HG10013346 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013346
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionNitrate reductase
LocationChr02: 643232 .. 643504 (-)
RNA-Seq ExpressionHG10013346
SyntenyHG10013346
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATGATATTTTTTTGAAGAAAGAGTTAGATGAGTGGAGTAAGAACGATAAGAGGTTTAAAGTATGGTATGTTGTAAGTGAGAGTGTAAGGGAAGGGTGGGAATTCAGTGGGAGTGATAAGGGAGACTATCATGGGGAACATCTTCCAGAAGATTCGGGGAATACGTTGGCCTTGGCTTATGGACCTCTGTCGATGATTAAGTTTGTTGTGCAACCCAACTTGGAGAAGATGAACTACGACACTACTAATTCGTGGTTGGTGTTTTAG

mRNA sequence

ATGGAGAATGATATTTTTTTGAAGAAAGAGTTAGATGAGTGGAGTAAGAACGATAAGAGGTTTAAAGTATGGTATGTTGTAAGTGAGAGTGTAAGGGAAGGGTGGGAATTCAGTGGGAGTGATAAGGGAGACTATCATGGGGAACATCTTCCAGAAGATTCGGGGAATACGTTGGCCTTGGCTTATGGACCTCTGTCGATGATTAAGTTTGTTGTGCAACCCAACTTGGAGAAGATGAACTACGACACTACTAATTCGTGGTTGGTGTTTTAG

Coding sequence (CDS)

ATGGAGAATGATATTTTTTTGAAGAAAGAGTTAGATGAGTGGAGTAAGAACGATAAGAGGTTTAAAGTATGGTATGTTGTAAGTGAGAGTGTAAGGGAAGGGTGGGAATTCAGTGGGAGTGATAAGGGAGACTATCATGGGGAACATCTTCCAGAAGATTCGGGGAATACGTTGGCCTTGGCTTATGGACCTCTGTCGATGATTAAGTTTGTTGTGCAACCCAACTTGGAGAAGATGAACTACGACACTACTAATTCGTGGTTGGTGTTTTAG

Protein sequence

MENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFSGSDKGDYHGEHLPEDSGNTLALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF
Homology
BLAST of HG10013346 vs. NCBI nr
Match: XP_008444121.1 (PREDICTED: nitrate reductase [NADH]-like [Cucumis melo] >KAA0064285.1 nitrate reductase [Cucumis melo var. makuwa] >TYK01021.1 nitrate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-26
Identity = 66/90 (73.33%), Postives = 69/90 (76.67%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+AL
Sbjct: 802 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVTESIMREHLPEASSETMAL 861

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT NS LVF
Sbjct: 862 ACGPPPMIKFAVQPNLEKMNYDTNNSLLVF 891

BLAST of HG10013346 vs. NCBI nr
Match: KAE8649633.1 (hypothetical protein Csa_012045 [Cucumis sativus])

HSP 1 Score: 128.3 bits (321), Expect = 3.4e-26
Identity = 65/90 (72.22%), Postives = 71/90 (78.89%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+AL
Sbjct: 846 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMAL 905

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF V+P+LEKMNYDTTNS LVF
Sbjct: 906 ACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 935

BLAST of HG10013346 vs. NCBI nr
Match: XP_038897076.1 (nitrate reductase [NADH]-like [Benincasa hispida])

HSP 1 Score: 128.3 bits (321), Expect = 3.4e-26
Identity = 65/90 (72.22%), Postives = 72/90 (80.00%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI LK+ELDEW+K+DKRFKVWYVV+ESVREGW++S G        EHLPE S  TLAL
Sbjct: 807 EDDILLKEELDEWTKSDKRFKVWYVVNESVREGWKYSVGMVTESIMREHLPEASPETLAL 866

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDTTNS LVF
Sbjct: 867 ACGPPPMIKFAVQPNLEKMNYDTTNSLLVF 896

BLAST of HG10013346 vs. NCBI nr
Match: XP_011652901.1 (uncharacterized protein LOC101216001 isoform X1 [Cucumis sativus])

HSP 1 Score: 128.3 bits (321), Expect = 3.4e-26
Identity = 65/90 (72.22%), Postives = 71/90 (78.89%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+AL
Sbjct: 804 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMAL 863

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF V+P+LEKMNYDTTNS LVF
Sbjct: 864 ACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 893

BLAST of HG10013346 vs. NCBI nr
Match: KAG7024645.1 (hypothetical protein SDJN02_13463, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.9 bits (302), Expect = 5.5e-24
Identity = 60/90 (66.67%), Postives = 69/90 (76.67%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI +++ELDEWS++DKRF VWYVV ESVREGWE+S G        EHLP  S ++LAL
Sbjct: 842 ENDILMREELDEWSRSDKRFNVWYVVQESVREGWEYSVGVVSESIMREHLPAASADSLAL 901

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT +S LVF
Sbjct: 902 ACGPPPMIKFAVQPNLEKMNYDTADSLLVF 931

BLAST of HG10013346 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.7e-24
Identity = 55/90 (61.11%), Postives = 68/90 (75.56%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI L+ ELD W+K ++R KVWYVV ES+REGWE+S G    +   EH+P  + +TLAL
Sbjct: 829 EDDILLRDELDTWAKKNQRLKVWYVVQESIREGWEYSVGFITENILREHIPAAAEDTLAL 888

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP +MI+F VQPNLEKMNYDT NS LVF
Sbjct: 889 ACGPPAMIQFAVQPNLEKMNYDTKNSLLVF 918

BLAST of HG10013346 vs. ExPASy Swiss-Prot
Match: P54233 (Inducible nitrate reductase [NADH] 1 OS=Glycine max OX=3847 GN=INR1 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.1e-22
Identity = 53/90 (58.89%), Postives = 64/90 (71.11%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI LK+ELDEW+K   R KVWYV+ ES+REGWE+S G        EH+P  S +TLAL
Sbjct: 797 EDDILLKEELDEWAKKYDRLKVWYVIQESIREGWEYSVGFITESILTEHIPNASPDTLAL 856

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
             GP  MI+F VQPNLEK+ YDT N+ LVF
Sbjct: 857 TCGPPPMIQFAVQPNLEKLGYDTQNNLLVF 886

BLAST of HG10013346 vs. ExPASy Swiss-Prot
Match: P39870 (Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 5.3e-22
Identity = 52/90 (57.78%), Postives = 63/90 (70.00%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI LK+ELDEW+K   R KVWYV+  S+REGWE+S G        EH+P  S +TLAL
Sbjct: 801 EDDILLKEELDEWAKKYDRLKVWYVIQASIREGWEYSVGFITESILTEHIPNASPDTLAL 860

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
             GP  MI+F VQPNLEK+ YDT N+ LVF
Sbjct: 861 TCGPPPMIQFAVQPNLEKLGYDTQNNLLVF 890

BLAST of HG10013346 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.0e-20
Identity = 50/90 (55.56%), Postives = 65/90 (72.22%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI L++ELD+W+K  ++ KVWYVV ES REGWE+S G  +     EH+PE S + LAL
Sbjct: 809 EDDILLREELDDWAKKHEKLKVWYVVKESKREGWEYSVGYIRESILREHIPEGSDDVLAL 868

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A G  SMI+  V+ NLEKMNYDT NS ++F
Sbjct: 869 ACGAPSMIEEAVRLNLEKMNYDTKNSLIIF 898

BLAST of HG10013346 vs. ExPASy Swiss-Prot
Match: P43101 (Nitrate reductase [NADH] OS=Cichorium intybus OX=13427 GN=NIA PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.7e-20
Identity = 52/91 (57.14%), Postives = 65/91 (71.43%), Query Frame = 0

Query: 2   ENDIFLKKELDEWS-KNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLA 61
           E+DI L++ELD W+ K   R KVWYVV++S+REGW++S G    D   EH+PE S +TLA
Sbjct: 830 EDDILLREELDAWADKYSDRVKVWYVVAKSIREGWKYSEGFITEDIMREHVPEVSEDTLA 889

Query: 62  LAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           LA GP  MI+F + PNLEKM YD  NS LVF
Sbjct: 890 LACGPPPMIQFAINPNLEKMGYDIKNSLLVF 920

BLAST of HG10013346 vs. ExPASy TrEMBL
Match: A0A5A7VEG1 (Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00230 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.5e-27
Identity = 66/90 (73.33%), Postives = 69/90 (76.67%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+AL
Sbjct: 802 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVTESIMREHLPEASSETMAL 861

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT NS LVF
Sbjct: 862 ACGPPPMIKFAVQPNLEKMNYDTNNSLLVF 891

BLAST of HG10013346 vs. ExPASy TrEMBL
Match: A0A1S3BAF3 (Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103487560 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.5e-27
Identity = 66/90 (73.33%), Postives = 69/90 (76.67%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G        EHLPE S  T+AL
Sbjct: 802 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVTESIMREHLPEASSETMAL 861

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT NS LVF
Sbjct: 862 ACGPPPMIKFAVQPNLEKMNYDTNNSLLVF 891

BLAST of HG10013346 vs. ExPASy TrEMBL
Match: A0A0A0L1Y7 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G377160 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-26
Identity = 65/90 (72.22%), Postives = 71/90 (78.89%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI LK ELDEWSK DKRFKVWYVV+ESVREGWE+S G  +     EHLPE S  T+AL
Sbjct: 804 ENDILLKDELDEWSKRDKRFKVWYVVNESVREGWEYSVGVVRESIMREHLPEASPETMAL 863

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF V+P+LEKMNYDTTNS LVF
Sbjct: 864 ACGPPPMIKFAVKPSLEKMNYDTTNSLLVF 893

BLAST of HG10013346 vs. ExPASy TrEMBL
Match: A0A6J1C1V3 (nitrate reductase [NADH]-like OS=Momordica charantia OX=3673 GN=LOC111007507 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.7e-24
Identity = 61/90 (67.78%), Postives = 70/90 (77.78%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           E+DI L++E+DEW K+DKRFKVWYVV ESVREGWEFS G        EHLP+ S +TLAL
Sbjct: 174 EDDILLREEMDEWVKSDKRFKVWYVVKESVREGWEFSVGVVTESIMREHLPKASVDTLAL 233

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT +S L+F
Sbjct: 234 ACGPPPMIKFAVQPNLEKMNYDTESSLLIF 263

BLAST of HG10013346 vs. ExPASy TrEMBL
Match: A0A6J1F6X9 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111442711 PE=3 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.7e-24
Identity = 60/90 (66.67%), Postives = 67/90 (74.44%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPEDSGNTLAL 61
           ENDI ++ ELDEWS++DKRF VWYVV ESVREGWE+S G        EHLP  S + LAL
Sbjct: 798 ENDILMRDELDEWSRSDKRFNVWYVVQESVREGWEYSVGVVSESIMREHLPAASADLLAL 857

Query: 62  AYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A GP  MIKF VQPNLEKMNYDT +S LVF
Sbjct: 858 ACGPPPMIKFAVQPNLEKMNYDTADSLLVF 887

BLAST of HG10013346 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 80.5 bits (197), Expect = 7.7e-16
Identity = 45/92 (48.91%), Postives = 59/92 (64.13%), Query Frame = 0

Query: 2   ENDIFLKKELDEWSKN-DKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTL 61
           E DI L++ELD W++    R KVWYVV ES +EGW +S G        EH+P+   G+ L
Sbjct: 827 EEDILLREELDGWAEQYPDRLKVWYVV-ESAKEGWAYSTGFISEAIMREHIPDGLDGSAL 886

Query: 62  ALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           A+A GP  MI+F VQPNLEKM Y+    +L+F
Sbjct: 887 AMACGPPPMIQFAVQPNLEKMQYNIKEDFLIF 917

BLAST of HG10013346 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 79.0 bits (193), Expect = 2.2e-15
Identity = 44/92 (47.83%), Postives = 60/92 (65.22%), Query Frame = 0

Query: 2   ENDIFLKKELDEW-SKNDKRFKVWYVVSESVREGWEFS-GSDKGDYHGEHLPED-SGNTL 61
           E+DI +++EL+ W SK+ +R K+WYVV E  +EGW +S G        EH+PE   G +L
Sbjct: 827 EDDILVREELEGWASKHKERLKIWYVV-EIAKEGWSYSTGFITEAVLREHIPEGLEGESL 886

Query: 62  ALAYGPLSMIKFVVQPNLEKMNYDTTNSWLVF 91
           ALA GP  MI+F +QPNLEKM Y+     L+F
Sbjct: 887 ALACGPPPMIQFALQPNLEKMGYNVKEDLLIF 917

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444121.11.5e-2673.33PREDICTED: nitrate reductase [NADH]-like [Cucumis melo] >KAA0064285.1 nitrate re... [more]
KAE8649633.13.4e-2672.22hypothetical protein Csa_012045 [Cucumis sativus][more]
XP_038897076.13.4e-2672.22nitrate reductase [NADH]-like [Benincasa hispida][more]
XP_011652901.13.4e-2672.22uncharacterized protein LOC101216001 isoform X1 [Cucumis sativus][more]
KAG7024645.15.5e-2466.67hypothetical protein SDJN02_13463, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
P175695.7e-2461.11Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1[more]
P542331.1e-2258.89Inducible nitrate reductase [NADH] 1 OS=Glycine max OX=3847 GN=INR1 PE=2 SV=1[more]
P398705.3e-2257.78Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1[more]
P277831.0e-2055.56Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1[more]
P431011.7e-2057.14Nitrate reductase [NADH] OS=Cichorium intybus OX=13427 GN=NIA PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7VEG17.5e-2773.33Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00... [more]
A0A1S3BAF37.5e-2773.33Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103487560 PE=3 SV=1[more]
A0A0A0L1Y71.7e-2672.22Nitrate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G377160 PE=3 SV=1[more]
A0A6J1C1V32.7e-2467.78nitrate reductase [NADH]-like OS=Momordica charantia OX=3673 GN=LOC111007507 PE=... [more]
A0A6J1F6X97.7e-2466.67Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111442711 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G37130.17.7e-1648.91nitrate reductase 2 [more]
AT1G77760.12.2e-1547.83nitrate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 2..72
e-value: 4.1E-6
score: 27.4
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 1..90
e-value: 7.8E-13
score: 50.3
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 2..85
NoneNo IPR availablePANTHERPTHR19370:SF198NITRATE REDUCTASEcoord: 2..90
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 2..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013346.1HG10013346.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
molecular_function GO:0071949 FAD binding
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity