HG10012421 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012421
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionStrictosidine synthase-like
LocationChr01: 21047154 .. 21049908 (+)
RNA-Seq ExpressionHG10012421
SyntenyHG10012421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTGACAACAAATTGGTATATTTTATTTAGAATATAGTTTTCCTTTTAAATTCTAAAATTAAATTGATTTTTTTTTTGTAAATTTTGGGATTGAAATTCAGGTCAGAGAAGGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGTTGAAGGAACTCCCATTCTCTTTGCTAATGATCTCGATATCCACAACAATGGCTCCATCTTCTTCACAGACACTAGCAAGAGATATAATAGAGTGTAACTACTGATTTACATGACCTTTTAACTAAATTAATCGTTTAAATACTACTTTGGTTTCCGTATTTTTGTTTTTGGTTCATTTTAGTCTTTATACTTTCAAAATACAGGAACCGAAGTAGTATTTAAACCTAAATTAATTTATATCTAATAATAAGAAAAAATATTTACAGAAAATAGCAAAATTGTTAGATAGTTGTGATAGATGCTGATAGAAGTCTGTCAATGTCTATCAATGTTTTTCTTTTACTATTTTCCGTAAATAGTTTGACATTTTTTCTATCTGTAAAAATTTTCCTTTTTAATAATGTCACTTTCGTCGTGTTTTTGATTATGTGTGTGTTGTTTGATATAGGGATCATTTCTTCATATTGTTGGAAGGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAACTAAAACAACTCATGTTGTGTTGAATGGGTTGGCCTTTCCAAATGGGGTCCAATTGTCTAAAGACCATACCTTCCTTCTTTATACAGAAACTACCAATTGCAGGTATTTTAATTAGATCAAACCCTATATATAAATATTCAACATTATGCAAAGTCATATATATTATTTAAAGTTCTTATTAATACTCATTCTCTCACTTGTGCGTTAGTTTATATATTGTTTATTTTCAATTTTGATTCATTTTAGTTCTATAATTTCAAAATATCCTCCATTATTTAATAGTTTATGTCATTATACTTTCCACTTTGGTTCATTTTAATCTTTGTACTCATAATATTAAATTTTCGTTGACATCTCGACATTTTCATATTTCTATGATTTCGACATTAGCAGTAATGGTGAATTGACATTTCCACTATTTTGTACAAAAATTTCATGTAACATAAAAAATAAGCAACAAAATGTCACCAATGTGAATATAATATAAATAATAAAAAGAAAAAAGCTTGGGCGCAGCTTGGGCTATACTCATCTTTTGTGCAACCCAAGCTACACCCAAAAAAATATGCCAACAGGCAAATAATTTTTTTCAGTTAAAATATTTATTTTATTTTATTTTTACCATATTAAAAAAAATGGAGCATTGGATTAGGTGCAACCTATAATCATTGCCCTAATAAAAATGTTTTCTAATTTAAAAAGATGTAAGATGTATATTTATTAATTTAAACATTCTTATGGATTTTTTTTTTATATATAATTAAGCCAAAAATATCTATCAACATTGACATTTCCATGACCTCGACATTCAAATTTTGAACATTGCCTTCTACTTTCAAAAGAAGACTAACTTGATTGTTTTTCATGCAATATTCTAACACCAATTCTATACCCAATGAAAACCCTAAGATCCACAGCTACATTTCAAAAAAAAAAAAAAAAACAAAAAAACAAAACTATCGAGTATAGATTTATGTCGAATTTTTTCTCATAAAACAAAAGTATTGCAAGAGCATAAAAAAAAAAAACAAGTATACAGAGACGACGTAACAAATGAAAGTACAACAAAAGCTGAAAGTACAAGAACTAAAATAAAATATTATATTTTTCAATCCTTATATGCATGTTAATATATATACTACAATAATAATATCAATGGTATATATATATATATATACAGATTAATGAAGCTGTGGCTAGAGGGTGCAAGAAATGGAGAAGTTGAAGTAGTAGCAAATCTTCCAGGGTTTCCAGACAATGTAAGAAGAAATGAAAGGAATGAATATTGGGTAGCCATAGATTGTTGTAGAACAAAAGCACAAGAAGTTTTAACTAACAATCCATGGATAAGAAGCATTTATTTTAGGTTACCATTGAGAATGAGTTTCTTGGCCAGGCTAATGGGGATGAAAATGTATACTGTTATCTCACTTTTTAGTGAAAATGGAGACATTTTAGAAGTTCTTGAAGATCAAAAGGGTGAAGTTATGAAGCTAATGAGTGAAGTTAGAGAAGTTCAAGGCAAGCTTTGGATTGGAACTGTGGCTCATAATCATATTGCTACATTGACTTACCCTTTACAAATTAAACAATAA

mRNA sequence

ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTGACAACAAATTGGTCAGAGAAGGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGTTGAAGGAACTCCCATTCTCTTTGCTAATGATCTCGATATCCACAACAATGGCTCCATCTTCTTCACAGACACTAGCAAGAGATATAATAGAGTGGATCATTTCTTCATATTGTTGGAAGGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAACTAAAACAACTCATGTTGTGTTGAATGGGTTGGCCTTTCCAAATGGGGTCCAATTGTCTAAAGACCATACCTTCCTTCTTTATACAGAAACTACCAATTGCAGGCTAATGGGGATGAAAATGTATACTGTTATCTCACTTTTTAGTGAAAATGGAGACATTTTAGAAGTTCTTGAAGATCAAAAGGGTGAAGTTATGAAGCTAATGAGTGAAGTTAGAGAAGTTCAAGGCAAGCTTTGGATTGGAACTGTGGCTCATAATCATATTGCTACATTGACTTACCCTTTACAAATTAAACAATAA

Coding sequence (CDS)

ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTGACAACAAATTGGTCAGAGAAGGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGTTGAAGGAACTCCCATTCTCTTTGCTAATGATCTCGATATCCACAACAATGGCTCCATCTTCTTCACAGACACTAGCAAGAGATATAATAGAGTGGATCATTTCTTCATATTGTTGGAAGGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAACTAAAACAACTCATGTTGTGTTGAATGGGTTGGCCTTTCCAAATGGGGTCCAATTGTCTAAAGACCATACCTTCCTTCTTTATACAGAAACTACCAATTGCAGGCTAATGGGGATGAAAATGTATACTGTTATCTCACTTTTTAGTGAAAATGGAGACATTTTAGAAGTTCTTGAAGATCAAAAGGGTGAAGTTATGAAGCTAATGAGTGAAGTTAGAGAAGTTCAAGGCAAGCTTTGGATTGGAACTGTGGCTCATAATCATATTGCTACATTGACTTACCCTTTACAAATTAAACAATAA

Protein sequence

MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLMGMKMYTVISLFSENGDILEVLEDQKGEVMKLMSEVREVQGKLWIGTVAHNHIATLTYPLQIKQ
Homology
BLAST of HG10012421 vs. NCBI nr
Match: XP_038886083.1 (protein STRICTOSIDINE SYNTHASE-LIKE 13 [Benincasa hispida])

HSP 1 Score: 651.4 bits (1679), Expect = 4.4e-183
Identity = 329/410 (80.24%), Postives = 333/410 (81.22%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKKGVVKEESL QHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPV HDIAPYSQVMGRW
Sbjct: 1   MEKKGVVKEESLFQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVTHDIAPYSQVMGRW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+EVGWETFAIVT
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           TNWSEKVC +GVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA
Sbjct: 121 TNWSEKVCAQGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPST 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVLNGLAFPNGVQLSKDH+FLLYTETTNC                           
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHSFLLYTETTNCRLMKLWLEGARNGKVEVVANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 339
                                                        RLMGMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEVLTHNPWIRSIYFRLPFRMSFLARLMGMKMYTVISLFS 360

BLAST of HG10012421 vs. NCBI nr
Match: XP_008439075.1 (PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis melo])

HSP 1 Score: 639.0 bits (1647), Expect = 2.3e-179
Identity = 320/410 (78.05%), Postives = 331/410 (80.73%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MKKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAIVT
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
            NWSEKVC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRV+HFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVEHFFILLEGEASGRLLRYDPST 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM------------------------ 300
           KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM                        
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLMKLWLEGARNGKVEVVANLPGFPDN 300

Query: 301 ------------------------------------------------GMKMYTVISLFS 339
                                                           GMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEVLTHNPWIRSIYFRLPLRMSFLARLIGMKMYTVISLFS 360

BLAST of HG10012421 vs. NCBI nr
Match: XP_031738053.1 (protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis sativus] >KGN57252.1 hypothetical protein Csa_011798 [Cucumis sativus])

HSP 1 Score: 637.9 bits (1644), Expect = 5.0e-179
Identity = 320/410 (78.05%), Postives = 330/410 (80.49%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MQKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAIVT
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
            NWSEKVC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRV+HFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVEHFFILLEGEASGRLLRYDPST 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM------------------------ 300
           KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM                        
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLMKLWLEGARNGKVEVVANLPGFPDN 300

Query: 301 ------------------------------------------------GMKMYTVISLFS 339
                                                           GMKMYTVISLFS
Sbjct: 301 VRRNDRNEYWVAIDCCRTKAQEVLTHNPWIRSIYFRLPLRMSFLARLIGMKMYTVISLFS 360

BLAST of HG10012421 vs. NCBI nr
Match: XP_023526309.1 (protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 6.8e-176
Identity = 313/409 (76.53%), Postives = 328/409 (80.20%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKK  VKEES LQHPVLFLLVLGLGF+++DPF+MGPVGGHDFRPVKHDIAPYSQVMGRW
Sbjct: 1   MEKKRAVKEESFLQHPVLFLLVLGLGFILVDPFKMGPVGGHDFRPVKHDIAPYSQVMGRW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDN+SRLG GNLEFED+VFGPESLEFDALGRGPYTGLADGRIVRWMG+EVGWETFAIVT
Sbjct: 61  PKDNQSRLGLGNLEFEDQVFGPESLEFDALGRGPYTGLADGRIVRWMGEEVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           T WSEK C +GVDSTTAKQWKNEKKCGRPLGLRFEK++GNLYIADAYYGLL+VGPQGGIA
Sbjct: 121 TKWSEKECAQGVDSTTAKQWKNEKKCGRPLGLRFEKQTGNLYIADAYYGLLMVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHV+GTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVDGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPPT 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVLNGLAFPNGVQLSKDH+FLLYTETTNC                           
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHSFLLYTETTNCRLMKVWLGGSRNGEVEVVANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 338
                                                        RLMGMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEILTHNPWIRSIYFRLPLRMSLLARLMGMKMYTVISLFS 360

BLAST of HG10012421 vs. NCBI nr
Match: XP_022979805.1 (protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucurbita maxima])

HSP 1 Score: 626.7 bits (1615), Expect = 1.2e-175
Identity = 314/409 (76.77%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKK  VKEES LQHPVLFLLVL LGF++MDPF+MGPVGGHDFRPVKHDIAPYSQVMGRW
Sbjct: 1   MEKKRAVKEESFLQHPVLFLLVLVLGFILMDPFKMGPVGGHDFRPVKHDIAPYSQVMGRW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDN+SRLG GNLEFED+VFGPESLEFDALGRGPYTGLADGRIVRWMG+EVGWETFAIVT
Sbjct: 61  PKDNQSRLGLGNLEFEDQVFGPESLEFDALGRGPYTGLADGRIVRWMGEEVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           T WSEK C +GVDSTTAKQWKNEKKCGRPLGLRFEKE+GNLYIADAYYGLL+VGPQGGIA
Sbjct: 121 TKWSEKECAQGVDSTTAKQWKNEKKCGRPLGLRFEKETGNLYIADAYYGLLMVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHV+GTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVDGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPPT 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVLNGLAFPNGVQLSKDH+FLLYTETTNC                           
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHSFLLYTETTNCRLMKLWLGGSRNGKVEVVANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 338
                                                        RLMGMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEILAHNPWIRSIYFRLPLRMSLLARLMGMKMYTVISLFS 360

BLAST of HG10012421 vs. ExPASy Swiss-Prot
Match: Q9M1B4 (Protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Arabidopsis thaliana OX=3702 GN=SSL13 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 1.8e-139
Identity = 247/414 (59.66%), Postives = 288/414 (69.57%), Query Frame = 0

Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKKG     E  +  HP+L ++ L + F+ +DPF M P+GG +F+PVKH++APY +VMG
Sbjct: 1   MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKHEVAPYKEVMG 60

Query: 61  RWPKDNESRLG-HGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFA 120
            WP+DN SRLG HG LEF D+VFGPESLEFD+LGRGPYTGLADGR+VRWMG+ +GWETF+
Sbjct: 61  SWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTGLADGRVVRWMGEAIGWETFS 120

Query: 121 IVTTNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQG 180
           +VT+ WSE+ C +GVDSTT KQWK+EK CGRPLGLRF KE+GNLYIADAYYGLLVVGP+G
Sbjct: 121 VVTSKWSEEACVRGVDSTTNKQWKHEKLCGRPLGLRFHKETGNLYIADAYYGLLVVGPEG 180

Query: 181 GIATPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYD 240
           GIATPLATHVEG PILFANDLDIH NGSIFFTDTSKRY+R +HFFILLEGE++GRLLRYD
Sbjct: 181 GIATPLATHVEGKPILFANDLDIHRNGSIFFTDTSKRYDRANHFFILLEGESTGRLLRYD 240

Query: 241 PLTKTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRL---------------------- 300
           P TKTTH+VL GLAFPNG+QLSKD +FLL+TETTNCRL                      
Sbjct: 241 PPTKTTHIVLEGLAFPNGIQLSKDQSFLLFTETTNCRLVKYWLEGPKMGEVEVVADLPGF 300

Query: 301 --------------------------------------------------MGMKMYTVIS 340
                                                             MGM+MYTVIS
Sbjct: 301 PDNVRINEEGQFWVAIDCCRTPAQEVLTNNPWIRSIYFRLPIPMKLLAKTMGMRMYTVIS 360

BLAST of HG10012421 vs. ExPASy Swiss-Prot
Match: Q8VWF6 (Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana OX=3702 GN=SSL3 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 133/382 (34.82%), Postives = 192/382 (50.26%), Query Frame = 0

Query: 27  FVVMDPFEMGPVGGH-DFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESL 86
           +  +DPF    +    DF+  K D+ P S +     +D ++ L +  + F +EV GPES+
Sbjct: 16  YCAIDPFSHSSISKFPDFKTYKIDMPPLSSLPKE--RDRQNLLQNSEIRFLNEVQGPESI 75

Query: 87  EFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAKQWKNEKK 146
            FD  GRGPYTG+ADGRI+ W G    W  FA  + N SE    K    +     K+E  
Sbjct: 76  AFDPQGRGPYTGVADGRILFWNGTR--WTDFAYTSNNRSELCDPK---PSLLDYLKDEDI 135

Query: 147 CGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDLDIHNNGS 206
           CGRPLGLRF+K++G+LYIADAY G++ VGP+GG+AT +    +G P+ F NDLDI + G+
Sbjct: 136 CGRPLGLRFDKKNGDLYIADAYLGIMKVGPEGGLATSVTNEADGVPLRFTNDLDIDDEGN 195

Query: 207 IFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQLSKDHTFL 266
           ++FTD+S  + R     +++ GE SGR+L+Y+P TK T  ++  L FPNG+ L KD +F 
Sbjct: 196 VYFTDSSSFFQRRKFMLLIVSGEDSGRVLKYNPKTKETTTLVRNLQFPNGLSLGKDGSFF 255

Query: 267 LYTETTNCRL-------------------------------------------------- 326
           ++ E +  RL                                                  
Sbjct: 256 IFCEGSIGRLRKYWLKGEKAGTSEVVALLHGFPDNIRTNKDGDFWVAVHCHRNIFTHLMA 315

Query: 327 -----------------------MGMKMYTVISLFSENGDILEVLEDQKGEVMKLMSEVR 335
                                  +G   + V   +SE G +L+VLED KG+V+K +SEV 
Sbjct: 316 HYPRVRKFFLKLPISVKFQYLLQVGGWPHAVAVKYSEEGKVLKVLEDSKGKVVKAVSEVE 375

BLAST of HG10012421 vs. ExPASy Swiss-Prot
Match: Q4V3D9 (Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana OX=3702 GN=SSL10 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.0e-53
Identity = 123/318 (38.68%), Postives = 164/318 (51.57%), Query Frame = 0

Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAKQW 140
           GPES+ FD  G GPY G++DGRI++W G+ +GW  FA  ++N  E  C +          
Sbjct: 56  GPESIAFDPAGEGPYVGVSDGRILKWRGEPLGWSDFAHTSSNRQE--CARPFAP------ 115

Query: 141 KNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDLDI 200
           + E  CGRPLGLRF+K++G+LYIADAY+GLLVVGP GG+A PL T  EG P  F NDLDI
Sbjct: 116 ELEHVCGRPLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPLVTEAEGQPFRFTNDLDI 175

Query: 201 -HNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQLS 260
                 I+FTDTS R+ R      +L  + +GR ++YD  +K   V+L GLAF NGV LS
Sbjct: 176 DEQEDVIYFTDTSARFQRRQFLAAVLNVDKTGRFIKYDRSSKKATVLLQGLAFANGVALS 235

Query: 261 KDHTFLLYTETTNCRLM------------------------------------------- 320
           KD +F+L  ETT C+++                                           
Sbjct: 236 KDRSFVLVVETTTCKILRLWLSGPNAGTHQVFAELPGFPDNIRRNSNGEFWVALHSKKGL 295

Query: 321 -------------------------------GMKMYTVISLFSENGDILEVLEDQKGEVM 324
                                          G+   T I L SE+G +LEVLED++G+ +
Sbjct: 296 FAKLSLTQTWFRDLVLRLPISPQRLHSLFTGGIPHATAIKL-SESGKVLEVLEDKEGKTL 355

BLAST of HG10012421 vs. ExPASy Swiss-Prot
Match: Q9M1J7 (Protein STRICTOSIDINE SYNTHASE-LIKE 8 OS=Arabidopsis thaliana OX=3702 GN=SSL8 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 7.1e-43
Identity = 110/320 (34.38%), Postives = 157/320 (49.06%), Query Frame = 0

Query: 79  VFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAK 138
           V GPESLEFD  G GPY G+ DGRI++W G+E+GW  FA  + +       + V S    
Sbjct: 52  VDGPESLEFDPQGEGPYVGVTDGRILKWRGEELGWVDFAYTSPHRDNCSSHEVVPS---- 111

Query: 139 QWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDL 198
                  CGRPLGL FE+++G+LYI D Y+G++ VGP+GG+A  +    EG  ++FAN  
Sbjct: 112 -------CGRPLGLSFERKTGDLYICDGYFGVMKVGPEGGLAELVVDEAEGRKVMFANQG 171

Query: 199 DIHNNGSIF-FTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQ 258
           DI     IF F D+S  Y+  D F++ L G   GR++RYD   K   V+++ L  PNG+ 
Sbjct: 172 DIDEEEDIFYFNDSSDTYHFRDVFYVSLSGTKVGRVIRYDMKKKEAKVIMDKLRLPNGLA 231

Query: 259 LSKDHTFLLYTETT------------------------------------------NCR- 318
           LSK+ +F++  E++                                          +C+ 
Sbjct: 232 LSKNGSFVVTCESSTNICHRIWVKGPKSGTNEVFATLPGSPDNIRRTPTGDFWVALHCKK 291

Query: 319 -----------------LMGMKMYTVI-------------SLFSENGDILEVLEDQKGEV 324
                            +  MKM TVI              L  E G+ILE+LED +G+ 
Sbjct: 292 NLFTRAVLIHTWVGRFFMNTMKMETVIHFMNGGKPHGIVVKLSGETGEILEILEDSEGKT 351

BLAST of HG10012421 vs. ExPASy Swiss-Prot
Match: Q9M1J6 (Protein STRICTOSIDINE SYNTHASE-LIKE 9 OS=Arabidopsis thaliana OX=3702 GN=SSL9 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.3e-43
Identity = 110/320 (34.38%), Postives = 154/320 (48.12%), Query Frame = 0

Query: 79  VFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAK 138
           V GPES+EFD  G GPY  + DGRI++W G ++GW  FA  + +     C K     T  
Sbjct: 51  VAGPESIEFDPKGEGPYAAVVDGRILKWRGDDLGWVDFAYTSPHRGN--CSKTEVVPT-- 110

Query: 139 QWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDL 198
                  CGRPLGL FEK++G+LYI D Y GL+ VGP+GG+A  +    EG  ++FAN  
Sbjct: 111 -------CGRPLGLTFEKKTGDLYICDGYLGLMKVGPEGGLAELIVDEAEGRKVMFANQG 170

Query: 199 DIHNNGSIF-FTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQ 258
           DI     +F F D+S +Y+  D FF+ + GE SGR++RYD  TK   V+++ L   NG+ 
Sbjct: 171 DIDEEEDVFYFNDSSDKYHFRDVFFVAVSGERSGRVIRYDKKTKEAKVIMDNLVCNNGLA 230

Query: 259 LSKDHTFLLYTET----------------------------TNCRLMG------------ 318
           L+KD +FL+  E+                             N RL              
Sbjct: 231 LNKDRSFLITCESGTSLVHRYWIKGPKAGTRDIFAKVPGYPDNIRLTSTGDFWIGLHCKK 290

Query: 319 --------------------MKMYTVISLFS-------------ENGDILEVLEDQKGEV 324
                               MK+  VI+  +             E G++LE+LED++G+ 
Sbjct: 291 NLIGRLIVKYKWLGKLVEKTMKLEYVIAFINGFKPHGVAVKISGETGEVLELLEDKEGKT 350

BLAST of HG10012421 vs. ExPASy TrEMBL
Match: A0A1S3AYJ2 (protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucumis melo OX=3656 GN=LOC103483969 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.1e-179
Identity = 320/410 (78.05%), Postives = 331/410 (80.73%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MKKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAIVT
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
            NWSEKVC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRV+HFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVEHFFILLEGEASGRLLRYDPST 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM------------------------ 300
           KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM                        
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLMKLWLEGARNGKVEVVANLPGFPDN 300

Query: 301 ------------------------------------------------GMKMYTVISLFS 339
                                                           GMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEVLTHNPWIRSIYFRLPLRMSFLARLIGMKMYTVISLFS 360

BLAST of HG10012421 vs. ExPASy TrEMBL
Match: A0A0A0L9D9 (Str_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174550 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 320/410 (78.05%), Postives = 330/410 (80.49%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MQKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAIVT
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
            NWSEKVC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRV+HFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVEHFFILLEGEASGRLLRYDPST 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM------------------------ 300
           KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLM                        
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRLMKLWLEGARNGKVEVVANLPGFPDN 300

Query: 301 ------------------------------------------------GMKMYTVISLFS 339
                                                           GMKMYTVISLFS
Sbjct: 301 VRRNDRNEYWVAIDCCRTKAQEVLTHNPWIRSIYFRLPLRMSFLARLIGMKMYTVISLFS 360

BLAST of HG10012421 vs. ExPASy TrEMBL
Match: A0A6J1IUD9 (protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucurbita maxima OX=3661 GN=LOC111479397 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.6e-176
Identity = 314/409 (76.77%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKK  VKEES LQHPVLFLLVL LGF++MDPF+MGPVGGHDFRPVKHDIAPYSQVMGRW
Sbjct: 1   MEKKRAVKEESFLQHPVLFLLVLVLGFILMDPFKMGPVGGHDFRPVKHDIAPYSQVMGRW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDN+SRLG GNLEFED+VFGPESLEFDALGRGPYTGLADGRIVRWMG+EVGWETFAIVT
Sbjct: 61  PKDNQSRLGLGNLEFEDQVFGPESLEFDALGRGPYTGLADGRIVRWMGEEVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           T WSEK C +GVDSTTAKQWKNEKKCGRPLGLRFEKE+GNLYIADAYYGLL+VGPQGGIA
Sbjct: 121 TKWSEKECAQGVDSTTAKQWKNEKKCGRPLGLRFEKETGNLYIADAYYGLLMVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHV+GTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVDGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPPT 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVLNGLAFPNGVQLSKDH+FLLYTETTNC                           
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHSFLLYTETTNCRLMKLWLGGSRNGKVEVVANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 338
                                                        RLMGMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEILAHNPWIRSIYFRLPLRMSLLARLMGMKMYTVISLFS 360

BLAST of HG10012421 vs. ExPASy TrEMBL
Match: A0A6J1C8N3 (protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Momordica charantia OX=3673 GN=LOC111009399 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.2e-175
Identity = 311/409 (76.04%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKKG V+EES LQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW
Sbjct: 1   MEKKGAVREESFLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDN+SRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+ VGWETFAIVT
Sbjct: 61  PKDNQSRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGENVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           TNWSEK+C +GV+STT KQWK EKKCGRPLG+RFEK SGNLYIADAYYGLLVVGPQGGIA
Sbjct: 121 TNWSEKLCAQGVESTTEKQWKKEKKCGRPLGVRFEKGSGNLYIADAYYGLLVVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHV+GTPILFANDLDIHNNGS+FFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVQGTPILFANDLDIHNNGSVFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPPT 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVL+GLAFPNGVQLSKDH+FLL+TETTNC                           
Sbjct: 241 KTTHVVLDGLAFPNGVQLSKDHSFLLFTETTNCRLMKLWLEGARNGKVELLANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 338
                                                        RLMGM+MYTVISL S
Sbjct: 301 VRRNERNEYWVAIDCCRTRAQEVLTHNPWIRSIYFRLPLRMSFLARLMGMRMYTVISLIS 360

BLAST of HG10012421 vs. ExPASy TrEMBL
Match: A0A6J1GTQ7 (protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucurbita moschata OX=3662 GN=LOC111457451 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 310/409 (75.79%), Postives = 327/409 (79.95%), Query Frame = 0

Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKK  VKEES LQHPVLFLLVLGLGF+++DPF+MGPVGGHDFRPVKHDIAPYSQVMG W
Sbjct: 1   MEKKRAVKEESFLQHPVLFLLVLGLGFILVDPFKMGPVGGHDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVT 120
           PKDN+SRLG GNLEFED+VFGPESLEFDALGRGPYTGLADGRIVRWMG+EVGWETFAIVT
Sbjct: 61  PKDNQSRLGLGNLEFEDQVFGPESLEFDALGRGPYTGLADGRIVRWMGEEVGWETFAIVT 120

Query: 121 TNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 180
           T WSEK C +GVDSTTAKQWKNEKKCGRPLGLRFEK++GNLYIADAYYGLL+VGPQGGIA
Sbjct: 121 TKWSEKECAQGVDSTTAKQWKNEKKCGRPLGLRFEKQTGNLYIADAYYGLLMVGPQGGIA 180

Query: 181 TPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLT 240
           TPLATHV+GTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDP T
Sbjct: 181 TPLATHVDGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPPT 240

Query: 241 KTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNC--------------------------- 300
           KTTHVVLNGLAFPNGVQLSKDH+FLLYTETTNC                           
Sbjct: 241 KTTHVVLNGLAFPNGVQLSKDHSFLLYTETTNCRLMKVWLGGSRNGKVEVVANLPGFPDN 300

Query: 301 ---------------------------------------------RLMGMKMYTVISLFS 338
                                                        RLMGMKMYTVISLFS
Sbjct: 301 VRRNERNEYWVAIDCCRTKAQEILTHNPWIRSIYFRLPLRMSLLARLMGMKMYTVISLFS 360

BLAST of HG10012421 vs. TAIR 10
Match: AT3G59530.1 (Calcium-dependent phosphotriesterase superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-140
Identity = 247/414 (59.66%), Postives = 288/414 (69.57%), Query Frame = 0

Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKKG     E  +  HP+L ++ L + F+ +DPF M P+GG +F+PVKH++APY +VMG
Sbjct: 1   MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKHEVAPYKEVMG 60

Query: 61  RWPKDNESRLG-HGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFA 120
            WP+DN SRLG HG LEF D+VFGPESLEFD+LGRGPYTGLADGR+VRWMG+ +GWETF+
Sbjct: 61  SWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTGLADGRVVRWMGEAIGWETFS 120

Query: 121 IVTTNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQG 180
           +VT+ WSE+ C +GVDSTT KQWK+EK CGRPLGLRF KE+GNLYIADAYYGLLVVGP+G
Sbjct: 121 VVTSKWSEEACVRGVDSTTNKQWKHEKLCGRPLGLRFHKETGNLYIADAYYGLLVVGPEG 180

Query: 181 GIATPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYD 240
           GIATPLATHVEG PILFANDLDIH NGSIFFTDTSKRY+R +HFFILLEGE++GRLLRYD
Sbjct: 181 GIATPLATHVEGKPILFANDLDIHRNGSIFFTDTSKRYDRANHFFILLEGESTGRLLRYD 240

Query: 241 PLTKTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRL---------------------- 300
           P TKTTH+VL GLAFPNG+QLSKD +FLL+TETTNCRL                      
Sbjct: 241 PPTKTTHIVLEGLAFPNGIQLSKDQSFLLFTETTNCRLVKYWLEGPKMGEVEVVADLPGF 300

Query: 301 --------------------------------------------------MGMKMYTVIS 340
                                                             MGM+MYTVIS
Sbjct: 301 PDNVRINEEGQFWVAIDCCRTPAQEVLTNNPWIRSIYFRLPIPMKLLAKTMGMRMYTVIS 360

BLAST of HG10012421 vs. TAIR 10
Match: AT3G59530.2 (Calcium-dependent phosphotriesterase superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-140
Identity = 247/414 (59.66%), Postives = 288/414 (69.57%), Query Frame = 0

Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKKG     E  +  HP+L ++ L + F+ +DPF M P+GG +F+PVKH++APY +VMG
Sbjct: 1   MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKHEVAPYKEVMG 60

Query: 61  RWPKDNESRLG-HGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFA 120
            WP+DN SRLG HG LEF D+VFGPESLEFD+LGRGPYTGLADGR+VRWMG+ +GWETF+
Sbjct: 61  SWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTGLADGRVVRWMGEAIGWETFS 120

Query: 121 IVTTNWSEKVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQG 180
           +VT+ WSE+ C +GVDSTT KQWK+EK CGRPLGLRF KE+GNLYIADAYYGLLVVGP+G
Sbjct: 121 VVTSKWSEEACVRGVDSTTNKQWKHEKLCGRPLGLRFHKETGNLYIADAYYGLLVVGPEG 180

Query: 181 GIATPLATHVEGTPILFANDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYD 240
           GIATPLATHVEG PILFANDLDIH NGSIFFTDTSKRY+R +HFFILLEGE++GRLLRYD
Sbjct: 181 GIATPLATHVEGKPILFANDLDIHRNGSIFFTDTSKRYDRANHFFILLEGESTGRLLRYD 240

Query: 241 PLTKTTHVVLNGLAFPNGVQLSKDHTFLLYTETTNCRL---------------------- 300
           P TKTTH+VL GLAFPNG+QLSKD +FLL+TETTNCRL                      
Sbjct: 241 PPTKTTHIVLEGLAFPNGIQLSKDQSFLLFTETTNCRLVKYWLEGPKMGEVEVVADLPGF 300

Query: 301 --------------------------------------------------MGMKMYTVIS 340
                                                             MGM+MYTVIS
Sbjct: 301 PDNVRINEEGQFWVAIDCCRTPAQEVLTNNPWIRSIYFRLPIPMKLLAKTMGMRMYTVIS 360

BLAST of HG10012421 vs. TAIR 10
Match: AT5G22020.1 (Calcium-dependent phosphotriesterase superfamily protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.5e-59
Identity = 133/387 (34.37%), Postives = 205/387 (52.97%), Query Frame = 0

Query: 17  VLFLLVLGLGFVVMDPFEMGPVGGHDF-RPVKHDIAPYSQVMGRWPKDNESRLGHGNLEF 76
           + F L L L +  +DPF    +   DF   V H++         W +D+++ L    + F
Sbjct: 13  LFFSLSLAL-YCSIDPFHHCAIS--DFPNFVSHEVISPRPDEVPWERDSQNSLQKSKILF 72

Query: 77  EDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDST 136
            +++ GPES+ FD+LGRGPYTG+ADGR++ W G++  W  FA  ++N SE +C      +
Sbjct: 73  FNQIQGPESVAFDSLGRGPYTGVADGRVLFWDGEK--WIDFAYTSSNRSE-ICDP--KPS 132

Query: 137 TAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFA 196
                +NE  CGRPLGLRF+K +G+LYIADAY GLL VGP+GG+ATPL T  EG P+ F 
Sbjct: 133 ALSYLRNEHICGRPLGLRFDKRTGDLYIADAYMGLLKVGPEGGLATPLVTEAEGVPLGFT 192

Query: 197 NDLDIHNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNG 256
           NDLDI ++G+++FTD+S  Y R +   ++  G+ +GR+L+YDP+ K   V+++ L FPNG
Sbjct: 193 NDLDIADDGTVYFTDSSISYQRRNFLQLVFSGDNTGRVLKYDPVAKKAVVLVSNLQFPNG 252

Query: 257 VQLSKDHTFLLYTE------------------------------------------TTNC 316
           V +S+D +F ++ E                                            +C
Sbjct: 253 VSISRDGSFFVFCEGDIGSLRRYWLKGEKAGTTDVFAYLPGHPDNVRTNQKGEFWVALHC 312

Query: 317 R-------------------------------LMGMKMYTVISLFSENGDILEVLEDQKG 330
           R                                +G++ + ++  +S  G ++ VLED +G
Sbjct: 313 RRNYYSYLMARYPKLRMFILRLPITARTHYSFQIGLRPHGLVVKYSPEGKLMHVLEDSEG 372

BLAST of HG10012421 vs. TAIR 10
Match: AT1G08470.1 (strictosidine synthase-like 3 )

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-59
Identity = 133/382 (34.82%), Postives = 192/382 (50.26%), Query Frame = 0

Query: 27  FVVMDPFEMGPVGGH-DFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESL 86
           +  +DPF    +    DF+  K D+ P S +     +D ++ L +  + F +EV GPES+
Sbjct: 16  YCAIDPFSHSSISKFPDFKTYKIDMPPLSSLPKE--RDRQNLLQNSEIRFLNEVQGPESI 75

Query: 87  EFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAKQWKNEKK 146
            FD  GRGPYTG+ADGRI+ W G    W  FA  + N SE    K    +     K+E  
Sbjct: 76  AFDPQGRGPYTGVADGRILFWNGTR--WTDFAYTSNNRSELCDPK---PSLLDYLKDEDI 135

Query: 147 CGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDLDIHNNGS 206
           CGRPLGLRF+K++G+LYIADAY G++ VGP+GG+AT +    +G P+ F NDLDI + G+
Sbjct: 136 CGRPLGLRFDKKNGDLYIADAYLGIMKVGPEGGLATSVTNEADGVPLRFTNDLDIDDEGN 195

Query: 207 IFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQLSKDHTFL 266
           ++FTD+S  + R     +++ GE SGR+L+Y+P TK T  ++  L FPNG+ L KD +F 
Sbjct: 196 VYFTDSSSFFQRRKFMLLIVSGEDSGRVLKYNPKTKETTTLVRNLQFPNGLSLGKDGSFF 255

Query: 267 LYTETTNCRL-------------------------------------------------- 326
           ++ E +  RL                                                  
Sbjct: 256 IFCEGSIGRLRKYWLKGEKAGTSEVVALLHGFPDNIRTNKDGDFWVAVHCHRNIFTHLMA 315

Query: 327 -----------------------MGMKMYTVISLFSENGDILEVLEDQKGEVMKLMSEVR 335
                                  +G   + V   +SE G +L+VLED KG+V+K +SEV 
Sbjct: 316 HYPRVRKFFLKLPISVKFQYLLQVGGWPHAVAVKYSEEGKVLKVLEDSKGKVVKAVSEVE 375

BLAST of HG10012421 vs. TAIR 10
Match: AT3G57030.1 (Calcium-dependent phosphotriesterase superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-54
Identity = 123/318 (38.68%), Postives = 164/318 (51.57%), Query Frame = 0

Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVTTNWSEKVCGKGVDSTTAKQW 140
           GPES+ FD  G GPY G++DGRI++W G+ +GW  FA  ++N  E  C +          
Sbjct: 56  GPESIAFDPAGEGPYVGVSDGRILKWRGEPLGWSDFAHTSSNRQE--CARPFAP------ 115

Query: 141 KNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHVEGTPILFANDLDI 200
           + E  CGRPLGLRF+K++G+LYIADAY+GLLVVGP GG+A PL T  EG P  F NDLDI
Sbjct: 116 ELEHVCGRPLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPLVTEAEGQPFRFTNDLDI 175

Query: 201 -HNNGSIFFTDTSKRYNRVDHFFILLEGEASGRLLRYDPLTKTTHVVLNGLAFPNGVQLS 260
                 I+FTDTS R+ R      +L  + +GR ++YD  +K   V+L GLAF NGV LS
Sbjct: 176 DEQEDVIYFTDTSARFQRRQFLAAVLNVDKTGRFIKYDRSSKKATVLLQGLAFANGVALS 235

Query: 261 KDHTFLLYTETTNCRLM------------------------------------------- 320
           KD +F+L  ETT C+++                                           
Sbjct: 236 KDRSFVLVVETTTCKILRLWLSGPNAGTHQVFAELPGFPDNIRRNSNGEFWVALHSKKGL 295

Query: 321 -------------------------------GMKMYTVISLFSENGDILEVLEDQKGEVM 324
                                          G+   T I L SE+G +LEVLED++G+ +
Sbjct: 296 FAKLSLTQTWFRDLVLRLPISPQRLHSLFTGGIPHATAIKL-SESGKVLEVLEDKEGKTL 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886083.14.4e-18380.24protein STRICTOSIDINE SYNTHASE-LIKE 13 [Benincasa hispida][more]
XP_008439075.12.3e-17978.05PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis melo][more]
XP_031738053.15.0e-17978.05protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis sativus] >KGN57252.1 hypothetica... [more]
XP_023526309.16.8e-17676.53protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucurbita pepo subsp. pepo][more]
XP_022979805.11.2e-17576.77protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M1B41.8e-13959.66Protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Arabidopsis thaliana OX=3702 GN=SSL13 ... [more]
Q8VWF66.0e-5834.82Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana OX=3702 GN=SSL3 PE... [more]
Q4V3D92.0e-5338.68Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana OX=3702 GN=SSL10 ... [more]
Q9M1J77.1e-4334.38Protein STRICTOSIDINE SYNTHASE-LIKE 8 OS=Arabidopsis thaliana OX=3702 GN=SSL8 PE... [more]
Q9M1J69.3e-4334.38Protein STRICTOSIDINE SYNTHASE-LIKE 9 OS=Arabidopsis thaliana OX=3702 GN=SSL9 PE... [more]
Match NameE-valueIdentityDescription
A0A1S3AYJ21.1e-17978.05protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucumis melo OX=3656 GN=LOC103483969 P... [more]
A0A0A0L9D92.4e-17978.05Str_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174550 P... [more]
A0A6J1IUD95.6e-17676.77protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucurbita maxima OX=3661 GN=LOC1114793... [more]
A0A6J1C8N36.2e-17576.04protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GTQ71.1e-17475.79protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
AT3G59530.11.3e-14059.66Calcium-dependent phosphotriesterase superfamily protein [more]
AT3G59530.21.3e-14059.66Calcium-dependent phosphotriesterase superfamily protein [more]
AT5G22020.12.5e-5934.37Calcium-dependent phosphotriesterase superfamily protein [more]
AT1G08470.14.3e-5934.82strictosidine synthase-like 3 [more]
AT3G57030.11.4e-5438.68Calcium-dependent phosphotriesterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 297..317
NoneNo IPR availablePANTHERPTHR10426STRICTOSIDINE SYNTHASE-RELATEDcoord: 6..276
coord: 274..334
NoneNo IPR availablePANTHERPTHR10426:SF21PROTEIN STRICTOSIDINE SYNTHASE-LIKE 13coord: 6..276
coord: 274..334
NoneNo IPR availableSUPERFAMILY63829Calcium-dependent phosphotriesterasecoord: 74..326
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 68..279
e-value: 1.3E-84
score: 285.8
coord: 280..338
e-value: 9.7E-6
score: 26.6
IPR018119Strictosidine synthase, conserved regionPFAMPF03088Str_synthcoord: 196..275
e-value: 1.8E-25
score: 88.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012421.1HG10012421.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009058 biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0016844 strictosidine synthase activity