HG10013312 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10013312
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	aspartic proteinase Asp1-like
Location	Chr02: 309455 .. 311542 (-)
RNA-Seq Expression	HG10013312
Synteny	HG10013312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATACCTTGAATCTGTCGCCTGTCTTCGTTCTCTTTGCCATTTTCGCTGTTTTCTTGTGCGACAAGGTTTGCCTCGCCGTCCGGAAGTCATCGGCGACCAATGCTTTTGATTCGTCGATCGTCTTTCCCGTCAAAGGGAATGTTTACCCTCTAGGGTTTGTGTTTCTACTTTCTTTCTTATTCGATCTTTGATTTTGATTCTCTTTTTCTCTCGTTTTGTTTGGATTATTATTTTTGATAGATTGTTGAAATTTCCTCGATTATGGGTTGGAATCTGCAGGCATTTTACGGTTTCCGTTACTATTGGCAATCCGCCGAAGGTTTTTGAACTGGATATCGACACTGGGAGTGACCTCACTTGGGTCCAATGTGACGCTCCATGTACTGGCTGCACTCTGGTCAGTTGGATTCAAATCGAGTTTTATCCGATAATTTATTTCTTCTCTTTTGATTTCGAAGAGTTGATTCTGAATTGTAGCGTAATTTTGTGTCATTTATTACTTTTGTAGCCTCGCGATCGACTCTATAAACCGCACAAGAACGTTGTGCGTTGTGGAGAACCATTGTGTTCAGCACTTTTCTCCGCAGACAAGTCCCCTTGTAAGAACCCTAATGATCAATGTGACTACGAGGTTGAGTATGCTGACCATGGATCATCCATTGGTGTATTGGTCAAAGATCCTGTTCCCTTGAGACTCACTAATGGCACTGTCTTAGCTCCCAATTTGGGCTTTGGGTTTGTATATAATAACATACGAGTTTTCTTTGACATTGCTCATTCTTTTGGCAGTTTTCATTGCTTGATTTTGGCCTGCCAACAGTAAAGGAAACTTAGTACCAAACTTTCTTTCCTATGATTGTATTGTTTACAGTTGTGGATATGATCAGCATAATGGCGGTTCACAATTGCCTCCGACGGCTGGTGTCCTTGGGCTTGGAAATAGTAAAGCTACCATGGCAACACAGTTAAGCACCCTCAGTAATGTACGCAACATAATTGGCCACTGCTTCAGCGGACAAGGGGGAGGATTTCTATTCTTTGGAGGAAACCTTGTTCCATCTTCAGGGATGTTGTGGACGCCGATATTACGCACTCCGGGAGGGTCAGTAAGAAGCCTCCTTGAGCAATTCTGATAACTTTTGACATTGATTAGTGCTTCTTAAAGCTTTGTGTAGTTGTGACTGAAATAGTGTTTGATGTCAATCTCTCAGAAAGTACTCAGCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGCCTGTCGGTATTAGAGGCGTTATACTAACTTTTGACAGTGGAAGCTCCTATACTTACTTCAACAATCAAGTTTATGGAGCAGTACTCAATCTGGTGAGTTAGTGACAAGAAATTCTTTTAATAGTTATTAATGTGTTGAATGAGATATCATCAGAAGTAGGAGTTTGTAGGAAGAAACTAATAATCTGAAATGATATCCCAGTTGAGGAATGGTTTGAAAGGACAACCATTAAAAGATGCACCTGAAGATAAGACCCTTCCAATATGCTGGAAAGGCTCAAAAGCTTTCAAATCAGTGGCTGATGTGAGAAACTTTTTCAAGCCCTTGGCCTTGAGCTTTACAAACTCCAAGAATGTTCAGTTTCAAATACCACCTGAAGCTTATCTAATTATTAGTGTGAGTTTCCCAGACATCATGTTTGTTTATCCATAATTAACTGAATGAAACCATATGTTACTAACAAAATATAACACTCGTAAGCAAAAGTGAGCATAGATCAGTGACATGTACTCCTTCCTCTTGAAGTTGGAGTTGCACTAAGATTTTAACAAGCTCTTTTTACTTTTACAGAATTTGGGTAATGTTTGCTTGGGAATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAACCTGATTGGAGGTAAGATATTGAGAAACACTGTTGAATCTTTCTTTCACTTGTTTTCTTCCATCATTTCAACTTTCATTTTCCCCCCAATATTCCTGTACAGATATCTCCTTGATTGATAAAATGGTGGTCTATGACAACGAGAGGCAGCAGATCGGTTGGGCTCCTGCAAACTGCAGTAAGCCTCCCAAGAAATGA

mRNA sequence

ATGCATACCTTGAATCTGTCGCCTGTCTTCGTTCTCTTTGCCATTTTCGCTGTTTTCTTGTGCGACAAGGTTTGCCTCGCCGTCCGGAAGTCATCGGCGACCAATGCTTTTGATTCGTCGATCGTCTTTCCCGTCAAAGGGAATGTTTACCCTCTAGGGCATTTTACGGTTTCCGTTACTATTGGCAATCCGCCGAAGGTTTTTGAACTGGATATCGACACTGGGAGTGACCTCACTTGGGTCCAATGTGACGCTCCATGTACTGGCTGCACTCTGCCTCGCGATCGACTCTATAAACCGCACAAGAACGTTGTGCGTTGTGGAGAACCATTGTGTTCAGCACTTTTCTCCGCAGACAAGTCCCCTTGTAAGAACCCTAATGATCAATGTGACTACGAGGTTGAGTATGCTGACCATGGATCATCCATTGGTGTATTGGTCAAAGATCCTGTTCCCTTGAGACTCACTAATGGCACTGTCTTAGCTCCCAATTTGGGCTTTGGTTGTGGATATGATCAGCATAATGGCGGTTCACAATTGCCTCCGACGGCTGGTGTCCTTGGGCTTGGAAATAGTAAAGCTACCATGGCAACACAGTTAAGCACCCTCAGTAATGTACGCAACATAATTGGCCACTGCTTCAGCGGACAAGGGGGAGGATTTCTATTCTTTGGAGGAAACCTTGTTCCATCTTCAGGGATGTTGTGGACGCCGATATTACGCACTCCGGGAGGAAAGTACTCAGCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGCCTGTCGGTATTAGAGGCGTTATACTAACTTTTGACAGTGGAAGCTCCTATACTTACTTCAACAATCAAGTTTATGGAGCAGTACTCAATCTGTTGAGGAATGGTTTGAAAGGACAACCATTAAAAGATGCACCTGAAGATAAGACCCTTCCAATATGCTGGAAAGGCTCAAAAGCTTTCAAATCAGTGGCTGATGTGAGAAACTTTTTCAAGCCCTTGGCCTTGAGCTTTACAAACTCCAAGAATGTTCAGTTTCAAATACCACCTGAAGCTTATCTAATTATTAGTAATTTGGGTAATGTTTGCTTGGGAATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAACCTGATTGGAGATATCTCCTTGATTGATAAAATGGTGGTCTATGACAACGAGAGGCAGCAGATCGGTTGGGCTCCTGCAAACTGCAGTAAGCCTCCCAAGAAATGA

Coding sequence (CDS)

ATGCATACCTTGAATCTGTCGCCTGTCTTCGTTCTCTTTGCCATTTTCGCTGTTTTCTTGTGCGACAAGGTTTGCCTCGCCGTCCGGAAGTCATCGGCGACCAATGCTTTTGATTCGTCGATCGTCTTTCCCGTCAAAGGGAATGTTTACCCTCTAGGGCATTTTACGGTTTCCGTTACTATTGGCAATCCGCCGAAGGTTTTTGAACTGGATATCGACACTGGGAGTGACCTCACTTGGGTCCAATGTGACGCTCCATGTACTGGCTGCACTCTGCCTCGCGATCGACTCTATAAACCGCACAAGAACGTTGTGCGTTGTGGAGAACCATTGTGTTCAGCACTTTTCTCCGCAGACAAGTCCCCTTGTAAGAACCCTAATGATCAATGTGACTACGAGGTTGAGTATGCTGACCATGGATCATCCATTGGTGTATTGGTCAAAGATCCTGTTCCCTTGAGACTCACTAATGGCACTGTCTTAGCTCCCAATTTGGGCTTTGGTTGTGGATATGATCAGCATAATGGCGGTTCACAATTGCCTCCGACGGCTGGTGTCCTTGGGCTTGGAAATAGTAAAGCTACCATGGCAACACAGTTAAGCACCCTCAGTAATGTACGCAACATAATTGGCCACTGCTTCAGCGGACAAGGGGGAGGATTTCTATTCTTTGGAGGAAACCTTGTTCCATCTTCAGGGATGTTGTGGACGCCGATATTACGCACTCCGGGAGGAAAGTACTCAGCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGCCTGTCGGTATTAGAGGCGTTATACTAACTTTTGACAGTGGAAGCTCCTATACTTACTTCAACAATCAAGTTTATGGAGCAGTACTCAATCTGTTGAGGAATGGTTTGAAAGGACAACCATTAAAAGATGCACCTGAAGATAAGACCCTTCCAATATGCTGGAAAGGCTCAAAAGCTTTCAAATCAGTGGCTGATGTGAGAAACTTTTTCAAGCCCTTGGCCTTGAGCTTTACAAACTCCAAGAATGTTCAGTTTCAAATACCACCTGAAGCTTATCTAATTATTAGTAATTTGGGTAATGTTTGCTTGGGAATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAACCTGATTGGAGATATCTCCTTGATTGATAAAATGGTGGTCTATGACAACGAGAGGCAGCAGATCGGTTGGGCTCCTGCAAACTGCAGTAAGCCTCCCAAGAAATGA

Protein sequence

MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQLPPTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK

Homology

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BLAST of HG10013312 vs. NCBI nr
Match: XP_008444185.1 (PREDICTED: aspartic proteinase Asp1-like [Cucumis melo] >TYK30005.1 aspartic proteinase Asp1-like [Cucumis melo var. makuwa])

HSP 1 Score: 775.0 bits (2000), Expect = 3.2e-220
Identity = 369/411 (89.78%), Postives = 386/411 (93.92%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           M   NLSP+F+LF IFAVFLC   CLA  KS A N  DSSI+FPVKGNVYPLGHFTVSVT
Sbjct: 1   MPAFNLSPLFLLFVIFAVFLCGTFCLADWKSPAANPLDSSILFPVKGNVYPLGHFTVSVT 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPH NVVRCGEPLC+ALFSA K
Sbjct: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHNNVVRCGEPLCAALFSASK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
           SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGT+LAPNLGFGCGYDQHNGGSQ 
Sbjct: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGSQS 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP TAGVLGLGNSKATMATQLS LS+VRN++GHCFSGQG GFLFFGG+LVPSSGM W PI
Sbjct: 181 PPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGDGFLFFGGDLVPSSGMSWMPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           LRTPGGKYSAGPAEVYFGG PVGIRG+ILTFDSGSSYTYFN+QVYGAVLNLLRNGLKGQP
Sbjct: 241 LRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           L+DAPEDKTLP+CWKGSKAFKSVAD RNFFKPLALSF NSK VQFQIPPEAYLIISNLGN
Sbjct: 301 LRDAPEDKTLPMCWKGSKAFKSVADARNFFKPLALSFGNSKKVQFQIPPEAYLIISNLGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGILNGSQVGLGNVNLIGDIS++DKM+VYDNERQQIGWAPANCS+PPKK
Sbjct: 361 VCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSRPPKK 411

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BLAST of HG10013312 vs. NCBI nr
Match: XP_004142705.1 (aspartic proteinase Asp1 [Cucumis sativus])

HSP 1 Score: 772.7 bits (1994), Expect = 1.6e-219
Identity = 370/411 (90.02%), Postives = 387/411 (94.16%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           MH  NLSP+ +LF IF++  C   CLA  KSSA N FDSSI+ PVKGNVYPLGHFTVSVT
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLADWKSSAVNPFDSSILLPVKGNVYPLGHFTVSVT 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLP DRLYKPH NVVRCGEPLCSALFSA K
Sbjct: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSASK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
           SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGT+LAPNLGFGCGYDQHNGGSQL
Sbjct: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGSQL 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP TAGVLGLGNSKATMATQLS LS+VRN++GHCFSGQGGGFLFFGG+LVPSSGM W PI
Sbjct: 181 PPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWMPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           LRTPGGKYSAGPAEVYFGG PVGIRG+ILTFDSGSSYTYFN+QVYGAVLNLLRNGLKGQP
Sbjct: 241 LRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           L+DAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSF NSK VQFQIPPEAYLIISNLGN
Sbjct: 301 LRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNLGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGILNGSQVGLGNVNLIGDIS++DKM+VYDNERQQIGWAPANCSKPP+K
Sbjct: 361 VCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

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BLAST of HG10013312 vs. NCBI nr
Match: KAG6591878.1 (Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 325/411 (79.08%), Postives = 368/411 (89.54%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           M   NL PVFVLFA F + L  K+C A RK S  N   SSIVFPVKGNVYPLGHFT SV 
Sbjct: 1   MRMSNLFPVFVLFAAFVLSLSGKLCFADRKPSVINRPSSSIVFPVKGNVYPLGHFTASVN 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           +GNPPKVF+LDIDTGSD+TWVQCDAPCTGCTLPRD+LYKPH NVVRCGEPLC+ALF   K
Sbjct: 61  VGNPPKVFDLDIDTGSDVTWVQCDAPCTGCTLPRDKLYKPHNNVVRCGEPLCAALFHQGK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
            PC+NPNDQCDY++EYADHGSSIGVLVKD VP++L NGTV+APNLGFGCGYDQHNGGSQ 
Sbjct: 121 PPCRNPNDQCDYQIEYADHGSSIGVLVKDLVPMKLANGTVIAPNLGFGCGYDQHNGGSQP 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP T GVLGLGNSK T+A+Q+S+L++VR++IGHC+SG GGGFLFFGG+LVPSSG+ WTPI
Sbjct: 181 PPSTGGVLGLGNSKGTLASQISSLTHVRSVIGHCYSGHGGGFLFFGGDLVPSSGISWTPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           L T GG+YS+GPA+V+FGGK VGIRG+ LTFDSGSSYTYFN+QVYGA+LN LRN LKGQP
Sbjct: 241 LHTSGGRYSSGPADVFFGGKAVGIRGLTLTFDSGSSYTYFNSQVYGAILNTLRNDLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           LKDAPE+K LP+CWKGSKAFKSVADVR+FFKPLALSFTNS+N QFQ+PPE+YLIIS LGN
Sbjct: 301 LKDAPEEKILPVCWKGSKAFKSVADVRSFFKPLALSFTNSRNAQFQMPPESYLIISELGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGIL+GSQVGLGNVNLIGDIS +DK+VVYDNE+QQIGWAPANCS+P K+
Sbjct: 361 VCLGILDGSQVGLGNVNLIGDISFLDKIVVYDNEKQQIGWAPANCSRPRKQ 411

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BLAST of HG10013312 vs. NCBI nr
Match: XP_022132194.1 (aspartic proteinase Asp1-like isoform X4 [Momordica charantia])

HSP 1 Score: 680.6 bits (1755), Expect = 8.2e-192
Identity = 320/412 (77.67%), Postives = 359/412 (87.14%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCL--AVRKSSATNAFDSSIVFPVKGNVYPLGHFTVS 60
           MH+  L PV +LFAIFAV   DK  L   ++  S +N F SS+ FP+KGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSA 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLPRD+ Y+PH N VRC EPLCSALF  
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 DKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D  P+RLTNG+V APNLGFGCGYDQ +GGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 QLPPTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTP 240
               TAGVLGLG+ KATM +QLS L +VRN++GHC SGQGGGFLFFGG+LVPSSGM WTP
Sbjct: 181 ----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTP 240

Query: 241 ILRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQ 300
           I+ +PGG+YS+GPAEVYFGGK  GIRG+ LTFDSGSSYTYFN+QVYGAVLNLL+N LKG+
Sbjct: 241 IMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGK 300

Query: 301 PLKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLG 360
           PL D P+DKTLP+CWKG+KAF+SV DVRNFFKPLALSFT+SKNVQFQIPPEAYLIIS  G
Sbjct: 301 PLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFG 360

Query: 361 NVCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           NVC GILNGSQVGLGNVN+IGDISL+DK+VVYDNERQQIGWAPANC++PPKK
Sbjct: 361 NVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

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BLAST of HG10013312 vs. NCBI nr
Match: XP_022132187.1 (aspartic proteinase Asp1-like isoform X3 [Momordica charantia])

HSP 1 Score: 665.6 bits (1716), Expect = 2.7e-187
Identity = 320/440 (72.73%), Postives = 359/440 (81.59%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCL--AVRKSSATNAFDSSIVFPVKGNVYPLGHFTVS 60
           MH+  L PV +LFAIFAV   DK  L   ++  S +N F SS+ FP+KGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDRLYKPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDP 180
             PRD+ Y+PH N VRC EPLCSALF   K PCKNPNDQCDY+VEYAD GSSIGVLV+D 
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPLRLTNGTVLAPNLGFGCGYDQHNGGSQLPPTAGVLGLGNSKATMATQLSTLSNVRNII 240
            P+RLTNG+V APNLGFGCGYDQ +GGS    TAGVLGLG+ KATM +QLS L +VRN++
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGS----TAGVLGLGSGKATMTSQLSALGHVRNVV 240

Query: 241 GHCFSGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGGKPVGIRGVILTF 300
           GHC SGQGGGFLFFGG+LVPSSGM WTPI+ +PGG+YS+GPAEVYFGGK  GIRG+ LTF
Sbjct: 241 GHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTF 300

Query: 301 DSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVRNFFK 360
           DSGSSYTYFN+QVYGAVLNLL+N LKG+PL D P+DKTLP+CWKG+KAF+SV DVRNFFK
Sbjct: 301 DSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFK 360

Query: 361 PLALSFTNSKNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNLIGDISLIDKMVVY 411
           PLALSFT+SKNVQFQIPPEAYLIIS  GNVC GILNGSQVGLGNVN+IGDISL+DK+VVY
Sbjct: 361 PLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVY 420

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BLAST of HG10013312 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 9.4e-82
Identity = 163/383 (42.56%), Postives = 236/383 (61.62%), Query Frame = 0


Query: 39  SSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLY 98
           S++V  + GNVYP+GHF +++ IG+P K + LDIDTGS LTW+QCDAPCT C +    LY
Sbjct: 22  SAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVLY 81

Query: 99  KP-HKNVVRCGEPLCSALFSADKSPCK-NPNDQCDYEVEYADHGSSIGVLVKDPVPLRLT 158
           KP  K +V C + LC+ L++    P +     QCDY ++Y D  SS+GVLV D   L  +
Sbjct: 82  KPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSAS 141

Query: 159 NGTVLAPNLGFGCGYDQHNGGSQLP-PTAGVLGLGNSKATMATQLSTLSNV-RNIIGHCF 218
           NGT     + FGCGYDQ      +P P   +LGL   K T+ +QL +   + ++++GHC 
Sbjct: 142 NGT-NPTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 219 SGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYF--GGKPVGIRGVILTFDS 278
           S +GGGFLFFG   VP+SG+ WTP+ R     YS G   ++F    K +    + + FDS
Sbjct: 202 SSKGGGFLFFGDAQVPTSGVTWTPMNR-EHKYYSPGHGTLHFDSNSKAISAAPMAVIFDS 261

Query: 279 GSSYTYFNNQVYGAVLNLLRNGLKGQP--LKDAPE-DKTLPICWKGSKAFKSVADVRNFF 338
           G++YTYF  Q Y A L+++++ L  +   L +  E D+ L +CWKG     ++ +V+  F
Sbjct: 262 GATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKCF 321

Query: 339 KPLALSFTN-SKNVQFQIPPEAYLIISNLGNVCLGILNGSQ--VGLGNVNLIGDISLIDK 398
           + L+L F +  K    +IPPE YLIIS  G+VCLGIL+GS+  + L   NLIG I+++D+
Sbjct: 322 RSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLDQ 381

Query: 399 MVVYDNERQQIGWAPANCSKPPK 410
           MV+YD+ER  +GW    C + P+
Sbjct: 382 MVIYDSERSLLGWVNYQCDRIPR 401

[Close]

BLAST of HG10013312 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.7e-81
Identity = 170/400 (42.50%), Postives = 243/400 (60.75%), Query Frame = 0


Query: 28  VRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVTIGNPP--KVFELDIDTGSDLTWVQCDA 87
           V  +SA +   S+ +FPV GNVYP G +   + +G P   + + LDIDTGS+LTW+QCDA
Sbjct: 176 VLSTSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDA 235

Query: 88  PCTGCTLPRDRLYKPHK-NVVRCGEPLCSALFSADKSP-CKNPNDQCDYEVEYADHGSSI 147
           PCT C    ++LYKP K N+VR  E  C  +     +  C+N + QCDYE+EYADH  S+
Sbjct: 236 PCTSCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCH-QCDYEIEYADHSYSM 295

Query: 148 GVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHN-GGSQLPPTAGVLGLGNSKATMATQLST 207
           GVL KD   L+L NG++   ++ FGCGYDQ     + L  T G+LGL  +K ++ +QL++
Sbjct: 296 GVLTKDKFHLKLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLAS 355

Query: 208 LSNVRNIIGHCFSG--QGGGFLFFGGNLVPSSGMLWTPIL--------RTPGGKYSAGPA 267
              + N++GHC +    G G++F G +LVPS GM W P+L        +    K S G  
Sbjct: 356 RGIISNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQG 415

Query: 268 EVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPIC 327
            +   G+  G  G +L FD+GSSYTYF NQ Y  ++  L+  + G  L     D+TLPIC
Sbjct: 416 MLSLDGEN-GRVGKVL-FDTGSSYTYFPNQAYSQLVTSLQE-VSGLELTRDDSDETLPIC 475

Query: 328 W--KGSKAFKSVADVRNFFKPLALSFTNSKNV---QFQIPPEAYLIISNLGNVCLGILNG 387
           W  K +  F S++DV+ FF+P+ L   +   +   +  I PE YLIISN GNVCLGIL+G
Sbjct: 476 WRAKTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDG 535

Query: 388 SQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKP 408
           S V  G+  ++GDIS+   ++VYDN +++IGW  ++C +P
Sbjct: 536 SSVHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVRP 571

[Close]

BLAST of HG10013312 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 1.4e-80
Identity = 162/383 (42.30%), Postives = 235/383 (61.36%), Query Frame = 0


Query: 39  SSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLY 98
           S++V  + GNVYP+GHF V++ IG+P K + LDIDTGS LTW+QCD PC  C      LY
Sbjct: 22  SAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGLY 81

Query: 99  KPH-KNVVRCGEPLCSALFSADKSPCK-NPNDQCDYEVEYADHGSSIGVLVKDPVPLRLT 158
           KP  K  V+C E  C+ L++  + P K  P +QC Y ++Y   GSSIGVL+ D   L  +
Sbjct: 82  KPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPAS 141

Query: 159 NGTVLAPNLGFGCGYDQHNGGSQLP-PTAGVLGLGNSKATMATQLSTLSNV-RNIIGHCF 218
           NGT    ++ FGCGY+Q      +P P  G+LGLG  K T+ +QL +   + ++++GHC 
Sbjct: 142 NGT-NPTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 219 SGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYF--GGKPVGIRGVILTFDS 278
           S +G GFLFFG   VP+SG+ W+P+ R     YS     + F    KP+    + + FDS
Sbjct: 202 SSKGKGFLFFGDAKVPTSGVTWSPMNR-EHKHYSPRQGTLQFNSNSKPISAAPMEVIFDS 261

Query: 279 GSSYTYFNNQVYGAVLNLLRNGLKGQP--LKDAPE-DKTLPICWKGSKAFKSVADVRNFF 338
           G++YTYF  Q Y A L+++++ L  +   L +  E D+ L +CWKG    +++ +V+  F
Sbjct: 262 GATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKCF 321

Query: 339 KPLALSFTN-SKNVQFQIPPEAYLIISNLGNVCLGILNGSQ--VGLGNVNLIGDISLIDK 398
           + L+L F +  K    +IPPE YLIIS  G+VCLGIL+GS+    L   NLIG I+++D+
Sbjct: 322 RSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLDQ 381

Query: 399 MVVYDNERQQIGWAPANCSKPPK 410
           MV+YD+ER  +GW    C + P+
Sbjct: 382 MVIYDSERSLLGWVNYQCDRIPR 401

[Close]

BLAST of HG10013312 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.0e-31
Identity = 110/406 (27.09%), Postives = 177/406 (43.60%), Query Frame = 0


Query: 39  SSIVFPVKGN--VYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDR 98
           +SI  P+ G+  V  +G +   + +G+PPK + + +DTGSD+ W+ C  PC  C    + 
Sbjct: 56  ASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPTKTNL 115

Query: 99  LYK---------PHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLV 158
            ++              V C +  CS +  +D      P   C Y + YAD  +S G  +
Sbjct: 116 NFRLSLFDMNASSTSKKVGCDDDFCSFISQSDSC---QPALGCSYHIVYADESTSDGKFI 175

Query: 159 KDPVPLRLTNGTVLAPNLG----FGCGYDQH----NGGSQLPPTAGVLGLGNSKATMATQ 218
           +D + L    G +    LG    FGCG DQ     NG S +    GV+G G S  ++ +Q
Sbjct: 176 RDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVD---GVMGFGQSNTSVLSQ 235

Query: 219 LSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGGK 278
           L+   + + +  HC     GG +F  G +V S  +  TP++          P ++++   
Sbjct: 236 LAATGDAKRVFSHCLDNVKGGGIFAVG-VVDSPKVKTTPMV----------PNQMHYNVM 295

Query: 279 PVG--------------IRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPE 338
            +G              +R      DSG++  YF   +Y +++  +   L  QP+K    
Sbjct: 296 LMGMDVDGTSLDLPRSIVRNGGTIVDSGTTLAYFPKVLYDSLIETI---LARQPVK---- 355

Query: 339 DKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGNVCLGIL 398
              L I  +  + F    +V   F P++  F +S  V+  + P  YL        C G  
Sbjct: 356 ---LHIVEETFQCFSFSTNVDEAFPPVSFEFEDS--VKLTVYPHDYLFTLEEELYCFGWQ 415

Query: 399 NGSQV--GLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPK 410
            G         V L+GD+ L +K+VVYD + + IGWA  NCS   K
Sbjct: 416 AGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCSSSIK 431

[Close]

BLAST of HG10013312 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.4e-31
Identity = 108/386 (27.98%), Postives = 169/386 (43.78%), Query Frame = 0


Query: 52  LGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRD----------RLYKPH 111
           +G +   + +G+PPK + + +DTGSD+ WV C APC  C +  D          +     
Sbjct: 75  IGLYFTKIKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTS 134

Query: 112 KNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTV- 171
           KN V C +  CS +  ++    K P   C Y V Y D  +S G  +KD + L    G + 
Sbjct: 135 KN-VGCEDDFCSFIMQSETCGAKKP---CSYHVVYGDGSTSDGDFIKDNITLEQVTGNLR 194

Query: 172 ---LAPNLGFGCGYDQHNGGSQLPPTA-GVLGLGNSKATMATQLSTLSNVRNIIGHCFSG 231
              LA  + FGCG +Q     Q      G++G G S  ++ +QL+   + + I  HC   
Sbjct: 195 TAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDN 254

Query: 232 QGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGGKPV----------GIRGV 291
             GG +F  G  V S  +  TPI+      Y+     +   G P+          G  G 
Sbjct: 255 MNGGGIFAVGE-VESPVVKTTPIVPNQ-VHYNVILKGMDVDGDPIDLPPSLASTNGDGGT 314

Query: 292 ILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVR 351
           I+  DSG++  Y    +Y +++  +           A +   L +  +    F   ++  
Sbjct: 315 II--DSGTTLAYLPQNLYNSLIEKI----------TAKQQVKLHMVQETFACFSFTSNTD 374

Query: 352 NFFKPLALSFTNSKNVQFQIPPEAYLIISNLGNVCLGILNG---SQVGLGNVNLIGDISL 410
             F  + L F +S  ++  + P  YL        C G  +G   +Q G  +V L+GD+ L
Sbjct: 375 KAFPVVNLHFEDS--LKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDG-ADVILLGDLVL 434

[Close]

BLAST of HG10013312 vs. ExPASy TrEMBL
Match: A0A5D3E3Y7 (Aspartic proteinase Asp1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold587G00060 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.5e-220
Identity = 369/411 (89.78%), Postives = 386/411 (93.92%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           M   NLSP+F+LF IFAVFLC   CLA  KS A N  DSSI+FPVKGNVYPLGHFTVSVT
Sbjct: 1   MPAFNLSPLFLLFVIFAVFLCGTFCLADWKSPAANPLDSSILFPVKGNVYPLGHFTVSVT 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPH NVVRCGEPLC+ALFSA K
Sbjct: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHNNVVRCGEPLCAALFSASK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
           SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGT+LAPNLGFGCGYDQHNGGSQ 
Sbjct: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGSQS 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP TAGVLGLGNSKATMATQLS LS+VRN++GHCFSGQG GFLFFGG+LVPSSGM W PI
Sbjct: 181 PPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGDGFLFFGGDLVPSSGMSWMPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           LRTPGGKYSAGPAEVYFGG PVGIRG+ILTFDSGSSYTYFN+QVYGAVLNLLRNGLKGQP
Sbjct: 241 LRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           L+DAPEDKTLP+CWKGSKAFKSVAD RNFFKPLALSF NSK VQFQIPPEAYLIISNLGN
Sbjct: 301 LRDAPEDKTLPMCWKGSKAFKSVADARNFFKPLALSFGNSKKVQFQIPPEAYLIISNLGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGILNGSQVGLGNVNLIGDIS++DKM+VYDNERQQIGWAPANCS+PPKK
Sbjct: 361 VCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSRPPKK 411

[Close]

BLAST of HG10013312 vs. ExPASy TrEMBL
Match: A0A1S3B9S6 (aspartic proteinase Asp1-like OS=Cucumis melo OX=3656 GN=LOC103487601 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.5e-220
Identity = 369/411 (89.78%), Postives = 386/411 (93.92%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           M   NLSP+F+LF IFAVFLC   CLA  KS A N  DSSI+FPVKGNVYPLGHFTVSVT
Sbjct: 1   MPAFNLSPLFLLFVIFAVFLCGTFCLADWKSPAANPLDSSILFPVKGNVYPLGHFTVSVT 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPH NVVRCGEPLC+ALFSA K
Sbjct: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHNNVVRCGEPLCAALFSASK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
           SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGT+LAPNLGFGCGYDQHNGGSQ 
Sbjct: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGSQS 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP TAGVLGLGNSKATMATQLS LS+VRN++GHCFSGQG GFLFFGG+LVPSSGM W PI
Sbjct: 181 PPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGDGFLFFGGDLVPSSGMSWMPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           LRTPGGKYSAGPAEVYFGG PVGIRG+ILTFDSGSSYTYFN+QVYGAVLNLLRNGLKGQP
Sbjct: 241 LRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           L+DAPEDKTLP+CWKGSKAFKSVAD RNFFKPLALSF NSK VQFQIPPEAYLIISNLGN
Sbjct: 301 LRDAPEDKTLPMCWKGSKAFKSVADARNFFKPLALSFGNSKKVQFQIPPEAYLIISNLGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGILNGSQVGLGNVNLIGDIS++DKM+VYDNERQQIGWAPANCS+PPKK
Sbjct: 361 VCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSRPPKK 411

[Close]

BLAST of HG10013312 vs. ExPASy TrEMBL
Match: A0A0A0L366 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361850 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 7.7e-220
Identity = 370/411 (90.02%), Postives = 387/411 (94.16%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCLAVRKSSATNAFDSSIVFPVKGNVYPLGHFTVSVT 60
           MH  NLSP+ +LF IF++  C   CLA  KSSA N FDSSI+ PVKGNVYPLGHFTVSVT
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLADWKSSAVNPFDSSILLPVKGNVYPLGHFTVSVT 60

Query: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADK 120
           IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLP DRLYKPH NVVRCGEPLCSALFSA K
Sbjct: 61  IGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSASK 120

Query: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQL 180
           SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGT+LAPNLGFGCGYDQHNGGSQL
Sbjct: 121 SPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGSQL 180

Query: 181 PP-TAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI 240
           PP TAGVLGLGNSKATMATQLS LS+VRN++GHCFSGQGGGFLFFGG+LVPSSGM W PI
Sbjct: 181 PPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWMPI 240

Query: 241 LRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQP 300
           LRTPGGKYSAGPAEVYFGG PVGIRG+ILTFDSGSSYTYFN+QVYGAVLNLLRNGLKGQP
Sbjct: 241 LRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKGQP 300

Query: 301 LKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLGN 360
           L+DAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSF NSK VQFQIPPEAYLIISNLGN
Sbjct: 301 LRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNLGN 360

Query: 361 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           VCLGILNGSQVGLGNVNLIGDIS++DKM+VYDNERQQIGWAPANCSKPP+K
Sbjct: 361 VCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

[Close]

BLAST of HG10013312 vs. ExPASy TrEMBL
Match: A0A6J1BSD8 (aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 4.0e-192
Identity = 320/412 (77.67%), Postives = 359/412 (87.14%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCL--AVRKSSATNAFDSSIVFPVKGNVYPLGHFTVS 60
           MH+  L PV +LFAIFAV   DK  L   ++  S +N F SS+ FP+KGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSA 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLPRD+ Y+PH N VRC EPLCSALF  
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 DKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D  P+RLTNG+V APNLGFGCGYDQ +GGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 QLPPTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTP 240
               TAGVLGLG+ KATM +QLS L +VRN++GHC SGQGGGFLFFGG+LVPSSGM WTP
Sbjct: 181 ----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTP 240

Query: 241 ILRTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQ 300
           I+ +PGG+YS+GPAEVYFGGK  GIRG+ LTFDSGSSYTYFN+QVYGAVLNLL+N LKG+
Sbjct: 241 IMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGK 300

Query: 301 PLKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQFQIPPEAYLIISNLG 360
           PL D P+DKTLP+CWKG+KAF+SV DVRNFFKPLALSFT+SKNVQFQIPPEAYLIIS  G
Sbjct: 301 PLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFG 360

Query: 361 NVCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPKK 411
           NVC GILNGSQVGLGNVN+IGDISL+DK+VVYDNERQQIGWAPANC++PPKK
Sbjct: 361 NVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

[Close]

BLAST of HG10013312 vs. ExPASy TrEMBL
Match: A0A6J1BT55 (aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.3e-187
Identity = 320/440 (72.73%), Postives = 359/440 (81.59%), Query Frame = 0


Query: 1   MHTLNLSPVFVLFAIFAVFLCDKVCL--AVRKSSATNAFDSSIVFPVKGNVYPLGHFTVS 60
           MH+  L PV +LFAIFAV   DK  L   ++  S +N F SS+ FP+KGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDRLYKPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDP 180
             PRD+ Y+PH N VRC EPLCSALF   K PCKNPNDQCDY+VEYAD GSSIGVLV+D 
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPLRLTNGTVLAPNLGFGCGYDQHNGGSQLPPTAGVLGLGNSKATMATQLSTLSNVRNII 240
            P+RLTNG+V APNLGFGCGYDQ +GGS    TAGVLGLG+ KATM +QLS L +VRN++
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGS----TAGVLGLGSGKATMTSQLSALGHVRNVV 240

Query: 241 GHCFSGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGGKPVGIRGVILTF 300
           GHC SGQGGGFLFFGG+LVPSSGM WTPI+ +PGG+YS+GPAEVYFGGK  GIRG+ LTF
Sbjct: 241 GHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTF 300

Query: 301 DSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVRNFFK 360
           DSGSSYTYFN+QVYGAVLNLL+N LKG+PL D P+DKTLP+CWKG+KAF+SV DVRNFFK
Sbjct: 301 DSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFK 360

Query: 361 PLALSFTNSKNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNLIGDISLIDKMVVY 411
           PLALSFT+SKNVQFQIPPEAYLIIS  GNVC GILNGSQVGLGNVN+IGDISL+DK+VVY
Sbjct: 361 PLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVY 420

[Close]

BLAST of HG10013312 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 476.5 bits (1225), Expect = 2.2e-134
Identity = 221/392 (56.38%), Postives = 287/392 (73.21%), Query Frame = 0


Query: 24  VCLAVRKSSATNAF----DSSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLT 83
           V + + KSS    F     SS+VFP+ GNV+PLG+++V + IG+PPK F+ DIDTGSDLT
Sbjct: 14  VIVPLSKSSIFKTFIKSSPSSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLT 73

Query: 84  WVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADH 143
           WVQCDAPC+GCTLP +  YKP  N++ C  P+C+AL   +K  C NP +QCDYEV+YAD 
Sbjct: 74  WVQCDAPCSGCTLPPNLQYKPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQ 133

Query: 144 GSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQLPP-TAGVLGLGNSKATMAT 203
           GSS+G LV D  PL+L NG+ + P + FGCGYDQ    +  PP TAGVLGLG  K  + T
Sbjct: 134 GSSMGALVTDQFPLKLVNGSFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLT 193

Query: 204 QLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPILRTPGGKYSAGPAEVYFGG 263
           QL +    RN++GHC S +GGGFLFFG NLVPS G+ WTP+L +    Y+ GPA++ F G
Sbjct: 194 QLVSAGLTRNVVGHCLSSKGGGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNG 253

Query: 264 KPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKA 323
           KP G++G+ L FD+GSSYTYFN++ Y  ++NL+ N LK  PLK A EDKTLPICWKG+K 
Sbjct: 254 KPTGLKGLKLIFDTGSSYTYFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKP 313

Query: 324 FKSVADVRNFFKPLALSFTNS-KNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNL 383
           FKSV +V+NFFK + ++FTN  +N Q  + PE YLI+S  GNVCLG+LNGS+VGL N N+
Sbjct: 314 FKSVLEVKNFFKTITINFTNGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNV 373

Query: 384 IGDISLIDKMVVYDNERQQIGWAPANCSKPPK 410
           IGDIS+   M++YDNE+QQ+GW  ++C+K PK
Sbjct: 374 IGDISMQGLMMIYDNEKQQLGWVSSDCNKLPK 404

[Close]

BLAST of HG10013312 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.4e-128
Identity = 219/410 (53.41%), Postives = 279/410 (68.05%), Query Frame = 0


Query: 10  FVLFAIFAVFLCDK--VCLAVRKSSA-----TNAFDSSIVFPVKGNVYPLGHFTVSVTIG 69
           F +F  +   LC +     A + SSA          S++VFPV GNVYPLG++ V + IG
Sbjct: 15  FFVFVFYVFILCARFQTSEATKDSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIG 74

Query: 70  NPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADKSP 129
           NPPK+F+LDIDTGSDLTWVQCDAPC GCT PR + YKP+ N + C   LCS L      P
Sbjct: 75  NPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRP 134

Query: 130 CKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQ-LP 189
           C +P DQCDYE+ Y+DH SSIG LV D VPL+L NG+++   L FGCGYDQ N G    P
Sbjct: 135 CADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPP 194

Query: 190 PTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI-L 249
           PTAG+LGLG  K  ++TQL +L   +N+I HC S  G GFL  G  LVPSSG+ WT +  
Sbjct: 195 PTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLAT 254

Query: 250 RTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPL 309
            +P   Y AGPAE+ F  K  G++G+ + FDSGSSYTYFN + Y A+L+L+R  L G+PL
Sbjct: 255 NSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPL 314

Query: 310 KDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQ-FQIPPEAYLIISNLGN 369
            D  +DK+LP+CWKG K  KS+ +V+ +FK + L F N KN Q FQ+PPE+YLII+  G 
Sbjct: 315 TDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGR 374

Query: 370 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPPK 410
           VCLGILNG+++GL   N+IGDIS    MV+YDNE+Q+IGW  ++C K PK
Sbjct: 375 VCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPK 424

[Close]

BLAST of HG10013312 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 5.2e-128
Identity = 218/409 (53.30%), Postives = 278/409 (67.97%), Query Frame = 0


Query: 10  FVLFAIFAVFLCDK--VCLAVRKSSA-----TNAFDSSIVFPVKGNVYPLGHFTVSVTIG 69
           F +F  +   LC +     A + SSA          S++VFPV GNVYPLG++ V + IG
Sbjct: 15  FFVFVFYVFILCARFQTSEATKDSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIG 74

Query: 70  NPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLYKPHKNVVRCGEPLCSALFSADKSP 129
           NPPK+F+LDIDTGSDLTWVQCDAPC GCT PR + YKP+ N + C   LCS L      P
Sbjct: 75  NPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRP 134

Query: 130 CKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTVLAPNLGFGCGYDQHNGGSQ-LP 189
           C +P DQCDYE+ Y+DH SSIG LV D VPL+L NG+++   L FGCGYDQ N G    P
Sbjct: 135 CADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPP 194

Query: 190 PTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQGGGFLFFGGNLVPSSGMLWTPI-L 249
           PTAG+LGLG  K  ++TQL +L   +N+I HC S  G GFL  G  LVPSSG+ WT +  
Sbjct: 195 PTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLAT 254

Query: 250 RTPGGKYSAGPAEVYFGGKPVGIRGVILTFDSGSSYTYFNNQVYGAVLNLLRNGLKGQPL 309
            +P   Y AGPAE+ F  K  G++G+ + FDSGSSYTYFN + Y A+L+L+R  L G+PL
Sbjct: 255 NSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPL 314

Query: 310 KDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFTNSKNVQ-FQIPPEAYLIISNLGN 369
            D  +DK+LP+CWKG K  KS+ +V+ +FK + L F N KN Q FQ+PPE+YLII+  G 
Sbjct: 315 TDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGR 374

Query: 370 VCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQQIGWAPANCSKPP 409
           VCLGILNG+++GL   N+IGDIS    MV+YDNE+Q+IGW  ++C K P
Sbjct: 375 VCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLP 423

[Close]

BLAST of HG10013312 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.2e-118
Identity = 199/371 (53.64%), Postives = 258/371 (69.54%), Query Frame = 0


Query: 39  SSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLY 98
           SS+VFPV GNVYPLG++ V++ IG PP+ + LD+DTGSDLTW+QCDAPC  C      LY
Sbjct: 44  SSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLY 103

Query: 99  KPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNG 158
           +P  +++ C +PLC AL       C+ P +QCDYEVEYAD GSS+GVLV+D   +  T G
Sbjct: 104 QPSSDLIPCNDPLCKALHLNSNQRCETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQG 163

Query: 159 TVLAPNLGFGCGYDQHNGGSQLPPTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQG 218
             L P L  GCGYDQ  G S   P  GVLGLG  K ++ +QL +   V+N+IGHC S  G
Sbjct: 164 LRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLG 223

Query: 219 GGFLFFGGNLVPSSGMLWTPILRTPGGKYS-AGPAEVYFGGKPVGIRGVILTFDSGSSYT 278
           GG LFFG +L  SS + WTP+ R     YS A   E+ FGG+  G++ ++  FDSGSSYT
Sbjct: 224 GGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYT 283

Query: 279 YFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFT 338
           YFN++ Y AV  LL+  L G+PLK+A +D TLP+CW+G + F S+ +V+ +FKPLALSF 
Sbjct: 284 YFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFK 343

Query: 339 NS--KNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNLIGDISLIDKMVVYDNERQ 398
                   F+IPPEAYLIIS  GNVCLGILNG+++GL N+NLIGDIS+ D+M++YDNE+Q
Sbjct: 344 TGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQ 403

Query: 399 QIGWAPANCSK 407
            IGW P +C +
Sbjct: 404 SIGWMPVDCDE 413

[Close]

BLAST of HG10013312 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 383.3 bits (983), Expect = 2.5e-106
Identity = 184/344 (53.49%), Postives = 236/344 (68.60%), Query Frame = 0


Query: 39  SSIVFPVKGNVYPLGHFTVSVTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPRDRLY 98
           SS+VFPV GNVYPLG++ V++ IG PP+ + LD+DTGSDLTW+QCDAPC  C      LY
Sbjct: 41  SSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLY 100

Query: 99  KPHKNVVRCGEPLCSALFSADKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNG 158
           +P  +++ C +PLC AL       C+ P +QCDYEVEYAD GSS+GVLV+D   +  T G
Sbjct: 101 QPSSDLIPCNDPLCKALHLNSNQRCETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQG 160

Query: 159 TVLAPNLGFGCGYDQHNGGSQLPPTAGVLGLGNSKATMATQLSTLSNVRNIIGHCFSGQG 218
             L P L  GCGYDQ  G S   P  GVLGLG  K ++ +QL +   V+N+IGHC S  G
Sbjct: 161 LRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLG 220

Query: 219 GGFLFFGGNLVPSSGMLWTPILRTPGGKYS-AGPAEVYFGGKPVGIRGVILTFDSGSSYT 278
           GG LFFG +L  SS + WTP+ R     YS A   E+ FGG+  G++ ++  FDSGSSYT
Sbjct: 221 GGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYT 280

Query: 279 YFNNQVYGAVLNLLRNGLKGQPLKDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFT 338
           YFN++ Y AV  LL+  L G+PLK+A +D TLP+CW+G + F S+ +V+ +FKPLALSF 
Sbjct: 281 YFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFK 340

Query: 339 NS--KNVQFQIPPEAYLIISNLGNVCLGILNGSQVGLGNVNLIG 380
                   F+IPPEAYLIIS  GNVCLGILNG+++GL N+NLIG
Sbjct: 341 TGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIG 383

The following BLAST results are available for this feature:

BLAST of HG10013312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444185.1	3.2e-220	89.78	PREDICTED: aspartic proteinase Asp1-like [Cucumis melo] >TYK30005.1 aspartic pro...	[more]
XP_004142705.1	1.6e-219	90.02	aspartic proteinase Asp1 [Cucumis sativus]	[more]
KAG6591878.1	1.3e-197	79.08	Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022132194.1	8.2e-192	77.67	aspartic proteinase Asp1-like isoform X4 [Momordica charantia]	[more]
XP_022132187.1	2.7e-187	72.73	aspartic proteinase Asp1-like isoform X3 [Momordica charantia]	[more]

BLAST of HG10013312 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0IU52	9.4e-82	42.56	Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S...	[more]
Q9M9A8	2.7e-81	42.50	Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1	[more]
A2ZC67	1.4e-80	42.30	Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=...	[more]
Q9S9K4	2.0e-31	27.09	Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2	[more]
Q4V3D2	3.4e-31	27.98	Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1	[more]

BLAST of HG10013312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E3Y7	1.5e-220	89.78	Aspartic proteinase Asp1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3B9S6	1.5e-220	89.78	aspartic proteinase Asp1-like OS=Cucumis melo OX=3656 GN=LOC103487601 PE=3 SV=1	[more]
A0A0A0L366	7.7e-220	90.02	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G36185...	[more]
A0A6J1BSD8	4.0e-192	77.67	aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1BT55	1.3e-187	72.73	aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of HG10013312 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44130.1	2.2e-134	56.38	Eukaryotic aspartyl protease family protein	[more]
AT1G77480.2	1.4e-128	53.41	Eukaryotic aspartyl protease family protein	[more]
AT1G77480.1	5.2e-128	53.30	Eukaryotic aspartyl protease family protein	[more]
AT4G33490.2	2.2e-118	53.64	Eukaryotic aspartyl protease family protein	[more]
AT4G33490.1	2.5e-106	53.49	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 268..279 score: 27.34 coord: 61..81 score: 46.1 coord: 376..391 score: 29.3
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 28..407
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 227..407 e-value: 2.7E-30 score: 107.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 35..225 e-value: 2.0E-40 score: 140.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 47..406
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 55..226 e-value: 2.3E-42 score: 145.2
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 269..400 e-value: 2.7E-14 score: 53.3
None	No IPR available	PANTHER	PTHR13683:SF227	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 28..407
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 70..81
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 55..400 score: 34.564198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10013312.1	HG10013312.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity