HG10014031 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10014031
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCRAL-TRIO domain-containing protein
LocationChr02: 6970685 .. 6972004 (-)
RNA-Seq ExpressionHG10014031
SyntenyHG10014031
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGGTCGGTGGTCTACTTCTCATCACGAGGAAGAGAACAATCCAGAACAGATCACATCGAAGGTATGAATCTGATGAGTTGATGATCAAAGAAAATTATGTATGTAGGCTTCTCTGTTAGTTCCCAGATCCATTTACATAGAAACTTTGAACTTGCATGAGCCTGATTGGATCTAAACCATCTTAAAGATTTTTCGGGATCGTGTGTTTTATACCAGTTTTTAAGATGATTGAGAGTCATAGATGATAAGATTTTACGCTTTTGCAGGTAAATGAGCTAAAGGCTAAGTTAGGATCCTTAGTTGGACGCAATGCATTATATTGCAGTGATGCATGCTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACATTCAAATGGAGAGCTACATATAAGCCTGAGGAAATTCGTTGGGTAAGCATGCAGTAGTTTCATAAAACTAGTAGTGATGGAGTTTTATGTTGGCTTTGAACTAATCATTTTTTTTTTGTTCATTTTAGCCTGAAATAGCATCTGAAAGTGAGACTGGTAAACTTTATCGAGCAAGTTTTCACGACCGAGAAGGAAGAACTATTCTTATAATGAAGCCAGGAAAGCAGGTATTTTATTAGTGTAAATTCTGCATCCTGGTTCTTTGCAATTTTTGCATTAATTGGAGTTAACAGCCAATACAATACTTTTTTCTTACTTTTTTACAGAACACGACATCAATAGAAAATCAGATCCGGCATTTGGTATATCTTATGGAGAATGCTCTTCTCAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTAATTGATTTTAATGGTTGGTCTCTTAACACCAGCGTGCCAATCAAATCTGCACGGGAGACCGTCAGCATCTTGCAAAACCACTATCCCGAGAGACTAGCGCTTGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCGTTTTGGAAGGTATGGTATATGCTTTCCAAAATCATGCAATATCTCTTCCATCCATATAATTTTGTTTATTACTTGTAGGTAGTCAAGTACCTTTTAGATCCCAAAACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAAAGTGTTGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTCCATGAGGAGTACTCTAGATTAATGATTCAAGATGATATTAAATGTGTTGCCTTTTGGGAACATGGTGAAAAGCAACACCATCTTGTCAATGGCTACTCCAGAGCAGTGGTGGCTCCAGAGTGA

mRNA sequence

ATGTTTGGTCGGTGGTCTACTTCTCATCACGAGGAAGAGAACAATCCAGAACAGATCACATCGAAGGTAAATGAGCTAAAGGCTAAGTTAGGATCCTTAGTTGGACGCAATGCATTATATTGCAGTGATGCATGCTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACATTCAAATGGAGAGCTACATATAAGCCTGAGGAAATTCGTTGGCCTGAAATAGCATCTGAAAGTGAGACTGGTAAACTTTATCGAGCAAGTTTTCACGACCGAGAAGGAAGAACTATTCTTATAATGAAGCCAGGAAAGCAGAACACGACATCAATAGAAAATCAGATCCGGCATTTGGTATATCTTATGGAGAATGCTCTTCTCAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTAATTGATTTTAATGGTTGGTCTCTTAACACCAGCGTGCCAATCAAATCTGCACGGGAGACCGTCAGCATCTTGCAAAACCACTATCCCGAGAGACTAGCGCTTGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCGTTTTGGAAGGTAGTCAAGTACCTTTTAGATCCCAAAACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAAAGTGTTGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTCCATGAGGAGTACTCTAGATTAATGATTCAAGATGATATTAAATGTGTTGCCTTTTGGGAACATGGTGAAAAGCAACACCATCTTGTCAATGGCTACTCCAGAGCAGTGGTGGCTCCAGAGTGA

Coding sequence (CDS)

ATGTTTGGTCGGTGGTCTACTTCTCATCACGAGGAAGAGAACAATCCAGAACAGATCACATCGAAGGTAAATGAGCTAAAGGCTAAGTTAGGATCCTTAGTTGGACGCAATGCATTATATTGCAGTGATGCATGCTTGAAAAGATATCTAGTGGCCAGGAATTGGAATGTAGACAAAGCAAAGAAAATGCTGGAAGAGACATTCAAATGGAGAGCTACATATAAGCCTGAGGAAATTCGTTGGCCTGAAATAGCATCTGAAAGTGAGACTGGTAAACTTTATCGAGCAAGTTTTCACGACCGAGAAGGAAGAACTATTCTTATAATGAAGCCAGGAAAGCAGAACACGACATCAATAGAAAATCAGATCCGGCATTTGGTATATCTTATGGAGAATGCTCTTCTCAACCTTCCTGAAGGTCAGGAGCAGATGTCATGGCTAATTGATTTTAATGGTTGGTCTCTTAACACCAGCGTGCCAATCAAATCTGCACGGGAGACCGTCAGCATCTTGCAAAACCACTATCCCGAGAGACTAGCGCTTGCGTTTCTATATAATCCCCCAAGGATATTTGAAGCGTTTTGGAAGGTAGTCAAGTACCTTTTAGATCCCAAAACATTTCAGAAAGTGAGATTTGTTTATCCTAAGAAGCAAGAAAGTGTTGAACTCATGAAGTCATATTTTGATGAGGAGAATCTTCCATCAGAGTTTGGAGGAAAAGCCCAACTGGAATATGTCCATGAGGAGTACTCTAGATTAATGATTCAAGATGATATTAAATGTGTTGCCTTTTGGGAACATGGTGAAAAGCAACACCATCTTGTCAATGGCTACTCCAGAGCAGTGGTGGCTCCAGAGTGA

Protein sequence

MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE
Homology
BLAST of HG10014031 vs. NCBI nr
Match: XP_038906163.1 (CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida] >XP_038906164.1 CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida])

HSP 1 Score: 572.4 bits (1474), Expect = 2.2e-159
Identity = 271/286 (94.76%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNA+YCSDACLKRYL ARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNAIYCSDACLKRYLAARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           +KMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  RKMLEETFKWRSMYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRF+YPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFIYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEEYSR+MIQDDIKC AFWEHGEKQHH+VNGYS AVVAPE
Sbjct: 241 AQLEYVHEEYSRIMIQDDIKCAAFWEHGEKQHHIVNGYSSAVVAPE 286

BLAST of HG10014031 vs. NCBI nr
Match: XP_031738483.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 [Cucumis sativus])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 268/286 (93.71%), Postives = 277/286 (96.85%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEE NPEQITSKVNELKAKLG +VGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 50  MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 109

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 110 KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 169

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 170 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 229

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 230 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 289

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE GEKQHH+VNGYS AVVAPE
Sbjct: 290 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 335

BLAST of HG10014031 vs. NCBI nr
Match: XP_031738484.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X2 [Cucumis sativus])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 268/286 (93.71%), Postives = 277/286 (96.85%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEE NPEQITSKVNELKAKLG +VGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 28  MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 87

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 88  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 147

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 148 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 207

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 208 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 267

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE GEKQHH+VNGYS AVVAPE
Sbjct: 268 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 313

BLAST of HG10014031 vs. NCBI nr
Match: XP_004147786.1 (CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_011652412.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738485.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738486.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_031738487.1 CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >KAE8651327.1 hypothetical protein Csa_001508 [Cucumis sativus])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 268/286 (93.71%), Postives = 277/286 (96.85%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEE NPEQITSKVNELKAKLG +VGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEEKNPEQITSKVNELKAKLGPIVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE GEKQHH+VNGYS AVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQGEKQHHIVNGYSSAVVAPE 286

BLAST of HG10014031 vs. NCBI nr
Match: XP_008466570.1 (PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466571.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466572.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_008466573.1 PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >KAA0041624.1 CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo var. makuwa] >TYK19633.1 CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo var. makuwa])

HSP 1 Score: 558.1 bits (1437), Expect = 4.3e-155
Identity = 269/286 (94.06%), Postives = 276/286 (96.50%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEENNPEQITSKVNELKAKLG LVGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE  EKQHH VNGYS AVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

BLAST of HG10014031 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.6e-30
Identity = 82/248 (33.06%), Postives = 127/248 (51.21%), Query Frame = 0

Query: 11  EEENNPEQITSKVNELK--------AKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKK 70
           E+E   E++     ELK        +K   L+    L+ +  C+ RYL A  W+V  AKK
Sbjct: 595 EQEEMYEEVLKYCLELKEIPVASNSSKKTDLIELERLWLTRECILRYLRATKWHVSNAKK 654

Query: 71  MLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQ 130
            + +T  WR  +    +   EI  E+ TGK     + D++GR  L + P +QNT +   Q
Sbjct: 655 RIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLGY-DKDGRPCLYLYPARQNTKTSPLQ 714

Query: 131 IRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALA 190
           IRHLV+ +E A+  +P G E ++ LI+F   S  ++  +   +E ++ILQ HY ERL  A
Sbjct: 715 IRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNPSVGQGKEVLNILQTHYCERLGRA 774

Query: 191 FLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQ 250
            + N P     F+K++   +DP T +K++F  P        +  Y  ++ L S FGG   
Sbjct: 775 LVINIPWAVWGFFKLISPFIDPITREKLKFNEP--------LDRYVPKDQLDSNFGGSLH 833

BLAST of HG10014031 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-25
Identity = 71/247 (28.74%), Postives = 128/247 (51.82%), Query Frame = 0

Query: 13  ENNPEQITSKVNELKAKLGSLVG-RNALYCSDACLKRYLVARNWNVDKAKKMLEETFKWR 72
           +N  E+      E+K+    L    +  +C+D C  RYL ARN+ V K++KML +T +WR
Sbjct: 42  KNLNEKQLEAFKEIKSNFSDLTDPTDIAFCTDMCFLRYLRARNYIVSKSEKMLRDTLEWR 101

Query: 73  ATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQI--RHLVYL 132
             ++P++I+      E  +      +  D++GR I+   P      ++ +++  ++LVY 
Sbjct: 102 KKFRPQDIQLGGDIREIGSAGCVYVNKRDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYW 161

Query: 133 MENAL--LNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALAFLYNP 192
           +E     ++ P+G EQ  +++D+  +  + ++ +K+  E +  L +H PER+  +   +P
Sbjct: 162 LEQGFSRMDEPKGIEQFCFIVDYKDFG-SGNMDMKTNLEAMHFLLDHCPERMGQSLFLDP 221

Query: 193 PRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQES----VELMKSYFDEENLPSEFGGKAQL 251
           P +F   WK++   L+  T  KVRF+  KK +      EL++ Y D ENL    GG    
Sbjct: 222 PALFWFAWKIISPFLNEVTLSKVRFINSKKVDGKRTFAELLE-YVDIENLEQNLGGNLDY 281

BLAST of HG10014031 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.3e-25
Identity = 85/263 (32.32%), Postives = 125/263 (47.53%), Query Frame = 0

Query: 16  PEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKKMLEETFKWR---- 75
           PE +    N   A    L      + S  C  RYL A  WN   A K L +T  WR    
Sbjct: 65  PENLNDLDNGTHANDRPLSDWEKFWLSRECFLRYLRANKWNTANAIKGLTKTLVWRREIG 124

Query: 76  ATYKPEE---IRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQIRHLVY 135
            T+  E+   +   ++A E+ETGK     F D   R +  MK G+QNT S   Q++ LVY
Sbjct: 125 LTHGKEDKDPLTADKVAVENETGKQVILGF-DNAKRPLYYMKNGRQNTESSFRQVQELVY 184

Query: 136 LMENALLNLPEGQEQMSWLIDFNGWS-----LNTSVPIKSARETVSILQNHYPERLALAF 195
           +ME A    P+G E+++ L+DF  +       + + PI  AR  ++++Q+HYPERLA   
Sbjct: 185 MMETATTVAPQGVEKITVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPERLAKCV 244

Query: 196 LYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQL 255
           L N P    AF K++   LDP T  K  F  P         +++ +   L + + G    
Sbjct: 245 LINIPWFAWAFLKMMYPFLDPATKAKAIFDEP--------FENHIEPSQLDALYNGLLDF 304

Query: 256 EYVHEEYSRLMIQ--DDIKCVAF 265
           +Y HE Y   M++  DD++   F
Sbjct: 305 KYKHEVYWPDMVKKVDDLRLKRF 318

BLAST of HG10014031 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 74/252 (29.37%), Postives = 120/252 (47.62%), Query Frame = 0

Query: 20  TSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKKMLEETFKWRATY----- 79
           TS+ N+ +  L  L      + +  C  RYL A  W +      +  T  WR  +     
Sbjct: 64  TSEKNKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLKDCIDRITMTLAWRREFGISHL 123

Query: 80  ---KPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQIRHLVYLME 139
                ++I    +A E+E+GK     + + + R IL +KPG+QNT +   Q++HLV+++E
Sbjct: 124 GEEHGDKITADLVAVENESGKQVILGY-ENDARPILYLKPGRQNTKTSHRQVQHLVFMLE 183

Query: 140 NALLNLPEGQEQMSWLIDFNGWSLNTSV-------PIKSARETVSILQNHYPERLALAFL 199
             +  +P GQ+ ++ LIDF  +     V       PI   +E + ILQ HYPERL  A L
Sbjct: 184 RVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHYPERLGKALL 243

Query: 200 YNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLE 257
            N P +   F K++   +DP T +K+ F  P           Y  +  L S +GG  + +
Sbjct: 244 TNIPWLAWTFLKLIHPFIDPLTREKLVFDEP--------FVKYVPKNELDSLYGGDLKFK 303

BLAST of HG10014031 vs. ExPASy Swiss-Prot
Match: P53989 (SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=SEC14 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.4e-17
Identity = 75/249 (30.12%), Postives = 121/249 (48.59%), Query Frame = 0

Query: 14  NNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKKMLEETFKWRAT 73
           N  E     + +L+++L +   +  L   D+ L R+L AR ++V  AK+M E   KWR  
Sbjct: 26  NTDEAQEGALKQLRSELEAAGFKERL--DDSTLLRFLRARKFDVALAKEMFENCEKWRKE 85

Query: 74  YKPEEIRWPEIASESE-TGKLYRASFH--DREGRTILIMKPGKQNTTSI------ENQIR 133
           Y    I       E     K Y   +H  D++GR +   + G  N T +      E  ++
Sbjct: 86  YGTNTIMQDFHYDEKPLVAKYYPQYYHKTDKDGRPVYFEELGAVNLTEMEKITTQERMLK 145

Query: 134 HLVYLMENAL-LNLPEGQEQMSWLI-------DFNGWSLNTSVPIKS-ARETVSILQNHY 193
           +LV+  E+ +   LP       +L+       D  G S++++  + S  RE   I QN+Y
Sbjct: 146 NLVWEYESVVNYRLPACSRAAGYLVETSCTVMDLKGISISSAYSVLSYVREASYISQNYY 205

Query: 194 PERLALAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPS 245
           PER+   +L N P  F   +++ K  LDP T  K+ F+     +S EL+K     ENLPS
Sbjct: 206 PERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKI-FILGSSYQS-ELLKQ-IPAENLPS 265

BLAST of HG10014031 vs. ExPASy TrEMBL
Match: A0A1S3CRQ6 (CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo OX=3656 GN=LOC103503951 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.1e-155
Identity = 269/286 (94.06%), Postives = 276/286 (96.50%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEENNPEQITSKVNELKAKLG LVGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE  EKQHH VNGYS AVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

BLAST of HG10014031 vs. ExPASy TrEMBL
Match: A0A5D3D813 (CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00200 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.1e-155
Identity = 269/286 (94.06%), Postives = 276/286 (96.50%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGR STSHHEEENNPEQITSKVNELKAKLG LVGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRRSTSHHEEENNPEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKPEEIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSIYKPEEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEE+S LMIQDDIKC AFWE  EKQHH VNGYS AVVAPE
Sbjct: 241 AQLEYVHEEFSTLMIQDDIKCAAFWEQDEKQHHTVNGYSSAVVAPE 286

BLAST of HG10014031 vs. ExPASy TrEMBL
Match: A0A6J1J0L2 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480825 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.0e-155
Identity = 265/286 (92.66%), Postives = 276/286 (96.50%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGRWSTSHHEE N+ EQITSKVNELKAKLG LVGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEGNSQEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKP+EIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSMYKPDEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKY LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYFLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEEYSRLMIQDD+KC AFWE GEKQHH+VNGY+ A VAPE
Sbjct: 241 AQLEYVHEEYSRLMIQDDVKCAAFWEPGEKQHHVVNGYTSAAVAPE 286

BLAST of HG10014031 vs. ExPASy TrEMBL
Match: A0A6J1FJV0 (CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446094 PE=4 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 7.9e-155
Identity = 264/286 (92.31%), Postives = 276/286 (96.50%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGRWSTSHHEE N+ EQITSKVNELKAKLG LVGRNA+YCSDACLKRYLVARNWNVDKA
Sbjct: 1   MFGRWSTSHHEEGNSQEQITSKVNELKAKLGPLVGRNAIYCSDACLKRYLVARNWNVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+ YKP+EIRWPEIA ESETGKLYRASFHDREGRT+LIMKPGKQNTTS+E
Sbjct: 61  KKMLEETFKWRSMYKPDEIRWPEIAFESETGKLYRASFHDREGRTVLIMKPGKQNTTSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFWKVVKY LDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWKVVKYFLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEEYSRLMIQDD+KC AFWE GEKQHH++NGY+ A VAPE
Sbjct: 241 AQLEYVHEEYSRLMIQDDVKCAAFWEPGEKQHHVINGYTSAAVAPE 286

BLAST of HG10014031 vs. ExPASy TrEMBL
Match: A0A6J1C7L8 (random slug protein 5 OS=Momordica charantia OX=3673 GN=LOC111008160 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-146
Identity = 249/286 (87.06%), Postives = 268/286 (93.71%), Query Frame = 0

Query: 1   MFGRWSTSHHEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKA 60
           MFGRWSTS HEE+N+ EQ  SK+NEL+AKLG LVGRN++YCSDACLKRYLVARNW+VDKA
Sbjct: 1   MFGRWSTSRHEEDNSEEQTASKINELRAKLGPLVGRNSIYCSDACLKRYLVARNWSVDKA 60

Query: 61  KKMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIE 120
           KKMLEETFKWR+  KPEEIRWP++A ESETGKLYRASF DREGRT+LIMKPGKQNT S+E
Sbjct: 61  KKMLEETFKWRSMCKPEEIRWPQVAHESETGKLYRASFQDREGRTVLIMKPGKQNTNSLE 120

Query: 121 NQIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLA 180
           NQI HLVYLMENA+LNLPEGQEQM WLIDFNGWSL+TSVPIKSARETV+ILQNHYPERLA
Sbjct: 121 NQIWHLVYLMENAILNLPEGQEQMLWLIDFNGWSLSTSVPIKSARETVNILQNHYPERLA 180

Query: 181 LAFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGK 240
           LAFLYNPPRIFEAFW+VVKY LDPKTFQKVRF+YPKKQESVELMKSYFDEENLPSEFGGK
Sbjct: 181 LAFLYNPPRIFEAFWRVVKYFLDPKTFQKVRFIYPKKQESVELMKSYFDEENLPSEFGGK 240

Query: 241 AQLEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           AQLEYVHEEYSRLMIQDDIKC AFWE GEKQH ++NGY  AVVAPE
Sbjct: 241 AQLEYVHEEYSRLMIQDDIKCAAFWESGEKQHQIINGYPSAVVAPE 286

BLAST of HG10014031 vs. TAIR 10
Match: AT1G75170.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 411.8 bits (1057), Expect = 4.6e-115
Identity = 194/288 (67.36%), Postives = 233/288 (80.90%), Query Frame = 0

Query: 4   RWSTSHHEEENNPEQI--TSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAK 63
           RW  S   E+   E     +K+ ELK  +G L GRN+LYCSDACLKRYL ARNWNV KAK
Sbjct: 3   RWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGKAK 62

Query: 64  KMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIEN 123
           KMLEET KWR+++KPEEIRW E++ E ETGK+Y+A FHDR GRT+LI++PG QNT S+EN
Sbjct: 63  KMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLEN 122

Query: 124 QIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLAL 183
           Q++HLVYL+ENA+LNLPE QEQMSWLIDF GWS++TSVPIKSARET++ILQNHYPERLA+
Sbjct: 123 QMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAV 182

Query: 184 AFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKA 243
           AFLYNPPR+FEAFWK+VKY +D KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA
Sbjct: 183 AFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKA 242

Query: 244 QLEYVHEEYSRLMIQDDIKCVAFWEHG---EKQHHLVNGYSRAVVAPE 287
            L+Y +EE+S+ M QDD+K   FW  G     Q H  NG+S A +APE
Sbjct: 243 LLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPE 290

BLAST of HG10014031 vs. TAIR 10
Match: AT1G75170.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 411.8 bits (1057), Expect = 4.6e-115
Identity = 194/288 (67.36%), Postives = 233/288 (80.90%), Query Frame = 0

Query: 4   RWSTSHHEEENNPEQI--TSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAK 63
           RW  S   E+   E     +K+ ELK  +G L GRN+LYCSDACLKRYL ARNWNV KAK
Sbjct: 3   RWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGKAK 62

Query: 64  KMLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIEN 123
           KMLEET KWR+++KPEEIRW E++ E ETGK+Y+A FHDR GRT+LI++PG QNT S+EN
Sbjct: 63  KMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLEN 122

Query: 124 QIRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLAL 183
           Q++HLVYL+ENA+LNLPE QEQMSWLIDF GWS++TSVPIKSARET++ILQNHYPERLA+
Sbjct: 123 QMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAV 182

Query: 184 AFLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKA 243
           AFLYNPPR+FEAFWK+VKY +D KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA
Sbjct: 183 AFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKA 242

Query: 244 QLEYVHEEYSRLMIQDDIKCVAFWEHG---EKQHHLVNGYSRAVVAPE 287
            L+Y +EE+S+ M QDD+K   FW  G     Q H  NG+S A +APE
Sbjct: 243 LLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPE 290

BLAST of HG10014031 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 354.4 bits (908), Expect = 8.7e-98
Identity = 161/277 (58.12%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 10  HEEENNPEQITSKVNELKAKLGSLVGRNALYCSDACLKRYLVARNWNVDKAKKMLEETFK 69
           H+ +N+  Q  +KV ELK+ +G L G + ++CSDA L+R+L ARNW+V+KAKKM++ET K
Sbjct: 8   HQLDNDDSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKAKKMIQETLK 67

Query: 70  WRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQIRHLVYL 129
           WR+TYKP+EIRW ++A E ETGK  RASFHDR+GR +LIM+P  QN+TS E  IRHLVYL
Sbjct: 68  WRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGNIRHLVYL 127

Query: 130 MENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALAFLYNPPR 189
           +ENA++NLP+GQ+QMSWLIDF GWS+  + P+K+ RE + ILQN+YPERL +AFLYNPPR
Sbjct: 128 LENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIAFLYNPPR 187

Query: 190 IFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEE 249
           +F+A ++  KY LDP+T +KV+FVYPK + S ELM ++FD ENLP EFGG+A LEY HE+
Sbjct: 188 LFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEATLEYDHED 247

Query: 250 YSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           +SR M +DD+K   +W    K +   NG+S + V PE
Sbjct: 248 FSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPE 284

BLAST of HG10014031 vs. TAIR 10
Match: AT1G75170.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 307.8 bits (787), Expect = 9.4e-84
Identity = 142/206 (68.93%), Postives = 173/206 (83.98%), Query Frame = 0

Query: 84  IASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQIRHLVYLMENALLNLPEGQEQ 143
           ++ E ETGK+Y+A FHDR GRT+LI++PG QNT S+ENQ++HLVYL+ENA+LNLPE QEQ
Sbjct: 2   VSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQMKHLVYLIENAILNLPEDQEQ 61

Query: 144 MSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALAFLYNPPRIFEAFWKVVKYLLD 203
           MSWLIDF GWS++TSVPIKSARET++ILQNHYPERLA+AFLYNPPR+FEAFWK+VKY +D
Sbjct: 62  MSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAFLYNPPRLFEAFWKIVKYFID 121

Query: 204 PKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQLEYVHEEYSRLMIQDDIKCVA 263
            KTF KV+FVYPK  ESVELM ++FDEENLP+EFGGKA L+Y +EE+S+ M QDD+K   
Sbjct: 122 AKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALLQYNYEEFSKQMNQDDVKTAN 181

Query: 264 FWEHG---EKQHHLVNGYSRAVVAPE 287
           FW  G     Q H  NG+S A +APE
Sbjct: 182 FWGLGHSNNNQLHASNGFSGAEIAPE 207

BLAST of HG10014031 vs. TAIR 10
Match: AT4G36640.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 298.1 bits (762), Expect = 7.4e-81
Identity = 134/224 (59.82%), Postives = 175/224 (78.12%), Query Frame = 0

Query: 63  MLEETFKWRATYKPEEIRWPEIASESETGKLYRASFHDREGRTILIMKPGKQNTTSIENQ 122
           M++ET KWR+TYKP+EIRW ++A E ETGK  RASFHDR+GR +LIM+P  QN+TS E  
Sbjct: 1   MIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGN 60

Query: 123 IRHLVYLMENALLNLPEGQEQMSWLIDFNGWSLNTSVPIKSARETVSILQNHYPERLALA 182
           IRHLVYL+ENA++NLP+GQ+QMSWLIDF GWS+  + P+K+ RE + ILQN+YPERL +A
Sbjct: 61  IRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIA 120

Query: 183 FLYNPPRIFEAFWKVVKYLLDPKTFQKVRFVYPKKQESVELMKSYFDEENLPSEFGGKAQ 242
           FLYNPPR+F+A ++  KY LDP+T +KV+FVYPK + S ELM ++FD ENLP EFGG+A 
Sbjct: 121 FLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEAT 180

Query: 243 LEYVHEEYSRLMIQDDIKCVAFWEHGEKQHHLVNGYSRAVVAPE 287
           LEY HE++SR M +DD+K   +W    K +   NG+S + V PE
Sbjct: 181 LEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPE 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906163.12.2e-15994.76CRAL-TRIO domain-containing protein C23B6.04c [Benincasa hispida] >XP_038906164.... [more]
XP_031738483.12.5e-15593.71CRAL-TRIO domain-containing protein C23B6.04c isoform X1 [Cucumis sativus][more]
XP_031738484.12.5e-15593.71CRAL-TRIO domain-containing protein C23B6.04c isoform X2 [Cucumis sativus][more]
XP_004147786.12.5e-15593.71CRAL-TRIO domain-containing protein C23B6.04c isoform X3 [Cucumis sativus] >XP_0... [more]
XP_008466570.14.3e-15594.06PREDICTED: CRAL-TRIO domain-containing protein C23B6.04c [Cucumis melo] >XP_0084... [more]
Match NameE-valueIdentityDescription
Q9UU997.6e-3033.06CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra... [more]
Q55CU81.9e-2528.74Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
P538444.3e-2532.32Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ... [more]
P538601.4e-2329.37Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ... [more]
P539897.4e-1730.12SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 376... [more]
Match NameE-valueIdentityDescription
A0A1S3CRQ62.1e-15594.06CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A5D3D8132.1e-15594.06CRAL-TRIO domain-containing protein C23B6.04c OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1J0L26.0e-15592.66CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1FJV07.9e-15592.31CRAL-TRIO domain-containing protein C23B6.04c isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1C7L81.3e-14687.06random slug protein 5 OS=Momordica charantia OX=3673 GN=LOC111008160 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G75170.14.6e-11567.36Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.24.6e-11567.36Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.18.7e-9858.12Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.39.4e-8468.93Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.27.4e-8159.82Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 42..67
e-value: 2.3E-8
score: 43.8
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 21..66
e-value: 1.5E-8
score: 34.7
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 87..242
e-value: 9.5E-52
score: 187.9
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 88..240
e-value: 7.7E-37
score: 126.5
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 81..245
score: 20.879837
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 99..240
e-value: 3.35433E-44
score: 145.606
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 5..274
e-value: 1.1E-81
score: 276.4
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 81..257
NoneNo IPR availablePANTHERPTHR45824GH16843Pcoord: 6..285
NoneNo IPR availablePANTHERPTHR45824:SF17DIVERGENT CRAL/TRIO DOMAIN PROTEINcoord: 6..285
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 10..83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10014031.1HG10014031.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0120009 intermembrane lipid transfer
biological_process GO:0015914 phospholipid transport
cellular_component GO:0016020 membrane
molecular_function GO:0008526 phosphatidylinositol transfer activity