRGAARRGVVVNLTSKVSS 180

Query: 181 LSAKMICLMIFGRKFEDE-EID-ERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIR 240
           L A MICLMIFGRKFED+ E+D E GFKW+VQEAFK+ GAFN+GDFIPFIAPLDLQGLIR
Sbjct: 181 LIANMICLMIFGRKFEDDKELDHEMGFKWIVQEAFKLFGAFNLGDFIPFIAPLDLQGLIR 240

Query: 241 RAKSVHKVFDKFLERIIDEHLQSKN-NDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLD 300
           RAKSVH+VFD+FLERIIDEHLQS N N T+D V++MLDIMGS++TDYQIDRSTIK I+LD
Sbjct: 241 RAKSVHQVFDRFLERIIDEHLQSNNINVTEDVVDIMLDIMGSRQTDYQIDRSTIKTIILD 300

Query: 301 LLLAGMNTPGTTI 310
           LLLAG NT  T+I
Sbjct: 301 LLLAGTNTSVTSI 313

[Close]

BLAST of HG10016489 vs. NCBI nr
Match: XP_022950431.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 428.7 bits (1101), Expect = 4.2e-116
Identity = 214/308 (69.48%), Postives = 255/308 (82.79%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  + W W+ R NK+KK LPPGPKGFPI GSLHLLGKLPHRD HRLS
Sbjct: 1   MAWIWMIII-MFALVLLFWAWMER-NKSKK-LPPGPKGFPIMGSLHLLGKLPHRDLHRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           Q YG IMHMKLGLVNTIVVSSPE AELFLKTHD+ FA+R PN+VFKH+ F Q +IA  KY
Sbjct: 61  QTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           GPY RN+RK+C LELLS+ K N  ++MRR+E+G  I +LR+AANSG+VVN++SK+ SL+A
Sbjct: 121 GPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAANSGMVVNISSKIYSLTA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMI GR+FED+E+DE GFKWM+QE F + GAFN+GDFIPFIAPLDLQG IR A+SV
Sbjct: 181 NMACLMILGRRFEDKELDEMGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFIRGARSV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           HKV D F E+I+DEHL+S NNDTKD V+VML IMGSQ T+YQID STIKAI+LD+LL G+
Sbjct: 241 HKVCDTFFEKILDEHLESNNNDTKDLVDVMLGIMGSQTTEYQIDPSTIKAIILDMLLGGV 300

Query: 303 NTPGTTIE 311
            TP TTIE
Sbjct: 301 ETPATTIE 305

[Close]

BLAST of HG10016489 vs. NCBI nr
Match: XP_022950432.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 427.9 bits (1099), Expect = 7.2e-116
Identity = 218/307 (71.01%), Postives = 258/307 (84.04%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  + WPWLL+ NK K  LPPGPKGFPI GSLHLLGKLPHRD  RLS
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLK-NKGKN-LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           QRYG IMHMKLGLVNTIVVSSPE A LFLKTHDL FA+RP N+VFKHM +G+ S+   KY
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y RN+RKICTLELLS++K+N FK+MR+EE+ LLI+ELREAA S VVVNL+SKV SLSA
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMIFG+KFED ++DERGFKW+VQEA K+  AFN+GDFIPFI+ LDLQGLI+RAK V
Sbjct: 181 NMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           +KV D+F ERI+DEHL+SKN D+KDFV+VML IMGS+ T+YQIDRS+IKAIMLD+LLA  
Sbjct: 241 NKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAAT 300

Query: 303 NTPGTTI 310
           ++   TI
Sbjct: 301 DSSAVTI 304

[Close]

BLAST of HG10016489 vs. NCBI nr
Match: XP_023545010.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423.7 bits (1088), Expect = 1.4e-114
Identity = 218/307 (71.01%), Postives = 257/307 (83.71%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  +  PW+LR NK K  LPPGPKGFPI GSLHLLGKLPHRD  RLS
Sbjct: 1   MAWIWIIII-MFALVLLFRPWVLR-NKGKN-LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           QRYG IMHMKLGLVNTIVVSSPE A LFLKTHDL +A+RP N+VFKHM +G+ S+   KY
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVYASRPTNEVFKHMSYGERSMVFSKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y RN+RKICTLELLS++K+N FK+MRREE+ LLI+ELREAA S VVVNL+SKV SLSA
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMIFG+KFED E+DERGFKW+VQEA K+  AFN+GDFIPFI+ LDLQGLI+RAK V
Sbjct: 181 NMTCLMIFGKKFEDRELDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           +KV D+F ERI+DEHL+SKN D+KDFV+VML IMGS+ T+YQIDRS+IKAIMLD+LLA  
Sbjct: 241 NKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAAT 300

Query: 303 NTPGTTI 310
           ++   TI
Sbjct: 301 DSSAVTI 304

[Close]

BLAST of HG10016489 vs. NCBI nr
Match: XP_022978479.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 421.0 bits (1081), Expect = 8.8e-114
Identity = 217/307 (70.68%), Postives = 256/307 (83.39%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  +   WLL+ NK K  LPPGPKGFPI GSLHLLGKLPHRD  RLS
Sbjct: 1   MAWIWMIII-MFALVLLFRSWLLK-NKAKN-LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           QRYG IMHMKLGLVNTIVVSSPE A LFLKTHDL FA+RP N VFKHM +G+ S+   KY
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y RN+RKICTLELLS++K+N FK+MRREE+ LLI+ELREAA S VVVNL+SKV SLSA
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMIFG+KFED ++DERGFKW+VQEA K+  AFN+GDFIPFI+ LDLQGLI+RAK V
Sbjct: 181 NMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           +KV D+F ERI+DEHL+SKN+D+KDFV+VML IMGS+ T+YQIDRS+IKAIMLD+LLA  
Sbjct: 241 NKVCDRFFERILDEHLESKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAAT 300

Query: 303 NTPGTTI 310
           ++   TI
Sbjct: 301 DSSAVTI 304

[Close]

BLAST of HG10016489 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.9e-95
Identity = 181/310 (58.39%), Postives = 239/310 (77.10%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           ++WI+   I LL L  +L  W    NK KK LPPGP+GFPIFGSLHLLG+ P++D HRL+
Sbjct: 1   MVWIW-ATIGLLALVHILQAWW--KNK-KKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLA 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           ++YG IM+M+LGL+ TIV+SSPE AELFLKTHDL FA+RPP++  KH+ FGQ ++   +Y
Sbjct: 61  RKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANS-GVVVNLTSKVSSLS 182
           G Y R+ RK+CT+ELLS+ K+NSFK+MRREEV L +E +R AAN+ GV V+L+ KVSSLS
Sbjct: 121 GAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLS 180

Query: 183 AKMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKS 242
             M C M+ G+K+ DEE DERGFK +V+EA ++  A N+GD+I FIAPLDLQG  +R KS
Sbjct: 181 VDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKS 240

Query: 243 VHKVFDKFLERIIDEHLQSKNND-TKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLA 302
           V+K FD   E+II+EHLQ  + + T DFV+VM+  MGS+E++Y+I+R  IKAIMLD+L+A
Sbjct: 241 VNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVA 300

Query: 303 GMNTPGTTIE 311
            M+T  TTIE
Sbjct: 301 SMDTSATTIE 306

[Close]

BLAST of HG10016489 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 138/315 (43.81%), Postives = 198/315 (62.86%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           L+++ L  +   +LP      ++   K    LPPGP+G PI GSLH LG LPHR    L+
Sbjct: 8   LLFVLLGALSWWILP------IISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLA 67

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           ++YG IM M+LG V TIVVSSP+ AELFLKTHD  FA+RP     ++M +G   ++   Y
Sbjct: 68  KKYGPIMSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAY 127

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           GP+ RN+RK   LELL+  K+NSF  MRREE+G +++ ++EA+ +  VV+L++KV+++  
Sbjct: 128 GPHWRNIRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIE 187

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M   ++ GR  +D+  D +G   ++ EA  + G FN+ DF+PF+ PLD+QGL R+ K  
Sbjct: 188 NMTYRLLLGRT-KDDRYDLKG---IMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDT 247

Query: 243 HKVFDKFLERIIDEHLQ--SKNNDTKDFVEVMLDIMGS-----QETDYQIDRSTIKAIML 302
            K  DK LE IIDEH Q  S  N + DF++ ML +         E    IDRS IKAIM+
Sbjct: 248 GKRLDKILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMI 307

Query: 303 DLLLAGMNTPGTTIE 311
           D++ A ++T  T+IE
Sbjct: 308 DIISAAIDTSDTSIE 312

[Close]

BLAST of HG10016489 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 8.7e-56
Identity = 119/312 (38.14%), Postives = 189/312 (60.58%), Query Frame = 0


Query: 13  LLVLPFVLW--PWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRYGGIMH 72
           +L+  F+ +   W+L  ++  + LPPGP  +PI G+ H +    HR    L+++YG I+ 
Sbjct: 19  ILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILF 78

Query: 73  MKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPYLRNVR 132
           ++ G V T+VVSS E A+ FLKTHDL FA+RPP  V K+ ++    IA   YG + R +R
Sbjct: 79  LRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMR 138

Query: 133 KICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSG-VVVNLTSKVSSLSAKMICLMI 192
           KIC LELL+SK++ SFK +R+EE+  +I  + E + SG + VN++  +S+  A ++  ++
Sbjct: 139 KICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRIL 198

Query: 193 FGRKFEDEEI--DERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSVHKVFD 252
             +KF D ++  D +GF  +V E    VG+ N+GDFIP++  LDLQG+ R  K  +  FD
Sbjct: 199 ARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFD 258

Query: 253 KFLERIIDEHLQS---KNND------TKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLL 311
            F E++IDEH+ +   +N +       KD ++V+L++  +  T  ++ R  IKAI  +L 
Sbjct: 259 AFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELF 318

[Close]

BLAST of HG10016489 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-54
Identity = 118/312 (37.82%), Postives = 184/312 (58.97%), Query Frame = 0


Query: 9   IIIHLLVLPFVLWPWLLRSN---KTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRY 68
           I++ LL L   L  +LL+ N   + K  LPP P   PI G+LH LG LPHR    L+   
Sbjct: 3   ILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANEL 62

Query: 69  GGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPY 128
           G ++ + LG + T++VS+ E+AE  LKTHDL FA+RP     + +++    +A   YG Y
Sbjct: 63  GPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEY 122

Query: 129 LRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMI 188
            R VRKIC LELLS K++NS++++R EEVGL++E + ++ ++G  VNL+  +  LS+  I
Sbjct: 123 WRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTI 182

Query: 189 CLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLD-LQGLIRRAKSVHK 248
             + FG+K+E EE  +  F  +  E   ++GAF +GD+ P  A +D L G+  R K  H 
Sbjct: 183 TRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHG 242

Query: 249 VFDKFLERIIDEHLQSKNN------DTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLL 308
             D F++ +ID+HL S+        + KD V+V+L +         ++R+ +KA++LD+ 
Sbjct: 243 ELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMF 302

Query: 309 LAGMNTPGTTIE 311
             G +T   T+E
Sbjct: 303 SGGTDTTAVTLE 314

[Close]

BLAST of HG10016489 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.5e-52
Identity = 117/315 (37.14%), Postives = 189/315 (60.00%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           L + FL I  LL+  F+++    R +     LPPGP G+PI G+L  LG  PH+    LS
Sbjct: 2   LTFFFLWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALS 61

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           ++YG +  ++LG VN +V SS  VA  FL+THD++F+NRPPN   +H+ +    +    Y
Sbjct: 62  KQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPY 121

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           GP  R +RK+C++ L S K L+  + +R+ EV  L+  LR  A++GV+VNL   ++  + 
Sbjct: 122 GPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCAT 181

Query: 183 KMICLMIFGRK-FEDE-----EIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLI 242
             +   + GR+ F DE     E DE  FK MV E  ++ G FN+GDF+P +  LDLQG++
Sbjct: 182 NALARAMLGRRVFADEDAQLAEADE--FKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 241

Query: 243 RRAKSVHKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIM-GSQETDYQIDRSTIKAIML 302
            + K +H+ +D FL+R+I+E+    N  + D + V++ +     E + +++ + IKA++L
Sbjct: 242 GKMKRLHRRYDAFLDRVIEEN--QANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLL 301

Query: 303 DLLLAGMNTPGTTIE 311
           +L  AG +T  +T+E
Sbjct: 302 NLFTAGTDTSSSTVE 312

[Close]

BLAST of HG10016489 vs. ExPASy TrEMBL
Match: A0A6J1GET5 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 2.1e-116
Identity = 214/308 (69.48%), Postives = 255/308 (82.79%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  + W W+ R NK+KK LPPGPKGFPI GSLHLLGKLPHRD HRLS
Sbjct: 1   MAWIWMIII-MFALVLLFWAWMER-NKSKK-LPPGPKGFPIMGSLHLLGKLPHRDLHRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           Q YG IMHMKLGLVNTIVVSSPE AELFLKTHD+ FA+R PN+VFKH+ F Q +IA  KY
Sbjct: 61  QTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           GPY RN+RK+C LELLS+ K N  ++MRR+E+G  I +LR+AANSG+VVN++SK+ SL+A
Sbjct: 121 GPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAANSGMVVNISSKIYSLTA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMI GR+FED+E+DE GFKWM+QE F + GAFN+GDFIPFIAPLDLQG IR A+SV
Sbjct: 181 NMACLMILGRRFEDKELDEMGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFIRGARSV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           HKV D F E+I+DEHL+S NNDTKD V+VML IMGSQ T+YQID STIKAI+LD+LL G+
Sbjct: 241 HKVCDTFFEKILDEHLESNNNDTKDLVDVMLGIMGSQTTEYQIDPSTIKAIILDMLLGGV 300

Query: 303 NTPGTTIE 311
            TP TTIE
Sbjct: 301 ETPATTIE 305

[Close]

BLAST of HG10016489 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.5e-116
Identity = 218/307 (71.01%), Postives = 258/307 (84.04%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  + WPWLL+ NK K  LPPGPKGFPI GSLHLLGKLPHRD  RLS
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLK-NKGKN-LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           QRYG IMHMKLGLVNTIVVSSPE A LFLKTHDL FA+RP N+VFKHM +G+ S+   KY
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y RN+RKICTLELLS++K+N FK+MR+EE+ LLI+ELREAA S VVVNL+SKV SLSA
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMIFG+KFED ++DERGFKW+VQEA K+  AFN+GDFIPFI+ LDLQGLI+RAK V
Sbjct: 181 NMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           +KV D+F ERI+DEHL+SKN D+KDFV+VML IMGS+ T+YQIDRS+IKAIMLD+LLA  
Sbjct: 241 NKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAAT 300

Query: 303 NTPGTTI 310
           ++   TI
Sbjct: 301 DSSAVTI 304

[Close]

BLAST of HG10016489 vs. ExPASy TrEMBL
Match: A0A6J1IU54 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 4.3e-114
Identity = 217/307 (70.68%), Postives = 256/307 (83.39%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  +   WLL+ NK K  LPPGPKGFPI GSLHLLGKLPHRD  RLS
Sbjct: 1   MAWIWMIII-MFALVLLFRSWLLK-NKAKN-LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           QRYG IMHMKLGLVNTIVVSSPE A LFLKTHDL FA+RP N VFKHM +G+ S+   KY
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y RN+RKICTLELLS++K+N FK+MRREE+ LLI+ELREAA S VVVNL+SKV SLSA
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSA 180

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSV 242
            M CLMIFG+KFED ++DERGFKW+VQEA K+  AFN+GDFIPFI+ LDLQGLI+RAK V
Sbjct: 181 NMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCV 240

Query: 243 HKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGM 302
           +KV D+F ERI+DEHL+SKN+D+KDFV+VML IMGS+ T+YQIDRS+IKAIMLD+LLA  
Sbjct: 241 NKVCDRFFERILDEHLESKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAAT 300

Query: 303 NTPGTTI 310
           ++   TI
Sbjct: 301 DSSAVTI 304

[Close]

BLAST of HG10016489 vs. ExPASy TrEMBL
Match: A0A6J1GEV6 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.2e-109
Identity = 207/308 (67.21%), Postives = 248/308 (80.52%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           + WI++III +  L  + W WL R+   K  LPPGPKGFPI GSLHLLGKLPHRD HRLS
Sbjct: 1   MAWIWIIII-MFTLVLLFWAWLGRNQSQK--LPPGPKGFPILGSLHLLGKLPHRDLHRLS 60

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
           Q YG IMHMKLGLVNTIVVSSPE AELFLKTHDL FA+R PN+VFKH+ FGQ ++   KY
Sbjct: 61  QTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRAPNEVFKHISFGQKTMVFAKY 120

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELR-EAANSGVVVNLTSKVSSLS 182
           GPY RN+ K+C LELLS+ K++SFK+MRR+E+G+LI++LR +AANS VVVNLTSK+ SL+
Sbjct: 121 GPYWRNMHKMCVLELLSNHKIDSFKSMRRQELGVLIDQLRDDAANSRVVVNLTSKIYSLT 180

Query: 183 AKMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKS 242
             M CLM+ GRKFED+EIDERGFKWM+QE F + GAFN+GDFIPFIAPLDLQG   RA+S
Sbjct: 181 INMACLMVLGRKFEDKEIDERGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFTPRARS 240

Query: 243 VHKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAG 302
           VHKV D   E+I+DEHL+S +N TKDFV+VML IMGSQ+     DRS IKAIMLD+LL  
Sbjct: 241 VHKVCDTLFEKILDEHLESNHNHTKDFVDVMLGIMGSQQ-----DRSNIKAIMLDMLLGA 300

Query: 303 MNTPGTTI 310
           ++T  T+I
Sbjct: 301 VDTATTSI 300

[Close]

BLAST of HG10016489 vs. ExPASy TrEMBL
Match: A0A6J1BSN9 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.9e-106
Identity = 203/306 (66.34%), Postives = 242/306 (79.08%), Query Frame = 0


Query: 6   IFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRY 65
           I+++I HLL L  VL PWL   N  K  LPPGPKGFPI G LHL GK PHRD HRLSQ Y
Sbjct: 4   IWILIFHLLALALVLRPWLYLRNSHKNKLPPGPKGFPILGCLHLFGKNPHRDLHRLSQTY 63

Query: 66  GGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPY 125
           G IMH+KLGLV+TIVVSSP  AELFLKT+DL FANRPP++VF H+ +G  +I    YG Y
Sbjct: 64  GPIMHIKLGLVHTIVVSSPRAAELFLKTYDLVFANRPPSEVFTHIAYGLKNILFAPYGSY 123

Query: 126 LRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMI 185
             N+RK+CTLELL+S K+ SFK+MRREEVGLL E+LREAA +GV V+LTSK+ SL+  M 
Sbjct: 124 WSNMRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNLDMT 183

Query: 186 CLMIFGRKFEDEEI-DERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSVHK 245
           CLMIFGRK+  +E+ DERGFKW+VQEA ++  A N+GD IP IAP DLQGL RRAKSV K
Sbjct: 184 CLMIFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKSVGK 243

Query: 246 VFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGMNT 305
           V D FLE IIDEHL+  +N+TKDFV+VMLDI+GS+ET+YQIDRS IKAI+LD++LA  +T
Sbjct: 244 VLDGFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLATTDT 303

Query: 306 PGTTIE 311
             TTIE
Sbjct: 304 TATTIE 309

[Close]

BLAST of HG10016489 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 195.3 bits (495), Expect = 7.4e-50
Identity = 112/310 (36.13%), Postives = 180/310 (58.06%), Query Frame = 0


Query: 8   LIIIHLLVLPFVLWPWLLRSNKTKKL-LPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRYG 67
           L+   L+ L  ++    L+ NKT K  LPP P   PI G+LH L  LPHR FH+LS +YG
Sbjct: 4   LLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYG 63

Query: 68  GIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPYL 127
            ++ ++LG V  +V+SS E AE  LKT+DL+  +RP       + +G   I    YG Y 
Sbjct: 64  PLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYW 123

Query: 128 RNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMIC 187
           R VRK+  +EL SSKK+ SF+ +R EEV  +++++ E+A     V+L+    SL+A +IC
Sbjct: 124 REVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIIC 183

Query: 188 LMIFGRKFEDE--EIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLDLQGLIRRAKSVHK 247
            +  G+ F +    ID+   + +V E+ + +G F   DF P      +  L +R K ++K
Sbjct: 184 RVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINK 243

Query: 248 VF---DKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQE--TDYQIDRSTIKAIMLDLLL 307
           VF   D F + +ID+HL+ +    +D V ++LD++  QE    ++++   +KAI++D+ L
Sbjct: 244 VFKELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFL 303

Query: 308 AGMNTPGTTI 310
           AG++T   T+
Sbjct: 304 AGIDTSAVTM 313

[Close]

BLAST of HG10016489 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 192.2 bits (487), Expect = 6.2e-49
Identity = 107/309 (34.63%), Postives = 186/309 (60.19%), Query Frame = 0


Query: 3   LIWIFLIIIHLLVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLS 62
           +I  FL+   + V+  +++    R  K   L  P P G P+ G+LH LG+ PHR    LS
Sbjct: 2   MIMFFLLCSIIFVVTIIIFRKQKRGKKRNTL--PSPPGLPLIGNLHQLGRHPHRSLCSLS 61

Query: 63  QRYGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKY 122
            RYG +M +  G V  +VVSS E+A   LKTHD  FA+RP + +F+ + + ++ +A   Y
Sbjct: 62  HRYGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPY 121

Query: 123 GPYLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSA 182
           G Y R ++ +C L L S+K + SF+ +R EE+ L++E++R++ +  + VNL+  + SL+ 
Sbjct: 122 GEYWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSIS--LPVNLSKILVSLTN 181

Query: 183 KMICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLD-LQGLIRRAKS 242
            +IC +  GRK+      E  FK +++   K++G F++G ++P++A +D ++GL  + + 
Sbjct: 182 DVICKVALGRKYG----GETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEK 241

Query: 243 VHKVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAG 302
                DKF ER++ +H+   N D  DFV+V+L I   +   ++I+R +IKAI++++ + G
Sbjct: 242 TANDVDKFFERVVQDHVDG-NRDMTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGG 301

Query: 303 MNTPGTTIE 311
            +T  T +E
Sbjct: 302 TDTSSTLME 301

[Close]

BLAST of HG10016489 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-48
Identity = 106/303 (34.98%), Postives = 186/303 (61.39%), Query Frame = 0


Query: 10  IIHLLVLPFVLWPWLLRSNKTKKL-LPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRYGGI 69
           II L ++ F+   + ++  K KK   P  P   P+ G+LH LG+ PHR    LS RYG +
Sbjct: 7   IILLSLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPL 66

Query: 70  MHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPYLRN 129
           M ++ GLV  +VVSS +VA   LKT+D  FA+RP + +F+ +++    +A+  YG Y R 
Sbjct: 67  MLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQ 126

Query: 130 VRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMICLM 189
           ++ +C L LL++K + SF+ +R+EE+ L++E++++  +S + VNL+  + SL+  +I  +
Sbjct: 127 MKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQK--SSSLQVNLSELLGSLTNDVISRV 186

Query: 190 IFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLD-LQGLIRRAKSVHKVFD 249
             GRK+     DE  FK +++   K++G F +G ++P++A +D + GL  + K      D
Sbjct: 187 ALGRKYS----DETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLD 246

Query: 250 KFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGMNTPGT 309
           +FLE+++ +H +  +    DFV+V+L I   +   ++IDR +IKAI+LD+++ G +T   
Sbjct: 247 EFLEKVVQDH-EDGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYA 302

Query: 310 TIE 311
            +E
Sbjct: 307 LME 302

[Close]

BLAST of HG10016489 vs. TAIR 10
Match: AT5G24950.1 (cytochrome P450, family 71, subfamily A, polypeptide 15 )

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-48
Identity = 107/307 (34.85%), Postives = 173/307 (56.35%), Query Frame = 0


Query: 8   LIIIHL---LVLPFVLWPWLLRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQR 67
           +III L    +L F+L   LL     K  LPP P   P+ G+LH L   PHR    LS R
Sbjct: 3   IIIISLCLATILAFLLLKPLLNRTVAKDNLPPSPWRVPVIGNLHQLSLHPHRSLRSLSHR 62

Query: 68  YGGIMHMKLGLVNTIVVSSPEVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGP 127
           YG +M +  G V  +VVSS +VA   +KTHDL  ANRP   V + +  G   +    YG 
Sbjct: 63  YGPLMLLHFGRVPILVVSSSDVAHDLMKTHDLKVANRPRLKVIETILNGGREVVFSPYGD 122

Query: 128 YLRNVRKICTLELLSSKKLNSFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKM 187
           Y R ++ +C + LL+ K + SF  +R EE  +++E++ +A++    +NL+  + +L++ +
Sbjct: 123 YWRQIKTVCVVHLLNKKMVQSFAKVREEERSVMMEKVEKASSDSSPLNLSKLLITLTSDV 182

Query: 188 ICLMIFGRKFEDEEIDERGFKWMVQEAFKVVGAFNMGDFIPFIAPLD-LQGLIRRAKSVH 247
              + FG+K    E     FK  V++  ++VG F + ++IP +A +D ++GL  RA+ V 
Sbjct: 183 ASRVSFGKK-HSNEASMSDFKNQVRKITELVGGFPVSEYIPCLAWIDQIRGLYNRAEEVS 242

Query: 248 KVFDKFLERIIDEHLQSKNNDTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGMN 307
           K+F   +++++ EHL + N  TKDFV+++L      +   ++ RS IK I+LD+ L G  
Sbjct: 243 KIFGDLMDKVVQEHLDATNKPTKDFVDILLSFERQSKDGIEVRRSDIKFIILDIFLGGTT 302

Query: 308 TPGTTIE 311
           T  + +E
Sbjct: 303 TTNSLLE 308

[Close]

BLAST of HG10016489 vs. TAIR 10
Match: AT2G30770.1 (cytochrome P450, family 71, subfamily A, polypeptide 13 )

HSP 1 Score: 189.9 bits (481), Expect = 3.1e-48
Identity = 99/287 (34.49%), Postives = 167/287 (58.19%), Query Frame = 0


Query: 25  LRSNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDFHRLSQRYGGIMHMKLGLVNTIVVSSP 84
           L+   TK  LPP P   P+ G+LH L   PHR    LS RYG +M +  G V  +VVSS 
Sbjct: 30  LKRTATKVNLPPSPWRLPVIGNLHQLSLHPHRSLRSLSLRYGPLMLLHFGRVPILVVSSG 89

Query: 85  EVAELFLKTHDLDFANRPPNDVFKHMYFGQNSIAVEKYGPYLRNVRKICTLELLSSKKLN 144
           E A+  LKTHD  FANRP +     +  G   +    YG Y R ++ +C L LL++K + 
Sbjct: 90  EAAQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVE 149

Query: 145 SFKAMRREEVGLLIEELREAANSGVVVNLTSKVSSLSAKMICLMIFGRKFEDEEIDERGF 204
           SF+ +R +EV  +IE+L +A++S    NL+    +L + +   +  GRK  ++E   R  
Sbjct: 150 SFEKVREDEVNAMIEKLEKASSSSSSENLSELFITLPSDVTSRVALGRKHSEDE-TARDL 209

Query: 205 KWMVQEAFKVVGAFNMGDFIPFIAPLD-LQGLIRRAKSVHKVFDKFLERIIDEHLQSKNN 264
           K  V++  +++G F +G+++P +A +D ++G   + K V + F   +++++ EHL++ +N
Sbjct: 210 KKRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSDLMDKVVQEHLEA-SN 269

Query: 265 DTKDFVEVMLDIMGSQETDYQIDRSTIKAIMLDLLLAGMNTPGTTIE 311
           D  DFV+++L I   + + +Q+ R+ IK ++LD+ + G +T  T +E
Sbjct: 270 DKADFVDILLSIEKDKNSGFQVQRNDIKFMILDMFIGGTSTTSTLLE 314

The following BLAST results are available for this feature:

BLAST of HG10016489 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897271.1	5.3e-135	81.15	cytochrome P450 71AU50-like [Benincasa hispida]	[more]
XP_022950431.1	4.2e-116	69.48	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]
XP_022950432.1	7.2e-116	71.01	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]
XP_023545010.1	1.4e-114	71.01	cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978479.1	8.8e-114	70.68	cytochrome P450 CYP736A12-like [Cucurbita maxima]	[more]

BLAST of HG10016489 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q5V6	1.9e-95	58.39	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
H2DH18	1.9e-63	43.81	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
Q50EK4	8.7e-56	38.14	Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1	[more]
P24465	1.3e-54	37.82	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A4D6Q415	4.5e-52	37.14	Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN...	[more]

BLAST of HG10016489 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GET5	2.1e-116	69.48	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=...	[more]
A0A6J1GET0	3.5e-116	71.01	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=...	[more]
A0A6J1IU54	4.3e-114	70.68	cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 ...	[more]
A0A6J1GEV6	3.2e-109	67.21	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=...	[more]
A0A6J1BSN9	1.9e-106	66.34	cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE...	[more]

BLAST of HG10016489 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13080.1	7.4e-50	36.13	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT3G48270.1	6.2e-49	34.63	cytochrome P450, family 71, subfamily A, polypeptide 26	[more]
AT3G48310.1	1.1e-48	34.98	cytochrome P450, family 71, subfamily A, polypeptide 22	[more]
AT5G24950.1	2.4e-48	34.85	cytochrome P450, family 71, subfamily A, polypeptide 15	[more]
AT2G30770.1	3.1e-48	34.49	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 62..81 score: 29.01 coord: 86..107 score: 25.53 coord: 289..306 score: 30.83 coord: 180..198 score: 26.38
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 35..309 e-value: 9.4E-39 score: 133.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..310 e-value: 4.3E-59 score: 202.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..309
None	No IPR available	PANTHER	PTHR47943	CYTOCHROME P450 93A3-LIKE	coord: 6..310
None	No IPR available	PANTHER	PTHR47943:SF2	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 6..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016489.1	HG10016489.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen