HG10015217 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10015217
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr02: 24901415 .. 24904120 (-)
RNA-Seq ExpressionHG10015217
SyntenyHG10015217
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACAAAGAAGCAAGAATTTAACTCTTTCACTGTTTCTGTTTTTGGTGGTCTCTTTGCTGGTGTCTTCTTCTATAGTTAGAGTGGAGGCAAGGAAGCAATATCACAGCAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGCTGGCAATGGCAATGGTTCAGGCTCTGCACCAGCTCCACTTCCTGATTACAATCAAACTCAGTCCACCGTTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAATTTCTGATGATTCCAAGGTAAAAAATTGTCCAAAACAACCTCAAAAAACAGATACAGAGCTAAAAATGGTGACTGATGTACTGTTTGAACTTGTAAAAACCATTCTATGCAGGCCCTTCTAGCAGCATGGAAAGCTGCATGCGAGATTCCAGGAGCTACATTGGAAATACCATCACAATACAAGTTTCTTATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAGCTCGTTGTTCAGGTTTGTACAATTTTCATCTCCTTCAGGATTTTGGTATATTTGAGAACGTAACAAAAAAGACAAAAAAGTTTTCTTTGACTTGGCATGCAACAGATTGGTTTTTTTTTTAAAGAAAAAAAGAAATTAGTGCTCTGCTTAGAGTTGAAAGGATAAGATAATTAATGTGAATTGCAATTCCAAAGCAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTAAGTACGTGGCCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGCCAAGGCTTTAATTGGTGGACTCTCTCCACATTCTACACTAACAAAGTTAGTTCTTGTTTTCCCCTCTTCAATAACCAATTCTGTAATCTTTATTAAATTAGGCTAATTACATAGAGAGATCATGCTTTTAACTAAATTATTTATTGTTATTTTTGTTTCTGCAGAAGAAATTGAAGCACATTCCAGATATGAAGCCTACTGTTAAGTTTTCTTACTCTTTCTCTCTCATTTTGCTTATAATTACATTTTTTTTTCTGAATTTAATTGGGATTTTTTTTAATATTTTGCTTATAAATGACAGGCTTTAAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAGATTCAACACTCCAACATTGGCTGTGGTATGAACCGACCCAATTCACAGACCTTCTTTAATAATATTTTATTGTCAATTCAAGAGTTAACTTTTTTTTTCCTGTATCTTTTTTGATCTGTTTTATGGTTTTGAGGACACAAGAAAGGGGTGCTTTGAAAAAGAAAATTTTGACTCAAATTTCAAAAATACTTTTTAAGATATAAAAATAATATAAAATGTTTAAAATAATAAGTAGAAAACTAAACGTTGGTTCAAATTAAAATTTGATCCGTAGTACTATGGTTATTACGATTATTACTATATATCGATGTATTTTGAAGCTTTGTATTTTGAGCATTAGTTTCTTTTCATTACATCAATGAAAAGTTTGTTTCCGTTTAAAAAAAAAAAAGCCCTCTAATCTAAACACTTGTGAAAAAAAATATTTCCAATAAAAACGTTAATATAACTTACAAATTTTGTTTTCAAAATTATTTAAATAACAAAGTTGTTTGTTTGCACTACATTAAAAAAGGATTCTATCAATTATTACCATATTTTGAGAAGTTTTGGTTAAACATTTTAAAGTCATTGGTTGATAAATCAACTCTGTTTCTTTAAAAAAAGGTTTTTAAATTAATTCAATTTTTTTGAACTATTTCTTATTTGTTAGTTCGGTATTCTAAAACCACTTATACTAAATACACTTTAAATTATTAATTTTTTCAAAAGACACTTCAAAGAAAATTCAATTTTAAAAAGTATTTTTTAAATTTTTTTTCCAAACACACTATTAATTCAAATTGTCACGTGTCTTGATAAATGTCAGTTCTAAGGAAAATGATCTTATAATTTTCTTGGAAAACAAATGTTTTACTCTAATTCACATATGTTCACACTTGTATATTCAATGGTGGAAAATTATGAGGTAAAATTGTAAAGATAACTCCATGGAGGTTAAAAAAGCAGACCTCCATTTAACAATATTTTGATTGCAACAGCACCTAAAGCTACACCACAAAGAAACTGGACTACACAATCGAACCAACTTATTTTAAGATTTGTTATTTTAACAGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGGTAAAAATATAATCCTCATTAATTTTACTTTTTCATAAAGCTAATTTCTGTAGAGAATATTATTTATGCCTTTGATTTTGCTCAACTTTTCTTAAACATTATTATATTCCGCACTAAAGATTATTTTCTTGCCTATAATTACAGCTTGGGCGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGTAAAAGAAAAAGGGTTAAAGTTAAACTACTAA

mRNA sequence

ATGAAACAAAGAAGCAAGAATTTAACTCTTTCACTGTTTCTGTTTTTGGTGGTCTCTTTGCTGGTGTCTTCTTCTATAGTTAGAGTGGAGGCAAGGAAGCAATATCACAGCAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGCTGGCAATGGCAATGGTTCAGGCTCTGCACCAGCTCCACTTCCTGATTACAATCAAACTCAGTCCACCGTTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAATTTCTGATGATTCCAAGGCCCTTCTAGCAGCATGGAAAGCTGCATGCGAGATTCCAGGAGCTACATTGGAAATACCATCACAATACAAGTTTCTTATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAGCTCGTTGTTCAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTAAGTACGTGGCCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGCCAAGGCTTTAATTGGTGGACTCTCTCCACATTCTACACTAACAAAAAGAAATTGAAGCACATTCCAGATATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAGATTCAACACTCCAACATTGGCTGTGATTATTTTCTTGCCTATAATTACAGCTTGGGCGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGTAAAAGAAAAAGGGTTAAAGTTAAACTACTAA

Coding sequence (CDS)

ATGAAACAAAGAAGCAAGAATTTAACTCTTTCACTGTTTCTGTTTTTGGTGGTCTCTTTGCTGGTGTCTTCTTCTATAGTTAGAGTGGAGGCAAGGAAGCAATATCACAGCAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGCTGGCAATGGCAATGGTTCAGGCTCTGCACCAGCTCCACTTCCTGATTACAATCAAACTCAGTCCACCGTTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAATTTCTGATGATTCCAAGGCCCTTCTAGCAGCATGGAAAGCTGCATGCGAGATTCCAGGAGCTACATTGGAAATACCATCACAATACAAGTTTCTTATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAGCTCGTTGTTCAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTAAGTACGTGGCCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGCCAAGGCTTTAATTGGTGGACTCTCTCCACATTCTACACTAACAAAAAGAAATTGAAGCACATTCCAGATATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAGATTCAACACTCCAACATTGGCTGTGATTATTTTCTTGCCTATAATTACAGCTTGGGCGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGTAAAAGAAAAAGGGTTAAAGTTAAACTACTAA

Protein sequence

MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCDYFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQVKEKGLKLNY
Homology
BLAST of HG10015217 vs. NCBI nr
Match: KAG7032297.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566.2 bits (1458), Expect = 1.8e-157
Identity = 277/314 (88.22%), Postives = 292/314 (92.99%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           MKQRSKNLTLSL LFLVVSLLVSSS+VRVEARKQYHSKK KHQKQKG  NGNGSG AP P
Sbjct: 5   MKQRSKNLTLSLVLFLVVSLLVSSSVVRVEARKQYHSKKNKHQKQKGVDNGNGSGPAPPP 64

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
            PDYNQTQST+FNI+SFGAKGNG+ DDSKALLAAW+AACE  GATLEIPSQYKFLIKPIT
Sbjct: 65  FPDYNQTQSTIFNIMSFGAKGNGVFDDSKALLAAWEAACENTGATLEIPSQYKFLIKPIT 124

Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
           L GPCMPQLV+QIDGAVLAP +VS+WPKSSLFQWLNFKWLHNFTIQGSGTVDG GFNWW 
Sbjct: 125 LKGPCMPQLVLQIDGAVLAPTRVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGMGFNWWA 184

Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
            S F  NKKK+K+ PDMKP ALRFYSS+NVTVRDITIINSPQCHLKFDNSGTV+VDNITI
Sbjct: 185 RSRFDNNKKKMKNTPDMKPAALRFYSSFNVTVRDITIINSPQCHLKFDNSGTVQVDNITI 244

Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGCDYFLAYNYSLGGLGKDKSAACVSNIVVQNIS 300
           SSPENSPNTDGIHLQNTQDVEIQHSNI CD FLAYNYSLGGLGKD++AACVSNIVV+NIS
Sbjct: 245 SSPENSPNTDGIHLQNTQDVEIQHSNISCDDFLAYNYSLGGLGKDRTAACVSNIVVENIS 304

Query: 301 IQNTLSGVRIKTWQ 315
           IQNTLSGVRIKTWQ
Sbjct: 305 IQNTLSGVRIKTWQ 318

BLAST of HG10015217 vs. NCBI nr
Match: XP_008446184.2 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo])

HSP 1 Score: 539.3 bits (1388), Expect = 2.3e-149
Identity = 269/317 (84.86%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 17  VVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILS 76
           +VSLLVSSS+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILS
Sbjct: 1   MVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILS 60

Query: 77  FGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGA 136
           FGAKGNG+SDDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGA
Sbjct: 61  FGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGA 120

Query: 137 VLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPD 196
           VLAPPKVS+WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPD
Sbjct: 121 VLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPD 180

Query: 197 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQN 256
           MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQN
Sbjct: 181 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQN 240

Query: 257 TQDVEIQHSNIGC-------------------DYFLAYNYSLGGLGKDKSAACVSNIVVQ 315
           T+DVEIQHSNIGC                   +    +  SLGGLGKDKSAACVSNIVVQ
Sbjct: 241 TRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 300

BLAST of HG10015217 vs. NCBI nr
Match: TYK15622.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-145
Identity = 261/308 (84.74%), Postives = 275/308 (89.29%), Query Frame = 0

Query: 26  IVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILSFGAKGNGIS 85
           +VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILSFGAKGNG+S
Sbjct: 3   LVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVS 62

Query: 86  DDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVST 145
           DDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGAVLAPPKVS+
Sbjct: 63  DDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSS 122

Query: 146 WPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPDMKPTALRFY 205
           WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPDMKPTALRFY
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFY 182

Query: 206 SSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHS 265
           SSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQNT+DVEIQHS
Sbjct: 183 SSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHS 242

Query: 266 NIGC-------------------DYFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLS 315
           NIGC                   +    +  SLGGLGKDKSAACVSNIVVQNISIQNTLS
Sbjct: 243 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 302

BLAST of HG10015217 vs. NCBI nr
Match: XP_022944897.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita moschata])

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-145
Identity = 267/344 (77.62%), Postives = 285/344 (82.85%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           M + SK LTL L     VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS  APAP
Sbjct: 1   MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
           LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
           LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180

Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
           +ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITI
Sbjct: 181 VSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITI 240

Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGC------------------------------D 300
           SSPE+SPNTDGIHLQNTQDVEIQHSNIGC                               
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTRG 300

Query: 301 YFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
            FLAYNYSLGGLGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQ
Sbjct: 301 LFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQ 339

BLAST of HG10015217 vs. NCBI nr
Match: XP_022944896.1 (polygalacturonase At1g48100 isoform X1 [Cucurbita moschata])

HSP 1 Score: 521.9 bits (1343), Expect = 3.8e-144
Identity = 267/345 (77.39%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           M + SK LTL L     VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS  APAP
Sbjct: 1   MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
           LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVV-QIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWW 180
           LNGPCMPQLV+ QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180

Query: 181 TLSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           T+ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 TVSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGC------------------------------ 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGC                              
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTR 300

Query: 301 DYFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
             FLAYNYSLGGLGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQ
Sbjct: 301 GLFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQ 340

BLAST of HG10015217 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.7e-46
Identity = 123/339 (36.28%), Postives = 168/339 (49.56%), Query Frame = 0

Query: 12  LFLFLVVSLLVSSSIVRVEARKQYHSKKGKH-----------QKQKGAGNGNGSGSAPAP 71
           + +  ++S ++      V AR +YH  KG+H              + A   +   S P P
Sbjct: 7   ILVIYIISTVLFGHFTNV-ARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYP 66

Query: 72  LPD---YNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 131
            PD    +     VF++ SFGA G+G  DD+ A   AWKAAC +    +  P    F I 
Sbjct: 67  SPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKIT 126

Query: 132 PITLNGPCMPQLVVQIDGAVLAPPKVSTWP-KSSLFQWLNFKWLHNFTIQGSGTVDGQGF 191
               +GPC P LV Q+DG ++ P     WP K +  QWL F  L  FT  G GTV+G G 
Sbjct: 127 STIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQ 186

Query: 192 NWWTL--STFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVK 251
            WW L               P   PT +RF+ S N+ V+ + I NSPQ H+KFD    V 
Sbjct: 187 KWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVL 246

Query: 252 VDNITISSPENSPNTDGIHLQNTQDVEIQHSNI-----------GCDYF--------LAY 311
           ++ I ISSP+ SPNTDGIHL NT+ V I +S +           GC            ++
Sbjct: 247 INEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSH 306

Query: 312 NYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
             S+G LG   S ACVSNI V+N  I+++ +G+R+KTWQ
Sbjct: 307 GISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQ 344

BLAST of HG10015217 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-41
Identity = 92/265 (34.72%), Postives = 144/265 (54.34%), Query Frame = 0

Query: 69  STVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQ 128
           +TVFN+  +GA G+G  D ++A    W AAC+   A L +P+  KF +  +   GPC P 
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115

Query: 129 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNK 188
           L  ++DG ++A P  + W  S +  WL F  L +F + G+G +DGQG  WW       N 
Sbjct: 116 LSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNG 175

Query: 189 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 248
           + + +  + +PTA++   S +VTV+++T++NSP+ HL F     VK+  + I +P +SPN
Sbjct: 176 RTVCNDRN-RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPN 235

Query: 249 TDGIHLQNTQDVEIQHSNIGC-DYFLA------------------YNYSLGGLGKDKSAA 308
           TDGI +  ++   I+   IG  D  +A                  +  S+G LG+D S A
Sbjct: 236 TDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRA 295

Query: 309 CVSNIVVQNISIQNTLSGVRIKTWQ 315
            VS++ V      +T +G+RIKTWQ
Sbjct: 296 EVSHVHVNRAKFIDTQNGLRIKTWQ 317

BLAST of HG10015217 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.9e-41
Identity = 90/263 (34.22%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 71  VFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLV 130
           +FN+  +GA G+G  D ++A   AW+AAC+ P A L +P   KF++  +  NGPC P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 131 VQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKK 190
            ++DG + A    ++W  + +  WL F  L  FT+ G G +DGQG  WW     + N ++
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177

Query: 191 LKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTD 250
           + +  D +PTA++F  S  + ++ + ++NSP+ HL F N   VK+  I+I++P +SPNTD
Sbjct: 178 ICNDRD-RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTD 237

Query: 251 GIHLQNTQDVEIQHSNIGC-DYFLA------------------YNYSLGGLGKDKSAACV 310
           GI +  +++  +Q + IG  D  +A                  +  S+G LG++ S A V
Sbjct: 238 GIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEV 297

Query: 311 SNIVVQNISIQNTLSGVRIKTWQ 315
           S + V      +T +G+RIKTWQ
Sbjct: 298 SYVHVNGAKFIDTQNGLRIKTWQ 317

BLAST of HG10015217 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-40
Identity = 93/266 (34.96%), Postives = 139/266 (52.26%), Query Frame = 0

Query: 71  VFNILSFGAKGNGISDDSKALLAAWKAACEIPGAT-LEIPSQYKFLIKPITLNGPCMPQL 130
           + ++ +FGAKGNG++DD+KA   AWK AC     T + +P  Y  L++PI L+GPC  +L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 131 VVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKK 190
            +QI G ++AP     W   +  +WL F  L   T++G GTV+G G  WW  S  + +  
Sbjct: 112 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 171

Query: 191 KLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNT 250
             +      PTAL F+   N+ V ++ +I+S Q H+   +   V +  + + +P  SPNT
Sbjct: 172 PCRG----APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNT 231

Query: 251 DGIHLQNTQDV---------------------EIQHSNIGCDYFLAYNYSLGGLGKDKSA 310
           DGIH+  ++ +                     +I  SNI C     +  S+G LGK KS 
Sbjct: 232 DGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICG--PGHGISIGSLGKSKSW 291

Query: 311 ACVSNIVVQNISIQNTLSGVRIKTWQ 315
             V +I V    I +T +GVRIKTWQ
Sbjct: 292 EEVRDITVDTAIISDTANGVRIKTWQ 311

BLAST of HG10015217 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-40
Identity = 100/266 (37.59%), Postives = 142/266 (53.38%), Query Frame = 0

Query: 69  STVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQ 128
           S   N+  FGAKG+G  DD+KA   AWKAAC    + + +  +  +L++PI+ +GPC   
Sbjct: 87  SKTVNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 146

Query: 129 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNK 188
           L +QI G + A    S + K     WL F  + N  ++G GT++G G  WW  S      
Sbjct: 147 LTMQIYGTIEASDDRSDYRKDGR-HWLVFDSVQNLRVEGGGTINGNGKIWWQNSC----- 206

Query: 189 KKLKHIP-DMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSP 248
           K  K +P    PTAL FY S +V V+++ I N+ Q H+ FDN   V+  N+ +++PENSP
Sbjct: 207 KTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP 266

Query: 249 NTDGIHLQNTQDVEIQHSNIG----CDYFL---------------AYNYSLGGLGKDKSA 308
           NTDGIH+  TQ++ I    IG    C   +                +  S+G LG   S 
Sbjct: 267 NTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSE 326

Query: 309 ACVSNIVVQNISIQNTLSGVRIKTWQ 315
           A VS++VV    +  T +GVRIKTWQ
Sbjct: 327 AHVSDVVVNGAKLCGTTNGVRIKTWQ 345

BLAST of HG10015217 vs. ExPASy TrEMBL
Match: A0A1S3BEF8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.1e-149
Identity = 269/317 (84.86%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 17  VVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILS 76
           +VSLLVSSS+VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILS
Sbjct: 1   MVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILS 60

Query: 77  FGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGA 136
           FGAKGNG+SDDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGA
Sbjct: 61  FGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGA 120

Query: 137 VLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPD 196
           VLAPPKVS+WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPD
Sbjct: 121 VLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPD 180

Query: 197 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQN 256
           MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQN
Sbjct: 181 MKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQN 240

Query: 257 TQDVEIQHSNIGC-------------------DYFLAYNYSLGGLGKDKSAACVSNIVVQ 315
           T+DVEIQHSNIGC                   +    +  SLGGLGKDKSAACVSNIVVQ
Sbjct: 241 TRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQ 300

BLAST of HG10015217 vs. ExPASy TrEMBL
Match: A0A5D3CVS9 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001190 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.8e-146
Identity = 261/308 (84.74%), Postives = 275/308 (89.29%), Query Frame = 0

Query: 26  IVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQSTVFNILSFGAKGNGIS 85
           +VRVEARKQYH KK KHQKQKG GNG+GSG APAPLP YNQ+QST+FNILSFGAKGNG+S
Sbjct: 3   LVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVS 62

Query: 86  DDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVST 145
           DDSKALLAAWKAACEIPGATLEIPSQ+KFLIKPITLNGPCM QLV+QIDGAVLAPPKVS+
Sbjct: 63  DDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSS 122

Query: 146 WPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNKKKLKHIPDMKPTALRFY 205
           WPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFY NKKKLKHIPDMKPTALRFY
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFY 182

Query: 206 SSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHS 265
           SSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQNT+DVEIQHS
Sbjct: 183 SSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHS 242

Query: 266 NIGC-------------------DYFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLS 315
           NIGC                   +    +  SLGGLGKDKSAACVSNIVVQNISIQNTLS
Sbjct: 243 NIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLS 302

BLAST of HG10015217 vs. ExPASy TrEMBL
Match: A0A6J1FZF8 (polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 7.6e-146
Identity = 267/344 (77.62%), Postives = 285/344 (82.85%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           M + SK LTL L     VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS  APAP
Sbjct: 1   MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
           LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
           LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180

Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
           +ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITI
Sbjct: 181 VSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITI 240

Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGC------------------------------D 300
           SSPE+SPNTDGIHLQNTQDVEIQHSNIGC                               
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTRG 300

Query: 301 YFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
            FLAYNYSLGGLGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQ
Sbjct: 301 LFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQ 339

BLAST of HG10015217 vs. ExPASy TrEMBL
Match: A0A6J1FZB4 (polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.9e-144
Identity = 267/345 (77.39%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           M + SK LTL L     VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS  APAP
Sbjct: 1   MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
           LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAWKAAC+IPGATL+IPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVV-QIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWW 180
           LNGPCMPQLV+ QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI G GTVDGQGFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180

Query: 181 TLSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           T+ST Y NKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 TVSTLYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGC------------------------------ 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGC                              
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTR 300

Query: 301 DYFLAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
             FLAYNYSLGGLGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQ
Sbjct: 301 GLFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQ 340

BLAST of HG10015217 vs. ExPASy TrEMBL
Match: A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.0e-142
Identity = 260/333 (78.08%), Postives = 280/333 (84.08%), Query Frame = 0

Query: 1   MKQRSKNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAP 60
           M + SK LTL L     VSLLVSSS+V +EARK +H+KK KHQKQ+G G G+GS  APAP
Sbjct: 1   MNKGSKILTLFL-----VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  LPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPIT 120
           LPDYNQTQST+FNILSFGAKGNG+ DDSKAL+AAW AAC+IPGATL+IP  YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPIT 120

Query: 121 LNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWT 180
           LNGPCMPQLV+QIDG VLAPPKVS+W KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180

Query: 181 LSTFYTNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITI 240
           +ST Y NKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNITI
Sbjct: 181 VSTLYNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITI 240

Query: 241 SSPENSPNTDGIHLQNTQDVEIQHSNIGC-------------------DYFLAYNYSLGG 300
           SSPE+SPNTDGIHLQNTQDVEIQHSNIGC                   +    +  SLGG
Sbjct: 241 SSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 300

Query: 301 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
           LGKDKS ACVSNIVV+NISIQNTLSGVRIKTWQ
Sbjct: 301 LGKDKSVACVSNIVVENISIQNTLSGVRIKTWQ 328

BLAST of HG10015217 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.6e-100
Identity = 187/328 (57.01%), Postives = 238/328 (72.56%), Query Frame = 0

Query: 6   KNLTLSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYN 65
           K+L +   L  V+ +  + S V VE RK +H KK K + ++ + N      APAP P  N
Sbjct: 5   KSLPILAMLLGVIIVAAAISTVSVEGRK-HHVKKIKPKHRRHSKNTPTGSPAPAPYPSTN 64

Query: 66  QTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNGPC 125
           +    VFNI S+GAKG+G+SDDSKAL+ AWKAAC++ G  +EIP+  +FL+K +TL GPC
Sbjct: 65  E---GVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPC 124

Query: 126 MPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFY 185
             + VVQI+G ++AP K+ +WP SSLFQWLNFKW+ + TIQGSGT++G+G+NWW L T+ 
Sbjct: 125 KEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTYQ 184

Query: 186 TNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPEN 245
           T  +  K+IP MKPTALRFYSS NVTVRDI+I+NSP CHLKFD+S  VKV+NITISSPEN
Sbjct: 185 TQTRN-KYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPEN 244

Query: 246 SPNTDGIHLQNTQDVEIQHSNIGC-------------------DYFLAYNYSLGGLGKDK 305
           SPNTDGIHLQNT++VEIQHSNI C                   +    +  S+GGLGKDK
Sbjct: 245 SPNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDK 304

Query: 306 SAACVSNIVVQNISIQNTLSGVRIKTWQ 315
           S ACVS+I+V++ISIQNTL+GVRIKTWQ
Sbjct: 305 SVACVSDIIVEDISIQNTLAGVRIKTWQ 327

BLAST of HG10015217 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.0e-62
Identity = 130/302 (43.05%), Postives = 176/302 (58.28%), Query Frame = 0

Query: 58  PAPLPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 117
           P PLP      S  FN+L FGAKG+G+SDD++A  AAW +AC++  +T+ IP  Y FL+ 
Sbjct: 109 PTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVG 168

Query: 118 PITLNGP-CMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGF 177
           PI+ +GP C   +V Q++G ++AP    +W    L  W+ F  L   TIQG+G +DG+G 
Sbjct: 169 PISFSGPYCQANIVFQLEGMIVAPTDTESW-GGGLMWWIEFTKLSGITIQGNGVIDGRGT 228

Query: 178 NWWT---LSTFYTN---------------------KKKLK-HIPDMKPTALRFYSSYNVT 237
            WW    LS +  +                     + +L   +P +KPTALRFY S +VT
Sbjct: 229 VWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVT 288

Query: 238 VRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNI---- 297
           V  ITI NSPQCHLKFDN   V V ++ +SSP +SPNTDGIHLQNT+DV I  + +    
Sbjct: 289 VTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGD 348

Query: 298 -------GCDYFLAYN--------YSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKT 315
                  GC     +N         S+G LGKD + ACVSNI V+++ + NT++GVRIKT
Sbjct: 349 DCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKT 408

BLAST of HG10015217 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.1e-62
Identity = 129/299 (43.14%), Postives = 174/299 (58.19%), Query Frame = 0

Query: 58  PAPLPDYNQTQSTVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIK 117
           P PLP   Q  S VFN++ FGAKG+G  DD+KA  AAW AAC+I  + + +P +Y +L+ 
Sbjct: 107 PPPLPP--QKGSQVFNVMDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVG 166

Query: 118 PITLNGP-CMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGF 177
           PI+ +GP C   +V Q+DG ++AP    TW K  L  W++F  L    +QG G +DG+G 
Sbjct: 167 PISFSGPYCQANIVFQLDGTIIAPTDSKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGRGS 226

Query: 178 NWWTL-STFYTNKKKL---------------------KHIPDMKPTALRFYSSYNVTVRD 237
            WW   S F  +  KL                     + +P +KPTALRF  S+ V V  
Sbjct: 227 GWWQQDSPFIDSDTKLIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTG 286

Query: 238 ITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNI------- 297
           ITI NSPQCHLKFD+   V V +I +SSP +SPNTDGIHLQNT+DV I  + +       
Sbjct: 287 ITIQNSPQCHLKFDDCVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCI 346

Query: 298 ----GCDYFLAYN--------YSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
               GC     +N         S+G LGK+ + ACVSNI V+++++ NT++GVRIKTWQ
Sbjct: 347 SIQTGCSNVFVHNVNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQ 402

BLAST of HG10015217 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-58
Identity = 132/325 (40.62%), Postives = 178/325 (54.77%), Query Frame = 0

Query: 10  LSLFLFLVVSLLVSSSIVRVEARKQYHSKKGKHQKQKGAGNGNGSGSAPAPLPDYNQTQS 69
           LSL   L++SL  SS     +AR   + K  +    +G  +G                  
Sbjct: 9   LSLNFLLLISLF-SSRFGTCDARYSVYWKGNRRSIAEGGSSG------------------ 68

Query: 70  TVFNILSFGAKGNGISDDSKALLAAWKAACEIPGATLEIPSQYKFLIKPITLNG-PCMPQ 129
              N+L  GAKG+G SDD+KA   AW+ AC++  +TL +PS   FL+ P++  G  C  +
Sbjct: 69  -TINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKEK 128

Query: 130 LVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYTNK 189
           +V Q++G ++AP   S W  S L QW+ FK L   TI+G G +DG+G  WW       N 
Sbjct: 129 IVFQLEGKIIAPTSASAW-GSGLLQWIEFKALQGITIKGKGIIDGRGSVWW-------ND 188

Query: 190 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 249
                +P  KPTALRFY S  VTV  ITI NSPQ HLKFDN  +++V + T SSP +SPN
Sbjct: 189 MMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPN 248

Query: 250 TDGIHLQNTQDVEIQHSNIGC-------------------DYFLAYNYSLGGLGKDKSAA 309
           TDGIHLQN+QD  I  S + C                   D    +  S+GGLGKD + A
Sbjct: 249 TDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKA 305

Query: 310 CVSNIVVQNISIQNTLSGVRIKTWQ 315
           CVSNI V+++++  T +GVRIK+WQ
Sbjct: 309 CVSNITVRDVTMHETTNGVRIKSWQ 305

BLAST of HG10015217 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 116/287 (40.42%), Postives = 163/287 (56.79%), Query Frame = 0

Query: 57  APAPLPDYNQTQS---TVFNILSFGAKGNGISDDSKALLAAWKAACEIPG---ATLEIPS 116
           +PAP P YN   +   TVF++ SFGA G+  +DD+ A   AW AAC   G   A L +P 
Sbjct: 30  SPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVPY 89

Query: 117 QYKFLIKPITLNGPCMPQLVVQIDGAVLAPPKVSTWPKSSLFQWLNFKWLHNFTIQGSGT 176
            + FL+KP T NGPC   LV+QIDG +++P    +WP +   QW+ F  ++  +IQGSG 
Sbjct: 90  TFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSGV 149

Query: 177 VDGQGFNWWTLSTFYTNKKKLKHI----PDMKPTALRFYSSYNVTVRDITIINSPQCHLK 236
           ++G+G  WW L       K L       P   P A+R + S  V ++ I  +NS Q H++
Sbjct: 150 INGRGQKWWNLPC--KPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVR 209

Query: 237 FDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHS---------NIGCDYF----- 296
           FDN   V VD++ I +P +SPNTDGIH++NT +V+I++S         +IG   F     
Sbjct: 210 FDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIK 269

Query: 297 -----LAYNYSLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQ 315
                 ++  S+G LG   S A VSNI V N +I N+ +GVRIKTWQ
Sbjct: 270 NVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQ 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032297.11.8e-15788.22Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008446184.22.3e-14984.86PREDICTED: polygalacturonase At1g48100 [Cucumis melo][more]
TYK15622.11.2e-14584.74polygalacturonase [Cucumis melo var. makuwa][more]
XP_022944897.11.6e-14577.62polygalacturonase At1g48100 isoform X2 [Cucurbita moschata][more]
XP_022944896.13.8e-14477.39polygalacturonase At1g48100 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q949Z11.7e-4636.28Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q7M1E72.2e-4134.72Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P432124.9e-4134.22Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q94AJ51.1e-4034.96Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353361.8e-4037.59Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BEF81.1e-14984.86polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1[more]
A0A5D3CVS95.8e-14684.74Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
A0A6J1FZF87.6e-14677.62polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZB41.9e-14477.39polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1HSE81.0e-14278.08polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G26610.13.6e-10057.01Pectin lyase-like superfamily protein [more]
AT1G60590.13.0e-6243.05Pectin lyase-like superfamily protein [more]
AT1G10640.15.1e-6243.14Pectin lyase-like superfamily protein [more]
AT5G14650.12.0e-5840.62Pectin lyase-like superfamily protein [more]
AT1G56710.14.8e-5240.42Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 291..312
e-value: 42.0
score: 13.0
coord: 207..256
e-value: 46.0
score: 12.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 278..314
e-value: 2.3E-5
score: 23.6
coord: 104..268
e-value: 5.9E-29
score: 101.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 59..320
e-value: 4.1E-64
score: 219.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..60
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 25..270
coord: 277..316
NoneNo IPR availablePANTHERPTHR31375:SF92PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 277..316
coord: 25..270
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 64..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10015217.1HG10015217.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity