HG10016333 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016333
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr03: 4255167 .. 4257384 (+)
RNA-Seq Expression	HG10016333
Synteny	HG10016333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAACACAGGCAATCTCGTTTCCCTACTCGGAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACCTTCGTCATTTTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGTTCGATTGTGCGGAAAACTTCCGACGAGTATGTGATCTCGTGGGTTCGCTTTCAATTTTTGGTTCTGTTTATAGGGTTGGTTTTTGATTGATTATGTGGTTCTGTTTGATTTTCGTTTCAGTGTTGATGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCCTTTGTTTATCACAATTTTCTGGTATTCTTTTCTTCCCGATCTGTAATGAGAGAGATTTGTGTTTTAGGTTAATTCATCTCTCTATGCAATTTGTGTATGGATTTCTTTAAACTTGGTTGGTGTATGCTTATACATGTTGAATGCGTTTTTGGGGCTGCACGTGTTAATCAAAACCAAGGGCATTTTTGAAATGAAGTCTTTTGGGGGCTGCACATCCCTTAAGTTGATTATGTTTATGTTCCTCTAAGAAGTCATTCTTCTTCTGTGTTATTATGATCAGAGGGGTTAAGGATAATTCCCATCTTTATATGTCTTTAGATTGTGTTGGAGTTTTGAGTTGACTTAGGCTGAGGCCCATCAATTGATCTCGGAGTAGGGAAATACTGAATTGGTTTACTTTTTAATTTCCCAGACAAAGGAGGAATGTGAATACCTAATCAGCCTGGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGATAGTGAAACTGGAAAGAGCAAAGATAGCAGGTGTGCTATTCATTTGCTCACAATTCTTTATTGTTAGTATTGCTGGATCTATAATGTGGAAACCTCCCTGACCTCTTTTTCACAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTATCAGAACTATAGAGAAAAGGATTGCTGATTTCTCCTTCATACCCGTAGGTACATTTTTTGAGCCTATTTCCTTCTTCCTGGTTTCTTTCCACTACATCAAGCATTATAGTAATATAGTTTGATTGTCCATGGTTAAAATTGAACTTTGTAATCTTTAATTTTTTTTCCAAGTGCACTTATCATTTTGGTAAAAATTGTCCACCTTAACCTAGCATTGCCAATCATAAATTTTCTTTTGATAGAAATAGATTATCACAATCATGCCCGAGACCATTCCAATAGACACAATGATCATGCTAAGTTTCACATGGATGAGGAAGATTTTTAACCATGGCTGAAAATTTGAATAACGAATATCAACTCAGATTTTGTCCTATGGGCTATGAGCTTGCTTACCTATTGTTCCTTGTAAGAATGAAAGATAATTGGGTTAACCTGATAAGCTGTGCCAGTTCTTTTTTAATTATGATGTATATGTAGATTTTGTCTTCTTATCTTCTAGATTTTTCATAAGATCAATAATTACTAGACACTTTTTTCTCTCTCCATATTTTGTTCCTTAAGTGCACACATAATAATATATGCAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTGTAAGTAGCAATCCATATTTACTATGCTTTGAGCTCGGAACAAGTGCTATTACATCTTGCATGGTTCTCCCATTATTTTATATGACTTCTATCATAATTCAGCTCAGAAGTCGAAGAAGGAGGCGAGACAGTGTTCCCTGCAGCCAAAGGAAATATTAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGGCTTTCTGTTAAACCGAAGAGGGGTGATGCGTTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCTTCAAGTTTGCATGGTTAGATTAACCCACATTCATCTTACTGAGCTTGATAGGTTTCAGTGACGAATGTTCATTTTTGTCAGTGCGATTTCATTTGCATCTCTTATTCAGAAAATAATTGATAAACCGATGACATTTCTTCTGCTGTTCATTTATAGGTGGCTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

mRNA sequence

ATGGCGAAACACAGGCAATCTCGTTTCCCTACTCGGAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACCTTCGTCATTTTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGTTCGATTGTGCGGAAAACTTCCGACGATGTTGATGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCCTTTGTTTATCACAATTTTCTGACAAAGGAGGAATGTGAATACCTAATCAGCCTGGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGATAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTATCAGAACTATAGAGAAAAGGATTGCTGATTTCTCCTTCATACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTCTCAGAAGTCGAAGAAGGAGGCGAGACAGTGTTCCCTGCAGCCAAAGGAAATATTAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGGCTTTCTGTTAAACCGAAGAGGGGTGATGCGTTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCTTCAAGTTTGCATGGTGGCTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

Coding sequence (CDS)

ATGGCGAAACACAGGCAATCTCGTTTCCCTACTCGGAAGTCCTCTTCTTCCTCTACTCTCGTCTTTACCTTGCTCATTATGTTCACCTTCGTCATTTTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGGAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGTTCGATTGTGCGGAAAACTTCCGACGATGTTGATGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCATGGGAACCTAGAGCCTTTGTTTATCACAATTTTCTGACAAAGGAGGAATGTGAATACCTAATCAGCCTGGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGATAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGCTCTGGAACGTTTTTGCCGAGAGGACGTGATAAGACTATCAGAACTATAGAGAAAAGGATTGCTGATTTCTCCTTCATACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAGTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTCTCAGAAGTCGAAGAAGGAGGCGAGACAGTGTTCCCTGCAGCCAAAGGAAATATTAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGGCTTTCTGTTAAACCGAAGAGGGGTGATGCGTTGCTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCTTCAAGTTTGCATGGTGGCTGCCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

Protein sequence

MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA

Homology

[Close]

BLAST of HG10016333 vs. NCBI nr
Match: XP_038881910.1 (probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 575.9 bits (1483), Expect = 2.0e-160
Identity = 283/286 (98.95%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDDVDEE+GEQWVEVISWEPRAFVYHNFLTKEECEYLISLA PHMQKSTVVDSETGK
Sbjct: 61  RKTSDDVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGN SSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286

[Close]

BLAST of HG10016333 vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 573.2 bits (1476), Expect = 1.3e-159
Identity = 280/287 (97.56%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIRTIEKRI+DFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of HG10016333 vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 2.2e-159
Identity = 279/287 (97.21%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of HG10016333 vs. NCBI nr
Match: XP_038881909.1 (probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 568.5 bits (1464), Expect = 3.2e-158
Identity = 282/290 (97.24%), Postives = 284/290 (97.93%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDD----VDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDS 120
           RKTSD+    VDEE+GEQWVEVISWEPRAFVYHNFLTKEECEYLISLA PHMQKSTVVDS
Sbjct: 61  RKTSDEYVISVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDS 120

Query: 121 ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP 180
           ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP
Sbjct: 121 ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP 180

Query: 181 HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGL 240
           HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGN SSVPWWNELSECGKKGL
Sbjct: 181 HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGL 240

Query: 241 SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 290

[Close]

BLAST of HG10016333 vs. NCBI nr
Match: XP_022133302.1 (probable prolyl 4-hydroxylase 10 [Momordica charantia])

HSP 1 Score: 561.6 bits (1446), Expect = 3.9e-156
Identity = 273/287 (95.12%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAFVYHNFLTKEECEYL+SLAKP+MQKSTVVDSETGK
Sbjct: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIR IEKRIADFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 287

[Close]

BLAST of HG10016333 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.6e-131
Identity = 229/288 (79.51%), Postives = 254/288 (88.19%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+DEYNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of HG10016333 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.1e-120
Identity = 209/281 (74.38%), Postives = 242/281 (86.12%), Query Frame = 0


Query: 8   RFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGKSKDSR 127
             +E  GE+WVEVISWEPRA VYHNFLT EECE+LISLAKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+ +  IEKRI+DF+FIPVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLS+V++GGETVFPAA+GNIS+VPWWNELS+CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of HG10016333 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.3e-119
Identity = 211/288 (73.26%), Postives = 246/288 (85.42%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RGRDK I+TIEKRIAD++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+DE+NTKNGGQR+AT+LMYLS+VEEGGETVFPAA  N SSVPW+NELSECGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of HG10016333 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.3e-111
Identity = 198/288 (68.75%), Postives = 234/288 (81.25%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSE 122
              R++  D ++  G++W+EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+ +  IE RI+DF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLS 242
            DYF DE+N + GGQRIATVLMYLS+V+EGGETVFPAAKGN+S VPWW+ELS+CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of HG10016333 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-64
Identity = 120/231 (51.95%), Postives = 171/231 (74.03%), Query Frame = 0


Query: 57  SSIVRKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDS 116
           +S++  +S  V+  K    V+ +S +PRAFVY  FLT+ EC++++SLAK  +++S V D+
Sbjct: 22  TSLISSSSVFVNPSK----VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADN 81

Query: 117 ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP 176
           ++G+SK S VRTSSGTF+ +G+D  +  IE +I+ ++F+P E+GE +QVL YE GQKY+ 
Sbjct: 82  DSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDA 141

Query: 177 HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWN--ELSECGKK 236
           HFDYF D+ N   GG R+AT+LMYLS V +GGETVFP A+     V   N  +LS+C K+
Sbjct: 142 HFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKR 201

Query: 237 GLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           G++VKP++GDALLF+++ PDA  DP SLHGGCPVI+G KWSATKW+ V+ +
Sbjct: 202 GIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSF 248

[Close]

BLAST of HG10016333 vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.2e-160
Identity = 280/287 (97.56%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDDVDEEKGEQWVEVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIRTIEKRI+DFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of HG10016333 vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.1e-159
Identity = 279/287 (97.21%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+RTIEKR++DFSFIPVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDEYNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of HG10016333 vs. ExPASy TrEMBL
Match: A0A6J1BWA6 (probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 273/287 (95.12%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAFVYHNFLTKEECEYL+SLAKP+MQKSTVVDSETGK
Sbjct: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIR IEKRIADFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 287

[Close]

BLAST of HG10016333 vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 7.1e-156
Identity = 271/287 (94.43%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK I TIEKRIADF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of HG10016333 vs. ExPASy TrEMBL
Match: A0A6J1GDV6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.1e-155
Identity = 271/287 (94.43%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSR PTRKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK I TIEKRIADF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of HG10016333 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 469.5 bits (1207), Expect = 1.9e-132
Identity = 229/288 (79.51%), Postives = 254/288 (88.19%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+DEYNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of HG10016333 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.3e-121
Identity = 209/281 (74.38%), Postives = 242/281 (86.12%), Query Frame = 0


Query: 8   RFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGKSKDSR 127
             +E  GE+WVEVISWEPRA VYHNFLT EECE+LISLAKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+ +  IEKRI+DF+FIPVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLS+V++GGETVFPAA+GNIS+VPWWNELS+CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of HG10016333 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 429.9 bits (1104), Expect = 1.6e-120
Identity = 211/288 (73.26%), Postives = 246/288 (85.42%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDVD--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           R  ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RGRDK I+TIEKRIAD++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+DE+NTKNGGQR+AT+LMYLS+VEEGGETVFPAA  N SSVPW+NELSECGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of HG10016333 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 1.6e-112
Identity = 198/288 (68.75%), Postives = 234/288 (81.25%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSE 122
              R++  D ++  G++W+EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+ +  IE RI+DF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLS 242
            DYF DE+N + GGQRIATVLMYLS+V+EGGETVFPAAKGN+S VPWW+ELS+CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of HG10016333 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.2e-100
Identity = 183/237 (77.22%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS STLVF +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSET 120
           RKT      D+ K E+WVE+ISWEPRA VYHNFL  EEC+YLI LAKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIPVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKG 236
           DYF+DEYNT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK G
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of HG10016333 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881910.1	2.0e-160	98.95	probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida]	[more]
XP_008440878.1	1.3e-159	97.56	PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo]	[more]
XP_004134841.1	2.2e-159	97.21	probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol...	[more]
XP_038881909.1	3.2e-158	97.24	probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida]	[more]
XP_022133302.1	3.9e-156	95.12	probable prolyl 4-hydroxylase 10 [Momordica charantia]	[more]

BLAST of HG10016333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	2.6e-131	79.51	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q24JN5	6.1e-120	74.38	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
Q9LN20	2.3e-119	73.26	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JNU8	2.3e-111	68.75	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q8LAN3	1.6e-64	51.95	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of HG10016333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B1P6	6.2e-160	97.56	probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV...	[more]
A0A0A0KJH9	1.1e-159	97.21	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1BWA6	1.9e-156	95.12	probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 ...	[more]
A0A6J1IRF1	7.1e-156	94.43	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=...	[more]
A0A6J1GDV6	2.1e-155	94.43	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 P...	[more]

BLAST of HG10016333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	1.9e-132	79.51	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	4.3e-121	74.38	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	1.6e-120	73.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	1.6e-112	68.75	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	3.2e-100	77.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 83..281 e-value: 1.1E-65 score: 234.2
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 163..281 e-value: 5.6E-22 score: 78.5
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 75..282 e-value: 1.3E-81 score: 275.3
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..286
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..286
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..282 score: 12.739725

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016333.1	HG10016333.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen