HG10012397 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10012397
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Chr01: 20782857 .. 20783444 (+)
RNA-Seq Expression	HG10012397
Synteny	HG10012397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAATCCGCTTCCCTTCTCCTACTACTCCTCTCCATTCTCACCATCTCCGCCGCTGCCGCCGCCCGTAGCGTCGGAGCTTCCAATTTCATCAAATCCAAGTGCAGCGCCGCTACTTACCCTGACCTATGCGTCCAATCTCTCTCAAATTTCGCCTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCGCCCGATCTTTCGTCTGGAAACTGACGAAGTTCAATGGACTGAAGCCGAGAGAGCACGCGGCCTTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTAGATCGGCTGAACAAATCGGTGGAGGAACTGAAGCGCGTTAGCGGATCGAATAAGAAGGACTTCCTATGGCACATTAGCAACGTTGAGACTTGGGTGAGTGCCGCGATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCCGCACTGAATGGAAGAATTAAATCTTCGATTAGGGCTCGCATTGTGGATGTTACTCGAGTGATAAGCAATGCGTTGTCTCTGATTAATAAGTATGCTGAAAATCAAAGTTAG

mRNA sequence

ATGGCGAAATCCGCTTCCCTTCTCCTACTACTCCTCTCCATTCTCACCATCTCCGCCGCTGCCGCCGCCCGTAGCGTCGGAGCTTCCAATTTCATCAAATCCAAGTGCAGCGCCGCTACTTACCCTGACCTATGCGTCCAATCTCTCTCAAATTTCGCCTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCGCCCGATCTTTCGTCTGGAAACTGACGAAGTTCAATGGACTGAAGCCGAGAGAGCACGCGGCCTTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTAGATCGGCTGAACAAATCGGTGGAGGAACTGAAGCGCGTTAGCGGATCGAATAAGAAGGACTTCCTATGGCACATTAGCAACGTTGAGACTTGGGTGAGTGCCGCGATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCCGCACTGAATGGAAGAATTAAATCTTCGATTAGGGCTCGCATTGTGGATGTTACTCGAGTGATAAGCAATGCGTTGTCTCTGATTAATAAGTATGCTGAAAATCAAAGTTAG

Coding sequence (CDS)

ATGGCGAAATCCGCTTCCCTTCTCCTACTACTCCTCTCCATTCTCACCATCTCCGCCGCTGCCGCCGCCCGTAGCGTCGGAGCTTCCAATTTCATCAAATCCAAGTGCAGCGCCGCTACTTACCCTGACCTATGCGTCCAATCTCTCTCAAATTTCGCCTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCGCCCGATCTTTCGTCTGGAAACTGACGAAGTTCAATGGACTGAAGCCGAGAGAGCACGCGGCCTTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTAGATCGGCTGAACAAATCGGTGGAGGAACTGAAGCGCGTTAGCGGATCGAATAAGAAGGACTTCCTATGGCACATTAGCAACGTTGAGACTTGGGTGAGTGCCGCGATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCCGCACTGAATGGAAGAATTAAATCTTCGATTAGGGCTCGCATTGTGGATGTTACTCGAGTGATAAGCAATGCGTTGTCTCTGATTAATAAGTATGCTGAAAATCAAAGTTAG

Protein sequence

MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEELKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVISNALSLINKYAENQS

Homology

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BLAST of HG10012397 vs. NCBI nr
Match: XP_038882396.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 329.7 bits (844), Expect = 1.7e-86
Identity = 179/197 (90.86%), Postives = 186/197 (94.42%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTI--SAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQ 60
           MAK AS LLLLLSILTI  +A A AR+ GASNFIKS+CS ATYPDLCVQSLSNFASTIQQ
Sbjct: 1   MAKPAS-LLLLLSILTIAATATATARNGGASNFIKSECSVATYPDLCVQSLSNFASTIQQ 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVE 120
           NPRQLVQTAL+VSLSRAQS RSFVWKLTKF+GLKPREHAALKDCMEEV DTVDRLNKSVE
Sbjct: 61  NPRQLVQTALIVSLSRAQSTRSFVWKLTKFSGLKPREHAALKDCMEEVSDTVDRLNKSVE 120

Query: 121 ELKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTR 180
           ELKRVSGS KKDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGRIKSS+RARIVDVTR
Sbjct: 121 ELKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRIKSSVRARIVDVTR 180

Query: 181 VISNALSLINKYAENQS 196
           VISNALSLINKYAENQS
Sbjct: 181 VISNALSLINKYAENQS 196

[Close]

BLAST of HG10012397 vs. NCBI nr
Match: XP_008439100.1 (PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like [Cucumis melo var. makuwa] >TYK23683.1 21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 327.8 bits (839), Expect = 6.4e-86
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           MAKS SLLLLLLSILTISA+A   + GASNFIKSKCSAATYPDLCVQSLS+FASTIQ+NP
Sbjct: 1   MAKSLSLLLLLLSILTISASAF--NGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           RQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRLNKSVEEL
Sbjct: 61  RQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           KRVSGS KKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDVTRVI
Sbjct: 121 KRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALSLINKYAENQS 196
           SNALSLINKYAE QS
Sbjct: 181 SNALSLINKYAETQS 193

[Close]

BLAST of HG10012397 vs. NCBI nr
Match: XP_023551839.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.6 bits (823), Expect = 4.6e-84
Identity = 176/197 (89.34%), Postives = 188/197 (95.43%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAA--AAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQ 60
           MAKSAS LLLLLSILTI+AA  +AAR+ GASNFIKSKCSAATYPDLCVQSLS FASTIQQ
Sbjct: 1   MAKSAS-LLLLLSILTIAAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQ 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVE 120
           NPRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK RE+AAL+DCMEEVGDTVDRL+KSVE
Sbjct: 61  NPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVE 120

Query: 121 ELKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTR 180
           ELKRVSGS +KDF WH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV+R
Sbjct: 121 ELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSR 180

Query: 181 VISNALSLINKYAENQS 196
           VISNALSLINKYAEN+S
Sbjct: 181 VISNALSLINKYAENES 196

[Close]

BLAST of HG10012397 vs. NCBI nr
Match: XP_022922994.1 (21 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cucurbita maxima] >KAG7014820.1 hypothetical protein SDJN02_22449, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.5 bits (820), Expect = 1.0e-83
Identity = 175/196 (89.29%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISA-AAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQN 60
           MAKSAS LLLLLSILTI+A  +AAR+ GASNFIKSKCSAATYPDLCVQSLS FASTIQQN
Sbjct: 1   MAKSAS-LLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK RE+AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRV 180
           LKRVSGS +KDF WH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALSLINKYAENQS 196
           ISNALSLINKYAEN+S
Sbjct: 181 ISNALSLINKYAENES 195

[Close]

BLAST of HG10012397 vs. NCBI nr
Match: XP_004148136.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 5.1e-83
Identity = 175/195 (89.74%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           MAKS SLLLLLLSILTISA+A   + GAS+FIKSKCSAATYPDLCVQSLS+F+STIQ+NP
Sbjct: 1   MAKSLSLLLLLLSILTISASAF--NGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           RQLVQTAL VSLS AQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRLNKSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           KRVSGS KKDF WHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDVTRVI
Sbjct: 121 KRVSGS-KKDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALSLINKYAENQS 196
           SNALSLINKYAENQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]

BLAST of HG10012397 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.6e-47
Identity = 99/191 (51.83%), Postives = 140/191 (73.30%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           ++KS  +   LL IL     AAA +  A+ FIK+ C+  TYP +C QSLS +A TIQ NP
Sbjct: 3   LSKSTLVFSALLVIL-----AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNP 62

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           ++L  TAL VSL+R Q A++F+ +L KF GLK R++AA+ DC+EEV D++DR+++S +E+
Sbjct: 63  QELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEM 122

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           K +S +   DF + +SNVETWVSAA+TDE TC DGFAG  ++G+IK S+RA++V V RV 
Sbjct: 123 KNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVT 182

Query: 181 SNALSLINKYA 192
           SNAL+L+N +A
Sbjct: 183 SNALALVNNFA 188

[Close]

BLAST of HG10012397 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.7e-45
Identity = 96/190 (50.53%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 7   LLLLLLSILTISAA---AAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNPRQL 66
           L LL  +ILT S++   AA  S  A NFI++ C A TYP +CV SL+ +A++IQ +PR+L
Sbjct: 11  LFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRL 70

Query: 67  VQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEELKRV 126
            +TAL V++++AQS + FVW+L +F  LK RE  A+KDC+EE+ D VDRL  S+ E+K  
Sbjct: 71  AETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMC 130

Query: 127 SGSNKKDFLW-HISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVISN 186
             +  +D  W H+SN +TW SAA+T+ NTCSDGFAG  ++GR+K+S+RARI+++ R  SN
Sbjct: 131 GSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSN 190

Query: 187 ALSLINKYAE 193
           AL+LIN +A+
Sbjct: 191 ALALINAFAK 200

[Close]

BLAST of HG10012397 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.5e-42
Identity = 93/192 (48.44%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 8   LLLLLSILTISAAAAA-------RSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 67
           L L+L +L +S AA          S G + FIK+ C   +YPD C QSLS++AS I++ P
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 68  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 127
           R+L +TAL VS++RA+SA+++V ++T + G+  R+H A+ DC+EE+GDTVDRL+ S++EL
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 126

Query: 128 KRV-SGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRV 187
           K +  G + +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS IR  IV V   
Sbjct: 127 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 186

Query: 188 ISNALSLINKYA 192
            SNAL+LIN +A
Sbjct: 187 TSNALALINDFA 198

[Close]

BLAST of HG10012397 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-38
Identity = 94/197 (47.72%), Postives = 137/197 (69.54%), Query Frame = 0


Query: 3   KSASLLLLLLSILTISAAAAARSVGASN-FIKSKCSAATYPDLCVQSLSNFASTIQQNPR 62
           K+   L++LLSI  + +++A  +   S+ FI S C    YP LCV +LS +A+ I+ N  
Sbjct: 4   KNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNND 63

Query: 63  Q-LVQTALVVSLSRAQSARSFVWKLTKFN-GLKPREHAALKDCMEEVGDTVDRLNKSVEE 122
           Q L QTAL++SL+RA+S   FV KLTK     K RE+ A+KDC+E +G++VDRL +SV+E
Sbjct: 64  QDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKE 123

Query: 123 LKRVSGS-NKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTR 182
           L R   +   +DF+W +SNV+TWVSAA+TDE TC DGF+  A+ G++K  IR ++V V +
Sbjct: 124 LARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQ 183

Query: 183 VISNALSLINKYAENQS 196
           V SNAL+L+N++AE +S
Sbjct: 184 VTSNALALVNQFAEKRS 200

[Close]

BLAST of HG10012397 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.7e-31
Identity = 81/198 (40.91%), Postives = 123/198 (62.12%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAA--ARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQ 60
           MA + +L L+ ++   I  A+    R  GA + + S C  A+YP LCV++LS+++     
Sbjct: 1   MAPTQNLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTIT 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVE 120
           N R L Q A+ +SLS AQSA   +  +    G K +E AAL DC+E +GD+VD L++++ 
Sbjct: 61  NRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLG 120

Query: 121 ELK--RVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 180
            LK  RVSG + K+F W +SN +TW SAA+TD++TC DGF G   +G IK+ ++  +  V
Sbjct: 121 VLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMD-DGEIKTEVKQWMTKV 180

Query: 181 TRVISNALSLINKYAENQ 195
            RV SNAL ++N+  E +
Sbjct: 181 ARVTSNALYMVNQLDETR 197

[Close]

BLAST of HG10012397 vs. ExPASy TrEMBL
Match: A0A5A7VRA6 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00490 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.1e-86
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           MAKS SLLLLLLSILTISA+A   + GASNFIKSKCSAATYPDLCVQSLS+FASTIQ+NP
Sbjct: 1   MAKSLSLLLLLLSILTISASAF--NGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           RQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRLNKSVEEL
Sbjct: 61  RQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           KRVSGS KKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDVTRVI
Sbjct: 121 KRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALSLINKYAENQS 196
           SNALSLINKYAE QS
Sbjct: 181 SNALSLINKYAETQS 193

[Close]

BLAST of HG10012397 vs. ExPASy TrEMBL
Match: A0A1S3AXX1 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.1e-86
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           MAKS SLLLLLLSILTISA+A   + GASNFIKSKCSAATYPDLCVQSLS+FASTIQ+NP
Sbjct: 1   MAKSLSLLLLLLSILTISASAF--NGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           RQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRLNKSVEEL
Sbjct: 61  RQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           KRVSGS KKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDVTRVI
Sbjct: 121 KRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALSLINKYAENQS 196
           SNALSLINKYAE QS
Sbjct: 181 SNALSLINKYAETQS 193

[Close]

BLAST of HG10012397 vs. ExPASy TrEMBL
Match: A0A6J1JDT9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.0e-84
Identity = 175/196 (89.29%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISA-AAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQN 60
           MAKSAS LLLLLSILTI+A  +AAR+ GASNFIKSKCSAATYPDLCVQSLS FASTIQQN
Sbjct: 1   MAKSAS-LLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK RE+AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRV 180
           LKRVSGS +KDF WH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALSLINKYAENQS 196
           ISNALSLINKYAEN+S
Sbjct: 181 ISNALSLINKYAENES 195

[Close]

BLAST of HG10012397 vs. ExPASy TrEMBL
Match: A0A6J1E526 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.0e-84
Identity = 175/196 (89.29%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISA-AAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQN 60
           MAKSAS LLLLLSILTI+A  +AAR+ GASNFIKSKCSAATYPDLCVQSLS FASTIQQN
Sbjct: 1   MAKSAS-LLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK RE+AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRV 180
           LKRVSGS +KDF WH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALSLINKYAENQS 196
           ISNALSLINKYAEN+S
Sbjct: 181 ISNALSLINKYAENES 195

[Close]

BLAST of HG10012397 vs. ExPASy TrEMBL
Match: A0A0A0L8D7 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176260 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.5e-83
Identity = 175/195 (89.74%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MAKSASLLLLLLSILTISAAAAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60
           MAKS SLLLLLLSILTISA+A   + GAS+FIKSKCSAATYPDLCVQSLS+F+STIQ+NP
Sbjct: 1   MAKSLSLLLLLLSILTISASAF--NGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 120
           RQLVQTAL VSLS AQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRLNKSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KRVSGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVI 180
           KRVSGS KKDF WHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDVTRVI
Sbjct: 121 KRVSGS-KKDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALSLINKYAENQS 196
           SNALSLINKYAENQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]

BLAST of HG10012397 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 2.3e-49
Identity = 109/200 (54.50%), Postives = 144/200 (72.00%), Query Frame = 0


Query: 1   MAKSASLLLLLLSI---LTISAAAAARSVGAS----NFIKSKCSAATYPDLCVQSLSNFA 60
           MAK    +LL +S    L ++AA AA   GAS    NFI+S C   TYP LCV SLS +A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  STIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRL 120
           + IQ +P++L +TA+ V+LSRAQS + FV +LT+  GLK RE  A+KDC+EE+ DTVDRL
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 NKSVEELKRVSGSNKKD-FLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRAR 180
            KSV+ELK    +  +D F +H+SN +TW SAA+TDENTCSDGF+G  ++GRIK+S+RAR
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRAR 180

Query: 181 IVDVTRVISNALSLINKYAE 193
           I++V    SNALSLIN +A+
Sbjct: 181 IMNVGHETSNALSLINAFAK 200

[Close]

BLAST of HG10012397 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 6.9e-46
Identity = 96/190 (50.53%), Postives = 138/190 (72.63%), Query Frame = 0


Query: 7   LLLLLLSILTISAA---AAARSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNPRQL 66
           L LL  +ILT S++   AA  S  A NFI++ C A TYP +CV SL+ +A++IQ +PR+L
Sbjct: 11  LFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRL 70

Query: 67  VQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEELKRV 126
            +TAL V++++AQS + FVW+L +F  LK RE  A+KDC+EE+ D VDRL  S+ E+K  
Sbjct: 71  AETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMC 130

Query: 127 SGSNKKDFLW-HISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVISN 186
             +  +D  W H+SN +TW SAA+T+ NTCSDGFAG  ++GR+K+S+RARI+++ R  SN
Sbjct: 131 GSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSN 190

Query: 187 ALSLINKYAE 193
           AL+LIN +A+
Sbjct: 191 ALALINAFAK 200

[Close]

BLAST of HG10012397 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.5e-43
Identity = 93/192 (48.44%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 8   LLLLLSILTISAAAAA-------RSVGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 67
           L L+L +L +S AA          S G + FIK+ C   +YPD C QSLS++AS I++ P
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 68  RQLVQTALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEEL 127
           R+L +TAL VS++RA+SA+++V ++T + G+  R+H A+ DC+EE+GDTVDRL+ S++EL
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 126

Query: 128 KRV-SGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRV 187
           K +  G + +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS IR  IV V   
Sbjct: 127 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 186

Query: 188 ISNALSLINKYA 192
            SNAL+LIN +A
Sbjct: 187 TSNALALINDFA 198

[Close]

BLAST of HG10012397 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 166.4 bits (420), Expect = 2.3e-41
Identity = 94/188 (50.00%), Postives = 130/188 (69.15%), Query Frame = 0


Query: 7   LLLLLLSILTISAAAAARSVGAS-NFIKSKCSAATYPDLCVQSLSNFASTIQQNPRQLVQ 66
           L L LL   TI+  +++ +  +S NFI S C    Y  LCV+ L+ FA  I++N  QL Q
Sbjct: 14  LFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQ 73

Query: 67  TALVVSLSRAQSARSFVWKLTKFNGLKPREHAALKDCMEEVGDTVDRLNKSVEELKRV-- 126
           TAL V+L R QS   +V KLTK   +K RE+ A+KDC+E +GD ++ L +S+ ELK+V  
Sbjct: 74  TALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGR 133

Query: 127 SGSNKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTRVISNA 186
           SG ++ +FLW +SNVETWVSAA+TDE TC DGF G  ++G +KS+IR R+V V RV SNA
Sbjct: 134 SGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNA 193

Query: 187 LSLINKYA 192
           L+L+N++A
Sbjct: 194 LALVNRFA 201

[Close]

BLAST of HG10012397 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 9.7e-40
Identity = 94/197 (47.72%), Postives = 137/197 (69.54%), Query Frame = 0


Query: 3   KSASLLLLLLSILTISAAAAARSVGASN-FIKSKCSAATYPDLCVQSLSNFASTIQQNPR 62
           K+   L++LLSI  + +++A  +   S+ FI S C    YP LCV +LS +A+ I+ N  
Sbjct: 4   KNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNND 63

Query: 63  Q-LVQTALVVSLSRAQSARSFVWKLTKFN-GLKPREHAALKDCMEEVGDTVDRLNKSVEE 122
           Q L QTAL++SL+RA+S   FV KLTK     K RE+ A+KDC+E +G++VDRL +SV+E
Sbjct: 64  QDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKE 123

Query: 123 LKRVSGS-NKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVTR 182
           L R   +   +DF+W +SNV+TWVSAA+TDE TC DGF+  A+ G++K  IR ++V V +
Sbjct: 124 LARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQ 183

Query: 183 VISNALSLINKYAENQS 196
           V SNAL+L+N++AE +S
Sbjct: 184 VTSNALALVNQFAEKRS 200

The following BLAST results are available for this feature:

BLAST of HG10012397 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882396.1	1.7e-86	90.86	21 kDa protein-like [Benincasa hispida]	[more]
XP_008439100.1	6.4e-86	91.28	PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like ...	[more]
XP_023551839.1	4.6e-84	89.34	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022922994.1	1.0e-83	89.29	21 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cu...	[more]
XP_004148136.1	5.1e-83	89.74	21 kDa protein [Cucumis sativus]	[more]

BLAST of HG10012397 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	3.6e-47	51.83	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9STY5	9.7e-45	50.53	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SB37	3.5e-42	48.44	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI72	1.4e-38	47.72	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q84WE4	4.7e-31	40.91	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of HG10012397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VRA6	3.1e-86	91.28	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G...	[more]
A0A1S3AXX1	3.1e-86	91.28	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1	[more]
A0A6J1JDT9	5.0e-84	89.29	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1	[more]
A0A6J1E526	5.0e-84	89.29	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1	[more]
A0A0A0L8D7	2.5e-83	89.74	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176260 PE=4 S...	[more]

BLAST of HG10012397 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62350.1	2.3e-49	54.50	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	6.9e-46	50.53	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	2.5e-43	48.44	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	2.3e-41	50.00	pectin methylesterase inhibitor 1	[more]
AT1G62770.1	9.7e-40	47.72	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 103..123
None	No IPR available	PANTHER	PTHR31080:SF87	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 9..193
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 9..193
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..191 e-value: 4.39492E-48 score: 152.207
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..186 e-value: 1.3E-54 score: 197.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..186 e-value: 2.8E-37 score: 128.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..190 e-value: 9.4E-44 score: 146.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..190 e-value: 3.1E-42 score: 146.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10012397.1	HG10012397.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0009641	shade avoidance
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity