HG10012509 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012509
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionUbiquitin-conjugating enzyme E2 32
LocationChr01: 21952614 .. 21954842 (-)
RNA-Seq ExpressionHG10012509
SyntenyHG10012509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTGAAGGAGATGCAGGCTAATCCTTCCGATGATTTCATGAGTCTTCCTCTTGAGGTTTGTTGCTTTTGAATTTCTATGGATTTCCAATTCTGTTTTGTAATTTACCCTAATGGGTATTGTTCTTGTTTGGTCGGTTTTACATTCTAATGGTTTGCGTATCTTTGATGAATGAATTGACTCCTTGTGATTGTCTCTGCTTATTGAATTGGTATGATATATGTGGAAGTGTGGGTGGAAATCATTTATTAATTTGGTGTTCTTTGATTTTTCTTACCTGCTGGTGTCTGCAACTGTTTGCATATGAATGTTCTGTTAGGGCTCAAAAAGGGAGAAAAATGGTATTTATTCGAGTTTACAATCTTTGAATTTTTTTCGATAGCTGAAAATTTGGGGCAGTCCTTTGTTCTGAATAAACTCTTGCTCTCCAGTTAGGCCAATAATCAATTTTCTTTTAATTCATTACCGAATCTACAAACATACGTGCATCCAGTCCTTTTGCATAAATCTTCATGCATCTAATGCTATTCTCATGTGTCTTGATTCTTATAATTGTATTGAATTCATTGCATTTGGGAAGAAGAATAAAAGAAAAAGGAAAGGAAAATACTATGGGAAGCGTTGCTTGATTGATACCAGTTCTAGTATCGTCTGGGTAATTTTGTCTTTTTAATTGTTATGAAATTTAAGGGTTTTGCCAAAACTCTCTATTGAATTTGAGGCTAAATGTTGTCATTTCAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGCCCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCCTCATTCATGTTGTTAACCGTAAGATATCAATTTCTCTTCTCATGCTTGTTTCCTTTTCTGTTGCTTCTCGGCTTAGCATGTTTAGTTTTGATTTTGAAATGGTTATAAATCATTATTATCATAATGCACTTTTAAACACATTTTCATACAATCAAAATTCACTTTAAATAATTAAAATGAACGTTTCAGAGTGAACATAGGACTAGAATGAATGTAACAATTTCAAAACCACTCTCAAACGTGCCTATAGTTATTCTTTTGTGTATTTTGACTAGTGATTTTATACTACATTGAAGAGCTGATTTTATACTACATTGAAGAGCTGATCAATGTCAATTCTTTTCTTTCAGCCGAATGGGCGCTTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCATGGAGTGGTGTGTTCTTTGTATTCTTTATCCAACTTGTCTTGTCTTGTTATGTTATGTTGAACTCATAGGGTTTCCATTGTCTGGTTTCTACATTTACATTTAGTTGCTTCAAGTTCCACATGCTCTGATCTTTGTTCCTTTTTCCCGGCTTCCCCATTTTTGTTGATTACTGTTTGTAGTGCGCACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCCCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCTCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGGTACTCCTAAGTTCTAATTGATATTTTATAAGCTATCGAGTAATGTATCAAATCTCCAACATGTGATATGATATTGTTAAGTAATCAGCTTCTTTACGTCACAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCCCAATCGGGTTCTTCAGATGTCTCTGGGGAGAAACCCACCAAGGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCGACAGGATCATTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCGAGAGTGACAAGAGAGGTGCCATCTAGTCGCCCTACCGCTCAGGCTCAGCAGCAGAGGCCATCTGGTCGCCTTACTGCTCAGCAGCAGAGGCCAGAGACGAGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGGCTGACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTTTTCATGGATGAATCGTAG

mRNA sequence

ATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTGAAGGAGATGCAGGCTAATCCTTCCGATGATTTCATGAGTCTTCCTCTTGAGAAGAATAAAAGAAAAAGGAAAGGAAAATACTATGGGAAGCGTTGCTTGATTGATACCAGTTCTAGTATCGTCTGGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGCCCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCCTCATTCATGTTGTTAACCCCGAATGGGCGCTTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCATGGAGTGTGCGCACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCCCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCTCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCCCAATCGGGTTCTTCAGATGTCTCTGGGGAGAAACCCACCAAGGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCGACAGGATCATTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCGAGAGTGACAAGAGAGGTGCCATCTAGTCGCCCTACCGCTCAGGCTCAGCAGCAGAGGCCATCTGGTCGCCTTACTGCTCAGCAGCAGAGGCCAGAGACGAGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGGCTGACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTTTTCATGGATGAATCGTAG

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTGAAGGAGATGCAGGCTAATCCTTCCGATGATTTCATGAGTCTTCCTCTTGAGAAGAATAAAAGAAAAAGGAAAGGAAAATACTATGGGAAGCGTTGCTTGATTGATACCAGTTCTAGTATCGTCTGGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGCCCCGGTGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCCTCATTCATGTTGTTAACCCCGAATGGGCGCTTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCATGGAGTGTGCGCACTGCATTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCCCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTATTGGCCATCAAATCTCGAGAAGCACCTCCAAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATATGCTGAGTAAGGCGCCGCCTGTCCCCCAATCGGGTTCTTCAGATGTCTCTGGGGAGAAACCCACCAAGGAAGAGGCTGATGTCCAGGTAACTTCTGAACCTGCTGAACTTGCAGCTGCTGGGGAAGAGCTGCAGAATCCACCTGCAAGCGACAGGATCATTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCGAGAGTGACAAGAGAGGTGCCATCTAGTCGCCCTACCGCTCAGGCTCAGCAGCAGAGGCCATCTGGTCGCCTTACTGCTCAGCAGCAGAGGCCAGAGACGAGAGTTCATAAACCAGCTGATGACCGGTTGTTCACATGGGCTGCTATCGGGCTGACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTTTTCATGGATGAATCGTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSIVWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNPPASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Homology
BLAST of HG10012509 vs. NCBI nr
Match: XP_038895230.1 (ubiquitin-conjugating enzyme E2 32 [Benincasa hispida])

HSP 1 Score: 547.7 bits (1410), Expect = 6.8e-152
Identity = 287/337 (85.16%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPT EEADVQVTSEPAELAA+GEELQNP
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTNEEADVQVTSEPAELAASGEELQNP 240

Query: 241 PASDRIIEEAVPEVQPE-VARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPAD 300
           PA+DR++EE VPEV+PE VARV REVPSSRP AQ           A+Q+R ET VHKPAD
Sbjct: 241 PANDRVVEEVVPEVRPEVVARVAREVPSSRPPAQ-----------AEQRRLETGVHKPAD 300

Query: 301 DRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           DRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 DRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 301

BLAST of HG10012509 vs. NCBI nr
Match: KAA0049541.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa])

HSP 1 Score: 543.5 bits (1399), Expect = 1.3e-150
Identity = 285/336 (84.82%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGSSDVS EKPT EE+DVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q           A+QQRPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------AEQQRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. NCBI nr
Match: XP_008438945.1 (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 542.3 bits (1396), Expect = 2.9e-150
Identity = 284/336 (84.52%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGSSDVS +KPT EE+DVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q           A+QQRPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------AEQQRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. NCBI nr
Match: XP_004134243.1 (ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical protein Csa_009813 [Cucumis sativus])

HSP 1 Score: 535.8 bits (1379), Expect = 2.7e-148
Identity = 283/336 (84.23%), Postives = 287/336 (85.42%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGS+DVSGEKPT EEADVQVTSE AELAAA EELQN 
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSTDVSGEKPTNEEADVQVTSETAELAAAVEELQNS 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q            +Q RPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------TEQGRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. NCBI nr
Match: XP_022138234.1 (ubiquitin-conjugating enzyme E2 32 [Momordica charantia])

HSP 1 Score: 498.0 bits (1281), Expect = 6.2e-137
Identity = 268/344 (77.91%), Postives = 277/344 (80.52%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLE                       
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPSDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGS 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEY+LSKAPP+PQ          P+  +ADVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYILSKAPPIPQ----------PSPSDADVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPE-------VQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQ-QQRPET 300
           PA DRI+EEAVPE        QPEV RV REVPSSR              TAQ Q R ET
Sbjct: 241 PAGDRIVEEAVPEEVTTNANPQPEVVRVAREVPSSRAA------------TAQVQMRAET 297

Query: 301 RVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RV KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. ExPASy Swiss-Prot
Match: Q9LSP7 (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 7.9e-103
Identity = 200/335 (59.70%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGPGD+EFEGGIYHGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLS
Sbjct: 61  --ENIFEWQFAIRGPGDTEFEGGIYHGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISN+HPEHWQPSWSVRTALVALIAFMPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+
Sbjct: 121 ISNYHPEHWQPSWSVRTALVALIAFMPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGS 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQK+IDEIH+Y+LSKA  VP+    + S       EA  QV  EP E     EE ++ 
Sbjct: 181 PERQKIIDEIHQYILSKATVVPKPLPLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSI 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
             +D I+++ + E   E       V  +     A +      ++ +Q+       KP DD
Sbjct: 241 ATTDTIVDDQIIEETAEAVNTAASVVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDD 300

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 336
           RLFTWAA+GLT+AI+VLLLKKF+K++ + + FMD+
Sbjct: 301 RLFTWAAVGLTIAIMVLLLKKFIKSNGYSTGFMDD 307

BLAST of HG10012509 vs. ExPASy Swiss-Prot
Match: Q9JJZ4 (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.8e-47
Identity = 131/346 (37.86%), Postives = 177/346 (51.16%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSIVW 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE                         
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLE------------------------- 61

Query: 63  ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSIS 122
           +N+FEW F +RGP DS+F+GG+YHGRI LP EYP KPPS +LLT NGRFE   KICLSIS
Sbjct: 62  DNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSIS 121

Query: 123 NHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSRE--------- 182
            HHPE WQPSWS+RTAL+A+I FMPT   GA+GSLDY  EERR LA KS++         
Sbjct: 122 GHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSA 181

Query: 183 -----APPKFGTPERQ--KLIDEIHEYMLSKAPPVPQSG----SSDVSGEKPTKEEADVQ 242
                 P K G+   Q  +   E+   +  KA  V  SG     SD++      +  D  
Sbjct: 182 MKDVLLPLKSGSGSSQADQEAKELARQISFKA-EVNSSGKTIAESDLNQCFSLNDSQDDL 241

Query: 243 VTSEPAELAAAGEELQNPPASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGR 302
            T+     A+     QNP  +       +P+      + T   P  R   Q  Q+RPS  
Sbjct: 242 PTTFQGATASTSYGAQNPSGA------PLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTS 301

Query: 303 LTAQQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAH 329
               Q +P    H   +        I LTLA+  L+ ++   A+ +
Sbjct: 302 PDVLQGQPPRAHH--TEHGGSAMLIIILTLALAALIFRRIYLANEY 312

BLAST of HG10012509 vs. ExPASy Swiss-Prot
Match: Q9Y385 (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.9e-46
Identity = 132/350 (37.71%), Postives = 180/350 (51.43%), Query Frame = 0

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSIVW 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLE                         
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLE------------------------- 61

Query: 63  ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSIS 122
           +N+FEW F +RGP DS+F+GG+YHGRI LP EYP KPPS +LLT NGRFE   KICLSIS
Sbjct: 62  DNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSIS 121

Query: 123 NHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSRE--------- 182
            HHPE WQPSWS+RTAL+A+I FMPT   GA+GSLDY  EERR LA KS++         
Sbjct: 122 GHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSA 181

Query: 183 -----APPKFGTPERQ--KLIDEIHEYMLSKAPPVPQSG----SSDVSGEKPTKEEADVQ 242
                 P K G+   Q  +   E+   +  KA  V  SG     SD++      +  D  
Sbjct: 182 MKDVLLPLKSGSDSSQADQEAKELARQISFKA-EVNSSGKTISESDLNHSFSLTDLQDDI 241

Query: 243 VTSEPAELAAAGEELQNPPASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGR 302
            T+     A+    LQN  A+      +  +    VA+ T   P  R   Q  Q+R S  
Sbjct: 242 PTTFQGATASTSYGLQNSSAA------SFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTS 301

Query: 303 LTAQQQRPETRVHKPADDRLFTWAA----IGLTLAIVVLLLKKFMKASAH 329
               Q       H+P D+      +    + LTLA+  L+ ++   A+ +
Sbjct: 302 PDVIQG------HQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEY 312

BLAST of HG10012509 vs. ExPASy Swiss-Prot
Match: Q8N2K1 (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 124.8 bits (312), Expect = 1.9e-27
Identity = 51/108 (47.22%), Postives = 74/108 (68.52%), Query Frame = 0

Query: 64  NIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISN 123
           NI EW + +RGP  + +EGG YHG++  P E+PFKPPS  ++TPNGRF+  T++CLSI++
Sbjct: 40  NILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITD 99

Query: 124 HHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKS 172
            HP+ W P+WSV T L  L++FM       LGS++     +R LA++S
Sbjct: 100 FHPDTWNPAWSVSTILTGLLSFM-VEKGPTLGSIETSDFTKRQLAVQS 146

BLAST of HG10012509 vs. ExPASy Swiss-Prot
Match: Q6FQK7 (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.1e-27
Identity = 81/247 (32.79%), Postives = 127/247 (51.42%), Query Frame = 0

Query: 12  AVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSIVWENIFEWQFA 71
           A KR+ +E K M  NP    ++ P E                         ENI EW + 
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNE-------------------------ENILEWHYV 65

Query: 72  IRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQP 131
           I GP D+ ++GG YHG +  P++YP+KPP+  ++TPNGRF+  T++CLS+S++HP+ W P
Sbjct: 66  ISGPPDTPYDGGQYHGTLTFPSDYPYKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNP 125

Query: 132 SWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIH 191
            WSV T L  L++FM T      GS+   ++E+++LA KS      F +   + +  EI 
Sbjct: 126 GWSVATILNGLLSFM-TGDESTTGSITTTQQEKKILAKKSMYY-NTFNSTRFKLVFPEIV 185

Query: 192 EYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNPPASDRI-IEEA 251
           E  +++     Q        EK  KE+  V+   E    A   +++ NP   DRI  E+A
Sbjct: 186 EKNITELQRRKQEEKDMSMFEK--KEDPLVKAAREK---AIEVKDIVNP--EDRIRAEQA 218

Query: 252 VPEVQPE 258
           + E++ +
Sbjct: 246 LKELEKQ 218

BLAST of HG10012509 vs. ExPASy TrEMBL
Match: A0A5A7U7K5 (Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001470 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.2e-151
Identity = 285/336 (84.82%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGSSDVS EKPT EE+DVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q           A+QQRPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------AEQQRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. ExPASy TrEMBL
Match: A0A1S3AXJ7 (ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.4e-150
Identity = 284/336 (84.52%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGSSDVS +KPT EE+DVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q           A+QQRPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------AEQQRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. ExPASy TrEMBL
Match: A0A0A0L954 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.3e-148
Identity = 283/336 (84.23%), Postives = 287/336 (85.42%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEYMLSKAPPVPQSGS+DVSGEKPT EEADVQVTSE AELAAA EELQN 
Sbjct: 181 PERQKLIDEIHEYMLSKAPPVPQSGSTDVSGEKPTNEEADVQVTSETAELAAAVEELQNS 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
           P  +RI+EEAVPEVQPEVARV    PSSRPT Q            +Q RPETRVHKPADD
Sbjct: 241 PTINRIVEEAVPEVQPEVARV---APSSRPTTQ-----------TEQGRPETRVHKPADD 297

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. ExPASy TrEMBL
Match: A0A6J1C9I9 (ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC111009459 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 3.0e-137
Identity = 268/344 (77.91%), Postives = 277/344 (80.52%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAE+KYNLKNPAVKRILQEVKEMQ+NPSDDFMSLPLE                       
Sbjct: 1   MAEEKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGP DSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --ENIFEWQFAIRGPSDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFG+
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGS 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEY+LSKAPP+PQ          P+  +ADVQVTSEPAELAAAGEELQNP
Sbjct: 181 PERQKLIDEIHEYILSKAPPIPQ----------PSPSDADVQVTSEPAELAAAGEELQNP 240

Query: 241 PASDRIIEEAVPE-------VQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQ-QQRPET 300
           PA DRI+EEAVPE        QPEV RV REVPSSR              TAQ Q R ET
Sbjct: 241 PAGDRIVEEAVPEEVTTNANPQPEVVRVAREVPSSRAA------------TAQVQMRAET 297

Query: 301 RVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           RV KPADDRLFTWAA+GLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 301 RVPKPADDRLFTWAAVGLTLAIVVLLLKKFMKASAHGSVFMDES 297

BLAST of HG10012509 vs. ExPASy TrEMBL
Match: A0A6J1FCW1 (ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC111444487 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.1e-134
Identity = 258/340 (75.88%), Postives = 272/340 (80.00%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MAEDKYNLKNPAVKRIL+EVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             +NIFEWQFAIRGPGDSEFEGGIYHGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLS
Sbjct: 61  --DNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREA PKFGT
Sbjct: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGT 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQKLIDEIHEY+LSKAPP PQ  S D S EKP  +EADV+VTSEP E AA GEE QNP
Sbjct: 181 PERQKLIDEIHEYLLSKAPPAPQPSSPDDSEEKPANKEADVEVTSEPTEPAATGEEQQNP 240

Query: 241 PASDRIIEEAVPE---VQPEVAR-VTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHK 300
           P SDRI+EEA+PE   +   + +   RE PS RP              A Q R ETRV+K
Sbjct: 241 PGSDRIVEEAIPEETSINTNLQQAAAREAPSDRP--------------AVQLRTETRVNK 300

Query: 301 PADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 337
           PADDRLFTWAA+GLTLAIVVLLLKKF+KASAHGSVFMDES
Sbjct: 301 PADDRLFTWAAVGLTLAIVVLLLKKFIKASAHGSVFMDES 301

BLAST of HG10012509 vs. TAIR 10
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32 )

HSP 1 Score: 375.2 bits (962), Expect = 5.6e-104
Identity = 200/335 (59.70%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEKNKRKRKGKYYGKRCLIDTSSSI 60
           MA+++YN KNPAVKRILQEVKEMQANPSDDFMSLPLE                       
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLE----------------------- 60

Query: 61  VWENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLS 120
             ENIFEWQFAIRGPGD+EFEGGIYHGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLS
Sbjct: 61  --ENIFEWQFAIRGPGDTEFEGGIYHGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLS 120

Query: 121 ISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGT 180
           ISN+HPEHWQPSWSVRTALVALIAFMPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+
Sbjct: 121 ISNYHPEHWQPSWSVRTALVALIAFMPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGS 180

Query: 181 PERQKLIDEIHEYMLSKAPPVPQSGSSDVSGEKPTKEEADVQVTSEPAELAAAGEELQNP 240
           PERQK+IDEIH+Y+LSKA  VP+    + S       EA  QV  EP E     EE ++ 
Sbjct: 181 PERQKIIDEIHQYILSKATVVPKPLPLECSQAPSIVSEAHSQV--EPQEAITVVEE-RSI 240

Query: 241 PASDRIIEEAVPEVQPEVARVTREVPSSRPTAQAQQQRPSGRLTAQQQRPETRVHKPADD 300
             +D I+++ + E   E       V  +     A +      ++ +Q+       KP DD
Sbjct: 241 ATTDTIVDDQIIEETAEAVNTAASVVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDD 300

Query: 301 RLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 336
           RLFTWAA+GLT+AI+VLLLKKF+K++ + + FMD+
Sbjct: 301 RLFTWAAVGLTIAIMVLLLKKFIKSNGYSTGFMDD 307

BLAST of HG10012509 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-25
Identity = 74/202 (36.63%), Postives = 106/202 (52.48%), Query Frame = 0

Query: 37  EKNKRKRKGKYYGKRCLIDTSSSIVW---ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPA 96
           EK   KR  K Y   C    S  +      +I EW + + G   + F GG Y+G+I+ P 
Sbjct: 3   EKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPP 62

Query: 97  EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNG 156
           EYP+KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P  
Sbjct: 63  EYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP-- 122

Query: 157 ALGSLDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPV 216
             GS++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  
Sbjct: 123 TTGSVNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPES 182

Query: 217 PQSGSSDVSGEK---PTKEEAD 221
           PQ   + V  EK   PTK +++
Sbjct: 183 PQKSDTKVESEKTIDPTKGDSE 200

BLAST of HG10012509 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-25
Identity = 74/202 (36.63%), Postives = 106/202 (52.48%), Query Frame = 0

Query: 37  EKNKRKRKGKYYGKRCLIDTSSSIVW---ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPA 96
           EK   KR  K Y   C    S  +      +I EW + + G   + F GG Y+G+I+ P 
Sbjct: 3   EKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPP 62

Query: 97  EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNG 156
           EYP+KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P  
Sbjct: 63  EYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP-- 122

Query: 157 ALGSLDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPV 216
             GS++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  
Sbjct: 123 TTGSVNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPES 182

Query: 217 PQSGSSDVSGEK---PTKEEAD 221
           PQ   + V  EK   PTK +++
Sbjct: 183 PQKSDTKVESEKTIDPTKGDSE 200

BLAST of HG10012509 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-25
Identity = 65/191 (34.03%), Postives = 100/191 (52.36%), Query Frame = 0

Query: 37  EKNKRKRKGKYYGKRCLIDTSSSIVW---ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPA 96
           EK   KR  K Y   C    S  +      +I EW + + G   + F GG Y+G+I+ P 
Sbjct: 3   EKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPP 62

Query: 97  EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGA 156
           EYP+KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +  
Sbjct: 63  EYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPT 122

Query: 157 LGSLDYKKEERRVLAIKSREAPPKFGTPER--QKLIDEIHEYMLSKAPPVPQSGSSDVSG 216
            GS++    E++ LA  S     K  T  +   + +++  +  +++     Q  ++  + 
Sbjct: 123 TGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQ 182

Query: 217 EKPTKEEADVQ 223
           + P K+ A V+
Sbjct: 183 DFPQKDNAKVE 192

BLAST of HG10012509 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-25
Identity = 65/191 (34.03%), Postives = 100/191 (52.36%), Query Frame = 0

Query: 37  EKNKRKRKGKYYGKRCLIDTSSSIVW---ENIFEWQFAIRGPGDSEFEGGIYHGRIQLPA 96
           EK   KR  K Y   C    S  +      +I EW + + G   + F GG Y+G+I+ P 
Sbjct: 3   EKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPP 62

Query: 97  EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGA 156
           EYP+KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +  
Sbjct: 63  EYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPT 122

Query: 157 LGSLDYKKEERRVLAIKSREAPPKFGTPER--QKLIDEIHEYMLSKAPPVPQSGSSDVSG 216
            GS++    E++ LA  S     K  T  +   + +++  +  +++     Q  ++  + 
Sbjct: 123 TGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQ 182

Query: 217 EKPTKEEADVQ 223
           + P K+ A V+
Sbjct: 183 DFPQKDNAKVE 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895230.16.8e-15285.16ubiquitin-conjugating enzyme E2 32 [Benincasa hispida][more]
KAA0049541.11.3e-15084.82ubiquitin-conjugating enzyme E2 32 [Cucumis melo var. makuwa] >TYK16218.1 ubiqui... [more]
XP_008438945.12.9e-15084.52PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
XP_004134243.12.7e-14884.23ubiquitin-conjugating enzyme E2 32 [Cucumis sativus] >KGN57162.1 hypothetical pr... [more]
XP_022138234.16.2e-13777.91ubiquitin-conjugating enzyme E2 32 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LSP77.9e-10359.70Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2... [more]
Q9JJZ42.8e-4737.86Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2[more]
Q9Y3858.9e-4637.71Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2[more]
Q8N2K11.9e-2747.22Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3[more]
Q6FQK74.1e-2732.79Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
Match NameE-valueIdentityDescription
A0A5A7U7K56.2e-15184.82Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXJ71.4e-15084.52ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 ... [more]
A0A0A0L9541.3e-14884.23UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166270 PE... [more]
A0A6J1C9I93.0e-13777.91ubiquitin-conjugating enzyme E2 32 OS=Momordica charantia OX=3673 GN=LOC11100945... [more]
A0A6J1FCW13.1e-13475.88ubiquitin-conjugating enzyme E2 32-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G17000.15.6e-10459.70ubiquitin-conjugating enzyme 32 [more]
AT1G17280.11.0e-2536.63ubiquitin-conjugating enzyme 34 [more]
AT1G17280.21.0e-2536.63ubiquitin-conjugating enzyme 34 [more]
AT5G50430.11.8e-2534.03ubiquitin-conjugating enzyme 33 [more]
AT5G50430.21.8e-2534.03ubiquitin-conjugating enzyme 33 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..199
e-value: 8.9E-24
score: 95.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..291
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 58..336
coord: 7..39
NoneNo IPR availablePANTHERPTHR24068:SF303BNAC01G21910D PROTEINcoord: 58..336
coord: 7..39
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 59..151
e-value: 9.7E-17
score: 60.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 11..191
score: 32.232033
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 13..177
e-value: 2.01487E-39
score: 134.252
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..198
e-value: 5.6E-43
score: 148.5
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 12..159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012509.1HG10012509.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity